##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935962_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1250301 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.011891536518007 31.0 31.0 34.0 28.0 34.0 2 31.0641197599618 33.0 31.0 34.0 27.0 34.0 3 31.272507180270992 34.0 31.0 34.0 28.0 34.0 4 34.417868177342896 37.0 35.0 37.0 31.0 37.0 5 34.506566818710056 37.0 35.0 37.0 31.0 37.0 6 34.67624675978025 37.0 35.0 37.0 32.0 37.0 7 34.617879214685104 37.0 35.0 37.0 31.0 37.0 8 34.56552142244148 37.0 35.0 37.0 31.0 37.0 9 36.137198162682424 39.0 37.0 39.0 31.0 39.0 10-11 35.92935341169846 39.0 37.0 39.0 31.0 39.0 12-13 35.76068922603437 39.0 37.0 39.0 30.0 39.0 14-15 37.197855556382024 40.0 38.0 41.0 31.0 41.0 16-17 37.355408817556736 40.0 38.0 41.0 31.0 41.0 18-19 37.42887512686945 40.0 38.0 41.0 31.0 41.0 20-21 37.54030869366657 40.0 38.0 41.0 31.0 41.0 22-23 37.43319808590091 40.0 38.0 41.0 31.0 41.0 24-25 37.40358881581315 40.0 37.0 41.0 31.0 41.0 26-27 37.390608741415065 40.0 37.0 41.0 30.5 41.0 28-29 37.298364153911734 40.0 37.0 41.0 30.0 41.0 30-31 37.22696254741858 40.0 37.0 41.0 30.0 41.0 32-33 37.138520244325164 40.0 36.5 41.0 30.0 41.0 34-35 36.92762302837477 40.0 36.0 41.0 30.0 41.0 36-37 36.92300534031405 40.0 36.0 41.0 30.0 41.0 38-39 37.02444811289442 40.0 36.0 41.0 30.0 41.0 40-41 36.985557877663055 40.0 36.0 41.0 30.0 41.0 42-43 36.83511970317548 40.0 35.5 41.0 30.0 41.0 44-45 36.67216814191143 40.0 35.0 41.0 30.0 41.0 46-47 36.52210147796411 40.0 35.0 41.0 30.0 41.0 48-49 36.36585830132104 40.0 35.0 41.0 30.0 41.0 50-51 36.15522222248883 39.0 35.0 41.0 30.0 41.0 52-53 35.92117858019789 39.0 35.0 41.0 30.0 41.0 54-55 35.668413446042194 39.0 35.0 41.0 29.5 41.0 56-57 35.401546907504674 38.0 35.0 40.5 29.0 41.0 58-59 35.04663956919174 37.0 34.5 40.0 29.0 41.0 60-61 34.73203052704909 37.0 34.0 40.0 28.0 41.0 62-63 34.3292155249016 36.0 34.0 39.0 27.5 41.0 64-65 33.91022481786386 35.5 34.0 39.0 27.0 41.0 66-67 33.58271248283413 35.0 33.5 38.0 27.5 40.0 68-69 33.37478015293917 35.0 34.0 37.0 27.5 40.0 70-71 33.16127516494028 35.0 34.0 37.0 28.0 39.0 72-73 32.88206559860386 35.0 34.0 36.0 27.0 39.0 74-75 32.575347456332516 35.0 33.0 36.0 27.0 38.0 76-77 32.358058579494056 35.0 33.0 35.0 26.5 37.0 78-79 32.15097684477578 35.0 33.0 35.0 26.0 37.0 80-81 31.92800613612242 35.0 33.0 35.0 26.0 36.0 82-83 31.755742817129637 35.0 33.0 35.0 25.5 36.0 84-85 31.522015098764218 35.0 33.0 35.0 25.0 36.0 86-87 31.32042364198701 35.0 33.0 35.0 24.0 35.0 88-89 31.165585327053247 35.0 33.0 35.0 23.5 35.0 90-91 31.060802958647557 35.0 33.0 35.0 22.0 35.0 92-93 30.934209442366278 35.0 32.5 35.0 20.0 36.0 94-95 30.747547190636496 35.0 32.0 35.0 19.0 35.0 96-97 30.611096447975328 35.0 32.0 35.0 18.0 35.0 98-99 30.427901361352184 35.0 32.0 35.0 16.0 35.0 100-101 30.271614195301773 35.0 32.0 35.0 11.5 35.0 102-103 29.528440351563344 34.0 30.0 35.0 7.5 35.0 104-105 29.968955475521494 35.0 31.0 35.0 6.0 35.0 106-107 29.939704919055494 35.0 31.0 35.0 2.0 35.0 108-109 29.78433753152241 35.0 31.0 35.0 2.0 35.0 110-111 29.633001173317467 35.0 31.0 35.0 2.0 35.0 112-113 29.489408150517356 35.0 31.0 35.0 2.0 35.0 114-115 29.266502226263917 35.0 31.0 35.0 2.0 35.0 116-117 29.054009794441498 35.0 30.0 35.0 2.0 35.0 118-119 28.79480581076077 35.0 30.0 35.0 2.0 35.0 120-121 28.483005292325608 35.0 30.0 35.0 2.0 35.0 122-123 27.9783760070575 35.0 29.0 35.0 2.0 35.0 124-125 25.231252314442685 32.0 23.