##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935957_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833057 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06554533483303 31.0 31.0 34.0 28.0 34.0 2 30.96509122424996 31.0 31.0 34.0 28.0 34.0 3 31.23177885786927 33.0 31.0 34.0 28.0 34.0 4 34.404115204601844 37.0 35.0 37.0 32.0 37.0 5 34.599064649837885 37.0 35.0 37.0 32.0 37.0 6 34.727204741092145 37.0 35.0 37.0 32.0 37.0 7 34.69931589315017 37.0 35.0 37.0 32.0 37.0 8 34.69335111522981 37.0 35.0 37.0 32.0 37.0 9 36.27800858764766 39.0 37.0 39.0 32.0 39.0 10-11 36.0206954626154 39.0 37.0 39.0 31.0 39.0 12-13 35.84442721206352 39.0 37.0 39.0 30.5 39.0 14-15 37.31487761341661 40.0 38.0 41.0 31.0 41.0 16-17 37.500214271052286 40.0 38.0 41.0 31.5 41.0 18-19 37.55371541203063 40.0 38.0 41.0 31.5 41.0 20-21 37.67203084542834 40.0 38.0 41.0 32.0 41.0 22-23 37.589337224223556 40.0 38.0 41.0 31.5 41.0 24-25 37.514480401701206 40.0 38.0 41.0 31.0 41.0 26-27 37.47726446089523 40.0 38.0 41.0 31.0 41.0 28-29 37.35623192650683 40.0 37.0 41.0 31.0 41.0 30-31 37.28171181563806 40.0 37.0 41.0 31.0 41.0 32-33 37.19960098768752 40.0 37.0 41.0 30.5 41.0 34-35 36.93472475472866 40.0 37.0 41.0 30.0 41.0 36-37 36.91930384115373 40.0 36.5 41.0 30.0 41.0 38-39 37.0513320216984 40.0 37.0 41.0 30.0 41.0 40-41 36.99982894327759 40.0 36.0 41.0 30.0 41.0 42-43 36.812844139116535 40.0 36.0 41.0 30.0 41.0 44-45 36.587731691829006 40.0 35.0 41.0 30.0 41.0 46-47 36.415395345096435 40.0 35.0 41.0 30.0 41.0 48-49 36.230471624390646 39.5 35.0 41.0 30.0 41.0 50-51 35.98562163213322 39.0 35.0 41.0 29.5 41.0 52-53 35.71359642857571 39.0 35.0 41.0 29.0 41.0 54-55 35.44326798766471 38.5 35.0 40.5 29.0 41.0 56-57 35.17348212667321 38.0 35.0 40.0 28.5 41.0 58-59 34.7947499390798 37.0 35.0 40.0 28.0 41.0 60-61 34.48084164709017 37.0 34.0 39.0 28.0 41.0 62-63 34.03546636064519 36.0 34.0 39.0 26.5 41.0 64-65 33.53450544200457 35.5 34.0 38.5 26.0 40.0 66-67 33.174871587418394 35.0 33.5 37.0 26.0 40.0 68-69 32.94587225123851 35.0 34.0 37.0 26.0 39.0 70-71 32.763115849215595 35.0 34.0 36.0 26.0 39.0 72-73 32.50199866275657 35.0 34.0 36.0 26.0 38.0 74-75 32.169823913609754 35.0 33.0 35.0 25.5 37.0 76-77 31.949380414545466 35.0 33.0 35.0 25.0 37.0 78-79 31.756370812561443 35.0 33.0 35.0 24.5 36.0 80-81 31.579785656923836 35.0 33.0 35.0 24.0 36.0 82-83 31.472414852765176 35.0 33.0 35.0 24.0 36.0 84-85 31.21897901344086 35.0 33.0 35.0 23.0 35.0 86-87 31.009746631983166 35.0 33.0 35.0 20.5 35.0 88-89 30.706437854792647 35.0 32.0 35.0 18.0 35.0 90-91 30.511811316632595 35.0 32.0 35.0 15.5 35.0 92-93 30.382294368812698 35.0 32.0 35.0 11.0 35.0 94-95 30.142881579531775 35.0 31.5 35.0 7.0 35.0 96-97 29.972761767802204 35.0 31.0 35.0 6.0 35.0 98-99 29.75021817234595 35.0 31.0 35.0 2.0 35.0 100-101 29.56104564273513 35.0 31.0 35.0 2.0 35.0 102-103 28.809350380586203 34.0 29.5 35.0 2.0 35.0 104-105 29.24040791926603 35.0 30.0 35.0 2.0 35.0 106-107 29.14040635874856 35.0 30.0 35.0 2.0 35.0 108-109 28.89433916286641 35.0 30.0 35.0 2.0 35.0 110-111 28.700267208606377 35.0 29.0 35.0 2.0 35.0 112-113 28.494091640788085 35.0 29.0 35.0 2.0 35.0 114-115 28.079303096906933 34.5 29.0 35.0 2.0 35.0 116-117 27.834953670637184 34.0 28.5 35.0 2.0 35.0 118-119 27.56738374444966 34.0 28.0 35.0 2.0 35.0 120-121 27.177327001633742 34.0 27.0 35.0 2.0 35.0 122-123 26.53707789502999 34.0 24.5 35.0 2.0 35.0 124-125 23.5252227638685 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29445665616305305 1101 2 0.37478883680362785 1101 3 0.3845597569872723 1101 4 0.3880461371831885 1101 5 0.1954287351569377 1101 6 0.3674436447706597 1101 7 0.18287326959039518 1101 8 0.2961547457391802 1101 9 0.2835268626412031 1101 10-11 0.5059917106720135 1101 12-13 0.29178342548902236 1101 14-15 0.5146034466573326 1101 16-17 0.429851459611875 1101 18-19 0.42416944957504654 1101 20-21 0.3480550378135092 1101 22-23 0.33314963824169297 1101 24-25 0.3214475002920878 1101 26-27 0.3739173633115769 1101 28-29 0.17826256147072428 1101 30-31 0.15642515528339374 