##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935955_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1063171 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57634943014811 33.0 31.0 34.0 31.0 34.0 2 32.62413666286985 34.0 31.0 34.0 31.0 34.0 3 32.81592424925059 34.0 31.0 34.0 31.0 34.0 4 36.07541684263397 37.0 35.0 37.0 35.0 37.0 5 36.10771362273802 37.0 35.0 37.0 35.0 37.0 6 36.214935320846784 37.0 37.0 37.0 35.0 37.0 7 36.23995669558331 37.0 37.0 37.0 35.0 37.0 8 36.2402802559513 37.0 37.0 37.0 35.0 37.0 9 38.07257534300691 39.0 39.0 39.0 35.0 39.0 10-11 37.7845125572462 39.0 38.0 39.0 35.0 39.0 12-13 37.69279824223949 39.0 37.5 39.0 35.0 39.0 14-15 39.21469876435681 41.0 39.0 41.0 35.5 41.0 16-17 39.27633419271218 41.0 39.0 41.0 35.5 41.0 18-19 39.27936898203581 41.0 39.0 41.0 35.0 41.0 20-21 39.279974246852106 41.0 39.0 41.0 35.0 41.0 22-23 39.13886618427327 41.0 39.0 41.0 35.0 41.0 24-25 38.947748292607685 41.0 38.0 41.0 35.0 41.0 26-27 38.83930148583812 40.5 38.0 41.0 35.0 41.0 28-29 38.73371499034492 40.0 38.0 41.0 35.0 41.0 30-31 38.60837250075482 40.0 38.0 41.0 35.0 41.0 32-33 38.450009923145004 40.0 38.0 41.0 34.5 41.0 34-35 38.060788433845545 40.0 37.0 41.0 34.0 41.0 36-37 37.85346524688879 40.0 37.0 41.0 33.5 41.0 38-39 37.467631265337374 40.0 37.0 41.0 33.0 41.0 40-41 37.205416156008766 40.0 37.0 41.0 33.0 41.0 42-43 37.02758399166268 40.0 37.0 41.0 32.0 41.0 44-45 36.8656646014611 40.0 36.0 41.0 32.0 41.0 46-47 36.68324709759766 40.0 35.5 41.0 31.5 41.0 48-49 36.46718119662782 40.0 35.0 41.0 31.0 41.0 50-51 36.19886170710074 40.0 35.0 41.0 30.5 41.0 52-53 35.92087255954122 39.0 35.0 41.0 30.0 41.0 54-55 35.67006154231069 39.0 35.0 41.0 30.0 41.0 56-57 35.43020972167224 38.5 35.0 41.0 29.0 41.0 58-59 35.121746172534806 37.0 35.0 40.0 29.0 41.0 60-61 34.818168479012314 37.0 35.0 40.0 29.0 41.0 62-63 34.413180476141655 36.5 35.0 39.0 27.5 41.0 64-65 33.993572059433525 36.0 34.5 39.0 27.0 41.0 66-67 33.56137629788623 35.0 34.0 37.5 27.0 40.5 68-69 33.21819773112698 35.0 34.0 37.0 26.5 39.5 70-71 33.02406809440814 35.0 34.0 36.5 26.5 39.0 72-73 32.73076062082205 35.0 34.0 36.0 26.0 39.0 74-75 32.42924938697538 35.0 34.0 35.5 26.0 37.0 76-77 32.26954836051773 35.0 34.0 35.0 26.0 37.0 78-79 32.27567578498661 35.0 34.0 35.0 27.0 36.5 80-81 32.19218592305471 35.0 34.0 35.0 27.0 36.0 82-83 32.08588082255818 35.0 34.0 35.0 27.0 36.0 84-85 31.907607995327187 35.0 34.0 35.0 26.0 35.5 86-87 31.80955791683558 35.0 33.5 35.0 25.5 35.0 88-89 31.7591591568995 35.0 34.0 35.0 25.0 35.0 90-91 31.763049405975146 35.0 34.0 35.0 25.0 35.0 92-93 31.75000681922287 35.0 34.0 35.0 25.0 36.0 94-95 31.654505248920447 35.0 34.0 35.0 25.0 35.0 96-97 31.617640059783422 35.0 34.0 35.0 25.0 35.0 98-99 31.536410887806383 35.0 33.5 35.0 25.0 35.0 100-101 31.50417195352394 35.0 33.0 35.0 25.0 35.0 102-103 30.82116470445488 34.5 32.0 35.0 23.5 35.0 104-105 31.380406350436573 35.0 33.0 35.0 24.0 35.0 106-107 31.47499320429169 35.0 33.0 35.0 25.0 35.0 108-109 31.41234523891265 35.0 33.0 35.0 24.5 35.0 110-111 31.325403909625074 35.0 33.0 35.0 24.0 35.0 112-113 31.29290114196117 35.0 33.0 35.0 24.0 35.0 114-115 31.199255811153613 35.0 33.0 35.0 23.0 35.0 116-117 31.083582979595946 35.0 33.0 35.0 21.5 35.0 118-119 30.933507874086107 35.0 33.0 35.0 18.5 35.0 120-121 30.68681049426668 35.0 33.0 35.0 6.0 35.0 122-123 30.38060716479287 35.0 33.0 35.0 2.0 35.0 124-125 28.133122517450154 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.036724297281168106 1101 2 0.0739735393211447 1101 3 0.013894971958180236 1101 4 0.10608904596246305 1101 5 0.14088183793437992 1101 6 0.1842136742819278 1101 7 0.10987082460421504 1101 8 0.08443170019489799 1101 9 0.06563381742999752 1101 10-11 0.13413462710757074 1101 