##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1518706 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.360680737417248 33.0 31.0 34.0 30.0 34.0 2 31.122851295774165 31.0 31.0 34.0 28.0 34.0 3 31.412919287867435 33.0 31.0 34.0 28.0 34.0 4 34.67383153816473 37.0 35.0 37.0 32.0 37.0 5 34.78414979594471 37.0 35.0 37.0 32.0 37.0 6 34.980176545032414 37.0 35.0 37.0 32.0 37.0 7 34.97740971590288 37.0 35.0 37.0 32.0 37.0 8 34.911250103706706 37.0 35.0 37.0 32.0 37.0 9 36.60305615438406 39.0 37.0 39.0 33.0 39.0 10-11 36.28456198895638 39.0 37.0 39.0 32.0 39.0 12-13 36.068386837215364 39.0 37.0 39.0 31.5 39.0 14-15 37.550253966205446 40.0 38.0 41.0 32.0 41.0 16-17 37.67187559672511 40.0 38.0 41.0 32.0 41.0 18-19 37.67479419979904 40.0 38.0 41.0 32.0 41.0 20-21 37.778916393297976 40.0 38.0 41.0 32.0 41.0 22-23 37.6464816100022 40.0 38.0 41.0 32.0 41.0 24-25 37.55877174383983 40.0 38.0 41.0 32.0 41.0 26-27 37.52484417655557 40.0 38.0 41.0 31.5 41.0 28-29 37.41762000018437 40.0 37.0 41.0 31.0 41.0 30-31 37.34571075639393 40.0 37.0 41.0 31.0 41.0 32-33 37.25351977275391 40.0 37.0 41.0 31.0 41.0 34-35 36.960464039781236 40.0 36.0 41.0 30.0 41.0 36-37 36.95118739242487 40.0 36.0 41.0 30.5 41.0 38-39 37.07039051666353 40.0 36.0 41.0 30.5 41.0 40-41 37.03087727315227 40.0 36.0 41.0 30.0 41.0 42-43 36.8589384647193 40.0 35.5 41.0 30.0 41.0 44-45 36.642883151841104 40.0 35.0 41.0 30.0 41.0 46-47 36.45187185669906 39.5 35.0 41.0 30.0 41.0 48-49 36.26352236706775 39.0 35.0 41.0 30.0 41.0 50-51 35.9929729651427 39.0 35.0 41.0 30.0 41.0 52-53 35.70554340339737 38.5 35.0 40.5 29.0 41.0 54-55 35.423847341091694 38.0 35.0 40.0 29.0 41.0 56-57 35.11960741578686 37.0 35.0 40.0 28.0 41.0 58-59 34.70765967870015 37.0 34.0 39.5 28.0 41.0 60-61 34.382290252359574 36.0 34.0 39.0 28.0 41.0 62-63 33.91291434945276 36.0 34.0 39.0 26.5 41.0 64-65 33.42225980538696 35.0 33.5 37.5 26.0 40.0 66-67 33.08064497012589 35.0 33.0 37.0 26.0 39.0 68-69 32.88485493571501 35.0 33.0 36.0 26.5 39.0 70-71 32.75398694678233 35.0 33.5 36.0 27.0 38.0 72-73 32.52470524248933 35.0 33.5 35.5 26.5 37.0 74-75 32.21978414518676 35.0 33.0 35.0 26.0 37.0 76-77 32.05108329064348 35.0 33.0 35.0 26.0 36.0 78-79 31.88040081490427 35.0 33.0 35.0 25.0 36.0 80-81 31.750957723219635 35.0 33.0 35.0 25.0 35.5 82-83 31.69383409297125 35.0 33.0 35.0 25.0 35.0 84-85 31.47095092796104 35.0 33.0 35.0 25.0 35.0 86-87 31.336506868347133 35.0 33.0 35.0 24.0 35.0 88-89 31.257907718807985 35.0 33.0 35.0 24.0 35.0 90-91 31.233035557902582 35.0 33.0 35.0 24.0 35.0 92-93 31.145266759991728 35.0 32.5 35.0 23.0 35.0 94-95 30.958495258463458 35.0 32.0 35.0 21.5 35.0 96-97 30.82984560540355 35.0 32.0 35.0 20.0 35.0 98-99 30.66419109426051 35.0 32.0 35.0 19.0 35.0 100-101 30.546001661941155 35.0 32.0 35.0 19.0 35.0 102-103 29.82801147819262 34.0 30.0 35.0 18.0 35.0 104-105 30.423125674093605 35.0 31.5 35.0 18.5 35.0 106-107 30.47949240998587 35.0 32.0 35.0 18.0 35.0 108-109 30.35791061601126 35.0 31.5 35.0 17.0 35.0 110-111 30.245467852237365 35.0 31.0 35.0 16.0 35.0 112-113 30.10689132722199 35.0 31.0 35.0 11.5 35.0 114-115 29.873558805983514 35.0 31.0 35.0 4.5 35.0 116-117 29.709022681150927 35.0 31.0 35.0 2.0 35.0 118-119 29.498755848729118 35.0 31.0 35.0 2.0 35.0 120-121 29.249821887843993 35.0 31.0 35.0 2.0 35.0 122-123 28.810655584425163 34.5 30.5 35.0 2.0 35.0 124-125 25.88740315768819 31.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2094001074201195 1101 2 0.42834083611087337 1101 3 0.2873927641990903 1101 4 0.2851163790143829 1101 5 0.3048904746916392 1101 6 0.21972521401374223 1101 7 0.28745539969850853 1101 8 0.19149994393994518 1101 9 0.25754409740060424 1101 10-11 0.458716646561804 1101 12-13 0.26548992697097873 1101 14-15 0.35522175147245605 1101 16-17 0.2735293500180518 1101 18-19 0.43150483550126495 1101 20-21 0.30183678308042516 1101 22-23 0.4288449601852662 1101 24-25 0.42377917986534896 1101 26-27 0.38454924279849934 1101 28-29 0.3605843920032896 1101 30-31 0.33039865659984 1101 32-33 0.3524912146368493 1101 34-35 0.06985676468889324 1101 36-37 0.04085058127190422 1101 38-39 0.3277453109229569 1101 40-41 0.30252258083958594 