##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935945_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 681639 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33847535132233 33.0 31.0 34.0 31.0 34.0 2 32.436886680486296 34.0 31.0 34.0 31.0 34.0 3 32.42882522860341 34.0 31.0 34.0 30.0 34.0 4 35.9427468205311 37.0 35.0 37.0 35.0 37.0 5 35.93180701221615 37.0 35.0 37.0 35.0 37.0 6 36.00189103029609 37.0 35.0 37.0 35.0 37.0 7 35.98952524723497 37.0 35.0 37.0 35.0 37.0 8 35.99683263428295 37.0 35.0 37.0 35.0 37.0 9 37.66062241156976 39.0 37.0 39.0 35.0 39.0 10-11 37.61134339437737 39.0 37.0 39.0 35.0 39.0 12-13 37.594233164475625 39.0 37.0 39.0 35.0 39.0 14-15 38.734123194242116 40.0 38.0 41.0 34.0 41.0 16-17 38.65751152736273 40.0 38.0 41.0 34.5 41.0 18-19 38.70754460939001 40.0 38.0 41.0 34.0 41.0 20-21 38.74638408307037 40.0 38.0 41.0 34.5 41.0 22-23 38.48394091300527 40.0 38.0 41.0 34.0 41.0 24-25 38.1197789445733 40.0 38.0 41.0 33.0 41.0 26-27 37.90947627703227 40.0 37.0 41.0 33.0 41.0 28-29 37.80440159673962 40.0 37.0 41.0 33.0 41.0 30-31 37.64366402157154 40.0 37.0 41.0 33.0 41.0 32-33 37.35232432416572 40.0 35.5 41.0 32.0 41.0 34-35 37.20154436585935 40.0 35.0 41.0 32.0 41.0 36-37 37.16774054888292 40.0 35.0 41.0 32.0 41.0 38-39 36.856591245512654 39.5 35.0 41.0 31.5 41.0 40-41 36.59158660229241 39.0 35.0 41.0 31.0 41.0 42-43 36.33517961853708 39.0 35.0 41.0 31.0 41.0 44-45 35.98361376036289 38.5 35.0 41.0 30.0 41.0 46-47 35.56259545008429 38.0 35.0 41.0 29.5 41.0 48-49 35.059100198198756 37.0 35.0 40.0 28.0 41.0 50-51 34.52866253251355 36.5 34.0 40.0 27.0 41.0 52-53 34.137266206892505 36.0 34.0 39.0 26.5 41.0 54-55 33.725315746311466 35.0 34.0 39.0 26.0 41.0 56-57 33.34257796282196 35.0 34.0 38.5 26.0 40.5 58-59 33.02508072454775 35.0 33.0 37.0 26.0 39.5 60-61 32.61596240825422 35.0 33.0 36.5 25.0 39.0 62-63 32.25233004566934 35.0 33.0 36.0 24.5 39.0 64-65 31.9332131817575 35.0 33.0 35.0 23.5 37.0 66-67 31.545656865290866 35.0 33.0 35.0 22.5 37.0 68-69 31.13234644731302 35.0 33.0 35.0 20.0 36.0 70-71 30.726498923917205 35.0 32.5 35.0 16.5 36.0 72-73 30.488653084697326 35.0 32.0 35.0 13.0 35.0 74-75 30.38988159421629 35.0 32.0 35.0 11.5 35.0 76-77 30.303017432981388 35.0 32.0 35.0 10.5 35.0 78-79 30.44887616465607 35.0 32.0 35.0 12.0 35.0 80-81 30.456872332715704 35.0 33.0 35.0 10.5 35.0 82-83 30.382233851056057 35.0 32.0 35.0 9.0 35.0 84-85 30.336149340046568 35.0 32.0 35.0 7.5 35.0 86-87 30.29154508471493 35.0 32.0 35.0 7.0 35.0 88-89 30.217025434284132 35.0 32.0 35.0 7.0 35.0 90-91 30.123589612683546 35.0 32.0 35.0 5.0 35.0 92-93 30.02212974903138 35.0 32.0 35.0 2.0 35.0 94-95 29.98935433565274 35.0 32.0 35.0 2.0 35.0 96-97 29.951309270743018 35.0 32.0 35.0 2.0 35.0 98-99 29.865931966920908 35.0 32.0 35.0 2.0 35.0 100-101 29.76059835191355 35.0 31.5 35.0 2.0 35.0 102-103 28.96301781441496 33.5 30.0 35.0 2.0 35.0 104-105 29.430288613180878 34.5 31.0 35.0 2.0 35.0 106-107 29.34245252985818 35.0 31.0 35.0 2.0 35.0 108-109 29.05943615315438 35.0 31.0 35.0 2.0 35.0 110-111 28.664594455422886 35.0 30.5 35.0 2.0 35.0 112-113 28.139378762072006 35.0 29.5 35.0 2.0 35.0 114-115 27.462832232310653 34.0 28.0 35.0 2.0 35.0 116-117 26.781401152222806 34.0 26.0 35.0 2.0 35.0 118-119 26.172666910197332 34.0 22.0 35.0 2.0 35.0 120-121 25.48684494285098 34.0 8.5 35.0 2.0 35.0 122-123 24.71931110162417 34.0 2.0 35.0 2.0 35.0 124-125 22.30579162870669 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029827489059741197 1101 2 0.06794164273492243 1101 3 -0.02317871439770869 1101 4 0.09693722520058401 1101 5 0.14564194654282403 1101 6 -0.008261996328684518 1101 7 0.1410437654145511 1101 8 0.05461333579751937 1101 9 0.21279967388130672 1101 10-11 0.1477313549953294 1101 12-13 