12-13 0.15965667156735464 1101 14-15 0.2294930306367604 1101 16-17 0.14133635311658566 1101 18-19 0.18755520553504113 1101 20-21 0.14504071951613184 1101 22-23 0.1541906559221431 1101 24-25 0.21765801601638657 1101 26-27 0.16274651576017618 1101 28-29 0.16140478974256212 1101 30-31 0.20834578089147016 1101 32-33 0.28848396458305814 1101 34-35 0.5075759365714845 1101 36-37 0.295198741716888 1101 38-39 0.1781805298719732 1101 40-41 0.11350306100356988 1101 42-43 0.07644216600039755 1101 44-45 0.13133024006377525 1101 46-47 0.18190320341088295 1101 48-49 0.020516225514583653 1101 50-51 0.08750437455152849 1101 52-53 0.14566813932567158 1101 54-55 0.22602662173514432 1101 56-57 0.06515483077475892 1101 58-59 0.005963920187745941 1101 60-61 -0.054415949912296924 1101 62-63 -0.10378365394658573 1101 64-65 -0.1368820636583692 1101 66-67 -0.35057258281442927 1101 68-69 -0.2040699133419892 1101 70-71 -0.002964103353534142 1101 72-73 -0.0661449022513807 1101 74-75 -0.1138958296255339 1101 76-77 -0.1265651094814899 1101 78-79 -0.14287138277348532 1101 80-81 -0.03604752369967912 1101 82-83 -0.05827794109173112 1101 84-85 -0.08217967923209102 1101 86-87 -0.2069128237700255 1101 88-89 -0.29975026447323927 1101 90-91 -0.23076418618624217 1101 92-93 -0.17056867818019583 1101 94-95 -0.3079091396527218 1101 96-97 -0.21920857274761474 1101 98-99 -0.20089730504366798 1101 100-101 -0.2920431230902345 1101 102-103 -0.43196205688538214 1101 104-105 -0.23675868478144224 1101 106-107 -0.3007012949034795 1101 108-109 -0.2853217124234817 1101 110-111 -0.3566744439058809 1101 112-113 -0.21813521047665674 1101 114-115 -0.251945087467341 1101 116-117 -0.37302966453999886 1101 118-119 -0.5285523881805396 1101 120-121 -0.42450776806309065 1101 122-123 -0.5045189474824276 1101 124-125 -0.4318222291261016 1102 1 0.062461200120743854 1102 2 0.062880305429033 1102 3 0.10574886538413608 1102 4 0.1762574532377812 1102 5 0.25611729446175957 1102 6 0.21326576862717417 1102 7 0.24807108559870983 1102 8 0.1883841790616998 1102 9 0.25203335197691956 1102 10-11 0.3007642646969657 1102 12-13 0.22652319844320346 1102 14-15 0.26922837680296396 1102 16-17 0.2088631108602641 1102 18-19 0.24852612246211692 1102 20-21 0.23154984077014973 1102 22-23 0.19403950952126792 1102 24-25 0.22783447772105347 1102 26-27 0.23690438257553836 1102 28-29 0.20552602439364165 1102 30-31 0.1459338762125455 1102 32-33 0.25457259041221647 1102 34-35 0.26571870326797153 1102 36-37 0.19566119374850643 1102 38-39 0.14036174097934406 1102 40-41 0.11681897856958301 1102 42-43 0.09007225471528812 1102 44-45 0.12881831331261395 1102 46-47 -0.01593928409119627 1102 48-49 0.04549391126018065 1102 50-51 -0.01778381331804013 1102 52-53 0.026177243154229757 1102 54-55 0.0963377240690022 1102 56-57 0.039691652682805056 1102 58-59 0.14883153905834234 1102 60-61 0.17660023445669992 1102 62-63 0.21627971659975032 1102 64-65 0.21173701798178968 1102 66-67 0.1457702512604513 1102 68-69 0.21414955436865313 1102 70-71 0.13414658309294936 1102 72-73 0.1617935123426264 1102 74-75 0.11937065775263278 1102 76-77 0.0570102923687017 1102 78-79 0.09944109036228355 1102 80-81 0.0893714131156429 1102 82-83 0.1784136149233433 1102 84-85 0.15956065835692357 1102 86-87 0.26798170386479825 1102 88-89 0.23501181039423358 1102 90-91 0.1319467585441103 1102 92-93 0.20962676758212595 1102 94-95 0.124598711479738 1102 96-97 0.1506862549921486 1102 98-99 0.142447432277379 1102 100-101 0.23602443343598267 1102 102-103 0.11205656123786056 1102 104-105 0.10776106018744258 1102 106-107 0.1701790871397897 1102 108-109 0.07840039233830964 1102 110-111 0.16586271102184114 1102 112-113 0.05797367727635461 1102 114-115 -0.008977223866004636 1102 116-117 0.19406484528214207 1102 118-119 0.26120183555446985 1102 