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11941156492499871 1101 2 0.20854183958306294 1101 3 0.19619164630175945 1101 4 0.1806553114521563 1101 5 0.16500503613097095 1101 6 0.10058147320313537 1101 7 0.14615442236564036 1101 8 0.17113156278352903 1101 9 0.13867477603778866 1101 10-11 0.3734415877766182 1101 12-13 0.1652262988979274 1101 14-15 0.3583258101252511 1101 16-17 0.24135491971044587 1101 18-19 0.33708665716843456 1101 20-21 0.3260581607266815 1101 22-23 0.24209592033952987 1101 24-25 0.18746351629748403 1101 26-27 0.27311312934155296 1101 28-29 0.27594711831448393 1101 30-31 0.19191457568805248 1101 32-33 0.20372280123462616 1101 34-35 0.027188098963222274 1101 36-37 0.0711660887272032 1101 38-39 0.17579452714412724 1101 40-41 0.053282666488506436 1101 42-43 0.006291729019928027 1101 44-45 0.002269523614060631 1101 46-47 0.06810299436826028 1101 48-49 0.09148132729884395 1101 50-51 0.1261719548600837 1101 52-53 0.19293050589914884 1101 54-55 0.09111749118213197 1101 56-57 0.07420331376074074 1101 58-59 -0.018493340750616483 1101 60-61 0.1636617794987103 1101 62-63 0.05465882579620285 1101 64-65 3.0624877108209603E-4 1101 66-67 -0.08727108882414569 1101 68-69 0.05432189984740177 1101 70-71 -0.10417769186594228 1101 72-73 0.0030370839531173033 1101 74-75 0.03794393832282594 1101 76-77 0.11950990830690955 1101 78-79 0.19046401530392387 1101 80-81 0.18866698304249496 1101 82-83 0.10567622159598145 1101 84-85 0.15807408750799468 1101 86-87 0.05669153281236916 1101 88-89 -0.013842897834749124 1101 90-91 -0.06352018408804483 1101 92-93 -0.07742766782013177 1101 94-95 -0.054613417347077586 1101 96-97 0.059224537607921235 1101 98-99 -0.07396240622342276 1101 100-101 0.1192040818999125 1101 102-103 0.18473534902442879 1101 104-105 -0.02482671012834814 1101 106-107 0.1143094847294357 1101 108-109 0.26042325533168764 1101 110-111 0.0696618702251186 1101 112-113 0.13571028445629452 1101 114-115 -0.06494603261037213 1101 116-117 0.05717397702122895 1101 118-119 -0.11064059132278459 1101 120-121 -0.18801045535052197 1101 122-123 -0.07564338899504719 1101 124-125 0.13530621624696337 1102 1 0.20990618037210496 1102 2 0.17980896995355877 1102 3 0.2728180714602537 1102 4 0.24967379634180986 1102 5 0.2554652409557647 1102 6 0.171691556331389 1102 7 0.1896831500914118 1102 8 0.1642370221174403 1102 9 0.17149505999991987 1102 10-11 0.2223064097264782 1102 12-13 0.28488462625079336 1102 14-15 0.23234054000009507 1102 16-17 0.34778041166705975 1102 18-19 0.2698724240836441 1102 20-21 0.3033381039101357 1102 22-23 0.35082887510871785 1102 24-25 0.43096904345934917 1102 26-27 0.41762373047383505 1102 28-29 0.3581331648426058 1102 30-31 0.2869716139551457 1102 32-33 0.3342795402712042 1102 34-35 0.37818513787595975 1102 36-37 0.26913944497257347 1102 38-39 0.17268462280920005 1102 40-41 0.18461204815800158 1102 42-43 0.05458703743311588 1102 44-45 0.08749683060683822 1102 46-47 0.1332884197314428 1102 48-49 0.16516599899845374 1102 50-51 0.15686285984881465 1102 52-53 0.23449073317953406 1102 54-55 0.25547220155289097 1102 56-57 0.3349531744331955 1102 58-59 0.19139212154190233 1102 60-61 0.22401783365747718 1102 62-63 0.40029170986397844 1102 64-65 0.22560234862857698 1102 66-67 0.21812460317878646 1102 68-69 0.36446395186278835 1102 70-71 0.3475145835169471 1102 72-73 0.34612571046199747 1102 74-75 0.2812428243764842 1102 76-77 0.287315081748865 1102 78-79 0.2898420699118063 1102 80-81 0.28834905727223514 1102 82-83 0.22441184762275412 1102 84-85 0.278561994657494 1102 86-87 0.3243469313601004 1102 88-89 0.2601366054958092 1102 90-91 0.327740475666463 1102 92-93 0.42648308166805293 1102 94-95 0.3125782537214299 1102 96-97 0.39389763846970993 1102 98-99 0.391934893323171 1102 100-101 0.500084795171805 1102 102-103 0.4141170894711337 1102 104-105 0.2448319542357602 1102 106-107 -0.058548906336291395 1102 108-109 0.06283817152053928 1102 110-111 0.05670928628891758 1102 112-113 -0.04970142090221685 1102 114-115 -0.03607339199952264 1102 116-117 -0.17520875991453266 1102 118-119 -0.2052314898196066 1102 120-121 -0.1548863291408722 1102 122-123 -0.27524610594426946 1102 124-125 -0.189293440994863 1103 1 0.1120399952640625 1103 2 0.12998359349384359 1103 3 