##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935943_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607345 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.050246564967193 31.0 28.0 33.0 7.0 34.0 2 27.216534259769983 31.0 27.0 34.0 7.0 34.0 3 27.201804575653046 31.0 27.0 34.0 7.0 34.0 4 29.65753237451531 35.0 31.0 37.0 7.0 37.0 5 30.204868731939836 35.0 31.0 37.0 7.0 37.0 6 30.30272250533058 37.0 31.0 37.0 7.0 37.0 7 30.10796005565206 37.0 31.0 37.0 2.0 37.0 8 29.79983864195803 37.0 31.0 37.0 2.0 37.0 9 30.82624043994764 39.0 31.0 39.0 2.0 39.0 10-11 30.940931431064715 39.0 30.0 39.0 2.0 39.0 12-13 30.84711160872321 38.0 30.0 39.0 2.0 39.0 14-15 32.05663914249726 39.0 30.0 41.0 2.0 41.0 16-17 32.44885361697223 39.5 31.0 41.0 2.0 41.0 18-19 32.71451975401131 40.0 31.0 41.0 2.0 41.0 20-21 32.967204801225 40.0 31.0 41.0 2.0 41.0 22-23 32.90377462562465 39.5 30.5 41.0 2.0 41.0 24-25 32.8005902740617 39.0 30.0 41.0 2.0 41.0 26-27 32.77913706377759 39.0 30.0 41.0 2.0 41.0 28-29 32.576929093019615 39.0 30.0 41.0 2.0 41.0 30-31 32.42840642468448 39.0 30.0 41.0 2.0 41.0 32-33 32.35090516922013 39.0 30.0 41.0 2.0 41.0 34-35 32.04253101614404 38.0 30.0 41.0 2.0 41.0 36-37 31.85760070470655 38.0 30.0 41.0 2.0 41.0 38-39 31.797293959775743 39.0 30.0 41.0 2.0 41.0 40-41 31.472816932715343 38.0 30.0 41.0 2.0 41.0 42-43 30.87731108348632 38.0 30.0 41.0 2.0 41.0 44-45 30.463238357111692 37.5 29.5 41.0 2.0 41.0 46-47 30.190567963842625 37.0 29.0 40.5 2.0 41.0 48-49 29.892716660217832 36.5 28.0 40.0 2.0 41.0 50-51 29.572721435098664 35.5 28.0 40.0 2.0 41.0 52-53 29.21544920926327 35.0 27.0 40.0 2.0 41.0 54-55 28.857519202430247 35.0 26.5 39.0 2.0 41.0 56-57 28.535237797298077 35.0 26.0 39.0 2.0 41.0 58-59 28.12113625698738 35.0 25.0 38.5 2.0 40.5 60-61 27.733161547390694 35.0 23.5 37.0 2.0 40.0 62-63 27.17471453621912 35.0 19.5 37.0 2.0 39.0 64-65 26.62601486799101 35.0 15.5 36.0 2.0 39.0 66-67 26.350205402201382 35.0 15.5 36.0 2.0 37.5 68-69 26.165026467658414 34.5 13.0 35.0 2.0 37.0 70-71 25.96187339979748 34.0 7.5 35.0 2.0 36.5 72-73 25.760014489293567 34.0 4.5 35.0 2.0 36.0 74-75 25.486352073368515 34.0 2.0 35.0 2.0 36.0 76-77 25.258516164618133 34.0 2.0 35.0 2.0 35.0 78-79 25.050468020647244 34.0 2.0 35.0 2.0 35.0 80-81 24.946875334447473 34.0 2.0 35.0 2.0 35.0 82-83 24.811800541702 34.0 2.0 35.0 2.0 35.0 84-85 24.613153150186466 34.0 2.0 35.0 2.0 35.0 86-87 24.52924038231977 34.0 2.0 35.0 2.0 35.0 88-89 24.229784554083757 34.0 2.0 35.0 2.0 35.0 90-91 23.902695337905143 33.0 2.0 35.0 2.0 35.0 92-93 23.702174217290008 33.0 2.0 35.0 2.0 35.0 94-95 23.495947937333803 33.0 2.0 35.0 2.0 35.0 96-97 23.339752529451957 33.0 2.0 35.0 2.0 35.0 98-99 23.096017090780364 33.0 2.0 35.0 2.0 35.0 100-101 22.837727321374178 32.0 2.0 35.0 2.0 35.0 102-103 22.224817854761298 31.0 2.0 34.5 2.0 35.0 104-105 22.65416279050622 32.0 2.0 35.0 2.0 35.0 106-107 22.629172052128524 32.0 2.0 35.0 2.0 35.0 108-109 22.468185298306565 32.0 2.0 35.0 2.0 35.0 110-111 22.337488577332486 32.0 2.0 35.0 2.0 35.0 112-113 22.085502473882222 31.5 2.0 35.0 2.0 35.0 114-115 21.76608929027159 31.0 2.0 35.0 2.0 35.0 116-117 21.47135565452914 31.0 2.0 35.0 2.0 35.0 118-119 21.059597098848265 31.0 2.0 35.0 2.0 35.0 120-121 20.55302505165927 30.0 2.0 35.0 2.0 35.0 122-123 19.8487144868238 29.0 2.0 35.0 2.0 35.0 124-125 17.077637915846843 21.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.877670622804672 1101 2 1.0004340605127702 1101 3 1.078250135731217 1101 4 1.4191708352285133 1101 5 0.9044706151978907 1101 6 0.9946842544971233 1101 7 0.9170968281494929 1101 8 1.08983822818848 1101 9 1.2624080774266098 1101 10-11 1.0732817604308984 1101 12-13 0.9725838519734467 1101 14-15 1.0455804941438487 1101 16-17 1.198202655310567 1101 18-19 1.0943352580705437 1101 20-21 0.8785421678566365 1101 22-23 0.9675570566284222 1101 24-25 1.0279852245651497 1101 26-27 1.0342855943070788 1101 28-29 1.1007146223962891 1101 30-31 0.9120691577825681 1101 32-33 0.8521615940610872 1101 34-35 0.7110086613307018 1101 