1101 32-33 0.2758013733803537 1101 34-35 0.011070074399896157 1101 36-37 0.12755753965133465 1101 38-39 0.1339150797766493 1101 40-41 -0.009051476033519634 1101 42-43 0.055301177186620976 1101 44-45 0.16115336115363732 1101 46-47 0.008432287828107121 1101 48-49 0.06041711930562599 1101 50-51 0.12250206534651653 1101 52-53 0.09130969463009819 1101 54-55 0.034428371799769764 1101 56-57 -0.1728382796017982 1101 58-59 -0.18732026706966565 1101 60-61 -0.1332587940033747 1101 62-63 -0.15346084836257035 1101 64-65 -0.16765971389717294 1101 66-67 -0.25532806550933884 1101 68-69 -0.16589087943245318 1101 70-71 -0.1980332297823182 1101 72-73 -0.11246263228917286 1101 74-75 -0.11948580567542422 1101 76-77 -0.06962192195952 1101 78-79 -0.13620607530437212 1101 80-81 -0.1152340432336807 1101 82-83 -0.16999923156640406 1101 84-85 -0.1846263194640123 1101 86-87 -0.2307412219863707 1101 88-89 -0.09421138165542331 1101 90-91 -0.1632014368713044 1101 92-93 -0.3403119751425798 1101 94-95 -0.14801771921813156 1101 96-97 -0.1949948933596488 1101 98-99 -0.34070032705888664 1101 100-101 -0.24369477718110133 1101 102-103 -0.11339383099809908 1101 104-105 -0.2674555281438522 1101 106-107 -0.16129341729171287 1101 108-109 -0.08235227813657886 1101 110-111 -0.42644794350139037 1101 112-113 -0.36065625570737225 1101 114-115 -0.3293605552228307 1101 116-117 -0.35239895904316043 1101 118-119 -0.37060403963837274 1101 120-121 -0.5206888440745949 1101 122-123 -0.3462088399170362 1101 124-125 -0.04606395806251484 1102 1 0.24016516726434745 1102 2 0.17784062085759444 1102 3 0.22826462810412806 1102 4 0.259954293517886 1102 5 0.3459234631142607 1102 6 0.42802267925160464 1102 7 0.41527012021255416 1102 8 0.1784182291779146 1102 9 0.2630758540621798 1102 10-11 0.381518832512576 1102 12-13 0.44101825394156435 1102 14-15 0.286863319785418 1102 16-17 0.3593799266640829 1102 18-19 0.42800025294334887 1102 20-21 0.26441287729809915 1102 22-23 0.4287425040040773 1102 24-25 0.49220983634442206 1102 26-27 0.4014171325154692 1102 28-29 0.3444213393063862 1102 30-31 0.2986858197124391 1102 32-33 0.4151383455990896 1102 34-35 0.4620427975962258 1102 36-37 0.23876458684584634 1102 38-39 0.16526543487295697 1102 40-41 0.2809665095779934 1102 42-43 0.17009133406203603 1102 44-45 0.2005617977407539 1102 46-47 0.2553985553273961 1102 48-49 0.3530501073240089 1102 50-51 0.1808584508155846 1102 52-53 0.24647351041523535 1102 54-55 0.3107329468381792 1102 56-57 0.2875332032302609 1102 58-59 0.18685669749983447 1102 60-61 0.25928006217107935 1102 62-63 0.3669481223527029 1102 64-65 0.3716691804575518 1102 66-67 0.3043286087845942 1102 68-69 0.4209799538733989 1102 70-71 0.42478657120549457 1102 72-73 0.293512679120731 1102 74-75 0.3774697462899752 1102 76-77 0.45263753903264003 1102 78-79 0.5257835355733746 1102 80-81 0.49474689960142015 1102 82-83 0.3903164480622152 1102 84-85 0.38753167366485997 1102 86-87 0.5785548333783197 1102 88-89 0.5148162907987306 1102 90-91 0.6748534629302085 1102 92-93 0.667452665573812 1102 94-95 0.5883727284603886 1102 96-97 0.5789963627646912 1102 98-99 0.513653311936423 1102 100-101 0.58742593578107 1102 102-103 0.5797066840069434 1102 104-105 0.5075961537004083 1102 106-107 0.34592761614734613 1102 108-109 0.32026165271690843 1102 110-111 0.5052112662527151 1102 112-113 0.4229872236429735 1102 114-115 0.5321465269208083 1102 116-117 0.7240666787124432 1102 118-119 0.6387407301919552 1102 120-121 0.5190155061024555 1102 122-123 0.3274623514525068 1102 124-125 0.43571839790422473 1103 1 0.33398670985590684 1103 2 0.4047070788681708 1103 3 0.3818275667680169 1103 4 0.3559319142112187 1103 5 0.3903723852320198 1103 6 0.2999955455967154 1103 7 0.3829320847995632 1103 8 0.15858309466999998 1103 9 0.1376587509530367 1103 10-11 0.38994674580530386 1103 12-13 0.26681772770170653 1103 14-15 0.27761531278561336 1103 16-17 0.3200244450511178 1103 18-19 0.33061490925665993 1103 20-21 0.3752039305932158 1103 22-23 0.39283988346755905 1103 24-25 0.40560958733700403 1103 26-27 0.2542248166210115 1103 28-29 