##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935962_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1250301 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53821119874334 33.0 31.0 34.0 31.0 34.0 2 32.61855105290646 34.0 31.0 34.0 31.0 34.0 3 32.802658719780275 34.0 31.0 34.0 31.0 34.0 4 36.09623842578707 37.0 35.0 37.0 35.0 37.0 5 36.105033907834994 37.0 35.0 37.0 35.0 37.0 6 36.20394528997417 37.0 37.0 37.0 35.0 37.0 7 36.230371726488265 37.0 37.0 37.0 35.0 37.0 8 36.227905120447 37.0 37.0 37.0 35.0 37.0 9 38.04543146010441 39.0 39.0 39.0 35.0 39.0 10-11 37.836843288136215 39.0 38.0 39.0 35.0 39.0 12-13 37.728606551542384 39.0 38.0 39.0 35.0 39.0 14-15 39.251864950919824 41.0 39.0 41.0 35.5 41.0 16-17 39.30578076799107 41.0 39.0 41.0 35.5 41.0 18-19 39.30132903996717 41.0 39.0 41.0 35.0 41.0 20-21 39.285568035217125 41.0 39.0 41.0 35.0 41.0 22-23 39.128568640671325 41.0 39.0 41.0 35.0 41.0 24-25 38.9331081075677 41.0 38.0 41.0 35.0 41.0 26-27 38.82845770738406 40.5 38.0 41.0 35.0 41.0 28-29 38.715298156203986 40.0 38.0 41.0 35.0 41.0 30-31 38.593624655183035 40.0 38.0 41.0 35.0 41.0 32-33 38.4302983841491 40.0 38.0 41.0 34.5 41.0 34-35 38.08082213802916 40.0 37.0 41.0 34.0 41.0 36-37 37.87806896099419 40.0 37.0 41.0 33.5 41.0 38-39 37.48047750101776 40.0 37.0 41.0 33.0 41.0 40-41 37.2217482030327 40.0 37.0 41.0 33.0 41.0 42-43 37.049028593914585 40.0 37.0 41.0 32.0 41.0 44-45 36.895814687823176 40.0 36.0 41.0 32.0 41.0 46-47 36.7164542778099 40.0 35.5 41.0 31.5 41.0 48-49 36.49398944734108 40.0 35.0 41.0 31.0 41.0 50-51 36.23422959751292 40.0 35.0 41.0 30.5 41.0 52-53 35.97775495660645 39.0 35.0 41.0 30.0 41.0 54-55 35.734019648068745 39.0 35.0 41.0 30.0 41.0 56-57 35.4968759522707 38.5 35.0 41.0 29.5 41.0 58-59 35.19724650304207 37.5 35.0 40.5 29.0 41.0 60-61 34.89644653567421 37.0 35.0 40.0 29.0 41.0 62-63 34.52681074397285 36.5 35.0 39.5 28.0 41.0 64-65 34.13312634317656 36.0 35.0 39.0 28.0 41.0 66-67 33.71745243745306 35.0 34.0 38.5 27.5 41.0 68-69 33.36999890426385 35.0 34.0 37.0 27.0 40.0 70-71 33.137768025459465 35.0 34.0 37.0 27.0 39.0 72-73 32.841052274612274 35.0 34.0 36.0 26.0 39.0 74-75 32.54200348556068 35.0 34.0 36.0 26.0 38.5 76-77 32.35259789442702 35.0 34.0 35.0 26.0 37.0 78-79 32.323279354331476 35.0 34.0 35.0 27.0 37.0 80-81 32.20734247193276 35.0 34.0 35.0 27.0 36.0 82-83 32.07470721050371 35.0 34.0 35.0 26.5 36.0 84-85 31.90325129708766 35.0 34.0 35.0 25.5 36.0 86-87 31.792480770630434 35.0 33.5 35.0 25.0 35.5 88-89 31.730082596110854 35.0 34.0 35.0 25.0 35.5 90-91 31.71196095980088 35.0 34.0 35.0 25.0 35.0 92-93 31.684438387236355 35.0 34.0 35.0 25.0 36.0 94-95 31.594294893789574 35.0 33.5 35.0 25.0 35.0 96-97 31.55368627234562 35.0 33.5 35.0 25.0 35.0 98-99 31.471452074340498 35.0 33.0 35.0 25.0 35.0 100-101 31.433647977567002 35.0 33.0 35.0 24.0 35.0 102-103 30.744521919121876 34.5 32.0 35.0 23.0 35.0 104-105 31.281113907771008 35.0 33.0 35.0 24.0 35.0 106-107 31.368153348673637 35.0 33.0 35.0 24.0 35.0 108-109 31.312106444768098 35.0 33.0 35.0 23.5 35.0 110-111 31.226436274145186 35.0 33.0 35.0 23.0 35.0 112-113 31.177941151770654 35.0 33.0 35.0 23.0 35.0 114-115 31.069064969155427 35.0 33.0 35.0 21.0 35.0 116-117 30.93758143039156 35.0 33.0 35.0 19.0 35.0 118-119 30.750301327440354 35.0 33.0 35.0 12.0 35.0 120-121 30.461561256049542 35.0 33.0 35.0 2.0 35.0 122-123 30.1292300813964 35.0 33.0 35.0 2.0 35.0 124-125 27.88880197648406 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08464534245116795 1101 2 0.17240151036453 1101 3 0.12120867992370421 1101 4 0.1678542967025436 