##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935939_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 371109 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.431231794432364 31.0 31.0 34.0 23.0 34.0 2 29.428283873471138 31.0 31.0 34.0 22.0 34.0 3 29.43557283709099 31.0 31.0 34.0 20.0 34.0 4 32.67685504797782 37.0 35.0 37.0 22.0 37.0 5 32.77304242149881 37.0 35.0 37.0 23.0 37.0 6 32.95014672239154 37.0 35.0 37.0 25.0 37.0 7 32.926919045347866 37.0 35.0 37.0 25.0 37.0 8 32.82284989046345 37.0 35.0 37.0 22.0 37.0 9 34.17249649024949 39.0 35.0 39.0 17.0 39.0 10-11 34.116432099464035 39.0 35.0 39.0 18.5 39.0 12-13 34.02313067050382 39.0 35.0 39.0 18.0 39.0 14-15 35.015173439609384 40.0 36.0 41.0 17.0 41.0 16-17 34.97306721205899 40.0 36.0 41.0 19.0 41.0 18-19 35.07122031532515 40.0 36.0 41.0 21.5 41.0 20-21 35.17371311393688 40.0 36.0 41.0 23.0 41.0 22-23 34.92577113462622 40.0 35.0 41.0 20.0 41.0 24-25 34.66942731111345 40.0 35.0 41.0 18.5 41.0 26-27 34.50506185514229 40.0 35.0 41.0 16.0 41.0 28-29 34.38342777997839 40.0 35.0 41.0 15.0 41.0 30-31 34.24143715188799 40.0 35.0 41.0 9.0 41.0 32-33 33.95747745271605 39.0 34.0 41.0 5.0 41.0 34-35 33.824660679207454 39.0 34.0 41.0 2.0 41.0 36-37 33.746749607258245 39.0 34.0 41.0 2.0 41.0 38-39 33.640384630930534 39.0 34.0 41.0 2.0 41.0 40-41 33.41033092703222 39.0 34.0 41.0 2.0 41.0 42-43 33.16366350587025 38.5 33.5 41.0 2.0 41.0 44-45 32.84476393727988 38.0 33.0 40.5 2.0 41.0 46-47 32.510893026038175 37.0 33.0 40.0 2.0 41.0 48-49 32.14172790204495 37.0 33.0 40.0 2.0 41.0 50-51 31.75839308666726 36.0 32.5 39.0 2.0 41.0 52-53 31.384634972474394 35.5 32.0 39.0 2.0 41.0 54-55 30.975716029522324 35.0 32.0 38.5 2.0 40.5 56-57 30.607405102005075 35.0 31.5 37.0 2.0 40.0 58-59 30.24429615018768 35.0 31.0 36.5 2.0 39.0 60-61 29.931250656815116 35.0 31.0 36.0 2.0 39.0 62-63 29.592984810392636 35.0 31.0 35.5 2.0 37.5 64-65 29.347500330091698 35.0 31.0 35.0 2.0 37.0 66-67 29.17697630615264 35.0 30.5 35.0 2.0 36.5 68-69 29.047358053833243 35.0 31.0 35.0 2.0 36.0 70-71 28.812212853905457 35.0 30.0 35.0 2.0 35.0 72-73 28.60247932548119 35.0 30.0 35.0 2.0 35.0 74-75 28.406340185767522 35.0 29.5 35.0 2.0 35.0 76-77 28.25108660797771 34.5 29.0 35.0 2.0 35.0 78-79 28.080565278664757 34.0 29.0 35.0 2.0 35.0 80-81 27.89380478511704 34.0 29.0 35.0 2.0 35.0 82-83 27.687630857780327 34.0 28.5 35.0 2.0 35.0 84-85 27.494087990320903 34.0 27.0 35.0 2.0 35.0 86-87 27.299169516233775 34.0 27.0 35.0 2.0 35.0 88-89 26.995799077898944 34.0 25.5 35.0 2.0 35.0 90-91 26.669339735764964 34.0 25.0 35.0 2.0 35.0 92-93 26.30065829715798 34.0 23.5 35.0 2.0 35.0 94-95 25.970547736648804 33.0 21.5 35.0 2.0 35.0 96-97 25.663554373512902 33.0 19.5 35.0 2.0 35.0 98-99 25.288659127102818 33.0 18.5 35.0 2.0 35.0 100-101 24.9885128089052 33.0 16.0 35.0 2.0 35.0 102-103 24.21672204123317 31.5 15.0 34.0 2.0 35.0 104-105 24.645027471713163 32.0 15.0 35.0 2.0 35.0 106-107 24.672201428690762 32.5 14.0 35.0 2.0 35.0 108-109 24.475659442374074 32.0 7.5 35.0 2.0 35.0 110-111 24.13443085454678 32.0 3.5 35.0 2.0 35.0 112-113 23.728939476003006 32.0 2.0 35.0 2.0 35.0 114-115 23.179836651765385 31.5 2.0 35.0 2.0 35.0 116-117 22.54026579791921 31.0 2.0 35.0 2.0 35.0 118-119 21.69544662080413 31.0 2.0 35.0 2.0 35.0 120-121 20.77148762223498 30.0 2.0 35.0 2.0 35.0 122-123 19.795054822168147 29.0 2.0 34.0 2.0 35.0 124-125 17.115488980326536 21.