##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 447083 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.313950653458086 33.0 31.0 34.0 30.0 34.0 2 32.41583777508874 34.0 31.0 34.0 31.0 34.0 3 32.40648380725727 34.0 31.0 34.0 30.0 34.0 4 35.904717915912705 37.0 35.0 37.0 35.0 37.0 5 35.89719805942073 37.0 35.0 37.0 35.0 37.0 6 35.97902179237412 37.0 35.0 37.0 35.0 37.0 7 35.96302252601866 37.0 35.0 37.0 35.0 37.0 8 35.978621419289034 37.0 35.0 37.0 35.0 37.0 9 37.629894225457015 39.0 37.0 39.0 35.0 39.0 10-11 37.55964440607225 39.0 37.0 39.0 35.0 39.0 12-13 37.55423936942358 39.0 37.0 39.0 35.0 39.0 14-15 38.710594006034675 40.0 38.0 41.0 34.0 41.0 16-17 38.626071669018955 40.0 38.0 41.0 34.0 41.0 18-19 38.69751813421669 40.0 38.0 41.0 34.0 41.0 20-21 38.786950074147306 40.0 38.0 41.0 35.0 41.0 22-23 38.63012237101388 40.0 38.0 41.0 34.0 41.0 24-25 38.34291507393482 40.0 38.0 41.0 33.5 41.0 26-27 38.19922139736917 40.0 38.0 41.0 33.0 41.0 28-29 38.14050858565412 40.0 37.0 41.0 33.0 41.0 30-31 37.99013494138672 40.0 37.0 41.0 33.0 41.0 32-33 37.664445975355804 40.0 36.5 41.0 33.0 41.0 34-35 37.51676646170845 40.0 36.0 41.0 33.0 41.0 36-37 37.46517089667914 40.0 36.0 41.0 33.0 41.0 38-39 37.074618135782394 40.0 35.5 41.0 31.5 41.0 40-41 36.87889049684287 39.0 35.0 41.0 31.5 41.0 42-43 36.6218856453947 39.0 35.0 41.0 31.0 41.0 44-45 36.22550846263445 39.0 35.0 41.0 31.0 41.0 46-47 35.74863504092082 38.5 35.0 41.0 30.0 41.0 48-49 35.14225546486894 37.5 35.0 40.0 28.5 41.0 50-51 34.49331444049539 37.0 35.0 40.0 26.5 41.0 52-53 34.01795304227626 36.5 34.0 39.0 26.0 41.0 54-55 33.581984776875885 35.5 34.0 39.0 26.0 41.0 56-57 33.20072223725796 35.0 34.0 38.0 26.0 40.5 58-59 32.858841646853044 35.0 34.0 37.0 25.5 39.5 60-61 32.47068888774568 35.0 33.5 36.5 24.5 39.0 62-63 32.09070239754139 35.0 33.0 36.0 24.0 38.5 64-65 31.77513012125265 35.0 33.0 35.0 23.0 37.0 66-67 31.353445557088953 35.0 33.0 35.0 20.0 36.5 68-69 30.887004202799034 35.0 33.0 35.0 13.0 36.0 70-71 30.407140284913538 35.0 32.5 35.0 4.5 35.5 72-73 30.086426905071317 35.0 32.0 35.0 2.0 35.0 74-75 29.960082356072586 35.0 32.0 35.0 2.0 35.0 76-77 29.820391291997236 35.0 31.5 35.0 2.0 35.0 78-79 29.83637490130468 35.0 32.0 35.0 2.0 35.0 80-81 29.780101233999055 35.0 32.0 35.0 2.0 35.0 82-83 29.662958555793892 35.0 31.5 35.0 2.0 35.0 84-85 29.57842168009072 35.0 31.0 35.0 2.0 35.0 86-87 29.56081085614975 35.0 31.0 35.0 2.0 35.0 88-89 29.485300268630212 35.0 31.0 35.0 2.0 35.0 90-91 29.366310058758664 35.0 31.0 35.0 2.0 35.0 92-93 29.26745033919876 35.0 31.0 35.0 2.0 35.0 94-95 29.23382571021488 35.0 31.0 35.0 2.0 35.0 96-97 29.192327822798003 35.0 31.0 35.0 2.0 35.0 98-99 29.097438283271785 35.0 31.0 35.0 2.0 35.0 100-101 28.968744058709454 35.0 30.5 35.0 2.0 35.0 102-103 28.144295354553854 33.5 28.0 35.0 2.0 35.0 104-105 28.565643292185122 34.0 30.0 35.0 2.0 35.0 106-107 28.372304471429246 35.0 30.0 35.0 2.0 35.0 108-109 27.975694669669835 35.0 29.0 35.0 2.0 35.0 110-111 27.4122780333853 34.0 28.0 35.0 2.0 35.0 112-113 26.681379072789618 34.0 24.5 35.0 2.0 35.0 114-115 25.79538363122731 34.0 18.0 35.0 2.0 35.0 116-117 24.910788377102236 34.0 2.0 35.0 2.0 35.0 118-119 24.156692381504108 33.5 2.0 35.0 2.0 35.0 120-121 23.33476893552204 33.0 2.0 35.0 2.0 35.0 122-123 22.438105452455137 33.0 2.0 35.0 2.0 35.0 124-125 20.20761804855027 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04985022335603162 1101 2 0.0865163663659132 1101 3 0.11267859036158967 1101 4 0.2054269752691198 1101 5 0.14574346507810532 1101 6 0.2542848637856636 1101 7 0.27792453479306545 1101 8 0.10873690960792715 1101 9 0.3018379709721444 1101 10-11 0.07443431043408566 1101 12-13 0.3092884346296785 1101 14-15 0.4299620967493567 