##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935948_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 447083 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.49488126365798 31.0 31.0 34.0 22.0 34.0 2 29.483661870390957 31.0 31.0 34.0 22.0 34.0 3 29.478503991428884 33.0 31.0 34.0 18.0 34.0 4 32.715826815155125 37.0 35.0 37.0 22.0 37.0 5 32.86121145290696 37.0 35.0 37.0 24.0 37.0 6 33.014625919571984 37.0 35.0 37.0 25.0 37.0 7 32.999961975740526 37.0 35.0 37.0 25.0 37.0 8 32.87473914239638 37.0 35.0 37.0 23.0 37.0 9 34.2216277514466 39.0 35.0 39.0 17.0 39.0 10-11 34.18510656857899 39.0 35.0 39.0 19.5 39.0 12-13 34.11548638619675 39.0 35.0 39.0 19.5 39.0 14-15 35.137851360932984 40.0 36.0 41.0 18.5 41.0 16-17 35.128736722264094 40.0 36.0 41.0 21.5 41.0 18-19 35.22842738372964 40.0 36.0 41.0 23.0 41.0 20-21 35.334577695864084 40.0 36.0 41.0 24.0 41.0 22-23 35.098384416316435 40.0 35.0 41.0 23.0 41.0 24-25 34.859107816669386 40.0 35.0 41.0 21.5 41.0 26-27 34.69837032497321 40.0 35.0 41.0 20.0 41.0 28-29 34.5525976161026 40.0 35.0 41.0 17.0 41.0 30-31 34.39342247412672 40.0 35.0 41.0 14.0 41.0 32-33 34.111224314053544 39.0 34.5 41.0 8.5 41.0 34-35 33.932470033528446 39.0 34.0 41.0 2.0 41.0 36-37 33.85268395353883 39.0 34.5 41.0 2.0 41.0 38-39 33.75604753479779 39.0 34.0 41.0 2.0 41.0 40-41 33.54866881541012 39.0 34.0 41.0 2.0 41.0 42-43 33.314575817018316 39.0 34.0 41.0 2.0 41.0 44-45 33.032279241214724 38.0 33.5 41.0 2.0 41.0 46-47 32.72280874021155 37.5 33.0 40.0 2.0 41.0 48-49 32.360046121190024 37.0 33.0 40.0 2.0 41.0 50-51 31.99076569674982 36.0 33.0 39.5 2.0 41.0 52-53 31.63695779083526 35.5 33.0 39.0 2.0 41.0 54-55 31.24538396673548 35.0 32.0 38.5 2.0 41.0 56-57 30.891757235233726 35.0 32.0 37.0 2.0 40.0 58-59 30.53555380097208 35.0 32.0 37.0 2.0 39.5 60-61 30.215303422407025 35.0 31.0 36.0 2.0 39.0 62-63 29.86671378692547 35.0 31.0 35.5 2.0 38.5 64-65 29.59124144733752 35.0 31.0 35.0 2.0 37.0 66-67 29.392606070908535 35.0 31.0 35.0 2.0 36.5 68-69 29.237054417188755 35.0 31.0 35.0 2.0 36.0 70-71 28.98975134371023 35.0 30.5 35.0 2.0 35.5 72-73 28.766352109116205 35.0 30.0 35.0 2.0 35.0 74-75 28.580391113059544 35.0 30.0 35.0 2.0 35.0 76-77 28.40840626908203 35.0 30.0 35.0 2.0 35.0 78-79 28.23455376294782 34.5 29.0 35.0 2.0 35.0 80-81 28.02219945737145 34.0 29.0 35.0 2.0 35.0 82-83 27.797218860927387 34.0 29.0 35.0 2.0 35.0 84-85 27.584454117020776 34.0 28.5 35.0 2.0 35.0 86-87 27.363136151452863 34.0 27.0 35.0 2.0 35.0 88-89 27.05307962950951 34.0 26.0 35.0 2.0 35.0 90-91 26.686949626803077 34.0 25.0 35.0 2.0 35.0 92-93 26.29615865510431 34.0 23.5 35.0 2.0 35.0 94-95 25.937588769870473 33.0 21.5 35.0 2.0 35.0 96-97 25.60419877293478 33.0 19.5 35.0 2.0 35.0 98-99 25.222715916284002 33.0 18.5 35.0 2.0 35.0 100-101 24.91435706569026 32.5 16.0 35.0 2.0 35.0 102-103 24.143909520156214 31.0 15.0 34.0 2.0 35.0 104-105 24.587763345955896 32.0 15.0 35.0 2.0 35.0 106-107 24.612737455908636 32.0 14.0 35.0 2.0 35.0 108-109 24.42443908625468 32.0 7.5 35.0 2.0 35.0 110-111 24.081813667708232 32.0 3.5 35.0 2.0 35.0 112-113 23.637008117061036 32.0 2.0 35.0 2.0 35.0 114-115 23.054654281196107 31.5 2.0 35.0 2.0 35.0 116-117 22.349267361988712 31.0 2.0 35.0 2.0 35.0 118-119 21.443612259915945 30.5 2.0 35.0 2.0 35.0 120-121 20.45372223949468 29.5 2.0 34.5 2.0 35.0 122-123 19.43199473028498 28.0 2.0 34.0 2.0 35.0 124-125 16.798028553982146 14.