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.37665937537918737 1101 2 -0.12928960755792218 1101 3 -0.21714937808106072 1101 4 -0.5667512796972218 1101 5 -0.5920700888785362 1101 6 -0.4571711885827625 1101 7 -0.576251886797472 1101 8 -0.5330225531080686 1101 9 -0.7303340214158069 1101 10-11 -0.552929413626579 1101 12-13 -0.6062404166532644 1101 14-15 -0.4889456232646694 1101 16-17 -0.3696821759670428 1101 18-19 -0.39218687771577265 1101 20-21 -0.40752607922713935 1101 22-23 -0.15697133261962648 1101 24-25 -0.18178253171621606 1101 26-27 -0.1416756411132738 1101 28-29 -0.44298843706632596 1101 30-31 -0.489162090766321 1101 32-33 -0.30144937823370554 1101 34-35 -0.8515243027193478 1101 36-37 -0.7164512823020814 1101 38-39 -0.3385109727015063 1101 40-41 -0.38593797814307607 1101 42-43 -0.289330528987513 1101 44-45 -0.1372040623623434 1101 46-47 -0.28377675929106516 1101 48-49 -0.14952807336970864 1101 50-51 0.034319202769562906 1101 52-53 0.04676103778203 1101 54-55 0.033576999584866485 1101 56-57 -0.31974666716771694 1101 58-59 -0.49958891355333535 1101 60-61 -0.20650130891413454 1101 62-63 -0.042930324571901934 1101 64-65 -0.1723437869495008 1101 66-67 -0.29586591566333453 1101 68-69 -0.21956187243220882 1101 70-71 -0.35173548418007883 1101 72-73 -0.40626673443707517 1101 74-75 -0.27523886135922737 1101 76-77 -0.2218448922265388 1101 78-79 0.007974116335830672 1101 80-81 -0.12571176328916422 1101 82-83 -0.2415151896309986 1101 84-85 -0.2105967253620804 1101 86-87 -0.39315853880385987 1101 88-89 -0.5040441101125062 1101 90-91 -0.4276473781623231 1101 92-93 -0.9346010953535675 1101 94-95 -0.7280428102375893 1101 96-97 -0.7406819204886972 1101 98-99 -0.8713094180430971 1101 100-101 -0.9322792926476815 1101 102-103 -0.8215769776591166 1101 104-105 -0.8284279720088072 1101 106-107 -0.8263467393364081 1101 108-109 -0.5855717952424335 1101 110-111 -0.6365752106617819 1101 112-113 -0.7717779702298273 1101 114-115 -0.8219269841160717 1101 116-117 -0.8437385814533798 1101 118-119 -0.9270953728096138 1101 120-121 -1.2556138842299305 1101 122-123 -1.3328456598257645 1101 124-125 -1.3761962605054077 1102 1 0.5480933060745237 1102 2 0.6671490053755385 1102 3 0.6643251611254364 1102 4 0.6725697658093921 1102 5 0.7749610472247639 1102 6 0.7401838046485167 1102 7 0.9104710122965614 1102 8 0.8320925150997809 1102 9 0.9974385496557332 1102 10-11 0.9457689098140136 1102 12-13 0.9031628948118993 1102 14-15 1.0146053503893881 1102 16-17 1.0689203440787765 1102 18-19 0.9658366565964229 1102 20-21 0.777811836007686 1102 22-23 1.1132358942886285 1102 24-25 0.992360965107693 1102 26-27 1.0190241402050546 1102 28-29 0.8039031593098045 1102 30-31 1.0817428347777778 1102 32-33 1.1114528398231585 1102 34-35 1.2589203499933461 1102 36-37 1.327045541607113 1102 38-39 1.478629504232785 1102 40-41 1.244302571685104 1102 42-43 1.353926786434755 1102 44-45 1.51293651870607 1102 46-47 1.5022900948170275 1102 48-49 1.371819422204588 1102 50-51 1.2582596380502018 1102 52-53 1.2904869567656796 1102 54-55 1.0645881419362162 1102 56-57 0.9730680782243475 1102 58-59 0.999359331786664 1102 60-61 1.0466279215346823 1102 62-63 1.15395606509799 1102 64-65 1.3223974397504925 1102 66-67 1.254118464217953 1102 68-69 1.2628186336596379 1102 70-71 1.3585113136955833 1102 72-73 1.3606810943664236 1102 74-75 1.4071624715575695 1102 76-77 1.580144069556237 1102 78-79 1.4679761990183238 1102 80-81 1.4571165195391202 1102 82-83 1.4126241418279228 1102 84-85 1.2868832288189047 1102 86-87 1.319858226790224 1102 88-89 1.4593943986868254 1102 90-91 1.3468293478607976 1102 92-93 1.523484919433482 1102 94-95 1.660335821440011 1102 96-97 1.7243186001819275 1102 98-99 2.080955499170276 1102 100-101 