##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437875 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33129089351984 33.0 31.0 34.0 30.0 34.0 2 32.434493862403656 34.0 31.0 34.0 31.0 34.0 3 32.426386525835 34.0 31.0 34.0 30.0 34.0 4 35.92502654867257 37.0 35.0 37.0 35.0 37.0 5 35.91690550956323 37.0 35.0 37.0 35.0 37.0 6 36.00195717956038 37.0 35.0 37.0 35.0 37.0 7 35.981492435055664 37.0 35.0 37.0 35.0 37.0 8 35.989021981159006 37.0 35.0 37.0 35.0 37.0 9 37.653476448758205 39.0 37.0 39.0 35.0 39.0 10-11 37.58109620325435 39.0 37.0 39.0 35.0 39.0 12-13 37.571261204681704 39.0 37.0 39.0 35.0 39.0 14-15 38.7011019126463 40.0 38.0 41.0 34.0 41.0 16-17 38.62074336283186 40.0 38.0 41.0 34.0 41.0 18-19 38.696377961747075 40.0 38.0 41.0 34.0 41.0 20-21 38.798103339994285 40.0 38.0 41.0 35.0 41.0 22-23 38.6168632600628 40.0 38.0 41.0 34.0 41.0 24-25 38.29896317442193 40.0 38.0 41.0 33.0 41.0 26-27 38.16414387667713 40.0 37.5 41.0 33.0 41.0 28-29 38.135743077362264 40.0 37.0 41.0 33.0 41.0 30-31 37.96586697116757 40.0 37.0 41.0 33.0 41.0 32-33 37.69562888952326 40.0 36.5 41.0 33.0 41.0 34-35 37.60423522694833 40.0 36.0 41.0 33.0 41.0 36-37 37.58948330002855 40.0 36.0 41.0 33.0 41.0 38-39 37.279593491293184 40.0 35.5 41.0 32.0 41.0 40-41 37.081079075078506 39.5 35.0 41.0 32.0 41.0 42-43 36.87166086211818 39.0 35.0 41.0 31.0 41.0 44-45 36.51445960605196 39.0 35.0 41.0 31.0 41.0 46-47 36.0881244647445 39.0 35.0 41.0 31.0 41.0 48-49 35.57203083071653 38.0 35.0 40.0 29.5 41.0 50-51 35.01031458749643 37.0 35.0 39.5 29.0 41.0 52-53 34.57338509848701 36.5 34.5 39.0 28.0 41.0 54-55 34.12810962032543 35.5 34.0 39.0 28.0 41.0 56-57 33.7502392235227 35.0 34.0 38.0 27.5 40.5 58-59 33.41120867827576 35.0 34.0 37.0 27.0 39.5 60-61 33.01772994576078 35.0 33.5 36.5 26.5 39.0 62-63 32.63802226662861 35.0 33.0 36.0 26.0 38.5 64-65 32.32265600913503 35.0 33.0 35.0 26.0 37.0 66-67 31.84804224950043 35.0 33.0 35.0 24.5 37.0 68-69 31.332722809020837 35.0 33.0 35.0 21.0 36.0 70-71 30.789074507564948 35.0 33.0 35.0 14.5 36.0 72-73 30.445058521267484 35.0 32.0 35.0 7.5 35.0 74-75 30.306223237225236 35.0 32.0 35.0 7.0 35.0 76-77 30.16633399942906 35.0 32.0 35.0 7.0 35.0 78-79 30.251542106765626 35.0 32.0 35.0 7.0 35.0 80-81 30.22568998001713 35.0 32.0 35.0 5.0 35.0 82-83 30.124543534113617 35.0 32.0 35.0 2.0 35.0 84-85 30.041897801884097 35.0 32.0 35.0 2.0 35.0 86-87 30.027413074507564 35.0 32.0 35.0 2.0 35.0 88-89 29.943184698829576 35.0 32.0 35.0 2.0 35.0 90-91 29.836895232657724 35.0 31.5 35.0 2.0 35.0 92-93 29.730282614901512 35.0 31.0 35.0 2.0 35.0 94-95 29.674024550385383 35.0 31.0 35.0 2.0 35.0 96-97 29.633973165857835 35.0 31.0 35.0 2.0 35.0 98-99 29.551239508992293 35.0 31.0 35.0 2.0 35.0 100-101 29.448316300314016 35.0 31.0 35.0 2.0 35.0 102-103 28.615874393377105 33.5 29.5 35.0 2.0 35.0 104-105 29.088859834427634 34.5 31.0 35.0 2.0 35.0 106-107 28.96492834713103 35.0 31.0 35.0 2.0 35.0 108-109 28.6654547530688 35.0 30.0 35.0 2.0 35.0 110-111 28.249524407650583 35.0 29.5 35.0 2.0 35.0 112-113 27.683204110762205 34.0 29.0 35.0 2.0 35.0 114-115 26.948631458749645 34.0 26.0 35.0 2.0 35.0 116-117 26.145029974307736 34.0 22.0 35.0 2.0 35.0 118-119 25.46925264059378 34.0 9.5 35.0 2.0 35.0 120-121 24.735880102769055 34.0 2.0 35.0 2.0 35.0 122-123 23.926509848701112 33.0 2.0 35.0 2.0 35.0 124-125 21.57077133885241 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.004877280981901322 1101 2 0.07873132297008567 1101 3 0.11032292981123959 1101 4 0.13013554433388208 1101 5 0.21573177567648827 1101 6 0.11780546115409152 1101 7 0.28031931029961044 