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010701297673751498 1101 2 0.2262588397567349 1101 3 0.0377870868849115 1101 4 0.02425581919656139 1101 5 0.07954049621727677 1101 6 0.09318793926107816 1101 7 0.08361543175935537 1101 8 -0.1713380982981647 1101 9 -0.05732933891157188 1101 10-11 0.07565216978949252 1101 12-13 0.019572861433466926 1101 14-15 -0.01598247079503068 1101 16-17 0.08133030560399135 1101 18-19 0.15794763601314088 1101 20-21 -0.01412384878356221 1101 22-23 0.13997165688994073 1101 24-25 0.20285982957441462 1101 26-27 0.2384418926609655 1101 28-29 0.18896614495217534 1101 30-31 0.017345541886804483 1101 32-33 -0.16839018742140865 1101 34-35 -0.30448294468489934 1101 36-37 -0.2991932059385931 1101 38-39 -0.2341617437943242 1101 40-41 -0.4405603767485218 1101 42-43 -0.3988526367020242 1101 44-45 -0.19424477831642406 1101 46-47 -0.13863235019443465 1101 48-49 -0.07953261991174543 1101 50-51 -0.07987013863667869 1101 52-53 0.13622806779092045 1101 54-55 -0.07577133720606355 1101 56-57 -0.2631269163124408 1101 58-59 -0.1406492509361641 1101 60-61 -0.02570443566895264 1101 62-63 0.035914803668429585 1101 64-65 -0.05707255490491647 1101 66-67 -0.09972892780378473 1101 68-69 -0.12152401668354784 1101 70-71 -0.4031929284035698 1101 72-73 -0.3547052423903807 1101 74-75 -0.21800883882673006 1101 76-77 -0.2119936399544784 1101 78-79 0.051478720078694806 1101 80-81 -0.10997772298159347 1101 82-83 -0.341730180130412 1101 84-85 -0.4225444791409707 1101 86-87 -0.37728499382804515 1101 88-89 -0.37052404142283635 1101 90-91 -0.5973442378161202 1101 92-93 -0.5385720022694223 1101 94-95 -0.4737248951484929 1101 96-97 -0.5987689513819525 1101 98-99 -0.9835799548308373 1101 100-101 -1.0757321564015285 1101 102-103 -0.9639529648231644 1101 104-105 -0.8260188862647162 1101 106-107 -0.416509291025708 1101 108-109 -0.47813099784931623 1101 110-111 -0.8692449552829125 1101 112-113 -0.6679816309037534 1101 114-115 -0.6984746852733181 1101 116-117 -0.7059272807525723 1101 118-119 -0.7302836391196301 1101 120-121 -1.0266852214545565 1101 122-123 -0.7154593730702103 1101 124-125 -0.5452199054863947 1102 1 0.07340118854499167 1102 2 0.10724548471526063 1102 3 0.09853878997403598 1102 4 0.23848590697842553 1102 5 0.21608250070843837 1102 6 0.3184065118165833 1102 7 0.2616288850923212 1102 8 0.28781422579157123 1102 9 0.17112025141452847 1102 10-11 0.387861233273199 1102 12-13 0.3339645001038605 1102 14-15 0.17192943986555775 1102 16-17 0.40961128821648174 1102 18-19 0.49361182186975583 1102 20-21 0.4037372275334761 1102 22-23 0.5058919802941588 1102 24-25 0.4241908475970462 1102 26-27 0.31627993348469374 1102 28-29 0.3453869736754669 1102 30-31 0.1722642749850749 1102 32-33 0.20661579827843468 1102 34-35 0.2778714641925859 1102 36-37 0.2805010319116974 1102 38-39 0.21086432865406124 1102 40-41 0.3839956103374931 1102 42-43 0.15921117211851055 1102 44-45 0.22590472556357355 1102 46-47 0.29153280471548015 1102 48-49 0.28973717511269115 1102 50-51 0.13876164379132305 1102 52-53 0.3124936967682608 1102 54-55 0.5673814002119926 1102 56-57 0.4369882611541094 1102 58-59 0.428604666231827 1102 60-61 0.33291081362598973 1102 62-63 0.5233523948715941 1102 64-65 0.40787392663770916 1102 66-67 0.3796916060450357 1102 68-69 0.5704543894316956 1102 70-71 0.6271766224043134 1102 72-73 0.35155693378325736 1102 74-75 0.41957530751165706 1102 76-77 0.5090551050647889 1102 78-79 0.540453691032301 1102 80-81 0.5171686229691765 1102 82-83 0.5858781358338874 1102 84-85 0.4736193803374782 1102 86-87 0.43700079246609036 1102 88-89 0.5679908497382407 1102 90-91 0.5375016083602127 1102 92-93 0.4026307831602516 1102 94-95 0.3627344436774038 1102 96-97 0.13607210623450428 1102 98-99 0.3286328890948731 1102 100-101 0.2735769048139076 1102 102-103 0.2377651200553217 1102 