1101 42-43 0.17034548193934995 1101 44-45 0.31974358216599086 1101 46-47 0.26436481145779567 1101 48-49 0.22048489124527038 1101 50-51 0.34141045581699814 1101 52-53 0.3713373550964221 1101 54-55 0.28081216282342325 1101 56-57 0.1910198727063417 1101 58-59 0.14823285435453215 1101 60-61 0.28750078259916023 1101 62-63 0.329742928109269 1101 64-65 0.3726396679442274 1101 66-67 0.2727248157114559 1101 68-69 0.30540002807887845 1101 70-71 0.20211972064181083 1101 72-73 0.4180573852591323 1101 74-75 0.4499604371005006 1101 76-77 0.5041030936808397 1101 78-79 0.6125025685267254 1101 80-81 0.5581905121355604 1101 82-83 0.4463042634522232 1101 84-85 0.3484052371358217 1101 86-87 0.35932283337951887 1101 88-89 0.38189078802966847 1101 90-91 0.22111366375469288 1101 92-93 -0.09788878463746542 1101 94-95 0.03809460828355071 1101 96-97 -0.00841611562572453 1101 98-99 -0.07592702855792766 1101 100-101 0.09281041428251413 1101 102-103 0.11835154505936529 1101 104-105 -0.07277038043444861 1101 106-107 0.06025043200430602 1101 108-109 0.11633536523175536 1101 110-111 -0.020797044645018303 1101 112-113 0.06407322035878948 1101 114-115 0.03544883659913367 1101 116-117 -0.0681889487410885 1101 118-119 0.0063469204740371765 1101 120-121 -0.12427522135842395 1101 122-123 -0.1035060940649366 1101 124-125 0.05933534940495733 1102 1 0.32288447034345324 1102 2 0.292961651428179 1102 3 0.33896743011811026 1102 4 0.43875364453400323 1102 5 0.4451871169198469 1102 6 0.34068800142014055 1102 7 0.3853445371557953 1102 8 0.32315262329098715 1102 9 0.3823884377545994 1102 10-11 0.5225589596390847 1102 12-13 0.6514456439729344 1102 14-15 0.6133882726119779 1102 16-17 0.708439311652505 1102 18-19 0.7137814940735865 1102 20-21 0.6523594567541551 1102 22-23 0.7331247406937962 1102 24-25 0.6908026503439402 1102 26-27 0.696999449781238 1102 28-29 0.74218723462063 1102 30-31 0.6499162370176705 1102 32-33 0.7302446796013342 1102 34-35 0.7602646798113994 1102 36-37 0.6311153396482609 1102 38-39 0.5994165959879112 1102 40-41 0.61326365015519 1102 42-43 0.5167434227204808 1102 44-45 0.5784337678850591 1102 46-47 0.6135736605964723 1102 48-49 0.68129887957123 1102 50-51 0.6444690906285686 1102 52-53 0.6494151377056667 1102 54-55 0.6739489159864576 1102 56-57 0.7857404430950439 1102 58-59 0.607193382495673 1102 60-61 0.5713283836395036 1102 62-63 0.7272591352482252 1102 64-65 0.5933944817063832 1102 66-67 0.618034741906456 1102 68-69 0.644471444409703 1102 70-71 0.6987634643539309 1102 72-73 0.6812668009627743 1102 74-75 0.6415457814869114 1102 76-77 0.7370743772302859 1102 78-79 0.6674413014029987 1102 80-81 0.6439760264914582 1102 82-83 0.6753275450758203 1102 84-85 0.7567294288772644 1102 86-87 0.5996074681607801 1102 88-89 0.6114514903370818 1102 90-91 0.7083618716723805 1102 92-93 0.7451151288576199 1102 94-95 0.6738213094648238 1102 96-97 0.741941834528216 1102 98-99 0.6984572400544877 1102 100-101 0.75908802412442 1102 102-103 0.7960829769415838 1102 104-105 0.704426428615669 1102 106-107 0.521478852115731 1102 108-109 0.770750899837104 1102 110-111 0.7693793482479734 1102 112-113 0.6726172766142646 1102 114-115 0.5793276616413046 1102 116-117 0.6552148477105355 1102 118-119 0.5581239643338591 1102 120-121 0.45303233598879444 1102 122-123 0.4322586112688178 1102 124-125 0.29522304363707264 1103 1 0.07744335729273999 1103 2 0.12541745674113614 1103 3 -0.023787859930116895 1103 4 -0.08652073146848238 1103 5 -0.007536649363174774 1103 6 -0.12016864524381532 1103 7 -0.16807663415470842 1103 8 -0.07189480132677772 1103 9 -0.15399994830214325 1103 10-11 0.06927841695692649 1103 12-13 -0.051654237768111955 1103 14-15 -0.015623812394601089 1103 16-17 0.022891671407172964 1103 18-19 0.13316356641148275 1103 20-21 0.07761480283865296 1103 22-23 0.2000505639114607 1103 24-25 0.2556166681984422 1103 26-27 0.18467740977403224 1103 28-29 0.1722462340907498 1103 30-31 0.12239334956271364 1103 32-33 0.2157670362807238 1103 34-35 0.27443120974956514 1103 36-37 0.2724160670439062 1103 38-39 0.14490796236740522 1103 40-41 0.28029119041389094 1103 42-43 0.19379316230872234 1103 44-45 0.22854866465728207 1103 46-47 