##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 860290 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.523867533041184 33.0 31.0 34.0 31.0 34.0 2 32.583196364016786 34.0 31.0 34.0 31.0 34.0 3 32.77856188029618 34.0 31.0 34.0 31.0 34.0 4 36.02574480698369 37.0 35.0 37.0 35.0 37.0 5 36.07684036778296 37.0 35.0 37.0 35.0 37.0 6 36.192979111694896 37.0 37.0 37.0 35.0 37.0 7 36.224954375850004 37.0 37.0 37.0 35.0 37.0 8 36.213723279359286 37.0 37.0 37.0 35.0 37.0 9 38.048589429145984 39.0 39.0 39.0 35.0 39.0 10-11 37.73788664287624 39.0 38.0 39.0 35.0 39.0 12-13 37.6402893210429 39.0 37.0 39.0 35.0 39.0 14-15 39.195303327947556 41.0 39.0 41.0 35.5 41.0 16-17 39.28334050145881 41.0 39.0 41.0 35.0 41.0 18-19 39.25857617780051 41.0 39.0 41.0 35.0 41.0 20-21 39.25866800729986 41.0 39.0 41.0 35.0 41.0 22-23 39.13646154203815 41.0 39.0 41.0 35.0 41.0 24-25 38.95113624475468 41.0 38.0 41.0 35.0 41.0 26-27 38.845023771053945 41.0 38.0 41.0 35.0 41.0 28-29 38.7226057492241 40.0 38.0 41.0 35.0 41.0 30-31 38.604911715816755 40.0 38.0 41.0 35.0 41.0 32-33 38.46358204791407 40.0 38.0 41.0 34.5 41.0 34-35 38.08420358251287 40.0 37.0 41.0 34.0 41.0 36-37 37.95712957258599 40.0 37.0 41.0 34.0 41.0 38-39 37.65814085947761 40.0 37.0 41.0 33.0 41.0 40-41 37.418235130014295 40.0 37.0 41.0 33.0 41.0 42-43 37.232323402573556 40.0 36.5 41.0 33.0 41.0 44-45 37.047906519894454 40.0 36.0 41.0 32.5 41.0 46-47 36.868890141696404 40.0 35.0 41.0 32.0 41.0 48-49 36.66613176951958 40.0 35.0 41.0 31.5 41.0 50-51 36.396127468644295 39.5 35.0 41.0 31.0 41.0 52-53 36.10839658719734 39.0 35.0 41.0 31.0 41.0 54-55 35.85032721524137 39.0 35.0 41.0 30.5 41.0 56-57 35.616853037929076 38.0 35.0 40.5 30.0 41.0 58-59 35.30223936114566 37.0 35.0 40.0 30.0 41.0 60-61 34.978391588882815 37.0 35.0 39.0 30.0 41.0 62-63 34.547268362993876 36.0 35.0 39.0 29.0 41.0 64-65 34.091360471468924 35.5 34.5 38.5 29.0 41.0 66-67 33.63649176440502 35.0 34.0 37.0 28.0 39.5 68-69 33.29085250322566 35.0 34.0 36.5 28.0 39.0 70-71 33.12484801636657 35.0 34.0 36.0 28.5 39.0 72-73 32.85270955143034 35.0 34.0 35.5 27.5 37.0 74-75 32.55564402701415 35.0 34.0 35.0 27.0 37.0 76-77 32.41798114589267 35.0 34.0 35.0 27.0 36.5 78-79 32.44924385962873 35.0 34.0 35.0 27.5 36.0 80-81 32.39197479919562 35.0 34.0 35.0 29.0 36.0 82-83 32.29863185669949 35.0 34.0 35.0 28.0 35.0 84-85 32.1286478978019 35.0 34.0 35.0 27.0 35.0 86-87 32.04421474154064 35.0 33.5 35.0 27.0 35.0 88-89 31.98508119355101 35.0 34.0 35.0 27.0 35.0 90-91 31.98861314208 35.0 34.0 35.0 27.0 35.0 92-93 32.00186216275907 35.0 34.0 35.0 27.0 36.0 94-95 31.914120819723582 35.0 34.0 35.0 27.0 35.0 96-97 31.87248602215532 35.0 34.0 35.0 27.0 35.0 98-99 31.777681363261227 35.0 33.5 35.0 25.0 35.0 100-101 31.716461890757767 35.0 33.0 35.0 25.0 35.0 102-103 31.017867812016878 34.5 32.0 35.0 24.0 35.0 104-105 31.598505736437712 35.0 33.0 35.0 25.0 35.0 106-107 31.703604017249997 35.0 33.0 35.0 25.0 35.0 108-109 31.635686803287264 35.0 33.0 35.0 25.0 35.0 110-111 31.550491694661105 35.0 33.0 35.0 25.0 35.0 112-113 31.49433214381197 35.0 33.0 35.0 25.0 35.0 114-115 31.36334724337142 35.0 33.0 35.0 24.0 35.0 116-117 31.196988224900906 35.0 33.0 35.0 23.5 35.0 118-119 30.965084448267447 35.0 33.0 35.0 19.5 35.0 120-121 30.631346406444337 35.0 33.0 35.0 8.5 35.0 122-123 30.24503771983866 35.0 33.0 35.0 2.0 35.0 124-125 27.920688953724905 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06680631122453207 1101 2 0.06327026006037784 1101 3 0.1478479196432545 1101 4 0.16633339526145363 1101 5 0.23514451970099515 1101 6 0.1897154453968568 1101 7 0.16259721485094758 