##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833057 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53335366007368 33.0 31.0 34.0 31.0 34.0 2 32.569353597652984 34.0 31.0 34.0 31.0 34.0 3 32.77417271567252 34.0 31.0 34.0 31.0 34.0 4 36.0123424927706 37.0 35.0 37.0 35.0 37.0 5 36.06133793966079 37.0 35.0 37.0 35.0 37.0 6 36.182857835658304 37.0 36.0 37.0 35.0 37.0 7 36.21672946749142 37.0 37.0 37.0 35.0 37.0 8 36.21658061813297 37.0 37.0 37.0 35.0 37.0 9 38.04990654901165 39.0 39.0 39.0 35.0 39.0 10-11 37.73670529147465 39.0 38.0 39.0 35.0 39.0 12-13 37.63859315749102 39.0 37.0 39.0 35.0 39.0 14-15 39.166618250611904 41.0 39.0 41.0 35.0 41.0 16-17 39.254878117583786 41.0 39.0 41.0 35.5 41.0 18-19 39.26915505181518 41.0 39.0 41.0 35.0 41.0 20-21 39.278709019911005 41.0 39.0 41.0 35.0 41.0 22-23 39.14170578963984 41.0 39.0 41.0 35.0 41.0 24-25 38.938876931590514 41.0 38.0 41.0 35.0 41.0 26-27 38.83450052037256 40.0 38.0 41.0 35.0 41.0 28-29 38.706748757888114 40.0 38.0 41.0 35.0 41.0 30-31 38.59346539312436 40.0 38.0 41.0 35.0 41.0 32-33 38.45342815677679 40.0 38.0 41.0 34.5 41.0 34-35 38.07963320637123 40.0 37.0 41.0 34.0 41.0 36-37 37.920710707670665 40.0 37.5 41.0 33.5 41.0 38-39 37.60101169547822 40.0 37.0 41.0 33.0 41.0 40-41 37.34454785206775 40.0 37.0 41.0 33.0 41.0 42-43 37.15359573234485 40.0 37.0 41.0 33.0 41.0 44-45 36.971576974924886 40.0 36.0 41.0 32.0 41.0 46-47 36.788344014875335 40.0 35.5 41.0 32.0 41.0 48-49 36.57755411694518 40.0 35.0 41.0 31.0 41.0 50-51 36.31982685458498 39.5 35.0 41.0 31.0 41.0 52-53 36.048893413055765 39.0 35.0 41.0 30.5 41.0 54-55 35.78997955722117 39.0 35.0 41.0 30.0 41.0 56-57 35.54182666972368 38.5 35.0 41.0 30.0 41.0 58-59 35.22023583020129 37.0 35.0 40.0 30.0 41.0 60-61 34.894637461782324 37.0 35.0 39.5 29.0 41.0 62-63 34.453330924534576 36.0 35.0 39.0 28.5 41.0 64-65 33.97917609479303 36.0 34.5 38.5 28.0 41.0 66-67 33.538448749605365 35.0 34.0 37.0 27.0 40.0 68-69 33.19795044036603 35.0 34.0 37.0 27.0 39.0 70-71 33.0102021830439 35.0 34.0 36.0 27.0 39.0 72-73 32.73435671268592 35.0 34.0 36.0 26.0 38.5 74-75 32.4270049948563 35.0 34.0 35.0 26.0 37.0 76-77 32.27547634795698 35.0 34.0 35.0 26.0 37.0 78-79 32.29794359809713 35.0 34.0 35.0 27.0 36.0 80-81 32.228086433461336 35.0 34.0 35.0 27.0 36.0 82-83 32.11174385426207 35.0 34.0 35.0 27.0 36.0 84-85 31.936740223057967 35.0 33.5 35.0 26.0 35.0 86-87 31.825498735380652 35.0 33.0 35.0 25.5 35.0 88-89 31.661695418200676 35.0 33.0 35.0 25.0 35.0 90-91 31.62442605968139 35.0 33.0 35.0 24.5 35.0 92-93 31.664393312822533 35.0 33.0 35.0 25.0 35.5 94-95 31.594428712561083 35.0 33.0 35.0 25.0 35.0 96-97 31.57279033727584 35.0 33.0 35.0 25.0 35.0 98-99 31.465071417682104 35.0 33.0 35.0 24.0 35.0 100-101 31.41018201635662 35.0 33.0 35.0 24.0 35.0 102-103 30.706470265540055 34.0 31.5 35.0 22.0 35.0 104-105 31.298780875738395 35.0 33.0 35.0 24.0 35.0 106-107 31.37814939433916 35.0 33.0 35.0 24.0 35.0 108-109 31.303267963656747 35.0 33.0 35.0 24.0 35.0 110-111 31.21997114243083 35.0 33.0 35.0 23.5 35.0 112-113 31.157201728093035 35.0 33.0 35.0 23.0 35.0 114-115 30.999037280762302 35.0 33.0 35.0 19.5 35.0 116-117 30.789534209543884 35.0 33.0 35.0 18.0 35.0 118-119 30.509085812855545 35.0 33.0 35.0 6.0 35.0 120-121 30.143640231100633 35.0 33.0 35.0 2.0 35.0 122-123 29.692277959371328 35.0 32.5 35.0 2.0 35.0 124-125 27.231092830382554 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12207736521066437 1101 2 0.18068393307178354 1101 3 0.1654662011566188 1101 4 0.17468440342561564 1101 5 0.20288330471000648 1101 6 0.21913276574315432 1101 7 0.12942352855662875 1101 8 0.14183764442493896 1101 9 0.18392194691988806 1101 10-11 0.21266714682214882 1101 12-13 0.15763433022054585 1101 14-15 0.1891235180279054 1101 16-17 0.1864567785544864 1101 18-19 0.147487251704419 1101 20-21 0.11919457624230745 