2.1231096483082688 1102 102-103 1.8689051633384892 1102 104-105 1.9148085165885043 1102 106-107 1.6711852819069541 1102 108-109 2.0617571644576564 1102 110-111 2.2989032856414546 1102 112-113 2.1604307145561776 1102 114-115 2.091454250914648 1102 116-117 2.1263124442677466 1102 118-119 2.1401336181307187 1102 120-121 1.8436988305466997 1102 122-123 2.1333732301044606 1102 124-125 1.8802652619354987 1103 1 -0.004141369447822285 1103 2 0.0640890516306527 1103 3 -0.03778003960989906 1103 4 0.08805534567853357 1103 5 0.2837989368845726 1103 6 -0.0466117437908693 1103 7 0.3709347494726245 1103 8 0.024909135313663455 1103 9 0.40125756169392446 1103 10-11 0.48938906439307317 1103 12-13 0.30863641672378606 1103 14-15 0.2246945002045706 1103 16-17 0.44926389702507663 1103 18-19 0.30385282611944575 1103 20-21 0.11425454094354137 1103 22-23 0.10556624278336102 1103 24-25 0.15055148083246195 1103 26-27 0.15679915058280613 1103 28-29 -0.14191312486263996 1103 30-31 -0.2587224837697306 1103 32-33 -0.14273087600439993 1103 34-35 -0.1847096881572412 1103 36-37 -0.07735067100828275 1103 38-39 -0.13995951709596 1103 40-41 0.01214340664922986 1103 42-43 0.2256834924380371 1103 44-45 0.0196079294870799 1103 46-47 0.09502566974438054 1103 48-49 0.14004966988689205 1103 50-51 0.1666310231339203 1103 52-53 0.18956976132793457 1103 54-55 0.022640287659594804 1103 56-57 -0.2828531694777219 1103 58-59 -0.2740578142332204 1103 60-61 0.052945500455003014 1103 62-63 -0.14196597328979976 1103 64-65 -0.20046128447809863 1103 66-67 -0.2087232602676039 1103 68-69 0.1589581106210325 1103 70-71 0.27798590303318704 1103 72-73 0.027122415602264027 1103 74-75 -0.09784059234960552 1103 76-77 -0.059888348558374105 1103 78-79 -0.05910478696677046 1103 80-81 -0.08696420947311623 1103 82-83 -0.0910703372090218 1103 84-85 -0.02829398361858182 1103 86-87 -0.02965055970486219 1103 88-89 0.1584712731061657 1103 90-91 0.06932237994287149 1103 92-93 0.1623787501081324 1103 94-95 0.17986971220677717 1103 96-97 0.016765574820681195 1103 98-99 0.26971787834361294 1103 100-101 0.366713989172883 1103 102-103 0.06951992148749397 1103 104-105 -0.08235094495451634 1103 106-107 -0.08285611049060293 1103 108-109 0.15222149464801404 1103 110-111 0.25336769490038336 1103 112-113 0.005624333722398234 1103 114-115 0.2104050109612956 1103 116-117 0.24286742657615434 1103 118-119 -0.24720552674267182 1103 120-121 -0.1477558335107041 1103 122-123 -0.06234916013847425 1103 124-125 -0.22420804253780702 1104 1 0.19258331898559078 1104 2 0.32374504091893996 1104 3 0.31884783143758355 1104 4 0.3119712999477642 1104 5 0.15191099785892703 1104 6 0.2773145102002772 1104 7 0.10337372362032227 1104 8 0.6010755788196889 1104 9 0.5222277954976136 1104 10-11 0.7662842132240613 1104 12-13 0.6267047038800229 1104 14-15 0.49547418915296504 1104 16-17 0.8430222655491235 1104 18-19 0.5739630331438477 1104 20-21 0.807490198580787 1104 22-23 0.7273089557301162 1104 24-25 0.6120047715936039 1104 26-27 0.6586402258737749 1104 28-29 0.7550252828003536 1104 30-31 0.45484224998245537 1104 32-33 0.4960450020468983 1104 34-35 0.5585311193409552 1104 36-37 0.5018670651128474 1104 38-39 0.5058072972000502 1104 40-41 0.7293902909833463 1104 42-43 0.7287862836626715 1104 44-45 0.6270855275919303 1104 46-47 0.7051039476308816 1104 48-49 0.6505940101896996 1104 50-51 0.9150474408906355 1104 52-53 0.7655173357434286 1104 54-55 0.8637413279315069 1104 56-57 0.9255230793635612 1104 58-59 0.9834996067164106 1104 60-61 0.9880572524376987 1104 62-63 0.748998595666837 1104 64-65 0.8113318971060011 1104 66-67 