0.19305829239002037 1103 4 0.22033886531819746 1103 5 0.17493577414099093 1103 6 0.24841986668948124 1103 7 0.22732219794816189 1103 8 0.18580584094902264 1103 9 0.2059202646899152 1103 10-11 0.2542276807008079 1103 12-13 0.2283032894392889 1103 14-15 0.1833436163869635 1103 16-17 0.278835944350746 1103 18-19 0.2381103930266235 1103 20-21 0.15203265743213734 1103 22-23 0.170157595268968 1103 24-25 0.2666682350339187 1103 26-27 0.24343636909108568 1103 28-29 0.14112022875236363 1103 30-31 0.136000858374409 1103 32-33 0.2775092432608943 1103 34-35 0.33165753060516323 1103 36-37 0.2667430784120839 1103 38-39 0.1634267551245685 1103 40-41 0.3070079351412289 1103 42-43 0.25791125091794953 1103 44-45 0.24779654613645619 1103 46-47 0.3187026174476486 1103 48-49 0.27500658683860735 1103 50-51 0.2040669412540126 1103 52-53 0.2897176019351022 1103 54-55 0.27885784145588843 1103 56-57 0.23882241735292808 1103 58-59 0.12054555621013918 1103 60-61 0.22046777980635568 1103 62-63 -0.03193270798156078 1103 64-65 0.12632850291534226 1103 66-67 0.116889050021868 1103 68-69 0.24839713391537543 1103 70-71 0.142447511933959 1103 72-73 0.12486463163604355 1103 74-75 0.25394305717409793 1103 76-77 0.1994099191671097 1103 78-79 0.1287387247252596 1103 80-81 0.07885450807317795 1103 82-83 0.12051734246375645 1103 84-85 0.17441199639680605 1103 86-87 0.1338579955792092 1103 88-89 0.110399072731731 1103 90-91 0.15002991000507393 1103 92-93 0.15766414876800638 1103 94-95 0.19437225096757516 1103 96-97 0.2307052614517282 1103 98-99 0.25565676417436123 1103 100-101 0.29081601879294183 1103 102-103 0.2560350810825831 1103 104-105 0.2138796319841525 1103 106-107 0.1351190863727858 1103 108-109 0.19137281641889814 1103 110-111 0.3082913002500227 1103 112-113 0.39150173039326575 1103 114-115 0.27821942910025754 1103 116-117 0.21716413775550336 1103 118-119 0.3094986246946654 1103 120-121 0.4178120123660989 1103 122-123 0.45856638812433914 1103 124-125 0.24601833995585665 1104 1 0.20807241223693396 1104 2 0.24866211316081532 1104 3 0.38762709089266423 1104 4 0.4071678443457998 1104 5 0.3302060432801568 1104 6 0.47934991342714994 1104 7 0.3899762329753358 1104 8 0.35253710805552885 1104 9 0.49975622378431694 1104 10-11 0.4754839188819844 1104 12-13 0.5546033840220517 1104 14-15 0.5574075270400414 1104 16-17 0.5794282295129705 1104 18-19 0.5450079815844902 1104 20-21 0.5051872728974871 1104 22-23 0.4521394809622379 1104 24-25 0.42257624572454233 1104 26-27 0.5395264757590752 1104 28-29 0.4507051204908237 1104 30-31 0.5252322977129822 1104 32-33 0.5638445762031878 1104 34-35 0.6549561192035966 1104 36-37 0.5594741768277416 1104 38-39 0.42034082645246684 1104 40-41 0.5445064623155815 1104 42-43 0.37670952821093806 1104 44-45 0.31272079299942135 1104 46-47 0.48588426336812773 1104 48-49 0.5390288527430229 1104 50-51 0.45796968775995595 1104 52-53 0.501784856787701 1104 54-55 0.6153294469871469 1104 56-57 0.5562659204051386 1104 58-59 0.46230677881960247 1104 60-61 0.6072718737143603 1104 62-63 0.577354173986997 1104 64-65 0.6302832409712877 1104 66-67 0.5786998883445733 1104 68-69 0.6714224368989505 1104 70-71 0.6899280303943414 1104 72-73 0.4872048501743933 1104 74-75 0.6178779515201853 1104 76-77 0.7368686237115156 1104 78-79 0.7817047863154443 1104 80-81 0.7912288832892784 1104 82-83 0.7357064725920068 1104 84-85 0.5622858607114125 1104 86-87 0.728425957066186 1104 88-89 0.7017957106329611 1104 90-91 0.6899096823625683 1104 92-93 0.6461630706274448 1104 94-95 0.6030119910548954 1104 96-97 0.6550002528143715 1104 98-99 0.6489227799862363 1104 100-101 0.6891005873789702 1104 102-103 0.4826530484608611 1104 104-105 0.395955649746913 1104 106-107 0.340482006707564 1104 108-109 0.597269076697291 1104 110-111 0.462098884513356 1104 112-113 0.5754101686057354 1104 114-115 0.43390611353311925 1104 116-117 0.3127255724518605 