##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 404820 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28437083148066 33.0 31.0 34.0 30.0 34.0 2 32.390111654562524 34.0 31.0 34.0 31.0 34.0 3 32.37769873029988 34.0 31.0 34.0 30.0 34.0 4 35.88747097475421 37.0 35.0 37.0 35.0 37.0 5 35.87740477249148 37.0 35.0 37.0 35.0 37.0 6 35.961775603972136 37.0 35.0 37.0 35.0 37.0 7 35.93451657526802 37.0 35.0 37.0 35.0 37.0 8 35.96200533570476 37.0 35.0 37.0 35.0 37.0 9 37.600521219307346 39.0 37.0 39.0 35.0 39.0 10-11 37.54620201571069 39.0 37.0 39.0 35.0 39.0 12-13 37.541367768390884 39.0 37.0 39.0 35.0 39.0 14-15 38.65895459710488 40.0 38.0 41.0 34.0 41.0 16-17 38.57628699174942 40.0 38.0 41.0 34.0 41.0 18-19 38.65407712069562 40.0 38.0 41.0 34.0 41.0 20-21 38.76014648485747 40.0 38.0 41.0 34.0 41.0 22-23 38.59195197865718 40.0 38.0 41.0 34.0 41.0 24-25 38.297775554567465 40.0 38.0 41.0 33.0 41.0 26-27 38.117748629020305 40.0 37.0 41.0 33.0 41.0 28-29 38.014822637221485 40.0 37.0 41.0 33.0 41.0 30-31 37.83789214959735 40.0 37.0 41.0 33.0 41.0 32-33 37.49634528926437 40.0 36.0 41.0 33.0 41.0 34-35 37.34338718442765 40.0 35.0 41.0 32.0 41.0 36-37 37.25709451114076 40.0 35.0 41.0 32.5 41.0 38-39 36.83136702732078 39.5 35.0 41.0 31.5 41.0 40-41 36.603706585642996 39.0 35.0 41.0 31.0 41.0 42-43 36.32499505953263 39.0 35.0 41.0 31.0 41.0 44-45 35.90458598883454 39.0 35.0 41.0 30.0 41.0 46-47 35.39487550022232 38.0 35.0 41.0 28.5 41.0 48-49 34.723751296872685 37.5 35.0 40.0 27.0 41.0 50-51 33.991995207746655 37.0 34.0 39.5 25.0 41.0 52-53 33.50798626550072 36.0 34.0 39.0 23.0 41.0 54-55 32.985436737315354 35.5 34.0 38.5 22.0 41.0 56-57 32.60399313275036 35.0 33.0 37.0 22.0 40.0 58-59 32.27943406946297 35.0 33.0 37.0 21.0 39.5 60-61 31.808852082406993 35.0 33.0 36.0 19.0 39.0 62-63 31.383679166049106 35.0 33.0 36.0 16.5 38.5 64-65 31.0496504619337 35.0 33.0 35.0 9.0 37.0 66-67 30.58985228002569 35.0 32.5 35.0 2.0 36.5 68-69 30.090731683217232 35.0 32.0 35.0 2.0 36.0 70-71 29.568241440640286 35.0 31.5 35.0 2.0 35.5 72-73 29.25084111456944 35.0 31.0 35.0 2.0 35.0 74-75 29.1285978953609 35.0 31.0 35.0 2.0 35.0 76-77 29.00480583963243 35.0 31.0 35.0 2.0 35.0 78-79 29.086950990563707 35.0 31.0 35.0 2.0 35.0 80-81 29.065498246134084 35.0 31.0 35.0 2.0 35.0 82-83 28.961582925744775 35.0 31.0 35.0 2.0 35.0 84-85 28.903859740131416 35.0 31.0 35.0 2.0 35.0 86-87 28.88679042537424 35.0 31.0 35.0 2.0 35.0 88-89 28.82643150041994 35.0 31.0 35.0 2.0 35.0 90-91 28.715199347858306 35.0 30.0 35.0 2.0 35.0 92-93 28.62823724124302 35.0 30.0 35.0 2.0 35.0 94-95 28.601648880984143 35.0 30.0 35.0 2.0 35.0 96-97 28.56913937058446 35.0 30.0 35.0 2.0 35.0 98-99 28.48121387283237 35.0 30.0 35.0 2.0 35.0 100-101 28.378806630107206 35.0 30.0 35.0 2.0 35.0 102-103 27.578727582629316 33.5 28.0 35.0 2.0 35.0 104-105 28.034733955832223 34.0 29.0 35.0 2.0 35.0 106-107 27.883628526258583 35.0 29.0 35.0 2.0 35.0 108-109 27.534448643841706 34.5 29.0 35.0 2.0 35.0 110-111 27.049524480015812 34.0 26.0 35.0 2.0 35.0 112-113 26.41627883997826 34.0 24.5 35.0 2.0 35.0 114-115 25.65475890519243 34.0 13.5 35.0 2.0 35.0 116-117 24.904418012944024 34.0 2.0 35.0 2.0 35.0 118-119 24.227880292475668 34.0 2.0 35.0 2.0 35.0 120-121 23.489773232547797 33.0 2.0 35.0 2.0 35.0 122-123 22.677859295489355 33.0 2.0 35.0 2.0 35.0 124-125 20.388767847438366 28.