0.761819535076917 1104 68-69 0.9509423520465177 1104 70-71 1.0863080783344543 1104 72-73 0.8223124454714608 1104 74-75 0.962837644338002 1104 76-77 1.1104433024870488 1104 78-79 0.9987539008486586 1104 80-81 0.9224085374627151 1104 82-83 1.0049230709689567 1104 84-85 1.1097737885515713 1104 86-87 0.8507954182537318 1104 88-89 0.7241320002665326 1104 90-91 0.8157174772752427 1104 92-93 0.8656566366051983 1104 94-95 0.6963235179540241 1104 96-97 0.6324829504515392 1104 98-99 0.907721277251845 1104 100-101 0.8837214049726576 1104 102-103 0.7941311069854855 1104 104-105 0.6758759579191072 1104 106-107 0.44378154529269054 1104 108-109 0.7087154693107003 1104 110-111 0.5438584766492767 1104 112-113 0.5003714755495707 1104 114-115 0.4834892626865006 1104 116-117 0.3252909511409925 1104 118-119 0.42667952836083956 1104 120-121 0.5863053468374275 1104 122-123 0.4741247300664888 1104 124-125 0.5107543634772291 1105 1 0.21585914457207167 1105 2 0.399299781428752 1105 3 0.3325382488507209 1105 4 0.4378018237243779 1105 5 0.21218127956694843 1105 6 0.4571167362155961 1105 7 0.4654997234228304 1105 8 0.5628750117359402 1105 9 0.5979729745804008 1105 10-11 0.429823337770884 1105 12-13 0.41729104308639364 1105 14-15 0.6231315125740906 1105 16-17 0.7074652679895621 1105 18-19 0.6497716917509706 1105 20-21 0.47645682245754983 1105 22-23 0.5282301141608983 1105 24-25 0.5496714053449665 1105 26-27 0.6771286373339436 1105 28-29 0.44709492689181474 1105 30-31 0.4319825679443454 1105 32-33 0.6941522542298841 1105 34-35 0.6817148996171625 1105 36-37 0.47801110720682516 1105 38-39 0.5769219976472328 1105 40-41 0.6644401576763528 1105 42-43 0.7121317251274917 1105 44-45 0.7576064828882352 1105 46-47 0.6484837385716489 1105 48-49 0.4015444397344865 1105 50-51 0.7393519874352386 1105 52-53 0.8356780800055823 1105 54-55 0.8069024671773697 1105 56-57 0.6778665318032928 1105 58-59 0.8837150844350994 1105 60-61 0.6778046039308769 1105 62-63 0.49422176146125096 1105 64-65 0.4800110998031748 1105 66-67 0.5265785244828933 1105 68-69 0.49232571194720265 1105 70-71 0.30426149847015793 1105 72-73 0.4407652378652003 1105 74-75 0.490400101136661 1105 76-77 0.4926499918182543 1105 78-79 0.37582580198987614 1105 80-81 0.43132388399038746 1105 82-83 0.2795637063284637 1105 84-85 0.25978295781619565 1105 86-87 0.19770043877877796 1105 88-89 0.4367249272368632 1105 90-91 0.41992616973810826 1105 92-93 0.6412194883875628 1105 94-95 0.6257801332257849 1105 96-97 0.42750051311993786 1105 98-99 0.8005131772645377 1105 100-101 0.7817997387056757 1105 102-103 0.6609475383076919 1105 104-105 0.4840108589615788 1105 106-107 0.5862913140861572 1105 108-109 0.2582436048465375 1105 110-111 0.4911863413391444 1105 112-113 0.6155162793978377 1105 114-115 0.398886926640003 1105 116-117 0.2430179609732619 1105 118-119 -0.16642730062532252 1105 120-121 0.15783266419272834 1105 122-123 0.40418263749923966 1105 124-125 0.31358343427805835 1106 1 -0.010176780531942597 1106 2 -0.04543201506654526 1106 3 0.07470685743035332 1106 4 -1.0207251578328815 1106 5 -0.06223726127153384 1106 6 0.04974375515910623 1106 7 0.17686068599884663 1106 8 -0.11448846840990257 1106 9 -0.04526268573288661 1106 10-11 0.11271072282530525 1106 12-13 0.18381216632040775 1106 14-15 0.40063013202660613 1106 16-17 0.3160054547836353 1106 18-19 0.28345156251113934 1106 20-21 0.23141736109401734 1106 22-23 0.3001876790466298 1106 24-25 0.29417065247496765 1106 26-27 0.3575704811280005 1106 28-29 0.25703040987764325 1106 30-31 0.2614909558421701 1106 32-33 0.2864791541547689 1106 34-35 