36-37 0.6483659572760097 1101 38-39 0.9506143678895782 1101 40-41 0.9578601640992517 1101 42-43 0.8878369526596614 1101 44-45 1.1573707088540388 1101 46-47 1.171544839741511 1101 48-49 0.9808743655959162 1101 50-51 1.0442049235854398 1101 52-53 1.1924092104801147 1101 54-55 0.9184013353368918 1101 56-57 0.8996546206965057 1101 58-59 0.9280650558313432 1101 60-61 0.9581792509129627 1101 62-63 0.9308812554870194 1101 64-65 1.0529310663161695 1101 66-67 0.7560934518047411 1101 68-69 0.8739033598905621 1101 70-71 0.7901604349447702 1101 72-73 1.0355983197093117 1101 74-75 1.0109906697568718 1101 76-77 1.212868330393139 1101 78-79 1.139384695690186 1101 80-81 1.212326889035424 1101 82-83 1.2056362689697337 1101 84-85 1.2245291639644016 1101 86-87 1.011190636764077 1101 88-89 1.1461659737159309 1101 90-91 1.168466842791947 1101 92-93 0.6498342945780529 1101 94-95 0.8670454785672739 1101 96-97 0.7871711662333993 1101 98-99 0.7880598780794656 1101 100-101 1.0113204243643956 1101 102-103 1.0330862682802078 1101 104-105 0.8742341356746373 1101 106-107 0.8480637804307278 1101 108-109 0.8355307656844495 1101 110-111 0.8903147016106914 1101 112-113 0.7559669564098463 1101 114-115 0.35494154211884066 1101 116-117 0.4635199795218554 1101 118-119 0.21101017568313551 1101 120-121 0.0996508445305615 1101 122-123 0.230771859283049 1101 124-125 0.08578273838619666 1102 1 -0.34594591857165824 1102 2 -0.20227990211072822 1102 3 -0.13111596537396153 1102 4 -0.6420291440169343 1102 5 -0.5268776064729117 1102 6 -0.5442396831293479 1102 7 -0.7093533197266773 1102 8 -0.4346292497369184 1102 9 -0.3388420789955475 1102 10-11 -0.6692977249173033 1102 12-13 -0.46654274772927096 1102 14-15 -0.4494017881851775 1102 16-17 -0.4215076050674682 1102 18-19 -0.6281489394761479 1102 20-21 -0.6764406028147363 1102 22-23 -0.5260586301487891 1102 24-25 -0.6324100580477321 1102 26-27 -0.5417577033467467 1102 28-29 -0.6740519652199595 1102 30-31 -0.6021309221834912 1102 32-33 -0.6686715485135082 1102 34-35 -0.7450237401843864 1102 36-37 -0.9305043847772865 1102 38-39 -0.9199535437448709 1102 40-41 -0.9314608569025253 1102 42-43 -0.9300487386041993 1102 44-45 -0.6106849865912523 1102 46-47 -0.5511209601174798 1102 48-49 -0.5602791600832617 1102 50-51 -0.6854586695765441 1102 52-53 -0.5189987720860643 1102 54-55 -0.3736540976243852 1102 56-57 -0.4760935350480828 1102 58-59 -0.49560398234786973 1102 60-61 -0.40643698830153596 1102 62-63 -0.37888269202838387 1102 64-65 -0.5123854863530788 1102 66-67 -0.6953836312043009 1102 68-69 -0.6834463451201387 1102 70-71 -0.5801930638755763 1102 72-73 -0.5410973728059254 1102 74-75 -0.4001058419526622 1102 76-77 -0.25327962513108204 1102 78-79 -0.3894542093241711 1102 80-81 -0.4774406600048309 1102 82-83 -0.527862337728493 1102 84-85 -0.5867135452368792 1102 86-87 -0.6729525818393256 1102 88-89 -0.6295132036713795 1102 90-91 -0.5916319244488264 1102 92-93 -0.3501458665138806 1102 94-95 -0.40731822660823624 1102 96-97 -0.44583986114734486 1102 98-99 -0.5332312380574997 1102 100-101 -0.3731881353613957 1102 102-103 -0.5252721689021769 1102 104-105 -0.46048410604872103 1102 106-107 -0.48320020218428184 1102 108-109 -0.1579040818153885 1102 110-111 -0.1177688903914813 1102 112-113 -0.13946750041611367 1102 114-115 -0.316970226626637 1102 116-117 -0.48626927023168776 1102 118-119 -0.47589522935106743 1102 120-121 -0.7330712978273368 1102 122-123 -0.685105066235586 1102 124-125 -0.4390136075921518 1103 1 0.5093055302479534 1103 2 0.5755042285315319 1103 3 0.6343401873997223 1103 4 0.5817905792816092 1103 5 0.5895308162525552 1103 6 0.567851235157665 1103 7 0.7699776356806147 1103 8 0.4019550398246885 1103 9 0.5724812177892673 1103 10-11 0.6358281570924085 1103 12-13 0.5812459423149576 1103 14-15 0.5451753089564733 1103 16-17 0.5560765569481703 1103 18-19 0.5946709406892836 1103 20-21 0.45931302528173035 1103 22-23 0.5590409216810528 1103 24-25 0.714730493214752 1103 26-27 0.660683409264621 1103 28-29 0.630475770164665 1103 30-31 0.8697613175970673 1103 32-33 0.847660933047294 