0.07348461114305138 1101 14-15 0.054223167203453215 1101 16-17 0.1663939838820312 1101 18-19 0.2035629687731202 1101 20-21 0.12350094725930916 1101 22-23 0.03016724804147941 1101 24-25 0.14494310539807742 1101 26-27 0.34023121738351847 1101 28-29 0.2535742706736883 1101 30-31 0.29139495746390054 1101 32-33 0.3356606137013287 1101 34-35 0.44160476656399794 1101 36-37 0.44079734551443295 1101 38-39 0.42779486239851394 1101 40-41 0.3038700904021141 1101 42-43 0.44391242738301884 1101 44-45 0.5995442908396313 1101 46-47 0.5459955446262157 1101 48-49 0.4077982767796442 1101 50-51 0.4362108852649058 1101 52-53 0.6818794286944367 1101 54-55 0.6142087463008252 1101 56-57 0.44313929277589637 1101 58-59 0.5214848717628584 1101 60-61 0.5275387130164901 1101 62-63 0.5038898497015154 1101 64-65 0.39642593447854324 1101 66-67 0.2613141223272599 1101 68-69 0.30128045502377176 1101 70-71 0.4850637909502957 1101 72-73 0.280205800570716 1101 74-75 0.5017614293856774 1101 76-77 0.5201262557850335 1101 78-79 0.4024964997656504 1101 80-81 0.3269019519032348 1101 82-83 0.5165265556028835 1101 84-85 0.5710297258190131 1101 86-87 0.3570060516976774 1101 88-89 0.2209231913483123 1101 90-91 0.14080671845946924 1101 92-93 0.1095140590644732 1101 94-95 0.13857514467677845 1101 96-97 0.1454741710968257 1101 98-99 0.35674998600029895 1101 100-101 0.2643279075502001 1101 102-103 0.1621884394398947 1101 104-105 0.2310734180652254 1101 106-107 0.011321467498380144 1101 108-109 0.03681461194437574 1101 110-111 0.17421211498216493 1101 112-113 -0.01099436149202404 1101 114-115 0.05082669991710276 1101 116-117 -0.2779890883186802 1101 118-119 -0.7027546349703258 1101 120-121 -0.33804351542461575 1101 122-123 -0.6031109869064117 1101 124-125 -0.6985358200696581 1102 1 0.17113061695707188 1102 2 0.12365456910002592 1102 3 0.12909035308875616 1102 4 0.17553235524498945 1102 5 0.16556704291719626 1102 6 0.26025164247480603 1102 7 0.22021104204559805 1102 8 0.2579961982383523 1102 9 0.34593293889862764 1102 10-11 0.26057781476207253 1102 12-13 0.31337018182572507 1102 14-15 0.5040163892792862 1102 16-17 0.2575171722878338 1102 18-19 0.3405036314384233 1102 20-21 0.3827968043812575 1102 22-23 0.4359686192414003 1102 24-25 0.47306002337006703 1102 26-27 0.5204384832791931 1102 28-29 0.3897604074033012 1102 30-31 0.6325773124143694 1102 32-33 0.75094815090916 1102 34-35 0.5897181467425909 1102 36-37 0.5569790905005263 1102 38-39 0.5906797499210796 1102 40-41 0.6504262315234755 1102 42-43 0.5498479907461231 1102 44-45 0.6236805812802686 1102 46-47 0.7106578683465159 1102 48-49 0.702709343973325 1102 50-51 0.6829469658777967 1102 52-53 1.0061694301583515 1102 54-55 0.8072856506096144 1102 56-57 0.7357532341217237 1102 58-59 0.6962677244442688 1102 60-61 0.7285335649171358 1102 62-63 0.8231744834789012 1102 64-65 0.6863786012428008 1102 66-67 0.7558445717490123 1102 68-69 0.7110905121164386 1102 70-71 0.7356145887837044 1102 72-73 0.816500510959628 1102 74-75 0.7926528313278034 1102 76-77 0.9026422368517402 1102 78-79 0.9022348250474543 1102 80-81 0.7593746254990954 1102 82-83 0.7551555996667112 1102 84-85 0.7442065422929254 1102 86-87 0.6220685120499923 1102 88-89 0.7248964993071283 1102 90-91 0.7074172684033542 1102 92-93 0.7330342854832494 1102 94-95 0.7869538593598406 1102 96-97 0.90087479570035 1102 98-99 0.6391891115553641 1102 100-101 0.7297478070280725 1102 102-103 0.7825941877441949 1102 104-105 0.822624801464432 1102 106-107 0.8808195888080981 1102 108-109 0.7890653804994905 1102 110-111 0.6423677046957259 1102 112-113 0.6041534938732234 1102 114-115 0.4860997199727315 1102 116-117 0.8487877379146695 1102 118-119 0.8615368304080988 1102 120-121 0.9451678250331028 1102 122-123 0.9582825377117494 1102 124-125 0.6577895105310354 