##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437875 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74279646017699 31.0 31.0 34.0 25.0 34.0 2 29.774707393662574 31.0 31.0 34.0 25.0 34.0 3 29.761975449614617 33.0 31.0 34.0 23.0 34.0 4 33.026473308592635 37.0 35.0 37.0 26.0 37.0 5 33.15053611190408 37.0 35.0 37.0 26.0 37.0 6 33.28622323722524 37.0 35.0 37.0 27.0 37.0 7 33.2729203539823 37.0 35.0 37.0 27.0 37.0 8 33.17508192977448 37.0 35.0 37.0 26.0 37.0 9 34.5684658863831 39.0 37.0 39.0 25.0 39.0 10-11 34.50369511846988 39.0 35.0 39.0 25.0 39.0 12-13 34.420577790465316 39.0 35.0 39.0 24.0 39.0 14-15 35.4205789323437 40.0 36.0 41.0 23.0 41.0 16-17 35.37964487582072 40.0 36.0 41.0 24.5 41.0 18-19 35.452953468455604 40.0 36.0 41.0 24.5 41.0 20-21 35.547545532400804 40.0 36.0 41.0 25.0 41.0 22-23 35.29349586069084 40.0 35.5 41.0 23.5 41.0 24-25 35.052306023408505 40.0 35.0 41.0 23.0 41.0 26-27 34.92088267199543 40.0 35.0 41.0 22.0 41.0 28-29 34.79511276049101 40.0 35.0 41.0 20.5 41.0 30-31 34.655907507850415 40.0 35.0 41.0 18.0 41.0 32-33 34.41234941478733 39.0 35.0 41.0 15.0 41.0 34-35 34.27155580930631 39.0 35.0 41.0 11.5 41.0 36-37 34.19217470739366 39.0 35.0 41.0 8.0 41.0 38-39 34.098634313445615 39.0 35.0 41.0 4.5 41.0 40-41 33.8819777333714 39.0 34.0 41.0 2.0 41.0 42-43 33.64711504424778 39.0 34.0 41.0 2.0 41.0 44-45 33.33981159006566 38.0 34.0 41.0 2.0 41.0 46-47 33.028497858978014 37.5 33.0 40.0 2.0 41.0 48-49 32.66053554096489 37.0 33.0 40.0 2.0 41.0 50-51 32.280728518412786 36.0 33.0 39.0 2.0 41.0 52-53 31.908636026263203 35.5 33.0 39.0 2.0 41.0 54-55 31.496846131886954 35.0 32.5 38.5 2.0 41.0 56-57 31.141591778475593 35.0 32.0 37.0 2.0 40.0 58-59 30.785767627747646 35.0 32.0 37.0 2.0 39.5 60-61 30.456382529260637 35.0 31.5 36.0 2.0 39.0 62-63 30.12333656865544 35.0 31.0 35.5 2.0 38.5 64-65 29.88306594347702 35.0 31.0 35.0 2.0 37.0 66-67 29.717259491864116 35.0 31.0 35.0 2.0 37.0 68-69 29.58945132743363 35.0 31.0 35.0 2.0 36.0 70-71 29.349962888952327 35.0 31.0 35.0 2.0 36.0 72-73 29.129329146445905 35.0 31.0 35.0 2.0 35.0 74-75 28.929270910648015 35.0 30.0 35.0 2.0 35.0 76-77 28.76023408506994 35.0 30.0 35.0 2.0 35.0 78-79 28.61199885812161 35.0 30.0 35.0 2.0 35.0 80-81 28.411915500999143 34.5 29.0 35.0 2.0 35.0 82-83 28.20682843277191 34.0 29.0 35.0 2.0 35.0 84-85 27.99594747359406 34.0 29.0 35.0 2.0 35.0 86-87 27.823145874964318 34.0 29.0 35.0 2.0 35.0 88-89 27.564197544961463 34.0 28.0 35.0 2.0 35.0 90-91 27.250998572652012 34.0 26.0 35.0 2.0 35.0 92-93 26.899632315158435 34.0 25.0 35.0 2.0 35.0 94-95 26.569280045675136 34.0 24.0 35.0 2.0 35.0 96-97 26.257268626891236 33.5 23.5 35.0 2.0 35.0 98-99 25.89442763345704 33.0 20.0 35.0 2.0 35.0 100-101 25.60982015415358 33.0 19.5 35.0 2.0 35.0 102-103 24.827446188980872 31.5 19.0 34.5 2.0 35.0 104-105 25.24943077362261 33.0 19.0 35.0 2.0 35.0 106-107 25.275381101912647 33.0 19.0 35.0 2.0 35.0 108-109 25.080344847273764 33.0 17.5 35.0 2.0 35.0 110-111 24.744368826719956 33.0 12.5 35.0 2.0 35.0 112-113 24.387354838709676 32.5 4.5 35.0 2.0 35.0 114-115 23.87932857550671 32.0 2.0 35.0 2.0 35.0 116-117 23.280592634884385 32.0 2.0 35.0 2.0 35.0 118-119 22.478097630602342 31.0 2.0 35.0 2.0 35.0 120-121 21.538880959177845 31.0 2.0 35.0 2.0 35.0 122-123 20.553259491864118 30.0 2.0 35.0 2.0 35.0 124-125 17.79786811304596 23.