FastQCFastQC Report
Thu 26 May 2016
SRR935942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518706
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC202901.3360057838712693No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT202471.3331744261232918No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT179661.1829807744224359No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG163301.0752574889412434No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT148590.978398715748802No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA121240.798311193871625No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT95720.6302734038056081No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC67990.4476837518255673No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC59710.3931636537947437No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG52380.3448988810210798No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG48270.3178363685927362No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG47630.3136222547352812No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC42900.2824773195075281No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA42790.28175301868827807No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG40670.2677937665354585No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC40160.26443564455529905No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA39920.26285535185875347No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA38770.255283116021139No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA36010.2371097500108645No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT34040.22413818079338593No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC31980.2105740018147028No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT31140.20504297737679317No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG29850.19654890413286047No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT28990.19088618863690537No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG26450.17416142426513098No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA25650.16889378194331225No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG24750.1629676843312662No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT23880.1572391233062884No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA22530.1483499768882193No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA22520.14828413135919657No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA22480.14802074924310565No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT22030.1450577004370826No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC21970.1446626272629462No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA21520.1416995784569232No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA21420.14104112316669584No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT20310.13373226944517239No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA19830.13057168405208117No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18750.12346036691762592No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18670.12293360268544405No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA18170.11964132623430736No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG17160.11299092780301125No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG16160.10640637490073786No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG16150.10634052937171515No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC15690.10331163503666937No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA15590.10265317974644204No Hit
GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG15460.1017971878691465No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA15250.10041443175966909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT35450.095.1323241
AGTAGTA29750.094.992772
AGTCGTA6150.093.838392
AGTTTCG1400.093.492882
GAGTAAG14950.093.261541
GAGTTAG17600.089.03711
GAGTCGT9850.088.921961
GAGTCAG79950.086.823131
GAGTACG8550.085.7169951
GAGTCGG11750.084.177921
AGTAAGG7950.083.069152
GAGTCCG8550.082.929451
GAGTCGA5000.082.225591
AGTTAGG4750.081.4148562
GAGTTCG5600.080.863681
GAGTAGG15150.080.62491
GAGTGGT12750.080.379671
AGTCGAG2300.080.189552
AGTCCGT4200.079.327292
AGTACGT1500.079.3272862
Produced by FastQC (version 0.11.5)