1101 8 0.11812980334499201 1101 9 0.1511396074321354 1101 10-11 0.1235391840698199 1101 12-13 0.21691705353335777 1101 14-15 0.21944385072512773 1101 16-17 0.21009269073542214 1101 18-19 0.2090039561468373 1101 20-21 0.23159726044551832 1101 22-23 0.3460267205752814 1101 24-25 0.38746780432894923 1101 26-27 0.30023486107695874 1101 28-29 0.29505712358025704 1101 30-31 0.31530029157129746 1101 32-33 0.34340002221343013 1101 34-35 0.595330423927706 1101 36-37 0.3983962431218657 1101 38-39 0.5443365392959194 1101 40-41 0.5085686101126967 1101 42-43 0.5311848785990279 1101 44-45 0.6520094179990252 1101 46-47 0.6621118850070076 1101 48-49 0.7441923100347552 1101 50-51 0.8068465457124461 1101 52-53 0.8614123078952005 1101 54-55 0.810938738064479 1101 56-57 0.6400769417095304 1101 58-59 0.6153744591223287 1101 60-61 0.5515184806863331 1101 62-63 0.5599906738708 1101 64-65 0.48069060440401756 1101 66-67 0.21601649862665084 1101 68-69 0.3030595971725063 1101 70-71 0.4578823104262 1101 72-73 0.3942722412484798 1101 74-75 0.4076709858577061 1101 76-77 0.44491110127286504 1101 78-79 0.4271473680865725 1101 80-81 0.5149946116614146 1101 82-83 0.5150490097932092 1101 84-85 0.5013918998932709 1101 86-87 0.3926655073096015 1101 88-89 0.4270576521062921 1101 90-91 0.4381988293868915 1101 92-93 0.48028223102354417 1101 94-95 0.37334602079726054 1101 96-97 0.47167689731413986 1101 98-99 0.449139390804671 1101 100-101 0.28725985083565675 1101 102-103 0.3244023165797252 1101 104-105 0.40518287159805055 1101 106-107 0.3124586285915285 1101 108-109 0.26580381387824303 1101 110-111 0.3243945503185941 1101 112-113 0.4981211748622911 1101 114-115 0.5038562259993675 1101 116-117 0.41423371734790493 1101 118-119 0.0777144738541864 1101 120-121 0.2758859760432557 1101 122-123 0.2433726638186151 1101 124-125 0.020980567099567082 1102 1 -1.579744897526325E-4 1102 2 4.498567794470887E-4 1102 3 0.06273812811899404 1102 4 0.13304349437286334 1102 5 0.20679950944808922 1102 6 0.17020288558824603 1102 7 0.20395059830958928 1102 8 0.1609143214584563 1102 9 0.12663943655038423 1102 10-11 0.11689081599053708 1102 12-13 0.1519125678874218 1102 14-15 0.2202822393102224 1102 16-17 0.2706489108311203 1102 18-19 0.2278757093935937 1102 20-21 0.23464109161434976 1102 22-23 0.17620764162791147 1102 24-25 0.18829978484843224 1102 26-27 0.19069431938181225 1102 28-29 0.2196769116868893 1102 30-31 0.18007942690963574 1102 32-33 0.26281859705963484 1102 34-35 0.28068906200015675 1102 36-37 0.15614466246567815 1102 38-39 0.14764523717698808 1102 40-41 0.21811235584065258 1102 42-43 0.239703333828011 1102 44-45 0.16810434281105557 1102 46-47 0.23833048548547708 1102 48-49 0.21601284147699573 1102 50-51 0.1397295344342453 1102 52-53 0.27450291794305315 1102 54-55 0.20105109281499267 1102 56-57 0.08470057465553538 1102 58-59 0.07774309377715127 1102 60-61 0.10568265532748455 1102 62-63 0.19442121043949356 1102 64-65 0.21654479955098083 1102 66-67 0.14543699589254544 1102 68-69 0.18383753633860778 1102 70-71 0.2724222113147903 1102 72-73 0.2634889620277008 1102 74-75 0.17135754600808184 1102 76-77 0.15756275882583282 1102 78-79 0.1539021100551281 1102 80-81 0.15861538233810535 1102 82-83 0.26449364410625975 1102 84-85 0.1776030242951876 1102 86-87 0.19852247928498912 1102 88-89 0.23477296311107665 1102 90-91 0.2068249401182669 1102 92-93 0.15975078878157944 1102 94-95 0.265893440482845 1102 96-97 0.07530599847613573 1102 98-99 0.07664921650528811 1102 100-101 0.20747409382950366 1102 102-103 0.07290710126871858 1102 104-105 0.008902753689639553 1102 106-107 0.15509789721764022 1102 108-109 