FastQCFastQC Report
Thu 26 May 2016
SRR935943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607345
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC55980.9217166519852802No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG44210.7279223505585787No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG43790.7210070059027406No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA25150.4140974240341157No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT23660.3895644156122138No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT23430.3857774411578263No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT21210.3492249051198248No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC17390.2863281989643448No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT16950.27908355218203823No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC16300.26838123307181255No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG16050.26426495649095655No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG14280.23512171829849593No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG12550.20663708435897224No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC12390.20400266734722441No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG12350.20334406309428743No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG11930.19642871843844933No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC11690.19247709292082754No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA11550.19017197803554817No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT11520.18967802484584545No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG10870.17897570573561977No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG10750.1769998929768089No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT9810.16152269303279027No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC9610.15822967176810546No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC9270.15263153561814125No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT9080.14950316541669068No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA8660.14258782076085255No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA7860.1294157357021133No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA7700.12678131869036544No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCCTGTC7320.1205245782874643No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA7150.11772551021248219No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG7020.11558504639043705No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA7020.11558504639043705No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT6850.11278597831545498No Hit
GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC6720.11064551449340984No Hit
ACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGC6680.10998691024047288No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT6520.10735249322872502No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT6500.10702319110225654No Hit
CAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTT6360.10471807621697718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT509.094947E-1295.363183
GTCCGTT509.094947E-1295.363183
TATTCGG1000.089.4029852
AGTTAAC401.8764695E-889.402982
CGAGGGT208.4830343E-489.241046
GAGTAAT3900.088.8293461
TAAGGTT1900.087.834514
GTAAGGT1900.087.834513
AGTCGGT1900.087.834512
AGTCGCG552.0008883E-1186.69382
AGTACGT1450.086.320122
GTATTCG1050.085.328681
GTCGTTG358.8624984E-785.145693
AGTAAGG3800.084.697562
GAGTCGG5150.083.5061341
AGTCCGT1850.080.543232
GTGATCG604.3655746E-1179.640111
TGATCGG604.3655746E-1179.4693152
GTCTAGT304.2265405E-579.4693153
AGTAATC604.3655746E-1179.4693152
Produced by FastQC (version 0.11.5)