1101 5 0.24193462557692413 1101 6 0.23397769836936533 1101 7 0.19125948567634765 1101 8 0.17180576784912205 1101 9 0.1712634823664274 1101 10-11 0.20410856029622693 1101 12-13 0.21751302963973984 1101 14-15 0.31496908404933066 1101 16-17 0.21891324594819395 1101 18-19 0.22464467443668923 1101 20-21 0.12614186748659506 1101 22-23 0.23153522937521132 1101 24-25 0.3735747639981142 1101 26-27 0.3634430618703277 1101 28-29 0.404749404276906 1101 30-31 0.39567162487772123 1101 32-33 0.4444872349098574 1101 34-35 0.4823581418515346 1101 36-37 0.43203436289071107 1101 38-39 0.5853847950900786 1101 40-41 0.5720607133530891 1101 42-43 0.5060574252837426 1101 44-45 0.6504751927792682 1101 46-47 0.693426889474722 1101 48-49 0.6254053429166504 1101 50-51 0.6231349214979147 1101 52-53 0.6668028253378893 1101 54-55 0.8459691761004393 1101 56-57 0.7013026915744476 1101 58-59 0.590953731180214 1101 60-61 0.49431887803526564 1101 62-63 0.5361718292280955 1101 64-65 0.48214542841677144 1101 66-67 0.35860325806782356 1101 68-69 0.5397389999993791 1101 70-71 0.5104441784210536 1101 72-73 0.42591171634168035 1101 74-75 0.4672861975908873 1101 76-77 0.4844724379163452 1101 78-79 0.4878474179164627 1101 80-81 0.5950480371178983 1101 82-83 0.5220806099689526 1101 84-85 0.4833074608392529 1101 86-87 0.4815716675552686 1101 88-89 0.4054404504287987 1101 90-91 0.367187083234672 1101 92-93 0.3743296169069481 1101 94-95 0.4756460885700555 1101 96-97 0.48274660683337345 1101 98-99 0.4863414400085162 1101 100-101 0.3876724576770805 1101 102-103 0.5264363140078032 1101 104-105 0.5558901355033576 1101 106-107 0.4665843742920295 1101 108-109 0.49863515317925433 1101 110-111 0.45774600569016854 1101 112-113 0.5347062057886589 1101 114-115 0.5566209980742407 1101 116-117 0.43975533144297785 1101 118-119 0.178287164997883 1101 120-121 0.37651762522418153 1101 122-123 0.41454867099588455 1101 124-125 0.41581214479699824 1102 1 -0.10409514718006108 1102 2 -0.03473234376352252 1102 3 0.06479122302478402 1102 4 0.12433337344781137 1102 5 0.1438881201529938 1102 6 0.1317483512169133 1102 7 0.16728627913492033 1102 8 0.08258257168409955 1102 9 0.1075981551125409 1102 10-11 0.15169337804587002 1102 12-13 0.17845768281436136 1102 14-15 0.20558268220491982 1102 16-17 0.2003444582273275 1102 18-19 0.12496082646352136 1102 20-21 0.15665522075917693 1102 22-23 0.18188478099061456 1102 24-25 0.22525167876300145 1102 26-27 0.20429765854413517 1102 28-29 0.2447091473962857 1102 30-31 0.19963572856791956 1102 32-33 0.2627365359662548 1102 34-35 0.36098460461338533 1102 36-37 0.3390272398918839 1102 38-39 0.2020786650683064 1102 40-41 0.16379819081324598 1102 42-43 0.03278702909686615 1102 44-45 0.08747892154267589 1102 46-47 0.17758576438218654 1102 48-49 0.07873478347442386 1102 50-51 -0.015620591740066914 1102 52-53 0.018795821179871552 1102 54-55 0.16684148892350947 1102 56-57 0.17122372806495179 1102 58-59 0.12594986619330228 1102 60-61 0.05849752663434771 1102 62-63 0.09383126126835606 1102 64-65 0.17141520308395286 1102 66-67 0.0894369848765777 1102 68-69 0.1351981458993592 1102 70-71 0.06332514569300685 1102 72-73 0.07529922500198438 1102 74-75 0.12479549998824524 1102 76-77 0.12697569362239136 1102 78-79 0.11004876120782825 1102 80-81 0.1171277725605151 1102 82-83 0.07127556104379096 1102 84-85 0.09640577553468788 1102 86-87 0.1248837502599578 1102 88-89 0.0738293628296347 1102 90-91 0.0015325382122703957 1102 92-93 0.014908006255687667 1102 94-95 0.07107206546811184 1102 96-97 0.12957495849007117 1102 98-99 0.10079205668588997 1102 100-101 0.046697955948211245 1102 