0.26897703041173315 1103 30-31 0.3395262511162258 1103 32-33 0.26506080519027364 1103 34-35 0.16853980574384764 1103 36-37 0.2312477145546623 1103 38-39 0.4259501739169451 1103 40-41 0.4059441647685702 1103 42-43 0.30426172225017467 1103 44-45 0.3836402695753165 1103 46-47 0.2968693455653053 1103 48-49 0.2720599735374023 1103 50-51 0.2932775390846416 1103 52-53 0.35746409893830844 1103 54-55 0.3526514172928543 1103 56-57 0.2588573644766825 1103 58-59 0.20840140593534784 1103 60-61 0.1787219280332195 1103 62-63 -0.022522689837998655 1103 64-65 -0.0995487449405772 1103 66-67 0.15609471115988072 1103 68-69 0.14384551495548692 1103 70-71 0.0013579209260683456 1103 72-73 -0.09806089724050082 1103 74-75 -0.05877188812699785 1103 76-77 -0.05188865692428024 1103 78-79 0.07930092218719409 1103 80-81 0.13902066977447092 1103 82-83 0.08663206339906182 1103 84-85 0.1361866729435519 1103 86-87 0.14037116061295762 1103 88-89 0.07824144431149094 1103 90-91 -0.03203712747453835 1103 92-93 0.09083427414920209 1103 94-95 0.14126720450988728 1103 96-97 0.07888659878859983 1103 98-99 0.2862482264146067 1103 100-101 0.3409172142491421 1103 102-103 0.34761232462086866 1103 104-105 0.3519457394198007 1103 106-107 0.1693483477399269 1103 108-109 0.2017667363570581 1103 110-111 0.238486764424362 1103 112-113 0.2796495833802872 1103 114-115 0.23950518228073747 1103 116-117 0.1900998242971106 1103 118-119 0.40464474629903435 1103 120-121 0.4330193509777338 1103 122-123 0.23079752986600965 1103 124-125 0.08440271984510517 1104 1 -0.04373292866713996 1104 2 0.07288239190045331 1104 3 0.10521589727746772 1104 4 0.03666739987670553 1104 5 0.12556270198045638 1104 6 0.057114945747152035 1104 7 -0.03439793265218327 1104 8 0.002222479088445084 1104 9 0.023720682967500295 1104 10-11 0.17453544837940171 1104 12-13 0.17480575224480788 1104 14-15 0.23854665579242607 1104 16-17 0.248081229772545 1104 18-19 0.017160914122811732 1104 20-21 0.12650982066965355 1104 22-23 0.16002805875067594 1104 24-25 0.23276739030125526 1104 26-27 0.347329956287318 1104 28-29 0.22061909465270446 1104 30-31 0.25423775895006884 1104 32-33 0.27973029125355 1104 34-35 0.2893770708294312 1104 36-37 0.30227647030648797 1104 38-39 0.14053599029825392 1104 40-41 0.21436190304024194 1104 42-43 0.2160664750470147 1104 44-45 0.03680326768657949 1104 46-47 0.15706770028587158 1104 48-49 0.17769193759906443 1104 50-51 0.17136355959306115 1104 52-53 0.100689023082289 1104 54-55 0.1597980343907821 1104 56-57 0.17282788343806743 1104 58-59 0.07825429982963072 1104 60-61 0.07344056910000774 1104 62-63 0.08077945865587566 1104 64-65 0.05064992382168754 1104 66-67 0.1465287766091663 1104 68-69 0.20883457583620668 1104 70-71 0.20283548713278776 1104 72-73 0.024956340658050635 1104 74-75 0.07291364845811188 1104 76-77 0.046870728810151974 1104 78-79 0.1897325650290611 1104 80-81 0.18867066282068734 1104 82-83 0.360480539773409 1104 84-85 0.3786537845524762 1104 86-87 0.413883911453766 1104 88-89 0.4990218080315998 1104 90-91 0.4015408094509354 1104 92-93 0.42400522798357443 1104 94-95 0.38851186243622493 1104 96-97 0.2525004411486371 1104 98-99 0.2715308863777288 1104 100-101 0.18924516650283962 1104 102-103 0.24622321349347231 1104 104-105 0.1593316263857254 1104 106-107 0.21621757628900795 1104 108-109 0.19302964758345098 1104 110-111 0.21176891892988792 1104 112-113 0.23200347547855316 1104 114-115 0.24903896494294386 1104 116-117 0.24723896421166458 1104 118-119 0.28153815605915256 1104 120-121 0.3277895846098069 1104 122-123 0.15612626423309095 1104 124-125 -0.007280633402462655 1105 1 -0.15609991022590108 1105 2 -0.0732778873209341 1105 3 -0.12049517002751742 1105 4 -0.11279825071538596 1105 5 -0.2657469020861143 1105 6 -0.2030038428143257 1105 7 -0.22319522691272908 1105 8 -0.2117875166731693 1105 9 -0.32617364186346265 1105 10-11 -0.1128028743010745 1105 12-13 -0.21502183935692187 1105 14-15 -0.1538758798996156 1105 16-17 -0.14044782307642123 1105 18-19 -0.030703679433131015 1105 20-21 -0.1907453841524358 1105 22-23 -0.16053607313421026 