1104 118-119 0.38877237839443524 1104 120-121 0.2830502366824348 1104 122-123 0.2892566298737833 1104 124-125 0.35479399851012516 1105 1 0.06207217692187328 1105 2 0.08345973286801822 1105 3 -6.875863552977535E-4 1105 4 0.039993548719110095 1105 5 -0.03995949277440758 1105 6 0.06219544527343146 1105 7 0.06202547234117617 1105 8 0.035873688902754 1105 9 0.010013594966437722 1105 10-11 0.05487863002029769 1105 12-13 0.13571408979915844 1105 14-15 0.1439433967128565 1105 16-17 0.2856912208654592 1105 18-19 0.1187235379645486 1105 20-21 0.031735854563947896 1105 22-23 0.13508573761627218 1105 24-25 0.2943696889006304 1105 26-27 0.4308737914554044 1105 28-29 0.24218655954826573 1105 30-31 0.1827752660568791 1105 32-33 0.33024332816182067 1105 34-35 0.19158122806793898 1105 36-37 0.14716701223107975 1105 38-39 0.23622904432682645 1105 40-41 0.22471718135251706 1105 42-43 0.24373192734139337 1105 44-45 0.20807640199542732 1105 46-47 0.2704063216652557 1105 48-49 0.09485221579370773 1105 50-51 0.07254960368150876 1105 52-53 0.14332811273890655 1105 54-55 0.11295232907374952 1105 56-57 0.03227514500465389 1105 58-59 0.1496446495419903 1105 60-61 0.09429266050085516 1105 62-63 0.15366006364913432 1105 64-65 0.036393619921923914 1105 66-67 0.09911471518567794 1105 68-69 0.19606748778117122 1105 70-71 0.005634470494513266 1105 72-73 0.028700918607498238 1105 74-75 -0.07138212578517056 1105 76-77 0.018765175972319525 1105 78-79 -0.012854735633908376 1105 80-81 0.053313214480908755 1105 82-83 0.01715698452677117 1105 84-85 0.042099656772940364 1105 86-87 0.013141932406661283 1105 88-89 0.11415049851634862 1105 90-91 0.15168172307331673 1105 92-93 0.18523299717552888 1105 94-95 0.03485466363639489 1105 96-97 0.09240691272869839 1105 98-99 0.11073886445562664 1105 100-101 0.1313055268787231 1105 102-103 0.1804874962206071 1105 104-105 0.07757903732514038 1105 106-107 0.11827346277866368 1105 108-109 0.02011499451008092 1105 110-111 0.009243915163455796 1105 112-113 -0.021764681069647906 1105 114-115 -0.05586717472098712 1105 116-117 -0.16328723167573855 1105 118-119 -0.15327960631382354 1105 120-121 -0.052683351803807454 1105 122-123 -0.05199301139525048 1105 124-125 0.07518025137919437 1106 1 0.05487672610243166 1106 2 0.18572391538952004 1106 3 0.30913070976554735 1106 4 -0.36461933812634584 1106 5 0.1087961801292181 1106 6 0.12925710689768266 1106 7 0.20014753466116275 1106 8 0.2850504411479946 1106 9 0.43993413307458695 1106 10-11 0.42802904073686676 1106 12-13 0.5398086882321209 1106 14-15 0.6693399788287167 1106 16-17 0.6462299468777459 1106 18-19 0.41121501454964005 1106 20-21 0.567819585736629 1106 22-23 0.5556694359460224 1106 24-25 0.5424699729094726 1106 26-27 0.5875014195270793 1106 28-29 0.4891901296796206 1106 30-31 0.5563227912747024 1106 32-33 0.6339742848868326 1106 34-35 0.6012027365571626 1106 36-37 0.531909627146085 1106 38-39 0.48500058381437583 1106 40-41 0.44739748828243364 1106 42-43 0.4628172082804056 1106 44-45 0.5088504453438958 1106 46-47 0.5865207122369611 1106 48-49 0.5571144907259153 1106 50-51 0.4487617552052825 1106 52-53 0.43574310579089826 1106 54-55 0.5735714077861047 1106 56-57 0.5630561678504336 1106 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-1.0126010362791789 2216 76-77 -0.9567973788122544 2216 78-79 -1.2476409981262897 2216 80-81 -1.2205425442341564 2216 82-83 -1.4108559893590282 2216 84-85 -1.3387854199398141 2216 86-87 -1.2944064807525635 2216 88-89 -1.2415609330799349 2216 90-91 -1.2566876509109903 2216 92-93 -1.1743786346082459 2216 94-95 -1.0837230495552959 2216 96-97 -1.1212057116336922 2216 98-99 -1.1679250868007287 2216 100-101 -1.3129236814312897 2216 102-103 -1.2599108776272132 2216 104-105 -1.5239969619342908 2216 106-107 -1.2505371121509192 2216 108-109 -1.3783691398268765 2216 110-111 -1.480175067041742 2216 112-113 -1.8607694525831917 2216 114-115 -1.6487744544542444 2216 116-117 -1.5461120535268407 2216 118-119 -1.4750488664684767 2216 120-121 -1.3066645826362233 2216 122-123 -1.121007791035563 2216 124-125 -0.8783313635122774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17608.0 3 3869.0 4 2571.0 5 2005.0 6 2243.0 7 2547.0 8 2896.0 9 3278.0 10 3964.0 11 4847.0 12 4938.0 13 4652.0 14 4676.0 15 4617.0 16 4412.0 17 4303.0 18 4224.0 19 3977.0 20 3949.0 21 4196.0 22 5082.0 23 6803.0 24 9773.0 25 14800.0 26 18726.0 27 16740.0 28 17610.0 29 23052.0 30 25961.0 31 31458.0 32 42604.0 33 57533.0 34 90067.0 35 120164.0 36 237080.0 37 373130.0 38 69769.0 39 177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.75350541503809 11.629093723138102 7.875778168892792 15.74162269293101 2 12.691558506489612 29.391516509009456 41.99140051596904 15.925524468531888 3 34.41221615836662 20.597690153597828 20.55665714562239 24.433436542413173 4 14.41828505240322 15.9807825526312 44.69632369527899 24.90460869968659 5 16.75238148559911 33.896358386588496 28.568229262479306 20.78303086533309 6 31.634062517745726 37.987732554001 15.008865865099686 15.369339063153593 7 41.68182305338407 25.140406527083943 16.814631391876176 16.36313902765581 8 39.32035185324412 26.266222184542197 15.86770791509837 18.54571804711532 9 49.98724307186829 12.026943911906013 12.023104836355406 25.962708179870287 10-11 37.250550067543735 25.07128283509331 20.434519367736247 17.24364772962671 12-13 29.17825387646655 27.247438816732934 24.42467853740819 19.14962876939233 14-15 25.255518471152143 27.591276020734206 22.9493138052357 24.203891702877947 16-17 23.97946574464869 32.16521461632039 21.810028145222628 22.045291493808293 18-19 24.439474974426158 26.178936112184186 24.603515473473987 24.77807343991567 20-21 25.19469311789721 26.881047043871835 22.395807089652813 25.52845274857814 22-23 25.523893846361794 24.468907886980816 22.79307142840004 27.214126838257346 24-25 22.62019305751175 23.94707354469044 31.208684948664363 22.22404844913345 26-27 20.041014533845587 23.79258867053484 29.495712389580486 26.670684406039086 28-29 22.6376383722516 24.630350770333358 23.35044118746878 29.381569669946263 30-31 23.144586783502533 27.640824089559235 22.536253270212534 26.6783358567257 32-33 29.02980962184306 24.9017636553118 20.955074018176422 25.113352704668717 34-35 28.21348619252484 20.210053419136674 25.77919237047719 25.797268017861295 36-37 25.156062420169224 25.409361425768672 27.574160142237748 21.860416011824352 38-39 23.547449774094396 28.555563820232088 24.369251884146298 23.527734521527215 40-41 21.862455520710615 27.915997827723082 26.131227600393824 24.090319051172475 42-43 22.526055725781234 22.9076838297338 24.346537353805203 30.21972309067976 44-45 23.19777397602657 24.15162428887124 23.898805167715615 28.751796567386574 46-47 24.330101311604167 23.053768652508477 23.986823972787352 28.629306063100007 48-49 19.500944172643226 23.31150658921332 25.484463341227432 31.703085896916022 50-51 22.91156289565473 25.005378704807885 24.86901154202068 27.21404685751671 52-53 24.025534651255978 23.22696694635932 27.691691840604783 25.055806561779924 54-55 21.344500244341162 24.69925242001726 27.78930833455304 26.16693900108854 56-57 21.721167739012305 26.242375308576804 25.78612353149833 26.250333420912558 58-59 24.077162219337584 23.47506720381732 28.894402227943512 23.553368348901586 60-61 25.239282380802702 27.74571883090552 26.717006544824006 20.29799224346777 62-63 25.887406312559936 25.21716770601639 25.402443091703518 23.492982889720153 64-65 29.140072623732284 21.045845730556355 26.99047413379403 22.82360751191733 66-67 28.9556853823307 24.495430491198704 23.79111868085901 22.75776544561159 68-69 23.605485434563516 27.712802546751767 25.12749756042936 23.554214458255345 70-71 24.692863145457373 27.13415865800944 23.65642750490279 