102-103 0.013855329317692622 1102 104-105 -0.1008165479973151 1102 106-107 0.015998560053340327 1102 108-109 -0.17891177386348645 1102 110-111 -0.09039055268709717 1102 112-113 -0.11546458796507153 1102 114-115 -0.11854822591656244 1102 116-117 0.07298137694943208 1102 118-119 0.11744687888597483 1102 120-121 0.29005153582822274 1102 122-123 0.18171575798061568 1102 124-125 0.11131112897414397 1103 1 -0.047313873929425654 1103 2 0.043091564244100766 1103 3 0.10098782403777307 1103 4 0.12554785222330622 1103 5 0.10270661738073272 1103 6 0.17170220372528888 1103 7 0.15705878726662803 1103 8 0.1297912124506695 1103 9 0.16737813721957906 1103 10-11 0.21112212046255507 1103 12-13 0.12490933450212083 1103 14-15 0.1928083648510821 1103 16-17 0.24852525615609267 1103 18-19 0.2159474418672147 1103 20-21 0.15046698532995606 1103 22-23 0.25403312842475856 1103 24-25 0.25215832769657 1103 26-27 0.21458408437970178 1103 28-29 0.3318206433700368 1103 30-31 0.37840237741367844 1103 32-33 0.35057432131895894 1103 34-35 0.41376917546434555 1103 36-37 0.44190720271452477 1103 38-39 0.4743926017526192 1103 40-41 0.3157490402222649 1103 42-43 0.40165945850359464 1103 44-45 0.36901121144646254 1103 46-47 0.419294565739591 1103 48-49 0.40440608188935556 1103 50-51 0.39413748854590125 1103 52-53 0.4028225545730706 1103 54-55 0.38685026316115056 1103 56-57 0.4061728540353826 1103 58-59 0.42983130118305723 1103 60-61 0.4845195243708744 1103 62-63 0.47149869874853323 1103 64-65 0.40658469818703225 1103 66-67 0.3594253095341742 1103 68-69 0.3970919154813899 1103 70-71 0.42105297613609594 1103 72-73 0.3923023331355111 1103 74-75 0.4158691823074747 1103 76-77 0.42530939902213305 1103 78-79 0.45372328900153036 1103 80-81 0.3702787134269485 1103 82-83 0.4549248806350974 1103 84-85 0.36030481979122086 1103 86-87 0.40029957245972625 1103 88-89 0.27871845045415 1103 90-91 0.39895383097021764 1103 92-93 0.40317781642751527 1103 94-95 0.2604996832423261 1103 96-97 0.35682062127092706 1103 98-99 0.3773328590862093 1103 100-101 0.4004893688807911 1103 102-103 0.3823983026494808 1103 104-105 0.3029098349329722 1103 106-107 0.3439161038341858 1103 108-109 0.2621637153137719 1103 110-111 0.3053209795588998 1103 112-113 0.3015332515731046 1103 114-115 0.2843542635285168 1103 116-117 0.29283834134014697 1103 118-119 0.3411465405813594 1103 120-121 0.3786595155211643 1103 122-123 0.19808859350824193 1103 124-125 0.2595075975967909 1104 1 0.02625239871748164 1104 2 0.0726932671202789 1104 3 0.17426661399641574 1104 4 0.12309797175558401 1104 5 0.17247670205452437 1104 6 0.09504798713010132 1104 7 0.12757008150531846 1104 8 0.0815958504188572 1104 9 0.1108206667581868 1104 10-11 0.2276230794893479 1104 12-13 0.1953982868611206 1104 14-15 0.26519814759399907 1104 16-17 0.21016933520385095 1104 18-19 0.17405287622361243 1104 20-21 0.14340498757960063 1104 22-23 0.19423479769154284 1104 24-25 0.2743255608511461 1104 26-27 0.2506848919710549 1104 28-29 0.22257625604296294 1104 30-31 0.2836567110622852 1104 32-33 0.22809945271431786 1104 34-35 0.3263673557922928 1104 36-37 0.31189790540204143 1104 38-39 0.3092254271891406 1104 40-41 0.3286419788361812 1104 42-43 0.21209778429198423 1104 44-45 0.3431105664936993 1104 46-47 0.3694005448000297 1104 48-49 0.38966382154786317 1104 50-51 0.368801346273834 1104 52-53 0.36335855365107506 1104 54-55 0.3771527928156502 1104 56-57 0.2881850332571787 1104 58-59 0.31722194991527175 1104 60-61 0.22955215832396902 1104 62-63 0.2889133891461029 1104 64-65 0.35441866260870825 1104 66-67 0.47805698851677647 1104 68-69 0.5093601828654002 1104 