##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935939_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 371109 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29595078534878 33.0 31.0 34.0 30.0 34.0 2 32.39934628370641 34.0 31.0 34.0 31.0 34.0 3 32.38097162828172 34.0 31.0 34.0 30.0 34.0 4 35.89214220080893 37.0 35.0 37.0 35.0 37.0 5 35.88239573817935 37.0 35.0 37.0 35.0 37.0 6 35.96536866527085 37.0 35.0 37.0 35.0 37.0 7 35.95053205392486 37.0 35.0 37.0 35.0 37.0 8 35.97122947705391 37.0 35.0 37.0 35.0 37.0 9 37.61470080219019 39.0 37.0 39.0 35.0 39.0 10-11 37.54003540738705 39.0 37.0 39.0 35.0 39.0 12-13 37.540802028514534 39.0 37.0 39.0 35.0 39.0 14-15 38.662861854603364 40.0 38.0 41.0 34.0 41.0 16-17 38.57609354663994 40.0 38.0 41.0 34.0 41.0 18-19 38.64508810080057 40.0 38.0 41.0 34.0 41.0 20-21 38.76679897280853 40.0 38.0 41.0 34.0 41.0 22-23 38.6004853021619 40.0 38.0 41.0 34.0 41.0 24-25 38.30453990606533 40.0 38.0 41.0 33.0 41.0 26-27 38.16041917603723 40.0 38.0 41.0 33.0 41.0 28-29 38.108687744032075 40.0 37.0 41.0 33.0 41.0 30-31 37.985272521011346 40.0 37.0 41.0 33.0 41.0 32-33 37.65214128463605 40.0 36.5 41.0 33.0 41.0 34-35 37.49061461726878 40.0 36.0 41.0 32.0 41.0 36-37 37.43618855915648 40.0 36.0 41.0 32.5 41.0 38-39 37.06059405727159 40.0 35.5 41.0 31.5 41.0 40-41 36.89641183587571 39.0 35.0 41.0 31.0 41.0 42-43 36.6724789751798 39.0 35.0 41.0 31.0 41.0 44-45 36.26877817568423 39.0 35.0 41.0 31.0 41.0 46-47 35.78034081631003 38.5 35.0 40.5 29.5 41.0 48-49 35.19570530491042 37.5 35.0 40.0 28.5 41.0 50-51 34.555016181229774 37.0 34.5 39.5 27.5 41.0 52-53 34.12171221932101 36.0 34.0 39.0 27.0 41.0 54-55 33.658109881463396 35.5 34.0 38.5 26.0 41.0 56-57 33.256512237644465 35.0 34.0 37.0 26.0 40.0 58-59 32.94175161475469 35.0 34.0 37.0 26.0 39.0 60-61 32.55139864568092 35.0 33.0 36.0 26.0 39.0 62-63 32.20278678232002 35.0 33.0 35.5 25.0 37.5 64-65 31.915007450641188 35.0 33.0 35.0 24.5 37.0 66-67 31.4812251925984 35.0 33.0 35.0 22.5 36.5 68-69 30.962109514994246 35.0 33.0 35.0 18.5 36.0 70-71 30.44306389766888 35.0 32.5 35.0 7.0 35.0 72-73 30.107469773031642 35.0 32.0 35.0 2.0 35.0 74-75 29.972296548992347 35.0 32.0 35.0 2.0 35.0 76-77 29.8326987488851 35.0 31.0 35.0 2.0 35.0 78-79 29.8742795243446 35.0 32.0 35.0 2.0 35.0 80-81 29.81669671174776 35.0 32.0 35.0 2.0 35.0 82-83 29.695105750601577 35.0 31.0 35.0 2.0 35.0 84-85 29.62452540897688 35.0 31.0 35.0 2.0 35.0 86-87 29.601843393719903 35.0 31.0 35.0 2.0 35.0 88-89 29.505228921960935 35.0 31.0 35.0 2.0 35.0 90-91 29.375374889857156 35.0 31.0 35.0 2.0 35.0 92-93 29.27345065735404 35.0 31.0 35.0 2.0 35.0 94-95 29.2429286274383 35.0 31.0 35.0 2.0 35.0 96-97 29.189967098615234 35.0 31.0 35.0 2.0 35.0 98-99 29.08492248908003 35.0 31.0 35.0 2.0 35.0 100-101 28.957164876087617 35.0 30.0 35.0 2.0 35.0 102-103 28.144421180839053 33.5 28.0 35.0 2.0 35.0 104-105 28.61396516926294 34.0 30.0 35.0 2.0 35.0 106-107 28.45874796892557 34.5 30.0 35.0 2.0 35.0 108-109 28.094497842951803 34.5 29.0 35.0 2.0 35.0 110-111 27.597618219983886 34.0 28.0 35.0 2.0 35.0 112-113 26.969242190299884 34.0 26.0 35.0 2.0 35.0 114-115 26.17552524999394 34.0 22.0 35.0 2.0 35.0 116-117 25.39056584453624 34.0 8.5 35.0 2.0 35.0 118-119 24.688611701683335 34.0 2.0 35.0 2.0 35.0 120-121 23.89345313641006 33.0 2.0 35.0 2.0 35.0 122-123 23.02680748782703 33.0 2.0 35.0 2.0 35.0 124-125 20.749208453581026 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05376264067307801 1101 2 0.14063526307537444 1101 3 0.08495535277969424 1101 4 0.04667151181066487 1101 5 0.30673824344284384 1101 6 0.16039669699548398 1101 7 0.13902588993266818 1101 8 0.22984354033406618 1101 9 0.027729844535556936 1101 10-11 0.3184576692062109 1101 12-13 0.3785150126750665 