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40395370197908775 1101 2 0.5234885437530217 1101 3 0.4483165478293145 1101 4 0.42275590900334237 1101 5 0.5457121071516298 1101 6 0.2109753560201426 1101 7 0.19453658548170694 1101 8 0.12399406215228481 1101 9 0.1731373759186141 1101 10-11 0.4449870572281043 1101 12-13 0.09748988422993676 1101 14-15 0.19547016970941655 1101 16-17 0.01843812321839522 1101 18-19 0.03520810317053957 1101 20-21 0.17304450405430316 1101 22-23 0.2505264442180106 1101 24-25 0.26862465461600493 1101 26-27 0.28183355933801124 1101 28-29 0.1538494356789073 1101 30-31 0.07843991258507543 1101 32-33 0.16297126459456024 1101 34-35 -0.21628831100975532 1101 36-37 0.011687355457084436 1101 38-39 0.2901018766104144 1101 40-41 0.12708858455223293 1101 42-43 -0.035204027232900614 1101 44-45 0.0071775691417030885 1101 46-47 0.0983594511981849 1101 48-49 0.06941622386217006 1101 50-51 0.24487307013431092 1101 52-53 0.3577906057994511 1101 54-55 -0.17342756225831835 1101 56-57 -0.03235121066406066 1101 58-59 -0.18804767824462232 1101 60-61 -0.07044929396921873 1101 62-63 -0.022726417292378898 1101 64-65 -0.13162828757695166 1101 66-67 -0.13138289771534417 1101 68-69 -0.03280883164161352 1101 70-71 0.12067162037552492 1101 72-73 0.18746178460119367 1101 74-75 0.1516495422665436 1101 76-77 0.276688509020957 1101 78-79 0.362903421047271 1101 80-81 0.2680963835429502 1101 82-83 0.1866633050854425 1101 84-85 0.29481886370725974 1101 86-87 0.20447472685203394 1101 88-89 0.12555418604103608 1101 90-91 -0.15301135037402247 1101 92-93 -0.6742831030558918 1101 94-95 -0.6310465220739836 1101 96-97 -0.40083465746571534 1101 98-99 -0.6436706523719629 1101 100-101 -0.30082189800678094 1101 102-103 -0.23059380120572115 1101 104-105 -0.350073225187991 1101 106-107 0.0931274218139464 1101 108-109 -0.126679597645861 1101 110-111 -0.31795006048222874 1101 112-113 -0.31550210798742384 1101 114-115 -0.4869010824366242 1101 116-117 -0.8673800548133848 1101 118-119 -0.9629302801766428 1101 120-121 -1.1251139688471916 1101 122-123 -0.6259536483013548 1101 124-125 -0.36484169047979265 1102 1 0.3107797659211897 1102 2 0.3318502479115999 1102 3 0.4601382874502207 1102 4 0.31794824728964244 1102 5 0.3354430736301879 1102 6 0.474463911754718 1102 7 0.5021881753567214 1102 8 0.505509107608276 1102 9 0.4255996357307765 1102 10-11 0.5450408581473454 1102 12-13 0.6018648245716633 1102 14-15 0.5634959363002068 1102 16-17 0.7528307947232946 1102 18-19 0.8962730142688642 1102 20-21 0.9461478047117708 1102 22-23 0.7630915621316987 1102 24-25 0.9383925875326184 1102 26-27 0.8751535677841744 1102 28-29 0.9121054181386583 1102 30-31 0.9267777245074811 1102 32-33 0.8391880765140698 1102 34-35 0.9689676338471997 1102 36-37 1.0586908467317926 1102 38-39 0.8008085487921548 1102 40-41 0.7093721732470968 1102 42-43 0.8193744020695064 1102 44-45 0.9419511858651148 1102 46-47 1.0250311122871025 1102 48-49 0.8623361964410563 1102 50-51 0.7671035521153442 1102 52-53 0.964634603381306 1102 54-55 0.950602357994832 1102 56-57 0.8378670880120289 1102 58-59 0.7679570539222524 1102 60-61 0.7693107423608652 1102 62-63 0.6398695059863506 1102 64-65 0.7722719898159625 1102 66-67 0.6564071861388818 1102 68-69 0.7875134532197947 1102 70-71 0.7621608066800079 1102 72-73 0.8864615503068656 1102 74-75 1.123771410026201 1102 76-77 1.1692009251739606 1102 78-79 1.0941880770829542 1102 80-81 1.2210364880787168 1102 82-83 1.1240532084462629 1102 84-85 1.14418785991516 1102 86-87 1.4422342945934652 1102 88-89 1.4544325525757067 1102 90-91 1.6141044286256871 1102 92-93 1.3249778574351403 1102 94-95 1.2835242709689219 1102 96-97 1.4111976577961016 1102 98-99 1.0771705510331167 1102 100-101 