##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935947_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442521 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.788343462590838 31.0 31.0 34.0 28.0 34.0 2 30.932230449331414 31.0 31.0 34.0 28.0 34.0 3 31.192826308941083 33.0 31.0 34.0 28.0 34.0 4 34.465675023101916 37.0 35.0 37.0 32.0 37.0 5 34.62759918226494 37.0 35.0 37.0 32.0 37.0 6 34.71370607429632 37.0 35.0 37.0 32.0 37.0 7 34.689959452929976 37.0 35.0 37.0 32.0 37.0 8 34.657108631347484 37.0 35.0 37.0 32.0 37.0 9 36.26040938052201 39.0 37.0 39.0 32.0 39.0 10-11 36.01103762094278 39.0 37.0 39.0 31.0 39.0 12-13 35.82633701693078 39.0 37.0 39.0 30.0 39.0 14-15 37.24439470898517 40.0 38.0 41.0 31.0 41.0 16-17 37.378722736098815 40.0 38.0 41.0 31.0 41.0 18-19 37.40905401030557 40.0 38.0 41.0 31.0 41.0 20-21 37.49598203423035 40.0 38.0 41.0 31.0 41.0 22-23 37.39469754686414 40.0 38.0 41.0 31.0 41.0 24-25 37.34383936178399 40.0 37.0 41.0 31.0 41.0 26-27 37.312955236006964 40.0 37.0 41.0 30.5 41.0 28-29 37.21953337247777 40.0 37.0 41.0 30.0 41.0 30-31 37.151060539153335 40.0 37.0 41.0 30.0 41.0 32-33 37.06063273948871 40.0 36.5 41.0 30.0 41.0 34-35 36.83671606860489 40.0 36.0 41.0 30.0 41.0 36-37 36.818442851091945 40.0 36.0 41.0 30.0 41.0 38-39 36.916677816128846 40.0 36.0 41.0 30.0 41.0 40-41 36.87132630998093 40.0 36.0 41.0 30.0 41.0 42-43 36.71881622520573 40.0 35.0 41.0 30.0 41.0 44-45 36.539174473023266 40.0 35.0 41.0 30.0 41.0 46-47 36.37841355515795 40.0 35.0 41.0 30.0 41.0 48-49 36.21044026395457 39.5 35.0 41.0 30.0 41.0 50-51 35.97423295744048 39.0 35.0 41.0 29.0 41.0 52-53 35.72879320301057 39.0 35.0 41.0 29.0 41.0 54-55 35.46708262825983 38.5 35.0 41.0 29.0 41.0 56-57 35.20292529536832 38.0 35.0 40.0 28.0 41.0 58-59 34.85155328761245 37.0 34.0 40.0 28.0 41.0 60-61 34.54356470373742 37.0 34.0 39.5 28.0 41.0 62-63 34.133843805393475 36.0 34.0 39.0 26.5 41.0 64-65 33.7154457370118 35.5 34.0 39.0 26.0 41.0 66-67 33.4015979663381 35.0 33.0 37.5 26.5 40.0 68-69 33.20523063442404 35.0 33.5 37.0 26.5 39.5 70-71 33.002599268918786 35.0 33.5 36.5 27.0 39.0 72-73 32.73484476135876 35.0 33.5 36.0 26.5 39.0 74-75 32.422579636622274 35.0 33.0 35.5 26.0 37.5 76-77 32.20403550450912 35.0 33.0 35.0 26.0 37.0 78-79 31.999322366884087 35.0 33.0 35.0 25.5 37.0 80-81 31.802569945255563 35.0 33.0 35.0 25.0 36.0 82-83 31.669035320802955 35.0 33.0 35.0 25.0 36.0 84-85 31.459801625071663 35.0 33.0 35.0 24.5 35.5 86-87 31.310487680941907 35.0 33.0 35.0 24.0 35.0 88-89 31.21546168132041 35.0 33.0 35.0 24.0 35.0 90-91 31.152389115999004 35.0 33.0 35.0 23.0 35.0 92-93 31.049023549743815 35.0 32.5 35.0 22.5 35.5 94-95 30.87613282579595 35.0 32.0 35.0 20.0 35.0 96-97 30.759752890945784 35.0 32.0 35.0 19.5 35.0 98-99 30.607356496023282 35.0 32.0 35.0 19.0 35.0 100-101 30.50677459808211 35.0 32.0 35.0 18.0 35.0 102-103 29.818018940452166 34.0 30.5 35.0 17.0 35.0 104-105 30.37083446272186 35.0 31.5 35.0 18.0 35.0 106-107 30.424986533991532 35.0 32.0 35.0 17.0 35.0 108-109 30.34074685914451 35.0 32.0 35.0 15.0 35.0 110-111 30.24491844486146 35.0 32.0 35.0 12.5 35.0 112-113 30.117308170903577 35.0 31.5 35.0 7.5 35.0 114-115 29.927805210461408 35.0 31.0 35.0 2.0 35.0 116-117 29.788296669511226 35.0 31.0 35.0 2.0 35.0 118-119 29.585558893076772 35.0 31.0 35.0 2.0 35.0 120-121 29.382189929990624 35.0 31.0 35.0 2.0 35.0 122-123 29.017956411033182 35.0 31.0 35.0 2.0 35.0 124-125 26.21968103064011 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2745202782292502 1101 2 0.5392846321573153 1101 3 0.5195186001252878 1101 4 0.42521832785629954 1101 5 0.4356400550619881 1101 6 0.5084911805162875 1101 7 0.41150114623730616 1101 8 0.4164034492761317 1101 9 0.4236436232209613 1101 10-11 0.5228502377150761 1101 12-13 0.4007249033082445 1101 14-15 0.5101130601722446 