1101 8 0.1792349214863691 1101 9 0.23452376524952 1101 10-11 0.09464594656072478 1101 12-13 0.27676383201441723 1101 14-15 0.4369066314188075 1101 16-17 0.3187511351705723 1101 18-19 0.4969843688997031 1101 20-21 0.3540879256121272 1101 22-23 0.4324882738599811 1101 24-25 0.5713615077798124 1101 26-27 0.26619601651470504 1101 28-29 0.355326121960843 1101 30-31 0.5013144222810197 1101 32-33 0.5998826063744289 1101 34-35 0.6098310588811771 1101 36-37 0.5370530864997747 1101 38-39 0.4920300280059351 1101 40-41 0.5483410760250962 1101 42-43 0.31208383085741787 1101 44-45 0.5635511978981569 1101 46-47 0.6785621582048833 1101 48-49 0.4422366367037256 1101 50-51 0.5826166646329369 1101 52-53 0.6844961831325094 1101 54-55 0.6704361372743861 1101 56-57 0.5568631987050594 1101 58-59 0.5820596608131439 1101 60-61 0.208786610480324 1101 62-63 0.40707022848823726 1101 64-65 0.24169565039968433 1101 66-67 0.10464280658244007 1101 68-69 0.18656570619438284 1101 70-71 0.47152686453806325 1101 72-73 0.48155794519880857 1101 74-75 0.6594805609390484 1101 76-77 0.41755473129768816 1101 78-79 0.29727785911141424 1101 80-81 0.29997025285546286 1101 82-83 0.38376702345123803 1101 84-85 0.484700201911501 1101 86-87 0.22110541146176743 1101 88-89 0.08687177208244279 1101 90-91 0.22925896456670003 1101 92-93 0.30719890043818054 1101 94-95 0.3047362309897217 1101 96-97 0.32490979342396287 1101 98-99 0.17522744573632565 1101 100-101 0.08414780110582853 1101 102-103 0.22660810580440582 1101 104-105 0.5767681462284528 1101 106-107 0.4931997924064895 1101 108-109 0.40452777975712095 1101 110-111 0.14906988898342632 1101 112-113 0.2728919816200168 1101 114-115 0.5740788349052082 1101 116-117 0.4606442427601998 1101 118-119 0.154891607578584 1101 120-121 -0.04924332407026455 1101 122-123 -0.2087426246453319 1101 124-125 -0.12543863335925565 1102 1 0.06551225502855829 1102 2 0.054789913906653 1102 3 0.14997073626676638 1102 4 0.25722719969560615 1102 5 0.29433318317796164 1102 6 0.3872236880956237 1102 7 0.3315429919601556 1102 8 0.37541737023810384 1102 9 0.3795693080169116 1102 10-11 0.2652295708336183 1102 12-13 0.39290584330104394 1102 14-15 0.40402154266175216 1102 16-17 0.35150027536824524 1102 18-19 0.43947812828231747 1102 20-21 0.3189958421570651 1102 22-23 0.32259121873718044 1102 24-25 0.21059163398056313 1102 26-27 0.3398512104727942 1102 28-29 0.256892133663996 1102 30-31 0.30299150684970044 1102 32-33 0.3290918774876772 1102 34-35 0.4185832535826606 1102 36-37 0.5524167199708927 1102 38-39 0.5680137026087166 1102 40-41 0.4424371482687377 1102 42-43 0.47599874441437606 1102 44-45 0.49340318751979595 1102 46-47 0.4490101231706518 1102 48-49 0.2827328200202288 1102 50-51 0.13830350857266183 1102 52-53 0.3039009598442348 1102 54-55 0.1142344243225395 1102 56-57 0.16019422766210312 1102 58-59 0.15935654064907823 1102 60-61 0.25041290235320446 1102 62-63 0.4009843610641113 1102 64-65 0.32652755145227985 1102 66-67 0.3996902294894831 1102 68-69 0.6809527662055466 1102 70-71 0.7174462383647828 1102 72-73 0.5339993788486836 1102 74-75 0.6755543086937266 1102 76-77 0.6543163787628252 1102 78-79 0.5295156013240181 1102 80-81 0.6455399207071011 1102 82-83 0.7819314286473684 1102 84-85 0.7529440647018006 1102 86-87 0.6726571051783417 1102 88-89 0.7538194955225777 1102 90-91 0.5899478477348197 1102 92-93 0.5264521089688081 1102 94-95 0.6944212063598911 1102 96-97 0.7806513060397009 1102 98-99 0.8632717823218172 1102 100-101 0.7743571908124984 1102 102-103 0.8609194974259253 1102 104-105 0.6568678804056276 1102 106-107 0.8626342174742447 1102 108-109 