70-71 0.4732899970814515 1104 72-73 0.4521642448664167 1104 74-75 0.4669282646678852 1104 76-77 0.443184190492552 1104 78-79 0.46768921731307245 1104 80-81 0.4643298177403494 1104 82-83 0.46640809171049114 1104 84-85 0.43922557823043107 1104 86-87 0.4544309852069581 1104 88-89 0.523271731974063 1104 90-91 0.46297023776498136 1104 92-93 0.4830254087519563 1104 94-95 0.4856538389929135 1104 96-97 0.45166528082123847 1104 98-99 0.4506370814439258 1104 100-101 0.4416253085950572 1104 102-103 0.43728125888579683 1104 104-105 0.44958524090166563 1104 106-107 0.4325594430888664 1104 108-109 0.4583704890883986 1104 110-111 0.46616190988249784 1104 112-113 0.5964439709751908 1104 114-115 0.6573680414502334 1104 116-117 0.6454820553203007 1104 118-119 0.6096626148233604 1104 120-121 0.5565741167443647 1104 122-123 0.5209915312400675 1104 124-125 0.4511724346330581 1105 1 0.10178448778609805 1105 2 0.10802818955657045 1105 3 0.10552037639170209 1105 4 0.1239209519523925 1105 5 0.1829728121969154 1105 6 0.13225546965111334 1105 7 0.11603466316431366 1105 8 0.20921131821337724 1105 9 0.15086126794459886 1105 10-11 0.13077645697642737 1105 12-13 0.18562150623265694 1105 14-15 0.24189650916618888 1105 16-17 0.3329944606273969 1105 18-19 0.3188510126279098 1105 20-21 0.31917802557800456 1105 22-23 0.30676800170534335 1105 24-25 0.2391815984567316 1105 26-27 0.3031508133073473 1105 28-29 0.3468982467337014 1105 30-31 0.24686974527572403 1105 32-33 0.3227077807509886 1105 34-35 0.26647967204577583 1105 36-37 0.31737380521759206 1105 38-39 0.17951842556994535 1105 40-41 0.1348815009349451 1105 42-43 0.08733769048967588 1105 44-45 0.16750436563051352 1105 46-47 0.08176258511858947 1105 48-49 -0.03915539485296904 1105 50-51 -0.05125129457748301 1105 52-53 -0.01634409028175554 1105 54-55 -0.07447782229667865 1105 56-57 -0.13756045793811467 1105 58-59 -0.15969740860840176 1105 60-61 -0.19416451860401196 1105 62-63 -0.18469366084340777 1105 64-65 -0.1361145750215229 1105 66-67 -0.09858117473087447 1105 68-69 -0.15185211303145962 1105 70-71 -0.1543651567853388 1105 72-73 -0.13196478124670818 1105 74-75 -0.1248786935612003 1105 76-77 -0.05537403784646955 1105 78-79 -0.07324875600170344 1105 80-81 -0.0659011645381824 1105 82-83 -0.1125787088575052 1105 84-85 -0.022492925190661595 1105 86-87 0.011690050284023812 1105 88-89 -0.06171918224985973 1105 90-91 -0.044425845422008337 1105 92-93 0.059756639729876326 1105 94-95 0.004465504145802157 1105 96-97 0.017379658135872944 1105 98-99 -0.05136371912531246 1105 100-101 -0.01874791479177418 1105 102-103 -0.040182499170477115 1105 104-105 0.034937064935800066 1105 106-107 -0.013064256997726176 1105 108-109 -0.005998265802325875 1105 110-111 0.024342625626260883 1105 112-113 0.06583106861288712 1105 114-115 0.16829027106668804 1105 116-117 0.17151207230905285 1105 118-119 0.2165060811263082 1105 120-121 0.21319076547189297 1105 122-123 0.2719077347988055 1105 124-125 0.2617716663106968 1106 1 0.032004811350439866 1106 2 0.1318478611812708 1106 3 0.16769758963769021 1106 4 0.1636144059517406 1106 5 0.14591309115485274 1106 6 0.238428410616919 1106 7 0.14323910604776557 1106 8 0.23774597470183068 1106 9 0.22122491865059857 1106 10-11 0.21451212868630165 1106 12-13 0.2362209121805563 1106 14-15 0.20345363591679444 1106 16-17 0.25013340061362754 1106 18-19 0.22452297145517264 1106 20-21 0.32933908790128896 1106 22-23 0.3471913485587592 1106 24-25 0.33675839017347897 1106 26-27 0.38858816864473766 1106 28-29 0.3858447115598267 1106 30-31 0.39778945226281337 1106 32-33 0.4752622857660569 1106 34-35 0.5448870809306428 