##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1233309 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.018477932132175 33.0 31.0 34.0 28.0 34.0 2 30.80868703625774 31.0 31.0 34.0 27.0 34.0 3 31.04555387173855 33.0 31.0 34.0 27.0 34.0 4 34.23484463342115 37.0 35.0 37.0 30.0 37.0 5 34.299991324153154 37.0 35.0 37.0 31.0 37.0 6 34.4928845893446 37.0 35.0 37.0 31.0 37.0 7 34.435102638511516 37.0 35.0 37.0 31.0 37.0 8 34.35064043155446 37.0 35.0 37.0 31.0 37.0 9 35.936273877835966 39.0 37.0 39.0 31.0 39.0 10-11 35.69673131388808 39.0 37.0 39.0 30.0 39.0 12-13 35.529316659490846 39.0 37.0 39.0 29.5 39.0 14-15 36.975637086893876 40.0 38.0 41.0 30.0 41.0 16-17 37.13721135579162 40.0 38.0 41.0 30.0 41.0 18-19 37.20181398173531 40.0 38.0 41.0 30.0 41.0 20-21 37.33900709392374 40.0 38.0 41.0 30.0 41.0 22-23 37.28750702378723 40.0 37.5 41.0 30.0 41.0 24-25 37.2533801342567 40.0 37.0 41.0 30.0 41.0 26-27 37.22717461722894 40.0 37.0 41.0 30.0 41.0 28-29 37.13131380700214 40.0 37.0 41.0 30.0 41.0 30-31 37.048623662034416 40.0 37.0 41.0 30.0 41.0 32-33 36.9723187781813 40.0 36.0 41.0 30.0 41.0 34-35 36.73522410036739 40.0 36.0 41.0 30.0 41.0 36-37 36.72861910518775 40.0 36.0 41.0 30.0 41.0 38-39 36.84403584178823 40.0 36.0 41.0 30.0 41.0 40-41 36.80493696226979 40.0 35.5 41.0 30.0 41.0 42-43 36.65644741098946 40.0 35.0 41.0 30.0 41.0 44-45 36.47859701015723 40.0 35.0 41.0 30.0 41.0 46-47 36.30815270139114 40.0 35.0 41.0 30.0 41.0 48-49 36.124045555493396 39.0 35.0 41.0 30.0 41.0 50-51 35.86705440404635 39.0 35.0 41.0 29.0 41.0 52-53 35.6080171311488 38.5 35.0 41.0 29.0 41.0 54-55 35.342243914542095 38.0 35.0 40.5 28.5 41.0 56-57 35.053408756443034 37.5 34.0 40.0 28.0 41.0 58-59 34.67118175574815 37.0 34.0 40.0 27.5 41.0 60-61 34.37755136790537 36.5 34.0 39.0 27.0 41.0 62-63 33.96574945938123 36.0 34.0 39.0 26.5 41.0 64-65 33.55553879846819 35.5 33.0 38.5 26.0 40.5 66-67 33.250154665213664 35.0 33.0 37.0 26.0 40.0 68-69 33.052736175605624 35.0 33.0 37.0 26.5 39.5 70-71 32.86817942624273 35.0 33.0 36.5 26.0 39.0 72-73 32.603332984677806 35.0 33.0 36.0 26.0 39.0 74-75 32.28793635658217 35.0 33.0 35.5 26.0 37.5 76-77 32.0692174467226 35.0 33.0 35.0 25.5 37.0 78-79 31.861951465528914 35.0 33.0 35.0 25.0 37.0 80-81 31.67529143142554 35.0 33.0 35.0 25.0 36.0 82-83 31.572182640360204 35.0 33.0 35.0 25.0 36.0 84-85 31.32132863702446 35.0 33.0 35.0 24.0 35.5 86-87 31.117083796518145 35.0 32.0 35.0 23.0 35.0 88-89 30.97459476903193 35.0 32.0 35.0 22.5 35.0 90-91 30.88800333087653 35.0 32.0 35.0 20.0 35.0 92-93 30.755963428467645 35.0 32.0 35.0 19.0 35.5 94-95 30.562054602698918 35.0 32.0 35.0 18.0 35.0 96-97 30.415253192833262 35.0 32.0 35.0 17.0 35.0 98-99 30.2398308128782 35.0 31.0 35.0 15.0 35.0 100-101 30.118506797566546 35.0 31.0 35.0 9.0 35.0 102-103 29.38678790149103 34.0 29.5 35.0 7.5 35.0 104-105 29.879401674681688 35.0 31.0 35.0 6.0 35.0 106-107 29.861964844171247 35.0 31.0 35.0 2.0 35.0 108-109 29.70131735031529 35.0 31.0 35.0 2.0 35.0 110-111 29.544095599723995 35.0 31.0 35.0 2.0 35.0 112-113 29.40010492098898 35.0 30.0 35.0 2.0 35.0 114-115 29.12370622447416 35.0 30.0 35.0 2.0 35.0 116-117 28.948745204972965 35.0 30.0 35.0 2.0 35.0 118-119 28.74836435962115 35.0 30.0 35.0 2.0 35.0 120-121 28.506147283446403 35.0 30.0 35.0 2.0 35.0 122-123 28.057700868152263 34.5 29.0 35.0 2.0 35.0 124-125 25.317385180842756 31.5 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.251715103770092 1101 2 0.38796263696053757 1101 3 0.39522959271496916 1101 4 0.36405466458791835 1101 5 0.30153318067451806 1101 6 0.17016557122092024 1101 7 0.12730468300213715 1101 8 0.08766936931866098 1101 9 0.18732268245111072 1101 10-11 0.17962491658827417 1101 12-13 0.10635023040943281 1101 14-15 0.27226429549495634 1101 16-17 0.3334119975663299 1101 18-19 0.30166423941957987 1101 20-21 0.1319625601390868 1101 22-23 0.18826509808894087 1101 24-25 0.20344765008861287 1101 26-27 0.27078859273348144 1101 28-29 0.27915186748360554 1101 30-31 0.2654554846705679 1101 32-33 0.22624363205948583 