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.028696033927445797 1101 2 0.08641542948756609 1101 3 0.09571818014882894 1101 4 0.21375500277815718 1101 5 0.1640874644907555 1101 6 0.19378590649395022 1101 7 0.08679002089448318 1101 8 0.10770709991813732 1101 9 0.12505570129374632 1101 10-11 0.17040232222605312 1101 12-13 0.1935938222146092 1101 14-15 0.333259809609757 1101 16-17 0.2941620578267745 1101 18-19 0.2075555173697623 1101 20-21 0.21850097119955336 1101 22-23 0.28128542470054185 1101 24-25 0.36846246115297987 1101 26-27 0.3206479790171173 1101 28-29 0.3764629399047976 1101 30-31 0.35452909923234444 1101 32-33 0.44126639776949617 1101 34-35 0.61184356303405 1101 36-37 0.6881252003970175 1101 38-39 0.682494555923455 1101 40-41 0.5650126328243985 1101 42-43 0.5810500408381287 1101 44-45 0.49511528521487236 1101 46-47 0.4210413947112457 1101 48-49 0.38668320647695253 1101 50-51 0.37155125779876386 1101 52-53 0.3710615883688462 1101 54-55 0.4498951672378908 1101 56-57 0.2028864077614969 1101 58-59 0.3169830638776787 1101 60-61 0.30496640734389757 1101 62-63 0.5321779399531437 1101 64-65 0.5151159074217198 1101 66-67 0.4038722141973139 1101 68-69 0.6635421073684107 1101 70-71 0.7071013577280958 1101 72-73 0.597543271603957 1101 74-75 0.621829096948705 1101 76-77 0.7526051360116774 1101 78-79 0.7101779701328574 1101 80-81 0.5983973802743101 1101 82-83 0.5108571591699587 1101 84-85 0.39590353850143956 1101 86-87 0.4367017692665698 1101 88-89 0.41233553856370975 1101 90-91 0.5066680363864648 1101 92-93 0.38256863252597384 1101 94-95 0.4274408486635686 1101 96-97 0.4645153407226772 1101 98-99 0.3429881674836288 1101 100-101 0.15621596480928446 1101 102-103 0.26097920582734346 1101 104-105 0.3772317453109437 1101 106-107 0.2653187973062394 1101 108-109 0.2415885517425167 1101 110-111 0.36159747536219555 1101 112-113 0.6984453316509587 1101 114-115 0.3665823753352946 1101 116-117 0.17894010702117313 1101 118-119 0.07183389099361648 1101 120-121 0.27816655064800244 1101 122-123 0.4928724980243935 1101 124-125 0.5680960098355747 1102 1 0.09911113711694242 1102 2 0.24371732773278865 1102 3 0.3028041805902859 1102 4 0.25908319503066224 1102 5 0.21070552541117848 1102 6 0.24908126572582034 1102 7 0.3211357056974862 1102 8 0.26757494011237526 1102 9 0.39476930906330665 1102 10-11 0.25728739285843716 1102 12-13 0.28266718608172425 1102 14-15 0.5238482518346785 1102 16-17 0.4470028728582278 1102 18-19 0.40944065697907917 1102 20-21 0.48280851175798745 1102 22-23 0.4216465879275546 1102 24-25 0.4615451093154377 1102 26-27 0.4723403280955836 1102 28-29 0.4358279382493464 1102 30-31 0.5407737190314847 1102 32-33 0.5754196369263269 1102 34-35 0.39915663554259595 1102 36-37 0.2066489560531224 1102 38-39 0.5802621174508786 1102 40-41 0.5175737463880168 1102 42-43 0.6585973590149266 1102 44-45 0.6944603492254657 1102 46-47 0.8222905669880376 1102 48-49 1.0451853033757459 1102 50-51 0.8882867864379946 1102 52-53 0.8576266060269404 1102 54-55 1.0253197202673618 1102 56-57 1.066660411734567 1102 58-59 0.9864386979136555 1102 60-61 0.9055071865073501 1102 62-63 1.045614646718402 1102 64-65 0.9509363190760602 1102 66-67 0.9794278234015934 1102 68-69 0.8447531354006372 1102 70-71 0.8547499340441753 1102 72-73 1.0235879272711017 1102 74-75 1.0217332190102866 1102 76-77 0.9373598540339678 1102 78-79 1.0196947177324596 1102 80-81 1.0115778377293374 1102 82-83 1.0580804926872318 1102 84-85 0.8979797442182509 1102 86-87 0.8148488418854853 1102 88-89 0.764464511635115 1102 90-91 0.9889426887427177 1102 92-93 0.9227890828073981 1102 94-95 1.1610450314248517 1102 96-97 1.0108281787096551 1102 98-99 0.8284064436120957 1102 100-101 0.737777740551472 1102 102-103 1.0172336893280587 1102 104-105 1.2151066349478477 1102 106-107 1.0991619851442245 1102 108-109 1.0818914294602067 1102 110-111 1.2682734079303444 1102 112-113 1.2797018844597012 1102 114-115 1.138439441332423 1102 116-117 0.9681069346340188 1102 118-119 0.9679649747592052 1102 120-121 0.5965330388943357 1102 