0.8373966703879532 1102 110-111 0.6855879860506988 1102 112-113 0.6576726850236483 1102 114-115 0.7408631308218325 1102 116-117 1.0539946226930361 1102 118-119 0.8828310612967769 1102 120-121 1.0710349944485351 1102 122-123 0.9641636952271639 1102 124-125 0.6891853820983833 1103 1 0.1481429464282229 1103 2 0.19306984645302805 1103 3 0.2713872624050495 1103 4 0.2707179248220797 1103 5 0.31119658958605356 1103 6 0.3416924907937684 1103 7 0.309620563629629 1103 8 0.29110033819763004 1103 9 0.349215176482339 1103 10-11 0.4448248490798221 1103 12-13 0.39880803554387256 1103 14-15 0.19322896256057476 1103 16-17 0.38607025850484433 1103 18-19 0.5617378247410088 1103 20-21 0.47919820303396676 1103 22-23 0.48195040929368105 1103 24-25 0.3674213135758464 1103 26-27 0.34406706207482074 1103 28-29 0.5283929768343114 1103 30-31 0.34346368222912815 1103 32-33 0.413408909528151 1103 34-35 0.561079037731055 1103 36-37 0.44364774863868206 1103 38-39 0.39769329788696695 1103 40-41 0.3893174180832375 1103 42-43 0.40938828910240943 1103 44-45 0.2640608603697032 1103 46-47 0.47514840310319784 1103 48-49 0.42775811512984774 1103 50-51 0.3516255996350566 1103 52-53 0.4371218704681823 1103 54-55 0.2171012034119144 1103 56-57 0.3372599949470967 1103 58-59 0.28245940742817055 1103 60-61 0.08346517891306604 1103 62-63 0.2728224723625985 1103 64-65 0.4116877538131547 1103 66-67 0.4055924217323117 1103 68-69 0.22226811190537532 1103 70-71 -0.01442559974651303 1103 72-73 -0.21389269535030664 1103 74-75 -0.33962275707355616 1103 76-77 -0.3650765386064805 1103 78-79 -0.43254173425777154 1103 80-81 -0.19594405905681356 1103 82-83 -0.11182911098163828 1103 84-85 -0.1627389032577291 1103 86-87 -0.09378471606954975 1103 88-89 -0.010935985084504551 1103 90-91 -0.3198329279818708 1103 92-93 -0.26106897028920173 1103 94-95 -0.3637575457480331 1103 96-97 -0.3812373954780064 1103 98-99 -0.4301514891790248 1103 100-101 -0.2947827754606891 1103 102-103 -0.1989455953228081 1103 104-105 -0.4131152561879645 1103 106-107 -0.24276207257634397 1103 108-109 0.14262432637445954 1103 110-111 0.2673755071299553 1103 112-113 -0.1526139928852892 1103 114-115 0.08403345122655637 1103 116-117 0.4781092938566083 1103 118-119 0.7917686666930663 1103 120-121 0.8939692271635415 1103 122-123 1.0071653815678054 1103 124-125 0.8915462589955219 1104 1 -0.05717779556165681 1104 2 0.03580576550867676 1104 3 0.20303785262427 1104 4 0.13227070728414247 1104 5 0.15839524051354203 1104 6 0.2231063187870248 1104 7 0.14428363276959288 1104 8 0.05285413246406989 1104 9 0.08772445135586793 1104 10-11 0.24385486594322714 1104 12-13 0.25478510131116394 1104 14-15 0.23962086812549188 1104 16-17 0.271175148892695 1104 18-19 0.2252643002890693 1104 20-21 0.0035273767270496137 1104 22-23 0.2727929050778286 1104 24-25 0.2745079914847821 1104 26-27 0.5387490182299715 1104 28-29 0.5065906159574212 1104 30-31 0.5215655372038341 1104 32-33 0.542392383389867 1104 34-35 0.5920362046787773 1104 36-37 0.639716551336825 1104 38-39 0.6130619319510444 1104 40-41 0.48503816007311684 1104 42-43 0.6702382047853703 1104 44-45 0.8137741824607687 1104 46-47 0.6593264806748707 1104 48-49 0.7736603073726798 1104 50-51 0.5568465102590068 1104 52-53 0.6711994421376559 1104 54-55 0.6520745592297885 1104 56-57 0.6303932158577226 1104 58-59 0.5913646350841546 1104 60-61 0.5388257185420713 1104 62-63 0.36857829023781363 1104 64-65 0.6258273828182155 1104 66-67 0.8493549849700877 1104 68-69 0.8117183648564783 1104 70-71 0.6993457324625929 1104 72-73 0.9569349606362039 1104 74-75 0.8790538027915353 1104 76-77 0.8151204259803642 