1101 16-17 0.2490435023951676 1101 18-19 0.27630266071680154 1101 20-21 0.2349274415081979 1101 22-23 0.2352374508944095 1101 24-25 0.48241905736437474 1101 26-27 0.29785028973807925 1101 28-29 0.23260168519585278 1101 30-31 0.36154371155809883 1101 32-33 0.38681176135061435 1101 34-35 0.5914351166337326 1101 36-37 0.47730524938294394 1101 38-39 0.28070678066078614 1101 40-41 0.0038695714341230314 1101 42-43 -0.15431597929183738 1101 44-45 -0.21254761359437424 1101 46-47 0.03605917546912707 1101 48-49 0.06136446711462895 1101 50-51 0.16012650473467005 1101 52-53 0.16316673267082393 1101 54-55 0.010366453796841313 1101 56-57 -0.0206887462394576 1101 58-59 -0.020340924085658685 1101 60-61 -0.04341924930936614 1101 62-63 -0.06959379616473171 1101 64-65 -0.03800130572154359 1101 66-67 -0.19796968168324725 1101 68-69 0.05431809680975164 1101 70-71 0.30291228458419894 1101 72-73 0.2688340197697947 1101 74-75 0.18903114120058007 1101 76-77 0.24934700571697022 1101 78-79 0.2819572665135226 1101 80-81 0.2990587776084155 1101 82-83 0.10232321318257931 1101 84-85 0.256070979735874 1101 86-87 0.00933935956243559 1101 88-89 0.06335451441064421 1101 90-91 -0.06959821658077203 1101 92-93 -0.11971906357141293 1101 94-95 -0.01087123471665663 1101 96-97 0.08679509047761513 1101 98-99 0.2671957486759595 1101 100-101 0.2915559290067513 1101 102-103 0.25502131747369106 1101 104-105 0.35821932063896966 1101 106-107 0.30336624332787565 1101 108-109 -0.07720045357940464 1101 110-111 0.09700911691926706 1101 112-113 0.10349137342318215 1101 114-115 -0.09537394856765147 1101 116-117 -0.23452038321903856 1101 118-119 -0.6380722128144249 1101 120-121 -0.5197821919846319 1101 122-123 -0.4659888771689431 1101 124-125 -0.6638968863023287 1102 1 0.1177317303259926 1102 2 0.0834794112851256 1102 3 0.11878022376456698 1102 4 0.2205991491996997 1102 5 0.10869765368183693 1102 6 0.23242634808800489 1102 7 0.27456498199415336 1102 8 0.16942812562490417 1102 9 0.2592575924742917 1102 10-11 0.2961244704475945 1102 12-13 0.26734191024205245 1102 14-15 0.23397894239894867 1102 16-17 0.2915344104321349 1102 18-19 0.10730934201798448 1102 20-21 0.2731527508113558 1102 22-23 0.31486112049385895 1102 24-25 0.4592928334462769 1102 26-27 0.4899559707343002 1102 28-29 0.2726693299048719 1102 30-31 0.3703786045337836 1102 32-33 0.42729399453332206 1102 34-35 0.29498293544470755 1102 36-37 0.2335360478879025 1102 38-39 0.5460572654394653 1102 40-41 0.5922676469371098 1102 42-43 0.43289755771490945 1102 44-45 0.3134983717022308 1102 46-47 0.3508729634972312 1102 48-49 0.3105296195042513 1102 50-51 0.18277009222292406 1102 52-53 0.19977149249933746 1102 54-55 0.09150104005266968 1102 56-57 0.09935162888120175 1102 58-59 0.19087867170948414 1102 60-61 0.0889491472035644 1102 62-63 0.11148811593243124 1102 64-65 0.024110096343587628 1102 66-67 0.05676280163870118 1102 68-69 -0.18610919394908265 1102 70-71 -0.06525236122618594 1102 72-73 0.12394731961681416 1102 74-75 0.2054105362794516 1102 76-77 0.12750714019989218 1102 78-79 0.16692345676048603 1102 80-81 0.19460138445000652 1102 82-83 0.06420249610834361 1102 84-85 0.04574104796024869 1102 86-87 0.2651177651736205 1102 88-89 0.3152567068149814 1102 90-91 0.21204219281589332 1102 92-93 0.09863296614790329 1102 94-95 -0.025234394057250142 1102 96-97 0.18768248623192818 1102 98-99 0.31934442589930256 1102 100-101 0.23250920526381336 1102 102-103 0.1647405818500829 1102 104-105 0.38667344749548604 1102 106-107 0.36175643030853877 1102 108-109 0.22985586744565012 1102 110-111 0.17545202846448404 1102 112-113 0.1446805492616221 1102 114-115 -0.12832680141520925 1102 116-117 -0.28546940017809774 1102 118-119 -0.1945948614425035 1102 120-121 0.0015256393270028923 