0.20996286206689518 1102 110-111 0.10081180083124153 1102 112-113 -0.08972806471391692 1102 114-115 -0.25764625178600653 1102 116-117 -0.11373214902256734 1102 118-119 -0.11813737166870908 1102 120-121 -0.1885011992814185 1102 122-123 -0.2827989441786478 1102 124-125 -0.28404335634541056 1103 1 0.05331313305438812 1103 2 0.0965847612008659 1103 3 0.07573426119939342 1103 4 0.14012980693670585 1103 5 0.12902335302024426 1103 6 0.2014939260084958 1103 7 0.20164321468198665 1103 8 0.17277044286514354 1103 9 0.23543303059678777 1103 10-11 0.24739935239794164 1103 12-13 0.08531764595542057 1103 14-15 0.21372489216298618 1103 16-17 0.24917565094493455 1103 18-19 0.2751405933860056 1103 20-21 0.2893570919061901 1103 22-23 0.3757461649023881 1103 24-25 0.2878866446178705 1103 26-27 0.2515983030386053 1103 28-29 0.3783710967153411 1103 30-31 0.2335505344537836 1103 32-33 0.3462446513424311 1103 34-35 0.5098998091182096 1103 36-37 0.5318423496099953 1103 38-39 0.5495175923499076 1103 40-41 0.5460253136693396 1103 42-43 0.40341813026751794 1103 44-45 0.35550402178377283 1103 46-47 0.5147422759422113 1103 48-49 0.5916284477807991 1103 50-51 0.5425184589928378 1103 52-53 0.54704778880253 1103 54-55 0.5533922207881403 1103 56-57 0.553464254795621 1103 58-59 0.7150004992929837 1103 60-61 0.6085463646518647 1103 62-63 0.7273037071562598 1103 64-65 0.6727637912334572 1103 66-67 0.7158851572817184 1103 68-69 0.6324914105542803 1103 70-71 0.6217765087027942 1103 72-73 0.5650311698199104 1103 74-75 0.609258830117227 1103 76-77 0.6104209960941844 1103 78-79 0.5980871385098183 1103 80-81 0.6897149007971777 1103 82-83 0.6978576817540088 1103 84-85 0.7927122830139979 1103 86-87 0.7711001824806729 1103 88-89 0.617324400437802 1103 90-91 0.6406677826689773 1103 92-93 0.5823667963694987 1103 94-95 0.6220194072127434 1103 96-97 0.6125843802067692 1103 98-99 0.6591495813090198 1103 100-101 0.6507790332720909 1103 102-103 0.6298164840207967 1103 104-105 0.6492381907245175 1103 106-107 0.6305238055789388 1103 108-109 0.5601516480000654 1103 110-111 0.7139602394712554 1103 112-113 0.6911936119240565 1103 114-115 0.8031574108434931 1103 116-117 0.8041505300960665 1103 118-119 0.8658523043855695 1103 120-121 0.8409567023674924 1103 122-123 0.580837419457712 1103 124-125 0.6893350910498945 1104 1 0.058461283157171806 1104 2 0.023061917934008136 1104 3 0.08948218398293761 1104 4 0.21056916637527934 1104 5 0.22990431162266134 1104 6 0.22844721743923202 1104 7 0.06658520708494109 1104 8 0.05939014395192288 1104 9 0.20312885531125602 1104 10-11 0.31958897989559176 1104 12-13 0.2784070637684479 1104 14-15 0.3112401486823302 1104 16-17 0.2984679492506004 1104 18-19 0.362133968175705 1104 20-21 0.31378200382963684 1104 22-23 0.3717268878187667 1104 24-25 0.30052789855508877 1104 26-27 0.334112549107175 1104 28-29 0.33899151348444434 1104 30-31 0.3511686123833542 1104 32-33 0.3768770776728445 1104 34-35 0.40879359079133337 1104 36-37 0.25640183812883066 1104 38-39 0.3300254822326565 1104 40-41 0.4033107280378161 1104 42-43 0.1262498766997311 1104 44-45 0.18076329572662075 1104 46-47 0.15276148351712493 1104 48-49 0.10063626236244971 1104 50-51 0.1800906869144825 1104 52-53 0.2582274932875137 1104 54-55 0.2150421388579602 1104 56-57 0.1323115943495523 1104 58-59 0.27632488488099227 1104 60-61 0.07838987993928015 1104 62-63 0.21945694555441975 1104 64-65 0.23629121692670196 1104 66-67 0.23804073838100948 1104 68-69 0.18425802603336194 1104 70-71 0.17128988333803363 1104 72-73 0.12723760812898632 1104 74-75 0.15642886001884904 1104 76-77 0.13885617471227363 1104 78-79 0.20429838579222093 