0.2564102301365878 1103 48-49 0.08282521796397191 1103 50-51 -0.04450740910380091 1103 52-53 0.1514082358301181 1103 54-55 0.2112411671114387 1103 56-57 0.12025535726092329 1103 58-59 0.16463044927925097 1103 60-61 0.21477126564325033 1103 62-63 -0.06817697936702416 1103 64-65 -0.018707201264589912 1103 66-67 0.0496616362686666 1103 68-69 0.11684713130232893 1103 70-71 0.04252985496351158 1103 72-73 -0.030366172214229437 1103 74-75 0.04222025647186456 1103 76-77 0.015864132723855562 1103 78-79 0.033259982371912855 1103 80-81 0.013542413335397185 1103 82-83 0.06383811441330423 1103 84-85 0.07838334617131437 1103 86-87 0.134342560046818 1103 88-89 0.02703351892185779 1103 90-91 -0.06608350372133032 1103 92-93 -0.030605744160595805 1103 94-95 -0.06275262389594616 1103 96-97 -0.12418437350173761 1103 98-99 0.07556577289936328 1103 100-101 0.0407566991310766 1103 102-103 0.08810244797710709 1103 104-105 0.010824246697723083 1103 106-107 -0.013359570655978814 1103 108-109 -0.05169503412616905 1103 110-111 -0.04703080677973759 1103 112-113 0.003707702122330403 1103 114-115 -0.11240907277993628 1103 116-117 0.02549524873299447 1103 118-119 -0.08917230795400855 1103 120-121 -0.14004077386292124 1103 122-123 0.004831635497108522 1103 124-125 -0.37942879179740174 1104 1 0.17162882871252805 1104 2 0.08060193470226551 1104 3 0.20194465455244526 1104 4 0.18159290127866967 1104 5 0.1966384574013773 1104 6 0.2442044651419124 1104 7 0.2520271414182531 1104 8 0.09220285006814777 1104 9 0.17571844272490722 1104 10-11 0.35510034271847246 1104 12-13 0.29070172147857676 1104 14-15 0.49976318490886484 1104 16-17 0.4885716530002284 1104 18-19 0.3470884377134311 1104 20-21 0.42275900486772144 1104 22-23 0.4165628359213045 1104 24-25 0.18993679412254494 1104 26-27 0.44382206767589594 1104 28-29 0.45245931161922925 1104 30-31 0.46811233838522526 1104 32-33 0.48857261083097825 1104 34-35 0.499407340937708 1104 36-37 0.41667776813757484 1104 38-39 0.33575631584945853 1104 40-41 0.45967885862450686 1104 42-43 0.37099414630586125 1104 44-45 0.20881023242964858 1104 46-47 0.34191207728157735 1104 48-49 0.45847816974036704 1104 50-51 0.4370716740757814 1104 52-53 0.28822555555607465 1104 54-55 0.3856044504277847 1104 56-57 0.32702689617568836 1104 58-59 0.2530866532350231 1104 60-61 0.3278007339493385 1104 62-63 0.23667580812313815 1104 64-65 0.23325455702821074 1104 66-67 0.25266598856373435 1104 68-69 0.35509722484226813 1104 70-71 0.32994518519174676 1104 72-73 0.2744313514502963 1104 74-75 0.3522805305976391 1104 76-77 0.4173492673998638 1104 78-79 0.39367173012803036 1104 80-81 0.356098569553545 1104 82-83 0.41451579513891446 1104 84-85 0.3113357461695898 1104 86-87 0.50116727695411 1104 88-89 0.5784942472001653 1104 90-91 0.462278128131274 1104 92-93 0.4931009317955528 1104 94-95 0.5284116150330149 1104 96-97 0.3712353950352991 1104 98-99 0.4906477439901451 1104 100-101 0.5487711365367325 1104 102-103 0.5618780424395453 1104 104-105 0.49388771193311953 1104 106-107 0.5833121278142706 1104 108-109 0.6041762635200669 1104 110-111 0.5223760424940949 1104 112-113 0.5958130388446072 1104 114-115 0.5346648474251232 1104 116-117 0.4433186926369892 1104 118-119 0.42143899167054144 1104 120-121 0.4802967440386894 1104 122-123 0.5446974970752017 1104 124-125 0.7508624507140311 1105 1 0.24881663987239477 1105 2 0.24113475472791635 1105 3 0.16535298152832922 1105 4 0.32038574130169906 1105 5 0.3035475316806284 1105 6 0.38394042488107516 1105 7 0.22975854042420707 1105 8 0.3880004898783156 1105 9 0.3948215539598152 1105 10-11 0.41304743072981154 1105 12-13 0.3950246479975519 1105 14-15 0.5050028312753554 1105 16-17 0.4684121312743841 1105 18-19 0.44685561997438583 1105 20-21 0.34384428891576135 1105 22-23 0.35221689786329335 1105 24-25 0.4997773971204964 1105 26-27 0.5641200328072813 1105 28-29 0.4588438184301893 1105 30-31 0.43348402566942923 1105 32-33 0.4958586627618118 1105 34-35 0.5530335811598874 1105 36-37 0.5605154775436176 1105 38-39 0.4667911283376327 1105 40-41 0.3864276420478845 1105 42-43 0.3508157081387253 1105 44-45 0.41509776649483854 1105 46-47 0.3540236926001512 1105 48-49 0.37975854258134945 