120-121 0.20911831279872928 1102 122-123 0.31769888610841335 1102 124-125 0.0017633375688355102 1103 1 -0.05676707544749604 1103 2 0.01321235440741475 1103 3 0.010814348962306042 1103 4 0.13052407421105272 1103 5 0.06440833052867134 1103 6 0.14758212748088795 1103 7 0.140364029673691 1103 8 0.16258485334805073 1103 9 0.24604304021210055 1103 10-11 0.27307568681133887 1103 12-13 0.07149244802177179 1103 14-15 0.0710050935471287 1103 16-17 0.248766876860671 1103 18-19 0.2064225898685237 1103 20-21 0.14929709453522122 1103 22-23 0.25071684312629117 1103 24-25 0.3386784008079502 1103 26-27 0.2421708553540043 1103 28-29 0.2877618671589559 1103 30-31 0.25653443564791445 1103 32-33 0.3167993562459088 1103 34-35 0.3540143241115672 1103 36-37 0.44096527095799587 1103 38-39 0.5858114658927462 1103 40-41 0.5245829968716365 1103 42-43 0.4366593747345533 1103 44-45 0.4345349278023676 1103 46-47 0.5036546158402615 1103 48-49 0.4131830448763196 1103 50-51 0.47582320185340166 1103 52-53 0.5272664749494282 1103 54-55 0.35161068037005805 1103 56-57 0.3986141375391483 1103 58-59 0.35560890415476365 1103 60-61 0.24673689785072384 1103 62-63 0.35811510922335543 1103 64-65 0.3113447648650691 1103 66-67 0.3748282417389319 1103 68-69 0.2545592666854901 1103 70-71 0.30637510873992113 1103 72-73 0.3412321782189238 1103 74-75 0.34752767014542485 1103 76-77 0.34240152664615664 1103 78-79 0.29183429748304235 1103 80-81 0.2391586146420508 1103 82-83 0.37094598622541497 1103 84-85 0.2970118003831175 1103 86-87 0.2688296559978838 1103 88-89 0.2938613556214307 1103 90-91 0.16741301644011486 1103 92-93 0.27814486587233134 1103 94-95 0.18750809962045167 1103 96-97 0.23718362453441344 1103 98-99 0.244084753285847 1103 100-101 0.1880454400491729 1103 102-103 0.19761497777640002 1103 104-105 0.14047066321061763 1103 106-107 0.11947354094932194 1103 108-109 0.24073760961041302 1103 110-111 0.30113400013137337 1103 112-113 0.13932776096936905 1103 114-115 0.1258969494848614 1103 116-117 0.030136849431599444 1103 118-119 0.09937172237069092 1103 120-121 0.14834686623222026 1103 122-123 -0.0816337741735289 1103 124-125 0.06413135157324135 1104 1 0.05713761872036116 1104 2 0.04699613819119719 1104 3 0.1612410914943112 1104 4 0.16802407421105414 1104 5 0.21700434759837606 1104 6 0.193688101876333 1104 7 0.16502619183585665 1104 8 0.10833520896682813 1104 9 0.2204918311082622 1104 10-11 0.27362689591517864 1104 12-13 0.17653334418108813 1104 14-15 0.26454172228112327 1104 16-17 0.2806836620669344 1104 18-19 0.19795886298374654 1104 20-21 0.17751544446409184 1104 22-23 0.28701662975502273 1104 24-25 0.3176524406372607 1104 26-27 0.3096317372885764 1104 28-29 0.3706654944704795 1104 30-31 0.41012618529229883 1104 32-33 0.2793793704706573 1104 34-35 0.39116759580431193 1104 36-37 0.35272736199640065 1104 38-39 0.30744197798377826 1104 40-41 0.34809828847903646 1104 42-43 0.26937630218832 1104 44-45 0.39417219665015324 1104 46-47 0.28523356320868487 1104 48-49 0.3547193179915382 1104 50-51 0.3381009401179824 1104 52-53 0.411494782204052 1104 54-55 0.3656842934568232 1104 56-57 0.3463026154907922 1104 58-59 0.4123923323197687 1104 60-61 0.3627041809232736 1104 62-63 0.39686866541112664 1104 64-65 0.4085442669987813 1104 66-67 0.5427425376137549 1104 68-69 0.5238515853199175 1104 70-71 0.4160479394653791 1104 72-73 0.41407179415064377 1104 74-75 0.3927890499462734 1104 76-77 0.3640942720231095 1104 78-79 0.3569836573692484 1104 80-81 0.39075889913706874 1104 82-83 0.4259957728541508 1104 84-85 0.46441933950118397 1104 86-87 0.3827076787574768 1104 88-89 0.4539164765318162 1104 90-91 0.39986322981138045 1104 92-93 0.38932018592923257 1104 94-95 0.4399796501183175 1104 96-97 0.4142195420308532 1104 98-99 0.37738845172112434 1104 100-101 