1103 34-35 0.8841070266894349 1103 36-37 0.7305194721835697 1103 38-39 0.7636628097011453 1103 40-41 0.8783277832961502 1103 42-43 0.7797907733785259 1103 44-45 0.8958916941934376 1103 46-47 0.7854728587408495 1103 48-49 0.807946059713629 1103 50-51 0.7463768049164585 1103 52-53 0.9754221911938927 1103 54-55 0.994502951049796 1103 56-57 0.8304485470592731 1103 58-59 1.0054332449493124 1103 60-61 1.039519437889659 1103 62-63 0.7086512286028324 1103 64-65 0.8943446894111347 1103 66-67 0.9116904788693425 1103 68-69 1.1075769107620062 1103 70-71 1.1007829413552734 1103 72-73 0.995555967505787 1103 74-75 0.8518201755196735 1103 76-77 1.0657912830988572 1103 78-79 0.9304252230815919 1103 80-81 0.8630550393502538 1103 82-83 0.8402294719142738 1103 84-85 0.8568583577050326 1103 86-87 0.9629395875710465 1103 88-89 0.9591752279090073 1103 90-91 0.9042594826178068 1103 92-93 1.0977369436610616 1103 94-95 1.203091137318534 1103 96-97 1.0339866176895534 1103 98-99 0.9158617928012944 1103 100-101 0.925762762371658 1103 102-103 0.8481384543578088 1103 104-105 0.8461129527704969 1103 106-107 0.9593388715973958 1103 108-109 0.8937014580779099 1103 110-111 0.9446524012826991 1103 112-113 1.1257760757383615 1103 114-115 0.9251406152207551 1103 116-117 0.9569823017357599 1103 118-119 0.9461565894984716 1103 120-121 0.7847177000864782 1103 122-123 0.7833749522952296 1103 124-125 0.918958088930534 1104 1 0.027277615759143004 1104 2 0.16844637791109562 1104 3 0.06338635217540656 1104 4 0.152906756448008 1104 5 0.15919146609208568 1104 6 0.41000214101182664 1104 7 0.29276447793558447 1104 8 0.09286846994342213 1104 9 0.17721466480821135 1104 10-11 0.26519803447567725 1104 12-13 0.20773667841772436 1104 14-15 0.3372333191437775 1104 16-17 0.2552132308254045 1104 18-19 0.2508924528004144 1104 20-21 0.42982229334158006 1104 22-23 0.3851678453290006 1104 24-25 0.2053056415772545 1104 26-27 0.3735289582509509 1104 28-29 0.36673532856683266 1104 30-31 0.3558796940111506 1104 32-33 0.41632602439585753 1104 34-35 0.3701671265089175 1104 36-37 0.34054221393084205 1104 38-39 0.16491658900767803 1104 40-41 0.3241576073147101 1104 42-43 0.08667670804490868 1104 44-45 -0.29377711436934106 1104 46-47 -0.10975345563917571 1104 48-49 -0.1793225856746794 1104 50-51 -0.08259942794571273 1104 52-53 -0.31663096261052814 1104 54-55 -0.03933632487738947 1104 56-57 -0.034090977653473686 1104 58-59 -0.07001829459343512 1104 60-61 -0.050959137830368206 1104 62-63 -0.2325012049306281 1104 64-65 -0.20275244240913537 1104 66-67 -0.14199336505484084 1104 68-69 -0.030327712653253514 1104 70-71 -0.0013183461412040742 1104 72-73 -0.26104906009928186 1104 74-75 -0.1859555456184978 1104 76-77 -0.09718476383322638 1104 78-79 -0.13936793663373948 1104 80-81 -0.09931354346009158 1104 82-83 -0.10871953745299479 1104 84-85 -0.15641829932749474 1104 86-87 -0.02848126518204097 1104 88-89 -0.0038822675017158303 1104 90-91 -0.12837263527852016 1104 92-93 0.007451929487359621 1104 94-95 0.09071790625599974 1104 96-97 0.15084784030475618 1104 98-99 0.16697008875204133 1104 100-101 0.10127729613808611 1104 102-103 0.29591568791236256 1104 104-105 0.058805178811535797 1104 106-107 0.050486240617278355 1104 108-109 0.07374560953600451 1104 110-111 0.034782704973132184 1104 112-113 -0.08435822406141469 1104 114-115 -0.14713831876157712 1104 116-117 0.04493208951183192 1104 118-119 0.08261054196974271 1104 120-121 0.1420316208386332 1104 122-123 0.191653142557211 1104 124-125 0.25833391885167956 1105 1 0.37142895187031755 1105 2 0.41853830151030635 1105 3 0.34226064980610005 1105 4 0.37394418561373755 1105 5 0.43815470063465156 1105 6 0.38036491069309974 1105 7 0.22486977392957286 1105 8 0.36710875624063277 1105 9 0.47987626782304105 1105 10-11 0.17681399161605427 1105 12-13 0.3700093749185207 1105 14-15 0.4437133687360628 1105 16-17 0.3448005768284901 1105 18-19 0.24754338073098126 1105 20-21 0.10614104854339246 1105 22-23 0.21442490353489063 1105 24-25 0.32632422142733475 1105 26-27 0.2730952912493336 1105 28-29 0.3315581250884705 