1105 24-25 -0.09227406906856572 1105 26-27 0.047105805457704264 1105 28-29 -0.11112840000664193 1105 30-31 -0.201969626080178 1105 32-33 0.0012776431163032953 1105 34-35 0.019258964960840785 1105 36-37 -0.03647716005514212 1105 38-39 -0.02252164889286945 1105 40-41 0.014580364762494469 1105 42-43 -0.033012600700601524 1105 44-45 -0.19154541201383068 1105 46-47 -0.0243977568765672 1105 48-49 -0.17537193116460514 1105 50-51 -0.1955262623094356 1105 52-53 -0.05202724122226243 1105 54-55 -0.06539674642213811 1105 56-57 -0.13646043767790417 1105 58-59 -0.14087589254753397 1105 60-61 -0.11942443691622628 1105 62-63 -0.13251797641007101 1105 64-65 -0.09078697402184588 1105 66-67 -0.19598606484963454 1105 68-69 0.0034229546966244584 1105 70-71 -0.044467875983031036 1105 72-73 -0.05409878394762302 1105 74-75 -0.10650799688862378 1105 76-77 -0.029345531227569666 1105 78-79 -0.022578521049979372 1105 80-81 -0.17877100249481614 1105 82-83 -0.19421667171062396 1105 84-85 -0.18103064066278307 1105 86-87 -0.1560835752977212 1105 88-89 -0.1494724274803758 1105 90-91 -0.09773667391553786 1105 92-93 -0.04595272951310392 1105 94-95 -0.1150630303952127 1105 96-97 -0.06887936650264237 1105 98-99 0.06987549836638607 1105 100-101 -0.0390743565224021 1105 102-103 -0.06813623568715244 1105 104-105 -0.0247757100026611 1105 106-107 0.006317960588212657 1105 108-109 0.032915410307850834 1105 110-111 -0.20161655507231657 1105 112-113 -0.05256069623008486 1105 114-115 0.06312249241706525 1105 116-117 -0.08238293771381677 1105 118-119 0.06469996280586798 1105 120-121 0.10111848043940697 1105 122-123 0.02380900547257525 1105 124-125 0.5186453399326929 1106 1 0.2973120423568645 1106 2 0.4378577923056213 1106 3 0.35362804107745305 1106 4 -0.17003060745022935 1106 5 0.24949171962016692 1106 6 0.2631960220588354 1106 7 0.3757299005364487 1106 8 0.4537305718963296 1106 9 0.49395411441685866 1106 10-11 0.5904002459003834 1106 12-13 0.6893022193618208 1106 14-15 0.6806183157886068 1106 16-17 0.6431895456355718 1106 18-19 0.48880679289657536 1106 20-21 0.5970164240902989 1106 22-23 0.5991347087587968 1106 24-25 0.6074587483259464 1106 26-27 0.6942343602147858 1106 28-29 0.6426090854058017 1106 30-31 0.5901715200410607 1106 32-33 0.745854143331286 1106 34-35 0.8022606233356271 1106 36-37 0.8782578070844664 1106 38-39 0.9512514608669775 1106 40-41 0.8312777420734747 1106 42-43 0.7220730819970242 1106 44-45 0.8532737022911903 1106 46-47 0.9566660598423056 1106 48-49 0.7925385165965935 1106 50-51 0.7085551823192731 1106 52-53 0.7623944773453957 1106 54-55 0.7751136644862839 1106 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2216 90-91 -1.8012600316561809 2216 92-93 -1.6148700476841533 2216 94-95 -1.45195388068419 2216 96-97 -1.4368931183414553 2216 98-99 -1.3473740787755908 2216 100-101 -1.3747379543692695 2216 102-103 -1.552270657707723 2216 104-105 -1.8946191836642505 2216 106-107 -1.5052790929040185 2216 108-109 -1.6350790581031447 2216 110-111 -1.904502457784666 2216 112-113 -1.8614352051913734 2216 114-115 -1.8292247415129523 2216 116-117 -1.8827633721969939 2216 118-119 -2.1013576230191617 2216 120-121 -2.071626598154335 2216 122-123 -1.7141360973631912 2216 124-125 -1.469092603792756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13954.0 3 2605.0 4 1625.0 5 1402.0 6 1763.0 7 2141.0 8 3065.0 9 2866.0 10 3122.0 11 3157.0 12 2963.0 13 2734.0 14 2670.0 15 2805.0 16 2835.0 17 2878.0 18 2923.0 19 2944.0 20 3014.0 21 3534.0 22 4005.0 23 5404.0 24 7330.0 25 9775.0 26 11886.0 27 11299.0 28 12961.0 29 15912.0 30 18948.0 31 24019.0 32 32041.0 33 43226.0 34 62703.0 35 80530.0 36 166283.0 37 229191.0 38 32480.0 39 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.20362050247486 9.732820527001081 6.161851392764525 11.90170757775953 2 10.778346059645584 34.6772799308457 42.5531863804447 11.991187629064015 3 40.219261229874945 20.25861396265692 17.761312109909134 21.760812697559 4 13.896202595475387 16.13113525934944 49.68562067255685 20.287041472618323 5 16.390484810798224 32.8177031689287 28.051465380432305 