1104 78-79 0.7225759723189604 1104 80-81 0.9722515564575218 1104 82-83 1.0477162515815976 1104 84-85 1.0627612653763343 1104 86-87 1.0365208488545683 1104 88-89 1.102871772082441 1104 90-91 1.2685097364363394 1104 92-93 1.1057141663043915 1104 94-95 1.1062552704408368 1104 96-97 1.0843814915371723 1104 98-99 1.2218566052560504 1104 100-101 1.1907646107113194 1104 102-103 1.2600736289604946 1104 104-105 1.1978823829351413 1104 106-107 1.3122611989244994 1104 108-109 1.2032529941653536 1104 110-111 0.9273930450726198 1104 112-113 0.9847876934553526 1104 114-115 0.7407875827611257 1104 116-117 0.9083217727773523 1104 118-119 1.2997012130674364 1104 120-121 1.3388609640944047 1104 122-123 1.5934021437937744 1104 124-125 1.3977654832788247 1105 1 0.3155445831550878 1105 2 0.37434222873246625 1105 3 0.2656632047369527 1105 4 0.3154969983639546 1105 5 0.2958875722819272 1105 6 0.25878206213600663 1105 7 0.4028601585911957 1105 8 0.3054988898975637 1105 9 0.46092006950922837 1105 10-11 0.41181981117014743 1105 12-13 0.37486147063823694 1105 14-15 0.5051307272804237 1105 16-17 0.5086624728363631 1105 18-19 0.4253619528712278 1105 20-21 0.37187542054552125 1105 22-23 0.4634019817601427 1105 24-25 0.526690777087282 1105 26-27 0.5045280479639231 1105 28-29 0.5598964062547509 1105 30-31 0.7160394026185415 1105 32-33 0.684793635346054 1105 34-35 0.5816687555395035 1105 36-37 0.38090215384073645 1105 38-39 0.48737679893070407 1105 40-41 0.5239502101513693 1105 42-43 0.4280910999340435 1105 44-45 0.3176239477189853 1105 46-47 0.45916247441508773 1105 48-49 0.36323777216141195 1105 50-51 0.0513566824029823 1105 52-53 0.1139474859561247 1105 54-55 0.13501415234403424 1105 56-57 0.1983994756386238 1105 58-59 0.3607568103580192 1105 60-61 0.35672493606945466 1105 62-63 0.25384652184970236 1105 64-65 0.39757949549426996 1105 66-67 0.36144011799042985 1105 68-69 0.18806891258730474 1105 70-71 -0.18598634891455745 1105 72-73 -0.08472137739196484 1105 74-75 -0.11833211270142385 1105 76-77 -0.180820732079102 1105 78-79 -0.0408831826106173 1105 80-81 0.057509772419962246 1105 82-83 -0.03700863104750951 1105 84-85 -0.02956549518704321 1105 86-87 0.16791740597506433 1105 88-89 0.0673099567459765 1105 90-91 0.15003461906544757 1105 92-93 0.25179366552191595 1105 94-95 0.26133383069122473 1105 96-97 0.07252546649804614 1105 98-99 0.1084944769305416 1105 100-101 0.06278213809785882 1105 102-103 0.23008427058803704 1105 104-105 0.1363850433420275 1105 106-107 0.11404148061463815 1105 108-109 0.24610995817161552 1105 110-111 0.32789507950141683 1105 112-113 0.49915138672608705 1105 114-115 0.1715080835435998 1105 116-117 0.1874015849839239 1105 118-119 -0.2420703049294346 1105 120-121 -0.15108958363642344 1105 122-123 -0.28654026622187345 1105 124-125 -0.09648177806702662 1106 1 -0.04162116905563806 1106 2 0.015331066713500263 1106 3 -0.01687540038777513 1106 4 0.08022492415160798 1106 5 0.0391446381038989 1106 6 0.022920776618349237 1106 7 -0.0032007045798039258 1106 8 0.011348108367684517 1106 9 -0.057347837800762136 1106 10-11 -0.02046802562303185 1106 12-13 0.11654534227501756 1106 14-15 0.15654978378814377 1106 16-17 0.05731129347101671 1106 18-19 0.04364140872280586 1106 20-21 0.12869966588367276 1106 22-23 0.14270133881276337 1106 24-25 0.008280280641407955 1106 26-27 -0.013942548035096536 1106 28-29 0.153582182222479 1106 30-31 0.25687999503515613 1106 32-33 0.19485382917299887 1106 34-35 0.11205909624504073 1106 36-37 0.35067679230068194 1106 38-39 0.16472940183056295 1106 40-41 0.21537671428998806 1106 42-43 0.2501598915323697 1106 44-45 