1105 30-31 0.27260487762604413 1105 32-33 0.27054382425595236 1105 34-35 0.312155702761693 1105 36-37 0.19884115611255382 1105 38-39 0.1296677050331816 1105 40-41 0.2724427841863388 1105 42-43 0.43665341188366824 1105 44-45 0.3455969821577405 1105 46-47 0.477549406403746 1105 48-49 0.4832355053940631 1105 50-51 0.5763972043983863 1105 52-53 0.8039741269701821 1105 54-55 0.6576396873773191 1105 56-57 0.6838522434290901 1105 58-59 0.5557816398321833 1105 60-61 0.7050039493689582 1105 62-63 0.7427391570293302 1105 64-65 0.5942623304101531 1105 66-67 0.7547721189278853 1105 68-69 0.8558390135259231 1105 70-71 0.7869978657304095 1105 72-73 0.8019926088350537 1105 74-75 0.709113499955695 1105 76-77 0.8163121426223974 1105 78-79 0.7492286256227629 1105 80-81 0.7539538261179075 1105 82-83 0.910435084014253 1105 84-85 0.8678973178614875 1105 86-87 0.9198404156459503 1105 88-89 0.8586551158681459 1105 90-91 0.7116039756410757 1105 92-93 0.7220195301685912 1105 94-95 0.7975559601039812 1105 96-97 1.0123551483968214 1105 98-99 1.0372230810808993 1105 100-101 1.1083252145497546 1105 102-103 1.003175196529284 1105 104-105 0.8921619631713789 1105 106-107 0.9071856458019028 1105 108-109 1.0471222785964436 1105 110-111 1.2147797315599576 1105 112-113 1.2448636576047036 1105 114-115 1.2406292718312883 1105 116-117 1.0709992328980427 1105 118-119 1.237441077330132 1105 120-121 1.1724281334497597 1105 122-123 1.3172413540359145 1105 124-125 1.1915296875785408 1106 1 0.37563891736403576 1106 2 0.344989631719983 1106 3 0.5315236195371718 1106 4 -0.12326742801549528 1106 5 0.284621665725048 1106 6 0.8181453053493577 1106 7 0.8769476197019337 1106 8 0.7279969070923329 1106 9 0.9547347323184177 1106 10-11 0.7862600238916251 1106 12-13 0.9235208745226693 1106 14-15 0.938630165278461 1106 16-17 0.8081233375301622 1106 18-19 0.7980530081328538 1106 20-21 0.8933411102862365 1106 22-23 0.8554936024041027 1106 24-25 0.9032737267377158 1106 26-27 0.8050198255639174 1106 28-29 0.7548980393455906 1106 30-31 0.6053141435335405 1106 32-33 0.7478091392191288 1106 34-35 0.6880084012724055 1106 36-37 0.8100355315389045 1106 38-39 0.7974034606111928 1106 40-41 0.7614348820985342 1106 42-43 0.8026814052911284 1106 44-45 0.7462320869462964 1106 46-47 0.9431445775160618 1106 48-49 0.8830328494560469 1106 50-51 1.0493803545778881 1106 52-53 1.0279182745953221 1106 54-55 1.0564990630775277 1106 56-57 1.1388689737473143 1106 58-59 1.1049642940772522 1106 60-61 1.1377440866969835 1106 62-63 1.0797131603056833 1106 64-65 1.1919268078615701 1106 66-67 1.2315152338542212 1106 68-69 1.1399226800548306 1106 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-0.9088933565568169 2207 92-93 -0.9054263560276397 2207 94-95 -0.9424319638215586 2207 96-97 -0.9570559384979909 2207 98-99 -1.2577921703756871 2207 100-101 -1.469537801389702 2207 102-103 -1.262234427765275 2207 104-105 -0.8573635337037544 2207 106-107 -0.7144892239352636 2207 108-109 -0.807790422450946 2207 110-111 -0.7139978906561666 2207 112-113 -0.8595131977085266 2207 114-115 -0.8787121731600251 2207 116-117 -0.956291612860177 2207 118-119 -1.0038001602991073 2207 120-121 -1.0642190238615576 2207 122-123 -1.0385662274294774 2207 124-125 -0.7797445307241695 2208 1 0.03836060919705275 2208 2 -0.07240673033010125 2208 3 0.007450487928448268 2208 4 -0.06650114107821636 2208 5 -0.15192096408651068 2208 6 0.40415355730669944 2208 7 0.32632599648376015 2208 8 0.2079043452140148 2208 9 0.16035092542148988 2208 10-11 0.21812287145307252 2208 12-13 0.15356520152919728 2208 14-15 0.28659546252615087 2208 16-17 0.38828910372168224 2208 18-19 0.2398167276935368 2208 20-21 0.21224904370711783 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-3.193866371818036 2216 110-111 -3.522542194453635 2216 112-113 -3.52512501205452 2216 114-115 -3.5633113292781715 2216 116-117 -3.735300051678742 2216 118-119 -3.7696229451092336 2216 120-121 -3.3252949732286474 2216 122-123 -2.9104016704674542 2216 124-125 -2.581432294437249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63111.0 3 5266.0 4 2030.0 5 3635.0 6 6599.0 7 8832.0 8 15804.0 9 8518.0 10 6461.0 11 5448.0 12 4376.0 13 3937.0 14 3883.0 15 4068.0 16 4155.0 17 4169.0 18 4147.0 19 4105.0 20 4066.0 21 4372.0 22 4881.0 23 5427.0 24 6696.0 25 7651.0 26 8111.0 27 7954.0 28 8708.0 29 10629.0 30 12696.0 31 16224.0 32 20872.0 33 26342.0 34 37804.0 35 56541.0 36 118373.0 37 80975.0 38 10432.