1102 122-123 0.3061475010163939 1102 124-125 0.05691117057567752 1103 1 0.19176804620900612 1103 2 0.2471674777951307 1103 3 0.30326007131289856 1103 4 0.2523508623061943 1103 5 0.2320709430437944 1103 6 0.19421962994586295 1103 7 0.2604478983234628 1103 8 0.22544720183098832 1103 9 0.3208380936690247 1103 10-11 0.29720071602905307 1103 12-13 0.15429416048395694 1103 14-15 0.2414460132336842 1103 16-17 0.5118870536517903 1103 18-19 0.46364289915773327 1103 20-21 0.3974368993924955 1103 22-23 0.4659267517267054 1103 24-25 0.3795198323996516 1103 26-27 0.31110666486333116 1103 28-29 0.2881169098333842 1103 30-31 0.4475708377927319 1103 32-33 0.4456321331221602 1103 34-35 0.43922452789322364 1103 36-37 0.24033540214055904 1103 38-39 0.3671326717468517 1103 40-41 0.2394220141244574 1103 42-43 0.2362371280065787 1103 44-45 0.07007186248996078 1103 46-47 0.07582063229005342 1103 48-49 -0.04976950765070853 1103 50-51 -0.171778773276003 1103 52-53 0.08311980111522388 1103 54-55 0.00817963757631901 1103 56-57 -0.11341052084529224 1103 58-59 -0.08315789101884974 1103 60-61 -0.0978621543624314 1103 62-63 -0.033600155952875355 1103 64-65 0.032631196681268904 1103 66-67 0.05614321989340709 1103 68-69 0.09991788004526825 1103 70-71 0.3085282229085706 1103 72-73 0.31289753585331326 1103 74-75 0.24904908561889272 1103 76-77 0.18693391600510267 1103 78-79 0.11246814563665097 1103 80-81 0.130765951285845 1103 82-83 0.2929762673123548 1103 84-85 0.1883077949339267 1103 86-87 0.41533054583674556 1103 88-89 0.34720095926862626 1103 90-91 0.29302473591508615 1103 92-93 0.22112008082235945 1103 94-95 0.1401149414351579 1103 96-97 0.30095609945493607 1103 98-99 0.48051960708376384 1103 100-101 0.3443079177186412 1103 102-103 0.5138094813149188 1103 104-105 0.2709141019317869 1103 106-107 0.2755012712414171 1103 108-109 0.4266977284618605 1103 110-111 0.4917275275857165 1103 112-113 0.1401879644949382 1103 114-115 0.37149917263641186 1103 116-117 0.16665495061871738 1103 118-119 0.18291432120328466 1103 120-121 0.12018171468394101 1103 122-123 -0.5334721597194019 1103 124-125 -0.517176982823873 1104 1 0.1212797719341765 1104 2 0.17044433188821984 1104 3 0.08983104071887027 1104 4 0.11770838780121551 1104 5 0.25445100732695636 1104 6 0.17069873791776757 1104 7 0.1533936147632886 1104 8 0.1466507255006846 1104 9 0.07373976423765072 1104 10-11 0.16690826731670683 1104 12-13 0.19291861983080594 1104 14-15 0.356089281357967 1104 16-17 0.3452245204189808 1104 18-19 0.412796702578234 1104 20-21 0.2538463672707465 1104 22-23 0.3420154982050221 1104 24-25 0.4300783419826075 1104 26-27 0.3668981017110724 1104 28-29 0.19940019224280547 1104 30-31 0.45438356347822406 1104 32-33 0.4330879880485483 1104 34-35 0.4123605145604188 1104 36-37 0.48917721397342007 1104 38-39 0.833371610677851 1104 40-41 0.7189664766457824 1104 42-43 0.49805461540786666 1104 44-45 0.4162436413876236 1104 46-47 0.4460489962438672 1104 48-49 0.5241090090748131 1104 50-51 0.5322607489651787 1104 52-53 0.4875333164519233 1104 54-55 0.5193175884512797 1104 56-57 0.4188575439140436 1104 58-59 0.5006011860019015 1104 60-61 0.5264806296540812 1104 62-63 0.4023994075565085 1104 64-65 0.4072790281165375 1104 66-67 0.42777033786491714 1104 68-69 0.5695173007031791 1104 70-71 0.7757765463877995 1104 72-73 0.8365473910177919 1104 74-75 0.7454207573779499 1104 76-77 0.8459281027437235 1104 78-79 1.040791922470902 1104 80-81 0.9036693082954841 1104 82-83 0.8999313484599298 1104 84-85 0.9013325756965926 1104 86-87 1.0111650365315583 1104 88-89 0.9679154150441889 1104 90-91 1.1113007963888784 1104 92-93 1.1497885860404082 1104 94-95 1.1301996602955242 1104 96-97 1.0372587263348194 1104 98-99 1.2654586968024262 1104 100-101 1.1307102430231915 