1101 22-23 0.0995611367161473 1101 24-25 0.1983057168590534 1101 26-27 0.11312163598997671 1101 28-29 0.1523433217990089 1101 30-31 0.26359654377016994 1101 32-33 0.2648729371237195 1101 34-35 0.3715215147213229 1101 36-37 0.404241360479098 1101 38-39 0.46500393785163396 1101 40-41 0.45983894950990845 1101 42-43 0.33863785559406523 1101 44-45 0.34502990860362814 1101 46-47 0.34389848539039747 1101 48-49 0.31125110995687777 1101 50-51 0.35842349389444905 1101 52-53 0.3809039698597374 1101 54-55 0.3649207393581335 1101 56-57 0.25111989636774723 1101 58-59 0.37465680029973214 1101 60-61 0.30677385123367173 1101 62-63 0.30272776971183646 1101 64-65 0.13627692055523255 1101 66-67 0.08272026206829963 1101 68-69 0.2626230573173558 1101 70-71 0.08207468944000595 1101 72-73 0.056103061923586495 1101 74-75 0.11076582792885858 1101 76-77 0.1715852289050659 1101 78-79 0.053161572223956455 1101 80-81 0.05866555589098965 1101 82-83 0.06459027936026018 1101 84-85 0.00576223740548798 1101 86-87 -0.1085154366535761 1101 88-89 -0.12784956549830184 1101 90-91 -0.14801241819921884 1101 92-93 -0.12123688681711542 1101 94-95 0.024012030204445978 1101 96-97 -0.013715824493200302 1101 98-99 -0.023914328995790157 1101 100-101 -0.0017809274608460157 1101 102-103 -0.06478428408260228 1101 104-105 0.04937101908019059 1101 106-107 0.11079952124840275 1101 108-109 0.08848972081026218 1101 110-111 0.03288246652099147 1101 112-113 0.1202963662544434 1101 114-115 0.07494567831239252 1101 116-117 -0.10814143891283479 1101 118-119 -0.307376225402308 1101 120-121 -0.11678890375801032 1101 122-123 -0.281200091761832 1101 124-125 -0.31385216506395963 1102 1 0.01690192719260608 1102 2 0.0926104871539053 1102 3 0.12771455096439865 1102 4 0.2367314660504931 1102 5 0.18874951549939567 1102 6 0.22569726666304035 1102 7 0.18563969219766818 1102 8 0.15557990451237913 1102 9 0.13341963566165305 1102 10-11 0.08209053570041647 1102 12-13 0.2840904064228269 1102 14-15 0.2518938791908525 1102 16-17 0.2746390283550184 1102 18-19 0.1907924334069122 1102 20-21 0.18814656405626806 1102 22-23 0.21294384942774514 1102 24-25 0.13907148188224028 1102 26-27 0.05298276267066626 1102 28-29 0.0808871961404165 1102 30-31 0.10856790856650633 1102 32-33 0.20171145897352005 1102 34-35 0.03435229501000947 1102 36-37 -0.0152441784957702 1102 38-39 0.04282467850933358 1102 40-41 0.21841096449187347 1102 42-43 0.05282241994991921 1102 44-45 0.1195124307435691 1102 46-47 0.1444894058712478 1102 48-49 0.12041528182744088 1102 50-51 0.1983915286325697 1102 52-53 0.27923457210571456 1102 54-55 0.20511421244400196 1102 56-57 0.2849504854253695 1102 58-59 0.3457050762527558 1102 60-61 0.31584949630881454 1102 62-63 0.3641248939922477 1102 64-65 0.2785338757610063 1102 66-67 0.3753215156209109 1102 68-69 0.40967315326260234 1102 70-71 0.335566304946866 1102 72-73 0.2706626699658443 1102 74-75 0.3960788698875888 1102 76-77 0.2627764830511552 1102 78-79 0.22198645732046884 1102 80-81 0.29132328854995393 1102 82-83 0.3388156286866675 1102 84-85 0.4751611454101585 1102 86-87 0.39378922740619515 1102 88-89 0.33284091053517173 1102 90-91 0.39587099019780325 1102 92-93 0.3460679585824593 1102 94-95 0.28328797333627875 1102 96-97 0.25943055299692475 1102 98-99 0.10090474333571109 1102 100-101 0.22233982176928535 1102 102-103 0.20244588228842275 1102 104-105 0.1809363442719203 1102 106-107 0.3685987813820013 1102 108-109 0.20729985144086172 1102 110-111 0.047620859124965165 1102 112-113 0.11390125319731936 1102 114-115 0.09232421361430454 1102 116-117 0.2567554611656355 1102 118-119 0.29558769127370255 1102 120-121 0.35963898234740554 1102 122-123 0.21845577474569566 1102 124-125 0.14769679008906067 1103 1 0.02424513719304855 1103 2 0.0023923390917062193 1103 3 0.13296440513511953 1103 4 0.12527163652962514 1103 5 0.15297508360637124 1103 6 