FastQCFastQC Report
Thu 26 May 2016
SRR935948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447083
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7730.17289854456555045No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7660.17133283976353383No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7080.1583598571182532No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6620.14807093984785824No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6570.14695257927498923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC150.004238893759.49060474-75
TTGCACA350.007787682651.009062
TAGGGTC505.330296E-447.597815
TGTAGGG1550.046.0727042
TGTTAGG1056.359187E-945.3413932
GTGTTAG1109.451469E-943.3531721
GTTAGAG558.445217E-443.3531721
GTGCGAG558.445217E-443.3531721
GTAATAG853.4319764E-642.078081
TTAGAGG600.001306512739.6737172
ATAGGGT758.177446E-539.6692774
ACACGGT905.1539973E-639.66046
ACTAATC758.1882354E-539.66048
GGAAACA2000.038.74691
TAGGGCA957.464383E-637.5772175
GTTCTAG650.001920042436.6834561
TTCTAGG650.001935943836.6218952
TATATGG1951.8189894E-1236.621892
GGGTCAT1304.1987732E-836.6096047
GGTCATG1156.6536086E-736.211678
Produced by FastQC (version 0.11.5)