1.3308833308062162 1102 102-103 1.2189548206519127 1102 104-105 0.9823441426743464 1102 106-107 0.9776579220178725 1102 108-109 0.5957496006336669 1102 110-111 0.9239732934555072 1102 112-113 0.7731942031785799 1102 114-115 0.6734806148377501 1102 116-117 0.9258899129928402 1102 118-119 1.0120060120144103 1102 120-121 0.8424275894704003 1102 122-123 0.9251094983582142 1102 124-125 1.205614373549082 1103 1 -0.3991696438595831 1103 2 -0.330881623926512 1103 3 -0.18910285926141412 1103 4 -0.17446321982671975 1103 5 -0.27838491962445033 1103 6 -0.3332932551930057 1103 7 -0.28196022261966647 1103 8 -0.34609291600049374 1103 9 -0.5760867049100327 1103 10-11 -0.3352289563551807 1103 12-13 -0.33068154979596187 1103 14-15 -0.3698936083878266 1103 16-17 -0.2505418865583309 1103 18-19 -0.25128179180196497 1103 20-21 -0.49904612446191265 1103 22-23 -0.5674312034669597 1103 24-25 -0.6315905490609737 1103 26-27 -0.5658245097874897 1103 28-29 -0.6182487133959143 1103 30-31 -0.44758989775221636 1103 32-33 -0.4356854479378711 1103 34-35 -0.3674573239942802 1103 36-37 -0.4556430487150891 1103 38-39 -0.586206628273608 1103 40-41 -0.39349460584227813 1103 42-43 -0.35769136521548006 1103 44-45 -0.5007132323473655 1103 46-47 -0.39571087759485124 1103 48-49 -0.4184395202537132 1103 50-51 -0.5743804276485669 1103 52-53 -0.34986792612965445 1103 54-55 -0.3698023637589607 1103 56-57 -0.43953594740112223 1103 58-59 -0.3989906695178824 1103 60-61 -0.39848015139967785 1103 62-63 -0.5237055361721623 1103 64-65 -0.35588989888555744 1103 66-67 -0.19266532650867063 1103 68-69 -0.06830442199099807 1103 70-71 -0.08016634340430784 1103 72-73 -0.03849629117711473 1103 74-75 0.06896533919989523 1103 76-77 -0.008708012431437595 1103 78-79 0.018302748246529887 1103 80-81 -0.09683872271386562 1103 82-83 -0.06144426204277664 1103 84-85 -0.18296222440187293 1103 86-87 0.2651685273084752 1103 88-89 0.31530944970892705 1103 90-91 0.14951758208268373 1103 92-93 0.33088004967797247 1103 94-95 0.5491229218964087 1103 96-97 0.4432381299714798 1103 98-99 0.2921789827363206 1103 100-101 0.631051964870295 1103 102-103 0.6624624091847977 1103 104-105 0.5396797244618696 1103 106-107 0.5096983941932507 1103 108-109 0.510589398272792 1103 110-111 0.6195888077894054 1103 112-113 0.6188940345445175 1103 114-115 0.4087251342306679 1103 116-117 0.10548519123904754 1103 118-119 0.31470415703970644 1103 120-121 0.44866704984139716 1103 122-123 0.7623776265201023 1103 124-125 0.2595772740549869 1104 1 0.3139392937458112 1104 2 0.2737376003567924 1104 3 0.40294570734904056 1104 4 0.3708615693807005 1104 5 0.5615584193300194 1104 6 0.489446711080177 1104 7 0.4198758650700469 1104 8 0.3966362576925917 1104 9 0.5465493827796806 1104 10-11 0.3729774517392528 1104 12-13 0.5109238464940944 1104 14-15 0.5941981285430344 1104 16-17 0.4259235434585378 1104 18-19 0.3911573313009029 1104 20-21 0.5512702330422954 1104 22-23 0.5572379364993125 1104 24-25 0.5334630765714081 1104 26-27 0.7673100601956406 1104 28-29 0.8068291955416953 1104 30-31 0.7100721595833619 1104 32-33 0.5354282114213191 1104 34-35 0.9201892190074048 1104 36-37 0.7989943880471344 1104 38-39 0.6390075369877763 1104 40-41 0.8137053941577221 1104 42-43 0.7700968304000284 1104 44-45 0.6752530408398201 1104 46-47 0.74998895377108 1104 48-49 0.9651705977901273 1104 50-51 0.918740651609447 1104 52-53 0.8248396624032281 1104 54-55 0.9475467087536842 1104 56-57 0.9355939008282199 1104 58-59 0.8038329392510875 1104 60-61 0.8345208604047087 1104 62-63 0.8516118331364346 