0.12320189330414166 1106 46-47 0.388265034891738 1106 48-49 0.04058801821605584 1106 50-51 0.3282200042349075 1106 52-53 0.07031992406536602 1106 54-55 -0.0313013443846657 1106 56-57 0.09056791465290104 1106 58-59 -0.014054642024277086 1106 60-61 -0.09732247422900997 1106 62-63 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50-51 -1.9486977935384644 2216 52-53 -1.8181866338117132 2216 54-55 -1.882262149630968 2216 56-57 -1.642189062623288 2216 58-59 -1.1881910611183741 2216 60-61 -1.3811603574072961 2216 62-63 -1.4267647477368754 2216 64-65 -1.4337852754096332 2216 66-67 -1.6844880530045963 2216 68-69 -1.500395559194157 2216 70-71 -1.3688719890563945 2216 72-73 -1.0605637735410127 2216 74-75 -1.269149994676816 2216 76-77 -1.3299795740196352 2216 78-79 -1.3647265593266091 2216 80-81 -1.4624952789855143 2216 82-83 -1.492741976266501 2216 84-85 -1.6318564561426498 2216 86-87 -1.9393791511454346 2216 88-89 -2.252526962094773 2216 90-91 -2.078567478753534 2216 92-93 -2.5716238083791545 2216 94-95 -2.4961219447490386 2216 96-97 -2.5766754704881443 2216 98-99 -2.9557054200604043 2216 100-101 -2.8304227310608354 2216 102-103 -2.863473206482542 2216 104-105 -2.6041720474446066 2216 106-107 -2.755509687151452 2216 108-109 -2.6105242210245194 2216 110-111 -2.546166448598264 2216 112-113 -2.4711629394560397 2216 114-115 -2.5161503919224195 2216 116-117 -2.0997225310201166 2216 118-119 -1.7400076476920603 2216 120-121 -1.7205365042600285 2216 122-123 -1.7288155777252108 2216 124-125 -1.8441838838097837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 25.0 5 83.0 6 211.0 7 507.0 8 751.0 9 918.0 10 1275.0 11 2113.0 12 2831.0 13 3064.0 14 4111.0 15 5036.0 16 2879.0 17 2021.0 18 2255.0 19 3036.0 20 4676.0 21 6609.0 22 6438.0 23 2197.0 24 2914.0 25 3672.0 26 4802.0 27 5989.0 28 7207.0 29 9142.0 30 11635.0 31 15451.0 32 20927.0 33 28345.0 34 39593.0 35 58921.0 36 126793.0 37 47582.0 38 3778.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.98423776985497 19.869380357625047 11.405190917953107 16.741190954566882 2 18.912974965401006 20.803016392843602 37.43702526297519 22.846983378780198 3 28.997544961461603 22.96157579217813 27.419469026548672 20.62141021981159 4 16.019151096112164 22.28441298323593 27.098709541708963 34.59772637894294 5 15.909729503758987 33.76965443825371 27.263901167558725 23.056714890428577 6 32.0367418342598 35.1941004782285 9.52620208008843 23.242955607423276 7 28.35695118469883 38.89603197259492 11.656066228946617 21.090950613759635 8 36.142278047387954 27.30071367399372 12.509734513274337 24.04727376534399 9 34.69962888952327 13.873822437910363 16.098658292891805 35.327890379674564 10-11 29.48535540964887 25.484784470453896 17.662003996574366 27.367856123322866 12-13 33.1318298601199 25.552383671139022 19.053040251213247 22.262746217527834 14-15 35.710876391664286 19.475078504139308 21.72914644590351 23.08489865829289 16-17 31.132172423636884 21.700256922637738 22.922295175563804 24.245275478161577 18-19 30.506423065943476 21.782586354553242 22.57128175849272 25.139708821010565 20-21 37.3012846131887 19.318298601198972 19.777562089637453 23.60285469597488 22-23 32.60976306023409 23.338167285184127 19.416956894090777 24.63511276049101 24-25 28.03596916928347 24.558035969169286 21.08775335426777 26.318241507279478 26-27 26.10002854695975 23.267370825007134 23.73622609192121 26.896374536111907 28-29 27.983214387667715 20.797602055381102 25.72891807022552 25.490265486725665 30-31 28.61318869540394 22.981216100485298 23.76899800171282 24.636597202397944 32-33 25.96448758207251 22.158035969169283 