FastQCFastQC Report
Thu 26 May 2016
SRR935963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529613
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC212110.8385077084913779No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT211220.8349893837515857No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT188720.7460429717905466No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT176640.6982886315021309No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG175110.6922402754887803No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA122090.4826429971699228No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT119800.4735902290192215No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC70940.2804381539784939No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA67350.26624625980337707No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC65950.26071181639246793No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG58400.23086535371220815No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG55310.21865004646955877No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG49830.1969866536897146No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC46870.1852852590495068No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA40860.16152668412124702No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT40530.16022213674581842No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA40010.15816648633605218No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC39690.15690147069927296No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA39150.15476675681220803No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC39080.15449003464166258No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT38030.15033920208348076No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT36060.14255144956955867No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC35780.14144456088737686No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT35220.13923078352301319No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT34640.13693794268135084No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG33840.1337754035894028No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG32300.1276875158374028No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT31880.12602718281413006No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG30690.12132290591485734No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA26810.10598459131890925No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC26300.10396847264779238No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC25620.10128031441963652No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA25370.10029202095340276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA36900.078.852732
GAGTAGT43450.076.942981
AGTCGTA9150.075.434692
GAGTCGT14750.072.319751
GAGTCAG103100.069.47711
AGTCCGT5000.069.022742
GAGTTAG21600.067.042231
GAGTAAG20150.065.655911
GATTGTG184750.061.6914639
AGTCGGT5900.061.5193942
GAGTCCG10950.060.409431
GGTATCA77650.059.631461
GTGGGGT122750.059.462847
TCGTAGG23000.059.2436562
TGGGGTA125500.059.2015048
ACGGGAT16150.057.0951165
GAGTACG11400.056.979351
AGTTAGT17150.056.5532652
GTAGTAG76600.056.0921861
TCAGTGG87200.056.020084
Produced by FastQC (version 0.11.5)