1101 34-35 0.05674056947604811 1101 36-37 -0.04036139970070707 1101 38-39 0.1202174583464668 1101 40-41 0.06003931718002775 1101 42-43 -0.03108489378100643 1101 44-45 0.030639706773150976 1101 46-47 0.026542484420090773 1101 48-49 -0.04955751646021156 1101 50-51 0.07781962555218058 1101 52-53 0.08947194944590109 1101 54-55 0.020921260644854556 1101 56-57 -0.07232143067284369 1101 58-59 -0.1010967596714778 1101 60-61 -0.07026534259870942 1101 62-63 0.01049920034368057 1101 64-65 -0.023138511879942314 1101 66-67 -0.20393789826534459 1101 68-69 -0.195957034933663 1101 70-71 -0.2981297256560609 1101 72-73 -0.13216072327814743 1101 74-75 -0.1723456680929445 1101 76-77 -0.024606744107977363 1101 78-79 0.03836349397216665 1101 80-81 -0.026421321427875455 1101 82-83 -0.04981175856053355 1101 84-85 -0.11319719472058054 1101 86-87 -0.2155197731195173 1101 88-89 -0.14005732586797848 1101 90-91 -0.19572951756424928 1101 92-93 -0.4079196173879822 1101 94-95 -0.2962367862386053 1101 96-97 -0.27752510340238956 1101 98-99 -0.3082871508884999 1101 100-101 -0.1416702326958621 1101 102-103 -0.093972337371941 1101 104-105 -0.32116696372454 1101 106-107 -0.19894225402825683 1101 108-109 -0.0574442739983283 1101 110-111 -0.03273386328898198 1101 112-113 0.0853962703025104 1101 114-115 -0.025520877921024976 1101 116-117 -0.04434452187023652 1101 118-119 0.11312410284978824 1101 120-121 -0.031347745914356295 1101 122-123 0.044066173635158634 1101 124-125 0.10966716441575386 1102 1 0.6251563533883129 1102 2 0.5316392807931649 1102 3 0.4624976526943172 1102 4 0.7613600298598158 1102 5 0.5733727751885311 1102 6 0.6452321099006184 1102 7 0.6130065024532527 1102 8 0.6573877252678741 1102 9 0.6639294279461154 1102 10-11 0.8570926653265829 1102 12-13 0.8324917505455574 1102 14-15 0.9345179569403328 1102 16-17 1.001351989130825 1102 18-19 1.0210609917502467 1102 20-21 0.9714472671507295 1102 22-23 1.030177557911216 1102 24-25 0.9919888168061846 1102 26-27 0.9476102254399663 1102 28-29 0.9952671357471985 1102 30-31 0.9473824157466879 1102 32-33 1.013075518267442 1102 34-35 0.9961449210329789 1102 36-37 0.9169482196835048 1102 38-39 0.8898585494353668 1102 40-41 0.9966972211025364 1102 42-43 0.8363693290617604 1102 44-45 0.892676117611316 1102 46-47 0.9127860668916981 1102 48-49 0.870638427225515 1102 50-51 0.769029066045519 1102 52-53 0.9418075298231727 1102 54-55 0.9688976485035425 1102 56-57 0.9324557192900684 1102 58-59 0.9437913971707417 1102 60-61 0.9271461936812315 1102 62-63 0.9832866983438819 1102 64-65 1.0351890038639127 1102 66-67 0.9553687905081603 1102 68-69 1.1461801292823424 1102 70-71 1.0824097103286405 1102 72-73 1.0683001388886026 1102 74-75 1.1172259391615924 1102 76-77 1.0888069810400012 1102 78-79 1.1075953540009635 1102 80-81 1.0260591532874273 1102 82-83 1.0573520574872823 1102 84-85 1.1969374097560568 1102 86-87 1.0713201122739662 1102 88-89 0.991903238147323 1102 90-91 1.0701779536750387 1102 92-93 1.1167758681625806 1102 94-95 1.0796613041376482 1102 96-97 0.9327627581971569 1102 98-99 1.0156511390310712 1102 100-101 1.1011997659951795 1102 102-103 1.2096563840673298 1102 104-105 0.9365946096425368 1102 106-107 0.748597814619302 1102 108-109 0.8601552067818083 1102 110-111 0.9503362241203028 1102 112-113 0.7881458085313469 1102 114-115 0.9037537777503992 1102 116-117 0.8069386202098485 1102 118-119 0.6066256735989484 1102 120-121 0.5000028802037129 1102 122-123 0.4999255770413562 1102 124-125 0.6040270114131516 1103 1 0.3999862825375651 1103 2 0.3025302901776712 1103 3 0.36911052758000196 1103 4 0.4083829425574734 1103 5 0.5292013941315972 1103 6 0.3584090209407975 1103 7 0.41456629807768763 1103 8 0.28973065617282856 1103 9 0.4454376731137799 1103 10-11 0.49898007475702144 1103 12-13 0.5302403611320798 1103 14-15 0.5427908644497421 1103 16-17 0.6038918798593471 1103 18-19 0.6607518688689566 1103 20-21 0.5903251315838247 1103 22-23 0.7292998442832683 1103 24-25 0.63115689121814 1103 26-27 0.672353303276175 1103 28-29 0.6338537802219832 1103 30-31 