FastQCFastQC Report
Thu 26 May 2016
SRR935939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371109
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT70061.887855050672444No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT38351.0333891120937515RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT22760.6132969019883646RNA PCR Primer, Index 46 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8800.23712709742959615No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT8370.22554020516883178No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7840.21125868680091295No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7000.1886238275008151No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6250.16841413169715636No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC6170.16625843081143277No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG5320.1433541089006195TruSeq Adapter, Index 4 (95% over 23bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC5060.1363480810220178No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4110.11074913300405002No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT4070.10967128256118822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA454.2051397E-879.574541
GTCAGAT701.72804E-1068.0779343
CAGATGT701.7462298E-1068.01375
GGGCTCG300.00423543459.495967
AATATTT150.00423710159.4959616-17
TAGGGTG201.7721666E-459.4959618-19
GTATTAG551.2856448E-554.2553671
TATTAGG551.3001209E-554.15292
CTATACA453.120984E-453.0496941
ATTAGGG602.1756967E-549.640163
GTCCAAT505.248738E-447.744721
TACACCG505.322615E-447.6095925
ATGTGTA1004.136382E-947.5967678
TGTGTAT1056.368282E-945.3302549
TTGGTAT502.2253153E-741.6471716-17
AGATGTG1151.4208126E-841.3940666
GATGTGT1202.0707375E-839.6639757
GTTCGAT300.001308649839.66397514-15
GTATGCG650.001908544936.726711
AGTTGTC650.001937856536.6128967
Produced by FastQC (version 0.11.5)