24.5165506916304 72-73 23.588447078837575 27.400336799572262 23.848744919713173 25.16247120187699 74-75 21.76092197481015 27.53094339562273 26.205220663165317 24.5029139664018 76-77 22.83740469768195 27.56915854026742 24.00948574239331 25.58395101965732 78-79 23.540046957162286 28.85234087972496 21.182591057983817 26.425021105128938 80-81 22.813885429688657 23.826131398420685 24.64954102611476 28.710442145775893 82-83 24.788071032495377 20.561088889795336 27.079919155467362 27.570920922241925 84-85 25.930587937425226 21.427431480097102 29.77521416031995 22.86676642215772 86-87 27.298365278984736 23.374706914361607 25.152116595195693 24.174811211457964 88-89 23.67517624118794 25.927183640817958 26.57327133643318 23.824368781560924 90-91 21.281492441813967 27.52947292649764 26.092297848516356 25.09673678317204 92-93 24.19311746072377 23.180374684030333 26.749152081400183 25.87735577384571 94-95 23.019737728863305 22.864792417678803 28.03998180972444 26.07548804373345 96-97 22.815536698535546 22.288872340782913 27.934847031779032 26.96074392890251 98-99 22.03408945031451 24.054493851125695 28.877054107134505 25.034362591425293 100-101 21.22900336282117 27.490393224929104 26.401845332620965 24.878758079628756 102-103 22.285513648313486 26.379047925259595 25.818023020056767 25.517415406370148 104-105 22.492122862155387 23.635172645186678 25.484975351826826 28.38772914083111 106-107 21.843393494211128 25.01398679422676 23.785508361483547 29.35711135007856 108-109 23.60663552216626 25.313984392558268 25.372810227297265 25.706569857978202 110-111 24.341338605663758 26.56888221316307 23.942474652103773 25.147304529069398 112-113 21.972523897522688 26.960500653250275 25.547824438638667 25.519151010588377 114-115 22.657001739686855 24.632166210082186 27.01009818232718 25.70073386790378 116-117 25.11875140466176 23.778714085648176 25.467107520509064 25.635426989181003 118-119 23.27880675194174 24.821034002639017 25.965407844767935 25.934751400651308 120-121 21.39471046908655 25.25036371823614 27.577160654284917 25.777765158392395 122-123 21.98787989312422 26.441317302766315 28.34507711141052 23.225725692698944 124-125 22.47418489536248 28.053101921805602 24.756481889436742 24.716231293395175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 3.0 2 7.5 3 13.5 4 21.0 5 26.5 6 31.5 7 38.0 8 51.5 9 68.5 10 71.0 11 79.5 12 117.5 13 149.5 14 199.0 15 261.5 16 319.0 17 421.5 18 529.0 19 618.5 20 734.0 21 908.5 22 1117.5 23 1382.0 24 1709.5 25 2089.5 26 2446.5 27 2888.0 28 3385.5 29 3945.0 30 4739.0 31 5489.5 32 6406.5 33 7470.5 34 8647.5 35 9612.0 36 10768.0 37 12006.5 38 12735.0 39 13504.5 40 14030.0 41 15178.0 42 16646.0 43 18386.0 44 20802.0 45 25513.0 46 40984.5 47 70184.0 48 97185.0 49 115969.0 50 116235.5 51 93289.5 52 69262.0 53 54602.0 54 47688.0 55 43642.0 56 39326.5 57 35333.5 58 32328.0 59 28478.5 60 25068.5 61 21441.0 62 16867.5 63 13305.5 64 10808.0 65 9516.0 66 8436.5 67 7403.0 68 6237.0 69 4843.0 70 3701.0 71 2715.5 72 2105.5 73 1733.5 74 1351.5 75 1081.5 76 831.0 77 628.0 78 520.0 79 404.5 80 348.5 81 337.5 82 243.0 83 108.5 84 44.5 85 34.0 86 29.0 87 17.5 88 10.5 89 11.0 90 10.0 91 8.5 92 7.5 93 5.0 94 2.5 95 1.5 96 2.0 97 1.5 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16140113460678668 2 0.018075647384109904 3 0.0070383051761135915 4 0.014956398499241382 5 0.002479402959767288 6 0.0 7 2.3994222191296333E-4 8 5.598651844635811E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.997592579706806E-4 28-29 0.007638160730896 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.59913332869445E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0016795955533907435 66-67 1.9995185159413616E-4 68-69 0.006478439991650011 70-71 0.019795233307819477 72-73 1.9995185159413616E-4 74-75 0.009197785173330263 76-77 2.7993259223179056E-4 78-79 9.997592579706806E-4 