1103 1 0.008784631969113832 1103 2 0.1317282602674652 1103 3 0.19796869893211522 1103 4 0.252473770530834 1103 5 0.2229916286699236 1103 6 0.2762479602768195 1103 7 0.20911133945389793 1103 8 0.20884475604414376 1103 9 0.35569076356935625 1103 10-11 0.29979853420691427 1103 12-13 0.34059838368570894 1103 14-15 0.2985338089082319 1103 16-17 0.4921268829048415 1103 18-19 0.43326315558513784 1103 20-21 0.2887473307467374 1103 22-23 0.47678141723979195 1103 24-25 0.4566742423192238 1103 26-27 0.484685166940622 1103 28-29 0.5605583349867445 1103 30-31 0.5986184774995849 1103 32-33 0.6791753850119733 1103 34-35 0.5953517916465216 1103 36-37 0.7266416737040373 1103 38-39 0.8985209433308938 1103 40-41 0.9340864779474956 1103 42-43 0.769844568190301 1103 44-45 0.6192253577519651 1103 46-47 0.6316475770752135 1103 48-49 0.7219824639280006 1103 50-51 0.5524419618651422 1103 52-53 0.45339479955833895 1103 54-55 0.3284594692377638 1103 56-57 0.45881358912337333 1103 58-59 0.5592439317803368 1103 60-61 0.4561754239550453 1103 62-63 0.595085921896505 1103 64-65 0.6254571075922328 1103 66-67 0.5437859634310236 1103 68-69 0.5293592889658001 1103 70-71 0.5841765252421816 1103 72-73 0.5951832990444146 1103 74-75 0.6371690707650899 1103 76-77 0.8188061418699135 1103 78-79 0.7731684746721541 1103 80-81 0.7184084025844975 1103 82-83 0.9602032794099031 1103 84-85 0.781546036226743 1103 86-87 0.7887977288748047 1103 88-89 0.8606574868915118 1103 90-91 0.8378732944619856 1103 92-93 0.8277423060657938 1103 94-95 0.7144204599357735 1103 96-97 0.8184891562725056 1103 98-99 0.9735952391104803 1103 100-101 0.9116224234657828 1103 102-103 0.9712490052865661 1103 104-105 0.7832400353784195 1103 106-107 1.0010870202389626 1103 108-109 0.8809728062654365 1103 110-111 0.9710257087555867 1103 112-113 0.782505238196503 1103 114-115 0.8547892351499975 1103 116-117 0.448565271786169 1103 118-119 0.3794627615793189 1103 120-121 0.5017532945133496 1103 122-123 0.3723639945403363 1103 124-125 0.6046608224320877 1104 1 0.11537206006530454 1104 2 0.10101349601954013 1104 3 0.20221708759383716 1104 4 0.2460226420164986 1104 5 0.3046743509652998 1104 6 0.23541352961634487 1104 7 0.19951261466353998 1104 8 0.21504985226240336 1104 9 0.39671924787180046 1104 10-11 0.3083923383513465 1104 12-13 0.3549057969968672 1104 14-15 0.35987901647632725 1104 16-17 0.401677671825297 1104 18-19 0.3943657961007716 1104 20-21 0.3217422252878279 1104 22-23 0.37083217150782133 1104 24-25 0.3636705691609947 1104 26-27 0.546953284389275 1104 28-29 0.6590134138787889 1104 30-31 0.5995754622756095 1104 32-33 0.5758903340728949 1104 34-35 0.5687306351792216 1104 36-37 0.4486650294459409 1104 38-39 0.5469933484409637 1104 40-41 0.48130042208777013 1104 42-43 0.1996745402373321 1104 44-45 0.23531332873632493 1104 46-47 0.3682434372641836 1104 48-49 0.23782729031929506 1104 50-51 0.1782638021405205 1104 52-53 0.2470112432943381 1104 54-55 0.34081941564199525 1104 56-57 0.28338274383494877 1104 58-59 0.2680993618170717 1104 60-61 0.21062190904294908 1104 62-63 0.3185028831088772 1104 64-65 0.3712999703454827 1104 66-67 0.4312492896028495 1104 68-69 0.290357394632629 1104 70-71 0.35743466278925595 1104 72-73 0.3695185498125433 1104 74-75 0.3080667998754443 1104 76-77 0.2763008862504428 1104 78-79 0.5262750655654926 1104 80-81 0.4656535339172301 1104 82-83 0.4700260899534854 1104 84-85 0.5530293915066196 1104 86-87 0.5312729523830164 1104 88-89 0.5809788304819676 1104 90-91 0.6031619492544174 1104 92-93 0.5655042207283927 1104 94-95 0.46986320256057823 1104 96-97 0.5588072656355969 1104 98-99 0.49159429194389404 1104 100-101 0.47243975892446244 1104 102-103 0.4693194421382749 1104 104-105 0.4822339596512961 1104 106-107 0.3269111706767376 1104 