1106 36-37 0.5566301933172326 1106 38-39 0.5382026508562419 1106 40-41 0.6183961502309501 1106 42-43 0.517028777998533 1106 44-45 0.49929728061591305 1106 46-47 0.5639683162246882 1106 48-49 0.5159298452948278 1106 50-51 0.5346053704825309 1106 52-53 0.6104998002286166 1106 54-55 0.6193609942061258 1106 56-57 0.6083447486708948 1106 58-59 0.5473192207113087 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-0.9879649889996962 2216 120-121 -1.257970434235773 2216 122-123 -1.2337298522420816 2216 124-125 -1.539443256901695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 27.0 4 69.0 5 142.0 6 407.0 7 863.0 8 1188.0 9 1935.0 10 4529.0 11 14571.0 12 20401.0 13 7007.0 14 6099.0 15 7994.0 16 4990.0 17 3121.0 18 2785.0 19 2574.0 20 2575.0 21 2597.0 22 2898.0 23 3073.0 24 4032.0 25 5200.0 26 6991.0 27 8951.0 28 11606.0 29 14749.0 30 19214.0 31 24719.0 32 32584.0 33 44711.0 34 78684.0 35 124181.0 36 247357.0 37 444402.0 38 92670.0 39 402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.64551655591981 11.75289959041031 8.012969004240174 15.5886148494297 2 12.698992878486374 28.154533006584042 43.4995393090916 15.646934805837978 3 33.66221413883537 20.62375380008494 21.391169006503233 24.32286305457646 4 14.99173216208966 16.243799121567246 44.02789844772845 24.736570268614646 5 16.84526762140971 33.27650212016962 29.47211776942155 20.40611248899912 6 31.674816880139872 37.42491645831234 15.530835640827531 15.36943102072026 7 41.741388673607396 24.184896276976502 18.214014065413046 15.859700984003053 8 40.27902081178853 24.591518362378338 17.170345380832295 17.959115445000844 9 47.45161365143274 10.695344561029705 16.107161395535954 25.74588039200161 10-11 34.049040990929385 24.22596638729394 24.7114494829645 17.013543138812175 12-13 28.46858476478864 25.4793045834563 27.672936356925256 18.379174294829806 14-15 24.45343161366743 25.395324805786768 26.80770470470711 23.3435388758387 16-17 22.924239843045793 30.78490699439575 25.42999645685319 20.860856705705267 18-19 23.68305711984554 24.664060894136693 27.89320331664135 23.759678669376413 20-21 23.962829750596057 24.905042865677945 26.682214922646626 24.449912461079375 22-23 24.1407069177742 23.22448754339955 26.886685686086793 25.74811985273946 24-25 20.862256368666426 22.443675562924447 35.550799367512305 21.143268700896826 26-27 18.825186894995685 22.781274269155986 32.986656813039424 25.406882022808908 28-29 21.677859971318906 23.034653255496078 27.011935525925356 28.27555124725966 30-31 21.935757869505025 26.347215590485813 26.692492447818566 25.0245340921906 32-33 28.33357727459228 22.644947096739106 25.03809082772868 23.983384800939934 34-35 26.712287681126384 18.797753500956972 30.032248234625104 24.457710583291544 36-37 24.22324704211226 24.341378596034076 30.67813270564448 20.757241656209185 38-39 23.07340392433502 27.749277973863894 26.438913509626882 22.738404592174206 40-41 21.334782584353686 26.338577670496942 28.544006603209947 23.78263314193942 42-43 22.499462129519213 22.248802488360802 25.88384716960156 29.367888212518427 44-45 22.83826054686032 23.55192869557011 25.579080557401777 28.030730200167795 46-47 23.576362811834912 22.762318833624864 25.349295889549794 28.312022464990434 48-49 19.174782712322873 22.912162751209507 26.61183187088549 31.30122266558213 50-51 22.86885318015422 24.12850985482696 26.67597642487689 26.326660540141933 52-53 23.38376918837944 22.53061462799758 29.588315133715803 24.497301049907183 54-55 21.082963222456033 24.202252097694878 28.864569411685665 25.850215268163428 56-57 21.624152903980722 25.818542894870916 27.47526395643929 