FastQCFastQC Report
Thu 26 May 2016
SRR935945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681639
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCT10960.16078892199536704No Hit
GTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCG8980.13174128827722592No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8430.12367250113329784No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT8170.11985816539253182No Hit
GGTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC8080.11853781840534358No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT7590.11134926258620766No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG602.1956146E-549.5711982
TTAAGGT751.4724119E-647.5883524
TAAGGTA1004.150934E-947.588355
GTCTAAG1056.350092E-945.355511
GTTAGAG1404.0017767E-1142.48963
AGTTAGA1404.0017767E-1142.48962
AGTATCT853.4846562E-641.9897232
TAAGGGT853.4846562E-641.9897234
GGAAACA3150.041.5758821
GTCTTAG1104.678368E-737.882161
TGTTAGG801.19868404E-437.1784022
AGTAAGG3950.036.143052
AGCGTAA609.3079325E-734.6998410-11
CACTAGG350.002788501334.00165666-67
AAGGTAG1408.074858E-833.991686
GTGTTAG902.3851158E-433.0717281
CTTAAGG1102.0419746E-532.44663
AGTTAGG2750.032.44662
TGAAAGT651.7397124E-632.0306216-17
GAAAGTG651.7397124E-632.0306218-19