FastQCFastQC Report
Thu 26 May 2016
SRR935954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902815
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT187820.6470271098916052No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT173400.5973511918603149No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA117810.4058474274109786No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC105030.36182119769947446No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT101670.35024622650771753No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT85330.29395603922399466No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC81440.2805552541240141No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG79960.27545675490859733No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT77680.2676023101713337No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT77230.2660520908152948No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT69450.23925052061533375No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA65080.22419616820224506No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC63830.2198900033243593No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA61900.21324128475290363No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT61540.21200110926807253No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA60450.20824613349455617No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA59150.20376772202155494No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG54920.18919566007478947No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA54460.1876109913997275No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT52570.1811000701043642No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA50820.17507143927532412No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG50020.1723154937534772No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC49670.17110976758766921No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA49360.17004183869795353No Hit
GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC48930.16856051797996083No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT47340.16308307625529012No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA46980.16184290077045901No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT46350.1596725936720046No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG45410.15643435768383449No Hit
GACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC44980.15495303696584178No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG43620.15026792957870205No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC42760.14730528814271665No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC41830.14410150147356962No Hit
ACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAAT40300.13883075566303743No Hit
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG39490.13604036082216744No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA39190.13500688125147486No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG38720.1333877632573898No Hit
GAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGA37880.13049402045945058No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA36740.12656679809081875No Hit
ACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTAC36600.12608450762449552No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC36190.124672085544549No Hit
GGTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCG35160.12112380568517112No Hit
GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA34900.12022812339057087No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA34260.11802336697309336No Hit
AGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG33960.11698988740240077No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT31140.10727517943789047No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA30180.1039680448116742No Hit
GGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCT29720.1023833761366122No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT29580.10190108567028902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207500.060.2706531
GTATACG2700.048.5478971
GTATCAA340650.046.57731
AGTAAGG14100.040.9315032
TCTACGG1900.040.709412
GAGTAAG23350.039.8060651
TAGACGG4850.039.255984
TATCAAC421750.037.6246952
GTGGTAT83900.037.4958721
GTAAGGG16500.035.337823
TGGTATC90500.034.515632
GAGTTAG20450.034.3795551
TAGATAG3850.033.9937975
GTAGGGG33000.032.2728043
GAGTAGG35900.032.0312841
GTCTAGG4700.031.6922951
GATAGTC3950.031.627147
ATCAACG503400.031.521543
AGAGTCG41550.031.4984665
AACGCAG503100.031.4177446
Produced by FastQC (version 0.11.5)