1101 14-15 0.26459146135255907 1101 16-17 0.40871955813310024 1101 18-19 0.3707581406359708 1101 20-21 0.34167650442529407 1101 22-23 0.3793944951302848 1101 24-25 0.6671964732922007 1101 26-27 0.5768175197128045 1101 28-29 0.6370065855851124 1101 30-31 0.6361125552211746 1101 32-33 0.5030651715267851 1101 34-35 0.7985035792641924 1101 36-37 0.7395513735061456 1101 38-39 0.5379942038417482 1101 40-41 0.7462048556578296 1101 42-43 0.6124336183381729 1101 44-45 0.526309708517438 1101 46-47 0.6599333469692183 1101 48-49 0.4620079078094008 1101 50-51 0.4066798699072933 1101 52-53 0.4071400562453036 1101 54-55 0.35365074342468006 1101 56-57 0.14779378968616896 1101 58-59 0.07864322820440606 1101 60-61 -0.009299674272149616 1101 62-63 0.14786856707141283 1101 64-65 -0.08388434107220988 1101 66-67 -0.3477073661702086 1101 68-69 -0.23037036155500346 1101 70-71 -0.252713572347929 1101 72-73 -0.4355923007613569 1101 74-75 -0.1259927051035774 1101 76-77 -0.06043097725822122 1101 78-79 -0.2282462247327679 1101 80-81 -0.12717384451301328 1101 82-83 -0.29141478848129765 1101 84-85 -0.3056652495762009 1101 86-87 -0.41595763868307856 1101 88-89 -0.3284497919734193 1101 90-91 -0.1599214685580641 1101 92-93 -0.3269883365945745 1101 94-95 -0.08731788280212882 1101 96-97 0.12317046159718359 1101 98-99 -0.2153701391830296 1101 100-101 -0.30808769419643767 1101 102-103 -0.18806108020588397 1101 104-105 -0.3584820774947026 1101 106-107 -0.23859803746516306 1101 108-109 -0.4124840240124783 1101 110-111 -0.6353390612938092 1101 112-113 -0.26717525221962646 1101 114-115 -0.03300442875528731 1101 116-117 -0.5562533390978999 1101 118-119 -0.7818345258180095 1101 120-121 -0.45993445298518054 1101 122-123 -0.20526195153298943 1101 124-125 -0.26059556430650943 1102 1 0.27096559808222054 1102 2 0.19428516414795638 1102 3 0.305844658205082 1102 4 0.18096287909172304 1102 5 0.25235940349698893 1102 6 0.25680406969151903 1102 7 0.25890509434795206 1102 8 0.20374752867104462 1102 9 0.30164424628500797 1102 10-11 0.2534467351231129 1102 12-13 0.2112860217347432 1102 14-15 0.37606704190030626 1102 16-17 0.19360969663149064 1102 18-19 0.3432771451137313 1102 20-21 0.33252311485953356 1102 22-23 0.22126682669333775 1102 24-25 0.14553657534364106 1102 26-27 0.37886896197043285 1102 28-29 0.41188943469476413 1102 30-31 0.3700602798905521 1102 32-33 0.39903518089885637 1102 34-35 0.41262416658065604 1102 36-37 0.5804344308840541 1102 38-39 0.5409828532221468 1102 40-41 0.28202699457275315 1102 42-43 0.10526919055518391 1102 44-45 0.14138832978162696 1102 46-47 0.26405705830942594 1102 48-49 0.4404833842230218 1102 50-51 0.5818751213911995 1102 52-53 0.5371619244114996 1102 54-55 0.6071756835475597 1102 56-57 0.6185112738057157 1102 58-59 0.5337301801985816 1102 60-61 0.5366338881562669 1102 62-63 0.6225535613440343 1102 64-65 0.6171257599378848 1102 66-67 0.8471796483044294 1102 68-69 0.8793141120469912 1102 70-71 0.8947299348893978 1102 72-73 0.8675629632186492 1102 74-75 0.8997804907727094 1102 76-77 0.9952495392470411 1102 78-79 0.9622359162648344 1102 80-81 0.9056388181965538 1102 82-83 0.8913822541095584 1102 84-85 1.040726503001121 1102 86-87 0.7743891279523503 1102 88-89 1.0761841765780744 1102 90-91 0.8704336288073442 1102 92-93 0.9968271377363678 1102 94-95 1.0065798574873668 1102 96-97 1.0117365346993417 1102 98-99 1.1917005678876755 1102 100-101 1.000492957683182 1102 102-103 0.9596011086934162 1102 104-105 1.1428300124771837 1102 106-107 1.5124797507260297 1102 108-109 1.305489525919839 1102 110-111 1.0739809428715539 1102 112-113 1.4162361817072657 1102 114-115 1.4493031834492314 1102 116-117 1.6187752977864065 1102 118-119 1.6948810838872888 1102 120-121 1.9648911223558585 1102 122-123 1.9082338310349556 1102 124-125 