22.740346639840773 6 29.307958519044913 40.532760663435994 14.446790555748287 15.712490261770803 7 44.699395958975146 23.074199093458304 16.182105404678676 16.044299542887874 8 42.80514828570537 23.036441815296488 16.00628530683485 18.152124592163293 9 58.593229514907144 10.123797051102146 9.652880895304882 21.630092538685826 10-11 41.168731551382436 25.457681767274025 17.92710462789461 15.446482053448923 12-13 28.73380813077617 29.94302910845236 23.304587801314916 18.018574959456558 14-15 25.063831166414786 30.35002406798094 21.793946872783014 22.79219789282126 16-17 22.37481949014293 35.392296085381915 21.552366764819215 20.68051765965594 18-19 22.72113432814321 26.929249739213525 25.747577896830588 24.602038035812676 20-21 23.92063208159826 28.553868462782255 22.134979959354524 25.390519496264965 22-23 25.657788122541437 24.221751932940965 22.833671645517654 27.286788298999948 24-25 22.7837951064573 23.06997000205268 33.52981848781056 20.61641640367946 26-27 18.333615429584572 23.199715743011513 31.771180326727478 26.69548850067643 28-29 21.432816003918386 24.360321057964264 23.374782111798854 30.832080826318492 30-31 21.61736831933469 29.203163769105835 21.082651007073945 28.09681690448553 32-33 28.977909074649155 26.479760688644355 19.019947014429984 25.522383222276506 34-35 28.005046473410584 19.58881565126996 25.12889274083286 27.277245134486595 36-37 24.59111441353953 25.88472337427091 28.134329343610343 21.38983286857922 38-39 22.605596015638785 30.16666326553885 24.053756225564396 23.173984493257965 40-41 20.970113689699506 30.698139503059213 25.140416562132 23.191330245109278 42-43 21.873773343240618 23.33585817056936 22.988162874809287 31.802205611380735 44-45 22.776652738047936 23.92801452961802 22.978019511269938 30.317313221064108 46-47 24.598856980974894 22.460227811542307 23.73475044324698 29.20616476423582 48-49 17.78599783688271 22.537713505798525 26.537019675724473 33.13926898159429 50-51 22.67503904294664 24.91588210650652 24.88731263286906 27.521766217677783 52-53 24.94337122189718 22.47421244884804 28.404719004822 24.17769732443278 54-55 20.224726519313805 24.24209867992226 29.58314977246455 25.950025028299383 56-57 19.664763427558974 26.547394615111752 26.659212163444483 27.128629793884784 58-59 22.269304501372655 23.902806170526148 31.501625939161425 22.326263388939775 60-61 23.747654722305917 29.21390733167118 28.064886316302484 18.973551629720415 62-63 24.791160748904336 25.89462665819986 26.352518495132987 22.96169409776282 64-65 29.76130240810332 19.83760713001693 28.879713675729242 21.52137678615051 66-67 29.909393846272035 23.994305304805437 24.74323454845773 21.353066300464796 68-69 22.340948501725975 28.396228850005734 25.76618365839004 23.496638989878253 70-71 23.579269006462305 28.624307020887674 22.680069515522433 25.116354457127592 72-73 22.712024934683303 28.07060505499927 23.00990389007228 26.207466120245144 74-75 20.833963596026823 28.202814933264186 26.769837668340163 24.193383802368828 76-77 21.796684716782046 27.45786272389085 24.27834550345657 26.46710705587053 78-79 21.718697388330465 29.104332935797935 21.422016338764983 27.75495333710662 80-81 20.265290198667547 22.36288338034932 27.02850969329572 30.343316727687412 82-83 22.87670591568164 18.56385577457485 28.95258067575208 29.606857633991428 84-85 24.149289265962025 19.735828691455765 31.565787996611867 24.549094045970346 86-87 26.68068361745368 21.89519338230263 25.561288247008846 25.862834753234843 88-89 22.76791878123833 25.16799513993692 26.824414613326038 25.239671465498713 90-91 19.34358593924902 27.77836797384091 25.9104274439969 26.967618642913177 92-93 22.232533681655234 23.33896795230423 26.34534531650311 28.083153049537422 94-95 20.517824374074443 21.996850938205515 28.373130807320756 29.11219388039928 96-97 20.858137868196536 20.66288281215299 28.742048443421652 29.736930876228822 98-99 20.638150372284393 21.97675360805251 30.92862523370937 26.45647078595373 100-101 19.21427285276994 25.66088470079827 28.784706800312104 26.340135646119684 102-103 