1105 50-51 0.3562652100575576 1105 52-53 0.33320001836032276 1105 54-55 0.43690542348120687 1105 56-57 0.36685005863050435 1105 58-59 0.4362608944466757 1105 60-61 0.4607328031377804 1105 62-63 0.38377317374884967 1105 64-65 0.31200422183302123 1105 66-67 0.4542342862711024 1105 68-69 0.47733443701613965 1105 70-71 0.39941051509655523 1105 72-73 0.3615465525777992 1105 74-75 0.3533272749949461 1105 76-77 0.43113477990031157 1105 78-79 0.45965290099338674 1105 80-81 0.4440745726965858 1105 82-83 0.3030389199687171 1105 84-85 0.3525879314343072 1105 86-87 0.41205971723739765 1105 88-89 0.5734681903219574 1105 90-91 0.5204791329892657 1105 92-93 0.5910664789276332 1105 94-95 0.5516891275417706 1105 96-97 0.363563842660664 1105 98-99 0.41755451087930595 1105 100-101 0.5174203571169897 1105 102-103 0.5409162349423973 1105 104-105 0.38163744533602184 1105 106-107 0.36652534520991153 1105 108-109 0.49790629472180115 1105 110-111 0.4899038922767609 1105 112-113 0.5614113076784193 1105 114-115 0.6256041838529889 1105 116-117 0.49254914686024165 1105 118-119 0.32497595044725003 1105 120-121 0.31124248295438406 1105 122-123 0.2616858125349246 1105 124-125 0.3008925922963144 1106 1 0.09291066465948816 1106 2 0.12218872550960924 1106 3 0.10469643510445437 1106 4 -0.5746109699078801 1106 5 0.02350702763022383 1106 6 0.09109960233728032 1106 7 0.11767137016871487 1106 8 0.1561234560210849 1106 9 0.24538930304241546 1106 10-11 0.2739813606612671 1106 12-13 0.28900010323538794 1106 14-15 0.4329004287274003 1106 16-17 0.43053262216962906 1106 18-19 0.3020017115066196 1106 20-21 0.37604414351660154 1106 22-23 0.3765820747656008 1106 24-25 0.3926212488133558 1106 26-27 0.37429191538015516 1106 28-29 0.3471764016885146 1106 30-31 0.39347329382701446 1106 32-33 0.4587321135684306 1106 34-35 0.5091022649513377 1106 36-37 0.40703714894152654 1106 38-39 0.5209912598892501 1106 40-41 0.48376718369435423 1106 42-43 0.42507962491501416 1106 44-45 0.371218378556037 1106 46-47 0.4370743053537325 1106 48-49 0.3268068531816368 1106 50-51 0.3307013555951812 1106 52-53 0.39750885308995265 1106 54-55 0.42420679784941484 1106 56-57 0.3823376650834547 1106 58-59 0.4506515162011624 1106 60-61 0.4003618621975349 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-2.081483976765398 2216 84-85 -1.9812891599785374 2216 86-87 -1.9600536052059567 2216 88-89 -1.9960887653736101 2216 90-91 -2.1029843244675916 2216 92-93 -1.8618297366198462 2216 94-95 -1.8162747058786088 2216 96-97 -1.9333436258639694 2216 98-99 -1.860200486842686 2216 100-101 -1.8961337760741053 2216 102-103 -1.9230034903634667 2216 104-105 -2.266124738289747 2216 106-107 -2.1246408084424893 2216 108-109 -2.402793960488907 2216 110-111 -2.5798445640503544 2216 112-113 -2.7769490098348157 2216 114-115 -2.7290142409524556 2216 116-117 -2.7776699120826933 2216 118-119 -3.0800578862697527 2216 120-121 -2.7295308536385363 2216 122-123 -2.5041781812718646 2216 124-125 -2.394843647994378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19174.0 3 4651.0 4 3523.0 5 2576.0 6 2925.0 7 3377.0 8 3636.0 9 4132.0 10 4702.0 11 5030.0 12 4980.0 13 4901.0 14 4659.0 15 4467.0 16 4717.0 17 4607.0 18 4471.0 19 4551.0 20 4589.0 21 5214.0 22 6165.0 23 8049.0 24 11015.0 25 14888.0 26 17713.0 27 17426.0 28 20189.0 29 25037.0 30 30885.0 31 39971.0 32 53305.0 33 71132.0 34 114411.0 35 169205.0 36 351665.0 37 431927.0 38 34791.0 39 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.53118009538683 9.396169227197907 5.985153842907188 12.087496834508082 2 11.246968079466448 33.43143924600919 42.69023140394632 12.63136127057804 3 40.78914616173815 18.792119996470383 17.181863559198142 23.236870282593323 4 14.89979630638207 14.992917152082674 48.751462833652845 21.35582370788241 5 17.314981141219604 32.90119684773906 27.03252086055038 22.75130115049096 6 31.210188147014627 39.35745298958455 13.976767063539619 15.455591799861198 7 46.38092265632099 21.81902703756234 15.716052260417118 16.083998045699552 8 43.890008632377274 22.331123769589933 15.051613187849352 18.727254410183438 9 58.7727973682859 9.627011416297822 9.312599015214268 22.287592200202013 10-11 