1104 102-103 0.9443181912867473 1104 104-105 0.9733192644253386 1104 106-107 0.8972771928318295 1104 108-109 0.8747900660675114 1104 110-111 0.9272211587908288 1104 112-113 0.9297697271234071 1104 114-115 0.716443321678117 1104 116-117 0.933831848360871 1104 118-119 0.7871636565471185 1104 120-121 0.9419596203226064 1104 122-123 1.0892511449113655 1104 124-125 0.6709008712295379 1105 1 0.10968853434665249 1105 2 0.10540669556212379 1105 3 0.1670230516812765 1105 4 0.15592662217886044 1105 5 0.19115909585210034 1105 6 0.15673763191660584 1105 7 0.09008293205347684 1105 8 0.14066739435732956 1105 9 0.13986143938266338 1105 10-11 0.16507572550378313 1105 12-13 0.11216007422609664 1105 14-15 0.21727599883210758 1105 16-17 0.3375095428506043 1105 18-19 0.36163804810035316 1105 20-21 0.45033426920981867 1105 22-23 0.403303580347945 1105 24-25 0.365355123842086 1105 26-27 0.22438453949381199 1105 28-29 0.4219378754969938 1105 30-31 0.47613238558322735 1105 32-33 0.23153185267040044 1105 34-35 0.3227044035848792 1105 36-37 0.23369835976960474 1105 38-39 0.44537018406399653 1105 40-41 0.3362726167167409 1105 42-43 0.3668319510187814 1105 44-45 0.17882271521489201 1105 46-47 0.13081191554633165 1105 48-49 0.10937265106981897 1105 50-51 0.18834591405818912 1105 52-53 0.20975892792490214 1105 54-55 0.15359292492566823 1105 56-57 0.2174609640330516 1105 58-59 0.3077549036449838 1105 60-61 0.38115607500162696 1105 62-63 0.13557099541527862 1105 64-65 -0.07268033215978065 1105 66-67 -0.05225706813812536 1105 68-69 -0.11471298480730141 1105 70-71 -0.21298886948891393 1105 72-73 -0.057564072575399194 1105 74-75 -7.851067558490854E-4 1105 76-77 -0.154189217474773 1105 78-79 -0.04079021106766234 1105 80-81 -0.01642858369482525 1105 82-83 -0.016269165871911184 1105 84-85 -0.12687355123449606 1105 86-87 -0.19641431886423533 1105 88-89 -0.24996230913128414 1105 90-91 -0.2543393965794145 1105 92-93 -0.04393525155086664 1105 94-95 -0.02748340617342393 1105 96-97 -0.13008214052080902 1105 98-99 -0.25339231590569966 1105 100-101 -0.15816498457803263 1105 102-103 -0.30582024657108065 1105 104-105 -0.2031772722818097 1105 106-107 -0.05734569305782955 1105 108-109 -0.1701623488518642 1105 110-111 -0.16161136425086653 1105 112-113 0.09231219704250293 1105 114-115 0.3781551644502521 1105 116-117 0.44173453827884046 1105 118-119 0.40457069200598283 1105 120-121 0.375819173942638 1105 122-123 0.43402007100901585 1105 124-125 0.628724252679806 1106 1 -0.029148835162118303 1106 2 0.11548830226023199 1106 3 0.17353657331058514 1106 4 0.18020549805542174 1106 5 0.19146379618594267 1106 6 0.21233881892312212 1106 7 0.13069639539228461 1106 8 0.17271392077352488 1106 9 0.274662633454696 1106 10-11 0.24881753392234174 1106 12-13 0.27463600731566373 1106 14-15 0.3721123438412093 1106 16-17 0.45898340927703885 1106 18-19 0.4559648811599004 1106 20-21 0.1710123820107654 1106 22-23 0.2730684753367285 1106 24-25 0.3470201124489378 1106 26-27 0.3615902166351077 1106 28-29 0.39218322550493667 1106 30-31 0.25163668671547157 1106 32-33 0.4605914326255345 1106 34-35 0.20141600692686268 1106 36-37 -0.1092197862392652 1106 38-39 0.013932175126114998 1106 40-41 0.057679360750796604 1106 42-43 0.030440574479825955 1106 44-45 0.3088745584311141 1106 46-47 0.23252221176804966 1106 48-49 0.016156428647406074 1106 50-51 -0.27572373425580565 1106 52-53 -0.17161769935152194 1106 54-55 -0.19834037661284043 1106 56-57 -0.13651692090503786 1106 58-59 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54-55 -1.7817374110800586 2216 56-57 -1.8334024254670211 2216 58-59 -1.6274958572916134 2216 60-61 -2.1031004949150187 2216 62-63 -1.7833320622567541 2216 64-65 -1.7549544881667067 2216 66-67 -1.8541400132114347 