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.47528548460238 9.750257657583377 5.541493177227192 9.232963680587048 2 11.348156589443454 40.18565848324561 38.307665452437575 10.158519474873362 3 44.45267758051184 22.041093228739573 15.418687901571213 18.087541289177377 4 13.03349027008601 24.24368177244665 43.09354780807277 19.629280149394567 5 17.676938985194163 34.16341746140471 23.334013910111175 24.82562964328995 6 28.574698071112792 42.06423037976768 12.376491121191414 16.984580427928115 7 42.50678776243408 29.95720704774732 11.278964275541169 16.257040914277436 8 41.75218945472328 27.291991510563225 13.50324281336905 17.452576221344447 9 55.767644419563844 17.66821164247668 7.632400036223235 18.931743901736244 10-11 40.348401649803655 30.44916810050301 12.028418773514229 17.174011476179107 12-13 29.641389984275822 32.67747326478361 17.62136841498654 20.05976833595403 14-15 27.872543611950373 30.235039392766875 17.74543299113354 24.146984004149207 16-17 25.373058146522983 34.086557063942244 20.367501173138823 20.172883616395954 18-19 24.584379553630967 27.540854045064993 24.164766318978508 23.71000008232553 20-21 26.961364628012085 27.185866352731974 20.196758020564918 25.656010998691027 22-23 31.738715227753584 21.672937127991503 21.750323127711596 24.838024516543317 24-25 28.92079460603117 23.77322609060748 25.921922465814323 21.38405683754703 26-27 23.37864304667002 27.507641949258936 23.80616410782455 25.307550896246493 28-29 22.451625970074048 29.300394039838697 22.282434188101742 25.965545801985513 30-31 21.11098304917304 32.31507627460504 22.8813112810676 23.69262939515432 32-33 26.409865891708996 33.22971293087125 19.14982423499 21.210596942429756 34-35 25.377586050761924 28.934625295342848 20.561377800097144 25.126410853798088 36-37 22.462850603857774 34.90446122055833 20.906321777572877 21.726366398011017 38-39 19.81106290493871 38.78429887460999 19.167112596629593 22.237525623821718 40-41 20.984531032609144 40.000082325531615 17.837308284418246 21.178078357440995 42-43 20.27669611176514 36.029768912232754 16.44180819797644 27.25172677802567 44-45 20.69161679111543 33.81117816068298 18.82241559574871 26.67478945245289 46-47 23.2477422222954 32.087116877557236 20.644032633840734 24.02110826630663 48-49 17.862582222624702 32.891108019330034 23.306851953996492 25.93945780404877 50-51 21.35911220146704 34.163037482814545 22.34866509150483 22.129185224213586 52-53 23.288246383851025 32.15915171772222 22.456840840049725 22.095761058377033 54-55 17.616017255431426 31.95547835250146 24.4268084861158 26.001695905951312 56-57 16.698856161530344 32.55510495751152 21.73186068452484 29.014178196433306 58-59 17.16026311239905 31.57052416665981 26.430529600144894 24.838683120796254 60-61 19.132057670728887 31.83934323929829 25.42757858184276 23.601020508130063 62-63 20.961891511414436 29.254871613333442 25.53943804592118 24.243798829330938 64-65 23.6913058332956 26.43774209349903 27.83936311657947 22.0315889566259 66-67 23.598652493479808 26.470665718274983 26.192569614584155 23.738112173661055 68-69 19.86041633048249 28.26540491943106 25.660293163578164 26.213885586508283 70-71 18.726187760869333 29.903190665067036 23.45829260841748 27.91232896564615 72-73 18.60682034660724 27.827946083318444 24.75275564362565 28.812477926448665 74-75 19.663734504880715 26.333222457878303 28.313584329016876 25.689458708224105 76-77 19.232773545777636 24.532328081225863 28.656682750371083 27.578215622625418 78-79 18.22908862789522 25.612057226877333 29.843315984371117 26.315538160856327 80-81 16.466694657808294 22.146014967028083 33.00517836120098 28.38211201396264 82-83 17.341146581552973 