122-123 0.4321952135083329 1102 124-125 0.8875592313343041 1103 1 0.00760933855578827 1103 2 0.10737052282377846 1103 3 0.06842256823311033 1103 4 0.0900052221148826 1103 5 -0.02896597268446044 1103 6 0.1128270338172328 1103 7 0.12801520210036443 1103 8 0.24771141916443185 1103 9 0.32414933445475924 1103 10-11 0.2536439840560689 1103 12-13 0.12459217550195945 1103 14-15 0.11083381044660712 1103 16-17 0.36051985339145176 1103 18-19 0.25652328499431576 1103 20-21 0.21049180629882613 1103 22-23 0.3699379548340289 1103 24-25 0.507057963737779 1103 26-27 0.45921479910700924 1103 28-29 0.6267531392650056 1103 30-31 0.6456986026539937 1103 32-33 0.6318362683270848 1103 34-35 0.5714349893301574 1103 36-37 0.6829370432304387 1103 38-39 0.7295625300619619 1103 40-41 0.7846733020376249 1103 42-43 0.8371442274025682 1103 44-45 1.1181868092339329 1103 46-47 0.8638029453206642 1103 48-49 0.8296489089618646 1103 50-51 0.7623941182196248 1103 52-53 0.670424162099728 1103 54-55 0.6919180592432355 1103 56-57 0.6181971235415986 1103 58-59 0.5529590110748899 1103 60-61 0.6111250451616002 1103 62-63 0.9434140542512033 1103 64-65 0.8353390909761877 1103 66-67 0.7701004323732406 1103 68-69 0.7072491679863333 1103 70-71 0.7057735269345713 1103 72-73 0.7397392709020814 1103 74-75 0.6357434813886229 1103 76-77 0.8859686140847494 1103 78-79 0.7359584714353815 1103 80-81 0.8218626980763517 1103 82-83 0.9474755942530386 1103 84-85 1.006184356998613 1103 86-87 0.99423547963411 1103 88-89 0.8867203834082851 1103 90-91 0.8400919164194036 1103 92-93 0.7370876439584748 1103 94-95 0.8896474436127484 1103 96-97 0.9289473598353446 1103 98-99 1.0564534177974494 1103 100-101 1.1716673935349675 1103 102-103 1.0637516220407122 1103 104-105 0.9052714148886025 1103 106-107 0.9972589487498134 1103 108-109 0.8042716135482308 1103 110-111 1.0440417638338602 1103 112-113 0.9999793389666856 1103 114-115 0.9891258040069886 1103 116-117 0.909819006153274 1103 118-119 0.8506749832230227 1103 120-121 0.8302269661055064 1103 122-123 0.9248224352603422 1103 124-125 0.8810658965903357 1104 1 0.10357542283122712 1104 2 0.10726774790085614 1104 3 0.14550376042221558 1104 4 0.10430378326596212 1104 5 0.2809261136464727 1104 6 0.2728732825325437 1104 7 0.24345713426891535 1104 8 0.18711275523843085 1104 9 0.16735334267674773 1104 10-11 0.309309451681969 1104 12-13 0.3016797911237461 1104 14-15 0.3854291341876177 1104 16-17 0.6027539232783994 1104 18-19 0.40581670739924647 1104 20-21 0.2898725873882384 1104 22-23 0.3591465879275546 1104 24-25 0.4089978404078707 1104 26-27 0.41538129448213823 1104 28-29 0.6817312995938849 1104 30-31 0.6695552316365223 1104 32-33 0.5313545108759001 1104 34-35 0.47483940865184593 1104 36-37 0.22644937622119699 1104 38-39 0.19635980652650886 1104 40-41 0.21878172958130193 1104 42-43 0.12561416573760908 1104 44-45 0.060452996284297456 1104 46-47 0.10343552497123909 1104 48-49 -0.2791319235150098 1104 50-51 -0.07182350767966028 1104 52-53 -0.0964437721243172 1104 54-55 0.13663799757827633 1104 56-57 0.13577805879339877 1104 58-59 0.14920130295566736 1104 60-61 0.08901558986869418 1104 62-63 0.07817767192848635 1104 64-65 0.32856236949622897 1104 66-67 0.3427944200402493 1104 68-69 0.2722478832997943 1104 70-71 0.22539069034669623 1104 72-73 0.25586208693496815 1104 74-75 0.3366491853968441 1104 76-77 0.4101014506726237 1104 78-79 0.5417010202534698 1104 80-81 0.5306692242839617 1104 82-83 0.5238630557124431 1104 84-85 0.6174125173274945 1104 86-87 0.404108295667001 1104 88-89 0.5343437133157884 1104 90-91 0.4728449996670889 1104 92-93 0.474574797093112 1104 94-95 0.5029567961307357 1104 96-97 0.46374309467604036 1104 98-99 0.7401186284860444 1104 