2216 68-69 -1.737328219454291 2216 70-71 -1.615096614132515 2216 72-73 -1.6172404009817107 2216 74-75 -1.7084685770246182 2216 76-77 -1.926340927344068 2216 78-79 -1.982802366159472 2216 80-81 -1.8153498441639222 2216 82-83 -1.934908631127449 2216 84-85 -2.002803584854963 2216 86-87 -2.3823638483766345 2216 88-89 -2.231834113694916 2216 90-91 -2.288870003140385 2216 92-93 -2.518834290681909 2216 94-95 -2.6761147149843687 2216 96-97 -2.6048667904899716 2216 98-99 -2.674722413186224 2216 100-101 -2.5113788226825555 2216 102-103 -2.383736672854724 2216 104-105 -2.733525214271584 2216 106-107 -2.733577117252633 2216 108-109 -2.6545556884185757 2216 110-111 -2.5717686244812334 2216 112-113 -2.033841641459894 2216 114-115 -1.9090188264384018 2216 116-117 -1.7798472151578046 2216 118-119 -1.7224932863496 2216 120-121 -2.0948872537335674 2216 122-123 -2.1505607673143388 2216 124-125 -2.3393835516451027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 24.0 5 80.0 6 218.0 7 531.0 8 833.0 9 1207.0 10 1660.0 11 2910.0 12 4231.0 13 4379.0 14 5653.0 15 7408.0 16 3781.0 17 2010.0 18 2252.0 19 2874.0 20 4267.0 21 5881.0 22 5963.0 23 2306.0 24 2949.0 25 3835.0 26 4818.0 27 5935.0 28 7590.0 29 9379.0 30 12245.0 31 16771.0 32 22878.0 33 30514.0 34 40704.0 35 60175.0 36 124516.0 37 43944.0 38 2352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.48840180192295 19.63087473946077 11.092583876823776 16.78813958179251 2 19.118459518521252 20.60856981173434 36.933352121875004 23.3396185478694 3 29.62134547723801 21.867304281307945 27.885202523916142 20.626147717537908 4 16.202519740053834 21.933069684741373 27.278223910210187 34.5861866649946 5 15.708525938488663 33.01009974448586 28.37952128016611 22.901853036859368 6 32.4355639260878 34.47394261761554 9.659986892462271 23.43050656383438 7 27.98451294278691 39.29740115370077 12.093280218661858 20.624805684850465 8 35.94075373029169 27.521064321389986 12.961575367437367 23.576606580880956 9 33.98384639988548 14.433785225562143 16.96955598848536 34.61281238606701 10-11 29.155436462580774 24.77750216402771 18.265847728497842 27.80121364489368 12-13 32.427423990623666 25.403224904547926 19.86834659336186 22.30100451146655 14-15 35.08543156416146 19.62711621779401 22.445608533538515 22.841843684506006 16-17 30.44971515356209 21.482811916355576 24.180968634459372 23.88650429562296 18-19 29.301606189454755 21.443557460247874 24.448480483489643 24.80635586680773 20-21 35.141796937034066 19.396957611897566 22.234126549208984 23.227118901859388 22-23 31.329417580180863 22.947752430756704 21.559866959826252 24.16296302923618 24-25 26.90238725247885 23.982124124603263 22.749243429072454 26.36624519384544 26-27 24.494556939091847 23.815935743474924 25.16881652847458 26.52069078895865 28-29 26.158341963349084 21.24627865519378 27.599461397548108 24.995917983909028 30-31 28.39853002686302 21.680538065638817 25.18279603563544 24.738135871862717 32-33 24.54890926293328 20.958300807680004 27.644307656520155 26.84848227286656 34-35 23.183950183746642 20.886949403130963 27.610756839334083 28.31834357378831 36-37 24.212394566556995 22.956699315339655 25.386337659897602 27.444568458205747 38-39 25.3017896005887 18.180628652845222 27.871223016755277 28.646358729810796 40-41 23.217836509104572 18.518038037679805 29.104103712286083 29.160021740929537 42-43 24.356439408342524 18.48638843346761 30.50250177260151 26.654670385588357 44-45 23.74793942064449 18.678299107771934 30.704925036290803 26.868836435292774 46-47 22.26208556353071 18.38897922757072 29.409639820793902 29.939295388104668 48-49 21.058282242894496 19.388234399429187 30.083899410176635 29.469583947499682 50-51 21.44691254196648 19.901338230261494 29.12401053048315 29.527738697288868 52-53 