0.42776805740336954 1104 102-103 0.3095726591419776 1104 104-105 0.4483476191138891 1104 106-107 0.5535062578767764 1104 108-109 0.5265928727683082 1104 110-111 0.5136162191925422 1104 112-113 0.4957288989494515 1104 114-115 0.5375434644208532 1104 116-117 0.6362979447374286 1104 118-119 0.7120939841061116 1104 120-121 0.7061438079107383 1104 122-123 0.8768388431806677 1104 124-125 0.8621043245462161 1105 1 0.018626228304022163 1105 2 0.015733970767449534 1105 3 0.08163796297215953 1105 4 0.14627683216043152 1105 5 0.1067461420585687 1105 6 0.04196801239240244 1105 7 0.0185871911869242 1105 8 0.07102049534955768 1105 9 -0.03142464486428054 1105 10-11 -0.018332358157707063 1105 12-13 0.03091639175784877 1105 14-15 0.07812248835919888 1105 16-17 0.12010568809240851 1105 18-19 0.09396709413918103 1105 20-21 0.10931340546003554 1105 22-23 0.18444033787347536 1105 24-25 0.224961219967156 1105 26-27 0.21311460897134538 1105 28-29 0.14953708332256355 1105 30-31 0.0971091765146781 1105 32-33 0.1879288000959889 1105 34-35 0.17704890151830455 1105 36-37 0.2811839776595093 1105 38-39 0.23849772613091602 1105 40-41 0.2998399377840002 1105 42-43 0.2730645258298381 1105 44-45 0.31201006679599885 1105 46-47 0.41286129981170916 1105 48-49 0.24809127400096287 1105 50-51 0.28265954013848216 1105 52-53 0.3673808268094021 1105 54-55 0.3411208264668346 1105 56-57 0.34323087465264024 1105 58-59 0.36664004399025174 1105 60-61 0.3941085150876944 1105 62-63 0.32491799257393694 1105 64-65 0.32177367203310325 1105 66-67 0.40722625461229 1105 68-69 0.3420233124963943 1105 70-71 0.2576951097922304 1105 72-73 0.38958858946469377 1105 74-75 0.4329776236680587 1105 76-77 0.3857881481800831 1105 78-79 0.4788743469419785 1105 80-81 0.41402685569296693 1105 82-83 0.42777947448330167 1105 84-85 0.5283555224309282 1105 86-87 0.5428930932183604 1105 88-89 0.487336547230953 1105 90-91 0.34072229262090303 1105 92-93 0.4036830825445499 1105 94-95 0.49545200231613507 1105 96-97 0.49986646473997 1105 98-99 0.4247534573565588 1105 100-101 0.4131440948363405 1105 102-103 0.45264768731917115 1105 104-105 0.4757700037581749 1105 106-107 0.45772814045473353 1105 108-109 0.37691541622949387 1105 110-111 0.3321747423661492 1105 112-113 0.3329846966201373 1105 114-115 0.3728042315235527 1105 116-117 0.3975566540512716 1105 118-119 0.38764407973779313 1105 120-121 0.5371760750646715 1105 122-123 0.44286462104193447 1105 124-125 0.5189748631575064 1106 1 -0.02806611642395751 1106 2 0.0345767833524917 1106 3 0.07251443614626396 1106 4 0.10874988066266411 1106 5 0.08418601143538496 1106 6 0.12493173515307632 1106 7 0.07768748215843857 1106 8 0.11561449589382278 1106 9 0.11154870716938348 1106 10-11 0.16555389082180483 1106 12-13 0.09280456659195835 1106 14-15 0.026042146729338356 1106 16-17 0.026766005021087835 1106 18-19 0.1339945166339973 1106 20-21 0.1704653595744503 1106 22-23 0.12478615437301954 1106 24-25 0.0984015917408243 1106 26-27 0.1669746914481678 1106 28-29 0.22697067273873728 1106 30-31 0.13656930923118438 1106 32-33 0.13129150968968162 1106 34-35 0.3163225194036343 1106 36-37 0.32945698848197225 1106 38-39 0.29293221567478156 1106 40-41 0.3303414973075576 1106 42-43 0.2727251986229575 1106 44-45 0.25139630530890145 1106 46-47 0.2572836480983298 1106 48-49 0.30212593938372834 1106 50-51 0.3073772559074541 1106 52-53 0.37197440868961706 1106 54-55 0.3085853970221919 1106 56-57 0.30005049324969946 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2216 26-27 -0.5346655781102143 2216 28-29 -0.7872714508886745 2216 30-31 -0.7079865009833028 2216 32-33 -0.8522969006684846 2216 34-35 -0.8600627301431061 2216 36-37 -0.7013780267405494 2216 38-39 -0.9019321499394266 2216 40-41 -0.6152532073070915 2216 42-43 -0.5706938825524261 2216 44-45 -0.5958024084734674 2216 46-47 -0.664412722441817 2216 48-49 -0.7934995523550583 2216 50-51 -0.9509154270193676 2216 52-53 -0.8689560466177397 2216 54-55 -1.135450452873478 2216 56-57 -0.8408276239466161 2216 58-59 -0.5986303695834536 2216 60-61 -0.5624527929004728 2216 62-63 -0.6352757365534885 2216 64-65 -0.7036770172563962 2216 66-67 -0.8055631915819745 2216 68-69 -0.6719959337960049 2216 70-71 -0.7166326153149996 2216 72-73 -0.609287654975887 2216 74-75 -0.644854319479407 2216 76-77 -0.6845731946286691 2216 78-79 -0.63531346268433 2216 80-81 -0.6138296201462907 2216 82-83 -0.6215470635581966 2216 84-85 -0.7494020312640117 2216 86-87 -0.9418887241001421 2216 88-89 -0.747678726348127 2216 90-91 -0.6951310493648251 2216 92-93 -0.906259780024893 2216 94-95 -0.9882574989579815 2216 96-97 -0.9015437693493986 2216 98-99 -1.027079372133315 2216 100-101 -0.6984123857511761 2216 102-103 -1.0514793445416757 2216 104-105 -0.9328852881808594 2216 106-107 -0.9578160896144325 2216 108-109 -0.9720937795730435 2216 110-111 -0.9231630965411206 2216 112-113 -0.7025164825318235 2216 114-115 -0.7751595550578543 2216 116-117 -0.8478968841959862 2216 118-119 -0.719979256391742 2216 120-121 -1.018426083812205 2216 122-123 -0.9820099456790707 2216 124-125 -1.548062695602244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 16.0 4 61.0 5 128.0 6 342.0 7 699.0 8 1059.0 9 1722.0 10 4016.0 11 12867.0 12 18668.0 13 5798.0 14 4755.0 15 6080.0 16 3710.0 17 2353.0 18 1945.0 19 1904.0 20 1895.0 21 1996.0 22 2253.0 23 2582.0 24 3131.0 25 4330.0 26 5547.0 27 7439.0 28 9516.0 29 12388.0 30 15816.0 31 20764.0 32 28330.0 33 38750.0 34 69102.0 35 108103.0 36 212445.0 37 384278.0 38 68132.0 39 245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 68.92143454323868 10.420222878455554 7.052068124686009 13.606274453619772 2 11.364602615618463 29.983765256520233 45.25975589567625 13.391876232185048 3 36.22098420667983 20.468767488955212 20.131662733464324 23.178585570900637 4 15.220519407628952 16.197634056626693 46.21603500134664 22.36581153439771 5 16.80356017198717 32.96179212885866 28.90870943765465 21.325938261499523 6 30.61925526831636 38.01493301026762 15.722552269298347 15.643259452117672 7 44.10551077860475 22.513405651583803 17.92740772650872 15.453675843302724 8 42.611395532797644 22.839881825219084 16.883737423236713 17.664985218746562 9 52.32187484421603 9.654890887731137 14.840039843073221 23.183194424979614 10-11 35.9249829049137 24.62510734397383 23.837134383838535 15.612775367273937 12-13 28.626862470853702 27.139848622658068 27.003746339958486 17.229542566529748 14-15 24.53631635926864 26.617637238036025 26.26614157082915 22.57990483186618 16-17 22.2911460150813 31.949611116179806 25.034589920153955 20.72465294858494 18-19 22.96883568118393 25.01135753326605 28.15995733517938 23.85984945037064 20-21 23.174823241040247 25.72746999306791 26.406899736730967 24.690807029160876 22-23 23.728403050873283 23.300626145747017 26.506601478031282 26.464369325348414 24-25 20.286482607219348 22.2684309485492 36.87388952482715 20.57119691940431 26-27 17.867398565235508 22.20437728267607 34.49233472320069 25.43588942888773 28-29 21.479376318579043 22.914470014701305 27.00943686387232 28.596716802847332 30-31 21.59097642806284 26.598167181008513 26.237030543534388 25.573825847394254 32-33 28.995805942788135 22.532781650364804 24.20255067152885 24.26886173531821 34-35 26.83246627306426 18.09412596844722 30.507839284555356 24.56556847393317 36-37 24.067906291650168 24.73943514260641 31.16323714623518 20.029421419508246 38-39 22.476440760705472 28.742318968444398 26.55348951391639 22.22775075693374 40-41 20.394367415966013 27.091408625705554 28.89897297800636 23.615250980322074 42-43 21.67713378186576 22.08967325105745 25.604441806633176 