1.4936270744522133 1103 1 -0.02071836483462164 1103 2 0.07380047380025445 1103 3 0.04559045274540097 1103 4 0.07061082627392068 1103 5 -0.059182631332717506 1103 6 0.18520352411837138 1103 7 0.04564924821539762 1103 8 0.22987178493228555 1103 9 0.1907980148908308 1103 10-11 0.200905668883415 1103 12-13 -0.018987357853710307 1103 14-15 0.1340025614796616 1103 16-17 0.299235425544893 1103 18-19 0.40643712060247594 1103 20-21 0.35893597140937317 1103 22-23 0.3668498585777371 1103 24-25 0.04043269900689239 1103 26-27 0.09842261988025314 1103 28-29 0.3486978317771374 1103 30-31 0.09675976001468456 1103 32-33 0.27628122252846055 1103 34-35 0.14130826786750106 1103 36-37 0.03586545343836178 1103 38-39 0.10227285697791189 1103 40-41 0.11842225836527831 1103 42-43 0.1484010967007947 1103 44-45 0.18366036520435358 1103 46-47 0.46366566887694205 1103 48-49 0.4248218767806904 1103 50-51 0.42307182423174083 1103 52-53 0.19273329368149916 1103 54-55 0.24364408576938956 1103 56-57 0.07326032992463638 1103 58-59 0.28548649085900024 1103 60-61 0.36959757591447584 1103 62-63 0.5355781319028452 1103 64-65 0.6386205512956948 1103 66-67 0.4846205939645607 1103 68-69 0.4247449369298728 1103 70-71 0.24055412591906844 1103 72-73 0.09192399031940113 1103 74-75 -0.03427038985351416 1103 76-77 0.051038347090639036 1103 78-79 0.09200918713399986 1103 80-81 0.3263093196889706 1103 82-83 0.31202903503031365 1103 84-85 0.19610810769779263 1103 86-87 0.3642307931364819 1103 88-89 0.2857653108167426 1103 90-91 0.16428011717438196 1103 92-93 -0.0875548425360293 1103 94-95 0.2587611617234664 1103 96-97 0.17128109972335537 1103 98-99 -0.05940145033762789 1103 100-101 0.151879622768476 1103 102-103 0.0239569963369739 1103 104-105 -0.13736924032480857 1103 106-107 0.04386206703984996 1103 108-109 0.24726540605188063 1103 110-111 0.3116175832479229 1103 112-113 0.3425126449700322 1103 114-115 0.19053744386157234 1103 116-117 -0.1281479463971138 1103 118-119 0.19826917220688145 1103 120-121 -0.09329939336890192 1103 122-123 -0.45385476530021407 1103 124-125 -0.18528336062260564 1104 1 0.019218806861744042 1104 2 0.13201965188244458 1104 3 0.07762258787774812 1104 4 0.05462050801251195 1104 5 0.13190295935633856 1104 6 0.10023081838142645 1104 7 0.11627959119613962 1104 8 0.1076398351949237 1104 9 0.1552897236146933 1104 10-11 0.22015327419806852 1104 12-13 0.17624387090311444 1104 14-15 0.13714397666145572 1104 16-17 0.25852577470546123 1104 18-19 0.17247893747342147 1104 20-21 0.09948597655923663 1104 22-23 0.3757591180277302 1104 24-25 0.2157082165679114 1104 26-27 0.1375744377811685 1104 28-29 0.14746701500919102 1104 30-31 0.21671289628000778 1104 32-33 0.08478364296311014 1104 34-35 0.21269821533891786 1104 36-37 0.1713454204792555 1104 38-39 0.35322558125434966 1104 40-41 0.5272954687886013 1104 42-43 0.2525312851856967 1104 44-45 0.3854834159819802 1104 46-47 0.40127510342220774 1104 48-49 0.2995515090806222 1104 50-51 0.6094839161052619 1104 52-53 0.6922826808480451 1104 54-55 0.6803909186048998 1104 56-57 0.5116551182653524 1104 58-59 0.43721426920898665 1104 60-61 0.4176071546558475 1104 62-63 0.43782089634820665 1104 64-65 0.4441231777247836 1104 66-67 0.3132975946855403 1104 68-69 0.36668025467629306 1104 70-71 0.6577701110874656 1104 72-73 0.47623236399331503 1104 74-75 0.6495925208478965 1104 76-77 0.5775215070453221 1104 78-79 0.40832652156596794 1104 80-81 0.42656423780618624 1104 82-83 0.4745200227736426 1104 84-85 0.5845234954823226 1104 86-87 0.6197411443569969 1104 88-89 0.7717963129796814 1104 90-91 0.6926558510295671 1104 92-93 0.957892680380855 1104 94-95 0.8284774043849126 1104 96-97 0.5451610049659159 1104 98-99 0.6077501615688483 1104 100-101 0.6815041979049496 1104 102-103 0.6712172703095796 1104 