1101 16-17 0.4745774998767587 1101 18-19 0.4891334727146699 1101 20-21 0.4731378960141299 1101 22-23 0.5092370963472774 1101 24-25 0.5291935235311058 1101 26-27 0.5181476016090159 1101 28-29 0.4688953272898999 1101 30-31 0.33904895149948544 1101 32-33 0.39878222262044716 1101 34-35 0.0318712572903479 1101 36-37 0.12431055437374994 1101 38-39 0.28117897149602555 1101 40-41 0.2815309393618364 1101 42-43 0.2627369966339046 1101 44-45 0.2909039048865125 1101 46-47 0.3123103456235228 1101 48-49 0.22777780608202391 1101 50-51 0.3504537317151559 1101 52-53 0.34173209055527565 1101 54-55 0.2145835627942887 1101 56-57 0.04488477599196017 1101 58-59 0.04846294386371852 1101 60-61 0.2601740167714013 1101 62-63 0.3125175096627544 1101 64-65 0.26419103485593354 1101 66-67 0.22906730496204375 1101 68-69 0.15563934103630572 1101 70-71 0.02498095374176046 1101 72-73 0.1263347818135614 1101 74-75 0.14429188050728925 1101 76-77 0.38984176690732397 1101 78-79 0.4500662144016303 1101 80-81 0.3605770058983424 1101 82-83 0.33231577108906407 1101 84-85 0.1727776904223255 1101 86-87 0.05731820286286293 1101 88-89 0.1995241416102118 1101 90-91 0.14928211818663328 1101 92-93 -0.12530832479822607 1101 94-95 -0.1446829514183392 1101 96-97 -0.142716363690365 1101 98-99 -0.20683215068496708 1101 100-101 0.03819219432287824 1101 102-103 0.044456913987005464 1101 104-105 -0.06127079344677355 1101 106-107 0.038263952762058295 1101 108-109 0.10301030299923042 1101 110-111 0.0663136551276402 1101 112-113 0.1179961007276411 1101 114-115 0.01526533302743971 1101 116-117 -0.01351911221957991 1101 118-119 0.009881230095651006 1101 120-121 -0.2970324958664108 1101 122-123 -0.34409021853425514 1101 124-125 -0.23040067873016667 1102 1 0.21669750765735074 1102 2 0.3720853571770668 1102 3 0.35506718668827375 1102 4 0.2885030463533198 1102 5 0.3849727281749722 1102 6 0.4179317201852939 1102 7 0.3149622597172623 1102 8 0.3840182657211244 1102 9 0.5345316043068706 1102 10-11 0.43355352521506063 1102 12-13 0.4385297776294905 1102 14-15 0.4710836452161118 1102 16-17 0.49027798296854286 1102 18-19 0.4137697784150305 1102 20-21 0.3286191767804709 1102 22-23 0.4312669071299524 1102 24-25 0.3936099391764927 1102 26-27 0.48416268275346397 1102 28-29 0.378627005793156 1102 30-31 0.3385125224662957 1102 32-33 0.42273049313350697 1102 34-35 0.359451733107484 1102 36-37 0.29272825683750625 1102 38-39 0.2745079950576397 1102 40-41 0.25037755580896714 1102 42-43 0.06632029672697115 1102 44-45 0.21090071076491057 1102 46-47 0.1769487450782421 1102 48-49 0.1297675604403139 1102 50-51 0.042089565174570964 1102 52-53 0.10104012766822024 1102 54-55 0.2383774762351294 1102 56-57 0.3183611876332293 1102 58-59 0.12336165658686582 1102 60-61 0.09254261840330713 1102 62-63 0.226952876077398 1102 64-65 0.1152754314955331 1102 66-67 0.11164933199134452 1102 68-69 0.2504191682480794 1102 70-71 0.1591516359847418 1102 72-73 0.14704078966590828 1102 74-75 0.2704696173269525 1102 76-77 0.2782523017010874 1102 78-79 0.35595119240193185 1102 80-81 0.2841431280360496 1102 82-83 0.2611556325039217 1102 84-85 0.3607781091731965 1102 86-87 0.3043430085028547 1102 88-89 0.33112271541199334 1102 90-91 0.44307558842399075 1102 92-93 0.5054099552055042 1102 94-95 0.354967070665424 1102 96-97 0.3316741169103139 1102 98-99 0.38367955065091053 1102 100-101 0.4952178863698222 1102 102-103 0.5197020895643547 1102 104-105 0.34189001147186815 1102 106-107 0.08680428504240467 1102 108-109 0.261848315185798 1102 110-111 0.25710893027294546 1102 112-113 0.1886320365823444 1102 114-115 0.28606724094198555 1102 116-117 0.24720526565264578 1102 118-119 0.07284859962189927 1102 120-121 0.14111158194177875 1102 122-123 -0.037937484503718366 1102 124-125 -0.1928204627219614 1103 1 0.2856518164610158 1103 2 0.356755138327447 1103 3 