FastQCFastQC Report
Thu 26 May 2016
SRR935950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291085
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT37150.16215024759011562No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT35790.1562141954576107No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT34970.15263510520124743No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT31460.1373148530063267No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC26970.11771715148063036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.053.2739831
GTATCAA100850.046.226941
GATTGTG30800.040.5653349
AGGGTAA25450.038.8067056
CGGGATT9200.038.1549266
TATCAAC124800.036.9003032
GGGATTG36600.035.274847
TAAGCGG2051.8189894E-1234.873254
ATCAACG135350.033.8480073
TCAACGC137900.033.26534
GTCTAAC19350.033.0082741
GAGTACG8450.032.4953421
GAGTAGT15450.032.4541931
CAACGCA142350.032.274545
AACGCAG144700.031.7831866
GAGTAAG16000.031.7116581
TATACGG2450.031.6112962
GTATAGG8400.031.2675191
TATCGGG3850.030.948122
GAGTCCG7850.030.4165781
Produced by FastQC (version 0.11.5)