41.8548422143588 24.8453288523256 17.531635484418974 15.768193448896625 12-13 29.632990190333086 29.437099741490453 22.974558604496195 17.955351463680262 14-15 25.402151568506348 29.99214462838759 21.069219453929858 23.536484349176206 16-17 23.450358397214472 34.81302503578704 19.975920290036385 21.7606962769621 18-19 23.44403722642829 25.942084906492763 24.910845153703217 25.703032713375727 20-21 23.9216806939592 27.776673036124173 21.650306247555484 26.651340022361143 22-23 25.767297949701916 23.278995407932808 21.513512161010755 29.44019448135452 24-25 22.11698643450411 23.023383064266554 33.492427105707094 21.367203395522242 26-27 18.196528792676073 22.23371601972353 32.30711134274296 27.262643844857436 28-29 22.250970075416472 23.620352733744138 23.01764656493702 31.11103062590237 30-31 22.38698602626183 27.72221878362237 21.119492515338717 28.771302674777075 32-33 30.500274575856025 24.373446868584175 18.600440111515987 26.52583844404381 34-35 29.23692933326134 17.700726802949355 25.54796649252719 27.514377371262118 36-37 25.36702297877272 25.012938646452966 28.27858058109996 21.34145779367435 38-39 22.80678419654627 29.34409293174584 24.176173663632067 23.67294920807582 40-41 20.62798856394852 29.283482122280414 25.849670706509357 24.238858607261708 42-43 21.635359312467326 21.409805452799947 23.08132712980656 33.87350810492617 44-45 23.645359931415296 23.310667107392742 22.104508706754302 30.93946425443766 46-47 25.788269750695658 20.911519411920413 23.276690814417012 30.02352002296692 48-49 17.730225599951538 21.336980297700805 26.554843399578328 34.37795070276933 50-51 23.392414331674466 23.802368595370005 24.967143081017657 27.838073991937872 52-53 25.6670481317648 20.667199576481558 28.392295809722224 25.273456482031413 54-55 21.562204929723066 23.292230359266377 28.233311779896837 26.912252931113724 56-57 20.674445915367663 25.458641066676503 25.97944885044662 27.88746416750922 58-59 22.977719189889285 22.17351482117013 31.30484109498481 23.543924893955776 60-61 25.62657000536641 27.840199380393166 27.694186823642514 18.839043790597913 62-63 27.004897590448714 24.787812782724238 25.27279802674119 22.934491600085863 64-65 31.70817307913907 18.568547311779103 27.901086534960275 21.822193074121557 66-67 31.46626992488332 23.218217638196776 23.41180374845921 21.90370868846069 68-69 24.06055463749234 28.3309479054824 24.685869278553767 22.922628178471488 70-71 25.129183735296827 28.02799694411149 22.074348977199385 24.7684703433923 72-73 23.137614986983973 27.96133151643391 22.645485016999686 26.255568479582436 74-75 21.156477077003974 28.599260545328885 25.577532273353913 24.666730104313224 76-77 22.795958909566675 27.764389125165685 22.72882908331407 26.71082288195357 78-79 23.1560652497204 29.609824529520047 19.77469375714638 27.459416463613177 80-81 21.568139414752764 22.019580671913946 25.570235956413796 30.84204395691949 82-83 24.422624677809278 17.254969613904482 28.631409204358054 29.690996503928186 84-85 26.364572164690447 18.894248373933408 32.29523679059634 22.4459426707798 86-87 28.818701027784794 21.60833817040018 24.965520465649032 24.607440336165993 88-89 23.450464420242774 24.457347659392443 27.40818698121738 24.6840009391474 90-91 19.85689118587586 27.19159652190403 26.620221379471783 26.331290912748333 92-93 23.564842071711467 21.75743542530198 27.46385515985127 27.213867343135277 94-95 22.334246311919937 21.02684736574177 28.81055507737049 27.82835124496781 96-97 21.633085791330487 19.260040925920134 29.302629564450093 29.804243718299283 98-99 20.771383221343267 22.399757944742788 30.575411307918188 26.253447525995753 100-101 19.855994784996327 27.046411557290963 26.875541171861368 26.22205248585134 102-103 21.134208991075297 25.28860095370664 26.797484174027097 26.779705881190964 104-105 21.53383308141807 21.76752001796275 26.550398252187644 30.148248648431537 106-107 20.420427440051228 24.42660380557618 23.783122653203332 31.369846101169262 108-109 23.023975674027756 24.733523144044998 25.965163764415234 26.277337417512015 110-111 24.494042954989315 25.702966867846705 23.497437950465724 26.305552226698254 112-113 20.793629524324693 