108-109 0.37888961731725246 1104 110-111 0.4597936991453082 1104 112-113 0.2831312922081004 1104 114-115 0.5672283046165774 1104 116-117 0.5949602633540785 1104 118-119 0.6016385880399184 1104 120-121 0.5816618120821317 1104 122-123 0.6358149151400561 1104 124-125 0.8512677714006003 1105 1 0.16813737341738744 1105 2 0.18717297387341603 1105 3 0.1836048970276778 1105 4 0.2050743198832592 1105 5 0.26955401260087086 1105 6 0.201740257400445 1105 7 0.2880004949478021 1105 8 0.2851788859056228 1105 9 0.2804172103854867 1105 10-11 0.16136698248901382 1105 12-13 0.2729860726694042 1105 14-15 0.36707248106184664 1105 16-17 0.43548244671418956 1105 18-19 0.42288309004413094 1105 20-21 0.5846241197851114 1105 22-23 0.4897422558842308 1105 24-25 0.41354175021602657 1105 26-27 0.5537935703673327 1105 28-29 0.5538848096320592 1105 30-31 0.5245024648734571 1105 32-33 0.6103901945190344 1105 34-35 0.589606818703281 1105 36-37 0.5467933975246027 1105 38-39 0.6341141376717019 1105 40-41 0.628556578559845 1105 42-43 0.6626594898905935 1105 44-45 0.7545266743790222 1105 46-47 0.6692229979915894 1105 48-49 0.5698017626983685 1105 50-51 0.6906636990853627 1105 52-53 0.4804521046636836 1105 54-55 0.5419750289275171 1105 56-57 0.593109110926008 1105 58-59 0.5518450946895186 1105 60-61 0.6132245884770029 1105 62-63 0.6173832317788239 1105 64-65 0.7335860772222702 1105 66-67 0.7672225167321223 1105 68-69 0.551063859196681 1105 70-71 0.5270511043805968 1105 72-73 0.6407650878033948 1105 74-75 0.65618604327884 1105 76-77 0.6118994649480847 1105 78-79 0.607807259566826 1105 80-81 0.7055680840170631 1105 82-83 0.6343410952135891 1105 84-85 0.6106715754171326 1105 86-87 0.6378003156739105 1105 88-89 0.6507761553418732 1105 90-91 0.7025232219855937 1105 92-93 0.7608366809555456 1105 94-95 0.8333398111871553 1105 96-97 0.8617432640897675 1105 98-99 0.9032164445084625 1105 100-101 0.9602856914662787 1105 102-103 0.8328738788774359 1105 104-105 0.9755922695467376 1105 106-107 0.9846617771992676 1105 108-109 1.0745532495821024 1105 110-111 1.0720100290935655 1105 112-113 1.010408703806096 1105 114-115 0.962866709838039 1105 116-117 1.1167521348786487 1105 118-119 1.060522264532608 1105 120-121 0.8928776482244345 1105 122-123 0.7596958005556047 1105 124-125 0.5395876503107928 1106 1 0.11587463160972789 1106 2 0.16433857764740623 1106 3 0.12271688485797227 1106 4 0.18200612437847496 1106 5 0.22011001967354815 1106 6 0.14732372829749352 1106 7 0.1317881647179604 1106 8 0.16905655321632906 1106 9 0.1182455288423725 1106 10-11 0.10502692295622751 1106 12-13 0.24159725434764567 1106 14-15 0.18585374918508535 1106 16-17 0.15552805275532222 1106 18-19 0.2594877577303407 1106 20-21 0.1132817374413051 1106 22-23 0.14792568609413337 1106 24-25 0.09790412087998135 1106 26-27 0.2566291204079718 1106 28-29 0.40377909389756184 1106 30-31 0.24615936291369422 1106 32-33 0.38143035840119666 1106 34-35 0.3971616729734535 1106 36-37 0.33076185182797957 1106 38-39 0.42335167875104673 1106 40-41 0.5439404698907566 1106 42-43 0.5327941117177275 1106 44-45 0.39103154722157285 1106 46-47 0.2781876315491658 1106 48-49 0.38504820839266785 1106 50-51 0.39556143973415203 1106 52-53 0.4794708561755314 1106 54-55 0.5490947736308485 1106 56-57 0.48677750257927244 1106 58-59 0.4015673188797493 1106 60-61 0.5035362904851439 1106 62-63 0.454211071503849 1106 64-65 0.5099798397409003 1106 66-67 0.5175694202074332 1106 68-69 0.705741312343207 1106 70-71 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-1.8003584467328153 2216 44-45 -1.4161112960368882 2216 46-47 -1.5142157413602533 2216 48-49 -1.8669991157503034 2216 50-51 -1.7422973886891029 2216 52-53 -1.7862808563263144 2216 54-55 -1.763332819225237 2216 56-57 -1.60569856904646 2216 58-59 -1.2870445438401958 2216 60-61 -1.2179654239605178 2216 62-63 -1.351041896703098 2216 64-65 -1.3292937951171595 2216 66-67 -1.7255212860197773 2216 68-69 -1.6841993429560276 2216 70-71 -1.7199465841182153 2216 72-73 -1.4202369342712444 2216 74-75 -1.5028518872431427 2216 76-77 -1.6582694584633941 2216 78-79 -1.6519823977365036 2216 80-81 -1.6119895470657823 2216 82-83 -1.65319879871549 2216 84-85 -1.9959645059039026 2216 86-87 -2.12555535043737 2216 88-89 -2.0047378114444747 2216 90-91 -2.0960711036896704 2216 92-93 -2.1474679052818324 2216 94-95 -2.080744994701501 2216 96-97 -2.101299117343128 2216 98-99 -2.5573604334395803 2216 100-101 -2.251133554616686 2216 102-103 -2.3815320340286377 2216 104-105 -2.284562593210321 2216 106-107 -2.2404729625906192 2216 108-109 -2.4484009154223187 2216 110-111 -2.449463269352133 2216 112-113 -2.215285333224859 2216 114-115 -2.4440738560299664 2216 116-117 -2.417440775735482 2216 118-119 -2.2837193205861723 2216 120-121 -2.5946535433524716 2216 122-123 -2.262327506103553 2216 124-125 -2.516949282862967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 32.0 5 129.0 6 550.0 7 2936.0 8 2751.0 9 1909.0 10 2223.0 11 3592.0 12 4644.0 13 5018.0 14 6197.0 15 7784.0 16 4199.0 17 2686.0 18 2998.0 19 4061.0 20 4984.0 21 7658.0 22 7685.0 23 3649.0 24 4610.0 25 5847.0 26 7405.0 27 9235.0 28 11183.0 29 14061.0 30 17639.0 31 23470.0 32 31541.0 33 42675.0 34 62026.0 35 99692.0 36 183570.0 37 89173.0 38 3811.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.27810942275155 19.446580006144536 11.681677664605257 16.593632906498655 2 19.526461414776843 21.161356387738874 35.88406681591587 23.428115381568407 3 28.721948127968027 22.430788144457697 27.09528063975213 21.751983087822147 4 16.27636449092298 21.289142823570884 27.702397038951883 34.73209564655426 5 16.547261958569845 32.49828303758534 28.276609982448832 22.677845021395992 6 32.96710390225797 34.87400074233713 9.826284309252367 22.332611046152536 7 29.171306219274424 37.97978108646952 12.838027166872786 20.010885527383262 8 36.41590343275546 26.83487887283445 12.821889592584931 23.92732810182516 9 34.36423091988575 13.941250427278955 16.694173895566422 35.00034475726888 10-11 30.29184069573484 25.16339293966454 17.21835164948015 27.326414715120467 12-13 33.97546208476921 24.786507227432704 18.87868798586935 22.359342701928732 14-15 35.558778180239095 19.80278417168032 21.75499054484852 22.88344710323206 16-17 31.44831795129093 22.038351678821194 22.197380138166977 24.315950231720894 18-19 30.324482607362548 20.86977124254921 23.529683600850305 25.27606254923794 20-21 36.74466983256533 19.177746578467488 20.22478173930776 23.852801849659425 22-23 33.842840565167194 21.64796908627587 20.212091737708672 24.297098610848263 24-25 27.71870447553617 23.94053157169704 22.835767906472487 25.5049960462943 26-27 25.591552126565524 22.227894824093106 24.43419463968464 27.746358409656725 28-29 26.559293115564103 20.309430651708603 25.43633800296051 27.69493822976678 30-31 26.88351165352921 20.748446024948688 24.89008111331658 27.477961208205514 32-33 24.397078218822575 20.243413302349193 26.211234979219206 29.14827349960903 34-35 25.46890656197782 21.267195685692865 25.988022985773995 27.27587476655532 36-37 25.81425065173794 21.973067855565777 23.889698212690295 28.322983280005985 38-39 26.252019030601243 19.315062665135063 25.72211977307636 28.710798531187333 40-41 25.079697611198892 19.044171474930277 27.669704931789408 28.206425982081424 42-43 24.91326053820277 18.961796493451814 27.605741455521176 28.51920151282424 44-45 24.733913405776374 19.20356669732806 27.851692758190183 28.210827138705387 46-47 23.779449239260078 19.020992050044086 27.747532051423114 29.452026659272722 48-49 22.31056321601317 19.482600027287173 