0.42880628008648003 1103 4 0.4720811744029021 1103 5 0.5373253670676235 1103 6 0.3823407882012617 1103 7 0.5243602423446703 1103 8 0.6166004144322343 1103 9 0.5836272417636152 1103 10-11 0.7080286718445734 1103 12-13 0.6426348192091424 1103 14-15 0.6778147506531553 1103 16-17 0.6887954965179617 1103 18-19 0.6753535160440194 1103 20-21 0.6002591978230924 1103 22-23 0.6905166094362869 1103 24-25 0.7246773406293556 1103 26-27 0.5937412430588793 1103 28-29 0.5906958860288043 1103 30-31 0.6038224564802803 1103 32-33 0.5766557245694699 1103 34-35 0.7159088044255597 1103 36-37 0.7768170266895353 1103 38-39 0.7921238823845798 1103 40-41 0.8529085672413288 1103 42-43 0.7200417051597654 1103 44-45 0.6158649765598554 1103 46-47 0.7432832033975885 1103 48-49 0.6549609608147264 1103 50-51 0.5240805297118101 1103 52-53 0.6781959671058857 1103 54-55 0.7103696615174258 1103 56-57 0.6157837830152104 1103 58-59 0.7071927298362013 1103 60-61 0.7092194092131692 1103 62-63 0.4335764048785862 1103 64-65 0.43022972909076174 1103 66-67 0.4552565386269407 1103 68-69 0.6180872357257954 1103 70-71 0.620660642573597 1103 72-73 0.5581318068096977 1103 74-75 0.6162426108886763 1103 76-77 0.5934340574833641 1103 78-79 0.5814861577325274 1103 80-81 0.6809143913561257 1103 82-83 0.695955297517834 1103 84-85 0.6023718418046897 1103 86-87 0.7258526339923996 1103 88-89 0.6752302813267477 1103 90-91 0.5118180333887352 1103 92-93 0.6049294299878589 1103 94-95 0.6955722434568941 1103 96-97 0.589555902492414 1103 98-99 0.6871049954126853 1103 100-101 0.694785581800506 1103 102-103 0.8562802656152826 1103 104-105 0.725449937993254 1103 106-107 0.6693194633381907 1103 108-109 0.6629348270249551 1103 110-111 0.6359549777513998 1103 112-113 0.7259044778848569 1103 114-115 0.7156533986188727 1103 116-117 0.7796229914557635 1103 118-119 0.7101374637222726 1103 120-121 0.6557940261901578 1103 122-123 0.6836315213395316 1103 124-125 0.5765368137836155 1104 1 -0.04793684183457003 1104 2 0.04391610846808902 1104 3 0.10805735826914997 1104 4 0.14902278648325762 1104 5 0.11940551178317094 1104 6 0.17818492691525734 1104 7 0.12234190322884331 1104 8 0.08125298169646555 1104 9 0.14679214070533675 1104 10-11 0.23696848440212648 1104 12-13 0.2097108537580965 1104 14-15 0.3758312714030225 1104 16-17 0.31153402161588417 1104 18-19 0.24577044474852983 1104 20-21 0.35085805734019004 1104 22-23 0.4086365556390348 1104 24-25 0.353221799912788 1104 26-27 0.42815735208546357 1104 28-29 0.4624267725764284 1104 30-31 0.4405115229660481 1104 32-33 0.3696903465401391 1104 34-35 0.4239778034056698 1104 36-37 0.43048437877653356 1104 38-39 0.2721841569766852 1104 40-41 0.2837358766485494 1104 42-43 0.3262236783694874 1104 44-45 0.20412909657201084 1104 46-47 0.2607901577052516 1104 48-49 0.4047467375184439 1104 50-51 0.35070192566099223 1104 52-53 0.24539295125642724 1104 54-55 0.35118773777102774 1104 56-57 0.3642882241149863 1104 58-59 0.3474746001151061 1104 60-61 0.4221028382933625 1104 62-63 0.28554857822633295 1104 64-65 0.2859567574991928 1104 66-67 0.31095801098518905 1104 68-69 0.4097478372469112 1104 70-71 0.32160374326443275 1104 72-73 0.2767092887497 1104 74-75 0.3255753977700664 1104 76-77 0.3677325615711524 1104 78-79 0.3449317021470577 1104 80-81 0.4032752286524044 1104 82-83 0.4620968285725553 1104 84-85 0.4679578526347967 1104 86-87 0.44548910212271053 1104 88-89 0.46549719483894947 1104 90-91 0.49694865189225723 1104 92-93 0.3482302117438998 1104 94-95 0.37712265953765467 1104 96-97 0.32520235279237397 1104 98-99 0.2563265604793301 1104 100-101 0.24554272395103283 1104 102-103 0.23696012721219617 1104 104-105 0.14155684472191155 1104 106-107 0.16863003883217687 1104 108-109 0.3200692047410243 1104 110-111 0.25418539204205715 1104 112-113 0.23484226480156778 1104 114-115 0.3413146172538255 1104 116-117 