FastQCFastQC Report
Thu 26 May 2016
SRR935961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475654
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT80441.691145244232152No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT45570.9580493383846242TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT26240.5516615018479819RNA PCR Primer, Index 3 (95% over 23bp)
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7790.16377450836111965No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7470.15704692907029058No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7250.15242171830784562No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7220.1517910077493304No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7140.15010911292662313No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG7010.14737603383972384TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC6930.14569413901701658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA800.074.566491
GTATAGA250.002035236271.583841
TTAGGGT508.844836E-871.485894
GCGTCAG701.70985E-1068.175071
TATCACA300.004192118559.653191
CAGGTAT300.004192118559.653191
CCTAGGG507.392413E-659.5715753
TAGGGTG300.004230747559.515195
GAACGCG150.00423733659.49642690-91
TATGGGC551.3002438E-554.155984
GGTCGTG453.1699767E-452.8857157
GTTATAG751.4431807E-647.7225531
TATTCGG505.2979303E-447.657262
CGTCAGA1109.509677E-943.3247832
GTCGTGC558.525884E-443.270138
AGGTCGT558.525884E-443.270136
GTATTGG2350.043.1533741
GTATTAG705.3651056E-542.609421
TTATAGG853.4479453E-642.0505182
GTGTTAG905.058937E-639.76881
Produced by FastQC (version 0.11.5)