19.883933512352698 25.64098254981352 27.718751538010007 26.75633239982378 104-105 19.80178944089425 23.626093642245067 26.89563155307552 29.67648536378516 106-107 18.93200503693069 24.86396325777537 24.80604335649729 31.397988348796645 108-109 21.201250334610958 25.458702105618226 25.854653403068458 27.485394156702363 110-111 21.530459500370323 26.389670814842205 24.540697695355778 27.539171989431694 112-113 19.002660715818383 26.543776367825807 25.789556765438665 28.66400615091715 114-115 20.32466499602347 23.31651685899071 27.83895799882955 28.519860146156272 116-117 23.07777258939064 23.189949787349484 26.396333024030767 27.335944599229105 118-119 21.00535170491744 24.379688748205588 26.981181939947984 27.633777606928987 120-121 19.529724155895913 24.73353716434806 27.064869400499138 28.671869279256896 122-123 20.824915157997598 26.015252953414027 27.853206229389727 25.30662565919865 124-125 20.8197707650191 28.220691314942393 24.3010739749105 26.658463945128002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 8.0 2 14.0 3 16.0 4 20.0 5 25.0 6 26.0 7 28.0 8 31.0 9 37.5 10 39.5 11 40.0 12 54.0 13 72.5 14 99.5 15 122.5 16 153.5 17 202.0 18 238.0 19 296.0 20 386.5 21 470.0 22 578.0 23 697.5 24 855.0 25 1029.0 26 1190.0 27 1432.5 28 1708.5 29 2114.0 30 2647.5 31 3252.0 32 4154.5 33 5230.5 34 6106.0 35 6822.5 36 8163.5 37 9449.5 38 9469.5 39 9530.5 40 9374.0 41 8866.0 42 8744.0 43 9430.5 44 10584.5 45 13070.0 46 22588.5 47 42637.5 48 62799.5 49 78939.5 50 85531.5 51 78852.5 52 65298.0 53 49377.0 54 38413.5 55 32828.5 56 27825.0 57 22449.0 58 18739.0 59 14988.0 60 11852.0 61 9521.0 62 7213.5 63 5233.0 64 3959.0 65 3353.5 66 2909.5 67 2525.5 68 1996.0 69 1333.5 70 919.5 71 738.5 72 647.0 73 570.5 74 495.0 75 435.5 76 306.0 77 188.0 78 160.0 79 146.5 80 128.5 81 104.5 82 65.5 83 34.0 84 16.5 85 14.0 86 13.5 87 5.5 88 4.5 89 5.0 90 3.5 91 1.5 92 1.0 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15533150792802894 2 0.016445453312318364 3 0.009363104805553522 4 0.017525811559113 5 0.0037212339611815276 6 0.0 7 1.2003980519940412E-4 8 3.6011941559821236E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.60318441595233E-4 28-29 0.007322428117163651 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.2013931819791445E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0018606169805907638 66-67 1.2003980519940412E-4 68-69 0.0058219305521711 70-71 0.017945950877310914 72-73 6.001990259970206E-5 74-75 0.008522826169157692 76-77 7.202388311964247E-4 78-79 9.002985389955309E-4 80-81 8.402786363958289E-4 82-83 0.0 84-85 0.018065990682510323 86-87 0.002160716493589274 88-89 0.017525811559113 90-91 6.001990259970206E-5 92-93 0.013324418377133858 94-95 0.013264398474534155 96-97 0.0052817514287737816 98-99 0.002940975227385401 100-101 8.402786363958289E-4 102-103 0.0 104-105 4.2013931819791445E-4 106-107 0.001200398051994041 108-109 0.0 110-111 0.0 112-113 0.0012604179545937432 114-115 0.005341771331373484 116-117 0.0 118-119 0.07346436078203532 120-121 0.0016805572727916578 122-123 0.004561512597577357 124-125 0.24392088416518917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 833057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.60239567237874 #Duplication Level Percentage of deduplicated Percentage of total 1 82.3044054444882 33.417560354369925 2 9.01828408193293 7.323278771611114 3 2.7060302988845693 3.2961393869026967 4 1.3688597313263637 2.223159377251963 5 0.8178858700348772 1.6604062855001913 6 0.5606742340876562 1.3658830257440948 7 0.41490747171330006 1.179236613375079 8 0.3249408511228212 1.0554701605926637 9 0.2543748847508456 0.9295406747792615 >10 1.853390554752516 14.958715919179994 >50 0.2112882910761411 5.917941417953005 >100 0.14282613656824195 11.12968695456487 >500 0.012098908263010931 3.2594682302935203 >1k 0.008262669057666 6.505037831168056 >5k 0.0014754766174403572 4.476556830621472 >10k+ 2.9509532348807145E-4 1.3019181660920964 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 10866 1.3043525232967252 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8666 