1104 64-65 0.8316581618227055 1104 66-67 0.6653690748404024 1104 68-69 0.8323026606396944 1104 70-71 0.8585803682314435 1104 72-73 0.7113478909476747 1104 74-75 0.7040763004140622 1104 76-77 1.0422747868940263 1104 78-79 0.9632835239632236 1104 80-81 0.9850445825137903 1104 82-83 0.8278845743821819 1104 84-85 1.1560003388359057 1104 86-87 0.9676170939189284 1104 88-89 1.0260413215470408 1104 90-91 0.9765543443086528 1104 92-93 1.1062918540624622 1104 94-95 1.1634015053702704 1104 96-97 0.9150816375600108 1104 98-99 1.1834127095491347 1104 100-101 1.402898170603855 1104 102-103 1.266439474952083 1104 104-105 1.567076689048715 1104 106-107 1.6625860839065751 1104 108-109 1.548971860330127 1104 110-111 1.523704828025494 1104 112-113 1.4057891441566603 1104 114-115 1.6005335659338726 1104 116-117 1.3099290360957063 1104 118-119 1.3539918467530292 1104 120-121 1.0649004393860828 1104 122-123 1.2168909486111659 1104 124-125 0.7804029064327267 1105 1 -0.15162064365637917 1105 2 -0.09434002092645954 1105 3 -0.2623913818528365 1105 4 -0.21769054329535464 1105 5 -0.16393015656982612 1105 6 -0.023151723974592642 1105 7 -0.31023743696438544 1105 8 -0.2491903463762668 1105 9 -0.29450476432517547 1105 10-11 -0.2558601069461943 1105 12-13 -0.14816222549338676 1105 14-15 -0.097268538123636 1105 16-17 -0.0158792734428701 1105 18-19 -0.19855659671161874 1105 20-21 -0.07105339763063512 1105 22-23 -0.03753890231132573 1105 24-25 0.07670814699394413 1105 26-27 0.21124996629892934 1105 28-29 0.1652993050878564 1105 30-31 0.17681269166474323 1105 32-33 0.11974871220379413 1105 34-35 -0.040391688660825764 1105 36-37 0.09633398116137926 1105 38-39 0.08549267000811511 1105 40-41 0.13389193562876756 1105 42-43 0.08699323102600687 1105 44-45 0.18698387026078933 1105 46-47 0.08066188021865628 1105 48-49 -0.17842627231942032 1105 50-51 -0.06854948923562887 1105 52-53 -0.0863290386922344 1105 54-55 -0.10147363553426203 1105 56-57 0.07190688987360616 1105 58-59 -0.056747968417145245 1105 60-61 -0.09161497684098663 1105 62-63 0.1102133980816653 1105 64-65 -0.1338629649378582 1105 66-67 -0.22716801436147804 1105 68-69 -0.10178184640256305 1105 70-71 -0.14809631408467538 1105 72-73 0.0782887360180986 1105 74-75 -0.07132025670643927 1105 76-77 0.07505350363894081 1105 78-79 0.15813047232003186 1105 80-81 0.15326617875792437 1105 82-83 0.1595653255558922 1105 84-85 0.19072584744310106 1105 86-87 0.10972038030390507 1105 88-89 0.360670429528259 1105 90-91 0.2658275837452706 1105 92-93 0.29229873982145804 1105 94-95 0.19556801554241332 1105 96-97 0.31997428231744607 1105 98-99 0.26488876588716437 1105 100-101 0.1604718795240423 1105 102-103 0.1526003513214107 1105 104-105 0.06431111784370813 1105 106-107 0.19179547357793325 1105 108-109 0.18794150039272495 1105 110-111 0.27966977325240805 1105 112-113 0.36249900331158713 1105 114-115 0.6706819227413838 1105 116-117 0.5521675337482925 1105 118-119 0.21432862296585853 1105 120-121 0.06974050198389392 1105 122-123 -0.2051860466939992 1105 124-125 -0.1412571874639852 1106 1 0.09952242627593222 1106 2 0.09518097385076985 1106 3 0.15597221337313627 1106 4 -0.8703504788120711 1106 5 -0.2497476047663696 1106 6 -0.032225578076449324 1106 7 0.2548421301302852 1106 8 0.0782579444395779 1106 9 0.13390841892425698 1106 10-11 0.13736419872720518 1106 12-13 7.491476989116563E-4 1106 14-15 0.19213909239845606 1106 16-17 0.23840547116937927 1106 18-19 0.2572007047948759 1106 20-21 0.5614437270182009 1106 22-23 0.5171740810776342 1106 24-25 0.40498958259550477 1106 