30.628751160443617 44-45 22.998840261820536 23.499606366238357 24.98864246673395 28.512910905207157 46-47 23.89601484615363 22.08882672683886 25.21969654928511 28.7954618777224 48-49 18.18108281734547 22.686613912531474 26.97049674981729 32.16180652030577 50-51 23.174399978930953 23.967452084377772 26.74452181257766 26.11362612411362 52-53 23.77665493133278 21.85368111056453 30.01248152931184 24.357182428790853 54-55 21.11071502138414 24.121425433914208 28.82889958435661 25.93895996034504 56-57 20.931157828797062 25.931294213254503 27.709559421767523 25.42798853618092 58-59 23.42774586590492 22.39004826128628 31.343593833917595 22.838612038891203 60-61 25.236250800670824 28.223634768066475 27.57096459553543 18.969149835727272 62-63 26.203263633037395 24.104589007788963 26.572113046725317 23.120034312448325 64-65 29.788246669632635 19.876623798053185 28.29648287998826 22.03864665232592 66-67 29.502403658489555 24.357605690900147 24.37397182579284 21.766018824817458 68-69 22.868851765144083 28.554813854027245 25.620807941525868 22.955526439302805 70-71 24.51425970046211 27.09855705244029 23.72478180838266 24.66240143871494 72-73 22.890908423950616 27.877500420910657 24.668044933505524 24.56354622163321 74-75 20.947053672457205 27.27294104146934 26.77245711179105 25.007548174282405 76-77 22.660136516138984 28.53092305941377 23.404983770249565 25.40395665419768 78-79 23.08278724683047 28.617738820942257 20.902987383967396 27.396486548259873 80-81 22.349132924054548 22.484953032014605 25.466834591989436 29.699079451941408 82-83 24.393535941066865 19.02238680325178 28.49019583867506 28.093881417006294 84-85 25.785597989410924 20.793033293797517 30.869728388001555 22.551640328790008 86-87 27.14695002026955 23.503933045577803 24.805793235519026 24.543323698633614 88-89 22.306054247153092 25.414632265176536 27.607177020441682 24.672136467228693 90-91 19.749221903155746 26.788352955451195 27.111678177828402 26.35074696356466 92-93 24.129326326621023 22.47676996456826 27.139801593534813 26.254102115275906 94-95 22.173479148697623 22.120336239419622 28.9394650531288 26.766719558753955 96-97 21.68653960651673 20.81588944769938 28.92385138420818 28.5737195615757 98-99 19.65977251072499 23.740630622919547 29.802496493978865 26.797100372376597 100-101 19.352296102884672 27.246416615953596 27.462374349939942 25.938912931221786 102-103 21.22711210143994 26.103185658751038 26.573194716560177 26.096507523248846 104-105 20.607315286064047 22.985578049062664 25.455970864517564 30.95113580035573 106-107 20.150530888507532 25.243458128305857 23.902139873143582 30.70387111004303 108-109 22.584795860684686 24.461963315402695 26.35465038079481 26.598590443117804 110-111 24.140434969910284 26.69368689766487 23.349204348570385 25.816673783854455 112-113 20.691818516153123 26.700881985836684 25.258543091455827 27.34875640655437 114-115 21.348950299108317 23.595366642838332 26.951775838067647 28.1039072199857 116-117 24.951686947409907 22.73255521374146 25.085726567640386 27.230031271208244 118-119 21.156548583167318 24.487511592951485 26.096650883321765 28.259288940559436 120-121 19.083746525120947 24.33193671922507 28.400584480417695 28.183732275236288 122-123 19.736207196572433 26.015218996468025 29.44048083751511 24.80809296944443 124-125 20.593815344737962 26.706872178930208 24.721027216340623 27.978285259991203 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 5.5 2 32.0 3 90.0 4 163.5 5 230.5 6 299.0 7 398.5 8 538.0 9 705.5 10 891.0 11 989.0 12 1102.0 13 1266.0 14 1421.0 15 1577.0 16 1703.5 17 1763.5 18 1865.0 19 2006.5 20 2096.0 21 2234.0 22 2355.5 23 2485.5 24 2604.5 25 2732.5 26 3035.5 27 3382.0 28 3588.0 29 3831.0 30 4151.0 31 4532.0 32 5194.0 33 5914.0 34 6725.5 35 7420.5 36 8160.0 37 9173.0 38 9825.5 39 10313.0 40 10665.0 41 11477.0 42 12291.5 43 13178.0 44 14621.5 45 17962.0 46 33660.5 47 64933.0 48 91082.5 49 105874.0 50 104670.5 51 84869.0 52 63685.5 53 48529.5 54 39786.0 55 34730.0 56 30510.5 57 26478.5 58 23136.0 59 19482.0 60 16989.0 61 14754.0 62 11331.5 63 8645.0 64 7084.0 65 6435.0 66 5785.5 67 5094.5 68 4460.5 69 3612.5 70 2777.0 71 1964.0 72 1451.0 73 1125.5 74 796.5 75 640.0 76 470.5 77 318.5 78 235.0 79 173.5 80 165.0 81 181.0 82 161.0 83 70.0 84 11.5 85 5.5 86 5.