1.040264951858036 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8212 0.9857668802975067 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 7851 0.9424325106205217 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6591 0.7911823560692725 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6042 0.7252805030147997 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4575 0.5491821087872739 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 3806 0.4568714985889321 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3445 0.4135371289119472 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3166 0.3800460232613134 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2623 0.31486440903803703 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2565 0.30790210033647153 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2380 0.2856947363745818 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2251 0.2702096015038587 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1983 0.23803893371041837 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1971 0.23659845604802554 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1970 0.23647841624282614 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1880 0.22567483377487976 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1806 0.21679188819012382 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1754 0.21054981831975483 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1746 0.2095894998781596 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1392 0.16709540883757054 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1389 0.16673528942197233 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1388 0.16661524961677293 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1352 0.16229381662959438 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1351 0.16217377682439496 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1339 0.16073329916200213 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1265 0.15185035357724622 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1227 0.14728884097966888 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1201 0.14416780604448434 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1196 0.1435676070184873 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1146 0.13756561675851714 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1097 0.13168366630374634 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1028 0.12340091974498744 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 975 0.11703881006941902 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 902 0.10827590428986252 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 901 0.10815586448466312 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 886 0.10635526740667206 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 884 0.10611518779627324 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 842 0.10107351597789828 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.2003980519940412E-4 0.0 0.0 0.0 0.0 3 1.2003980519940412E-4 0.0 0.0 0.0 0.0 4 1.2003980519940412E-4 0.0 0.0 0.0 0.0 5 1.2003980519940412E-4 0.0 0.0 0.0 0.0 6 1.2003980519940412E-4 0.0 0.0 0.0 0.0 7 1.2003980519940412E-4 0.0 0.0 0.0 0.0 8 1.2003980519940412E-4 0.0 0.0 0.0 0.0 9 1.2003980519940412E-4 0.0 0.0 1.2003980519940412E-4 0.0 10-11 1.2003980519940412E-4 0.0 0.0 1.2003980519940412E-4 0.0 12-13 1.2003980519940412E-4 0.0 0.0 2.4007961039880824E-4 0.0 14-15 2.4007961039880824E-4 0.0 0.0 2.4007961039880824E-4 0.0 16-17 2.4007961039880824E-4 0.0 0.0 2.4007961039880824E-4 0.0 18-19 2.4007961039880824E-4 0.0 0.0 3.000995129985103E-4 0.0 20-21 2.4007961039880824E-4 0.0 0.0 0.001200398051994041 0.0 22-23 2.4007961039880824E-4 0.0 0.0 0.0034211344481830178 0.0 24-25 2.4007961039880824E-4 0.0 0.0 0.007082348506764843 0.0 26-27 3.6011941559821236E-4 0.0 0.0 0.011463801396543093 0.0 28-29 3.6011941559821236E-4 0.0 0.0 0.02304764259828559 0.0 30-31 3.6011941559821236E-4 0.0 0.0 0.06782248993766332 