104-105 0.9040227670909893 1104 106-107 0.6942637561942462 1104 108-109 0.49694353974226857 1104 110-111 0.2805124230872451 1104 112-113 0.1744726820490321 1104 114-115 0.2453577137142915 1104 116-117 0.21010779569785143 1104 118-119 0.3449114628650385 1104 120-121 0.21216460910303425 1104 122-123 0.24604996227214215 1104 124-125 0.5689137760546998 1105 1 -0.039531492902675325 1105 2 0.05221530988298184 1105 3 0.056124188972418665 1105 4 0.16538875132247455 1105 5 0.08871924961293587 1105 6 0.1301226210588453 1105 7 0.08617971674453173 1105 8 -0.0031848343588833927 1105 9 0.14259029210910512 1105 10-11 -0.01956480618110845 1105 12-13 0.1823872782018512 1105 14-15 -0.010706888776063295 1105 16-17 0.09933676089514165 1105 18-19 -0.032609299065391895 1105 20-21 0.1701034207325236 1105 22-23 0.2623000482143567 1105 24-25 -0.02354258397942033 1105 26-27 0.18202244805074486 1105 28-29 0.096334258163985 1105 30-31 0.10282886631767241 1105 32-33 0.29729545720790895 1105 34-35 0.25450829870869285 1105 36-37 0.2959081170894464 1105 38-39 0.5635120855074831 1105 40-41 0.554672776974634 1105 42-43 0.384060859590754 1105 44-45 0.5888365455225326 1105 46-47 0.5239069645007959 1105 48-49 0.6138985869065223 1105 50-51 0.5782819665785297 1105 52-53 0.6308397912600938 1105 54-55 0.47912027012628755 1105 56-57 0.3789846757425366 1105 58-59 0.3990683650489544 1105 60-61 0.3352371818815101 1105 62-63 0.22188647775012527 1105 64-65 0.3168661279709468 1105 66-67 0.1298752724634724 1105 68-69 0.07902984347003894 1105 70-71 0.23457680888260768 1105 72-73 0.5371973938286878 1105 74-75 0.5284859316732735 1105 76-77 0.6003512126413924 1105 78-79 0.6002445201270987 1105 80-81 0.759248081561914 1105 82-83 0.6356504520362947 1105 84-85 0.6255295048772922 1105 86-87 1.028439955173905 1105 88-89 0.8342337151154169 1105 90-91 0.49847198611399435 1105 92-93 0.6714836465392153 1105 94-95 0.5798510307585367 1105 96-97 0.6705119056698337 1105 98-99 0.6666186037813233 1105 100-101 0.765810296171253 1105 102-103 0.8527369701219634 1105 104-105 0.44455268005350845 1105 106-107 0.7532609207754888 1105 108-109 0.679833339288038 1105 110-111 0.27994110736830535 1105 112-113 0.21178453579220857 1105 114-115 0.6498527693646707 1105 116-117 0.7934052748554059 1105 118-119 0.667651944463028 1105 120-121 0.8180872487227191 1105 122-123 1.014936884079475 1105 124-125 1.0253950354884651 1106 1 0.06728343827574434 1106 2 0.011296225400336368 1106 3 0.09246774464535434 1106 4 0.1105475743071338 1106 5 0.1460642767265412 1106 6 0.06364025605238055 1106 7 0.09864065839796154 1106 8 0.02712204162507703 1106 9 0.13237938561246665 1106 10-11 0.07838718163576885 1106 12-13 0.2724349885509767 1106 14-15 0.2878429361259265 1106 16-17 0.1402146861980853 1106 18-19 0.4125512388850865 1106 20-21 0.2970846349969207 1106 22-23 0.276846388305799 1106 24-25 0.2855251263011169 1106 26-27 0.3448172935494753 1106 28-29 0.5033968304069205 1106 30-31 0.450711398018818 1106 32-33 0.6616245111298653 1106 34-35 0.7022258506978787 1106 36-37 0.5376685638037415 1106 38-39 0.2211243042959552 1106 40-41 0.35980292573076866 1106 42-43 0.4519443516727648 1106 44-45 0.3110926859576537 1106 46-47 0.27685413326371844 1106 48-49 0.1253374089307968 1106 50-51 -0.028467795898698967 1106 52-53 -0.1299036121238757 1106 54-55 -0.18095978447821892 1106 56-57 -0.07097776650599741 1106 58-59 -0.12764222760893063 1106 60-61 -0.1181294675211717 1106 62-63 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-0.8551392888693599 2216 36-37 -1.0552074355413978 2216 38-39 -1.3802989951119358 2216 40-41 -1.4878753874564907 2216 42-43 -1.3511607808001571 2216 44-45 -0.971701523979867 2216 46-47 -1.029786225272666 2216 48-49 -1.2486052634477147 2216 50-51 -1.3121999577585086 2216 52-53 -1.3105088128114772 2216 54-55 -1.2908945712391144 2216 56-57 -1.4097131344560054 2216 58-59 -1.1044407077857414 2216 60-61 -0.9687972892898387 2216 62-63 -1.2214555597173273 2216 64-65 -1.2700783709229597 2216 66-67 -1.9741210451977516 2216 68-69 -1.737369515270963 2216 70-71 -1.5320584715631895 2216 72-73 -1.3614962859898547 2216 74-75 -1.5895851808690828 2216 76-77 -1.7430513803971657 2216 78-79 -1.7611339766946124 2216 80-81 -1.762275398598259 2216 82-83 -1.6112447623234267 2216 84-85 -1.685206332819778 2216 86-87 -2.221964332020516 2216 88-89 -2.137501184495349 2216 90-91 -2.0899934027017757 2216 92-93 -2.0180850437802924 2216 94-95 -2.452439439964767 2216 96-97 -2.417995487010291 2216 98-99 -2.7551089451531787 2216 100-101 -2.6174872171999795 2216 102-103 -2.3735588490934028 2216 104-105 -2.216481385080481 2216 106-107 -2.322001253344528 2216 108-109 -2.386599272512246 2216 110-111 -2.2889242282425712 2216 112-113 -1.6631227056740059 2216 114-115 -1.9255686694076957 2216 116-117 -2.081701484966498 2216 118-119 -2.1036282633160894 2216 120-121 -2.42808339897687 2216 122-123 -2.7618475800912314 2216 124-125 -2.6545600203766746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 16.0 5 55.0 6 201.0 7 480.0 8 693.0 9 934.0 10 1325.0 11 2227.0 12 2904.0 13 3226.0 14 4087.0 15 4988.0 16 2618.0 17 1862.0 18 2083.0 19 2756.0 20 4801.0 21 5547.0 22 4814.0 23 2008.0 24 2551.0 25 3260.0 26 4148.0 27 5078.0 28 6435.0 29 7918.0 30 10168.0 31 13788.0 32 18650.0 33 24833.0 34 33625.0 35 50748.0 36 107016.0 37 33758.0 38 1498.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.05475383722343 20.198172172199197 11.237766108238723 16.509307882338646 2 19.241288806611642 20.70503337348391 36.95874191262264 23.094935907281805 3 29.620408020285144 22.461325378797063 27.761654931569968 20.15661166934782 4 16.299961153314918 22.5281098929558 26.778854454419886 34.39307449930939 5 15.70576058479154 33.2710698536943 27.95765005229054 23.06551950922362 6 31.89536002328207 35.09287229083188 9.242227857106286 23.769539828779767 7 27.56171367441911 39.79666351395412 11.735096696657855 20.90652611496892 8 36.259697285703126 27.279855783610746 12.667976254954743 23.79247067573139 9 34.13390674976894 14.356967898919185 16.48706983662482 35.02205551468706 10-11 29.08188699277032 25.578873053469465 17.641043467013734 27.69819648674648 12-13 32.725964608780714 25.667795715005564 19.120527931146913 22.485711745066812 14-15 36.01812405519672 19.52162302719686 21.847893745503345 22.612359172103076 16-17 31.019323163814406 21.660213037140032 23.371165883877783 23.94929791516778 18-19 29.641156641310236 21.906232400723237 23.605194161284153 24.847416796682374 20-21 36.37475782047862 19.64422851507239 20.834444866602535 23.146568797846452 22-23 32.18932981954089 23.385312670940344 20.247151106548213 24.178206402970556 24-25 27.40690740456308 24.86075519591279 21.478595237517816 26.25374216200631 26-27 24.967731852366825 23.98783106850009 24.646936614310082 26.397500464823004 28-29 26.263173353381355 20.639758130360622 27.740097922712735 25.35697059354529 30-31 28.15022001622165 23.160176659687583 23.81025520803861 24.879348116052157 32-33 26.360045161933556 20.68192902893759 26.217499440865083 26.740526368263772 34-35 23.544430342567818 21.447337574674826 26.561737926054068 28.446494156703288 36-37 24.705948926056763 24.136304967004303 23.743697943191894 27.41404816374704 38-39 25.79956293164542 18.692082380109348 26.59676806544708 28.91158662279815 40-41 23.64911656683077 19.372071278249788 27.815951647629134 29.16286050729031 42-43 25.33285368988626 18.49065907859955 29.430436879730753 26.746050351783442 44-45 25.044528696420727 18.67362419127534 29.555332799797362 26.726514312506566 46-47 23.297467859847107 