FastQCFastQC Report
Thu 26 May 2016
SRR935961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475654
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8500.1787013249126466No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT8260.1736556404445248No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7190.15116029719081517No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6990.14695556013404704No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6170.12971613820129757No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5010.10532866327204228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTG751.4602138E-647.6413761
GTGTACG1603.6379788E-1240.9418071
TGTGAGG1508.54925E-1139.6635862
TCTACGG600.001308239239.663582
TGTACGG1655.456968E-1239.663582
TACTTGG957.467015E-637.5760232
TCTTAGG650.00193849836.6125372
GTGTGAG1801.4551915E-1136.3927151
AGTCGGC1001.0622107E-535.6934747
GTTCAGG2550.035.0304221
TAGGGCA1209.286505E-734.701995
GTTAGAG1051.4826523E-533.9973563
TACGGGA2101.8189894E-1233.9973564
GTTAACG700.002788413833.9973563
GTATAGG1251.2672936E-633.348961
TTATAGG902.3919855E-433.0529862
TACGAGG1451.09625034E-732.8250352
ATTATTT552.0419555E-532.448612100-101
AGTCAAT1301.7342991E-632.035972
TATGGGA750.003909689431.7308674
Produced by FastQC (version 0.11.5)