26-27 0.49417391561732416 1106 28-29 0.5072846172284358 1106 30-31 0.48773360536649335 1106 32-33 0.5144354403369817 1106 34-35 0.32330488165801086 1106 36-37 0.46691607479412767 1106 38-39 0.600911151445608 1106 40-41 0.5598186471697701 1106 42-43 0.4949112882313571 1106 44-45 0.21380123361090853 1106 46-47 0.3332720863012071 1106 48-49 0.38550433272988727 1106 50-51 0.12955631426004288 1106 52-53 0.23294689131888902 1106 54-55 0.2928418894765805 1106 56-57 -0.03126754495491113 1106 58-59 0.3732136621426534 1106 60-61 0.3646702579950656 1106 62-63 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2216 108-109 -4.074602823214178 2216 110-111 -4.261452133999825 2216 112-113 -4.540146298881314 2216 114-115 -4.43164618090157 2216 116-117 -4.475838052511886 2216 118-119 -4.399366381095071 2216 120-121 -4.018886902386068 2216 122-123 -4.112782469110353 2216 124-125 -3.4545186125546152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25389.0 3 2521.0 4 1717.0 5 2356.0 6 4537.0 7 3784.0 8 2731.0 9 2610.0 10 2122.0 11 1784.0 12 1581.0 13 1574.0 14 1574.0 15 1710.0 16 1791.0 17 2037.0 18 2245.0 19 2703.0 20 2968.0 21 3534.0 22 4012.0 23 4614.0 24 5174.0 25 5560.0 26 5997.0 27 6726.0 28 7749.0 29 9386.0 30 12378.0 31 16529.0 32 21896.0 33 29485.0 34 42833.0 35 65728.0 36 99621.0 37 26674.0 38 2186.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.97026532479414 18.244510521500455 11.55352241537054 14.23170173833486 2 19.56912520813966 23.06153167948434 34.90327929886914 22.466063813506864 3 31.111522219253107 22.996704283061657 24.404978062812756 21.486795434872477 4 16.54130873226697 21.204715255143178 29.249245682517422 33.00473033007243 5 16.614938394253937 33.51352585842668 25.274228358055566 24.597307389263815 6 32.446702826149014 35.17556380245504 9.650699400513846 22.7270339708821 7 29.249510133051977 40.26363748475589 11.525461662487382 18.961390719704756 8 36.53448252236945 27.128123615469292 10.869108464992213 25.468285397169048 9 37.27547816157579 15.82026834142164 15.155466742791893 31.748786754210677 10-11 30.753525549528977 26.773051669997145 15.114016557236653 27.359406223237226 12-13 32.913731087639164 28.168084499000855 17.414901512988866 21.503282900371108 14-15 36.19708821010562 21.47142449329146 21.032372252355124 21.299115044247788 16-17 30.593091635740794 22.776591493005995 21.987896089066513 24.642420782186697 18-19 29.856808449900086 23.372537824721668 21.673879531829858 25.096774193548388 20-21 38.79646017699115 21.309962888952327 17.53833856694262 22.355238367113902 22-23 33.817071081929775 24.733314302026834 17.0682272337996 24.38138738224379 24-25 27.61815586640023 26.19434770196974 18.433799600342564 27.753696831287467 26-27 25.20899682665111 24.84963990083713 21.00645061097529 28.934912661536476 28-29 27.316600222230093 23.15292650533478 23.029135438162136 26.501337834273 30-31 29.14165001427348 24.21581501570083 20.365857836140453 26.27667713388524 32-33 25.61861261775621 23.86994005138453 22.46988295746503 28.041564373394234 34-35 24.315386811304595 24.627233799600344 22.63419925777905 28.423180131316016 36-37 25.700371110476734 25.76637168141593 20.033571224664573 28.499685983442763 38-39 26.734912931772765 22.360605195546672 21.371395946331713 29.533085926348846 40-41 24.805595204110762 22.253382814730234 23.345932058235796 29.595089922923208 42-43 24.974479017984585 22.667199543248646 24.710019982871824 27.64830145589495 44-45 25.097573508421355 21.77676277476449 25.949072223808162 27.176591493005997 46-47 23.05429631744219 