0.18495601485193447 1103 7 0.11724654272422441 1103 8 0.12302077309040271 1103 9 0.18849371066495024 1103 10-11 0.28557922687475923 1103 12-13 0.1702624191178188 1103 14-15 0.18909594839860944 1103 16-17 0.3179503252634319 1103 18-19 0.2864709472653928 1103 20-21 0.24776094752769495 1103 22-23 0.24912597608909692 1103 24-25 0.2855048582848667 1103 26-27 0.34474168334580924 1103 28-29 0.37185111201730336 1103 30-31 0.3023542482828745 1103 32-33 0.28365105115330635 1103 34-35 0.2455530815472997 1103 36-37 0.2725228615774853 1103 38-39 0.21327284482372022 1103 40-41 0.250526727853277 1103 42-43 0.3599647618741102 1103 44-45 0.3148923081842483 1103 46-47 0.2442346750438773 1103 48-49 0.34011611227033 1103 50-51 0.269453902796009 1103 52-53 0.3445915715976682 1103 54-55 0.19530758520873803 1103 56-57 0.14895683919152702 1103 58-59 0.12761973632940737 1103 60-61 0.0811815636976263 1103 62-63 0.2562845315829563 1103 64-65 0.3049830738567607 1103 66-67 0.2766989751180091 1103 68-69 0.20367439516358843 1103 70-71 0.17087486716422262 1103 72-73 0.12184623923945992 1103 74-75 0.1083377027515553 1103 76-77 0.04552809344549047 1103 78-79 0.09569751462980491 1103 80-81 0.2293203625559599 1103 82-83 0.24128563176005713 1103 84-85 0.34164502892234694 1103 86-87 0.32638787385956647 1103 88-89 0.33039003775475706 1103 90-91 0.32739786982748953 1103 92-93 0.2627049628413012 1103 94-95 0.3394781127678925 1103 96-97 0.41985295613806173 1103 98-99 0.36547551914749477 1103 100-101 0.28092124782286376 1103 102-103 0.25104819485859764 1103 104-105 0.19145330123816962 1103 106-107 0.1357580269585128 1103 108-109 0.2156140806154987 1103 110-111 0.27790215283454955 1103 112-113 0.22901739916459007 1103 114-115 0.28915650366717927 1103 116-117 0.34975534199331904 1103 118-119 0.09671183119454696 1103 120-121 0.21459804038432395 1103 122-123 0.0545856067127275 1103 124-125 -0.061472186847961297 1104 1 0.007292119327068747 1104 2 0.070117675228488 1104 3 0.11370243304295968 1104 4 0.14098045108201518 1104 5 0.1064707416059889 1104 6 0.11100891442887217 1104 7 0.16223525965450847 1104 8 0.12162627839097695 1104 9 0.04376460154144013 1104 10-11 -0.0014384387186794356 1104 12-13 -0.005992364410239759 1104 14-15 0.20584932546848478 1104 16-17 0.20642327505714064 1104 18-19 0.26404986519732176 1104 20-21 0.18993278516514067 1104 22-23 0.24248436795630823 1104 24-25 0.2806125372026429 1104 26-27 0.28013208952135926 1104 28-29 0.22101763038649835 1104 30-31 0.1596641670657135 1104 32-33 0.09617787554866197 1104 34-35 -0.001953651656329214 1104 36-37 0.05828473422310054 1104 38-39 0.07005375288277094 1104 40-41 0.06628369546660906 1104 42-43 0.07587516129029126 1104 44-45 0.09486011143263084 1104 46-47 0.09278694279882416 1104 48-49 0.01123444467898338 1104 50-51 0.16491839290443977 1104 52-53 0.18078031664216354 1104 54-55 0.1651386457275663 1104 56-57 0.306007224939421 1104 58-59 0.2169922660549446 1104 60-61 0.051420130710383205 1104 62-63 0.01589121678306782 1104 64-65 0.10022489344429175 1104 66-67 0.07595903305121965 1104 68-69 0.25949792466331445 1104 70-71 0.2008037890590657 1104 72-73 0.3066246379557711 1104 74-75 0.277661511150221 1104 76-77 0.20480219352908335 1104 78-79 0.08125907353281292 1104 80-81 0.1852135794047527 1104 82-83 0.31458595582118676 1104 84-85 0.2919408262966634 1104 86-87 0.21812872664727934 1104 88-89 -0.009533560385516893 1104 90-91 8.431017024257415E-4 1104 92-93 0.12803715166710106 1104 94-95 0.10583893771057973 1104 96-97 0.012193179945111154 1104 98-99 0.15906413349082626 1104 100-101 -0.017762693131317064 1104 102-103 -0.06839876275026313 1104 104-105 0.01506766700104123 1104 106-107 -0.011986630220107486 1104 108-109 -0.0051856490095509855 1104 110-111 0.05300343128336138 1104 112-113 0.12516231296014269 1104 114-115 0.0798615422419715 1104 116-117 0.09115209480212272 1104 