FastQCFastQC Report
Thu 26 May 2016
SRR935958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528330
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT112120.7336111965347798No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT103150.674919683576191No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC94080.6155738616660015No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG89060.582727552295643No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT77390.5063696976438335No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT56300.36837593975123173No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA50740.3319963620422291No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG31950.209051710036445No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC31550.20643447423004196No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG29250.19138536834322428No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC27250.17829918931120897No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG26740.17496221365804507No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC26510.17345730306936327No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA23530.15395889631166043No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC19150.12530016423154686No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT17920.11725216412685742No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG17750.11613983890913612No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA17330.1133917413124129No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT17140.11214855430437143No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA15860.10377339972388161No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT15650.10239935092552001No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA15360.10050185496587778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT20700.086.575281
AGTAGTA15750.083.261562
GAGTCAG45550.070.556471
GAGTTAG12150.069.3243561
TCGTAGG9900.068.639182
GTATACG1600.067.2040561
GAGTAAG11650.067.172011
GAGTCGA4700.064.820931
GAGTCGG7950.064.621091
GATTGTG68100.062.552339
AGGGTAA64500.061.2491576
GAGTACG5650.060.265591
TGGGGTA57350.059.753958
GAGTCGT6100.059.7369421
GTCGTAG11600.059.221971
GAGTAAA8350.058.6638221
GATAGCG19000.058.5552149
GTAGTAG34250.058.254421
GAGTCCG6100.057.7783551
AGTTAGT7700.057.2853282
Produced by FastQC (version 0.11.5)