FastQCFastQC Report
Thu 26 May 2016
SRR935940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404820
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8340.20601748925448346No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT8240.20354725557037695No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7160.17686873178202658No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6170.15241341830937205No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC5770.142532483572946No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5300.13092238525764535No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAA300.00423946659.4826472
TAACAAC1850.054.727321
TGTATGG751.4713933E-647.586122
AGTAATA951.4877332E-743.8293232
GTGGAAA905.113914E-639.704141
GAAAACA2850.039.704141
GTGGCAC300.001310160639.65509820-21
TATGTGG758.193317E-539.6550982
GTGGAAT2000.038.7115361
GTAATAG1104.700778E-737.8525923
GTATAGG1751.0913936E-1137.4353331
CGAATAG401.19747194E-437.18583764-65
ATGGGAC801.19815435E-437.1766555
GACCTTA650.001940194436.6047069
GTGTAAC700.002773988334.032121
TCGAATA350.002789111333.99848664-65
CTTAAAT350.00279249233.99008612-13
TTATAGG700.002790850833.9900862
TTAAGAG350.00279249233.9900867
TTAAATG350.00279249233.99008612-13