0.6023634129145918 1103 32-33 0.6173349681468636 1103 34-35 0.6153959936172981 1103 36-37 0.635836183910861 1103 38-39 0.661726852268707 1103 40-41 0.6509361255100643 1103 42-43 0.5565106016635113 1103 44-45 0.4853387900168826 1103 46-47 0.4873950111588172 1103 48-49 0.48441693320307877 1103 50-51 0.429771269488576 1103 52-53 0.533808008525746 1103 54-55 0.5528400824432751 1103 56-57 0.501649340647397 1103 58-59 0.5126206304026653 1103 60-61 0.4877293945412333 1103 62-63 0.29454367999961306 1103 64-65 0.36572742666344027 1103 66-67 0.3813558032795541 1103 68-69 0.5129643617049027 1103 70-71 0.43347855910428734 1103 72-73 0.5100999014742627 1103 74-75 0.43590938255501044 1103 76-77 0.4869920618452781 1103 78-79 0.5166909796832186 1103 80-81 0.46777538504035476 1103 82-83 0.4906026209312877 1103 84-85 0.48831941441303073 1103 86-87 0.4942306654521538 1103 88-89 0.5300455262626329 1103 90-91 0.38781838578021066 1103 92-93 0.45367352957596907 1103 94-95 0.5683931158608821 1103 96-97 0.25551136878367586 1103 98-99 0.40963062399690386 1103 100-101 0.5353945910266198 1103 102-103 0.4462871272876683 1103 104-105 0.5303545970247079 1103 106-107 0.43730710933544614 1103 108-109 0.3252045574203777 1103 110-111 0.42670391612361414 1103 112-113 0.6028669850328612 1103 114-115 0.3412261935520071 1103 116-117 0.3687277434231859 1103 118-119 0.4603066335498056 1103 120-121 0.4317613028095444 1103 122-123 0.3328274208763986 1103 124-125 0.1753453942839016 1104 1 0.2534213147097546 1104 2 0.32560850831720956 1104 3 0.3682371697295146 1104 4 0.4087604330741712 1104 5 0.2592996205838176 1104 6 0.26833215492757034 1104 7 0.34046767177488135 1104 8 0.31296802536645174 1104 9 0.3878868105413673 1104 10-11 0.25044926258683375 1104 12-13 0.27529484077389554 1104 14-15 0.3522778456304607 1104 16-17 0.4076487654442076 1104 18-19 0.274684951769558 1104 20-21 0.35648554006276356 1104 22-23 0.4383342314333376 1104 24-25 0.3631899209807088 1104 26-27 0.45261896715966543 1104 28-29 0.48273919672423915 1104 30-31 0.4791259121911793 1104 32-33 0.4527576523017274 1104 34-35 0.4516164059895118 1104 36-37 0.4834806167806889 1104 38-39 0.3145519807733237 1104 40-41 0.49418220197622276 1104 42-43 0.45605077349403444 1104 44-45 0.3747018438230896 1104 46-47 0.45834797084229706 1104 48-49 0.5238614039534752 1104 50-51 0.552058467430399 1104 52-53 0.43371383319126977 1104 54-55 0.5518674195962419 1104 56-57 0.46596530678636583 1104 58-59 0.48281729375477767 1104 60-61 0.497842062965141 1104 62-63 0.43314451863360404 1104 64-65 0.37340482090032623 1104 66-67 0.40942557720614303 1104 68-69 0.499785733718511 1104 70-71 0.52763350646606 1104 72-73 0.44374838020063123 1104 74-75 0.519131573210089 1104 76-77 0.6366308810233718 1104 78-79 0.5816561768989956 1104 80-81 0.4644503117782506 1104 82-83 0.6186475357262857 1104 84-85 0.5485416471576343 1104 86-87 0.5165124706723105 1104 88-89 0.6115887390620571 1104 90-91 0.6592978592401302 1104 92-93 0.6562063988721789 1104 94-95 0.6179134266920983 1104 96-97 0.4240352361805968 1104 98-99 0.5060524874058423 1104 100-101 0.5476465915948303 1104 102-103 0.6209106890093423 1104 104-105 0.6436780393173258 1104 106-107 0.6583547704725987 1104 108-109 0.7412622411280481 1104 110-111 0.6472465028412131 1104 112-113 0.7310934109473877 1104 114-115 0.7563447545108097 1104 116-117 0.6333019025531925 1104 118-119 0.6275211218199814 1104 120-121 0.7452395556729456 1104 122-123 0.7100594815274199 1104 124-125 0.8210133288978056 1105 1 0.3295693608402708 1105 2 0.3303466370693613 1105 3 0.29869360588270766 1105 4 0.46782168538695856 1105 5 0.4355962035412233 1105 6 0.44474761793374284 1105 7 0.5183729294347401 1105 8 0.44596824162696436 1105 9 0.51822265932595 1105 10-11 0.5147472758988698 1105 12-13 0.6448952836653206 1105 14-15 0.7039386252053461 1105 16-17 0.7107459385019439 1105 18-19 0.6658965860793558 1105 20-21 0.6798187216343337 1105 22-23 0.6135487213936628 1105 24-25 0.6850906928157485 1105 26-27 0.7060860911351199 1105 28-29 0.6238417522658892 1105 30-31 