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2101261227027449 2 0.0017871066836849389 3 0.0 4 0.11935991482085195 5 0.02831153219942982 6 0.0021633396697238734 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 4.702912325486681E-5 108-109 0.0 110-111 0.0015989901906654715 112-113 1.8811649301946724E-4 114-115 0.0010346407116070698 116-117 0.004938057941761015 118-119 0.0023514561627433405 120-121 4.2326210929380126E-4 122-123 0.002445514409253074 124-125 0.028076386583155484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1063171.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.046090925499044 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15349656690665 36.18540379633644 2 9.521004864614033 8.387260919378168 3 2.82519895856706 3.733169106350097 4 1.3876192766514621 2.44476819317462 5 0.820934160589051 1.8079470340576786 6 0.5329522800856212 1.408467875256198 7 0.40489966482249223 1.2483973216732904 8 0.29006411336846366 1.022095224932129 9 0.21931138846038464 0.8693828421411189 >10 1.5474322191493088 13.118658614776216 >50 0.15913234358497017 4.910812850603792 >100 0.11998803963664155 10.875992040618714 >500 0.00962470905209966 2.798414380499063 >1k 0.006844237548159758 5.2504301857719 >5k 8.555296935199697E-4 2.7604490212887915 >10k+ 6.416472701399774E-4 3.1783505931418077 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12267 1.1538125099349024 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11360 1.068501680350574 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 10111 0.9510229304599166 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 9695 0.9118946999118674 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8106 0.7624361462079007 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6003 0.5646316537979309 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5498 0.5171322393105154 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3308 0.3111446794541988 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3076 0.2893231662639406 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3048 0.2866895353616681 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3022 0.284244020952415 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2805 0.2638333814598028 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2426 0.22818530603261378 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2299 0.2162399087258776 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2037 0.1915966481403274 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1815 0.17071571741516653 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1794 0.1687404942384621 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1720 0.16178018399674182 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1677 0.1577356793968233 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1665 0.15660698043870647 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1660 0.1561366892061578 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1660 0.1561366892061578 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1653 0.15547828148058968 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1638 0.15406740778294367 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1465 0.13779533113675974 