25.20570939194976 26.67176705680845 34-35 25.353811019126464 21.773337139594634 25.62066799885812 27.25218384242078 36-37 25.3670568084499 24.06497288038824 23.049957179560376 27.51801313160148 38-39 26.512132457893234 18.980645161290322 25.262232372252356 29.244990008564088 40-41 24.50288324293463 19.017071081929775 27.072566371681418 29.40747930345418 42-43 26.264459035112758 18.850699400513847 28.03037396517271 26.85446759920069 44-45 25.363060234085072 18.872851841278905 28.711390236939767 27.05269768769626 46-47 24.143419925777902 18.633057379389093 27.54918641164716 29.67433628318584 48-49 22.551184698829573 19.43602626320297 28.429232086782758 29.583556951184697 50-51 22.866228946617184 19.81341707108193 28.039394804453327 29.28095917784756 52-53 22.333428489865828 20.366657151013417 27.822666286040537 29.477248073080215 54-55 20.30488153011704 20.332857550670855 26.584870111333142 32.77739080787896 56-57 21.2681701398801 20.67085355409649 26.106651441621466 31.95432486440194 58-59 21.049957179560376 20.877076791321723 26.867370825007136 31.205595204110764 60-61 22.020097059663147 22.825007136739934 23.555352554952897 31.599543248644018 62-63 20.37042534970026 24.242763345703683 23.487981729945762 31.8988295746503 64-65 19.613245789323436 24.1506137596346 23.134341992577788 33.101798458464174 66-67 20.543419925777904 24.808107336568654 22.33388524122181 32.31458749643163 68-69 21.17270910648016 24.286839851555808 22.438138738224378 32.10231230373965 70-71 21.4258635455324 24.085069940051383 22.8446474450471 31.64441906936911 72-73 22.325092777619187 23.078275763631172 23.311904082215243 31.2847273765344 74-75 22.719383385669424 22.605195546674278 23.431801313160147 31.243619754496144 76-77 22.264801598629745 23.296146160433914 23.296602911789893 31.142449329146444 78-79 22.577790465315445 23.52874678846703 23.82495004282044 30.068512703397086 80-81 22.955638024550385 24.282729089351985 23.281644304881528 29.479988581216098 82-83 21.647387953182985 24.097516414501854 23.91276049100771 30.34233514130745 84-85 21.614045104196403 22.765972023979447 24.633057379389093 30.98692549243506 86-87 22.076620039965743 22.537253782472167 24.647673422780475 30.738452754781616 88-89 22.22711961176135 22.844875820725093 24.484384813017414 30.443619754496147 90-91 22.277819012275195 21.933771053382813 24.802512132457892 30.9858978018841 92-93 22.078561233228662 21.87576363117328 24.85812161004853 31.18755352554953 94-95 21.634713103054526 21.439337710533827 25.243277190979164 31.682671995432486 96-97 21.492549243505565 21.396631458749642 25.46194690265487 31.64887239508992 98-99 22.006166143305737 21.957065372537823 25.3905795032829 30.646188980873536 100-101 22.421010562375105 22.542734798743933 24.8823294319155 30.15392520696546 102-103 22.09317727662004 23.52337996003426 24.620610904938626 29.76283185840708 104-105 21.826662860405367 25.184813017413642 24.610219811590063 28.378304310590924 106-107 21.34638880959178 27.567684841564372 23.740108478447045 27.345817870396804 108-109 20.833799600342566 28.893976591493004 23.23151584356266 27.04070796460177 110-111 20.715276707260674 30.26748654003163 22.974496000548108 26.042740752159588 112-113 20.60736511561519 31.63220097059663 22.443619754496147 25.316814159292033 114-115 20.086600749306587 32.309593111220984 22.20959802133731 25.394208118135115 116-117 19.87285629129544 32.80990565283624 22.19350875069373 25.123729305174592 118-119 19.721487910017412 33.439036227126095 22.360786776670757 24.47868908618573 120-121 19.819262250155298 33.81513318960792 22.1553330653707 24.210271494866078 