0.5862524865613921 1105 32-33 0.6807455568891072 1105 34-35 0.6651924759415735 1105 36-37 0.6200440728007592 1105 38-39 0.5905718514470024 1105 40-41 0.7104458779243856 1105 42-43 0.6656075279675306 1105 44-45 0.5785235844546435 1105 46-47 0.5531439018564654 1105 48-49 0.5278500509088246 1105 50-51 0.597539096938192 1105 52-53 0.5528673693036126 1105 54-55 0.6026464317030289 1105 56-57 0.6471691696291444 1105 58-59 0.7168657095516551 1105 60-61 0.5315020318792705 1105 62-63 0.5969131679412527 1105 64-65 0.6061967757562599 1105 66-67 0.6213521864815661 1105 68-69 0.7051206656091011 1105 70-71 0.6062987427780939 1105 72-73 0.6091372444282399 1105 74-75 0.6269575163778285 1105 76-77 0.7123328108006675 1105 78-79 0.6647571250306257 1105 80-81 0.649555716647022 1105 82-83 0.7457701453204102 1105 84-85 0.5890027563084921 1105 86-87 0.7400833174903063 1105 88-89 0.8441993702265904 1105 90-91 0.7153406332934189 1105 92-93 0.6407063059180977 1105 94-95 0.6312335251348955 1105 96-97 0.6433815729677761 1105 98-99 0.7904739525897959 1105 100-101 0.8092549438035341 1105 102-103 0.6450180452814678 1105 104-105 0.7310137730158992 1105 106-107 0.6370984802636386 1105 108-109 0.5852139973278341 1105 110-111 0.5551696367722343 1105 112-113 0.7325477945227163 1105 114-115 0.6932837892806951 1105 116-117 0.6138394047821976 1105 118-119 0.6448183270006211 1105 120-121 0.5264743399752128 1105 122-123 0.5614063039522463 1105 124-125 0.5894435305006667 1106 1 -0.0484134774168723 1106 2 -0.02686471600821605 1106 3 0.02944223385291167 1106 4 -0.4729241463039813 1106 5 -0.20045748379555306 1106 6 0.05548655540275149 1106 7 0.049288047811074875 1106 8 0.0656381214114532 1106 9 0.08681036678429876 1106 10-11 0.07184374569583696 1106 12-13 0.19167339109573334 1106 14-15 0.15344021063137347 1106 16-17 0.05727921070304376 1106 18-19 0.08187161006824795 1106 20-21 0.08311309388495403 1106 22-23 0.1679181297922483 1106 24-25 0.09547193318152836 1106 26-27 0.0649974166174303 1106 28-29 0.07863804500600224 1106 30-31 -0.021310698477250867 1106 32-33 0.10405935914592135 1106 34-35 0.10433067676098062 1106 36-37 0.1888323895600834 1106 38-39 0.15803522799862435 1106 40-41 0.15472044253329642 1106 42-43 0.022552882300210797 1106 44-45 -0.07571786277453896 1106 46-47 -0.002544321274982053 1106 48-49 0.023561146563324087 1106 50-51 -0.02590805690586251 1106 52-53 -0.008708660243222255 1106 54-55 0.048859709098529436 1106 56-57 0.018043853746064542 1106 58-59 0.02179705369829321 1106 60-61 -0.04603147217104464 1106 62-63 0.03347438149582871 1106 64-65 0.04817750317477021 1106 66-67 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10-11 -0.7975100789956713 2216 12-13 -0.8472117756145252 2216 14-15 -1.0789354010424006 2216 16-17 -0.9215181380860074 2216 18-19 -1.0573827406741785 2216 20-21 -1.0795565648014716 2216 22-23 -1.1390640909411118 2216 24-25 -0.9783870533559167 2216 26-27 -1.0010205202044489 2216 28-29 -1.0872863529570935 2216 30-31 -0.9350450022284491 2216 32-33 -0.8268408166122967 2216 34-35 -0.9258074976480799 2216 36-37 -1.0665749671984983 2216 38-39 -1.1262731886630704 2216 40-41 -1.0488780118957735 2216 42-43 -0.9513908475369206 2216 44-45 -0.8853127469714437 2216 46-47 -1.1027020294735195 2216 48-49 -0.950362315246366 2216 50-51 -0.9586074981201733 2216 52-53 -0.9699458665289171 2216 54-55 -1.113858898786269 2216 56-57 -1.0040035535525647 2216 58-59 -1.2351326996130467 2216 60-61 -1.1774788081874092 2216 62-63 -1.240838073474741 2216 64-65 -1.1001098867468926 2216 66-67 -1.0973874079693573 2216 68-69 -1.3655320739988142 2216 70-71 -1.2901355231513598 2216 72-73 -1.2577617038701305 2216 74-75 -1.1355774320261816 2216 76-77 -1.135523756133253 2216 78-79 -1.377489598402768 2216 80-81 -1.2994267339332524 2216 82-83 -1.4839838603790447 2216 84-85 -1.4361261754031425 2216 86-87 -1.3530744077187862 2216 88-89 -1.3242701913253256 2216 90-91 -1.3445677538574792 2216 92-93 -1.2752002863645906 2216 94-95 -1.2068257075874271 2216 96-97 -1.2299955497642365 2216 98-99 -1.1054818199103984 