100-101 0.7374626145010552 1104 102-103 0.6538145716810035 1104 104-105 0.6853009626789408 1104 106-107 0.6946189179173352 1104 108-109 0.40366138744339963 1104 110-111 0.26577866003118444 1104 112-113 0.14422394328323307 1104 114-115 0.22649091192065995 1104 116-117 0.11981386740713162 1104 118-119 0.4507906050113064 1104 120-121 0.20708976158340775 1104 122-123 0.07838744039909074 1104 124-125 0.10336805486371858 1105 1 -0.02604857327487764 1105 2 0.008654946732690405 1105 3 -0.06653079786447336 1105 4 0.08498947321485417 1105 5 -0.0235457773245642 1105 6 0.0022660788333510595 1105 7 0.04499643823581323 1105 8 0.08715230257354989 1105 9 0.1239881686684754 1105 10-11 0.08194573788298953 1105 12-13 0.06762497243306598 1105 14-15 0.08015869125746633 1105 16-17 0.10101536158455104 1105 18-19 -0.10155430744621441 1105 20-21 3.343785783087583E-4 1105 22-23 0.26033909218649853 1105 24-25 0.29333405359842857 1105 26-27 0.39662977343078865 1105 28-29 0.31496475055519113 1105 30-31 0.27757725646008424 1105 32-33 0.23325734611562154 1105 34-35 0.3623637454734592 1105 36-37 0.17436553587074144 1105 38-39 0.09140270017459784 1105 40-41 0.12075605423449787 1105 42-43 0.025533550016028528 1105 44-45 0.006492178567086171 1105 46-47 0.004996123657043938 1105 48-49 -0.08727411139526708 1105 50-51 -0.0945130306779518 1105 52-53 -0.30629105856871064 1105 54-55 -0.33263980724041176 1105 56-57 -0.14321470100217226 1105 58-59 -0.1470979791080893 1105 60-61 -0.14625150674848797 1105 62-63 -0.1790321114737985 1105 64-65 -0.09210719730834427 1105 66-67 -0.026122895608327923 1105 68-69 0.0719756151080233 1105 70-71 -0.18645221546980295 1105 72-73 -0.3439949342380686 1105 74-75 -0.3780031022643868 1105 76-77 -0.25183119717356917 1105 78-79 -0.2688581182230507 1105 80-81 -0.5154962052609413 1105 82-83 -0.38681472476942247 1105 84-85 -0.3305188528355636 1105 86-87 -0.19277811221812868 1105 88-89 -0.17940354879178244 1105 90-91 -0.3185139076096206 1105 92-93 -0.5193957553527397 1105 94-95 -0.5477246348743741 1105 96-97 -0.20305691749237198 1105 98-99 -0.349880458883149 1105 100-101 -0.35997441397302765 1105 102-103 -0.5729635373479596 1105 104-105 -0.4974913833906598 1105 106-107 -0.4734952826180745 1105 108-109 -0.08120263056584776 1105 110-111 -0.3050414908571071 1105 112-113 -0.327496670004674 1105 114-115 -0.2806108768357305 1105 116-117 -0.28736549496814234 1105 118-119 -0.24344461036956488 1105 120-121 -0.21860687993520855 1105 122-123 -0.372073438160168 1105 124-125 -0.1337699349148167 1106 1 0.005570167217278765 1106 2 0.07291514398303889 1106 3 0.031194156982181198 1106 4 0.21387736186127881 1106 5 0.33335086281035586 1106 6 0.3186329576400482 1106 7 0.33921680937641696 1106 8 0.21289512504253594 1106 9 0.2690315557680094 1106 10-11 0.25960209382243704 1106 12-13 0.298129265562352 1106 14-15 0.09932963585985988 1106 16-17 0.34059732987180524 1106 18-19 0.26588001365821157 1106 20-21 0.28826604175996096 1106 22-23 0.43941414645598087 1106 24-25 0.11852364069453358 1106 26-27 0.14359438573870875 1106 28-29 0.21374575253225458 1106 30-31 0.2862119444888904 1106 32-33 0.24039356964322423 1106 34-35 0.25123095189121614 1106 36-37 0.1321051335073804 1106 38-39 0.5109471213855628 1106 40-41 0.2931307501259752 1106 42-43 0.5653268986568705 1106 44-45 0.7252523273879703 1106 46-47 0.7965614208145197 1106 48-49 0.6835263421323887 1106 50-51 0.8205886996782041 1106 52-53 0.9389300931408542 1106 54-55 0.8565555799958631 1106 56-57 0.9206651825392171 1106 58-59 0.9796390717086538 1106 60-61 0.8297645148567483 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28-29 -1.4382187927209031 2216 30-31 -1.1777416758178916 2216 32-33 -1.5791881270193215 2216 