0.23559569577290063 1106 36-37 0.24367989242793442 1106 38-39 0.47941247101209683 1106 40-41 0.43363834255652023 1106 42-43 0.28335082573082104 1106 44-45 0.2550837681659388 1106 46-47 0.239512409370235 1106 48-49 0.10187131221992018 1106 50-51 0.14505630674814896 1106 52-53 0.4235340640358203 1106 54-55 0.2916632808943511 1106 56-57 0.2856435588071413 1106 58-59 0.4144369821476772 1106 60-61 0.570379144920274 1106 62-63 0.4264377373968493 1106 64-65 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86-87 -3.055731626971273 2216 88-89 -3.0627185215604236 2216 90-91 -3.4059748131523193 2216 92-93 -3.1436717216037557 2216 94-95 -3.2325915869724717 2216 96-97 -3.16326808759133 2216 98-99 -3.193151239861834 2216 100-101 -3.2679520884624225 2216 102-103 -3.604091595292598 2216 104-105 -4.063968055098513 2216 106-107 -3.6059743743571815 2216 108-109 -4.097079334863917 2216 110-111 -4.480949345144012 2216 112-113 -4.47539583451664 2216 114-115 -4.474294656065602 2216 116-117 -4.57472027094407 2216 118-119 -4.290678231710981 2216 120-121 -3.693552413234393 2216 122-123 -3.5083688172345973 2216 124-125 -2.9432911981881276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23661.0 3 2392.0 4 1587.0 5 2234.0 6 4490.0 7 3836.0 8 2603.0 9 2332.0 10 2049.0 11 1585.0 12 1527.0 13 1388.0 14 1437.0 15 1585.0 16 1644.0 17 1774.0 18 1989.0 19 2287.0 20 2617.0 21 3056.0 22 3513.0 23 4027.0 24 4413.0 25 4808.0 26 5194.0 27 5698.0 28 6727.0 29 8394.0 30 10683.0 31 14453.0 32 18897.0 33 25544.0 34 36368.0 35 56171.0 36 81058.0 37 18194.0 38 892.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.22239192204842 18.3351367687654 11.456627152991588 13.985844156194599 2 20.04522228450692 23.1787115691432 34.62038075115346 22.15568539519641 3 32.05067398228944 22.770414845396385 24.490538377376186 20.688372794937994 4 17.07814660202286 21.67445069382497 28.312209109602517 32.93519359454965 5 16.56772684525237 33.18800003233621 25.48767047245898 24.756602649952438 6 31.9496428273095 35.62861585140754 9.186788787121843 23.23495253416112 7 28.754779618816134 40.212930502523534 12.208069370828358 18.824220507831974 8 36.23284865861151 27.812119383036745 10.778802936072518 25.17622902227923 9 37.02604895057786 16.8565030759157 14.918258517039467 31.199189456466968 10-11 30.35024750140794 27.317445817805552 14.776386452497784 27.55592022828872 12-13 32.638254528992825 28.54942887399659 17.28360131389969 21.52871528311089 14-15 36.41692871905558 21.99192151093075 20.802378815927398 20.788770954086267 16-17 30.407643037490335 23.261359870011237 21.967400413355644 24.363596679142784 18-19 29.326424311994586 23.882336456404992 22.146323586870704 24.644915644729714 20-21 38.42065269233568 21.998253882282565 17.833843964980638 21.74724946040112 22-23 33.712601957915325 25.25295802580913 17.09821103772746 23.936228978548083 24-25 27.609408556515742 26.671005014699183 18.06935428674594 27.650232142039133 26-27 24.77556921145065 25.878759544118857 21.06974970729348 28.275921537137013 28-29 27.038582080904604 23.69589763904721 23.31457721940611 25.950943060642075 30-31 28.620701734530822 24.835695173116253 20.49734714059751 26.046255951755416 32-33 25.120921346558557 24.272922510637038 22.647658774106798 27.958497368697603 34-35 23.67943111053626 25.138705878865782 22.430202447259433 28.751660563338532 36-37 25.132777701430037 26.64351982840621 19.888765834296663 28.33493663586709 38-39 26.036959491685728 22.854067133914835 22.057400925334605 29.051572449064828 40-41 24.017337224373435 23.46951973678892 23.145356216098236 29.36778682273941 