22.436083357122467 25.166657151013418 29.342963174421925 48-49 21.872566371681415 22.850927776191835 26.467941764202113 28.808564087924637 50-51 23.043220097059663 23.173508421353127 25.29957179560377 28.483699685983442 52-53 22.54193548387097 23.50373965172709 24.339023693976593 29.61530117042535 54-55 20.72806166143306 22.83368541250357 24.644932914644592 31.793320011418785 56-57 21.19744629442065 22.17661036806292 25.818986533782017 30.806956803734415 58-59 22.9224093634028 20.934513274336283 25.37676277476449 30.766314587496428 60-61 22.81484441906937 21.10522409363403 24.729203539823008 31.350727947473594 62-63 22.08324293462746 20.731258920924923 25.11721381672852 32.0682843277191 64-65 21.54084549180281 20.56405706344557 25.187073140373656 32.708024304377965 66-67 22.240137025406796 21.20822152440765 24.10402512132458 32.44761632886098 68-69 21.913216859641167 20.9654929842853 24.49590431174965 32.62538584432388 70-71 21.25969503538809 20.579572099312795 25.69117279234151 32.469560072957606 72-73 21.56117613474165 19.82746217527833 26.880388238652586 31.730973451327433 74-75 21.521424582568667 19.05754905196465 27.341652458222605 32.07937390724408 76-77 20.903754166443047 19.362441435711457 27.297267361463984 32.436537036381516 78-79 21.24642301692972 19.182906303026705 28.405413491249664 31.16525718879391 80-81 22.551176894432917 19.979856966189622 28.08137602956139 29.387590109816074 82-83 21.805538110191264 20.144333428489865 28.24721667142449 29.802911789894377 84-85 21.41680752889579 20.05756315957787 28.60706290831011 29.91856640321623 86-87 21.790188517223935 19.64797328854018 28.562146508794388 29.999691685441498 88-89 22.416217148430672 18.903864581465495 28.48063026587343 30.1992880042304 90-91 23.408621181844133 18.42238081644305 28.256465886383097 29.912532115329714 92-93 24.395341347482578 19.002350339988443 27.851643068095903 28.750665244433076 94-95 23.819612106870473 19.20473000290086 27.642239282230967 29.333418607997697 96-97 23.747890215854614 19.288508842250955 26.975431720647446 29.98816922124698 98-99 23.4033976652328 19.627643113829894 26.864896159612844 30.104063061324464 100-101 23.150619077145134 20.29611562349056 26.983610413604968 29.569654885759338 102-103 23.128860976306022 21.34376248929489 27.31715672280902 28.210219811590065 104-105 22.13809058354953 22.888538202945153 27.43758449957979 27.535786713925532 106-107 21.347896338504228 24.215544656774025 26.938628694753554 27.4979303099682 108-109 21.029289180702254 25.407136739937197 26.97527833285755 26.588295746503 110-111 20.648815301170426 27.632657721952615 26.038938053097343 25.67958892377962 112-113 20.305115131860667 29.65623162257989 25.135686023740057 24.902967221819384 114-115 19.81358636133687 30.729016189574587 25.212433209582873 24.244964239505666 116-117 19.445503853839565 31.57910362546389 24.88655438195832 24.088838138738225 118-119 19.246041948772447 32.52086868582986 24.64681784679728 23.586271518600412 120-121 19.514367540457954 32.884904376264664 24.029831316716226 23.57089676656115 122-123 19.802853057924473 33.20551061332067 23.762464428132265 23.229171900622596 124-125 19.938941399082175 34.877808392295215 22.84615041203681 22.337099796585804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 1.5 6 1.5 7 1.0 8 1.5 9 1.5 10 2.5 11 5.0 12 5.5 13 6.5 14 8.0 15 9.5 16 14.5 17 27.0 18 39.5 19 46.5 20 74.0 21 110.0 22 138.5 23 204.5 24 276.0 25 355.5 26 495.0 27 616.0 28 704.5 29 786.5 30 869.5 31 943.0 32 998.0 33 1043.5 34 1086.0 35 1187.5 36 1238.5 