34-35 -1.5109123799472783 2216 36-37 -1.5417741409724357 2216 38-39 -1.7314197342073996 2216 40-41 -1.592408554286692 2216 42-43 -1.3261354880819098 2216 44-45 -1.176986422132515 2216 46-47 -1.14680391652427 2216 48-49 -1.649465699190067 2216 50-51 -1.7187035561449235 2216 52-53 -1.585472735890761 2216 54-55 -1.7524602022832454 2216 56-57 -1.7003588555846392 2216 58-59 -1.6550668138225433 2216 60-61 -1.4520904567502768 2216 62-63 -1.676351522624941 2216 64-65 -1.7410271181566657 2216 66-67 -1.9451080147624253 2216 68-69 -1.6476964393404074 2216 70-71 -1.6768623675808385 2216 72-73 -1.4136076799701804 2216 74-75 -1.588831140012804 2216 76-77 -1.5918559114321518 2216 78-79 -1.6373867941937945 2216 80-81 -1.6822995617765066 2216 82-83 -1.6551295591040827 2216 84-85 -1.8449345862958602 2216 86-87 -2.069652666714724 2216 88-89 -2.189147516778835 2216 90-91 -2.0312615662225966 2216 92-93 -2.2727884616190934 2216 94-95 -2.2494664671972124 2216 96-97 -2.3506998432422606 2216 98-99 -2.527872659305327 2216 100-101 -2.1075105565121 2216 102-103 -2.335714911356156 2216 104-105 -2.290834220796711 2216 106-107 -2.544195125470619 2216 108-109 -2.2175493787693874 2216 110-111 -2.600595676456237 2216 112-113 -2.31668074169254 2216 114-115 -2.2797590880793415 2216 116-117 -2.616788948194067 2216 118-119 -2.7030087257064395 2216 120-121 -3.0135858673111215 2216 122-123 -2.922199623990476 2216 124-125 -3.3116561777695637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 27.0 5 70.0 6 238.0 7 647.0 8 865.0 9 1227.0 10 1737.0 11 3020.0 12 4551.0 13 4663.0 14 6082.0 15 8096.0 16 4106.0 17 2207.0 18 2474.0 19 3656.0 20 4498.0 21 5934.0 22 5513.0 23 2000.0 24 2720.0 25 3486.0 26 4463.0 27 5298.0 28 6602.0 29 8305.0 30 10481.0 31 14238.0 32 19526.0 33 26019.0 34 34676.0 35 51886.0 36 111754.0 37 41357.0 38 2387.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.54527408008792 20.172967728202057 11.545868130671327 16.735890061038695 2 19.04968936427751 20.693403159467906 37.016661931993625 23.240245544260958 3 29.33624820908058 22.033743392124894 28.269601304283388 20.36040709451114 4 16.034842654056565 22.319282174054987 26.782940751659527 34.86293442022892 5 15.60965726160149 33.06359352723302 28.27202621129646 23.054722999869043 6 31.84812815730642 34.83023109321542 9.567580252708026 23.75406049677013 7 27.303492910429327 39.39775702781483 12.11526110370041 21.18348895805543 8 36.04836717553481 27.257793587273355 12.879304382194556 23.814534854997284 9 33.25873227607332 14.394298700657082 17.219999011906527 35.12697001136307 10-11 28.828244651944075 25.11091349241638 18.25305073859987 27.80779111703967 12-13 32.414035867793096 25.27049058840966 19.75606442369448 22.55940912010276 14-15 35.04372313620869 19.711600217380564 22.717257052517166 22.527419593893583 16-17 30.350279136406304 21.827478879502003 24.28622597697742 23.536016007114274 18-19 29.178647300034584 21.471147670569636 24.521762758756978 24.828442270638803 20-21 35.02210859147276 19.069833506249694 22.55965614347117 23.348401758806382 22-23 30.94437033743392 22.67489254483474 22.388715972531 23.992021145200333 24-25 26.88034188034188 23.4068227854355 23.787856331208932 25.924979003013686 26-27 24.4017094017094 22.763203399041547 26.48399288572699 26.35109431352206 28-29 24.77755545674621 19.774344152956868 29.446791166444346 26.001309223852576 30-31 27.152685144014622 20.563707326713107 27.621041450521222 24.66256607875105 32-33 23.49439256953708 21.1201274640581 28.364952324489895 27.020527641914928 34-35 22.328689294007212 21.706313917296576 29.311792895607923 26.653203893088286 36-37 25.05014574378736 22.206289214959735 25.749962946494737 26.993602094758163 38-39 