FastQCFastQC Report
Thu 26 May 2016
SRR935958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528330
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC109790.7183657979624819No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT107640.7042981555030654No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT99380.6502522361008422No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG82420.539281437909352No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT75740.49557359994242084No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA61230.4006333710651495No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT50680.3316037766712686No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC38220.25007688130181305No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC33210.21729600282661465No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG28480.1863471894158984No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG27110.1773831567789679No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG25080.16410068506147232No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC23870.15618354674710305No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA22160.14499486367472994No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA21840.14290107502960747No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA18870.12346809916706471No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA18640.12196318857838294No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC18590.12163603410258256No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC17800.11646699338493649No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG16790.10985847297376874No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT15640.10233392003035993No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15370.10056728586103787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT21050.086.625111
AGTAGTA17500.085.008982
GAGTACG6800.075.3640061
GAGTTAG10700.073.513081
GAGTAAG12150.073.077621
GAGTCCG7250.069.042371
GAGTCGG8300.068.923541
GAGTCAG49850.068.854411
AGTCGGT3900.068.66112
TCGTAGG10850.064.7165152
GAGTTAC7050.064.239021
TGGGGTA64150.064.1676258
AGTCCGT2900.063.610162
GTGGGGT63250.062.72957
AGTCGTA4150.060.223232
AGTACGG4850.060.119752
GGGGTAA76950.059.523529
GAGTCGT6050.059.0976641
AGGGTAA67900.057.820346
GATTGTG65150.057.7044339
Produced by FastQC (version 0.11.5)