20.815486162524632 20.416119601953106 28.69880984067835 30.06958439484391 54-55 19.015708492606517 20.578281885019113 27.234764014735518 33.17124560763885 56-57 19.919008327312827 20.776343542474216 26.529078493255163 32.77556963695779 58-59 19.930639277270664 20.98603614988716 26.583878161325746 32.49944641151643 60-61 20.79211242655167 22.64422937128005 23.73496643799921 32.82869176416907 62-63 19.24474873793009 23.936606849287493 23.594947694276005 33.22369671850641 64-65 18.624170456045075 24.19908607573985 23.30428578138735 33.87245768682772 66-67 19.69824395022848 25.444716081801367 22.061451676758008 32.79558829121215 68-69 20.065178054186806 25.582833612550687 21.82592494011179 32.526063393150714 70-71 20.17522473455712 25.69041989966069 21.90286814752518 32.23148721825701 72-73 20.583873687883457 25.222609672029577 22.071628757971116 32.12188788211584 74-75 20.937051956795493 25.243858522914092 22.085943773303836 31.73314574698658 76-77 20.97004359369513 25.091090468660184 22.308497527304773 31.630368410339916 78-79 21.036026867494403 25.073308535551565 22.92549705535661 30.96516754159742 80-81 20.553006936072276 26.010047351386657 22.503427775155842 30.93351793738523 82-83 19.89574642739715 25.85247034666941 23.073903503376332 31.177879722557112 84-85 20.280238792349518 24.515470281804497 23.38793915223795 31.81635177360803 86-87 20.746706987293187 24.016234122075765 23.70063276841213 31.53642612221892 88-89 20.757331412735443 24.110176410196765 23.683410015589946 31.449082161477847 90-91 20.57716352444624 23.462533802448316 23.8648081005093 32.09549457259614 92-93 20.436585600436608 22.961508265802994 24.270101972117033 32.33180416164336 94-95 20.24076066412724 22.736046774312598 24.70961767725456 32.3135748843056 96-97 20.15889667019323 23.147379793013826 24.5939791940199 32.09974434277304 98-99 20.499437464631846 23.668312147856216 24.523746150043728 31.30850423746821 100-101 20.760015478110326 24.679086433615236 24.03893684170501 30.52196124656943 102-103 20.21168328923265 26.288742806145613 23.683074507418088 29.81649939720365 104-105 19.766575781230777 28.400095731665036 23.329001550047753 28.50432693705643 106-107 19.503537374491987 30.954766788269737 22.29317598745647 27.24851984978181 108-109 19.042884654527235 32.44632428430515 21.769224059067334 26.74156700210028 110-111 18.56022564345739 33.85669582644601 21.404590260738082 26.178488269358525 112-113 18.400936744053922 35.15440731590843 20.99633063844207 25.448325301595574 114-115 18.21001116128845 36.00365481870176 20.816697645393116 24.96963637461668 116-117 17.793636893358073 36.380341407166576 20.915578729101032 24.910442970374323 118-119 17.767724835179582 36.85915752861641 20.933730729012307 24.439386907191707 120-121 18.12937700327234 37.04060338059546 20.77632464810627 24.05369496802593 122-123 18.176144942123802 36.83162780294134 20.729295979421796 24.262931275513058 124-125 17.124779482750714 38.61582846806373 20.498567546796643 23.760824502388914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.5 10 5.0 11 5.0 12 4.5 13 4.0 14 3.5 15 8.5 16 12.0 17 17.0 18 24.5 19 38.0 20 50.5 21 75.5 22 110.5 23 171.0 24 263.0 25 388.5 26 569.0 27 778.5 28 1045.5 29 1339.0 30 1623.0 31 1850.5 32 2015.0 33 2098.0 34 2145.5 35 2216.5 36 2196.5 37 2113.5 38 2066.5 39 2061.5 40 2076.0 41 2153.0 42 2337.5 43 2547.5 44 2865.0 45 3203.5 46 3692.5 47 4521.0 48 5825.0 49 8435.0 50 14343.5 51 24921.0 52 37831.5 53 48247.5 54 51561.5 55 48573.0 56 42440.5 57 33468.5 58 24035.0 59 16677.5 60 11690.0 61 8481.5 62 6173.5 63 4467.0 64 3085.5 65 2045.5 66 1325.0 67 968.5 68 833.5 69 693.0 70 563.0 71 384.0 72 330.5 73 288.5 74 195.5 75 178.0 76 124.5 77 73.5 78 51.5 79 30.5 80 16.0 81 12.0 82 8.0 83 3.