25.082040244709074 58-59 24.047009480117186 22.583601868670026 30.222562406972404 23.146826244240387 60-61 24.988502768533337 27.401481723201055 27.458827914238253 20.15118759402736 62-63 25.496100538990213 23.85057678111111 26.638145534555278 24.0151771453434 64-65 28.61291001126929 20.438998289211955 28.03720864015945 22.910883059359303 66-67 28.353772411603284 24.332540724193613 24.683416233371005 22.630270630832094 68-69 22.624831940468734 27.547086661531907 25.837138417069173 23.990942980930193 70-71 24.31490497088301 26.320222090520602 24.419919683340254 24.944953255256134 72-73 23.42971812387577 27.017614158510632 24.857894219072048 24.69477349854155 74-75 21.32990375917479 26.447231506653196 27.10323354136324 25.11963119280877 76-77 22.700293769260362 27.64846225029013 24.082240996368075 25.569002984081436 78-79 22.9369967711775 28.174215648871755 21.598119172903164 27.29066840704758 80-81 22.52137685245393 23.288352164798717 25.077601313603687 29.11266966914367 82-83 24.489582908435647 20.359057538944622 27.212767165666506 27.93859238695322 84-85 25.305346472569408 21.459072655304603 29.260634039323328 23.97494683280266 86-87 26.1778963625559 23.929837695083023 24.69033456743616 25.201931374924918 88-89 22.60103767012903 25.639665968434798 26.688333449305407 25.070962912130756 90-91 20.599999520115556 26.716446679639542 26.102754456726817 26.58079934351808 92-93 24.23992302653521 22.980906197787572 26.303026231283503 26.47614454439371 94-95 22.299510277925076 22.601677516054135 28.29194729909038 26.80686490693041 96-97 22.103477482622186 21.964470955393942 27.939432184729917 27.99261937725396 98-99 20.224210010229537 23.724127230162978 28.97670241005966 27.074960349547826 100-101 20.22181058801041 26.53309083172772 27.028491539237354 26.21660704102452 102-103 21.70537334609826 25.925557125844094 25.89664408810358 26.472425439954062 104-105 20.91980251155522 23.28711246331883 24.91436062196223 30.87872440316372 106-107 20.541061928712338 25.06073539921003 23.90117415773248 30.497028514345153 108-109 22.557728099073742 24.45199196033595 25.71704733500173 27.273232605588575 110-111 23.57865690891095 26.672025434150143 23.513631864671126 26.235685792267777 112-113 20.80450517137335 26.773169404217317 24.88577709367338 27.53654833073595 114-115 21.24232380544096 24.456247890496154 26.207955888774958 28.093472415287923 116-117 23.69467905523626 24.0056597201254 25.10628971992144 27.1933715047169 118-119 21.591618480581666 24.313460764016224 25.791456196219308 28.303464559182807 120-121 20.029177090864884 24.833639394191458 27.06727047035262 28.06991304459104 122-123 19.932998659893215 26.66224630050945 28.089853939957937 25.314901099639396 124-125 20.29683860445934 27.15445599923516 24.89518484309428 27.65352055321122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 8.5 2 58.0 3 157.5 4 256.5 5 349.0 6 433.5 7 541.5 8 706.5 9 924.5 10 1119.5 11 1315.5 12 1539.5 13 1726.0 14 1905.5 15 2055.5 16 2175.0 17 2263.0 18 2398.5 19 2498.5 20 2561.0 21 2692.0 22 2927.5 23 3109.0 24 3177.0 25 3419.0 26 3717.0 27 3933.5 28 4177.0 29 4551.5 30 5219.0 31 5842.0 32 6434.5 33 7304.0 34 8381.0 35 9317.5 36 10158.0 37 11213.0 38 12108.5 39 12777.5 40 13350.0 41 14521.5 42 15901.5 43 17342.0 44 19273.5 45 23090.5 46 37830.5 47 67569.0 48 94581.5 49 112005.5 50 113549.5 51 94594.5 52 72076.0 53 55782.0 54 46959.0 55 42323.0 56 38021.0 57 34025.5 58 31347.5 59 27243.5 60 23759.0 61 20525.5 62 16048.0 63 12542.5 64 10191.0 65 9169.5 66 8163.0 67 7267.0 68 6204.0 69 4692.5 70 3415.0 71 2441.0 72 1941.5 73 1629.5 74 1291.0 75 1068.5 76 758.5 77 521.5 78 433.5 79 351.0 80 342.5 81 336.5 82 229.0 83 90.5 84 21.5 85 14.0 86 9.0 87 5.