0.17958907374859834 1104 118-119 0.30095954414963444 1104 120-121 0.22092167689430298 1104 122-123 0.2765719274569669 1104 124-125 0.5207560823388384 1105 1 0.050486273651710434 1105 2 0.19295676109775073 1105 3 0.09088253373173316 1105 4 0.17897219856966728 1105 5 0.1381572208343087 1105 6 0.23855772793613284 1105 7 0.2195618467162177 1105 8 0.17755687377558615 1105 9 0.1350384263675366 1105 10-11 0.2960617758067414 1105 12-13 0.37695631907575233 1105 14-15 0.4337407600549241 1105 16-17 0.5415324265014902 1105 18-19 0.5072837525600278 1105 20-21 0.4138400097602073 1105 22-23 0.4274349787545191 1105 24-25 0.5055985203576014 1105 26-27 0.6529431510099002 1105 28-29 0.46726794475763 1105 30-31 0.5016203553423182 1105 32-33 0.560422731919779 1105 34-35 0.5755660815936139 1105 36-37 0.5538231810735041 1105 38-39 0.4810267875481884 1105 40-41 0.5323788900475037 1105 42-43 0.6063120932815309 1105 44-45 0.546308478579121 1105 46-47 0.5883184140173512 1105 48-49 0.5098989570061789 1105 50-51 0.49274572294474694 1105 52-53 0.5107368123447529 1105 54-55 0.5622327655715935 1105 56-57 0.5950511445415714 1105 58-59 0.3921594510858455 1105 60-61 0.4390019359143622 1105 62-63 0.38406512184131714 1105 64-65 0.3580969452669507 1105 66-67 0.40542638214551374 1105 68-69 0.4039085792029766 1105 70-71 0.25081712399545353 1105 72-73 0.4265509166778685 1105 74-75 0.4245966811535311 1105 76-77 0.45026264087112366 1105 78-79 0.32635773441394633 1105 80-81 0.3666343116895092 1105 82-83 0.2666850611858109 1105 84-85 0.2720379273772977 1105 86-87 0.23532822344255422 1105 88-89 0.346179220178481 1105 90-91 0.31501332238719826 1105 92-93 0.3647271126645073 1105 94-95 0.33913842838278896 1105 96-97 0.2847491124314203 1105 98-99 0.37316299289205546 1105 100-101 0.42068286614408734 1105 102-103 0.37892189004147525 1105 104-105 0.3878766879449529 1105 106-107 0.2918127477921608 1105 108-109 0.28220004969590207 1105 110-111 0.2993077601033107 1105 112-113 0.3405664467357603 1105 114-115 0.47613482507444616 1105 116-117 0.37103698187000944 1105 118-119 0.2604755408682564 1105 120-121 0.26484770916118805 1105 122-123 0.2634646446204023 1105 124-125 0.4043753968953965 1106 1 -0.07752655023014299 1106 2 0.0753103463537883 1106 3 0.16474730484646471 1106 4 -0.47226420233431554 1106 5 0.1107825252263126 1106 6 -0.04462669420082932 1106 7 0.07094847273889116 1106 8 0.17647858152422202 1106 9 0.1991867419191209 1106 10-11 0.260397763263164 1106 12-13 0.25260846252103164 1106 14-15 0.2609428938647653 1106 16-17 0.31611262831996356 1106 18-19 0.18885577302218337 1106 20-21 0.2821284945449847 1106 22-23 0.32594039211448234 1106 24-25 0.3647096957049172 1106 26-27 0.3717028959337796 1106 28-29 0.2616200496611967 1106 30-31 0.26997541219135 1106 32-33 0.4345800697379687 1106 34-35 0.43469127211235303 1106 36-37 0.3928536815903101 1106 38-39 0.39464186962453596 1106 40-41 0.30884588431880644 1106 42-43 0.288434866183195 1106 44-45 0.2347818760318674 1106 46-47 0.243067148272182 1106 48-49 0.1807960155720778 1106 50-51 0.1554016417268258 1106 52-53 0.15821035870869338 1106 54-55 0.3077606119653069 1106 56-57 0.18390995920844944 1106 58-59 0.15437783373506164 1106 60-61 0.2361918940440333 1106 62-63 0.19894529212565004 1106 64-65 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-1.8725657277927112 2216 110-111 -2.084048615953943 2216 112-113 -2.4168058694646284 2216 114-115 -2.4444206401174853 2216 116-117 -2.414226518455301 2216 118-119 -2.4745277122221765 2216 120-121 -2.2561835205069976 2216 122-123 -2.172754451020456 2216 124-125 -2.260552221842403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21627.0 3 4869.0 4 3360.0 5 2510.0 6 2877.0 7 3166.0 8 3495.0 9 3976.0 10 4671.0 11 5252.0 12 5234.0 13 5123.0 14 5129.0 15 5137.0 16 5044.0 17 4960.0 18 4718.0 19 4782.0 20 4689.0 21 