1104 80-81 0.18042486796074542 1104 82-83 0.3061120347372821 1104 84-85 0.3737533304845826 1104 86-87 0.22111826761855014 1104 88-89 0.32733146715715833 1104 90-91 0.3610821704581042 1104 92-93 0.26633190814337127 1104 94-95 0.33328342319460447 1104 96-97 0.3463412232753953 1104 98-99 0.4354492977392077 1104 100-101 0.31887685005483846 1104 102-103 0.394715805795947 1104 104-105 0.34389927781507623 1104 106-107 0.35398724463846065 1104 108-109 0.3703207730011897 1104 110-111 0.35566598333678456 1104 112-113 0.2170897916478438 1104 114-115 0.26371475488975804 1104 116-117 0.322986104656394 1104 118-119 0.2998238521515759 1104 120-121 0.178583700582152 1104 122-123 0.3809004278206416 1104 124-125 0.42101697025661977 1105 1 0.06147094537087128 1105 2 0.14073335377025842 1105 3 0.13860128934800997 1105 4 0.1098707829379606 1105 5 0.12346287656877308 1105 6 0.11712638891418692 1105 7 0.1623565216545373 1105 8 0.17625721027695818 1105 9 0.15868132758617293 1105 10-11 0.1421026468426092 1105 12-13 0.15250153684529266 1105 14-15 0.13994489372898045 1105 16-17 0.17779968688433456 1105 18-19 0.11449176102488678 1105 20-21 0.09783201654308016 1105 22-23 0.17353995870108463 1105 24-25 0.27332908481675133 1105 26-27 0.1781131505506437 1105 28-29 0.12187836876638869 1105 30-31 0.16358334677026676 1105 32-33 0.34213638293229565 1105 34-35 0.36299128721397267 1105 36-37 0.42032941064432805 1105 38-39 0.22509712979656626 1105 40-41 0.10227849457996285 1105 42-43 0.007075443425726746 1105 44-45 0.06967228706192685 1105 46-47 0.1563252194100926 1105 48-49 0.16431225547763262 1105 50-51 0.1251933846084654 1105 52-53 0.19073074499404186 1105 54-55 0.18571810263443922 1105 56-57 0.12152350781993704 1105 58-59 0.10419225437265567 1105 60-61 0.09487334268890635 1105 62-63 0.17295601405051997 1105 64-65 0.32629594779298543 1105 66-67 0.22232466614911317 1105 68-69 0.19803713247418386 1105 70-71 0.221661995920428 1105 72-73 0.2553621802728685 1105 74-75 0.2713684345098244 1105 76-77 0.3632960499249833 1105 78-79 0.3602045728362455 1105 80-81 0.5055774903520387 1105 82-83 0.386538900678957 1105 84-85 0.33413018615771506 1105 86-87 0.3274175933172998 1105 88-89 0.32654818484056847 1105 90-91 0.23864411180024803 1105 92-93 0.36719654424562265 1105 94-95 0.3982778243916165 1105 96-97 0.3055384184951464 1105 98-99 0.3325636526788678 1105 100-101 0.36822302477660784 1105 102-103 0.27901159129089237 1105 104-105 0.43124793899214353 1105 106-107 0.3606975567045012 1105 108-109 0.4252730853801694 1105 110-111 0.43055027881666774 1105 112-113 0.432662654323142 1105 114-115 0.4420983633549511 1105 116-117 0.455602860480127 1105 118-119 0.5314211490788345 1105 120-121 0.5264576223847186 1105 122-123 0.4987381163218245 1105 124-125 0.4316232179339714 1106 1 0.04294878434281202 1106 2 0.06183269437195804 1106 3 0.1095629430889673 1106 4 0.12778061209385783 1106 5 0.1326478546512746 1106 6 0.1302206903641121 1106 7 0.09997594393171028 1106 8 0.09936565507108241 1106 9 0.1026640839459958 1106 10-11 0.09697390494459057 1106 12-13 0.20192304128499927 1106 14-15 0.13514224489603777 1106 16-17 0.16444429555392048 1106 18-19 0.09305253525590018 1106 20-21 0.11780597780342816 1106 22-23 0.06148029907798502 1106 24-25 0.14561632583432527 1106 26-27 0.05584932566584655 1106 28-29 0.15759148367403952 1106 30-31 0.1759511627007342 1106 32-33 0.03220747431141291 1106 34-35 -0.0035022171507961275 1106 36-37 0.1347134726877215 1106 38-39 -0.051152940451835605 1106 40-41 -0.0707526769759923 1106 42-43 -0.10519437598905768 1106 44-45 -0.17329350665131216 1106 46-47 -0.12300173068544495 1106 48-49 -0.04467438796023515 1106 50-51 -0.10076585727081522 1106 52-53 -0.15256732364332493 1106 54-55 -0.11992799530118958 1106 56-57 -0.002551554935301681 1106 58-59 0.051332676237699104 1106 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-1.5219610667206531 2216 108-109 -1.2786569428779657 2216 110-111 -1.2083074142492904 2216 112-113 -1.108649025392964 2216 114-115 -1.1755220153603823 2216 116-117 -1.194031180245382 2216 118-119 -1.047070279599886 2216 120-121 -1.2771295647573595 2216 122-123 -1.2559876107853292 2216 124-125 -1.6812676197648209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 16.0 4 49.0 5 78.0 6 235.0 7 525.0 8 756.0 9 1203.0 10 2693.0 11 8426.0 12 12111.0 13 3843.0 14 3168.0 15 4300.0 16 2665.0 17 1840.0 18 1660.0 19 1563.0 20 1555.0 21 1599.0 22 1935.0 23 2219.0 24 2848.0 25 3721.0 26 4899.0 27 6371.0 28 8456.0 29 10830.0 30 13998.0 31 18283.0 32 24601.0 33 33940.0 34 59043.0 35 92406.0 36 182563.0 37 305950.0 38 39800.0 39 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.05287228317215 9.456273312473936 6.007949247826887 12.482905156527027 2 11.27690984330683 31.505207606825685 44.688241037801646 12.52964151206584 3 38.73984354113148 19.61722209952458 18.52654337490846 23.11639098443548 4 15.450623297149393 15.612281357182844 47.4107360038919 21.526359341775862 5 16.968938726248254 32.09424656005172 28.57581373932901 22.36100097437102 6 30.6927873304009 38.63151311690365 14.776161736397201 15.899537816298254 7 46.213137430401375 21.60352904253217 16.795731671878087 15.387601855188368 8 44.777458763905194 21.31816015529647 16.004021899591997 17.900359181206337 9 56.278464238803195 8.73042811145079 12.848225598344746 22.142882051401273 10-11 37.881877041462765 24.617628939078685 21.835892547861768 15.664601471596788 12-13 28.846609864115592 27.493926466656593 26.1629218054377 17.496541863790117 14-15 24.737181648048914 27.484162317358102 24.863185669948507 22.91547036464448 16-17 22.442374083158004 33.449185739692425 23.570075207197572 20.538364969951992 18-19 23.05577189087401 25.013309465412824 27.225412360948052 24.705506282765114 20-21 23.05710864940892 26.113926699136336 25.18476327749945 25.644201373955294 22-23 24.302909484011206 23.156086900928756 25.26909530507155 27.27190830998849 24-25 20.730974438852016 21.489207127828987 37.09772286089574 20.682095572423254 26-27 17.264527078078324 22.098478420067654 34.3336549303142 26.30333957153983 28-29 21.149728579897477 22.78842018389148 25.45060386613816 30.61124737007288 30-31 21.13851143219147 27.381987469341734 24.531320833672364 26.94818026479443 32-33 29.764033058619766 23.038510269792745 22.07418428669402 25.123272384893465 34-35 27.884492438596286 17.19234211719304 28.86578944309477 26.0573760011159 36-37 24.399330458333818 24.33958316381685 30.811296190819377 20.449790187029954 38-39 22.65863836613235 28.832777319276058 25.518371711864603 22.990212602726988 40-41 20.587243836380758 27.422380825070615 27.923839635471758 24.06653570307687 42-43 21.84240198072743 21.463808715665646 24.832440223645516 31.86134907996141 44-45 23.02124864871148 22.81097071917609 24.17237210708017 29.995408525032257 46-47 24.34783619477153 21.379244208348346 24.332725011333387 29.940194585546738 48-49 17.45452114984482 21.880412419067987 26.50606190935615 34.15900452173105 50-51 23.105406316474678 23.506782596566275 25.91870183310279 27.469109253856256 52-53 24.70864475932534 21.217089586069815 29.409210847504912 24.66505480709993 54-55 20.989201315835356 23.610236083181253 29.028873984354114 26.371688616629275 56-57 20.84767927094352 25.962408025200805 27.368852363737812 