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21682778786868123 2 3.999037031882723E-4 3 0.0 4 0.11885138058755451 5 0.032072276995699436 6 0.00215947999721667 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 3.999037031882723E-5 108-109 0.0 110-111 0.001079739998608335 112-113 3.59913332869445E-4 114-115 5.598651844635811E-4 116-117 0.004998796289853403 118-119 0.002119489626897843 120-121 3.999037031882723E-4 122-123 0.0021994703675354975 124-125 0.02899301848114974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1250301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.46125628896339 #Duplication Level Percentage of deduplicated Percentage of total 1 81.17858538431652 36.90480475331911 2 10.321225949435778 9.38431796207199 3 3.0264252657197193 4.127552839328349 4 1.4330828912672682 2.6059899441291976 5 0.806721804720467 1.8337293359146116 6 0.5395494212009493 1.4717156710666928 7 0.3990139797304527 1.2697773756783748 8 0.3011614955555189 1.0952943947053562 9 0.23360863030434717 0.9558127632221666 >10 1.4770333551591488 12.826463706723157 >50 0.1513928740202893 4.79062225279407 >100 0.11549828446730835 10.48988553201054 >500 0.010020398461411596 3.033496154722204 >1k 0.005449690391294026 4.368228069257482 >5k 8.789823211764559E-4 3.103265687168722 >10k+ 3.5159292847058234E-4 1.7390435578880088 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11307 0.9043422343899589 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10453 0.836038681885402 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 9949 0.7957283886040242 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 9188 0.7348630449787691 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8214 0.6569618035976937 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6144 0.4914016704777489 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5335 0.4266972513018865 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3528 0.2821720529696449 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3159 0.25265915967435043 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3109 0.2486601226424677 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2945 0.2355432811778924 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2682 0.21450834639018926 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2334 0.1866750486482855 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2324 0.18587524124190896 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2127 0.17011903533629102 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1930 0.1543628294306731 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1834 0.14668467832945825 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1787 0.1429255835194885 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1712 0.13692702797166442 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1698 0.13580729760273724 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1675 0.1339677405680712 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1646 0.13164829908957923 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1587 0.1269294353919576 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1534 0.12269045613816194 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1478 0.11821153466245328 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1478 0.11821153466245328 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1470 0.11757168873735205 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1340 