104-105 0.18532748035247337 1102 106-107 0.0486375539434043 1102 108-109 0.5414340496960435 1102 110-111 0.7545493987529603 1102 112-113 0.6565403322041732 1102 114-115 0.3425128669912958 1102 116-117 0.4732581096032682 1102 118-119 0.1910945084133786 1102 120-121 -0.10555028235747699 1102 122-123 -0.2580296703893765 1102 124-125 -0.4746466140571606 1103 1 0.4255803390021491 1103 2 0.36954578685393 1103 3 0.305281616649129 1103 4 0.0057848267833193745 1103 5 0.32715301947855835 1103 6 0.21824161914492635 1103 7 0.3163100596810722 1103 8 0.3047922466845563 1103 9 0.09310725747705106 1103 10-11 0.36554271395014126 1103 12-13 0.318826711047997 1103 14-15 0.14284735886080568 1103 16-17 0.3691298904795559 1103 18-19 0.3702311271482941 1103 20-21 0.5992634030108732 1103 22-23 0.39419447441923694 1103 24-25 0.45479102730043053 1103 26-27 0.13718501652345338 1103 28-29 0.22470800094693288 1103 30-31 0.4584011951454201 1103 32-33 0.27417489304728804 1103 34-35 0.3182701687483629 1103 36-37 0.44944690367061213 1103 38-39 0.28600527732998415 1103 40-41 0.3781626555003115 1103 42-43 0.24292999555498085 1103 44-45 0.2801946503251145 1103 46-47 0.3661911707969594 1103 48-49 0.28702557930708394 1103 50-51 0.2676815474229066 1103 52-53 0.3915014773326497 1103 54-55 0.5131161599821326 1103 56-57 0.43113802714475113 1103 58-59 0.5085590985863391 1103 60-61 0.4278586305260106 1103 62-63 0.3524292921247003 1103 64-65 0.3070240382117895 1103 66-67 0.3642390892101801 1103 68-69 0.4764877826661582 1103 70-71 0.2885554509751742 1103 72-73 0.4130078411866407 1103 74-75 0.48416238466563044 1103 76-77 0.4499381201322272 1103 78-79 0.4854997523684226 1103 80-81 0.34978281361275165 1103 82-83 0.6475721858743739 1103 84-85 0.6122464760542208 1103 86-87 0.7387926426211102 1103 88-89 0.5178997638163203 1103 90-91 0.6195480547750307 1103 92-93 0.7303105359199797 1103 94-95 0.7398622503086543 1103 96-97 0.48005228949246614 1103 98-99 0.6468263763280326 1103 100-101 0.8033884137299623 1103 102-103 0.6770436405632267 1103 104-105 0.743928558572815 1103 106-107 0.5882784433764492 1103 108-109 0.715700672259679 1103 110-111 0.43445026567371414 1103 112-113 0.8305613436775445 1103 114-115 0.33191579753889755 1103 116-117 0.31213600016192444 1103 118-119 0.6064068171082582 1103 120-121 0.47337357079918263 1103 122-123 0.7846313714953368 1103 124-125 0.44083354945317765 1104 1 0.9284572089676253 1104 2 1.0439089073650187 1104 3 1.04454791560552 1104 4 0.9808751009787855 1104 5 0.9967494640640879 1104 6 0.9671503926062996 1104 7 0.7404995759701123 1104 8 1.0154684103288574 1104 9 1.279111781107062 1104 10-11 1.3462701652417621 1104 12-13 1.1821530671449736 1104 14-15 1.3078333713206263 1104 16-17 1.2653666469574745 1104 18-19 0.9536553960265124 1104 20-21 0.9750917431561064 1104 22-23 0.9888633367659665 1104 24-25 1.0144935192650308 1104 26-27 0.9891179755330199 1104 28-29 0.9494783473617616 1104 30-31 0.9441234879115896 1104 32-33 1.016541267397514 1104 34-35 1.1059968462611565 1104 36-37 1.215597056442327 1104 38-39 1.1147248717905214 1104 40-41 1.4343161811540597 1104 42-43 1.2286522883211504 1104 44-45 1.0567883370835816 1104 46-47 1.1550041147277526 1104 48-49 1.1976714862155404 1104 50-51 1.1288229703823127 1104 52-53 1.0360741133391507 1104 54-55 1.2762133585059985 1104 56-57 1.2305110678971083 1104 58-59 1.1475342384617377 1104 60-61 1.23965429339653 1104 62-63 1.2349389147587253 1104 64-65 1.141031121264053 1104 66-67 1.0193123005906841 1104 68-69 0.9218718944077544 1104 70-71 0.8931073536774505 1104 72-73 0.7475010160606566 1104 74-75 1.0007242274496448 1104 76-77 