42-43 24.259449380101263 23.016418356870894 25.209844008094656 27.514288254933188 44-45 24.85590486891991 22.317432344675016 26.028606150753554 26.79805663565152 46-47 22.166533282674365 22.883573289788174 25.537375811419288 29.412517616118176 48-49 21.130045350557385 23.51883139454985 26.92106631744312 28.430056937449642 50-51 22.386684235628884 23.89661797477291 25.458557997785018 28.258139791813186 52-53 21.827684049699684 23.721602009113226 24.73047002363187 29.720243917555216 54-55 20.30292986696631 23.45847176974959 24.42624673613413 31.812351627149976 56-57 20.71327041355941 22.532285119540834 25.731627628113145 31.022816838786603 58-59 22.424543732434408 21.01646686014082 25.55340883675686 31.005580570667917 60-61 22.320935358614317 21.205360150252353 25.01946867362419 31.45423581750914 62-63 21.495706113298247 20.701465068214457 25.459501116922524 32.34332770156477 64-65 20.91680005173724 20.542782924555247 25.49770146534952 33.04271555835799 66-67 21.794270685970968 21.798043162520983 24.25958411140662 32.14810204010143 68-69 21.22722036204887 21.340941704337986 24.708925912701353 32.72291202091179 70-71 20.23906261580859 20.77729039908903 26.012007034424204 32.97163995067818 72-73 20.907873428022494 19.583464696356057 27.33698185708242 32.17168001853903 74-75 21.18129758135148 18.986458263154347 27.84288890386041 31.989355251633768 76-77 20.672823005701844 19.264607607488927 27.84122142341313 32.2213479633961 78-79 21.21272955093572 19.33455491033535 29.02898101615446 30.423734522574474 80-81 22.16681734219153 20.16927755240073 28.502599658049643 29.161305447358103 82-83 21.39317558992102 20.19864783661943 28.716630423945528 29.691546149514025 84-85 21.233429596758217 19.980567635275268 28.889134451210886 29.896868316755633 86-87 22.090496189017202 19.561652768739297 28.877373527513583 29.470477514729915 88-89 22.683782705721278 18.681879925398075 28.95258142067077 29.681755948209876 90-91 23.6827993931702 18.845137142995725 28.30058015300087 29.171483310833207 92-93 24.197649646072435 19.093357912197938 28.09038122843117 28.61861121329846 94-95 23.590565229226012 19.37310842386398 27.716390919895538 29.31993542701447 96-97 23.537305891467646 19.74938525280426 26.95833192980092 29.754976925927174 98-99 23.36284020479655 20.06871463217462 26.917003503098897 29.65144165992994 100-101 23.097785813794943 21.100922375487393 27.02037417779778 28.780917632919884 102-103 22.699799789280238 22.31877965772859 27.381173725239755 27.60024682775142 104-105 21.868162367828234 23.75790874893562 27.361037757202755 27.012891126033388 106-107 20.869699950148878 25.16080354616618 27.081284272645206 26.88821223103973 108-109 20.669668480150037 26.88185950758403 26.731499370804805 25.716972641461133 110-111 20.49734714059751 29.187381604865415 25.63384342605542 24.68142782848166 112-113 20.052303326960466 31.073457745729304 24.797058510473466 24.077180416836764 114-115 19.49326905327493 32.18835602133746 24.836122230920452 23.482252694467164 116-117 19.05437486021627 32.93439393816911 24.44847740151815 23.562753800096466 118-119 18.858149917545486 33.87956921392463 24.327520391143185 22.9347604773867 120-121 19.263141584872105 34.177080456208195 23.79895042474788 22.760827534171828 122-123 19.690718593533575 34.39822574820931 23.43701998997537 22.474035668281747 124-125 19.695635497306963 36.1997385296072 22.567487993430827 21.53713797965501 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 3.5 9 3.0 10 3.0 11 7.0 12 8.5 13 8.0 14 11.5 15 17.0 16 26.5 17 31.0 18 39.0 19 57.0 20 80.0 21 115.0 