0.10717419245445696 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1300 0.10397496282895079 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1264 0.10109565616599522 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.998074063765445E-5 0.0 20-21 0.0 7.998074063765445E-5 0.0 5.998555547824084E-4 0.0 22-23 0.0 7.998074063765445E-5 0.0 0.0019995185159413613 0.0 24-25 0.0 7.998074063765445E-5 0.0 0.003919056291245068 0.0 26-27 0.0 7.998074063765445E-5 0.0 0.006518430361968837 0.0 28-29 0.0 7.998074063765445E-5 0.0 0.014316552574140148 0.0 30-31 0.0 7.998074063765445E-5 0.0 0.042349802167638034 0.0 32-33 0.0 7.998074063765445E-5 0.0 0.10217539616460357 0.0 34-35 0.0 7.998074063765445E-5 0.0 0.1756377064402892 0.0 36-37 0.0 7.998074063765445E-5 0.0 0.26813543298773657 0.0 38-39 0.0 7.998074063765445E-5 0.0 0.4183392639052516 0.0 40-41 0.0 7.998074063765445E-5 0.0 0.6350070902926575 0.0 42-43 0.0 1.1997111095648168E-4 0.0 1.034190966815191 0.0 44-45 0.0 1.599614812753089E-4 0.0 1.582019049812805 0.0 46-47 0.0 1.599614812753089E-4 0.0 2.140084667612039 0.0 48-49 0.0 1.599614812753089E-4 0.0 2.4166180783667293 0.0 50-51 0.0 1.599614812753089E-4 0.0 2.753976842376356 0.0 52-53 0.0 1.599614812753089E-4 0.0 3.0287906672073364 0.0 54-55 0.0 1.599614812753089E-4 0.0 3.3076435194405187 0.0 56-57 0.0 1.599614812753089E-4 0.0 3.720983987055917 0.0 58-59 0.0 1.599614812753089E-4 0.0 4.197389268664105 0.0 60-61 0.0 1.599614812753089E-4 0.0 4.838434904874907 0.0 62-63 0.0 1.599614812753089E-4 0.0 5.56801922097159 0.0 64-65 0.0 1.599614812753089E-4 0.0 6.213783720880013 0.0 66-67 0.0 1.599614812753089E-4 0.0 6.770289714236812 0.0 68-69 0.0 1.599614812753089E-4 0.0 7.261091529159778 0.0 70-71 0.0 1.599614812753089E-4 0.0 7.814918167705216 0.0 72-73 0.0 1.599614812753089E-4 0.0 8.339351884066318 0.0 74-75 0.0 1.599614812753089E-4 0.0 8.876022653744979 0.0 76-77 0.0 1.599614812753089E-4 0.0 9.480117187781183 0.0 78-79 0.0 1.599614812753089E-4 0.0 10.231576236442265 0.0 80-81 0.0 1.599614812753089E-4 0.0 12.048058827434353 0.0 82-83 0.0 1.599614812753089E-4 0.0 13.358703224263596 0.0 84-85 0.0 1.599614812753089E-4 0.0 13.99522994862837 0.0 86-87 0.0 1.599614812753089E-4 0.0 14.549136567914445 0.0 88-89 0.0 1.599614812753089E-4 0.0 15.185623301908901 0.0 90-91 0.0 1.599614812753089E-4 0.0 15.970794232748755 0.0 92-93 0.0 1.599614812753089E-4 0.0 16.914207058940207 0.0 94-95 0.0 1.599614812753089E-4 0.0 17.98718868496466 0.0 96-97 0.0 1.599614812753089E-4 0.0 19.096881470941796 0.0 98-99 0.0 1.599614812753089E-4 0.0 20.213412610243452 0.0 100-101 0.0 1.599614812753089E-4 0.0 21.312827871048654 0.0 102-103 0.0 1.599614812753089E-4 0.0 22.34261989712877 0.0 104-105 0.0 1.599614812753089E-4 0.0 23.35373642027 0.0 106-107 0.0 1.599614812753089E-4 0.0 24.353655639721957 0.0 108-109 0.0 1.599614812753089E-4 0.0 25.23096438377639 0.0 110-111 0.0 1.599614812753089E-4 0.0 26.197531634382443 0.0 112-113 0.0 1.599614812753089E-4 0.0 28.88968336424589 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 500 0.0 81.200264 1 AGTAGTA 1660 0.0 76.080154 2 GAGTAGT 2025 0.0 75.775116 1 GAGTCGT 665 0.0 73.62253 1 GAGTCGA 335 0.0 71.29083 1 GAGTTCG 380 0.0 70.70456 1 GAGTCAG 4690 0.0 68.999344 1 GATTGTG 7735 0.0 68.610725 9 GAGTCCG 605 0.0 68.094536 1 ACGGGAT 795 0.0 67.37547 5 CGGGATT 2460 0.0 66.99342 6 AGTACGT 125 0.0 66.720856 2 TCGTAGG 1110 0.0 66.54912 2 GAGTCGG 880 0.0 66.49086 1 GTAATCG 145 0.0 65.88257 1 CGGGATA 615 0.0 65.78416 6 GAGTAAG 1065 0.0 64.471344 1 AGTCCGT 260 0.0 64.15467 2 GTATACG 140 0.0 63.9708 1 AGTCGTA 500 0.0 63.146523 2 >>END_MODULE