2216 100-101 -1.18961251817721 2216 102-103 -1.142495787787503 2216 104-105 -1.5453406507956728 2216 106-107 -1.3179265034111367 2216 108-109 -1.347287617069643 2216 110-111 -1.4446289195654458 2216 112-113 -1.6809386256369763 2216 114-115 -1.589533661851096 2216 116-117 -1.7092691091383898 2216 118-119 -1.754055789353032 2216 120-121 -1.4681397607704376 2216 122-123 -1.255925333940084 2216 124-125 -1.0388834617590277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19944.0 3 4139.0 4 2786.0 5 1923.0 6 2268.0 7 2468.0 8 2917.0 9 3517.0 10 4329.0 11 4965.0 12 4789.0 13 4574.0 14 4657.0 15 4624.0 16 4484.0 17 4411.0 18 4244.0 19 4071.0 20 3950.0 21 4174.0 22 4992.0 23 6912.0 24 10209.0 25 15818.0 26 18973.0 27 16302.0 28 17911.0 29 22920.0 30 26164.0 31 32835.0 32 43281.0 33 58701.0 34 91535.0 35 119585.0 36 245713.0 37 351083.0 38 57027.0 39 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.81977162041335 10.592420968172874 7.147317908250235 14.440489503163548 2 11.55994595708032 31.29615422668507 42.570152802624946 14.573747013609667 3 36.65610343105137 20.12799138507048 19.66553898450221 23.550366199375937 4 14.563672885835397 15.639559070749634 46.40940117084833 23.387366872566645 5 16.7211022475354 33.51574928928322 27.939561909796947 21.823586553384438 6 31.270752098622484 37.83358428423047 15.110162984296718 15.785500632850324 7 43.585117700096646 23.619967177597736 16.484338005876896 16.310577116428714 8 40.92953486769291 25.149496677626377 15.69773900211221 18.223229452568503 9 53.60148997534275 11.65531103721776 11.107111032190636 23.636087955248847 10-11 39.17846216965902 25.365095041064322 19.315029728964923 16.14141306031173 12-13 29.102560672142992 28.745756335192556 23.254999355392687 18.896683637271764 14-15 25.148685365954513 28.744823884363125 21.872782895446317 24.23370785423604 16-17 23.870376361479565 32.36398988412474 21.65661646837897 22.109017286016723 18-19 23.783658434342083 26.234950040906213 25.116495541668794 24.86489598308291 20-21 24.135435645081646 27.599409393752904 22.575688655478878 25.68946630568657 22-23 25.163645120565892 24.441887637242573 22.726705148506984 27.66776209368455 24-25 22.707245305110074 23.78086108185378 31.3595781754613 22.15231543757485 26-27 19.785216255421485 23.099570497378146 30.419034661031862 26.696178586168507 28-29 22.746874211913795 24.356374304760994 23.855648150475712 29.0411033328495 30-31 23.062063116380404 27.34274216761574 22.29376417426614 27.301430541737716 32-33 29.02845110187309 24.703095493505682 20.57229777776697 25.69615562685426 34-35 27.672870302576243 20.31859006947975 25.847131578541955 26.161408049402056 36-37 24.837408954284772 25.863064325323176 27.610679886386947 21.688846834005105 38-39 23.05804952367979 29.159278007376905 24.579647111956533 23.20302535698677 40-41 21.33013705405539 28.44015571118025 26.10031224940384 24.129394985360523 42-43 22.06721916405378 22.945993258785915 23.86279513082285 31.123992446337457 44-45 23.645209756841147 24.056055700558414 23.115618227062317 29.183116315538115 46-47 25.055034869606885 22.359400604390302 24.054069174878315 28.531495351124498 48-49 19.39396371874364 23.14967295300691 26.07266305524406 31.38370027300539 50-51 23.509689586993034 24.667145324953164 25.23885953919883 26.58430554885497 52-53 24.765204827014152 22.170883371482734 27.956213730703332 25.107698070799778 54-55 21.789105568839602 24.013811623850955 27.622315251084682 26.57476755622476 56-57 21.36529469882263 25.852113209949067 26.013630892364926 26.768961198863376 58-59 22.85116706356639 23.55905940846941 29.865386533301873 23.72438699466233 60-61 25.275904092161817 27.96655177250794 26.973126767095675 19.784417368234564 62-63 26.63448495064903 25.541368789168 25.171469599265066 22.652676660917905 64-65 29.92044421182281 20.85688999019286 26.979276088140175 22.243389709844152 66-67 29.65196041864644 24.360054422739495 23.833381442429626 22.15460371618444 68-69 23.847556046048293 28.427167099001238 24.933690228968388 22.791586625982077 70-71 24.59125079426354 