28.02832584403181 30.17851091266785 50-51 23.105192044469288 20.650226879623965 26.6715226094751 29.573058466431647 52-53 22.854986290397118 20.671352431418978 26.833265115405663 29.64039616277824 54-55 21.344876100105775 20.78182146268039 25.528322176401293 32.34498026081254 56-57 21.86413922912275 21.547549362639167 24.870422613729552 31.717888794508532 58-59 21.826289282156683 21.302331586074153 25.628668547427598 31.242710584341566 60-61 22.31393743609154 22.36088310674712 23.151697599462473 32.173481857698874 62-63 20.87615291965395 23.547654990398144 23.173116561699082 32.40307552824883 64-65 20.643258381636027 22.933546936134817 23.349089474047112 33.07410520818204 66-67 21.097531097839177 23.02648469351079 22.591650419063463 33.28433378958657 68-69 20.70575480569627 23.35363733589187 22.6097685138321 33.33083934457976 70-71 19.80278417168032 22.66492967685241 23.861384105076148 33.67090204639113 72-73 19.834252441541636 21.41485449042675 24.403753306368912 34.347139761662696 74-75 19.360101167920263 20.853707020871752 25.072949171042154 34.71324264016584 76-77 18.485664699349655 20.873732283510773 25.708842950594086 34.931760066545486 78-79 18.419867407821442 21.401210904892473 25.938583326364835 34.24033836092125 80-81 17.88079907399665 21.88284414477458 25.656175776327352 34.58018100490142 82-83 17.57843961393054 21.40700576111402 25.979734140798872 35.03482048415657 84-85 17.587975453282457 20.582008951952574 26.799816325063556 35.03019926970141 86-87 17.72976604918439 20.37845545809439 26.83891326640641 35.05286522631481 88-89 17.69624390623189 20.428555290997142 26.713480302623527 35.16172050014744 90-91 17.921216362326685 19.839533829490392 26.67423665605988 35.56501315212304 92-93 17.830552535873096 19.299511911730402 26.983051145841124 35.886884406555374 94-95 17.141037998119238 19.06104257532213 27.617697931016274 36.18022149554236 96-97 17.275713390812438 19.337581916527665 27.54236480013614 35.844339892523756 98-99 17.362782939356464 19.74556913556883 27.92555883686233 34.966089088212385 100-101 17.668956735163334 20.411610837994893 27.16115128389074 34.758281142951034 102-103 17.39681855058176 21.038555599078105 27.076502371489898 34.48812347885024 104-105 16.924941207882764 22.411129644870673 27.11941364857351 33.54451549867305 106-107 16.455276513870622 24.300343950642457 26.492855083743066 32.751524451743855 108-109 15.937174956245167 25.75314792727529 26.095411207398637 32.21426590908091 110-111 15.999383832196967 27.25185492182371 25.58013298955082 31.168628256428498 112-113 15.799418753915194 28.16996973471295 25.133978542894408 30.89663296847745 114-115 15.524381960327508 28.999854760126816 24.69672007288401 30.779043206661672 116-117 15.216493484105472 29.48426685641558 24.830454700503594 30.468784958975352 118-119 15.092522362199409 29.852380875533107 25.16435134583983 29.89074541642765 120-121 15.339825053639213 30.27364251526655 24.743943811776788 29.64258861931745 122-123 15.357107489849586 30.539411477760787 24.51760321879046 29.585877813599172 124-125 14.789043978612733 31.908542799847673 24.308608250108044 28.993804971431548 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 1.0 5 2.0 6 3.5 7 5.0 8 8.0 9 15.5 10 19.5 11 16.0 12 21.0 13 30.0 14 29.5 15 30.5 16 38.5 17 45.0 18 50.5 19 61.0 20 77.0 21 103.0 22 136.5 23 175.0 24 248.5 25 333.0 26 463.5 27 644.5 28 827.5 29 1055.5 30 1317.5 31 1591.5 32 1789.0 33 1955.0 34 2146.0 35 2222.0 36 2210.0 37 2241.5 38 2282.5 39 2318.5 40 2473.5 41 2584.5 42 2767.0 43 3256.0 44 3839.5 45 4962.0 46 6787.5 47 8813.0 48 11540.5 49 17619.5 50 27793.0 51 38680.0 52 50134.5 53 59421.5 54 62085.0 55 60886.5 56 58596.5 57 53126.5 58 44606.5 59 34086.0 60 23213.0 61 15384.5 62 10956.5 63 8605.5 64 7133.5 65 