25.459339953559606 17.99367620176869 28.369028210068674 28.177955634603038 40-41 23.270836421125438 18.51971246479917 29.546465095598045 28.662986018477348 42-43 24.577343016649376 18.16696309470876 31.144212242478137 26.111481646163725 44-45 24.33649523244899 18.41435699817203 30.96561434711724 26.283533422261744 46-47 22.8617657230374 18.193765130181315 29.442221234128745 29.502247912652535 48-49 21.57489748530211 19.10207005582728 30.26715577293612 29.05587668593449 50-51 21.951237587075738 19.860802331900597 29.22731090361148 28.960649177412183 52-53 21.232029049948125 20.292352156514006 28.984857467516427 29.490761326021442 54-55 19.603280470332493 20.70216392470728 27.060298404229037 32.634257200731184 56-57 20.464897979348844 21.182624376265995 26.09419001037498 32.25828763401018 58-59 20.07003112494442 21.431500419939724 26.75880638308384 31.739662072032015 60-61 21.098389407637963 23.129045007657727 23.665456252161455 32.10710933254286 62-63 19.28215009139865 24.283755743293316 23.709055876685934 32.7250382886221 64-65 18.605800108690282 24.058099896250184 23.762412924262634 33.5736870707969 66-67 19.54844128254533 24.75248258485253 22.937972432192087 32.76110370041006 68-69 19.62637715527889 24.320809248554916 23.04085766513512 33.011955931031075 70-71 19.264240897188873 23.720171928264413 23.77686379131466 33.238723383232056 72-73 19.860061261795366 22.39007460105726 24.383306160762807 33.36655797638456 74-75 19.854503236006128 22.07141445580752 24.792500370535052 33.2815819376513 76-77 19.354527938342965 22.368583568005533 25.38189812756287 32.894990366088635 78-79 19.445926584654906 22.55706239810286 25.900647201225237 32.09636381601699 80-81 19.656884541277606 22.960451558717455 25.428709055876688 31.95395484412826 82-83 18.92408971888741 22.588434365891015 25.709698137443805 32.77777777777778 84-85 18.880860629415544 21.71286003655946 26.2592016204733 33.1470777135517 86-87 19.26201768687318 21.78894323402994 26.22869423447458 32.7203448446223 88-89 19.302035472555705 21.657279778667064 26.157922039424932 32.882762709352306 90-91 19.42270638802431 21.079862655007165 26.141742008794033 33.3556889481745 92-93 19.253371868978807 20.464650955980435 26.494367867200236 33.78760930784052 94-95 18.82750358183884 20.35571365051134 26.78289116150388 34.03389160614594 96-97 19.057605849513365 20.640037547551998 26.565263573934097 33.73709302900054 98-99 19.27486290203053 21.196951731633813 26.475717602885236 33.05246776345042 100-101 19.664295242329924 21.97519885381157 26.08616175090164 32.27434415295687 102-103 19.224840669927374 23.133985475025938 25.751074551652586 31.890099303394102 104-105 18.770070648683365 24.991354182105628 25.44711229682328 30.791462872387726 106-107 18.32468751543896 27.1034039820167 24.817449730744528 29.754458771799815 108-109 17.73010226767452 28.607405760584953 24.42184180623487 29.240650165505656 110-111 17.57531217963761 29.933673406370808 24.088904808369254 28.40210960562232 112-113 17.69613655451806 31.044785336692854 23.73474630700064 27.52433180178845 114-115 17.380370689676468 31.868171229678886 23.47133120724878 27.280126873395865 116-117 16.950883902014844 32.28107491180743 23.600529649502466 27.167511536675264 118-119 17.092632026205145 32.503100250490355 23.906883791248156 26.497383932056344 120-121 17.33627129362476 33.07119777874392 23.604921742223627 25.98760918540769 122-123 17.051395906953534 33.3074774862682 23.492589705996785 26.148536900781483 124-125 16.637694481200203 34.74327184311284 23.29820841935416 25.320825256332803 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 1.5 5 2.0 6 1.0 7 0.5 8 0.5 9 1.5 10 2.5 11 6.5 12 10.0 13 10.5 14 12.5 15 11.5 16 16.0 17 21.0 