80-81 9.597688876518533E-4 82-83 0.0 84-85 0.019555291085906513 86-87 0.002119489626897843 88-89 0.019075406642080586 90-91 7.998074063765445E-5 92-93 0.014476514055415456 94-95 0.014596485166371936 96-97 0.00591857480718643 98-99 0.002519393330086115 100-101 9.197785173330262E-4 102-103 0.0 104-105 4.3989407350709947E-4 106-107 0.0011597207392459897 108-109 0.0 110-111 0.0 112-113 0.0011597207392459897 114-115 0.006078536288461739 116-117 0.0 118-119 0.0777012895294813 120-121 0.0015996148127530893 122-123 0.005158757771128712 124-125 0.24910001671597481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1250301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.703403116340965 #Duplication Level Percentage of deduplicated Percentage of total 1 81.88958738038272 38.24522410456843 2 9.97563337993465 9.317920541678298 3 2.9095182215645083 4.076532071282 4 1.38119970754398 2.5802690690239496 5 0.779728535640808 1.8207988060673443 6 0.5138183733619721 1.4398239971824065 7 0.361549584823215 1.1819917204581025 8 0.28427798063535126 1.0621399301369743 9 0.21399070702602016 0.8994684828048334 >10 1.4209350426639855 12.70966814274012 >50 0.1458658556308662 4.70588581698199 >100 0.10864395196319074 10.070132738840721 >500 0.008910859540433816 2.7467853027226754 >1k 0.005140880504096432 4.309254038046669 >5k 8.568134173494051E-4 3.0958857802867414 >10k+ 3.427253669397621E-4 1.7382194571788054 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 11049 0.8837072033054441 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10670 0.853394502603773 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9935 0.794608658235097 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 9182 0.7343831605349431 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7782 0.622410123642227 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6290 0.5030788586108466 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5494 0.43941418906327356 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3699 0.29584875961868384 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3327 0.26609592410147637 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3061 0.24482104709186028 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2723 0.21778755675633307 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2711 0.21682778786868123 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2261 0.18083645458173672 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2094 0.16747967089524843 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2093 0.16739969015461076 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2085 0.1667598442295095 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2043 0.16340065312272803 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1999 0.15988150053467126 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1918 0.15340306054302125 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1700 0.13596725908401255 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1630 0.13036860723937674 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1575 0.12596966650430574 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1488 0.11901134206882982 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1464 0.11709180429352611 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1441 0.11525224725886006 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1429 0.11429247837120822 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1428 0.11421249763057055 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1342 0.10733415393573227 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1326 0.10605446208552981 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1313 0.1050147124572403 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.999037031882723E-5 0.0 12-13 0.0 0.0 0.0 