118-119 -0.06645526441320726 1104 120-121 0.010103430685816761 1104 122-123 -0.061164182804560596 1104 124-125 -0.10543989438840029 1105 1 -0.023379280676913083 1105 2 -0.03181832800292739 1105 3 0.040745320378718475 1105 4 0.0835361118860618 1105 5 0.01854985433280376 1105 6 0.017105930776523337 1105 7 0.06142712195535438 1105 8 0.04957515607232921 1105 9 0.08752948211285627 1105 10-11 0.07284494709666944 1105 12-13 0.009257287843063011 1105 14-15 -0.016235647038904233 1105 16-17 -0.06827171767502449 1105 18-19 0.009066918296610993 1105 20-21 0.05258635550669055 1105 22-23 0.08739503474814114 1105 24-25 0.09594553487864488 1105 26-27 0.08155095289460945 1105 28-29 0.09588713648664537 1105 30-31 0.07021575446889017 1105 32-33 0.10096668166103484 1105 34-35 0.1469025533108308 1105 36-37 -0.06686780496773537 1105 38-39 -0.09946671225864634 1105 40-41 -0.26209940333327353 1105 42-43 -0.2696136610360398 1105 44-45 -0.31218698584256543 1105 46-47 -0.1934320480104219 1105 48-49 -0.2795088087508404 1105 50-51 -0.3925394579214725 1105 52-53 -0.3297879114000182 1105 54-55 -0.17450746334968414 1105 56-57 -0.34440423974970713 1105 58-59 -0.26673333123021337 1105 60-61 -0.16141719779858477 1105 62-63 -0.21249515325353485 1105 64-65 -0.0854391249429085 1105 66-67 -0.1257276450505458 1105 68-69 -0.17075504149502763 1105 70-71 -0.187904470671441 1105 72-73 -0.2147102257474387 1105 74-75 -0.14358417579526872 1105 76-77 -0.15929854349477068 1105 78-79 -0.07414529399490277 1105 80-81 -0.032889729095636255 1105 82-83 -0.0429893750715209 1105 84-85 -0.052627071777287426 1105 86-87 -0.009872917504743839 1105 88-89 -0.09294085211441327 1105 90-91 -0.13214689400230029 1105 92-93 -0.15185981859877984 1105 94-95 -0.15070140232736762 1105 96-97 -0.11789892670289603 1105 98-99 -0.14507981230335787 1105 100-101 -0.22840331506453282 1105 102-103 -0.1323869976762566 1105 104-105 -0.04031650419557664 1105 106-107 -0.08222861640126666 1105 108-109 -0.03186329565340529 1105 110-111 -0.19077126299822922 1105 112-113 -0.14584542707040882 1105 114-115 -0.07672189278415686 1105 116-117 -0.08135017372943665 1105 118-119 0.0610603877433924 1105 120-121 0.07773838342355788 1105 122-123 0.09615883617714616 1105 124-125 0.24856907733389377 1106 1 0.02874282484485491 1106 2 0.11475129302772302 1106 3 0.17124713230673194 1106 4 0.14617354201934774 1106 5 0.2039070191261203 1106 6 0.19413436883315427 1106 7 0.18768472283982618 1106 8 0.16770057834368401 1106 9 0.20000414583130066 1106 10-11 0.24041757777045802 1106 12-13 0.1924677785991591 1106 14-15 0.2566437829715298 1106 16-17 0.24468817422350497 1106 18-19 0.24565557992672638 1106 20-21 0.18247702561294687 1106 22-23 0.29339282358175467 1106 24-25 0.3239106475867146 1106 26-27 0.3754211082904604 1106 28-29 0.42724270432645994 1106 30-31 0.3540496713044661 1106 32-33 0.4752311617298375 1106 34-35 0.42242924086073685 1106 36-37 0.46338217967694817 1106 38-39 0.37233477413654015 1106 40-41 0.3428480814649575 1106 42-43 0.29462379366575675 1106 44-45 0.2228016512190365 1106 46-47 0.34908683903582727 1106 48-49 0.39439942784396465 1106 50-51 0.32973497239606786 1106 52-53 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-0.8271829518779512 2216 50-51 -0.9109989324205188 2216 52-53 -0.8280926226169072 2216 54-55 -1.0601167921211143 2216 56-57 -0.8245794985608654 2216 58-59 -0.6015952894347549 2216 60-61 -0.5207332972569958 2216 62-63 -0.5915525242060866 2216 64-65 -0.5776117061123642 2216 66-67 -0.7297697269996064 2216 68-69 -0.7528283405589491 2216 70-71 -0.7413416039419687 2216 72-73 -0.5511148581777547 2216 74-75 -0.677796282482209 2216 76-77 -0.7700526357136326 2216 78-79 -0.7655198167300625 2216 80-81 -0.7598702748717336 2216 82-83 -0.7636939915241427 2216 84-85 -0.9189573803672282 2216 86-87 -1.143953070449875 2216 88-89 -0.970401279765742 2216 90-91 -0.8025760329146046 2216 92-93 -0.9728996912179682 2216 94-95 -0.9925566333703806 2216 96-97 -0.9693446328344741 2216 98-99 -1.2038160315394038 2216 100-101 -1.081783424484211 2216 102-103 -1.1679098265000256 2216 104-105 -1.1285607756401248 2216 106-107 -1.3755466331833546 2216 108-109 -1.2504635391755485 2216 110-111 -1.3103833355259589 2216 112-113 -0.935837328030722 2216 114-115 -1.0595260337625447 2216 116-117 -1.2234125638827642 2216 118-119 -0.8786689831210026 2216 120-121 -1.1859186911151554 2216 122-123 -1.2006409412369976 2216 124-125 -1.5971026195526612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 37.0 5 121.0 6 256.0 7 559.0 8 791.0 9 1395.0 10 2975.0 11 9140.0 12 13196.0 13 3925.0 14 3394.0 15 4466.0 16 2925.0 17 1873.0 18 1705.0 19 1725.0 20 1752.0 21 1951.0 22 2137.0 23 2352.0 24 2850.0 25 3902.0 26 4875.0 27 6369.0 28 8363.0 29 10748.0 30 13882.0 31 18359.0 32 24314.0 33 33058.0 34 56115.0 35 84294.0 36 172623.0 37 289634.0 38 46837.0 39 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 71.92251017941022 9.778488816181202 6.281944101956492 12.017056902452081 2 11.024295031156555 32.14449055339949 44.97467742071014 11.856536994733816 3 38.70239371375548 20.460424676822832 18.77902712539478 22.058154484026904 4 14.811952725585822 16.601087878780596 48.07962715033927 20.50733224529431 5 16.655919672522877 32.354216123853405 28.97413636794826 22.01572783567546 6 29.47362861220849 39.64717307509936 15.260436736158878 15.618761576533263 7 45.227877564200284 22.204483006564978 17.17613560656714 15.391503822667596 8 44.284124615722575 21.61148636887992 16.620831467714694 17.483557547682814 9 55.81178718863175 8.9648127319019 13.465705227853556 21.757694851612793 10-11 37.246310876686714 25.142217159209995 22.092365828508733 15.519106135594562 12-13 28.225019416438492 28.153355652734447 26.052899141355272 17.568725789471788 14-15 24.56062430301888 27.997063826364823 25.382416809414 22.059895061202294 16-17 21.497088434524887 33.68569017486198 25.109866431708756 19.70735495890437 18-19 22.243075803936584 25.71318649264096 28.503631804306305 23.54010589911615 20-21 22.96961672490598 26.54338178540004 26.081108495577134 24.405892994116847 22-23 24.377083440868994 23.400259526058843 26.503648609879036 25.71900842319313 24-25 20.872821427585386 21.644437295407158 37.65594671192968 19.82679456507778 26-27 17.217309259750532 22.43519951215823 34.78975628318351 25.557734944907732 28-29 20.764245423782526 23.002087492212418 26.280614651818546 29.95305243218651 30-31 20.704285541085422 27.76808789794696 25.024278050601577 26.50334851036604 32-33 28.479863922876824 24.12169875530726 22.746882866358483 24.65155445545743 34-35 26.60286150887634 18.199775045405055 29.241336427159247 25.956027018559354 36-37 23.75893846399466 25.015335085114227 30.709843384066154 20.51588306682496 38-39 22.429797720924256 29.225611212678125 25.729571926050674 22.61501914034694 40-41 20.55237516760558 28.411441233913166 27.517084665275004 23.51909893320625 42-43 21.854687014213916 22.21594680796152 24.429300756130733 31.500065421693833 44-45 22.593532015216244 22.925141977079598 24.58343186600677 29.897894141697385 46-47 23.957664361502275 21.67834854037599 24.799563535268295 29.56442356285344 48-49 17.7274184119454 21.771859548626324 27.10114674025907 33.39957529916921 50-51 23.081553843254422 23.41658493956596 26.515292470983375 26.986568746196237 52-53 24.456189672495398 21.511853330564414 29.99434612517511 24.03761087176508 54-55 20.449140935134093 23.455417816547968 29.882769126242266 26.21267212207568 56-57 20.298611019414036 25.870978816575573 27.888307762854165 25.942102401156227 58-59 22.837512919284034 22.755165612917246 32.087180108924116 22.320141358874604 60-61 23.995656959847885 28.702597781424323 28.033495907242838 19.268249351484954 62-63 24.86900656257615 23.690935914349197 