FastQCFastQC Report
Thu 26 May 2016
SRR935949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437875
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT81991.8724521838424208No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT45401.036825578075935TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT27400.6257493576934057RNA PCR Primer, Index 3 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8620.19685983442763347No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT8250.18840993434199257No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7450.17013988010276906No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC7420.16945475306879818No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7320.16717099628889523No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG6970.15917784755923495TruSeq Adapter, Index 3 (95% over 24bp)
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6300.14387667713388524No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5310.12126748501284614No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4690.10710819297744789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA700.085.32411
AGGACCG359.12925E-568.025335
CTATACA401.7377503E-459.7268681
GATATGA150.004237632759.494976102-103
TATGTTT150.004237632759.49497614-15
CAGATGT453.1629356E-452.908595
GGACCGG453.17008E-452.8844226
GTGTTAG350.00767666451.194461
GTCTAGG1350.048.666341
TCCATAT255.3266203E-447.609573118-119
CAAGGGT909.5818905E-846.3532684
GTATACG951.4409306E-744.0092771
TATACGG951.4657962E-743.9136242
TCCTAGG558.454487E-443.3433152
CGAGTGG558.526164E-443.2690736
GTCCTAG600.001283381639.8179131
GAAGTAA600.001283381639.8179131
CGGAGAT600.001283381639.8179131
CCTAGGG600.001297186839.7313733
TAAGGGC1053.2161734E-739.7313734
Produced by FastQC (version 0.11.5)