4792.0 22 5699.0 23 7268.0 24 10294.0 25 14934.0 26 17955.0 27 16330.0 28 18694.0 29 23201.0 30 27019.0 31 33901.0 32 44940.0 33 61076.0 34 101957.0 35 147299.0 36 304046.0 37 432912.0 38 69451.0 39 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 65.51628846108447 11.465246330079669 7.624310696545393 15.394154512290466 2 12.551568443371435 29.648440154258115 41.94519981861775 15.854791583752698 3 35.23517548293078 20.01925968756045 20.08623181925012 24.65933301025866 4 14.708882086411492 15.362636372145587 44.87608205035759 25.052399491085325 5 16.968454562660014 33.489478730417986 28.52802055379044 21.014046153131563 6 32.356963954077614 37.83598297702425 14.541902682872553 15.265150386025574 7 42.785313993051034 24.37096018345724 16.402798998416657 16.440926825075078 8 40.150445367368285 25.656751358909464 15.732084959830631 18.460718313891622 9 50.75309128948556 11.661875286390979 11.784369170362165 25.800664253761298 10-11 37.829674576661276 24.984696929888717 20.041787953173646 17.143840540276365 12-13 29.23125555884455 27.37343165194822 24.12696244976676 19.268350339440467 14-15 25.33335736533472 27.809265861640835 22.331321346448334 24.526055426576114 16-17 24.051781568517892 32.2905524425641 21.421594555642518 22.236071433275495 18-19 24.385953480053324 26.04721179102419 24.519920333915415 25.046914395007075 20-21 25.02542423992441 27.12650283774032 22.19316044619108 25.654912476144194 22-23 25.569471778920377 24.338813785033288 22.44008926039898 27.651625175647354 24-25 22.831937975253048 23.725824442070515 31.154485792581184 22.287751790095257 26-27 19.986974066676975 23.445848096404383 29.770011514675492 26.79716632224315 28-29 22.741995531099622 24.41783317260729 23.27585788536293 29.564313410930154 30-31 23.168154917675377 27.53134963026535 22.18290063021613 27.11759482184315 32-33 29.064082949225696 24.676278542912026 20.64909280350165 25.610545704360632 34-35 28.258375441328067 19.886538913471625 25.683369600858498 26.171716044341814 36-37 25.190205203251807 25.15883650913921 27.65200645259237 21.998951835016616 38-39 23.553833878328287 28.383434279292985 24.432226636561964 23.63050520581676 40-41 21.87285315083801 27.94607496182031 25.98762860298048 24.193443284361198 42-43 22.53603240438094 22.666879719602 24.158643097743465 30.638444778273595 44-45 23.562325955739986 23.908456098732707 23.492586936342693 29.036631009184617 46-47 24.810557350638224 22.64265130282332 23.990881241936858 28.555910104601594 48-49 19.57198543383424 22.963582505904593 25.62233062811564 31.84210143214553 50-51 23.0961362420846 24.75146800340099 24.80883287273907 27.343562881775334 52-53 24.5834549375711 22.551422128343365 27.61509191200683 25.250031022078705 54-55 21.623567351879107 24.370321125307708 27.67755200790838 26.32855951490481 56-57 21.615538966479043 25.900900198299432 25.72897830213956 26.754582533081965 58-59 23.9254402535561 23.05973361912929 29.13635226107627 23.87847386623834 60-61 25.48189973102626 27.473137287524608 26.748294651027372 20.296668330421763 62-63 26.50165924794162 25.124972184113787 25.14330813901496 23.23006042892963 64-65 29.820435994881088 20.551281322487302 26.96430626401219 22.66397641861942 66-67 29.577950538657856 24.219898434718953 23.655850991372393 22.5463000352508 68-69 24.038787176272354 27.59239494590919 24.97418428761633 23.394633590202126 70-71 24.952634919842513 27.163271114772048 23.263751300481296 24.62034266490415 72-73 23.590589510841458 27.2966091237683 23.618700078612537 25.494101286777703 74-75 21.717823863760447 27.54879799334512 25.883934455097595 24.849443687796835 76-77 22.761165576209315 27.351796932300136 23.72396385754315 26.1630736339474 78-79 23.190763819227854 28.69550870328686 21.081406529354226 27.032320948131062 80-81 22.29862475442043 23.2305099597506 24.91140418133443 