FastQCFastQC Report
Thu 26 May 2016
SRR935948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447083
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT69511.5547448684025114No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT37070.8291525287250914RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT20370.4556200973868387RNA PCR Primer, Index 33 (95% over 24bp)
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7640.17088549553438623No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7360.16462267632631972No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7330.1639516599825983No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7010.15679415231623658No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT6940.15522844751421996No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC6740.15075500522274388No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG5830.13040084279652772RNA PCR Primer, Index 15 (95% over 24bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4610.10311284481852365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC509.076757E-1083.6026151
CTTTACA1000.065.687771
CTATACA657.383278E-964.158462
GTATTGC300.004236059359.4957479
GTCTCTA150.00423744359.495747116-117
ATAGGGT453.1489937E-452.9561844
GGGTATT350.007795355350.9963577
GGTATTG350.007795355350.9963578
CGGAGAT653.4421726E-545.9355051
CACTGTT558.525811E-443.2696387
GCGTCTA355.4532946E-542.49696440-41
CTTGGTA659.1677066E-1041.18936516-17
GTATGGT600.001284543939.810771
GGGTCAT1252.9700459E-838.077287
CTACATT401.197761E-437.18484544-45
TCCAAGG650.001925572836.6619762
TTACGGG1304.1462044E-836.6619762
CCGTCCG650.001938313536.612779
TCAGACG654.2036845E-836.6127720-21
GTGTAGG2051.8189894E-1234.9557951
Produced by FastQC (version 0.11.5)