21.808298255767525 29.90683211916795 30.94372304351155 84-85 18.216678619921893 23.32350449132243 28.75170961741624 29.70810727133944 86-87 19.181727172137624 24.746841904209916 25.810188234364297 30.26124268928816 88-89 18.57348592827906 27.09466041201953 25.304724662956367 29.02712899674505 90-91 18.322714703105074 29.43000224748886 24.915266376825674 27.33201667258039 92-93 17.488927697183325 30.168947820409265 24.619581282106385 27.722543200301025 94-95 15.968115641318256 28.760722395954268 25.8784172105056 29.392744752221876 96-97 17.802795472115672 28.31360267148139 26.32291761862067 27.56068423778226 98-99 18.722908884938967 29.150677849028238 26.724137221308837 25.40227604472396 100-101 16.936504983213656 31.09680096288893 26.74074940025258 25.225944653644834 102-103 16.62333599519219 33.59688480188361 26.08179864821477 23.69798055470943 104-105 15.54934999394903 35.5973296692712 25.054808628767557 23.798511708012228 106-107 14.962862463508728 36.64966896467694 23.802600533147164 24.584868038667164 108-109 15.805761140702565 36.56068626563156 23.159242275806996 24.474310317858876 110-111 15.546765018235106 36.16585301599585 23.959117140998938 24.32826482477011 112-113 16.545825014921686 34.94770206022187 24.32749500895301 24.17897791590343 114-115 18.01322060662644 34.61645174330387 23.87966926254241 23.490658387527283 116-117 19.38107665330249 35.430439042060115 21.58040323045386 23.608081074183538 118-119 19.40578740624547 34.64114588139145 21.71579030620988 24.237276406153196 120-121 19.398224872043752 34.356077726268715 21.531309249485254 24.71438815220228 122-123 19.949861068230938 34.495832046928065 21.148996603890087 24.40531028095091 124-125 19.27670176533163 36.6254940262947 21.064645749413856 23.033158458959814 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 62.0 1 107.5 2 181.0 3 211.5 4 205.0 5 181.5 6 161.0 7 154.0 8 164.5 9 180.0 10 193.5 11 205.0 12 232.5 13 289.0 14 326.5 15 378.5 16 482.5 17 632.0 18 796.5 19 988.0 20 1242.5 21 1541.5 22 1913.0 23 2380.5 24 2969.0 25 3670.5 26 4415.5 27 5242.0 28 6173.5 29 6962.5 30 7690.0 31 8173.0 32 8471.5 33 8957.5 34 9191.0 35 8965.5 36 8736.5 37 8662.0 38 8226.0 39 7484.5 40 6745.5 41 6159.5 42 5665.0 43 5332.5 44 5299.5 45 5596.5 46 6925.5 47 10469.0 48 18476.5 49 33978.0 50 52745.0 51 61506.0 52 56177.5 53 44172.0 54 33320.0 55 27528.5 56 23426.0 57 18352.0 58 13587.0 59 9956.5 60 7397.0 61 5537.0 62 4191.0 63 2960.0 64 2056.0 65 1739.0 66 1665.5 67 1557.5 68 1262.5 69 993.5 70 862.5 71 748.0 72 708.0 73 739.0 74 693.0 75 527.0 76 352.0 77 231.5 78 173.5 79 138.0 80 80.5 81 40.0 82 28.0 83 21.0 84 15.0 85 9.5 86 6.0 87 6.5 88 6.0 89 3.0 90 1.5 91 1.0 92 2.0 93 2.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15147897817550157 2 0.016629757386658325 3 0.007079995719072356 4 0.015477199944018638 5 0.0027990680749820944 6 0.0 7 3.2930212646848164E-4 8 3.2930212646848164E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010702319110225654 28-29 0.007573948908775078 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.586042529369633E-4 58-59 0.0 60-61 8.232553161712041E-5 62-63 0.0 64-65 0.002058138290428011 66-67 1.6465106323424082E-4 68-69 0.006421391466135393 70-71 0.01984045311972602 72-73 8.232553161712041E-5 74-75 0.009385110604351727 76-77 2.4697659485136124E-4 78-79 0.0013172085058739266 80-81 0.0016465106323424084 82-83 8.232553161712041E-5 84-85 0.01951115099325754 86-87 0.0027990680749820944 88-89 0.019181848866789055 90-91 8.232553161712041E-5 92-93 0.014242316969761831 94-95 0.014489293564613192 96-97 0.0068330191242209955 98-99 0.0032106957330676963 100-101 9.87906379405445E-4 102-103 0.0 104-105 7.409297845540838E-4 106-107 0.0013172085058739266 108-109 0.0 110-111 0.0 112-113 0.0012348829742568062 114-115 0.00584511274481555 116-117 0.0 118-119 0.0726111188863002 120-121 0.0013995340374910472 122-123 0.0053511595551128275 124-125 0.2439305501815278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 607345.