1.0059924433733691 1104 78-79 1.1463936653083842 1104 80-81 0.8877078959507543 1104 82-83 0.7880676027229114 1104 84-85 0.700950793343214 1104 86-87 0.869985353227829 1104 88-89 0.7917852247107326 1104 90-91 0.7814166314188178 1104 92-93 0.5061279637998268 1104 94-95 0.7260284738712137 1104 96-97 0.6127677571326409 1104 98-99 0.7730416058824154 1104 100-101 0.9647163372250596 1104 102-103 0.7895540568165522 1104 104-105 0.6566105949999432 1104 106-107 0.9574949427217128 1104 108-109 1.1279734987348178 1104 110-111 0.916355745614986 1104 112-113 1.0695399556373886 1104 114-115 0.784938495050902 1104 116-117 0.8738175802579526 1104 118-119 1.1686166500513941 1104 120-121 0.7804893598551743 1104 122-123 1.0982721992402702 1104 124-125 0.8723144430685075 1105 1 -0.12380190915975575 1105 2 -0.17467273812143702 1105 3 -0.07509055040915769 1105 4 -0.03366791330611818 1105 5 -0.14108771424387356 1105 6 0.057796686225955796 1105 7 0.05552782667872691 1105 8 -0.09271021674301494 1105 9 -0.08350155022400685 1105 10-11 -0.05927063403742494 1105 12-13 0.024385530118713916 1105 14-15 0.18411450810661734 1105 16-17 0.28873214167337125 1105 18-19 0.3003929553905422 1105 20-21 0.1429323941425409 1105 22-23 0.26005136822656283 1105 24-25 0.258744828558676 1105 26-27 0.2931720930034132 1105 28-29 0.11830214177919629 1105 30-31 -0.011890815769454832 1105 32-33 -0.024060913819994312 1105 34-35 0.06590148838755994 1105 36-37 0.06969059350613804 1105 38-39 -0.05405668734825042 1105 40-41 8.508234681698923E-4 1105 42-43 0.23072181697543925 1105 44-45 0.11896805288708379 1105 46-47 0.0848677417057715 1105 48-49 -0.14343470266062752 1105 50-51 0.039312336477710375 1105 52-53 0.10845364893880216 1105 54-55 0.16376896828956689 1105 56-57 0.2642218594800809 1105 58-59 -0.0625534232055216 1105 60-61 0.01551401595767743 1105 62-63 0.1417209619496056 1105 64-65 -0.12998327627316186 1105 66-67 0.020856234666105422 1105 68-69 0.23536559665842915 1105 70-71 0.18634689340677113 1105 72-73 0.36201962774011776 1105 74-75 0.2527233639728408 1105 76-77 0.38568055930451806 1105 78-79 0.2114007983776709 1105 80-81 0.2617247525197506 1105 82-83 0.1300818500902423 1105 84-85 0.22760594698577208 1105 86-87 0.2997293135829828 1105 88-89 0.4609140516962569 1105 90-91 0.4902560059612675 1105 92-93 0.5953241795188582 1105 94-95 0.4592399506042675 1105 96-97 0.6021103884843555 1105 98-99 0.5886705361097349 1105 100-101 0.5093214842976863 1105 102-103 0.5089147085538315 1105 104-105 0.6185495697150891 1105 106-107 0.7138185024987109 1105 108-109 0.5325874682878791 1105 110-111 0.5178846627024427 1105 112-113 0.4163549463456242 1105 114-115 0.7962434713052886 1105 116-117 0.6656046956822799 1105 118-119 0.8506272370279113 1105 120-121 0.9376538709583571 1105 122-123 0.5595072526444334 1105 124-125 0.34084681406319817 1106 1 -0.4027307770876156 1106 2 -0.30223177933105205 1106 3 -0.406127591371483 1106 4 -1.2255452206383595 1106 5 -0.4797996708616097 1106 6 -0.4265531980762347 1106 7 -0.46168553421011893 1106 8 -0.5410966297576962 1106 9 -0.2744294289797651 1106 10-11 -0.2796418385310915 1106 12-13 -0.1787391938846028 1106 14-15 -0.1196871805514661 1106 16-17 -0.16214928132134077 1106 18-19 -0.17786733818160627 1106 20-21 0.03948221485509151 1106 22-23 0.17156462489905522 1106 24-25 0.025132407383324562 1106 26-27 0.10142505610375707 1106 28-29 -0.304652833226541 1106 30-31 -0.16672716077855654 1106 32-33 -0.08030682906914421 1106 34-35 -0.11112940557671891 1106 36-37 0.10627450532849991 1106 38-39 0.1683215649966172 1106 40-41 0.17290052936611033 1106 42-43 -0.2900154695135342 1106 44-45 -0.2400925868952939 1106 46-47 -0.2353126938832233 1106 48-49 -0.2519036055627879 1106 50-51 -0.268262778778503 1106 52-53 -0.13310085267743332 1106 54-55 -0.19008527013623677 1106 56-57 -0.06074987845996915 1106 58-59 0.032561656369843206 1106 60-61 0.012809248501440607 1106 62-63 0.02963981450997366 1106 64-65 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-4.164712641193969 2216 108-109 -4.522090551088652 2216 110-111 -4.653221629696148 2216 112-113 -4.949076151591392 2216 114-115 -4.633437034792152 2216 116-117 -4.339465728253902 2216 118-119 -4.503143626560526 2216 120-121 -3.815994148617108 2216 122-123 -3.6182162040965693 2216 124-125 -2.872163212395513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26697.0 3 2788.0 4 1780.0 5 2858.0 6 6153.0 7 5019.0 8 3159.0 9 3142.0 10 2433.0 11 1917.0 12 1674.0 13 1635.0 14 1656.0 15 1884.0 16 1809.0 17 2099.0 18 2273.0 19 2632.0 20 3190.0 21 3750.0 22 4355.0 23 4926.0 24 5335.0 25 5756.0 26 6089.0 27 6656.0 28 7803.0 29 9876.0 30 13008.0 31 17516.0 32 23616.0 33 31805.0 34 44294.0 35 67262.0 36 94982.0 37 23869.0 38 1385.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.35960785367996 18.075606455717754 10.997974747067415 14.566810943534872 2 20.113602806289094 22.94226346544488 34.688006156654644 22.25612757161138 3 31.739757742509145 22.68988580567952 24.396201724658592 21.174154727152747 4 16.86177745066676 21.586493397254205 28.492363722592955 33.05936542948608 5 16.725829793611275 33.20896774323426 26.244047339448723 23.821155123705736 6 32.698626429544404 35.025040093226536 9.325561472925608 22.950772004303452 7 29.014587927941633 40.06066001315195 12.095096648936885 18.829655409969536 8 36.002344089003806 27.812794968260857 11.695170013554561 24.489690929180778 9 36.518498802235825 16.93018969632037 15.27255565521391 31.278755846229895 10-11 30.13735704555977 26.85306755121532 15.33317079826341 27.676404604961498 12-13 32.16192519062456 28.532397787435443 17.64784167593042 21.657835346009577 14-15 35.67324188126142 22.254704383749775 20.715281055195568 21.356772679793238 16-17 29.960656075046465 23.599309300510196 22.016829089900533 24.423205534542802 18-19 28.837486551714107 23.87532068989427 22.27147979234281 25.01571296604881 20-21 37.27674727064102 22.20717405940284 18.231849567082623 22.284229102873514 22-23 33.27301641976993 25.349431760992925 17.33313501072508 24.044416808512064 24-25 27.34313315424653 26.508165150542517 18.431365093282455 27.7173366019285 26-27 24.54845787880889 25.976728944996296 21.070437215794076 28.404375960400735 28-29 26.742750284083282 24.08399917682954 23.311605808720238 25.86164473036694 30-31 28.01336664556693 25.187045805812343 20.706669678784476 26.092917869836253 32-33 24.813848882645953 24.618135782393875 22.91979341643498 27.648221918525195 34-35 23.45973790101614 25.324156812046084 22.57175960615814 28.644345680779633 36-37 24.61936597902403 26.637112124594314 20.442289239358242 28.301232657023416 38-39 25.40959955981328 23.222533623510625 22.39271007844181 28.975156738234286 40-41 23.491387505228335 23.639122936904332 23.608032512978575 29.261457044888754 42-43 23.340520663948304 23.75364305956612 25.448182999577263 27.457653276908317 44-45 23.66730562334063 22.977388985937733 26.424847287863773 26.930458102857855 46-47 21.335523828908727 23.574369859735217 25.79588130168224 29.29422500967382 48-49 20.24411574584585 24.216979844905755 26.924195283649794 28.6147091255986 50-51 21.54365073151965 24.368965046758657 25.631146789298633 28.456237432423066 52-53 21.161506923770308 24.23912338424856 24.79192901541772 29.807440676563413 54-55 