29.559461104494538 82-83 24.491419353429883 19.91269442111528 27.391865413257843 28.204020812196994 84-85 25.739160641333918 20.863876015431508 30.22345733454256 23.17350600869201 86-87 27.49669060568762 23.027006529359586 25.097184753111733 24.379118111841063 88-89 23.55218646278309 25.388227234876346 26.79272336976456 24.266862932576004 90-91 20.89074012517682 27.126842999349403 26.40446108354168 25.5779557919321 92-93 23.998190424851497 22.682261830270864 27.033111411936613 26.286436332941026 94-95 22.76455936671803 22.168855547717143 28.269758428650917 26.796826656913915 96-97 22.32265417668444 21.366285921871057 28.261080560939966 28.04997934050453 98-99 21.292873062804812 23.45143970301251 29.40082565849905 25.85486157568363 100-101 20.493237481889206 27.132879494769536 26.62952076588724 25.74436225745402 102-103 21.687483232479803 25.907560444527324 26.107384225255647 26.297572097737227 104-105 22.00367622209428 22.973961768815336 25.849359468732874 29.17300254035751 106-107 21.225069003155273 24.620443651380427 24.07250851209336 30.081978833370943 108-109 23.024482832485628 24.902999679034135 25.76028355913016 26.312233929350075 110-111 24.16848697523294 25.91452741415896 24.086893708999728 25.830091901608366 112-113 21.552269763682254 26.49643987862797 25.658315696214995 26.292974661474776 114-115 22.06537797334106 23.38102788117104 27.287976024009268 27.26561812147863 116-117 24.018298520437483 23.310613848949167 25.74784006610649 26.92324756450686 118-119 21.968566184696297 23.998974614778103 26.833144918220675 27.199314282304925 120-121 20.340215612233507 24.275405772074397 28.16723362336282 27.217144992329274 122-123 21.426838263840363 25.584041851075394 28.90100717668852 24.08811270839573 124-125 22.07002378882363 27.166169415620562 25.016105930109646 25.74770086544616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 3.0 2 3.5 3 6.5 4 11.5 5 18.5 6 23.5 7 27.5 8 33.5 9 37.0 10 48.5 11 64.0 12 86.5 13 114.5 14 148.0 15 200.5 16 260.0 17 316.0 18 400.5 19 503.0 20 618.0 21 769.5 22 960.0 23 1211.0 24 1483.5 25 1767.0 26 2251.5 27 2789.5 28 3217.5 29 3720.5 30 4553.5 31 5447.5 32 6275.0 33 7253.5 34 8616.0 35 9835.5 36 10997.0 37 12586.5 38 13783.0 39 14579.5 40 15332.5 41 16634.5 42 18370.0 43 20586.0 44 23313.5 45 28873.0 46 47688.0 47 83009.5 48 114303.0 49 136196.0 50 136260.0 51 109169.0 52 82012.5 53 64703.5 54 55439.0 55 50812.0 56 46730.5 57 41723.0 58 37245.5 59 32188.0 60 28619.0 61 25011.0 62 19659.5 63 15194.5 64 12207.5 65 10783.0 66 9439.0 67 8107.0 68 7201.5 69 6599.0 70 5911.5 71 4423.5 72 3088.5 73 2337.0 74 1780.5 75 1410.0 76 1106.5 77 891.0 78 710.0 79 529.0 80 505.0 81 500.0 82 409.5 83 209.5 84 59.5 85 50.5 86 41.0 87 25.0 88 18.0 89 17.5 90 17.5 91 13.0 92 8.5 93 5.5 94 4.0 95 3.5 96 4.0 97 3.0 98 2.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15937376301627498 2 0.01823197027980875 3 0.009012000518536645 4 0.0163602470951896 5 0.004298031016532861 6 0.0 7 3.466154045591017E-4 8 2.772923236472814E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.70523132765485E-4 28-29 0.007486892738476598 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.4661540455910174E-5 52-53 0.0 54-55 0.0 56-57 3.8127694501501194E-4 58-59 0.0 60-61 6.932308091182035E-5 62-63 0.0 64-65 0.0018717231846191494 66-67 3.4661540455910174E-5 68-69 0.00592712341796064 70-71 0.018474601063000123 72-73 2.0796924273546103E-4 74-75 0.008353431249874352 76-77 4.852615663827425E-4 78-79 0.0013864616182364068 80-81 0.0010398462136773053 82-83 1.0398462136773052E-4 84-85 0.019202493412574236 86-87 0.0025302924532814427 88-89 0.01802400103707329 90-91 1.0398462136773052E-4 92-93 0.013414016156437239 94-95 0.013414016156437239 96-97 0.005892461877504729 98-99 0.0024956309128255326 100-101 7.625538900300239E-4 102-103 0.0 104-105 5.199231068386527E-4 106-107 0.001005184673221395 108-109 0.0 110-111 0.0 112-113 9.70523132765485E-4 114-115 0.005407200311121988 116-117 0.0 118-119 0.07705260443348833 120-121 0.0016637539418836883 122-123 0.0050605849065628854 124-125 0.2502563220916714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1442521.