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.82570199183702 #Duplication Level Percentage of deduplicated Percentage of total 1 84.61000758358065 43.003630309701414 2 7.770379398783088 7.89869975372118 3 2.555330811706513 3.896294469790628 4 1.265894694834947 2.57359946050914 5 0.7633562469096278 1.9399058559517952 6 0.5431438054847195 1.656339911776719 7 0.38203532976745197 1.3592049674779592 8 0.2837055305498015 1.1535626199328182 9 0.21660062384396703 0.9907990882865512 >10 1.3915565929712614 13.53966730895377 >50 0.12742999527273471 4.592059484416702 >100 0.07697547835100174 7.5036240261607485 >500 0.006791953972147207 2.447838091737894 >1k 0.006145101212895092 5.42680000822934 >5k 6.46852759252115E-4 2.0179746433533268 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 6849 1.1276951320913153 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 5427 0.893561320172225 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3878 0.6385168232223859 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3024 0.4979048152203443 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 2217 0.3650314071903119 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 2204 0.36289094336826677 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 2102 0.3460965349183743 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2043 0.33638212218755403 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 1963 0.3232100371288148 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1920 0.3161300414097424 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1497 0.24648264166165854 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1438 0.23676822893083832 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1283 0.211247314129531 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1280 0.21075336093982827 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1216 0.20021569289283683 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1215 0.20005104182960262 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1213 0.19972173970313414 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1198 0.19725197375462053 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1182 0.19461755674287268 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1120 0.18440919082234974 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1020 0.16794408449892564 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 994 0.1636631568548354 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 986 0.16234594834896146 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 938 0.1544426973137179 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 917 0.15098502498579885 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 906 0.1491738632902222 No Hit CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT 870 0.14324642501378954 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 781 0.1285924803859421 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCCTGTC 724 0.11920736978159037 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 676 0.11130411874634681 No Hit CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 663 0.10916365492430168 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 617 0.1015897060155266 No Hit GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC 608 0.10010784644641842 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6465106323424085E-4 0.0 0.0 0.0 6 0.0 1.6465106323424085E-4 0.0 0.0 0.0 7 0.0 1.6465106323424085E-4 0.0 0.0 0.0 8 0.0 1.6465106323424085E-4 0.0 0.0 0.0 9 0.0 1.6465106323424085E-4 0.0 0.0 0.0 10-11 0.0 1.6465106323424085E-4 0.0 1.6465106323424085E-4 0.0 12-13 0.0 1.6465106323424085E-4 0.0 1.6465106323424085E-4 0.0 14-15 0.0 1.6465106323424085E-4 0.0 2.469765948513613E-4 0.0 16-17 0.0 1.6465106323424085E-4 0.0 3.293021264684817E-4 0.0 18-19 0.0 1.6465106323424085E-4 0.0 4.939531897027226E-4 0.0 20-21 8.232553161712042E-5 1.6465106323424085E-4 0.0 0.00197581275881089 0.0 22-23 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.008644180819797644 0.0 24-25 