37 1201.0 38 1182.5 39 1185.5 40 1219.0 41 1249.0 42 1309.0 43 1567.0 44 1760.5 45 1999.0 46 2802.5 47 4104.5 48 6054.0 49 9910.5 50 16890.5 51 27094.5 52 38511.0 53 46508.5 54 48251.0 55 44837.5 56 38311.0 57 30195.0 58 22692.0 59 16714.5 60 11990.0 61 8798.5 62 6878.5 63 5655.0 64 4708.5 65 4005.5 66 3487.5 67 3108.0 68 2872.5 69 2535.5 70 2036.5 71 1454.0 72 957.0 73 670.0 74 480.5 75 343.0 76 214.0 77 156.0 78 160.0 79 136.0 80 90.0 81 66.5 82 50.0 83 39.0 84 30.5 85 23.0 86 16.0 87 15.0 88 12.5 89 8.5 90 5.5 91 5.0 92 5.5 93 5.0 94 3.5 95 0.5 96 1.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15415358264344847 2 0.015529546103339995 3 0.007764773051669997 4 0.01415929203539823 5 0.0022837567799029405 6 0.0 7 2.2837567799029401E-4 8 2.2837567799029401E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0021695689409077933 28-29 0.009249214958606908 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.4256351698544105E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0026263202968883816 66-67 0.0 68-69 0.008335712246645732 70-71 0.019754496146160434 72-73 0.0 74-75 0.01107622038252926 76-77 3.4256351698544105E-4 78-79 0.0013702540679417642 80-81 0.0014844419069369112 82-83 0.0 84-85 0.021695689409077932 86-87 0.0023979446188980872 88-89 0.020896374536111904 90-91 0.0 92-93 0.015072794747359407 94-95 0.01689980017128176 96-97 0.007764773051669997 98-99 0.0035398230088495575 100-101 0.0012560662289466172 102-103 0.0 104-105 6.851270339708821E-4 106-107 0.0017128175849272054 108-109 0.0 110-111 0.0 112-113 0.0017128175849272054 114-115 0.007193833856694262 116-117 0.0 118-119 0.07250927776191836 120-121 0.0029688838138738224 122-123 0.006622894661718528 124-125 0.246988295746503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 437875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.864413814401225 #Duplication Level Percentage of deduplicated Percentage of total 1 84.29454423120548 53.834216550780546 2 7.966452097258136 10.175455867437963 3 2.685816064632748 5.145842057432172 4 1.3862689255229708 3.5413300927057745 5 0.8304697534794603 2.651873199827801 6 0.5660013452761518 2.1688406480534366 7 0.41074684533642847 1.8362474552467867 8 0.3001638168589301 1.5335828969591083 9 0.24649019595531346 1.4167756688114688 >10 1.2627020712532364 13.345467364221944 >50 0.029278450834829547 1.2778836047777322 >100 0.0189238767199252 2.054555899483828 >500 0.0021423256664066265 1.0179286942614658 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 936 0.2137596345989152 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 865 0.19754496146160436 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 773 0.1765343990864973 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 729 0.16648586925492437 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 615 0.14045104196403083 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 546 0.12469312018270054 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.135027119611762E-4 0.0 0.0 2.2837567799029404E-4 0.0 6 0.0011418783899514702 0.0 0.0 2.2837567799029404E-4 0.0 7 0.0015986297459320582 0.0 0.0 2.2837567799029404E-4 0.0 8 0.0020553811019126465 0.0 0.0 2.2837567799029404E-4 0.0 9 0.0020553811019126465 0.0 0.0 6.851270339708821E-4 0.0 10-11 0.0034256351698544107 0.0 0.0 0.0012560662289466172 0.0 12-13 0.004453325720810733 0.0 0.0 0.0015986297459320582 0.0 14-15 0.0047958892377961745 0.0 0.0 0.0018270054239223523 0.0 16-17 0.005709391949757351 0.0 0.0 0.0020553811019126465 0.0 18-19 0.00650870682272338 