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.204613488659064 #Duplication Level Percentage of deduplicated Percentage of total 1 81.39556936309316 36.79455252747966 2 10.221807194598014 9.241456867747953 3 3.01740198477087 4.092014713844398 4 1.3721306277903913 2.4810653874086297 5 0.7832116811388783 1.770239066284294 6 0.5565657604150638 1.5095604048350748 7 0.38452803025981513 1.216770868841524 8 0.2929021188017166 1.0592421656352717 9 0.20863847498020915 0.8488279458309258 >10 1.4877467373529696 13.021134296438936 >50 0.1510516328720736 4.774129988087824 >100 0.11191218189615151 10.258171473921486 >500 0.009798051573842798 2.9869916304908326 >1k 0.005664498566127869 4.987731373256059 >5k 4.592836675238812E-4 1.5594835476814815 >10k+ 6.123782233651749E-4 3.3986277422157576 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 13844 0.9597087321432409 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 13082 0.9068845444884338 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11603 0.8043557078198516 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 10580 0.7334381960470593 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9062 0.628205759222916 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 7384 0.5118816294528814 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6088 0.42203891659116227 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 4614 0.3198566953271391 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 4075 0.28249155471566795 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3777 0.26183327660394545 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3320 0.23015262862724353 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3267 0.22647850533891709 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2743 0.1901532109411232 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2674 0.1853699183582076 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2480 0.17192124066131445 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2460 0.17053477904307807 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2330 0.16152277852454142 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2321 0.16089887079633503 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2280 0.1580566244789504 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2223 0.15410520886697665 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1897 0.1315058844897232 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1744 0.12089945311021467 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1687 0.11694803749824094 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1666 0.11549225279909271 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1628 0.11285797572444353 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1604 0.11119422178255983 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1568 0.1086985908697343 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1543 0.1069655138469388 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1476 0.10232086742584684 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1465 0.1015583135358168 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1460 0.10121169813125772 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.932308091182035E-5 0.0 0.0 0.0 0.0 3 6.932308091182035E-5 0.0 0.0 0.0 0.0 4 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 5 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 6 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 7 