122-123 19.7663424404437 33.86847027494901 21.92763489950053 24.437552385106756 124-125 19.19779413948367 35.226377120822036 21.731129909558806 23.844698830135492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 3.0 10 3.5 11 2.5 12 3.0 13 3.0 14 3.0 15 4.5 16 7.0 17 9.5 18 9.0 19 12.5 20 23.5 21 41.5 22 60.5 23 91.5 24 138.5 25 194.0 26 264.0 27 359.5 28 493.0 29 626.5 30 763.5 31 929.0 32 1110.0 33 1230.0 34 1340.0 35 1463.5 36 1534.5 37 1521.5 38 1589.5 39 1753.0 40 1924.0 41 2152.0 42 2499.5 43 2892.0 44 3220.5 45 3569.0 46 4055.5 47 4878.5 48 6119.5 49 8628.0 50 13767.0 51 22532.0 52 34230.5 53 44224.0 54 48133.5 55 46973.5 56 42223.0 57 33776.0 58 24476.0 59 16990.5 60 11673.5 61 8211.5 62 6235.0 63 5145.0 64 4257.0 65 3603.5 66 3083.0 67 2752.0 68 2624.0 69 2272.0 70 1846.0 71 1332.0 72 746.0 73 441.5 74 291.0 75 174.0 76 81.0 77 76.0 78 84.5 79 57.0 80 16.0 81 7.5 82 5.0 83 3.0 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20119897230944905 2 2.2837567799029401E-4 3 0.0 4 0.11669997145304024 5 0.02831858407079646 6 0.0020553811019126465 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 5.709391949757351E-4 112-113 0.0 114-115 7.993148729660291E-4 116-117 0.005481016271767057 118-119 0.0028546959748786756 120-121 6.851270339708821E-4 122-123 0.0027405081358835284 124-125 0.030145589494718815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 437875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.23076744858698 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38724354492443 46.57854408601082 2 9.301389392547492 10.646513065472785 3 3.1775712752212866 5.455645281104983 4 1.6853196007984164 3.8580853659935848 5 1.018454895753399 2.9143477647868834 6 0.7092791067989581 2.43555525704116 7 0.4956943543312167 1.985827782281514 8 0.40241005556154547 1.842418904705262 9 0.28346544695151044 1.46006505667725 >10 1.4666123777893527 13.766772656514261 >50 0.0442535954692259 1.6647153258672966 >100 0.023920862411147915 2.5445319779572544 >500 0.0031894483214863884 1.3151616141450515 >1k 7.973620803715971E-4 1.66106463410409 >5k 3.9868104018579855E-4 1.8707512273378344 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 8199 1.8724521838424208 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 4540 1.036825578075935 TruSeq Adapter, Index 3 (95% over 21bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 2740 0.6257493576934057 RNA PCR Primer, Index 3 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 862 0.19685983442763347 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 825 0.18840993434199257 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 745 0.17013988010276906 No Hit TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC 742 0.16945475306879818 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 732 0.16717099628889523 No Hit CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG 697 0.15917784755923495 TruSeq Adapter, Index 3 (95% over 24bp) ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 630 0.14387667713388524 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 531 0.12126748501284614 No Hit TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 469 0.10710819297744789 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.567513559805881E-4 0.0 0.0 0.0 0.0 6 4.567513559805881E-4 0.0 0.0 0.0 0.0 7 6.851270339708821E-4 0.0 0.0 0.0 0.0 8 0.0011418783899514702 0.0 0.0 0.0 0.0 9 0.0011418783899514702 0.0 0.0 0.0 0.0 10-11 0.0022837567799029405 0.0 0.0 0.0 0.0 12-13 0.0034256351698544107 0.0 0.0 0.0 0.0 14-15 0.0038823865258349986 0.0 0.0 2.2837567799029404E-4 0.0 