6076.5 66 5176.0 67 4471.5 68 3878.0 69 3538.5 70 3361.0 71 2968.5 72 2397.5 73 1866.0 74 1249.0 75 748.5 76 487.5 77 518.5 78 407.0 79 169.5 80 122.5 81 118.0 82 127.5 83 69.0 84 14.5 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1995191002862219 2 7.335261039934629E-4 3 0.0 4 0.11721747141815535 5 0.02978115982213459 6 0.0011736417663895404 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 7.335261039934627E-5 108-109 0.0 110-111 9.535839351915016E-4 112-113 0.0 114-115 0.0011736417663895404 116-117 0.0053547405591522785 118-119 0.0029341044159738516 120-121 2.2005783119803885E-4 122-123 0.0024206361431784273 124-125 0.030147922874131323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 681639.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.41306132167508 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73887803034296 39.40142069834907 2 9.494771441191997 9.383314469178188 3 3.4187276445070998 5.067893962204055 4 1.858749809832714 3.6738607333966318 5 1.1615593756911606 2.869810232989698 6 0.8277595350064862 2.4541279597726056 7 0.5639767118204724 1.9507471091627173 8 0.44076477481395143 1.7423629477052875 9 0.351551569918188 1.5634115353888713 >10 1.9466222864227394 16.805072736846324 >50 0.119641708686558 4.090013865504298 >100 0.06870511511401414 6.291449278791651 >500 0.006811282963886994 2.232166518553037 >1k 0.0011845709502412162 1.346850703865357 >5k 2.9614273756030406E-4 1.1274972482923267 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 7705 1.1303637262539261 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 4546 0.6669219337508564 RNA PCR Primer, Index 39 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 2429 0.35634698132002424 RNA PCR Primer, Index 39 (96% over 25bp) TCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTTCTTTTCCT 1213 0.17795343282881407 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 1016 0.14905250433147166 RNA PCR Primer, Index 39 (96% over 28bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 920 0.13496880313479717 No Hit GTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCG 887 0.1301275308484403 No Hit GGTATCAACGCAGAGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC 832 0.12205874370451221 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 773 0.11340313567738936 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 749 0.10988221037822074 No Hit GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT 739 0.1084151581702338 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 720 0.10562775897505865 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 715 0.10489423287106518 Illumina PCR Primer Index 11 (96% over 25bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 712 0.1044541172086691 RNA PCR Primer, Index 39 (96% over 30bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.4670522079869257E-4 0.0 0.0 0.0 0.0 2 2.9341044159738515E-4 0.0 0.0 0.0 0.0 3 2.9341044159738515E-4 0.0 0.0 0.0 0.0 4 2.9341044159738515E-4 0.0 0.0 0.0 0.0 5 2.9341044159738515E-4 0.0 0.0 0.0 0.0 6 2.9341044159738515E-4 0.0 0.0 0.0 0.0 7 2.9341044159738515E-4 0.0 0.0 0.0 0.0 8 4.401156623960777E-4 0.0 0.0 0.0 0.0 9 4.401156623960777E-4 0.0 0.0 0.0 0.0 10-11 8.802313247921554E-4 0.0 0.0 0.0 0.0 12-13 0.0029341044159738516 0.0 0.0 3.6676305199673145E-4 0.0 14-15 0.004254451403162085 0.0 0.0 6.601734935941165E-4 0.0 16-17 0.005281387948752932 0.0 0.0 0.001320346987188233 0.0 18-19 0.005501445779950971 0.0 0.0 0.0017604626495843108 0.0 20-21 0.005868208831947703 0.0 0.0 0.003447572688769275 0.0 22-23 0.006161619273545088 0.0 0.0 0.014597169469469911 0.0 24-25 0.006675087546340512 0.0 0.0 0.035282605602085564 0.0 26-27 0.0073352610399346286 0.0 0.0 0.08560249633603711 0.0 28-29 0.008142139754327437 0.0 0.0 