19.762437847111165 23.869057870686202 24.76296347658041 31.605540805622223 56-57 20.242238525543527 22.901494139751275 25.759148250872325 31.09711908383287 58-59 21.84046362755909 21.72885124238676 25.186710297640484 31.243974832413667 60-61 21.669130787795552 21.655374952749266 24.773364229908093 31.902130029547088 62-63 21.262942227729525 21.00504827962593 25.136831416090526 32.595178076554014 64-65 20.858739269114285 20.749473241566275 25.39680231907917 32.99498517024027 66-67 21.54557604021629 21.738381618605068 24.260175694642488 32.45586664653615 68-69 20.95995633715234 21.510439267931517 25.001565778348418 32.52803861656772 70-71 20.277867260834164 20.862862741939814 26.20779755353324 32.65147244369279 72-73 20.49845386477887 19.92887218801899 27.53686400161044 32.035809945591694 74-75 20.696450640584718 19.385418938491565 28.18953031243534 31.728600108488376 76-77 20.522320874381965 19.51355625316637 28.299202718072657 31.66492015437901 78-79 20.96963054503973 19.44137202162936 29.249962254866325 30.339035178464584 80-81 22.05475131744938 20.18113251438234 28.900567241363884 28.863548926804388 82-83 21.596685175554846 20.187772950182573 29.062421365184278 29.1531205090783 84-85 21.53189309387507 20.100154583017343 29.12569992997904 29.24225239312855 86-87 22.280601688754682 19.47111782139462 29.138399597382993 29.109880892467704 88-89 23.060617957984764 18.87911642947666 28.958768871454314 29.101496741084258 90-91 24.062588071092105 18.649822627616413 28.434157492361578 28.85343180892991 92-93 24.74095466482934 19.09117143074452 28.00497062810664 28.1629032763195 94-95 24.135084620830895 19.349094622542214 27.7759446651015 28.73987609152539 96-97 23.8991143256897 19.83657692862599 27.035714405544663 29.228594340139647 98-99 23.52428691575406 20.429707071791565 26.910989728723784 29.135016283730586 100-101 23.133048074277703 21.67961367006726 26.889654848471974 28.297683407183072 102-103 22.587752162350167 23.095376026375416 27.36863177530794 26.948240035966474 104-105 21.494387463527183 24.89836818731344 27.232293138021312 26.374951211138065 106-107 20.450928530288373 26.652985220683217 26.667747761853374 26.22833848717504 108-109 20.263798891928346 28.499070642363943 26.42138037008788 24.81575009561983 110-111 19.89462806682428 30.873238302507588 25.24822012914828 23.98391350151985 112-113 19.53310056209557 33.02283499262991 24.39904088110102 23.04502356417351 114-115 19.01982385013281 34.18213337061373 24.269313574723892 22.52872920452957 116-117 18.54789826497541 35.08196464638557 23.948125963188044 22.42201112545098 118-119 18.369228978552147 36.00170562138786 23.625542239870352 22.003523160189634 120-121 18.794546347213508 36.27600645527107 22.986781904215498 21.942665293299914 122-123 19.27775118439948 36.309057804062974 22.740581843234274 21.67260916830328 124-125 19.283053179049475 38.077401286396416 21.83622606225244 20.803319472301666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 0.5 4 0.5 5 3.0 6 3.5 7 3.0 8 6.5 9 8.5 10 7.0 11 6.5 12 10.0 13 15.0 14 19.5 15 25.0 16 26.5 17 33.0 18 55.0 19 85.5 20 119.5 21 179.0 22 249.5 23 325.5 24 427.0 25 610.0 26 778.0 27 901.0 28 1114.0 29 1280.5 30 1402.0 31 1543.0 32 1616.0 33 1727.5 34 1809.5 35 1811.0 36 1782.0 37 1754.5 38 1702.5 39 1616.0 40 1584.0 41 1541.0 42 1510.5 43 1520.0 44 1700.5 45 2104.5 46 2770.0 47 3910.0 48 5747.0 49 9600.0 50 17187.5 51 28633.5 52 41369.0 53 50209.0 54 51471.0 55 46948.5 56 39468.5 57 30450.5 58 22594.0 59 16362.5 60 11758.0 61 8948.0 62 6735.5 63 5002.5 64 3616.0 65 2510.5 66 1761.0 67 1358.0 68 1124.5 69 926.0 70 767.0 71 569.5 72 451.0 73 371.5 74 289.5 75 272.0 76 209.5 77 144.0 78 118.5 79 89.5 80 68.5 81 50.5 82 43.0 83 45.0 84 31.0 85 17.5 86 14.5 87 12.5 88 9.0 89 3.5 90 1.5 91 4.0 92 4.0 93 1.5 94 1.5 95 2.5 96 2.5 97 1.0 98 0.0 99 1.