0.0 0.0 0.0022837567799029405 0.0 20-21 0.007422209534684556 0.0 0.0 0.0038823865258349986 0.0 22-23 0.008335712246645732 0.0 0.0 0.013245789323437052 0.0 24-25 0.009020839280616614 0.0 0.0 0.03574079360548102 0.0 26-27 0.010048529831572936 0.0 0.0 0.09763060234085069 0.0 28-29 0.011304596060519555 0.0 0.0 0.3405081358835284 0.0 30-31 0.012446474450471024 0.0 0.0 0.8440765058521267 0.0 32-33 0.012789037967456465 0.0 0.0 1.628889523265772 0.0 34-35 0.012903225806451613 0.0 0.0 2.7313731087639166 0.0 36-37 0.015186982586354553 0.0 0.0 4.2247216671424495 0.0 38-39 0.015872109620325436 0.0 0.0 6.330459606051956 0.0 40-41 0.01689980017128176 0.0 0.0 9.039794461889809 0.0 42-43 0.018612617756208965 0.0 0.0 11.179103625463888 0.0 44-45 0.019297744790179844 0.0 0.0 13.020839280616613 0.0 46-47 0.02032543534113617 0.0 0.0 15.115386811304596 0.0 48-49 0.02066799885812161 0.0 0.0 17.42380816443049 0.0 50-51 0.020896374536111904 0.0 0.0 19.767856123322865 0.0 52-53 0.0211247502141022 0.0 0.0 21.88101627176706 0.0 54-55 0.02146731373108764 0.0 0.0 23.82495004282044 0.0 56-57 0.021695689409077932 0.0 0.0 25.773451327433627 0.0 58-59 0.02180987724807308 0.0 0.0 27.660176991150443 0.0 60-61 0.021924065087068227 0.0 0.0 29.693748215815017 0.0 62-63 0.022495004282043964 0.0 0.0 31.696146160433912 0.0 64-65 0.022723379960034255 0.0 0.0 33.56562946046246 0.0 66-67 0.023294319155009992 0.0 0.0 35.510248358549816 0.0 68-69 0.023522694833000287 0.0 0.0 37.562203825292606 0.0 70-71 0.023522694833000287 0.0 0.0 39.805538110191264 0.0 72-73 0.023751070510990582 0.0 0.0 42.030373965172714 0.0 74-75 0.023979446188980874 0.0 0.0 43.976363117328006 0.0 76-77 0.023979446188980874 0.0 0.0 45.960034256351705 0.0 78-79 0.024322009705966315 0.0 0.0 47.962546388809585 0.0 80-81 0.02523551241792749 0.0 0.0 49.86491578646874 0.0 82-83 0.02603482729089352 0.0 0.0 51.885012846131886 0.0 84-85 0.026263202968883813 0.0 0.0 54.00045675135598 0.0 86-87 0.026263202968883813 0.0 0.0 55.73359977162433 0.0 88-89 0.026263202968883813 0.0 0.0 57.260519554667425 0.0 90-91 0.02637739080787896 0.0 0.0 59.04607479303454 0.0 92-93 0.026491578646874108 0.0 0.0 60.70762203825292 0.0 94-95 0.026491578646874108 0.0 0.0 62.197088210105626 0.0 96-97 0.026491578646874108 0.0 0.0 63.67513559805881 0.0 98-99 0.026491578646874108 0.0 0.0 65.00325435341136 0.0 100-101 0.026491578646874108 0.0 0.0 66.30122751926919 0.0 102-103 0.026491578646874108 0.0 0.0 67.61838424207822 0.0 104-105 0.026491578646874108 0.0 0.0 68.86257493576935 0.0 106-107 0.026491578646874108 0.0 0.0 70.0047958892378 0.0 108-109 0.026491578646874108 0.0 0.0 71.09688838138737 0.0 110-111 0.026491578646874108 0.0 0.0 72.15586640022838 0.0 112-113 0.026491578646874108 0.0 0.0 73.15683699685982 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGGG 55 1.9917934E-9 75.709755 3 TATAAGG 70 1.7462298E-10 67.984276 2 GTATAAG 80 5.638867E-10 59.601948 1 CTTAAGG 40 1.7727909E-4 59.486237 3 TCTTAAG 40 1.7727909E-4 59.486237 2 GCGGTCT 35 0.0078001763 50.98821 9 GGAAACA 130 0.0 50.432415 1 ACAAAGT 55 8.5323316E-4 43.26272 9 TTCCATA 55 8.5323316E-4 43.26272 2 GTCTAGG 110 4.6286732E-7 37.92851 1 ATACTGG 110 4.699723E-7 37.854877 6 CTTGGGC 40 1.198868E-4 37.178898 16-17 TACTGGT 115 6.65701E-7 36.209015 7 TACTAGG 165 2.3646862E-10 36.052265 2 CACCGAT 85 1.7110826E-4 34.991905 8 TTAATGG 120 9.287778E-7 34.70031 4 GTACTGG 155 5.0204108E-9 34.60758 1 TAAGGGC 105 1.4839432E-5 33.992138 4 TATACTG 105 1.4839432E-5 33.992138 5 CACAAAG 140 8.0626705E-8 33.992138 9 >>END_MODULE