25.821060340117867 58-59 23.853003057108648 21.820258284996918 31.542561229352895 22.78417742854154 60-61 25.279673133478248 28.381766613583792 27.47759476455614 18.860965488381826 62-63 26.254693184856272 23.558567459810064 26.45235908821444 23.734380267119228 64-65 30.704587987771564 18.75698892234014 28.48405770147276 22.054365388415533 66-67 30.471585163142663 23.80755326692162 23.79802159736833 21.92283997256739 68-69 22.530077067035535 28.418324053516837 24.979541782422206 24.07205709702542 70-71 24.31156935451999 27.440514245196386 22.55198828302084 25.695928117262785 72-73 23.53159980936661 27.8757744481512 23.52561345592765 25.067012286554537 74-75 20.85506050285369 27.302769996164084 26.630089853421516 25.21207964756071 76-77 22.92907042973881 28.65946366922782 22.531355705634144 25.880110195399226 78-79 23.26558486091899 29.317962547513048 19.31929930604796 28.097153285519997 80-81 22.133931581210987 22.180369410315127 24.622046054237526 31.063652954236364 82-83 24.318892466493857 18.37636145950784 27.754536261028257 29.550209812970046 84-85 25.76851991770217 20.072591800439387 30.667623708284413 23.49126457357403 86-87 27.176126654965188 23.20049053226238 23.87009031838101 25.753292494391427 88-89 22.62266212556231 25.530053819061017 26.30973276453289 25.537551290843787 90-91 19.549221774052935 27.156423996559298 25.74062234827791 27.55373188110986 92-93 24.099838426577083 22.526589870857503 26.1101489032768 27.263422799288612 94-95 21.739587813411756 21.75388531774169 28.461972125678553 28.044554743168003 96-97 21.754757116786198 20.599623382812773 27.822943426054003 29.822676074347022 98-99 19.74188936288926 22.737448999755898 29.488951400109265 28.031710237245576 100-101 19.17376698555138 26.5430261888433 27.508979530158435 26.774227295446885 102-103 20.332678515384348 26.499145636936383 26.20418696021109 26.963988887468176 104-105 19.57514326564298 23.32771507282428 24.696265212893326 32.40087644863941 106-107 19.38497483406758 25.330876797359032 22.981029652791502 32.303118715781885 108-109 22.018621627590697 24.72201234467447 25.278917574306337 27.980448453428497 110-111 23.535141776597023 27.39249278868089 22.18883514020734 26.883530294514745 112-113 19.917004489778126 27.57421210932392 23.910843758627205 28.597939642270752 114-115 21.284981139971055 24.156180800548654 25.099937811306717 29.45890024817357 116-117 24.496321108334772 23.25289699277706 23.71026642975755 28.54051546913061 118-119 20.99451046034832 25.035381230947173 25.06269815668872 28.907410152015782 120-121 17.99839123642315 26.328334232257106 26.65682887591132 29.016445655408425 122-123 18.4002329503911 27.680469713543086 28.109114707143927 25.810182628921886 124-125 19.299685771342695 27.818741515680912 23.95875785208289 28.922814860893503 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 2.0 2 15.5 3 50.5 4 83.5 5 118.5 6 175.0 7 231.0 8 292.0 9 376.5 10 476.5 11 534.5 12 622.0 13 763.0 14 832.5 15 876.0 16 926.0 17 968.5 18 1060.5 19 1169.0 20 1225.0 21 1284.0 22 1394.5 23 1457.0 24 1539.0 25 1601.0 26 1737.5 27 1908.5 28 1955.0 29 2121.5 30 2380.0 31 2625.0 32 2956.0 33 3365.5 34 3938.0 35 4333.0 36 4734.0 37 5424.5 38 5821.5 39 6031.0 40 6226.0 41 6750.0 42 7417.0 43 8317.5 44 9267.0 45 11889.0 46 24137.5 47 50319.0 48 74650.0 49 89754.5 50 91930.0 51 78330.0 52 60011.0 53 43832.5 54 34448.0 55 30465.0 56 27241.5 57 23435.5 58 19830.0 59 15689.5 60 12926.5 61 11342.0 62 9200.0 63 6972.0 64 5463.5 65 4882.5 66 4294.5 67 3864.5 68 3729.0 69 3582.5 70 3110.5 71 2247.5 72 1582.0 73 1267.5 74 1010.0 75 847.5 76 588.5 77 415.0 78 383.5 79 288.0 80 280.0 