26.426036590003182 25.84287086235636 26.937463023315765 114-115 21.76127606712351 22.419874575555767 27.967438505179267 27.851410852141456 116-117 24.77016618094615 22.51291560051781 25.27358817308946 27.443330045446583 118-119 21.996201007243787 23.66884512926625 26.48626118816546 27.848692675324504 120-121 19.74095968051255 23.73534296997562 28.218768570784214 28.30492877872761 122-123 21.18783272515836 25.268822337729556 29.47051407714071 24.072830859971376 124-125 22.448116715235304 27.155429207788277 24.088426273866375 26.308027803110047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 5.5 2 10.0 3 12.0 4 13.0 5 13.5 6 13.5 7 18.0 8 23.5 9 27.5 10 41.0 11 58.0 12 70.5 13 84.0 14 97.0 15 121.5 16 175.0 17 219.5 18 264.5 19 338.0 20 419.0 21 522.0 22 628.5 23 725.5 24 887.5 25 1101.0 26 1380.5 27 1663.0 28 1850.5 29 2115.5 30 2582.5 31 3127.0 32 3622.5 33 4160.5 34 4931.0 35 5518.5 36 6235.0 37 7214.0 38 7865.0 39 8347.0 40 8780.0 41 9628.5 42 10880.0 43 12288.5 44 14217.0 45 21521.5 46 49294.5 47 99913.5 48 142122.5 49 173570.5 50 177669.5 51 140915.5 52 101137.0 53 74483.0 54 60502.0 55 53971.0 56 48079.5 57 41142.5 58 35239.0 59 29486.5 60 26179.0 61 22785.5 62 17225.0 63 12666.5 64 9759.0 65 8537.5 66 7654.0 67 6732.0 68 6304.0 69 6272.5 70 5683.5 71 4200.0 72 2801.5 73 2072.5 74 1731.5 75 1416.0 76 1024.5 77 866.5 78 754.0 79 507.0 80 507.0 81 587.0 82 548.5 83 294.5 84 67.0 85 45.0 86 41.0 87 26.0 88 16.0 89 11.5 90 9.0 91 8.0 92 8.0 93 6.0 94 3.0 95 2.5 96 1.0 97 1.0 98 1.5 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15500037531951544 2 0.018370902597342736 3 0.00842822771490993 4 0.016395536726660723 5 0.003292276451136691 6 0.0 7 2.633821160909353E-4 8 3.292276451136691E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.230691127841728E-4 28-29 0.009119605769648636 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.938414676705036E-4 58-59 0.0 60-61 6.584552902273383E-5 62-63 0.0 64-65 0.0016461382255683456 66-67 1.3169105804546765E-4 68-69 0.0072759309570120875 70-71 0.02146564246141123 72-73 9.876829353410073E-5 74-75 0.010173134234012376 76-77 4.6091870315913676E-4 78-79 0.001152296757897842 80-81 9.547601708296405E-4 82-83 9.876829353410073E-5 84-85 0.02093887822922936 86-87 0.003391044744670792 88-89 0.020247500174490653 90-91 9.876829353410073E-5 92-93 0.015276162733274248 94-95 0.01534200826229698 96-97 0.00714423989896662 98-99 0.0029301260415116556 100-101 7.243008192500721E-4 102-103 0.0 104-105 4.938414676705036E-4 106-107 8.889146418069066E-4 108-109 0.0 110-111 0.0 112-113 8.230691127841728E-4 114-115 0.006749166724830218 116-117 0.0 118-119 0.07891586653374649 120-121 0.0015473699320342452 122-123 0.0060248659055801455 124-125 0.2528139086827865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1518706.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.58104109054647 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42690288042114 25.55230145351298 2 10.75762764734288 7.009894168297542 3 3.5995850248853443 3.5183468281411536 4 1.7625148015676508 2.2969826869028798 5 1.009213939241501 1.6440620411789806 6 0.6949552814991267 1.35854199495692 7 0.500105791441909 1.1405777138412372 8 0.3721481760652159 0.9699980012922176 9 0.29829343355077026 0.8746839554002054 >10 2.0643151699289013 13.50168331754324 >50 0.2693119432248356 6.091022981750837 >100 0.20802942473995353 13.831683085705857 >500 0.022036158381944684 4.953864905887541 >1k 0.012938661802242754 8.733303728833217 >5k 8.086663626401721E-4 1.8166388876913453 >10k+ 0.0012129995439602581 6.706414249063888 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 20290 1.3360057838712693 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 20247 1.3331744261232918 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 17966 1.1829807744224359 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 16330 1.0752574889412434 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14859 0.978398715748802 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 12124 0.798311193871625 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 9572 0.6302734038056081 