22 165.5 23 231.5 24 307.0 25 398.0 26 515.0 27 591.5 28 692.0 29 802.5 30 905.5 31 979.5 32 1035.5 33 1145.0 34 1190.5 35 1217.5 36 1264.0 37 1289.0 38 1253.0 39 1166.5 40 1141.5 41 1153.0 42 1155.0 43 1186.5 44 1278.0 45 1556.5 46 2071.0 47 2842.5 48 4318.5 49 7825.0 50 14358.5 51 23555.5 52 33869.0 53 41230.5 54 42638.5 55 39770.0 56 33863.0 57 26484.5 58 19942.0 59 14242.0 60 9884.0 61 7016.5 62 5146.5 63 4120.0 64 3307.5 65 2689.5 66 2287.0 67 1866.5 68 1371.0 69 892.0 70 654.0 71 471.5 72 303.5 73 222.5 74 166.0 75 128.0 76 109.5 77 87.5 78 67.0 79 53.5 80 46.0 81 36.0 82 27.0 83 25.5 84 21.0 85 17.5 86 12.5 87 8.0 88 5.0 89 4.0 90 4.0 91 2.5 92 1.5 93 2.0 94 3.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1401205575720341 2 0.01428151836791886 3 0.008353340932178956 4 0.013473130535772508 5 0.002155700885723601 6 0.0 7 5.389252214309003E-4 8 2.6946261071545015E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.431191375040757E-4 28-29 0.008083878321463504 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.041939160731753E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.083878321463506E-4 66-67 0.0 68-69 0.00714075918395943 70-71 0.02061388971973194 72-73 0.0 74-75 0.009161728764325305 76-77 2.6946261071545015E-4 78-79 0.0010778504428618006 80-81 6.736565267886254E-4 82-83 0.0 84-85 0.021152814941162838 86-87 0.002829357412512227 88-89 0.01967077058222786 90-91 0.0 92-93 0.015763562726853836 94-95 0.015089906200065211 96-97 0.005793446130382179 98-99 0.002425163496439052 100-101 5.389252214309003E-4 102-103 0.0 104-105 2.6946261071545015E-4 106-107 0.0010778504428618006 108-109 0.0 110-111 0.0 112-113 0.001212581748219526 114-115 0.00660183396252853 116-117 0.0 118-119 0.08043458929856187 120-121 0.0017515069696504263 122-123 0.006197640046455354 124-125 0.24116903659032793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 371109.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.40218132059019 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27278281755146 58.32844351997463 2 7.876670917133774 10.775629445528077 3 2.5400879589432783 5.212426714136579 4 1.3030308752213207 3.565206167728644 5 0.796616099161801 2.724513942888338 6 0.5154768214254257 2.1155843403422074 7 0.34775257167587004 1.6650924123732074 8 0.25712252234725497 1.4070193116163547 9 0.19672978284357628 1.2111071649503975 >10 0.8602498776749407 9.433485475600772 >50 0.018512335682902146 0.8729277915267958 >100 0.01299802292542678 1.717027412073125 >500 0.0019693974129434515 0.9715363012608956 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 842 0.22688751822240905 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 792 0.21341438768663654 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 753 0.20290534586873396 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 612 0.16491111775785552 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 607 0.16356380470427825 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 466 0.1255695765933998 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 2.6946261071545015E-4 0.0 0.0 0.0 0.0 5 0.0010778504428618006 0.0 0.0 0.0 0.0 6 0.0013473130535772509 0.0 0.0 0.0 0.0 7 0.0016167756642927011 0.0 0.0 0.0 0.0 8 0.0016167756642927011 0.0 0.0 0.0 0.0 9 0.0016167756642927011 0.0 0.0 5.389252214309003E-4 0.0 10-11 0.0024251634964390515 0.0 0.0 5.389252214309003E-4 0.0 12-13 0.004041939160731753 0.0 0.0 5.389252214309003E-4 0.0 14-15 0.004850326992878103 0.0 0.0 8.083878321463506E-4 0.0 16-17 0.004985058298235828 0.0 0.0 0.0010778504428618006 0.0 18-19 