6.001990259970206E-5 32-33 3.6011941559821236E-4 0.0 0.0 0.1519703933824456 1.2003980519940412E-4 34-35 3.6011941559821236E-4 0.0 0.0 0.25268378994474566 1.2003980519940412E-4 36-37 3.6011941559821236E-4 0.0 0.0 0.379325784430117 1.2003980519940412E-4 38-39 3.6011941559821236E-4 0.0 0.0 0.5767312440805371 1.2003980519940412E-4 40-41 3.6011941559821236E-4 0.0 0.0 0.8833129065598152 1.2003980519940412E-4 42-43 3.6011941559821236E-4 0.0 0.0 1.5417312380785468 1.2003980519940412E-4 44-45 3.6011941559821236E-4 0.0 0.0 2.4772014399975033 1.2003980519940412E-4 46-47 3.6011941559821236E-4 0.0 0.0 3.43265826948216 1.2003980519940412E-4 48-49 3.6011941559821236E-4 0.0 0.0 3.781854062807227 1.2003980519940412E-4 50-51 3.6011941559821236E-4 0.0 0.0 4.243587173506735 1.2003980519940412E-4 52-53 3.6011941559821236E-4 0.0 0.0 4.610008678877916 1.2003980519940412E-4 54-55 3.6011941559821236E-4 0.0 0.0 4.9662268008071475 1.2003980519940412E-4 56-57 3.6011941559821236E-4 0.0 0.0 5.526452571672767 1.2003980519940412E-4 58-59 3.6011941559821236E-4 0.0 0.0 6.183670505139505 1.2003980519940412E-4 60-61 3.6011941559821236E-4 0.0 0.0 7.153772190858488 1.2003980519940412E-4 62-63 3.6011941559821236E-4 0.0 0.0 8.3601122132099 1.2003980519940412E-4 64-65 3.6011941559821236E-4 0.0 0.0 9.428106360068998 1.2003980519940412E-4 66-67 3.6011941559821236E-4 0.0 0.0 10.265323981432243 1.2003980519940412E-4 68-69 3.6011941559821236E-4 0.0 0.0 10.909337536327048 1.2003980519940412E-4 70-71 3.6011941559821236E-4 0.0 0.0 11.658926099894725 1.2003980519940412E-4 72-73 3.6011941559821236E-4 0.0 0.0 12.400351956708844 1.2003980519940412E-4 74-75 3.6011941559821236E-4 0.0 0.0 13.189673695797527 1.2003980519940412E-4 76-77 3.6011941559821236E-4 0.0 0.0 14.110439021579555 1.2003980519940412E-4 78-79 3.6011941559821236E-4 0.0 0.0 15.352130766562192 1.2003980519940412E-4 80-81 3.6011941559821236E-4 0.0 0.0 17.801542991656035 1.2003980519940412E-4 82-83 3.6011941559821236E-4 0.0 0.0 19.512290275455342 1.2003980519940412E-4 84-85 3.6011941559821236E-4 0.0 0.0 20.299031158732234 1.2003980519940412E-4 86-87 3.6011941559821236E-4 6.001990259970206E-5 0.0 20.980257053238855 1.2003980519940412E-4 88-89 3.6011941559821236E-4 1.2003980519940412E-4 0.0 21.76279654333377 1.2003980519940412E-4 90-91 3.6011941559821236E-4 1.2003980519940412E-4 0.0 22.758406687657626 1.2003980519940412E-4 92-93 3.6011941559821236E-4 1.2003980519940412E-4 0.0 24.27895090011848 1.2003980519940412E-4 94-95 3.6011941559821236E-4 1.2003980519940412E-4 0.0 26.521474520951145 1.2003980519940412E-4 96-97 3.6011941559821236E-4 1.2003980519940412E-4 0.0 29.064697853808322 1.2003980519940412E-4 98-99 3.6011941559821236E-4 1.2003980519940412E-4 0.0 31.253863781229857 1.2003980519940412E-4 100-101 3.6011941559821236E-4 1.2003980519940412E-4 0.0 32.87506137035041 1.2003980519940412E-4 102-103 3.6011941559821236E-4 1.2003980519940412E-4 0.0 34.16338857965302 1.2003980519940412E-4 104-105 3.6011941559821236E-4 1.2003980519940412E-4 0.0 35.60440642116926 1.2003980519940412E-4 106-107 3.6011941559821236E-4 1.2003980519940412E-4 0.0 37.079335507654335 1.2003980519940412E-4 108-109 3.6011941559821236E-4 1.2003980519940412E-4 0.0 38.251404165621324 1.2003980519940412E-4 110-111 3.6011941559821236E-4 1.2003980519940412E-4 0.0 39.35228921910505 1.2003980519940412E-4 112-113 3.6011941559821236E-4 1.2003980519940412E-4 0.0 42.29656554113345 1.2003980519940412E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 1880 0.0 94.42501 1 AGTAGTA 1590 0.0 92.05446 2 GAGTTAG 1025 0.0 85.4323 1 GAGTCAG 4355 0.0 83.986374 1 GAGTAAG 690 0.0 81.15352 1 GAGTCCG 545 0.0 80.884224 1 GAGTCGG 585 0.0 79.42685 1 AGTTTCG 90 0.0 79.3315 2 GTTAACG 60 4.3655746E-11 79.32673 3 GAGTCGA 320 0.0 78.18581 1 AGTCCGT 230 0.0 77.606895 2 GAGTAAA 455 0.0 74.62633 1 AGTTAGT 815 0.0 74.46453 2 GAGTTAC 665 0.0 72.55912 1 GAGTCGC 345 0.0 72.520164 1 GAGTCAA 510 0.0 72.4186 1 GAGTCGT 585 0.0 72.298805 1 AGTCGAC 125 0.0 71.398346 2 TCGACGG 125 0.0 71.39405 4 GAGTGGT 710 0.0 69.638336 1 >>END_MODULE