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 6799 0.4476837518255673 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 5971 0.3931636537947437 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 5238 0.3448988810210798 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 4827 0.3178363685927362 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4763 0.3136222547352812 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 4290 0.2824773195075281 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 4279 0.28175301868827807 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4067 0.2677937665354585 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 4016 0.26443564455529905 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3992 0.26285535185875347 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3877 0.255283116021139 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 3601 0.2371097500108645 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 3404 0.22413818079338593 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 3198 0.2105740018147028 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 3114 0.20504297737679317 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2985 0.19654890413286047 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2899 0.19088618863690537 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2645 0.17416142426513098 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 2565 0.16889378194331225 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2475 0.1629676843312662 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2388 0.1572391233062884 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2253 0.1483499768882193 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 2252 0.14828413135919657 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2248 0.14802074924310565 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2203 0.1450577004370826 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 2197 0.1446626272629462 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2152 0.1416995784569232 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 2142 0.14104112316669584 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 2031 0.13373226944517239 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1983 0.13057168405208117 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1875 0.12346036691762592 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1867 0.12293360268544405 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1817 0.11964132623430736 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1716 0.11299092780301125 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1616 0.10640637490073786 No Hit GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG 1615 0.10634052937171515 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1569 0.10331163503666937 No Hit TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA 1559 0.10265317974644204 No Hit GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG 1546 0.1017971878691465 No Hit CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA 1525 0.10041443175966909 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.584552902273383E-5 2 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 3 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 4 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 5 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 6 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 7 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 8 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 9 0.0 0.0 0.0 1.3169105804546765E-4 6.584552902273383E-5 10-11 3.292276451136691E-5 0.0 0.0 1.6461382255683458E-4 6.584552902273383E-5 12-13 6.584552902273383E-5 0.0 0.0 1.9753658706820148E-4 1.3169105804546765E-4 14-15 6.584552902273383E-5 0.0 0.0 1.9753658706820148E-4 1.3169105804546765E-4 16-17 6.584552902273383E-5 0.0 0.0 1.9753658706820148E-4 1.3169105804546765E-4 18-19 6.584552902273383E-5 0.0 0.0 2.633821160909353E-4 1.3169105804546765E-4 20-21 6.584552902273383E-5 0.0 0.0 9.547601708296405E-4 1.3169105804546765E-4 22-23 