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19973919831440695 2 4.4734422914760797E-4 3 0.0 4 0.1183225486095423 5 0.03153776815490636 6 0.0026840653748856475 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0013420326874428238 112-113 5.5918028643451E-4 114-115 4.4734422914760797E-4 116-117 0.004808950463336786 118-119 0.0012301966301559218 120-121 4.4734422914760797E-4 122-123 0.0017893769165904319 124-125 0.027399834035290987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 447083.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.13479281232389 #Duplication Level Percentage of deduplicated Percentage of total 1 82.42977283687199 49.568973111122205 2 8.938236961512224 10.749980555759862 3 3.0909788205246516 5.576261128785935 4 1.5779264642744255 3.7955312400890153 5 0.9456683631522818 2.843378554366596 6 0.6521019471464751 2.352840929049976 7 0.46869043887000716 1.9729221704195528 8 0.32294249506411604 1.5536064024780443 9 0.2517443729055648 1.3624736135710087 >10 1.2666397972351064 12.663094765396865 >50 0.03228780344956187 1.3450742497590973 >100 0.019298457220449903 2.292451084444982 >500 0.00259786924121441 1.090208678690979 >1k 7.422483546326886E-4 1.2819157933267111 >5k 3.711241773163443E-4 1.5512877227392006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 6951 1.5547448684025114 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 3707 0.8291525287250914 RNA PCR Primer, Index 33 (95% over 22bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 2037 0.4556200973868387 RNA PCR Primer, Index 33 (95% over 24bp) GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 764 0.17088549553438623 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 736 0.16462267632631972 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 733 0.1639516599825983 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 701 0.15679415231623658 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 694 0.15522844751421996 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 674 0.15075500522274388 No Hit CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 583 0.13040084279652772 RNA PCR Primer, Index 15 (95% over 24bp) TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 461 0.10311284481852365 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2367211457380396E-4 0.0 2 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 3 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 4 2.2367211457380396E-4 0.0 0.0 2.2367211457380396E-4 0.0 5 8.946884582952158E-4 0.0 0.0 2.2367211457380396E-4 0.0 6 8.946884582952158E-4 0.0 0.0 2.2367211457380396E-4 0.0 7 0.0015657048020166278 0.0 0.0 2.2367211457380396E-4 0.0 8 0.0017893769165904317 0.0 0.0 4.473442291476079E-4 0.0 9 0.0020130490311642355 0.0 0.0 6.710163437214119E-4 0.0 10-11 0.002907737489459452 0.0 0.0 8.946884582952158E-4 0.0 12-13 0.002907737489459452 0.0 0.0 8.946884582952158E-4 0.0 14-15 0.0032432456613201576 0.0 0.0 0.001342032687442824 0.0 16-17 0.0036905898904677657 1.1183605728690198E-4 0.0 0.0020130490311642355 0.0 18-19 0.004137934119615373 2.2367211457380396E-4 0.0 0.0024603932603118437 0.0 20-21 0.0052562946924843935 2.2367211457380396E-4 0.0 0.005703638921632 0.0 22-23 0.006486491322640315 2.2367211457380396E-4 0.0 0.02382108020211012 0.0 24-25 0.006933835551787923 2.2367211457380396E-4 0.0 0.05301029115399154 0.0 26-27 0.007269343723648629 2.2367211457380396E-4 0.0 0.14236730092622624 0.0 28-29 0.008052196124656942 2.2367211457380396E-4 0.0 0.4664681949436682 0.0 30-31 0.00838770429651765 2.2367211457380396E-4 0.0 1.1485563083364834 0.0 32-33 0.009170556697525964 2.2367211457380396E-4 0.0 2.2050715415258466 0.0 34-35 0.010288917270394982 2.2367211457380396E-4 0.0 3.7625004753032436 0.0 