FastQCFastQC Report
Thu 26 May 2016
SRR935940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404820
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT75291.859838940763796No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT39680.9801887258534657TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT23400.5780346820809248RNA PCR Primer, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT10180.25146978904204337TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG9670.23887159725310014TruSeq Adapter, Index 11 (96% over 28bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8090.19984190504421717No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7810.19292525072871894No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7150.17662170841361594No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC7030.1736574279926881No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6630.16377649325626203No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6270.1548836519934786No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG6030.14895509115162295TruSeq Adapter, Index 11 (95% over 24bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5230.12919322167877081No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT4790.11832419346870214No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4550.11239563262684649No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG152.6866214E-4119.1533662
GCGTTAG250.002027397771.651541
GTCAGTA250.002045404271.492022
TCACATC453.1483828E-452.957053
AAGGTTT350.00779483250.9963465
AGGGTCG602.1917744E-549.579786
GTATGGT255.333547E-447.5965914-15
ATGGTTT255.333547E-447.5965916-17
CTACGGG802.2699714E-644.6825143
ATGTGTA1256.220944E-1042.836936
TGTGTAT1309.131327E-1041.1893587
GTCAGAT758.117387E-539.7177933
TCATAGG758.1827E-539.6638269
GGGTGTA600.001307969439.6638227
GTACCGA600.001307969439.6638226
TGTGTTA300.001308744139.66382252-53
GTTATGT300.001308744139.66382214-15
TCTACGG957.394623E-637.627382
GTTTAGG801.1715896E-437.3185121
AGATGTG1452.6266207E-936.9786344
Produced by FastQC (version 0.11.5)