81 302.5 82 231.5 83 98.0 84 14.0 85 9.0 86 7.5 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21434632507642773 2 0.0011623987260109964 3 0.0 4 0.12426042381057549 5 0.02882748840507271 6 0.0030222366876285905 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.001220518662311546 112-113 2.905996815027491E-4 114-115 5.811993630054982E-4 116-117 0.005172674330748934 118-119 0.0020341977705192436 120-121 2.3247974520219927E-4 122-123 0.002441037324623092 124-125 0.028769368468772157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 860290.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.623341019677554 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38484849131694 30.2434656760393 2 9.7883945155235 7.365442097853643 3 3.093938144011028 3.4921286965774545 4 1.5477575141627158 2.3292723508444904 5 0.9379624340761434 1.7644640260446784 6 0.632656850937838 1.428159867076175 7 0.4726074829313183 1.2446750749143503 8 0.3567065763338518 1.0736394532296119 9 0.28124193281966314 0.9523135032756689 >10 2.083276923088821 15.258825417561873 >50 0.22481899266919114 5.8326065672136025 >100 0.1707759441598102 12.942692129659822 >500 0.013587959390653362 3.41997524876815 >1k 0.009264517766354565 6.09844269941711 >5k 0.0018529035532709132 5.316269321645506 >10k+ 3.088172588784855E-4 1.2376278698786591 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10652 1.2381871229469132 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9845 1.1443815457578257 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8990 1.0449964546838857 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 8986 1.0445314951934812 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7631 0.8870264678189913 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5183 0.6024712596914994 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5121 0.5952643875902313 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3310 0.38475397830963975 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3146 0.36569063920305944 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3103 0.36069232468121215 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2852 0.33151611665833614 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2810 0.32663404200908996 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2364 0.2747910588289995 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2025 0.23538574201722676 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2014 0.23410710341861465 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1943 0.22585407246393657 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1856 0.21574120354764093 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1851 0.2151600041846354 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1838 0.2136488858408211 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1783 0.20725569284776066 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1691 0.19656162456845946 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1674 0.19458554673424078 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1644 0.19109835055620777 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1500 0.17435980890164945 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1421 0.16517685896616258 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1294 0.15041439514582292 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1209 0.14053400597472945 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1189 0.13820920852270746 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1188 0.13809296865010637 