FastQCFastQC Report
Thu 26 May 2016
SRR935954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902815
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT199060.6857481444735541No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT173910.5991081071304922No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA117130.4035048737174088No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC113110.38965624747012817No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT110820.3817673534138414No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG84060.2895809757080627No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC79230.27294195461991205No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT79160.27270080938675045No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT78170.26929032680346493No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT74090.25523500464204574No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT73750.2540637277952608No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA67580.23280849795801664No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA66480.22901907286547712No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC65990.2273310562333459No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG64060.22068233766189027No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA63870.22002780060045163No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA61270.2110709776544492No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT60660.20896956919404094No Hit
GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC59330.20438780976397047No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA57140.1968434088979146No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA55450.19102147398301306No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA52710.1815823605706874No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT52390.18047998236194865No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG51510.17744844228791706No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT50350.17345232128123908No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT50110.172625537624685No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG49360.17004183869795353No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA47550.1638065119547749No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG46350.1596725936720046No Hit
GACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC45040.1551597328799803No Hit
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG43540.14999233502651738No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC43230.1489244061368017No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC42940.1479253758851322No Hit
GGTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCG42620.14682299767639345No Hit
ACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTAC42450.14623735925300096No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG42390.14603066333886244No Hit
ACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAAT41150.14175894777999976No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA39470.1359714621841213No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC38570.13287102347204352No Hit
AGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTG38380.13221648641060488No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA36840.1269112912810496No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC36670.12632565285765712No Hit
GAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGA36360.12525772396794146No Hit
TATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCA35850.12350080869776407No Hit
GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA34040.11726548195458546No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA32430.11171914159186858No Hit
AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG31340.10796416581835219No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT31250.10765412194714441No Hit
GGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCT31240.10761967262812133No Hit
GAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTG30500.10507042302041293No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT30190.10400249413069727No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCG29770.10255562273172765No Hit
ACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA29610.10200443362735827No Hit
AAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAG29100.10024751835718086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221000.063.5489541
GTATACG2100.048.338481
GTGTACG3750.046.177461
GTATCAA353800.044.775741
GTGGTAT88550.039.246221
TGTACGG4000.038.7306022
TACGGGA4850.038.08564
GTCTAGG4500.037.1542851
TGGTATC94600.036.6583372
TATCAAC443750.035.6237642
CGTATAG1407.824201E-834.1212771
GTACTAG3850.032.5703161
GAGTAAG23850.032.2971841
CGTATCA953.259964E-431.360812
AGAGTCG40850.030.8864545
ATAAGGG1951.4006218E-930.5566863
AGTAAGG13500.030.4548322
ATCAACG526550.030.0219213
AACGCAG536550.029.5180346
TCAACGC537100.029.4322174
Produced by FastQC (version 0.11.5)