18.297454386716574 28.466030195980156 29.939047557456167 48-49 21.88763948058387 19.239630405083144 29.508581036838233 29.36414907749475 50-51 22.249123572858647 19.915038438841417 28.476269775187347 29.359568213112592 52-53 21.524538612644804 20.20915687843733 28.54444381569835 29.721860693219515 54-55 19.702701901597646 20.534802443486953 26.608354957707842 33.15414069720756 56-57 20.510416077217204 20.668321167096458 26.347784613146004 32.47347814254033 58-59 20.54571567922093 20.70981840914664 27.07169591683306 31.67276999479937 60-61 21.522517643064436 22.895968569881088 23.851887181394147 31.729626605660332 62-63 19.42165239862143 24.130915714789992 24.112322794650627 32.335109091937944 64-65 18.500494463890664 24.210137722340335 23.834776305613715 33.45459150815528 66-67 19.638704531552726 25.51959127911207 22.636476075762108 32.205228113573106 68-69 20.03441037538836 24.92960289295059 22.66611696294081 32.369869768720235 70-71 19.707417497285164 24.68371826067274 23.20908412353244 32.39978011850966 72-73 20.522946088615473 23.629176333637826 23.70125758200421 32.14661999574249 74-75 20.923636990749348 23.385851596161775 23.690074883659516 32.00043652942936 76-77 20.969849828487046 23.35055199415805 23.564640038371476 32.114958138983425 78-79 21.192021751021937 23.500103743105125 24.198146636163496 31.109727869709435 80-81 20.907469234106422 24.578762573799075 23.836393081278008 30.677375110816495 82-83 19.939963730332597 24.310243082221124 24.42543834830198 31.3243548391443 84-85 20.253483477900026 22.9816576800886 24.761458223864146 32.003400618147225 86-87 20.918517201145757 22.72876701993215 24.67064932405304 31.682066454869055 88-89 20.744444354623575 22.722973573801767 24.919767507659472 31.61281456391518 90-91 20.695671622084078 22.366474539825226 24.908989003230857 32.02886483485984 92-93 20.463664314258075 21.970229770768157 25.313587113220105 32.25251880175366 94-95 19.920697153666442 21.682308971218696 25.63653805216257 32.76045582295228 96-97 20.146102627529917 22.024122292911247 25.390653419884725 32.439121659674115 98-99 20.70981840914664 22.320935358614317 25.41274935396339 31.556496878275652 100-101 20.70079141168767 23.200326588684188 25.149080189378324 30.949801810249816 102-103 20.430520413140073 24.41304306820907 24.810096225098285 30.346340293552572 104-105 20.117270128183364 26.315583831165508 24.634271871606455 28.932874169044677 106-107 19.915307901452135 28.52221315031433 23.734670945732926 27.827808002500614 108-109 19.423134442980363 29.877071156991615 23.117736298499903 27.582058101528123 110-111 19.110847035617855 31.302242485974357 22.760208673532627 26.826701804875157 112-113 18.956611013505505 32.79058926242495 22.4038554814232 25.848944242646343 114-115 18.609667601261638 33.445610480038695 22.151787025522392 25.79293489317728 116-117 18.383386262919693 33.77356736429523 22.14163391409912 25.70141245868595 118-119 18.24968775220662 34.42230453018665 22.36354400908651 24.96446370852022 120-121 18.662003141950972 34.79333453693948 21.96913558623254 24.575526734877005 122-123 18.554357770044046 34.623681122280566 21.87218321672103 24.949777890954362 124-125 17.60542974472599 36.36910080634836 21.728008711742927 24.29746073718273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.0 9 3.5 10 3.5 11 2.0 12 3.5 13 5.0 14 6.0 15 6.0 16 4.0 17 7.0 18 15.5 19 23.5 20 38.5 21 52.0 22 71.0 23 112.0 24 157.5 25 237.5 26 351.5 27 467.0 28 622.0 29 792.5 30 943.0 31 1106.5 32 1259.5 33 1390.0 34 1507.0 35 1540.0 36 1527.5 37 1534.0 38 1552.0 39 1641.5 40 1690.0 41 1837.0 42 2080.5 43 2271.0 44 2502.0 45 2761.0 46 3103.0 47 3638.0 48 4533.5 49 6780.5 50 11803.0 51 20251.5 52 30937.5 53 39749.5 54 42691.0 55 41079.5 56 36722.0 57 29181.5 58 20915.5 59 14307.0 60 9596.5 61 6526.5 62 4615.5 63 3553.5 64 2768.5 65 2203.5 66 1812.5 67 1422.0 68 1044.5 69 647.5 70 446.5 71 281.0 72 142.0 73 89.5 74 49.5 75 26.5 76 18.0 77 13.5 78 11.0 79 8.5 80 5.0 81 4.0 82 2.