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 8 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 9 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 10-11 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 12-13 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 14-15 6.932308091182035E-5 0.0 0.0 6.932308091182035E-5 0.0 16-17 6.932308091182035E-5 0.0 0.0 1.386461618236407E-4 0.0 18-19 6.932308091182035E-5 0.0 0.0 1.386461618236407E-4 0.0 20-21 6.932308091182035E-5 0.0 0.0 5.545846472945628E-4 0.0 22-23 6.932308091182035E-5 0.0 0.0 0.0017677385632514188 0.0 24-25 6.932308091182035E-5 0.0 0.0 0.002807584776928724 0.0 26-27 6.932308091182035E-5 0.0 0.0 0.004471338718812412 0.0 28-29 6.932308091182035E-5 0.0 0.0 0.010953046784067614 0.0 30-31 6.932308091182035E-5 0.0 0.0 0.03563206358867566 0.0 32-33 6.932308091182035E-5 0.0 0.0 0.08876820510758596 0.0 34-35 6.932308091182035E-5 0.0 0.0 0.15358528576013797 0.0 36-37 6.932308091182035E-5 0.0 0.0 0.23975387533353065 0.0 38-39 6.932308091182035E-5 0.0 0.0 0.38207416044549786 0.0 40-41 6.932308091182035E-5 0.0 0.0 0.5803728333937599 0.0 42-43 6.932308091182035E-5 0.0 0.0 0.9683047941763065 0.0 44-45 6.932308091182035E-5 0.0 0.0 1.4981411015853494 0.0 46-47 6.932308091182035E-5 0.0 0.0 2.036677455648826 0.0 48-49 6.932308091182035E-5 0.0 0.0 2.2934848088866646 0.0 50-51 6.932308091182035E-5 0.0 0.0 2.604225519073899 0.0 52-53 6.932308091182035E-5 0.0 0.0 2.8602009953407954 0.0 54-55 6.932308091182035E-5 0.0 0.0 3.121583671918814 0.0 56-57 6.932308091182035E-5 0.0 0.0 3.503415201581121 0.0 58-59 6.932308091182035E-5 0.0 0.0 3.930861318483405 0.0 60-61 6.932308091182035E-5 0.0 0.0 4.506520182375161 0.0 62-63 6.932308091182035E-5 0.0 0.0 5.164118927904688 0.0 64-65 6.932308091182035E-5 0.0 0.0 5.755652777325253 0.0 66-67 6.932308091182035E-5 0.0 0.0 6.279561961316334 0.0 68-69 6.932308091182035E-5 0.0 0.0 6.741600295593617 0.0 70-71 6.932308091182035E-5 0.0 0.0 7.279512741928887 0.0 72-73 6.932308091182035E-5 0.0 0.0 7.783006278591438 0.0 74-75 6.932308091182035E-5 0.0 0.0 8.295858431177084 0.0 76-77 6.932308091182035E-5 0.0 0.0 8.860356279042039 0.0 78-79 6.932308091182035E-5 0.0 0.0 9.568248919773092 0.0 80-81 6.932308091182035E-5 0.0 0.0 11.324722482376341 0.0 82-83 6.932308091182035E-5 0.0 0.0 12.57063155406403 0.0 84-85 6.932308091182035E-5 0.0 0.0 13.188265543447894 0.0 86-87 6.932308091182035E-5 0.0 0.0 13.703717311567734 0.0 88-89 6.932308091182035E-5 0.0 0.0 14.287694945168909 0.0 90-91 6.932308091182035E-5 0.0 0.0 15.013992863882052 0.0 92-93 6.932308091182035E-5 0.0 0.0 15.911518792447389 0.0 94-95 6.932308091182035E-5 0.0 0.0 16.95476183708937 0.0 96-97 6.932308091182035E-5 0.0 0.0 18.07432959381527 0.0 98-99 6.932308091182035E-5 0.0 0.0 19.185821211614943 0.0 100-101 6.932308091182035E-5 0.0 0.0 20.293326752262185 0.0 102-103 6.932308091182035E-5 0.0 0.0 21.3373323507942 0.0 104-105 6.932308091182035E-5 0.0 0.0 22.398530073392344 0.0 106-107 6.932308091182035E-5 0.0 0.0 23.4140438856696 0.0 108-109 6.932308091182035E-5 0.0 0.0 24.29978489047993 0.0 110-111 6.932308091182035E-5 0.0 0.0 25.287118870366534 0.0 112-113 6.932308091182035E-5 0.0 0.0 28.02142915077146 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTA 1980 0.0 89.54967 2 GAGTAGT 2605 0.0 83.95262 1 GAGTCAG 6385 0.0 78.86272 1 GAGTTAG 1455 0.0 74.94869 1 GTGGGGT 7140 0.0 72.81498 7 GAGTACG 615 0.0 72.67116 1 AGTCGTA 475 0.0 72.651955 2 GAGTCGT 870 0.0 70.5495 1 TGGGGTA 7725 0.0 70.07295 8 AGTACGG 405 0.0 69.04874 2 GAGTAAG 1360 0.0 67.915474 1 TCGTAGG 1150 0.0 65.70808 2 GGGGTAA 9510 0.0 64.9894 9 AGTGGGG 6870 0.0 64.76679 6 AGTCAGT 5695 0.0 63.208366 2 GAGTCCG 705 0.0 62.548737 1 GAGTTCG 565 0.0 62.22709 1 GATTGTG 8510 0.0 62.211113 9 GAGTAGG 1420 0.0 61.268948 1 AGTTAGT 1050 0.0 61.19943 2 >>END_MODULE