6.584552902273383E-5 0.0 0.0 0.001876597577147914 1.6461382255683458E-4 24-25 6.584552902273383E-5 0.0 0.0 0.0030288943350457558 1.9753658706820148E-4 26-27 1.3169105804546765E-4 0.0 0.0 0.005037182970239138 1.9753658706820148E-4 28-29 1.3169105804546765E-4 0.0 0.0 0.013037414746501299 1.9753658706820148E-4 30-31 1.3169105804546765E-4 0.0 0.0 0.047211244309300154 1.9753658706820148E-4 32-33 1.3169105804546765E-4 0.0 0.0 0.11671120019279571 1.9753658706820148E-4 34-35 1.3169105804546765E-4 0.0 0.0 0.19470522932022394 1.9753658706820148E-4 36-37 1.3169105804546765E-4 0.0 0.0 0.2876461935358127 1.9753658706820148E-4 38-39 1.3169105804546765E-4 0.0 0.0 0.4370826216529071 1.9753658706820148E-4 40-41 1.3169105804546765E-4 0.0 0.0 0.6754105139506923 1.9753658706820148E-4 42-43 1.3169105804546765E-4 0.0 0.0 1.2316735431347476 1.9753658706820148E-4 44-45 1.3169105804546765E-4 0.0 0.0 2.0325856354027705 1.9753658706820148E-4 46-47 1.3169105804546765E-4 0.0 0.0 2.8490043497556474 1.9753658706820148E-4 48-49 1.3169105804546765E-4 0.0 0.0 3.149194116570291 1.9753658706820148E-4 50-51 1.3169105804546765E-4 0.0 0.0 3.5401848679072843 1.9753658706820148E-4 52-53 1.3169105804546765E-4 0.0 0.0 3.8212465085408236 1.9753658706820148E-4 54-55 1.3169105804546765E-4 0.0 0.0 4.106489340267306 1.9753658706820148E-4 56-57 1.3169105804546765E-4 0.0 0.0 4.563984075917261 1.9753658706820148E-4 58-59 1.3169105804546765E-4 0.0 0.0 5.093118747143951 1.9753658706820148E-4 60-61 1.3169105804546765E-4 0.0 0.0 5.845766066638309 1.9753658706820148E-4 62-63 1.3169105804546765E-4 0.0 0.0 6.752459001281354 1.9753658706820148E-4 64-65 1.3169105804546765E-4 0.0 0.0 7.5840221873094595 1.9753658706820148E-4 66-67 1.3169105804546765E-4 0.0 0.0 8.261671449246924 1.9753658706820148E-4 68-69 1.3169105804546765E-4 0.0 0.0 8.801242636823718 1.9753658706820148E-4 70-71 1.3169105804546765E-4 0.0 0.0 9.441820865921382 1.9753658706820148E-4 72-73 1.3169105804546765E-4 0.0 0.0 10.042990545898942 1.9753658706820148E-4 74-75 1.3169105804546765E-4 0.0 0.0 10.661708059361061 1.9753658706820148E-4 76-77 1.3169105804546765E-4 0.0 0.0 11.393515268919725 1.9753658706820148E-4 78-79 1.3169105804546765E-4 0.0 0.0 12.350909260910274 1.9753658706820148E-4 80-81 1.3169105804546765E-4 0.0 0.0 14.567829454812188 1.9753658706820148E-4 82-83 1.3169105804546765E-4 0.0 0.0 16.147760000948175 1.9753658706820148E-4 84-85 1.3169105804546765E-4 0.0 0.0 16.861130462380473 1.9753658706820148E-4 86-87 1.3169105804546765E-4 0.0 0.0 17.437212995800373 1.9753658706820148E-4 88-89 1.3169105804546765E-4 0.0 0.0 18.0999811681787 1.9753658706820148E-4 90-91 1.3169105804546765E-4 0.0 0.0 18.937898447757497 2.3045935157956838E-4 92-93 1.3169105804546765E-4 0.0 0.0 20.037090786498506 2.633821160909353E-4 94-95 1.3169105804546765E-4 0.0 0.0 21.50077105114486 2.633821160909353E-4 96-97 1.3169105804546765E-4 0.0 0.0 23.210417289455627 2.633821160909353E-4 98-99 1.3169105804546765E-4 0.0 0.0 24.911602377286982 3.2922764511366916E-4 100-101 1.3169105804546765E-4 0.0 0.0 26.359051719029225 3.2922764511366916E-4 102-103 1.3169105804546765E-4 0.0 0.0 27.619466835582397 3.2922764511366916E-4 104-105 1.3169105804546765E-4 0.0 0.0 28.960378111365863 3.2922764511366916E-4 106-107 1.3169105804546765E-4 0.0 0.0 30.227015630411678 3.2922764511366916E-4 108-109 1.3169105804546765E-4 0.0 0.0 31.239193102549144 3.2922764511366916E-4 110-111 1.3169105804546765E-4 0.0 0.0 32.36185937238675 3.2922764511366916E-4 112-113 1.3169105804546765E-4 0.0 0.0 35.51813188332699 3.2922764511366916E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 3545 0.0 95.132324 1 AGTAGTA 2975 0.0 94.99277 2 AGTCGTA 615 0.0 93.83839 2 AGTTTCG 140 0.0 93.49288 2 GAGTAAG 1495 0.0 93.26154 1 GAGTTAG 1760 0.0 89.0371 1 GAGTCGT 985 0.0 88.92196 1 GAGTCAG 7995 0.0 86.82313 1 GAGTACG 855 0.0 85.716995 1 GAGTCGG 1175 0.0 84.17792 1 AGTAAGG 795 0.0 83.06915 2 GAGTCCG 855 0.0 82.92945 1 GAGTCGA 500 0.0 82.22559 1 AGTTAGG 475 0.0 81.414856 2 GAGTTCG 560 0.0 80.86368 1 GAGTAGG 1515 0.0 80.6249 1 GAGTGGT 1275 0.0 80.37967 1 AGTCGAG 230 0.0 80.18955 2 AGTCCGT 420 0.0 79.32729 2 AGTACGT 150 0.0 79.327286 2 >>END_MODULE