7.998074063765445E-5 0.0 14-15 0.0 0.0 0.0 7.998074063765445E-5 0.0 16-17 0.0 0.0 0.0 7.998074063765445E-5 0.0 18-19 0.0 0.0 0.0 1.599614812753089E-4 0.0 20-21 0.0 0.0 0.0 6.798362954200628E-4 0.0 22-23 0.0 0.0 0.0 0.002079499256579016 0.0 24-25 0.0 0.0 0.0 0.0040790177725203765 0.0 26-27 0.0 0.0 0.0 0.006798362954200629 0.0 28-29 0.0 0.0 0.0 0.014596485166371938 0.0 30-31 0.0 0.0 0.0 0.04242978290827569 0.0 32-33 0.0 3.999037031882723E-5 0.0 0.10201543468332826 0.0 34-35 0.0 7.998074063765445E-5 0.0 0.17543775458869504 0.0 36-37 0.0 7.998074063765445E-5 0.0 0.2678954907658236 0.0 38-39 0.0 7.998074063765445E-5 0.0 0.418019340942701 0.0 40-41 0.0 7.998074063765445E-5 0.0 0.634207282886281 0.0 42-43 0.0 7.998074063765445E-5 0.0 1.032591352002438 0.0 44-45 0.0 7.998074063765445E-5 0.0 1.5787398394466612 0.0 46-47 0.0 7.998074063765445E-5 0.0 2.135925669098881 0.0 48-49 0.0 7.998074063765445E-5 0.0 2.4112993591143255 0.0 50-51 0.0 7.998074063765445E-5 0.0 2.746978527570561 0.0 52-53 0.0 7.998074063765445E-5 0.0 3.019272959071456 0.0 54-55 0.0 7.998074063765445E-5 0.0 3.2959663313074214 0.0 56-57 0.0 7.998074063765445E-5 0.0 3.7070273478146465 0.0 58-59 0.0 7.998074063765445E-5 0.0 4.181033207203705 0.0 60-61 0.0 7.998074063765445E-5 0.0 4.8201992960095215 3.999037031882723E-5 62-63 0.0 7.998074063765445E-5 0.0 5.548503920256002 7.998074063765445E-5 64-65 0.0 7.998074063765445E-5 0.0 6.193868516461237 7.998074063765445E-5 66-67 0.0 7.998074063765445E-5 0.0 6.748894866116239 7.998074063765445E-5 68-69 0.0 7.998074063765445E-5 0.0 7.237537201041989 7.998074063765445E-5 70-71 0.0 7.998074063765445E-5 0.0 7.788364561813515 7.998074063765445E-5 72-73 0.0 7.998074063765445E-5 0.0 8.3112786441025 7.998074063765445E-5 74-75 0.0 7.998074063765445E-5 0.0 8.84554999156203 7.998074063765445E-5 76-77 0.0 7.998074063765445E-5 0.0 9.447005161157193 7.998074063765445E-5 78-79 0.0 7.998074063765445E-5 0.0 10.193145490565872 7.998074063765445E-5 80-81 0.0 7.998074063765445E-5 0.0 12.002109891938023 7.998074063765445E-5 82-83 0.0 7.998074063765445E-5 0.0 13.304756214703499 7.998074063765445E-5 84-85 0.0 7.998074063765445E-5 0.0 13.937683805739578 7.998074063765445E-5 86-87 0.0 7.998074063765445E-5 0.0 14.486911551698352 7.998074063765445E-5 88-89 0.0 7.998074063765445E-5 0.0 15.118679421995184 7.998074063765445E-5 90-91 0.0 7.998074063765445E-5 0.0 15.899011518026459 7.998074063765445E-5 92-93 0.0 7.998074063765445E-5 0.0 16.836505769410728 7.998074063765445E-5 94-95 0.0 7.998074063765445E-5 0.0 17.90064952359472 7.998074063765445E-5 96-97 0.0 7.998074063765445E-5 0.0 19.001024553287568 7.998074063765445E-5 98-99 0.0 7.998074063765445E-5 0.0 20.10951762815514 7.998074063765445E-5 100-101 0.0 1.1997111095648168E-4 0.0 21.201294728229442 7.998074063765445E-5 102-103 0.0 1.599614812753089E-4 0.0 22.221409084692404 7.998074063765445E-5 104-105 0.0 1.599614812753089E-4 0.0 23.224007658955724 7.998074063765445E-5 106-107 0.0 1.599614812753089E-4 0.0 24.214889054715627 7.998074063765445E-5 108-109 0.0 1.599614812753089E-4 0.0 25.08391979211406 7.998074063765445E-5 110-111 0.0 1.599614812753089E-4 0.0 26.040329488659133 7.998074063765445E-5 112-113 0.0 1.599614812753089E-4 0.0 28.718084685207803 7.998074063765445E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 2145 0.0 83.614655 1 GAGTCCG 635 0.0 79.7606 1 GAGTCGT 640 0.0 79.13747 1 AGTAGTA 1900 0.0 77.96703 2 GAGTCAG 5175 0.0 76.45413 1 AGTCGTA 425 0.0 75.591 2 GAGTACG 585 0.0 75.373566 1 GAGTTAG 1085 0.0 75.23744 1 GAGTAAG 1000 0.0 73.88647 1 GTATACG 165 0.0 72.22529 1 GAGTCGG 650 0.0 70.58633 1 AGTCCGT 265 0.0 69.59549 2 AGTCGAC 165 0.0 68.507 2 GAGTCGA 415 0.0 67.48278 1 AGTACGG 355 0.0 67.03427 2 GTCGACG 170 0.0 66.489426 3 GTGGGGT 5830 0.0 66.32736 7 AGTTTCG 145 0.0 65.64736 2 TGGGGTA 6215 0.0 65.37738 8 GAGTTAC 690 0.0 64.76724 1 >>END_MODULE