18 26.5 19 38.0 20 57.5 21 91.0 22 143.5 23 211.5 24 287.0 25 423.0 26 573.5 27 736.0 28 1000.0 29 1257.5 30 1500.5 31 1772.5 32 2039.5 33 2221.5 34 2363.5 35 2410.5 36 2341.0 37 2354.0 38 2346.0 39 2325.5 40 2406.0 41 2475.0 42 2616.0 43 2858.0 44 3117.5 45 3442.5 46 3878.5 47 4436.0 48 5551.5 49 7915.5 50 12747.5 51 21228.5 52 32161.0 53 41033.5 54 43964.5 55 42133.5 56 37707.0 57 30580.0 58 22213.5 59 15092.0 60 10144.0 61 6841.5 62 4803.0 63 3829.5 64 3176.0 65 2742.0 66 2295.0 67 1823.0 68 1578.5 69 1345.5 70 882.5 71 450.0 72 250.5 73 184.0 74 129.0 75 59.5 76 29.5 77 22.0 78 21.0 79 13.5 80 9.0 81 6.5 82 3.5 83 2.0 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2010770218862704 2 0.0012351168420532581 3 0.0 4 0.12054740378439802 5 0.02692554715676103 6 0.0012351168420532581 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0012351168420532581 112-113 0.0 114-115 0.0017291635788745617 116-117 0.005928560841855639 118-119 0.0034583271577491234 120-121 9.880934736426065E-4 122-123 0.0028407687367224942 124-125 0.030013339261894176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 404820.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.77522844712204 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49889284998156 49.313901667422904 2 8.90701761056352 10.64838024907947 3 3.0185203757328374 5.412982350951691 4 1.5749758582017481 3.7657816689084633 5 1.0015389894691253 2.993361094710836 6 0.6456247039097592 2.315541850038683 7 0.4360574673797533 1.8245804310088753 8 0.3519217139879686 1.6828960679306855 9 0.2499755693724585 1.34481120888943 >10 1.2641899266807306 12.29439685465267 >50 0.027710408277012044 1.1337659683111 >100 0.018197880061671997 2.017094752534225 >500 0.0037222936489783626 1.5800039911075172 >1k 0.0012407645496594541 1.8111577591697186 >5k 4.135881832198181E-4 1.861344085283758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 7529 1.859838940763796 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 3968 0.9801887258534657 TruSeq Adapter, Index 11 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 2340 0.5780346820809248 RNA PCR Primer, Index 11 (95% over 23bp) CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 1018 0.25146978904204337 TruSeq Adapter, Index 11 (96% over 26bp) TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 967 0.23887159725310014 TruSeq Adapter, Index 11 (96% over 28bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 809 0.19984190504421717 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 781 0.19292525072871894 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 715 0.17662170841361594 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 703 0.1736574279926881 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 663 0.16377649325626203 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 627 0.1548836519934786 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 603 0.14895509115162295 TruSeq Adapter, Index 11 (95% over 24bp) TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 523 0.12919322167877081 No Hit TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 479 0.11832419346870214 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 455 0.11239563262684649 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.940467368213033E-4 0.0 0.0 0.0 0.0 3 4.940467368213033E-4 0.0 0.0 0.0 0.0 4 4.940467368213033E-4 0.0 0.0 0.0 0.0 5 0.0012351168420532581 0.0 0.0 0.0 0.0 6 0.0012351168420532581 0.0 0.0 0.0 0.0 7 0.0014821402104639098 0.0 0.0 0.0 0.0 8 0.0022232103156958646 0.0 0.0 0.0 0.0 9 0.0022232103156958646 0.0 0.0 0.0 0.0 10-11 0.004199397262981078 0.0 0.0 2.4702336841065167E-4 0.0 12-13 0.004446420631391729 0.0 0.0 6.175584210266292E-4 