27.93358476733299 23.070481943100116 24.404682495303355 72-73 22.930079104327547 28.164294726053836 23.462711666509904 25.44291450310871 74-75 21.473391345527734 28.278958051036785 25.122183217657636 25.12546738577785 76-77 22.5576541674302 27.885748989298648 23.055666819643523 26.500930023627628 78-79 23.07033657492977 28.754029359644495 20.9284666277194 27.24716743770633 80-81 22.033760874862107 23.21308466003374 25.384671831436446 29.368482633667707 82-83 24.070650583106097 20.04505764573193 27.936672804625605 27.947618966536368 84-85 25.705478260552216 21.376403890688632 30.228422042477714 22.689695806281435 86-87 27.220722377116875 23.31980972621284 24.781445832662694 24.678022064007596 88-89 23.118636452661836 25.376292984420324 26.825100553627856 24.679970009289985 90-91 20.472258349090414 27.121286815621076 26.580059482302882 25.82639535298563 92-93 23.5574778731638 23.085787410450582 26.996483710399165 26.360251005986452 94-95 22.639377996735902 22.606412634694724 27.76210605277296 26.992103315796413 96-97 21.984155300757365 21.232180794991972 28.203361930555783 28.580301973694883 98-99 21.07862156537688 23.95654312098298 28.955170751543562 26.009664562096575 100-101 20.129701381138084 27.924514379979804 26.09571355422407 25.850070684658043 102-103 21.509694650732296 26.474306114688208 26.01404027701087 26.001958957568622 104-105 21.832661828153594 23.611751894714907 25.832624529817995 28.72296174731351 106-107 21.092362704635566 25.546343520177732 23.872000908140016 29.48929286704668 108-109 23.14732155526312 25.599991567401194 25.506259988372744 25.746426888962944 110-111 24.208004644415958 26.545050753704057 23.537126543307476 25.70981805857251 112-113 21.51163332829261 26.838124250732896 25.105480185698642 26.544762235275847 114-115 22.12704296081383 23.770412376943934 26.613721625797655 27.488823036444582 116-117 24.597566384417853 23.24660729792777 25.18922670636475 26.966599611289627 118-119 22.216325609853076 25.038300931718855 26.064433203012786 26.68094025541528 120-121 20.310068090920332 25.146084315979166 26.954152654207995 27.589694938892507 122-123 21.560474962770634 26.03825633993887 27.98403546449233 24.417233232798168 124-125 22.583555754437345 27.316603689690755 23.794038667334842 26.30580188853705 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 11.0 2 18.0 3 23.0 4 27.0 5 32.5 6 44.0 7 42.0 8 46.0 9 64.0 10 76.5 11 90.0 12 99.5 13 133.5 14 186.0 15 227.5 16 262.0 17 306.0 18 365.0 19 463.5 20 581.5 21 710.5 22 905.0 23 1156.5 24 1377.5 25 1668.0 26 2105.5 27 2561.0 28 2949.0 29 3509.0 30 4382.0 31 5209.5 32 6072.0 33 7114.0 34 8451.0 35 9342.5 36 10274.0 37 11754.5 38 12621.0 39 12926.0 40 13401.0 41 14885.0 42 16304.0 43 17804.0 44 19699.0 45 24039.0 46 40688.5 47 71057.0 48 95932.0 49 111649.5 50 112005.5 51 93887.5 52 74056.5 53 59091.0 54 50225.5 55 44719.0 56 39774.0 57 35142.5 58 31680.0 59 27472.0 60 23861.0 61 20223.0 62 15615.5 63 11994.5 64 9901.0 65 9066.0 66 7932.5 67 6705.0 68 5851.0 69 4944.0 70 3951.0 71 2808.5 72 1925.5 73 1435.0 74 1111.5 75 919.5 76 696.5 77 565.5 78 462.5 79 298.0 80 294.0 81 337.0 82 321.0 83 179.5 84 46.0 85 32.0 86 27.5 87 18.5 88 15.5 89 14.5 90 14.0 91 12.5 92 11.0 93 7.5 94 3.5 95 1.5 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15713823543005037 2 0.017432776376398777 3 0.008189350762866402 4 0.015081378632605454 5 0.003810885998561593 6 0.0 7 4.054134041022972E-4 8 3.2433072328183775E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0017838189780501074 28-29 0.008108268082045943 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.054134041022972E-5 52-53 0.0 54-55 0.0 56-57 6.081201061534457E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0019054429992807966 66-67 8.108268082045944E-5 68-69 0.006648779827277673 70-71 0.02014904618388417 72-73 8.108268082045944E-5 74-75 0.009729921698455131 76-77 5.67578765743216E-4 78-79 0.0014189469143580402 80-81 0.0014189469143580402 82-83 0.0 84-85 0.0212842037153706 86-87 0.002716269807485391 88-89 0.02112203835372968 90-91 8.108268082045944E-5 92-93 0.015486792036707752 94-95 0.015932746781220277 96-97 0.00721635859302089 98-99 0.003283848573228607 100-101 0.0011756988718966618 102-103 0.0 104-105 8.108268082045943E-4 106-107 0.001540570935588729 108-109 0.0 110-111 0.0 112-113 0.0016621949568194183 114-115 0.006527155806046984 116-117 0.0 118-119 0.07808262163010243 120-121 0.0025541044458444718 122-123 0.006121742401944687 124-125 0.2511536038413731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1233309.