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16082025037856507 2 0.019459473967920946 3 0.00849954035380455 4 0.016104392249313884 5 0.003131409604033255 6 0.0 7 2.2367211457380399E-4 8 2.2367211457380399E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.946884582952159E-4 28-29 0.006933835551787924 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.710163437214119E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0020130490311642355 66-67 1.1183605728690199E-4 68-69 0.0046971144060498835 70-71 0.01588072013474008 72-73 1.1183605728690199E-4 74-75 0.006821999494501021 76-77 3.3550817186070594E-4 78-79 0.0012301966301559218 80-81 0.0015657048020166276 82-83 1.1183605728690199E-4 84-85 0.016551736478461494 86-87 0.0017893769165904319 88-89 0.01755826099404361 90-91 2.2367211457380399E-4 92-93 0.012749310530706827 94-95 0.013308490817141336 96-97 0.00503262257791059 98-99 0.0024603932603118437 100-101 8.946884582952159E-4 102-103 0.0 104-105 7.828524010083138E-4 106-107 0.0012301966301559218 108-109 0.0 110-111 0.0 112-113 0.0013420326874428238 114-115 0.004585278348762982 116-117 0.0 118-119 0.07269343723648629 120-121 0.001453868744729726 122-123 0.0046971144060498835 124-125 0.2479405390050617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 447083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.7596608846665 #Duplication Level Percentage of deduplicated Percentage of total 1 85.10906684941878 56.81852441077609 2 7.658407741015913 10.225454074134545 3 2.6438132343167866 5.2950022489614605 4 1.2825171497800478 3.4248163999234014 5 0.8331215719848236 2.780945681070355 6 0.5489838570581633 2.198998567701553 7 0.3878413667459766 1.812451068470488 8 0.2825490073069084 1.509030072888669 9 0.2073379744690978 1.2457631577663548 >10 1.0065605019411685 11.072030406697726 >50 0.024415583706159757 1.1331683067519966 >100 0.013712862010990128 1.6875834539294987 >500 0.0016723002452426986 0.796232150927837 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 773 0.17289854456555045 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 766 0.17133283976353383 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 708 0.1583598571182532 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 662 0.14807093984785824 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 657 0.14695257927498923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2367211457380396E-4 0.0 2 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 3 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 4 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 5 6.710163437214119E-4 0.0 0.0 2.2367211457380396E-4 0.0 6 6.710163437214119E-4 0.0 0.0 2.2367211457380396E-4 0.0 7 6.710163437214119E-4 0.0 0.0 2.2367211457380396E-4 0.0 8 0.00111836057286902 0.0 0.0 4.473442291476079E-4 0.0 9 0.00111836057286902 0.0 0.0 6.710163437214119E-4 0.0 10-11 0.002907737489459452 0.0 0.0 8.946884582952158E-4 0.0 12-13 0.0039142620050415695 0.0 0.0 0.00111836057286902 0.0 14-15 0.004808950463336785 0.0 0.0 0.0015657048020166278 0.0 16-17 0.005815474978918904 0.0 0.0 0.0022367211457380394 0.0 18-19 0.006486491322640315 0.0 0.0 0.002684065374885648 0.0 20-21 0.00794036006737004 0.0 0.0 0.005927311036205805 0.0 22-23 0.009282392754812865 0.0 0.0 0.023932916259397023 0.0 24-25 0.00961790092667357 0.0 0.0 0.05334579932585225 0.0 26-27 0.009729736983960471 0.0 0.0 0.14169628458250483 