FastQCFastQC Report
Thu 26 May 2016
SRR935939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371109
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8420.22688751822240905No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7920.21341438768663654No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7530.20290534586873396No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6120.16491111775785552No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6070.16356380470427825No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4660.1255695765933998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAAAC551.7259299E-764.900122
TCGGCTA150.004240541759.48376546-47
AGTCTTG300.004238873359.4837657
CATAAGT150.004240541759.48376538-39
GCTAATT150.004240541759.48376548-49
TGTAAGG602.1922799E-549.5764852
GTGGAAA751.4586185E-647.6447871
GTATGAT505.335082E-447.5870133
GTGATAG653.4960736E-545.8122941
TATGATC653.5212277E-545.756744
TATATGG1056.37192E-945.3270722
TAACAAC1204.129106E-1044.6669851
GTATTAG705.414673E-542.539991
ACTAGGG705.4535838E-542.4884073
GTATATG1151.4098987E-841.430251
GTACTAG758.132721E-539.7039871
TACTAGG758.184591E-539.6611822
ATATGGG1351.3242243E-939.655843
TAAGAGG957.4725795E-637.5686954
GTCCTAG650.001928284236.6498371