FastQCFastQC Report
Thu 26 May 2016
SRR935950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291085
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT36830.16075352944129093No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT32920.14368737956033933No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC32650.14250889862226848No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT32320.14106853303129302No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT32100.14010828930397606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.051.0726851
GTATCAA93400.047.3998951
GAGTAAG17750.043.9752581
GAGTAGT14900.038.39021
TATCAAC114900.038.3688742
AGGGTAA23000.038.2779276
GATTGTG32150.035.525069
GTAAGGG15850.035.2825933
GAGTAGG25850.035.2668231
GGGGTAA35350.035.001649
ATCAACG125900.034.8260083
TAAGGGG17350.034.6324964
TCAACGC127100.034.5440064
AGTAAGG15000.033.7138672
CAACGCA131150.033.6096845
GAGTTAG12000.033.2681771
AACGCAG133600.032.6816646
GATAGCG6300.032.103549
GTAGGGG20200.031.8078483
GTATACG2550.030.3765621
Produced by FastQC (version 0.11.5)