0.0 28-29 0.010400753327681884 0.0 0.0 0.4665800310009551 0.0 30-31 0.010848097556829493 0.0 0.0 1.1443065381595812 0.0 32-33 0.011966458129698512 0.0 0.0 2.19880872231778 0.0 34-35 0.013867671103575847 0.0 0.0 3.7534417546630046 0.0 36-37 0.014986031676444866 0.0 0.0 5.85495310714118 0.0 38-39 0.015433375905592473 0.0 0.0 8.524144286407669 0.0 40-41 0.015657048020166278 0.0 0.0 11.585880026751184 0.0 42-43 0.016999080707609103 0.0 0.0 14.089218333061199 0.0 44-45 0.017670097051330512 0.0 0.0 16.321466036507765 0.0 46-47 0.01789376916590432 0.0 0.0 18.817982343323276 0.0 48-49 0.01789376916590432 0.0 0.0 21.456306770778582 0.0 50-51 0.01789376916590432 0.0 0.0 23.931797898824158 0.0 52-53 0.01789376916590432 0.0 0.0 26.13653393217814 0.0 54-55 0.01811744128047812 0.0 0.0 28.262313709087575 0.0 56-57 0.01811744128047812 0.0 0.0 30.46022774294706 0.0 58-59 0.01811744128047812 0.0 0.0 32.5739292256695 0.0 60-61 0.01811744128047812 0.0 0.0 34.739410802915785 0.0 62-63 0.01811744128047812 0.0 0.0 36.78019517628718 0.0 64-65 0.018229277337765023 0.0 0.0 38.69303910012235 0.0 66-67 0.018341113395051924 0.0 0.0 40.73561285041033 0.0 68-69 0.018341113395051924 0.0 0.0 42.907133574750105 0.0 70-71 0.018341113395051924 0.0 0.0 45.19429278232454 0.0 72-73 0.018341113395051924 0.0 0.0 47.42642864971381 0.0 74-75 0.018341113395051924 0.0 0.0 49.391388176244675 0.0 76-77 0.018341113395051924 0.0 0.0 51.319218131756294 0.0 78-79 0.018341113395051924 0.0 0.0 53.28182910108414 0.0 80-81 0.018341113395051924 0.0 0.0 55.19567954943489 0.0 82-83 0.018341113395051924 0.0 0.0 57.09432029399463 2.2367211457380396E-4 84-85 0.018341113395051924 0.0 0.0 59.117434570314686 2.2367211457380396E-4 86-87 0.018341113395051924 0.0 0.0 60.815888772330865 2.2367211457380396E-4 88-89 0.018341113395051924 0.0 0.0 62.26438938631082 2.2367211457380396E-4 90-91 0.018341113395051924 0.0 0.0 63.881650610736706 2.2367211457380396E-4 92-93 0.018341113395051924 0.0 0.0 65.4040748585833 2.2367211457380396E-4 94-95 0.018341113395051924 0.0 0.0 66.76299479067646 2.2367211457380396E-4 96-97 0.018341113395051924 0.0 0.0 68.02517653321642 2.2367211457380396E-4 98-99 0.018341113395051924 0.0 0.0 69.249781360508 2.2367211457380396E-4 100-101 0.018341113395051924 0.0 0.0 70.45526222200351 2.2367211457380396E-4 102-103 0.018341113395051924 0.0 0.0 71.60493689091287 2.2367211457380396E-4 104-105 0.018341113395051924 1.1183605728690198E-4 0.0 72.6198491107915 2.2367211457380396E-4 106-107 0.018341113395051924 2.2367211457380396E-4 0.0 73.58644815392219 2.2367211457380396E-4 108-109 0.018341113395051924 2.2367211457380396E-4 0.0 74.481360284332 2.2367211457380396E-4 110-111 0.018341113395051924 4.473442291476079E-4 0.0 75.38879805315791 2.2367211457380396E-4 112-113 0.018341113395051924 4.473442291476079E-4 0.0 76.24244267842883 2.2367211457380396E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCGC 15 0.0042388937 59.490604 74-75 TTGCACA 35 0.0077876826 51.00906 2 TAGGGTC 50 5.330296E-4 47.59781 5 TGTAGGG 155 0.0 46.072704 2 TGTTAGG 105 6.359187E-9 45.341393 2 GTGTTAG 110 9.451469E-9 43.353172 1 GTTAGAG 55 8.445217E-4 43.353172 1 GTGCGAG 55 8.445217E-4 43.353172 1 GTAATAG 85 3.4319764E-6 42.07808 1 TTAGAGG 60 0.0013065127 39.673717 2 ATAGGGT 75 8.177446E-5 39.669277 4 ACACGGT 90 5.1539973E-6 39.6604 6 ACTAATC 75 8.1882354E-5 39.6604 8 GGAAACA 200 0.0 38.7469 1 TAGGGCA 95 7.464383E-6 37.577217 5 GTTCTAG 65 0.0019200424 36.683456 1 TTCTAGG 65 0.0019359438 36.621895 2 TATATGG 195 1.8189894E-12 36.62189 2 GGGTCAT 130 4.1987732E-8 36.609604 7 GGTCATG 115 6.6536086E-7 36.21167 8 >>END_MODULE