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.55664703764052 #Duplication Level Percentage of deduplicated Percentage of total 1 81.9744253347818 36.5250553575529 2 9.543448567899397 8.504481387235389 3 2.7967908824411603 3.738468725610658 4 1.39271093943984 2.4821811901632675 5 0.8396493283730204 1.8705979379854298 6 0.5385210868220377 1.4396816392713663 7 0.39287163946937365 1.225353007965523 8 0.29231263046092926 1.0419576560073498 9 0.2450422684146255 0.9826435676747914 >10 1.6908382261176298 14.815923111661009 >50 0.1661246855159085 5.141493981415856 >100 0.11213125032002708 9.768815425241216 >500 0.00784007081452362 2.3760753338196237 >1k 0.006199125760321003 5.288246165720337 >5k 3.646544564894707E-4 1.205422791411911 >10k+ 7.293089129789414E-4 3.5936027212633705 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11816 0.9580729565745486 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 11725 0.9506944326198868 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10683 0.8662062792049681 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 10011 0.8117187176936193 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7876 0.6386071941419385 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6962 0.5644976238720386 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4991 0.40468365997491307 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3918 0.31768194345456 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3399 0.2756000321087416 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3203 0.2597078266679315 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3094 0.2508698144585015 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2982 0.24178855420661002 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2314 0.18762532341854313 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2272 0.18421985082408382 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2211 0.1792738072940358 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2206 0.1788683938899335 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2189 0.1774899883159857 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1969 0.1596517985354846 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1969 0.1596517985354846 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1922 0.155840912536923 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1892 0.15340843211230923 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1753 0.1421379394782654 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1623 0.13159719097160566 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1541 0.12494841114432799 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1400 0.1135157531486432 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1392 0.11286709170207952 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1390 0.11270492634043862 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1366 0.11075894200074758 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1346 0.10913728838433838 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1327 0.10759671744874967 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1261 0.10224526051459935 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1235 0.10013711081326741 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.108268082045944E-5 0.0 0.0 0.0 0.0 7 8.108268082045944E-5 0.0 0.0 0.0 0.0 8 8.108268082045944E-5 0.0 0.0 0.0 0.0 9 8.108268082045944E-5 0.0 0.0 0.0 0.0 10-11 8.108268082045944E-5 0.0 0.0 0.0 