FastQCFastQC Report
Thu 26 May 2016
SRR935945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681639
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT77051.1303637262539261No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT45460.6669219337508564RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT24290.35634698132002424RNA PCR Primer, Index 39 (96% over 25bp)
TCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCT12130.17795343282881407No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT10160.14905250433147166RNA PCR Primer, Index 39 (96% over 28bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA9200.13496880313479717No Hit
GTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCG8870.1301275308484403No Hit
GGTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC8320.12205874370451221No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC7730.11340313567738936No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7490.10988221037822074No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT7390.1084151581702338No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7200.10562775897505865No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG7150.10489423287106518Illumina PCR Primer Index 11 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG7120.1044541172086691RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT453.152786E-452.9472854
ATACGAG453.152786E-452.9472853
GTGATAG803.7556674E-852.215861
TAGGGAG1750.047.6175845
CTATACA653.4580622E-545.904051
TCCTAAC653.4958117E-545.8197632
CCTAACG705.4142598E-542.5469253
GCGTCAG853.4091372E-642.1237141
TGTATGG853.4524965E-642.046372
ATAAGGG1850.041.8569763
CTTTACA1202.0207153E-839.7835121
GTCATGT905.049329E-639.783511
TATAAGG1800.039.7104642
TATACGG650.00192787336.655812
GTCCTAA2300.036.3240741
TATTAGG851.698698E-435.0386432
ACGCGTA851.710467E-434.997527
GTCTGAG1554.96766E-934.650151
GTGTAGG2250.034.4790421
GTATTGG2800.034.100151
Produced by FastQC (version 0.11.5)