FastQCFastQC Report
Thu 26 May 2016
SRR935949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437875
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT9360.2137596345989152No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8650.19754496146160436No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7730.1765343990864973No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7290.16648586925492437No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6150.14045104196403083No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5460.12469312018270054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGG551.9917934E-975.7097553
TATAAGG701.7462298E-1067.9842762
GTATAAG805.638867E-1059.6019481
CTTAAGG401.7727909E-459.4862373
TCTTAAG401.7727909E-459.4862372
GCGGTCT350.007800176350.988219
GGAAACA1300.050.4324151
ACAAAGT558.5323316E-443.262729
TTCCATA558.5323316E-443.262722
GTCTAGG1104.6286732E-737.928511
ATACTGG1104.699723E-737.8548776
CTTGGGC401.198868E-437.17889816-17
TACTGGT1156.65701E-736.2090157
TACTAGG1652.3646862E-1036.0522652
CACCGAT851.7110826E-434.9919058
TTAATGG1209.287778E-734.700314
GTACTGG1555.0204108E-934.607581
TAAGGGC1051.4839432E-533.9921384
TATACTG1051.4839432E-533.9921385
CACAAAG1408.0626705E-833.9921389