36-37 0.010624425442255689 2.2367211457380396E-4 0.0 5.868597106130181 0.0 38-39 0.010959933614116395 2.2367211457380396E-4 0.0 8.54617598969319 0.0 40-41 0.0112954417859771 2.2367211457380396E-4 0.0 11.618871663650822 0.0 42-43 0.01185462207241161 2.2367211457380396E-4 0.0 14.133281739632238 0.0 44-45 0.01185462207241161 2.2367211457380396E-4 0.0 16.37559468823462 0.0 46-47 0.011966458129698512 2.2367211457380396E-4 0.0 18.880498699346653 0.0 48-49 0.012078294186985415 2.2367211457380396E-4 0.0 21.538058928655303 0.0 50-51 0.012078294186985415 2.2367211457380396E-4 0.0 24.025069170601434 0.0 52-53 0.012078294186985415 2.2367211457380396E-4 0.0 26.240094121225813 0.0 54-55 0.012078294186985415 2.2367211457380396E-4 0.0 28.383208487014716 0.0 56-57 0.012078294186985415 2.2367211457380396E-4 0.0 30.606844814050184 0.0 58-59 0.012190130244272316 2.2367211457380396E-4 0.0 32.742242491886294 0.0 60-61 0.012525638416133023 2.2367211457380396E-4 0.0 34.94060386997492 0.0 62-63 0.012525638416133023 2.2367211457380396E-4 0.0 37.03204997729728 0.0 64-65 0.012525638416133023 2.2367211457380396E-4 0.0 38.97956307889139 0.0 66-67 0.012525638416133023 2.2367211457380396E-4 0.0 41.05289174493326 0.0 68-69 0.012525638416133023 2.2367211457380396E-4 0.0 43.26825220372951 0.0 70-71 0.012525638416133023 2.2367211457380396E-4 0.0 45.591981802036756 0.0 72-73 0.012525638416133023 2.2367211457380396E-4 0.0 47.85710930632567 0.0 74-75 0.012637474473419924 2.2367211457380396E-4 0.0 49.85136987986571 0.0 76-77 0.012749310530706826 2.2367211457380396E-4 0.0 51.79865930934524 0.0 78-79 0.012749310530706826 2.2367211457380396E-4 0.0 53.78889378482295 0.0 80-81 0.012749310530706826 2.2367211457380396E-4 0.0 55.73629505035977 0.0 82-83 0.012749310530706826 2.2367211457380396E-4 0.0 57.66982864479303 0.0 84-85 0.012749310530706826 2.2367211457380396E-4 0.0 59.71777052583077 0.0 86-87 0.012749310530706826 2.2367211457380396E-4 0.0 61.445749446970694 0.0 88-89 0.012749310530706826 2.2367211457380396E-4 0.0 62.919413173840205 0.0 90-91 0.012749310530706826 2.2367211457380396E-4 0.0 64.5630677077858 0.0 92-93 0.012749310530706826 2.2367211457380396E-4 0.0 66.11613503532901 0.0 94-95 0.012749310530706826 2.2367211457380396E-4 0.0 67.50390867020218 0.0 96-97 0.012749310530706826 2.2367211457380396E-4 0.0 68.78644457516837 0.0 98-99 0.012749310530706826 2.2367211457380396E-4 0.0 70.02983786008414 0.0 100-101 0.012749310530706826 2.2367211457380396E-4 0.0 71.25254147440185 0.0 102-103 0.012749310530706826 2.2367211457380396E-4 0.0 72.42715558408618 0.0 104-105 0.012749310530706826 2.2367211457380396E-4 0.0 73.46387583513575 0.0 106-107 0.012749310530706826 2.2367211457380396E-4 0.0 74.44948700800524 0.0 108-109 0.012749310530706826 2.2367211457380396E-4 0.0 75.3641941205548 0.0 110-111 0.012749310530706826 2.2367211457380396E-4 0.0 76.29377542872354 0.0 112-113 0.012749310530706826 2.2367211457380396E-4 0.0 77.1675505443061 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 50 9.076757E-10 83.602615 1 CTTTACA 100 0.0 65.68777 1 CTATACA 65 7.383278E-9 64.15846 2 GTATTGC 30 0.0042360593 59.495747 9 GTCTCTA 15 0.004237443 59.495747 116-117 ATAGGGT 45 3.1489937E-4 52.956184 4 GGGTATT 35 0.0077953553 50.996357 7 GGTATTG 35 0.0077953553 50.996357 8 CGGAGAT 65 3.4421726E-5 45.935505 1 CACTGTT 55 8.525811E-4 43.269638 7 GCGTCTA 35 5.4532946E-5 42.496964 40-41 CTTGGTA 65 9.1677066E-10 41.189365 16-17 GTATGGT 60 0.0012845439 39.81077 1 GGGTCAT 125 2.9700459E-8 38.07728 7 CTACATT 40 1.197761E-4 37.184845 44-45 TCCAAGG 65 0.0019255728 36.661976 2 TTACGGG 130 4.1462044E-8 36.661976 2 CCGTCCG 65 0.0019383135 36.61277 9 TCAGACG 65 4.2036845E-8 36.61277 20-21 GTGTAGG 205 1.8189894E-12 34.955795 1 >>END_MODULE