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1181 0.13727928954189866 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1148 0.13344337374606238 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1145 0.13309465412825908 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1098 0.1276313801160074 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1077 0.1251903427913843 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1070 0.1243766636831766 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1037 0.12054074788734032 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1027 0.11937834916132932 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 989 0.11496123400248753 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 967 0.11240395680526334 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 950 0.11042787897104465 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 945 0.10984667960803915 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 909 0.10566204419439956 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.7435980890164945E-4 0.0 20-21 0.0 0.0 0.0 8.136791082076975E-4 0.0 22-23 0.0 0.0 0.0 0.00319659649653024 0.0 24-25 0.0 0.0 0.0 0.004707714840344535 0.0 26-27 0.0 0.0 0.0 0.007729951527973126 0.0 28-29 0.0 0.0 0.0 0.017377860953864396 0.0 30-31 0.0 0.0 0.0 0.05608573853003057 0.0 32-33 0.0 0.0 0.0 0.12803821967011125 0.0 34-35 0.0 0.0 0.0 0.214695044694231 0.0 36-37 0.0 0.0 0.0 0.323786165130363 0.0 38-39 0.0 0.0 0.0 0.49675109556079927 0.0 40-41 0.0 0.0 0.0 0.7428890257936277 0.0 42-43 0.0 0.0 0.0 1.3013053737693103 0.0 44-45 0.0 0.0 0.0 2.1024886956723896 0.0 46-47 0.0 0.0 0.0 2.9086122121610156 0.0 48-49 0.0 0.0 0.0 3.224203466273001 0.0 50-51 0.0 0.0 0.0 3.622092550186565 0.0 52-53 0.0 0.0 0.0 3.924316218949424 0.0 54-55 0.0 0.0 0.0 4.228167245928699 0.0 56-57 0.0 0.0 0.0 4.701379767287776 0.0 58-59 0.0 0.0 0.0 5.254390961187506 0.0 60-61 0.0 0.0 0.0 6.027386113984819 0.0 62-63 0.0 0.0 0.0 6.953876018551884 0.0 64-65 0.0 0.0 0.0 7.794464657266736 0.0 66-67 0.0 0.0 0.0 8.454416533959478 0.0 68-69 0.0 0.0 0.0 9.008764486394123 0.0 70-71 0.0 0.0 0.0 9.65534877773774 0.0 72-73 0.0 0.0 0.0 10.252705483034791 0.0 74-75 0.0 0.0 0.0 10.900394053168117 0.0 76-77 0.0 0.0 0.0 11.687686710295367 0.0 78-79 0.0 0.0 0.0 12.673982029315695 0.0 80-81 0.0 0.0 0.0 15.05503957967662 0.0 82-83 0.0 0.0 0.0 16.754059677550593 0.0 84-85 0.0 0.0 0.0 17.489160631879948 0.0 86-87 0.0 0.0 0.0 18.10046612188913 0.0 88-89 0.0 0.0 0.0 18.798428436922435 0.0 90-91 0.0 0.0 0.0 19.687547222448245 0.0 92-93 0.0 0.0 0.0 20.859768217694032 0.0 94-95 0.0 0.0 0.0 22.413023515326227 0.0 96-97 0.0 0.0 0.0 24.207709028350905 0.0 98-99 0.0 0.0 0.0 25.888595706099103 0.0 100-101 0.0 0.0 0.0 27.340838554440943 0.0 102-103 0.0 0.0 0.0 28.597914656685536 0.0 104-105 0.0 0.0 0.0 29.922293645166164 0.0 106-107 0.0 0.0 0.0 31.18692533912983 0.0 108-109 0.0 0.0 0.0 32.22587732043846 0.0 110-111 0.0 0.0 0.0 33.305861976775276 0.0 112-113 0.0 0.0 0.0 36.58371014425368 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCCGT 225 0.0 95.35268 2 TATAACG 25 1.7117933E-5 95.35268 2 GTATACG 90 0.0 92.909546 1 AGTAGTA 1215 0.0 90.25151 2 GAGTACG 420 0.0 88.16926 1 GAGTCCG 600 0.0 86.604965 1 GAGTAGT 1650 0.0 86.15248 1 GAGTCAG 3830 0.0 79.06495 1 GAGTAAT 435 0.0 78.2637 1 AGTACGG 230 0.0 77.733154 2 GAGTAAG 785 0.0 77.60779 1 GAGTTAG 895 0.0 77.41226 1 GAGTAGG 890 0.0 75.83388 1 GAGTCGG 605 0.0 75.02967 1 GAGTAGC 665 0.0 73.649025 1 TCGTAGG 1065 0.0 72.74559 2 ATACGGG 115 0.0 72.54672 3 AGTCGGT 305 0.0 72.29609 2 GAGTCGT 615 0.0 71.86731 1 GAGTGGT 700 0.0 71.67308 1 >>END_MODULE