0.0 14-15 0.005434514105034336 0.0 0.0 7.410701052319549E-4 0.0 16-17 0.007534212736524876 0.0 0.0 0.0014821402104639098 0.0 18-19 0.009016352946988784 0.0 0.0 0.0016056518946692357 0.0 20-21 0.010374981473247369 0.0 0.0 0.0037053505261597742 0.0 22-23 0.010992539894273998 0.0 0.0 0.013956820315201818 0.0 24-25 0.011363074946889975 0.0 0.0 0.03655945852477645 0.0 26-27 0.011980633367916604 0.0 0.0 0.09992095252210859 0.0 28-29 0.012845215157353885 0.0 0.0 0.3560841855639544 0.0 30-31 0.013709796946791166 0.0 0.0 0.9118867644879205 0.0 32-33 0.014327355367817795 0.0 0.0 1.8088286151869968 0.0 34-35 0.015191937157255076 0.0 0.0 3.1337384516575266 0.0 36-37 0.015809495578281707 0.0 0.0 4.938738204634158 0.0 38-39 0.015809495578281707 0.0 0.0 7.227656736327257 0.0 40-41 0.01667407736771899 0.0 0.0 9.807568796008102 0.0 42-43 0.01741514747295094 0.0 0.0 11.963341732127859 0.0 44-45 0.017909194209772245 0.0 0.0 13.879502000889286 0.0 46-47 0.019391334420236155 0.0 0.0 16.115804555110913 0.0 48-49 0.019638357788646803 0.0 0.0 18.49377501111605 0.0 50-51 0.01976186947285213 0.0 0.0 20.7258781680747 0.0 52-53 0.01976186947285213 0.0 0.0 22.703917790622995 0.0 54-55 0.01976186947285213 0.0 0.0 24.694926140012846 0.0 56-57 0.01976186947285213 0.0 0.0 26.752136752136753 0.0 58-59 0.01976186947285213 0.0 0.0 28.750061755842104 0.0 60-61 0.020008892841262782 0.0 0.0 30.750580504915767 0.0 62-63 0.020255916209673434 0.0 0.0 32.668222913887654 0.0 64-65 0.020255916209673434 0.0 0.0 34.50261844770515 0.0 66-67 0.02037942789387876 0.0 0.0 36.441751889728764 0.0 68-69 0.020502939578084085 0.0 0.0 38.506373202904996 0.0 70-71 0.020502939578084085 0.0 0.0 40.710809742601654 0.0 72-73 0.020502939578084085 0.0 0.0 42.829529173459804 0.0 74-75 0.020502939578084085 0.0 0.0 44.711229682327954 0.0 76-77 0.02062645126228941 0.0 0.0 46.65332740477249 0.0 78-79 0.020749962946494737 0.0 0.0 48.63890124005731 0.0 80-81 0.020749962946494737 0.0 0.0 50.56728916555506 0.0 82-83 0.020749962946494737 0.0 0.0 52.494812509263376 0.0 84-85 0.020749962946494737 0.0 0.0 54.5360901141248 0.0 86-87 0.020749962946494737 0.0 0.0 56.26093078405217 0.0 88-89 0.020749962946494737 0.0 0.0 57.74480015809496 0.0 90-91 0.020749962946494737 0.0 0.0 59.48792055728472 0.0 92-93 0.020749962946494737 0.0 0.0 61.10740576058495 0.0 94-95 0.020749962946494737 0.0 0.0 62.552986512524086 0.0 96-97 0.020749962946494737 0.0 0.0 63.91371473741416 0.0 98-99 0.020749962946494737 0.0 0.0 65.17896843041352 0.0 100-101 0.020749962946494737 0.0 0.0 66.40939182846697 0.0 102-103 0.020749962946494737 0.0 0.0 67.59411590336447 0.0 104-105 0.020749962946494737 0.0 0.0 68.68410651647646 0.0 106-107 0.020749962946494737 0.0 0.0 69.7032014228546 0.0 108-109 0.020749962946494737 0.0 0.0 70.68808359270787 0.0 110-111 0.020749962946494737 0.0 0.0 71.64455807519391 0.0 112-113 0.020749962946494737 0.0 0.0 72.60313225631145 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 15 2.6866214E-4 119.153366 2 GCGTTAG 25 0.0020273977 71.65154 1 GTCAGTA 25 0.0020454042 71.49202 2 TCACATC 45 3.1483828E-4 52.95705 3 AAGGTTT 35 0.007794832 50.996346 5 AGGGTCG 60 2.1917744E-5 49.57978 6 GTATGGT 25 5.333547E-4 47.59659 14-15 ATGGTTT 25 5.333547E-4 47.59659 16-17 CTACGGG 80 2.2699714E-6 44.682514 3 ATGTGTA 125 6.220944E-10 42.83693 6 TGTGTAT 130 9.131327E-10 41.189358 7 GTCAGAT 75 8.117387E-5 39.717793 3 TCATAGG 75 8.1827E-5 39.663826 9 GGGTGTA 60 0.0013079694 39.663822 7 GTACCGA 60 0.0013079694 39.663822 6 TGTGTTA 30 0.0013087441 39.663822 52-53 GTTATGT 30 0.0013087441 39.663822 14-15 TCTACGG 95 7.394623E-6 37.62738 2 GTTTAGG 80 1.1715896E-4 37.318512 1 AGATGTG 145 2.6266207E-9 36.978634 4 >>END_MODULE