27.234510963835607 24.205546559239043 64-65 29.437001309634276 18.97193109235022 29.595633912205287 21.995433685810216 66-67 29.424157050477938 23.85971188045956 25.119649675832505 21.596481393229993 68-69 21.457955458029883 28.392895084009854 25.895226857225857 24.253922600734406 70-71 23.35236364378428 27.698524830833904 23.09211734611197 25.856994179269844 72-73 22.94920995802208 27.99568336860503 23.84968855672541 25.205418116647483 74-75 20.813821863329878 27.046468608990743 27.387741775172646 24.751967752506733 76-77 22.103529530392276 28.123405721337196 23.71686451227227 26.056200235998254 78-79 21.73860852258609 28.77348128639457 21.230480027177013 28.25743016384233 80-81 21.06380475765764 22.307537179328666 26.25324557623308 30.37541248678062 82-83 23.54358705346693 18.884302034554658 28.13612994068833 29.435980971290082 84-85 24.50654637077655 20.160145104116527 30.093859123685412 25.23944940142151 86-87 26.077387261615954 22.750424040611865 24.436563164345294 26.735625533426884 88-89 21.67990905784358 25.123731029209285 26.54494230286763 26.651417610079502 90-91 19.010223790208833 26.746609175602632 25.77758784813044 28.4655791860581 92-93 22.897652861688936 23.475404444113668 25.480549350164516 28.146393344032884 94-95 20.500758051369832 22.25231886893694 27.85271596061254 29.39420711908069 96-97 21.13768925775787 20.724272168651126 27.599852110959993 30.538186462631007 98-99 19.598358815783314 22.150345054420047 29.75900808708168 28.492288042714964 100-101 19.438765894770707 25.102003824468195 28.68879320382639 26.770437076934712 102-103 20.232589126554366 25.724530254232302 27.146221687111442 26.896658932101886 104-105 18.899487069912382 23.528942197232602 25.84931163173708 31.722259101117935 106-107 18.4985652233672 25.221278508720594 24.336524593469953 31.94363167444225 108-109 21.01530867635708 24.836895914685307 25.742836324525214 28.404959084432395 110-111 22.08640913464222 27.07669481428752 23.172840476874995 27.664055574195263 112-113 18.836079949055016 27.246023824343034 24.380308394818833 29.537587831783117 114-115 20.045279177580326 24.428608469558995 25.27033061482209 30.25578173803859 116-117 23.38697140469964 24.484722183040894 23.85628702260386 28.27201938965561 118-119 21.0616220472819 25.281377018796082 24.65866230676337 28.998338627158642 120-121 19.659434455447446 24.7496715404916 26.05342621229918 29.537467791761774 122-123 19.262187831615137 26.4176322019005 27.47790032699353 26.84227963949083 124-125 19.149419007795593 27.52933477818435 24.261550052981207 29.05969616103885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 5.0 2 26.5 3 86.0 4 129.5 5 157.0 6 215.0 7 258.5 8 322.0 9 440.0 10 521.0 11 565.5 12 641.0 13 741.0 14 809.0 15 874.5 16 928.5 17 997.0 18 1073.5 19 1101.0 20 1163.5 21 1253.0 22 1310.0 23 1359.0 24 1451.5 25 1527.0 26 1585.0 27 1675.5 28 1777.0 29 2068.0 30 2561.0 31 2937.0 32 3655.5 33 4657.5 34 5489.0 35 6255.5 36 7513.5 37 8585.0 38 8781.0 39 9043.5 40 9011.5 41 8634.5 42 8486.0 43 9223.5 44 10320.5 45 12270.0 46 21018.0 47 41311.5 48 62695.0 49 77570.0 50 81916.0 51 76788.0 52 66931.0 53 53432.0 54 41509.0 55 33663.5 56 27717.5 57 22136.5 58 18597.0 59 14711.0 60 11143.5 61 8812.0 62 6655.0 63 4731.0 64 3505.5 65 3050.0 66 2748.5 67 2432.0 68 1914.0 69 1205.5 70 783.0 71 635.0 72 614.5 73 562.0 74 449.5 75 380.5 76 263.0 77 165.0 78 144.5 79 123.5 80 97.0 81 81.0 82 49.0 83 19.0 84 4.0 85 4.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2067085445533739 2 7.202388311964247E-4 3 0.0 4 0.11751896929021663 5 0.0319305881830415 6 0.0016805572727916578 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 6.001990259970206E-5 108-109 0.0 110-111 0.0010203383441949351 112-113 1.8005970779910618E-4 114-115 3.6011941559821236E-4 116-117 0.004261413084578846 118-119 0.0013204378571934454 120-121 1.8005970779910618E-4 122-123 0.0015605174675922537 124-125 0.025748538215272187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 833057.