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1450 0.13638445743911373 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1327 0.12481529311841652 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1315 0.12368659416029971 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1310 0.12321630292775104 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1278 0.12020643903943957 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1254 0.11794904112320595 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1222 0.11493917723489448 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1202 0.1130580123046998 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1171 0.11014220666289806 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1163 0.10938974069082019 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1135 0.10675610978854765 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1081 0.10167696447702203 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.702912325486681E-5 0.0 20-21 0.0 0.0 0.0 7.52465972077869E-4 0.0 22-23 0.0 0.0 0.0 0.0018341358069398055 0.0 24-25 0.0 0.0 0.0 0.003621242490624744 0.0 26-27 0.0 0.0 0.0 0.004938057941761015 0.0 28-29 0.0 0.0 0.0 0.010769669225364499 0.0 30-31 0.0 0.0 0.0 0.031227337841231564 0.0 32-33 0.0 0.0 0.0 0.07675152915194264 0.0 34-35 0.0 0.0 0.0 0.13074096264852975 0.0 36-37 0.0 0.0 0.0 0.2020841426261627 0.0 38-39 0.0 0.0 0.0 0.3142015724657652 0.0 40-41 0.0 0.0 0.0 0.48703360042740074 0.0 42-43 0.0 0.0 0.0 0.8595983148524555 0.0 44-45 0.0 0.0 0.0 1.3843963012535143 0.0 46-47 0.0 0.0 0.0 1.9093353750243376 0.0 48-49 0.0 0.0 0.0 2.132253419252406 0.0 50-51 0.0 0.0 0.0 2.4193662167233683 0.0 52-53 0.0 0.0 0.0 2.6600612695417762 0.0 54-55 0.0 0.0 0.0 2.8966177595137568 0.0 56-57 0.0 0.0 0.0 3.2635389791482274 0.0 58-59 0.0 0.0 0.0 3.6721280019865103 0.0 60-61 0.0 0.0 0.0 4.243061558300593 0.0 62-63 0.0 0.0 0.0 4.923761088291535 0.0 64-65 0.0 0.0 0.0 5.554280543769535 0.0 66-67 0.0 0.0 0.0 6.0837344133728255 0.0 68-69 0.0 0.0 0.0 6.538035744014839 0.0 70-71 0.0 0.0 0.0 7.0474081779883 0.0 72-73 0.0 0.0 0.0 7.535946710359857 0.0 74-75 0.0 0.0 0.0 8.021005087610554 0.0 76-77 0.0 0.0 0.0 8.575807654648218 0.0 78-79 0.0 0.0 0.0 9.301608113840576 0.0 80-81 0.0 0.0 0.0 11.173696423247058 0.0 82-83 0.0 0.0 0.0 12.45232422630038 0.0 84-85 0.0 0.0 0.0 13.040752616465273 0.0 86-87 0.0 0.0 0.0 13.531219342890278 0.0 88-89 0.0 0.0 0.0 14.11122951999255 9.405824650973362E-5 90-91 0.0 0.0 0.0 14.814644116515595 1.8811649301946724E-4 92-93 0.0 0.0 0.0 15.693712488395564 1.8811649301946724E-4 94-95 0.0 0.0 0.0 16.781778284020163 1.8811649301946724E-4 96-97 0.0 0.0 0.0 17.97001611217763 1.8811649301946724E-4 98-99 0.0 0.0 0.0 19.19009265677864 1.8811649301946724E-4 100-101 0.0 0.0 0.0 20.33181868203704 1.8811649301946724E-4 102-103 0.0 0.0 0.0 21.38197900431821 1.8811649301946724E-4 104-105 0.0 0.0 0.0 22.4936063906935 1.8811649301946724E-4 106-107 0.0 0.0 0.0 23.545318674042086 1.8811649301946724E-4 108-109 0.0 0.0 0.0 24.411312949657205 1.8811649301946724E-4 110-111 0.0 0.0 0.0 25.403345275595363 1.8811649301946724E-4 112-113 0.0 0.0 0.0 28.33753930458976 1.8811649301946724E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 1725 0.0 85.48009 1 GAGTACG 460 0.0 84.35535 1 GAGTAAG 1050 0.0 83.57668 1 AGTAGTA 1485 0.0 81.441605 2 GAGTCCG 475 0.0 79.17791 1 GAGTTAG 1010 0.0 77.4296 1 AGTCCGT 255 0.0 77.09931 2 AGTCGAG 110 0.0 75.82494 2 TCGTAGG 985 0.0 75.605 2 GAGTCGT 630 0.0 73.911354 1 GAGTCAG 4215 0.0 73.081795 1 GAGTTAA 385 0.0 72.877625 1 AGTAAGG 595 0.0 72.092865 2 GAGTCGG 615 0.0 70.8606 1 AGTACGG 295 0.0 70.68427 2 GAGTTAT 475 0.0 69.12357 1 GAGTATA 415 0.0 69.04787 1 GTGTTCG 240 0.0 67.159836 1 TGTTCGG 240 0.0 67.023834 2 TAAGCGG 160 0.0 67.02068 4 >>END_MODULE