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19509093015798593 2 0.0016167756642927011 3 0.0 4 0.11344375911120452 5 0.02829357412512227 6 0.002155700885723601 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 8.083878321463506E-4 112-113 2.6946261071545015E-4 114-115 9.431191375040757E-4 116-117 0.004985058298235828 118-119 0.0025598948017967766 120-121 5.389252214309003E-4 122-123 0.002020969580365876 124-125 0.031122931537634492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 371109.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.47540825795096 #Duplication Level Percentage of deduplicated Percentage of total 1 82.56795093780566 50.75898492924068 2 9.129201760542003 11.224428105970487 3 3.097968601178033 5.7134665358319845 4 1.5372298945977299 3.7800734142688945 5 0.9351399679896651 2.874405565524593 6 0.6736724659245049 2.48485739249097 7 0.39497678408391274 1.699695133777976 8 0.3127550977446112 1.5381397854884264 9 0.24161369573053368 1.336797052717221 >10 1.0608983991084258 10.394433438554989 >50 0.024077312984026555 1.0027429121696765 >100 0.019699619714135894 2.186873028520918 >500 0.003502154615846381 1.475049356303612 >1k 8.755386539615953E-4 1.6445952593270154 >5k 4.3776932698079765E-4 1.8854580898126443 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 7006 1.887855050672444 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 3835 1.0333891120937515 RNA PCR Primer, Index 46 (95% over 21bp) ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 2276 0.6132969019883646 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 880 0.23712709742959615 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 837 0.22554020516883178 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 784 0.21125868680091295 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 700 0.1886238275008151 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 625 0.16841413169715636 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 617 0.16625843081143277 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 532 0.1433541089006195 TruSeq Adapter, Index 4 (95% over 23bp) TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 506 0.1363480810220178 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 411 0.11074913300405002 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 407 0.10967128256118822 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 2.6946261071545015E-4 0.0 0.0 0.0 0.0 5 5.389252214309003E-4 0.0 0.0 0.0 0.0 6 5.389252214309003E-4 0.0 0.0 0.0 0.0 7 5.389252214309003E-4 0.0 0.0 0.0 0.0 8 5.389252214309003E-4 0.0 0.0 0.0 0.0 9 8.083878321463506E-4 0.0 0.0 2.6946261071545015E-4 0.0 10-11 9.431191375040756E-4 0.0 0.0 2.6946261071545015E-4 0.0 12-13 0.0013473130535772509 0.0 0.0 2.6946261071545015E-4 0.0 14-15 0.002155700885723601 0.0 0.0 5.389252214309003E-4 0.0 16-17 0.002155700885723601 0.0 0.0 6.736565267886254E-4 0.0 18-19 0.002829357412512227 0.0 0.0 0.0016167756642927011 2.6946261071545015E-4 20-21 0.0035030139393008525 0.0 0.0 0.005119789603593553 2.6946261071545015E-4 22-23 0.0036377452446585776 0.0 0.0 0.020479158414374213 2.6946261071545015E-4 24-25 0.0037724765500163023 0.0 0.0 0.05065897081450463 2.6946261071545015E-4 26-27 0.004580864382162653 0.0 0.0 0.11950666785230216 2.6946261071545015E-4 28-29 0.004850326992878103 0.0 0.0 0.37859496805520754 2.6946261071545015E-4 30-31 0.005119789603593553 0.0 0.0 0.947295807970165 2.6946261071545015E-4 32-33 0.005254520908951278 0.0 0.0 1.8489177034240614 2.6946261071545015E-4 34-35 0.005658714825024454 0.0 0.0 