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.493101816354 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57930720592258 32.21819885591122 2 9.342319793270617 7.379143735931515 3 2.826881593316997 3.3492696776293553 4 1.4113264874025007 2.229506426524168 5 0.8971833703229799 1.771627709605254 6 0.5983952334346063 1.4179490328272295 7 0.40678622427648525 1.1245674840989013 8 0.32776686354155204 1.0355624091098816 9 0.26872464921658057 0.9551492938866988 >10 1.9392306417944365 15.140175761234886 >50 0.22993263075284145 6.275568738638443 >100 0.14872624558251393 11.649907636163588 >500 0.013078177014412724 3.46125579646849 >1k 0.00882016589344114 6.9007706400232935 >5k 0.0015207182574898516 5.091346801947178 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9546 1.1458999804335117 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 8754 1.0508284547155837 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8567 1.0283810111432952 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8544 1.0256200956237087 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6976 0.8373976810710431 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 4972 0.5968379114514373 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4218 0.5063278983310866 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 3948 0.47391715092724745 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2866 0.34403408170149224 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2774 0.332990419623147 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2774 0.332990419623147 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2670 0.320506279882409 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2637 0.3165449663108287 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2220 0.2664883675426772 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2028 0.24344072494439156 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1988 0.23863913273641538 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1762 0.21151013676135008 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1675 0.20106667370900191 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1657 0.19890595721541263 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1528 0.1834208223446895 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1482 0.17789899130551692 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1478 0.17741883208471929 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1429 0.17153688162994848 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1395 0.16745552825316876 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1394 0.16733548844796933 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1387 0.1664952098115735 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1339 0.16073329916200213 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1336 0.1603731797464039 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1268 0.15221047299284443 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1112 0.13348426338173738 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1068 0.1282025119529636 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1022 0.12268068091379102 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1018 0.1222005216929934 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1006 0.12076004403060055 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 990 0.11883940714741009 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 955 0.11463801396543094 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 922 0.11067670039385061 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 909 0.10911618292625835 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 