0.0 12-13 8.108268082045944E-5 0.0 0.0 0.0 0.0 14-15 8.108268082045944E-5 0.0 0.0 0.0 0.0 16-17 8.108268082045944E-5 0.0 0.0 0.0 0.0 18-19 8.108268082045944E-5 0.0 0.0 0.0 0.0 20-21 8.108268082045944E-5 0.0 0.0 3.6487206369206743E-4 0.0 22-23 8.108268082045944E-5 0.0 0.0 0.001864901658870567 0.0 24-25 8.108268082045944E-5 0.0 0.0 0.003810885998561593 0.0 26-27 8.108268082045944E-5 0.0 0.0 0.0059190356998935385 0.0 28-29 1.6216536164091887E-4 0.0 0.0 0.010621831187480186 0.0 30-31 2.432480424613783E-4 0.0 0.0 0.03000059190356999 0.0 32-33 2.432480424613783E-4 0.0 0.0 0.06997435354805649 0.0 34-35 2.432480424613783E-4 0.0 0.0 0.11570498553079561 0.0 36-37 2.432480424613783E-4 0.0 0.0 0.1757061693379356 0.0 38-39 2.432480424613783E-4 0.0 0.0 0.27754601644843263 0.0 40-41 2.432480424613783E-4 0.0 0.0 0.43103553124156235 0.0 42-43 3.2433072328183775E-4 0.0 0.0 0.7837451928105608 0.0 44-45 4.054134041022972E-4 0.0 0.0 1.2694709922655232 0.0 46-47 4.054134041022972E-4 0.0 0.0 1.741007322576905 0.0 48-49 4.054134041022972E-4 0.0 0.0 1.9399842213103122 0.0 50-51 4.054134041022972E-4 0.0 0.0 2.1932865161934276 0.0 52-53 4.054134041022972E-4 0.0 0.0 2.399844645583548 0.0 54-55 4.054134041022972E-4 0.0 0.0 2.6148759151194065 0.0 56-57 4.054134041022972E-4 0.0 0.0 2.926436116172022 0.0 58-59 4.054134041022972E-4 0.0 0.0 3.2770376280396887 0.0 60-61 4.054134041022972E-4 0.0 0.0 3.7848179166778158 0.0 62-63 4.054134041022972E-4 0.0 0.0 4.4330739498373894 0.0 64-65 4.054134041022972E-4 0.0 0.0 5.0392886129915535 0.0 66-67 4.054134041022972E-4 0.0 0.0 5.536649777144252 0.0 68-69 4.054134041022972E-4 0.0 0.0 5.937968505865116 0.0 70-71 4.054134041022972E-4 0.0 0.0 6.412950850111367 0.0 72-73 4.054134041022972E-4 0.0 0.0 6.860446165559482 1.6216536164091887E-4 74-75 4.054134041022972E-4 0.0 0.0 7.334901472380401 1.6216536164091887E-4 76-77 4.054134041022972E-4 0.0 0.0 7.899723426975721 1.6216536164091887E-4 78-79 4.054134041022972E-4 0.0 0.0 8.606642779708897 1.6216536164091887E-4 80-81 4.864960849227566E-4 0.0 0.0 10.127429541177435 1.6216536164091887E-4 82-83 4.864960849227566E-4 0.0 0.0 11.220626785339277 1.6216536164091887E-4 84-85 5.67578765743216E-4 0.0 0.0 11.763272626730203 1.6216536164091887E-4 86-87 6.486614465636755E-4 0.0 0.0 12.25536341662957 1.6216536164091887E-4 88-89 6.486614465636755E-4 0.0 0.0 12.808063510442233 1.6216536164091887E-4 90-91 6.486614465636755E-4 0.0 0.0 13.461954789918828 1.6216536164091887E-4 92-93 6.486614465636755E-4 0.0 0.0 14.303795723537249 1.6216536164091887E-4 94-95 6.486614465636755E-4 0.0 0.0 15.436480233258656 1.6216536164091887E-4 96-97 6.486614465636755E-4 0.0 0.0 16.73485720123667 1.6216536164091887E-4 98-99 6.486614465636755E-4 0.0 0.0 18.012193213541778 1.6216536164091887E-4 100-101 6.486614465636755E-4 0.0 4.054134041022972E-5 19.099755211386604 1.6216536164091887E-4 102-103 7.297441273841349E-4 0.0 8.108268082045944E-5 20.069382449978065 1.6216536164091887E-4 104-105 7.297441273841349E-4 0.0 8.108268082045944E-5 21.122038353729682 1.6216536164091887E-4 106-107 7.297441273841349E-4 0.0 8.108268082045944E-5 22.112220051909134 1.6216536164091887E-4 108-109 7.297441273841349E-4 0.0 8.108268082045944E-5 22.947250040338634 1.6216536164091887E-4 110-111 7.297441273841349E-4 0.0 8.108268082045944E-5 23.884646913303968 1.6216536164091887E-4 112-113 7.297441273841349E-4 0.0 8.108268082045944E-5 26.409683218074303 1.6216536164091887E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTA 1620 0.0 92.20232 2 GAGTAGT 2145 0.0 89.45269 1 GAGTCGG 730 0.0 79.99596 1 GAGTAAG 1170 0.0 77.92385 1 GAGTCAG 4805 0.0 77.6329 1 GAGTTAG 1160 0.0 77.568214 1 GAGTCGT 690 0.0 75.99734 1 AGTCCGT 285 0.0 75.16934 2 GAGTACG 620 0.0 74.96658 1 GAGTCCG 675 0.0 71.50659 1 TAAGCGG 175 0.0 71.39929 4 GAGTAAC 1020 0.0 70.1045 1 AGTAGCG 170 0.0 70.010666 2 AGTACGG 335 0.0 69.27921 2 TCGTAGG 955 0.0 69.1676 2 GAGTCGA 365 0.0 68.56796 1 AGTCGTA 460 0.0 68.56479 2 GAGTAGG 1455 0.0 67.57496 1 GTAAGCG 185 0.0 67.53987 3 GAGTAGC 810 0.0 66.20981 1 >>END_MODULE