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.018770221208703454 0.0 26-27 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.03704648922770419 0.0 28-29 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.08076134651639513 0.0 30-31 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.20869522264940027 0.0 32-33 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.4522964707044596 0.0 34-35 1.6465106323424085E-4 1.6465106323424085E-4 0.0 0.7461162930459624 0.0 36-37 1.6465106323424085E-4 1.6465106323424085E-4 0.0 1.1597197638903753 0.0 38-39 1.6465106323424085E-4 1.6465106323424085E-4 0.0 1.7682700936041296 0.0 40-41 1.6465106323424085E-4 1.6465106323424085E-4 0.0 2.526982192987511 0.0 42-43 1.6465106323424085E-4 1.6465106323424085E-4 0.0 3.6908182334587423 0.0 44-45 1.6465106323424085E-4 1.6465106323424085E-4 0.0 5.2271773044974434 0.0 46-47 1.6465106323424085E-4 1.6465106323424085E-4 0.0 6.917732096255012 0.0 48-49 1.6465106323424085E-4 1.6465106323424085E-4 0.0 7.673068848842091 0.0 50-51 1.6465106323424085E-4 1.6465106323424085E-4 0.0 8.635783615572699 0.0 52-53 3.293021264684817E-4 1.6465106323424085E-4 0.0 9.388979904337733 0.0 54-55 3.293021264684817E-4 1.6465106323424085E-4 0.0 10.119042718718354 0.0 56-57 3.293021264684817E-4 1.6465106323424085E-4 0.0 11.367097778033902 0.0 58-59 3.293021264684817E-4 1.6465106323424085E-4 0.0 12.620997950094264 0.0 60-61 3.293021264684817E-4 1.6465106323424085E-4 0.0 14.183207238060739 0.0 62-63 3.293021264684817E-4 1.6465106323424085E-4 0.0 15.84116111929793 0.0 64-65 3.293021264684817E-4 1.6465106323424085E-4 0.0 17.173105895331318 0.0 66-67 3.293021264684817E-4 1.6465106323424085E-4 0.0 18.368801916538374 0.0 68-69 3.293021264684817E-4 1.6465106323424085E-4 0.0 19.44372638286312 0.0 70-71 3.293021264684817E-4 1.6465106323424085E-4 0.0 20.675563312450087 0.0 72-73 3.293021264684817E-4 1.6465106323424085E-4 0.0 21.8989207122805 0.0 74-75 3.293021264684817E-4 1.6465106323424085E-4 0.0 23.14623484181149 0.0 76-77 3.293021264684817E-4 1.6465106323424085E-4 0.0 24.576723279190574 0.0 78-79 3.293021264684817E-4 1.6465106323424085E-4 0.0 26.470539808510814 0.0 80-81 3.293021264684817E-4 1.6465106323424085E-4 0.0 28.239468506367878 0.0 82-83 3.293021264684817E-4 3.293021264684817E-4 0.0 29.578163975993874 0.0 84-85 3.293021264684817E-4 3.293021264684817E-4 0.0 30.665107969934716 0.0 86-87 3.293021264684817E-4 4.939531897027225E-4 0.0 31.777161251018782 0.0 88-89 3.293021264684817E-4 4.939531897027225E-4 0.0 33.456190468349945 0.0 90-91 3.293021264684817E-4 4.939531897027225E-4 0.0 35.70079608789074 0.0 92-93 3.293021264684817E-4 4.939531897027225E-4 0.0 38.57971992854144 0.0 94-95 3.293021264684817E-4 4.939531897027225E-4 0.0 42.3066790703801 0.0 96-97 3.293021264684817E-4 4.939531897027225E-4 0.0 46.322683153726466 0.0 98-99 3.293021264684817E-4 4.939531897027225E-4 0.0 48.94433970807367 0.0 100-101 3.293021264684817E-4 4.939531897027225E-4 0.0 50.478229013163855 0.0 102-103 3.293021264684817E-4 4.939531897027225E-4 0.0 51.827626801900074 0.0 104-105 3.293021264684817E-4 4.939531897027225E-4 0.0 53.14738739925413 0.0 106-107 3.293021264684817E-4 4.939531897027225E-4 0.0 54.49456239863669 0.0 108-109 3.293021264684817E-4 4.939531897027225E-4 0.0 55.70433608575027 0.0 110-111 3.293021264684817E-4 4.939531897027225E-4 0.0 56.60094345059233 0.0 112-113 3.293021264684817E-4 4.939531897027225E-4 0.0 57.46651409001474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCGGT 185 0.0 77.19149 2 GAGTAGT 1135 0.0 75.62201 1 AGTAGTA 905 0.0 75.609985 2 AGTCCGT 150 0.0 75.368904 2 AGTTAGT 420 0.0 75.08557 2 AGTCGTA 240 0.0 74.37721 2 GAGTTAG 710 0.0 73.876434 1 GTCCGTG 155 0.0 72.93165 3 TGACCGA 25 0.0020561363 71.40212 2 AGTCAGA 445 0.0 70.86727 2 GAGTCAA 335 0.0 69.390724 1 GAGTCAG 2955 0.0 68.98429 1 AGTAATC 95 0.0 68.89678 2 GAGTAAT 340 0.0 68.37027 1 AGTTGTA 210 0.0 68.00202 2 GAGTCGG 415 0.0 67.50429 1 GTTAGTG 460 0.0 67.25733 3 AGTTGCG 45 3.985904E-6 66.113075 2 AGTCGAT 45 3.985904E-6 66.113075 2 AGTCAGT 2265 0.0 64.09904 2 >>END_MODULE