907 0.10887610331585953 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 895 0.10743562565346668 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 894 0.10731558584826728 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 881 0.10575506838067504 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2003980519940412E-4 0.0 10-11 0.0 0.0 0.0 1.2003980519940412E-4 0.0 12-13 0.0 0.0 0.0 2.4007961039880824E-4 0.0 14-15 0.0 0.0 0.0 2.4007961039880824E-4 0.0 16-17 0.0 0.0 0.0 2.4007961039880824E-4 0.0 18-19 0.0 0.0 0.0 3.000995129985103E-4 0.0 20-21 0.0 0.0 0.0 0.001200398051994041 0.0 22-23 0.0 0.0 0.0 0.003661214058581826 0.0 24-25 0.0 0.0 0.0 0.007322428117163652 0.0 26-27 0.0 0.0 0.0 0.011583841201742499 0.0 28-29 0.0 0.0 0.0 0.0232877222086844 0.0 30-31 1.2003980519940412E-4 0.0 0.0 0.06848270886626005 0.0 32-33 1.2003980519940412E-4 0.0 0.0 0.15365095065523726 0.0 34-35 1.2003980519940412E-4 0.0 0.0 0.25490452634093463 0.0 36-37 1.8005970779910618E-4 0.0 0.0 0.3818466203393045 0.0 38-39 2.4007961039880824E-4 0.0 0.0 0.579372119794924 0.0 40-41 2.4007961039880824E-4 0.0 0.0 0.8872742201313956 0.0 42-43 2.4007961039880824E-4 0.0 0.0 1.547553168630718 0.0 44-45 2.4007961039880824E-4 0.0 0.0 2.484043708893869 0.0 46-47 2.4007961039880824E-4 0.0 0.0 3.4410610558461183 0.0 48-49 2.4007961039880824E-4 0.0 0.0 3.791937406443977 0.0 50-51 2.4007961039880824E-4 0.0 0.0 4.257031631689069 0.0 52-53 2.4007961039880824E-4 0.0 0.0 4.626033992872037 0.0 54-55 2.4007961039880824E-4 0.0 0.0 4.983032373535064 0.0 56-57 2.4007961039880824E-4 0.0 0.0 5.545718960407271 0.0 58-59 2.4007961039880824E-4 0.0 0.0 6.206057928809193 0.0 60-61 2.4007961039880824E-4 0.0 0.0 7.183362002840141 0.0 62-63 2.4007961039880824E-4 0.0 0.0 8.396844393600919 0.0 64-65 2.4007961039880824E-4 0.0 0.0 9.472881207408378 0.0 66-67 2.4007961039880824E-4 0.0 0.0 10.314240202051 0.0 68-69 2.4007961039880824E-4 0.0 0.0 10.96287528944598 0.0 70-71 2.4007961039880824E-4 0.0 0.0 11.718405823371029 0.0 72-73 2.4007961039880824E-4 0.0 0.0 12.46571363063992 0.0 74-75 2.4007961039880824E-4 0.0 0.0 13.260137061449576 0.0 76-77 2.4007961039880824E-4 0.0 0.0 14.187564596420174 0.0 78-79 2.4007961039880824E-4 0.0 0.0 15.436638789422572 0.0 80-81 2.4007961039880824E-4 0.0 0.0 17.89475390039337 0.0 82-83 2.4007961039880824E-4 0.0 0.0 19.614924308900832 0.0 84-85 2.4007961039880824E-4 0.0 0.0 20.4070669834117 0.0 86-87 2.4007961039880824E-4 0.0 0.0 21.09423484827569 0.0 88-89 2.4007961039880824E-4 0.0 0.0 21.884696965513765 0.0 90-91 2.4007961039880824E-4 0.0 0.0 22.889130035519777 0.0 92-93 2.4007961039880824E-4 0.0 0.0 24.42233844742917 0.0 94-95 2.4007961039880824E-4 0.0 0.0 26.687369531736724 0.0 96-97 4.801592207976165E-4 0.0 0.0 29.258081979984564 0.0 98-99 4.801592207976165E-4 0.0 0.0 31.47449694318636 0.0 100-101 4.801592207976165E-4 0.0 0.0 33.11382054289202 0.0 102-103 4.801592207976165E-4 0.0 0.0 34.415892309889955 0.0 104-105 4.801592207976165E-4 0.0 0.0 35.86879409212095 0.0 106-107 4.801592207976165E-4 0.0 0.0 37.35818797513255 0.0 108-109 4.801592207976165E-4 0.0 0.0 38.54856270339245 0.0 110-111 4.801592207976165E-4 0.0 0.0 39.664512752428706 0.0 112-113 4.801592207976165E-4 0.0 0.0 42.627515284068195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTTAG 835 0.0 88.666534 1 AGTCCGT 185 0.0 86.95126 2 GAGTAGT 1705 0.0 83.34453 1 GAGTAAG 830 0.0 81.287704 1 GAGTCCG 480 0.0 79.6092 1 GAGTAAT 335 0.0 78.421005 1 GAGTCAG 3855 0.0 78.21526 1 AGTTCGG 160 0.0 78.195755 2 AGTAGTA 1485 0.0 77.02977 2 GAGTCGT 600 0.0 76.62386 1 TCGTAGG 1015 0.0 76.306435 2 GAGTAAC 720 0.0 75.4629 1 AGTCGTA 405 0.0 72.08167 2 GAGTCGA 260 0.0 71.189 1 GAGTTGT 710 0.0 69.79821 1 GTGGTCG 240 0.0 67.170265 1 GAGTACG 510 0.0 66.73125 1 AGTACGG 340 0.0 66.58686 2 GAGTCGG 485 0.0 66.47779 1 GAGTTAC 575 0.0 66.456375 1 >>END_MODULE