16-17 0.004910077076791322 0.0 0.0 4.567513559805881E-4 0.0 18-19 0.005481016271767057 0.0 0.0 6.851270339708821E-4 0.0 20-21 0.005823579788752498 0.0 0.0 0.0022837567799029405 0.0 22-23 0.005937767627747645 0.0 0.0 0.011647159577504996 0.0 24-25 0.006166143305737939 0.0 0.0 0.0342563516985441 0.0 26-27 0.006737082500713674 0.0 0.0 0.09648872395089922 0.0 28-29 0.007536397373679704 0.0 0.0 0.340165572366543 0.0 30-31 0.008221524407650586 0.0 0.0 0.8452183842420783 0.0 32-33 0.00844990008564088 0.0 0.0 1.631173280045675 0.0 34-35 0.008678275763631173 0.0 0.0 2.7373108763916645 0.0 36-37 0.009020839280616614 0.0 0.0 4.231001998287182 0.0 38-39 0.009477590636597203 0.0 0.0 6.3446188980873535 0.0 40-41 0.009591778475592349 0.0 0.0 9.07176705680845 0.0 42-43 0.010391093348558379 0.0 0.0 11.228889523265773 0.0 44-45 0.011190408221524407 0.0 0.0 13.078732514987154 0.0 46-47 0.011647159577504996 0.0 0.0 15.183899514701684 0.0 48-49 0.011989723094490437 0.0 0.0 17.50636597202398 0.0 50-51 0.01221809877248073 0.0 0.0 19.86868398515558 0.0 52-53 0.012560662289466172 0.0 0.0 21.998401370254065 0.0 54-55 0.012560662289466172 0.0 0.0 23.95626605766486 0.0 56-57 0.01267485012846132 0.0 0.0 25.91858407079646 0.0 58-59 0.012903225806451613 0.0 0.0 27.82437910362546 0.0 60-61 0.01301741364544676 0.0 0.0 29.885583785326865 0.0 62-63 0.01301741364544676 0.0 0.0 31.92554952897516 0.0 64-65 0.013245789323437054 0.0 0.0 33.82791892663431 0.0 66-67 0.013702540679417643 0.0 0.0 35.803140165572366 0.0 68-69 0.013702540679417643 0.0 0.0 37.89620325435341 0.0 70-71 0.013930916357407936 0.0 0.0 40.171281758492725 0.0 72-73 0.014045104196403084 0.0 0.0 42.41724236368827 0.0 74-75 0.014387667713388525 0.0 0.0 44.383442763345705 0.0 76-77 0.014616043391378819 0.0 0.0 46.389608906651446 0.0 78-79 0.015186982586354553 0.0 0.0 48.41210391093348 0.0 80-81 0.01575792178133029 0.0 0.0 50.34244932914645 0.0 82-83 0.015872109620325436 0.0 0.0 52.39349129317728 0.0 84-85 0.01598629745932058 0.0 0.0 54.546046246074795 0.0 86-87 0.01598629745932058 0.0 0.0 56.31150442477876 0.0 88-89 0.01598629745932058 0.0 0.0 57.86525834998572 0.0 90-91 0.016214673137310877 0.0 0.0 59.688609763060235 0.0 92-93 0.016443048815301172 0.0 0.0 61.376534399086495 0.0 94-95 0.016443048815301172 0.0 0.0 62.89534684556095 0.0 96-97 0.016443048815301172 0.0 0.0 64.40022837567798 0.0 98-99 0.016443048815301172 0.0 0.0 65.75598058806737 0.0 100-101 0.016443048815301172 0.0 0.0 67.08832429346275 0.0 102-103 0.016443048815301172 0.0 0.0 68.43105909220668 0.0 104-105 0.016443048815301172 0.0 0.0 69.70242649157865 0.0 106-107 0.016443048815301172 0.0 0.0 70.870568084499 0.0 108-109 0.016443048815301172 0.0 0.0 71.99109334855838 0.0 110-111 0.016443048815301172 0.0 0.0 73.08147302312304 0.0 112-113 0.016443048815301172 0.0 0.0 74.11007707679133 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 70 0.0 85.3241 1 AGGACCG 35 9.12925E-5 68.02533 5 CTATACA 40 1.7377503E-4 59.726868 1 GATATGA 15 0.0042376327 59.494976 102-103 TATGTTT 15 0.0042376327 59.494976 14-15 CAGATGT 45 3.1629356E-4 52.90859 5 GGACCGG 45 3.17008E-4 52.884422 6 GTGTTAG 35 0.007676664 51.19446 1 GTCTAGG 135 0.0 48.66634 1 TCCATAT 25 5.3266203E-4 47.609573 118-119 CAAGGGT 90 9.5818905E-8 46.353268 4 GTATACG 95 1.4409306E-7 44.009277 1 TATACGG 95 1.4657962E-7 43.913624 2 TCCTAGG 55 8.454487E-4 43.343315 2 CGAGTGG 55 8.526164E-4 43.269073 6 GTCCTAG 60 0.0012833816 39.817913 1 GAAGTAA 60 0.0012833816 39.817913 1 CGGAGAT 60 0.0012833816 39.817913 1 CCTAGGG 60 0.0012971868 39.731373 3 TAAGGGC 105 3.2161734E-7 39.731373 4 >>END_MODULE