3.16497309415832 2.6946261071545015E-4 36-37 0.006332371351813079 0.0 0.0 4.929144806512372 2.6946261071545015E-4 38-39 0.006871296573243979 0.0 0.0 7.284248024165407 2.6946261071545015E-4 40-41 0.00767968440539033 0.0 0.0 10.021988149034382 2.6946261071545015E-4 42-43 0.007814415710748056 0.0 0.0 12.276986006806625 2.6946261071545015E-4 44-45 0.008083878321463506 0.0 0.0 14.281114174002786 2.6946261071545015E-4 46-47 0.008083878321463506 0.0 0.0 16.589869822612762 2.6946261071545015E-4 48-49 0.008083878321463506 0.0 0.0 19.0549137854377 2.6946261071545015E-4 50-51 0.008083878321463506 0.0 0.0 21.392367202088874 2.6946261071545015E-4 52-53 0.008353340932178956 0.0 0.0 23.514115798862328 2.6946261071545015E-4 54-55 0.008353340932178956 0.0 0.0 25.585474887431992 2.6946261071545015E-4 56-57 0.00848807223753668 0.0 0.0 27.749933308003847 2.6946261071545015E-4 58-59 0.008892266153609855 0.0 0.0 29.80013958163235 2.6946261071545015E-4 60-61 0.009161728764325305 0.0 0.0 31.88066039896634 2.6946261071545015E-4 62-63 0.009161728764325305 0.0 0.0 33.9134863342037 2.6946261071545015E-4 64-65 0.009161728764325305 0.0 0.0 35.84688056608705 2.6946261071545015E-4 66-67 0.009161728764325305 0.0 0.0 37.90638329978523 2.6946261071545015E-4 68-69 0.009161728764325305 0.0 0.0 40.06397042378385 2.6946261071545015E-4 70-71 0.009161728764325305 0.0 0.0 42.387546515983175 2.6946261071545015E-4 72-73 0.009161728764325305 0.0 0.0 44.623412528394624 2.6946261071545015E-4 74-75 0.009161728764325305 0.0 0.0 46.52474070960284 2.6946261071545015E-4 76-77 0.009161728764325305 0.0 0.0 48.51162326971321 2.6946261071545015E-4 78-79 0.009161728764325305 0.0 0.0 50.544988130172 2.6946261071545015E-4 80-81 0.009161728764325305 0.0 0.0 52.50788851792869 2.6946261071545015E-4 82-83 0.009161728764325305 0.0 0.0 54.586657828293035 2.6946261071545015E-4 84-85 0.009161728764325305 0.0 0.0 56.733196985252306 2.6946261071545015E-4 86-87 0.009161728764325305 0.0 0.0 58.5530127267191 2.6946261071545015E-4 88-89 0.009161728764325305 0.0 0.0 60.11441382450978 2.6946261071545015E-4 90-91 0.009161728764325305 0.0 0.0 61.93193913378549 2.6946261071545015E-4 92-93 0.009161728764325305 0.0 0.0 63.58131977397476 2.6946261071545015E-4 94-95 0.009161728764325305 0.0 0.0 65.05514552328292 2.6946261071545015E-4 96-97 0.009161728764325305 0.0 0.0 66.48289316615873 2.6946261071545015E-4 98-99 0.009161728764325305 0.0 0.0 67.78493650113579 2.6946261071545015E-4 100-101 0.009161728764325305 0.0 0.0 69.08428521000567 2.6946261071545015E-4 102-103 0.009161728764325305 0.0 0.0 70.33162763500751 2.6946261071545015E-4 104-105 0.009161728764325305 0.0 0.0 71.4773826557696 2.6946261071545015E-4 106-107 0.009161728764325305 0.0 0.0 72.53448447760631 2.6946261071545015E-4 108-109 0.009161728764325305 0.0 0.0 73.55722981657681 2.6946261071545015E-4 110-111 0.009161728764325305 0.0 0.0 74.55235523794896 2.6946261071545015E-4 112-113 0.009161728764325305 0.0 0.0 75.54303452624431 2.6946261071545015E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 45 4.2051397E-8 79.57454 1 GTCAGAT 70 1.72804E-10 68.077934 3 CAGATGT 70 1.7462298E-10 68.0137 5 GGGCTCG 30 0.004235434 59.49596 7 AATATTT 15 0.004237101 59.49596 16-17 TAGGGTG 20 1.7721666E-4 59.49596 18-19 GTATTAG 55 1.2856448E-5 54.255367 1 TATTAGG 55 1.3001209E-5 54.1529 2 CTATACA 45 3.120984E-4 53.049694 1 ATTAGGG 60 2.1756967E-5 49.64016 3 GTCCAAT 50 5.248738E-4 47.74472 1 TACACCG 50 5.322615E-4 47.609592 5 ATGTGTA 100 4.136382E-9 47.596767 8 TGTGTAT 105 6.368282E-9 45.330254 9 TTGGTAT 50 2.2253153E-7 41.64717 16-17 AGATGTG 115 1.4208126E-8 41.394066 6 GATGTGT 120 2.0707375E-8 39.663975 7 GTTCGAT 30 0.0013086498 39.663975 14-15 GTATGCG 65 0.0019085449 36.72671 1 AGTTGTC 65 0.0019378565 36.612896 7 >>END_MODULE