0.005119789603593553 0.0 0.0 0.0022904321910813263 0.0 20-21 0.0053892522143090035 0.0 0.0 0.006062908741097629 0.0 22-23 0.005658714825024454 0.0 0.0 0.021287546246520565 0.0 24-25 0.005793446130382179 0.0 0.0 0.05241047778415506 0.0 26-27 0.0064671026571708045 0.0 0.0 0.12260548787552983 0.0 28-29 0.00660183396252853 0.0 0.0 0.38250217591058155 0.0 30-31 0.007006027878601705 0.0 0.0 0.9499904340773195 0.0 32-33 0.0075449531000326055 0.0 0.0 1.8493218973401344 0.0 34-35 0.008218609626821232 0.0 0.0 3.1680719141815477 0.0 36-37 0.008622803542894405 0.0 0.0 4.928066956069511 0.0 38-39 0.008757534848252129 0.0 0.0 7.2691581179653415 0.0 40-41 0.009026997458967581 0.0 0.0 9.994907156657478 0.0 42-43 0.009431191375040756 0.0 0.0 12.241147479581471 0.0 44-45 0.009431191375040756 0.0 0.0 14.238539081509746 0.0 46-47 0.009431191375040756 0.0 0.0 16.543252790958988 0.0 48-49 0.009431191375040756 0.0 0.0 18.989030177117776 0.0 50-51 0.009700653985756206 0.0 0.0 21.315705089340327 0.0 52-53 0.009700653985756206 0.0 0.0 23.427887763433382 0.0 54-55 0.009700653985756206 0.0 0.0 25.486582109299427 0.0 56-57 0.009700653985756206 0.0 0.0 27.637163205419434 0.0 58-59 0.009700653985756206 0.0 0.0 29.669046021519286 0.0 60-61 0.009700653985756206 0.0 0.0 31.72720144216389 0.0 62-63 0.00983538529111393 0.0 0.0 33.719742717099294 0.0 64-65 0.009970116596471656 0.0 0.0 35.62147509222358 0.0 66-67 0.009970116596471656 0.0 0.0 37.64904650655198 0.0 68-69 0.009970116596471656 0.0 0.0 39.764058538057554 0.0 70-71 0.009970116596471656 0.0 0.0 42.04034394207632 0.0 72-73 0.009970116596471656 0.0 0.0 44.24239239684297 0.0 74-75 0.009970116596471656 0.0 0.0 46.12593604574397 0.0 76-77 0.009970116596471656 0.0 0.0 48.08465976303458 0.0 78-79 0.009970116596471656 0.0 0.0 50.09080889981111 0.0 80-81 0.009970116596471656 0.0 0.0 52.02070011775516 0.0 82-83 0.009970116596471656 0.0 0.0 54.06201412523005 0.0 84-85 0.009970116596471656 0.0 0.0 56.18497530375173 0.0 86-87 0.009970116596471656 0.0 0.0 57.96935671190944 0.0 88-89 0.009970116596471656 0.0 0.0 59.49869175902498 0.0 90-91 0.009970116596471656 0.0 0.0 61.278088108884454 0.0 92-93 0.009970116596471656 0.0 0.0 62.88974398357356 0.0 94-95 0.009970116596471656 0.0 0.0 64.32867432479406 0.0 96-97 0.009970116596471656 0.0 0.0 65.72691581179653 0.0 98-99 0.009970116596471656 0.0 0.0 67.00255181092348 0.0 100-101 0.009970116596471656 0.0 0.0 68.27428060219503 0.0 102-103 0.010239579207187106 0.0 0.0 69.49305999046102 0.0 104-105 0.010239579207187106 0.0 0.0 70.61321606320514 0.0 106-107 0.010239579207187106 0.0 0.0 71.64162011700066 0.0 108-109 0.010239579207187106 0.0 0.0 72.63714973228889 0.0 110-111 0.010239579207187106 0.0 0.0 73.61085287610918 0.0 112-113 0.010239579207187106 0.0 0.0 74.57552902247049 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGAAAC 55 1.7259299E-7 64.90012 2 TCGGCTA 15 0.0042405417 59.483765 46-47 AGTCTTG 30 0.0042388733 59.483765 7 CATAAGT 15 0.0042405417 59.483765 38-39 GCTAATT 15 0.0042405417 59.483765 48-49 TGTAAGG 60 2.1922799E-5 49.576485 2 GTGGAAA 75 1.4586185E-6 47.644787 1 GTATGAT 50 5.335082E-4 47.587013 3 GTGATAG 65 3.4960736E-5 45.812294 1 TATGATC 65 3.5212277E-5 45.75674 4 TATATGG 105 6.37192E-9 45.327072 2 TAACAAC 120 4.129106E-10 44.666985 1 GTATTAG 70 5.414673E-5 42.53999 1 ACTAGGG 70 5.4535838E-5 42.488407 3 GTATATG 115 1.4098987E-8 41.43025 1 GTACTAG 75 8.132721E-5 39.703987 1 TACTAGG 75 8.184591E-5 39.661182 2 ATATGGG 135 1.3242243E-9 39.65584 3 TAAGAGG 95 7.4725795E-6 37.568695 4 GTCCTAG 65 0.0019282842 36.649837 1 >>END_MODULE