0.27727286730952894 0.0 30-31 0.009169076299918286 0.0 0.0 0.7313988782918818 0.0 32-33 0.009535839351915017 0.0 0.0 1.4242142835137073 0.0 34-35 0.01004930762471044 0.0 0.0 2.4044985688905713 0.0 36-37 0.011296302001499326 0.0 0.0 3.829226320677074 0.0 38-39 0.011736417663895405 0.0 0.0 5.827644838396864 0.0 40-41 0.012396591157489522 0.0 0.0 8.0900594009439 0.0 42-43 0.013130117261482985 0.0 0.0 9.957616861711259 0.0 44-45 0.013570232923879062 0.0 0.0 11.723360899244321 0.0 46-47 0.013863643365476448 0.0 1.4670522079869257E-4 13.737916991251968 0.0 48-49 0.013936995975875794 0.0 1.4670522079869257E-4 15.90651356509824 0.0 50-51 0.013936995975875794 0.0 1.4670522079869257E-4 18.050316956629537 0.0 52-53 0.013936995975875794 0.0 1.4670522079869257E-4 19.8274306487745 0.0 54-55 0.013936995975875794 0.0 1.4670522079869257E-4 21.58539930960523 0.0 56-57 0.013936995975875794 0.0 1.4670522079869257E-4 23.516626836199222 0.0 58-59 0.01401034858627514 0.0 1.4670522079869257E-4 25.40303591783921 0.0 60-61 0.014083701196674486 0.0 1.4670522079869257E-4 27.43989120340826 0.0 62-63 0.014230406417473179 0.0 1.4670522079869257E-4 29.418504516320223 0.0 64-65 0.014450464248671217 0.0 1.4670522079869257E-4 31.215643471104208 0.0 66-67 0.014670522079869257 0.0 1.4670522079869257E-4 33.106160298926554 0.0 68-69 0.014670522079869257 0.0 1.4670522079869257E-4 35.17463055957772 0.0 70-71 0.014670522079869257 0.0 1.4670522079869257E-4 37.324962333434556 0.0 72-73 0.014670522079869257 0.0 1.4670522079869257E-4 39.44294560610528 0.0 74-75 0.014670522079869257 0.0 1.4670522079869257E-4 41.347619487734704 0.0 76-77 0.014670522079869257 0.0 1.4670522079869257E-4 43.272612042444756 0.0 78-79 0.014670522079869257 0.0 1.4670522079869257E-4 45.264722235670206 0.0 80-81 0.014670522079869257 0.0 1.4670522079869257E-4 47.2966628963425 0.0 82-83 0.01481722730066795 0.0 1.4670522079869257E-4 49.399242120829356 0.0 84-85 0.01481722730066795 0.0 1.4670522079869257E-4 51.491185216808304 0.0 86-87 0.01481722730066795 0.0 1.4670522079869257E-4 53.31304400129687 0.0 88-89 0.01481722730066795 0.0 1.4670522079869257E-4 55.10966948780806 0.0 90-91 0.01481722730066795 0.0 1.4670522079869257E-4 57.09451777260398 0.0 92-93 0.01481722730066795 0.0 1.4670522079869257E-4 58.902219503285465 0.0 94-95 0.01481722730066795 0.0 1.4670522079869257E-4 60.59417081475679 0.0 96-97 0.01481722730066795 0.0 1.4670522079869257E-4 62.18335511905862 0.0 98-99 0.01481722730066795 0.0 1.4670522079869257E-4 63.70336791175388 0.0 100-101 0.01481722730066795 0.0 1.4670522079869257E-4 65.22792856629388 0.0 102-103 0.01481722730066795 0.0 1.4670522079869257E-4 66.74669436461235 0.0 104-105 0.01481722730066795 0.0 1.4670522079869257E-4 68.21301304649529 0.0 106-107 0.01481722730066795 0.0 1.4670522079869257E-4 69.50753991482296 0.0 108-109 0.01481722730066795 0.0 1.4670522079869257E-4 70.76091596871657 0.0 110-111 0.01481722730066795 0.0 1.4670522079869257E-4 72.04143248845797 0.0 112-113 0.01481722730066795 0.0 1.4670522079869257E-4 73.25835229498313 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGGGT 45 3.152786E-4 52.947285 4 ATACGAG 45 3.152786E-4 52.947285 3 GTGATAG 80 3.7556674E-8 52.21586 1 TAGGGAG 175 0.0 47.617584 5 CTATACA 65 3.4580622E-5 45.90405 1 TCCTAAC 65 3.4958117E-5 45.819763 2 CCTAACG 70 5.4142598E-5 42.546925 3 GCGTCAG 85 3.4091372E-6 42.123714 1 TGTATGG 85 3.4524965E-6 42.04637 2 ATAAGGG 185 0.0 41.856976 3 CTTTACA 120 2.0207153E-8 39.783512 1 GTCATGT 90 5.049329E-6 39.78351 1 TATAAGG 180 0.0 39.710464 2 TATACGG 65 0.001927873 36.65581 2 GTCCTAA 230 0.0 36.324074 1 TATTAGG 85 1.698698E-4 35.038643 2 ACGCGTA 85 1.710467E-4 34.99752 7 GTCTGAG 155 4.96766E-9 34.65015 1 GTGTAGG 225 0.0 34.479042 1 GTATTGG 280 0.0 34.10015 1 >>END_MODULE