##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712758 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56921563840743 33.0 31.0 34.0 31.0 34.0 2 32.66538151799068 34.0 31.0 34.0 31.0 34.0 3 32.83860300410518 34.0 31.0 34.0 31.0 34.0 4 36.10157865643038 37.0 35.0 37.0 35.0 37.0 5 36.132620047758145 37.0 35.0 37.0 35.0 37.0 6 36.23049899124247 37.0 37.0 37.0 35.0 37.0 7 36.25335808226635 37.0 37.0 37.0 35.0 37.0 8 36.25709988523454 37.0 37.0 37.0 35.0 37.0 9 38.09096214984609 39.0 39.0 39.0 35.0 39.0 10-11 37.88349902772049 39.0 38.0 39.0 35.0 39.0 12-13 37.774640200460745 39.0 38.0 39.0 35.0 39.0 14-15 39.28549591867085 41.0 39.0 41.0 36.0 41.0 16-17 39.339054068842444 41.0 39.0 41.0 36.0 41.0 18-19 39.336444487469805 41.0 39.0 41.0 35.5 41.0 20-21 39.31898484478603 41.0 39.0 41.0 35.0 41.0 22-23 39.17060699423928 41.0 39.0 41.0 35.0 41.0 24-25 38.96469488942951 41.0 38.0 41.0 35.0 41.0 26-27 38.848064841082106 41.0 38.0 41.0 35.0 41.0 28-29 38.743238939443685 40.0 38.0 41.0 35.0 41.0 30-31 38.62556084954501 40.0 38.0 41.0 35.0 41.0 32-33 38.46790565661837 40.0 38.0 41.0 35.0 41.0 34-35 38.12314558377457 40.0 37.0 41.0 34.0 41.0 36-37 37.878473479077044 40.0 37.5 41.0 33.5 41.0 38-39 37.441178492559885 40.0 37.0 41.0 33.0 41.0 40-41 37.160386133863106 40.0 37.0 41.0 32.5 41.0 42-43 36.98740315787406 40.0 37.0 41.0 32.0 41.0 44-45 36.82243342059998 40.0 36.0 41.0 32.0 41.0 46-47 36.641873539125484 40.0 35.5 41.0 31.0 41.0 48-49 36.42018258651604 40.0 35.0 41.0 31.0 41.0 50-51 36.17158418425328 40.0 35.0 41.0 30.5 41.0 52-53 35.92202051748279 39.0 35.0 41.0 30.0 41.0 54-55 35.6774494288384 39.0 35.0 41.0 30.0 41.0 56-57 35.43129294936009 38.5 35.0 41.0 29.0 41.0 58-59 35.130246872009856 37.5 35.0 40.5 29.0 41.0 60-61 34.83136281879685 37.0 35.0 40.0 29.0 41.0 62-63 34.46377522244577 36.5 35.0 39.5 28.0 41.0 64-65 34.076044744499534 36.0 35.0 39.0 27.5 41.0 66-67 33.677956613605176 35.0 34.0 38.5 27.0 41.0 68-69 33.33266129597985 35.0 34.0 37.0 26.5 40.0 70-71 33.11567460484484 35.0 34.0 37.0 26.5 39.0 72-73 32.82164774018671 35.0 34.0 36.0 26.0 39.0 74-75 32.52318528869546 35.0 34.0 36.0 26.0 38.5 76-77 32.33230212779092 35.0 34.0 35.0 26.0 37.0 78-79 32.309219959649695 35.0 34.0 35.0 26.5 37.0 80-81 32.206674635710854 35.0 34.0 35.0 27.0 36.0 82-83 32.09663448182974 35.0 34.0 35.0 27.0 36.0 84-85 31.9255673033484 35.0 34.0 35.0 26.0 36.0 86-87 31.82535376663608 35.0 33.5 35.0 25.5 35.5 88-89 31.756359100844886 35.0 34.0 35.0 25.0 35.5 90-91 31.727514107172418 35.0 34.0 35.0 25.0 35.0 92-93 31.69570316993987 35.0 34.0 35.0 25.0 36.0 94-95 31.60999946685972 35.0 33.5 35.0 25.0 35.0 96-97 31.574358337612487 35.0 33.5 35.0 25.0 35.0 98-99 31.504596931917987 35.0 33.0 35.0 25.0 35.0 100-101 31.457479256634088 35.0 33.0 35.0 24.5 35.0 102-103 30.780213620892365 34.5 32.0 35.0 23.5 35.0 104-105 31.334292284337742 35.0 33.0 35.0 24.0 35.0 106-107 31.42243089519865 35.0 33.0 35.0 24.5 35.0 108-109 31.365958712494283 35.0 33.0 35.0 24.0 35.0 110-111 31.280018603789784 35.0 33.0 35.0 23.5 35.0 112-113 31.23060211179671 35.0 33.0 35.0 23.0 35.0 114-115 31.116143908591695 35.0 33.0 35.0 21.5 35.0 116-117 30.990551491530084 35.0 33.0 35.0 19.5 35.0 118-119 30.829235869678065 35.0 33.0 35.0 15.5 35.0 120-121 30.576927231963722 35.0 33.0 35.0 2.0 35.0 122-123 30.28220868794177 35.0 33.0 35.0 2.0 35.0 124-125 28.07571012882353 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10149880465395711 1101 2 0.12600935218639364 1101 3 0.02271241449433603 1101 4 0.10330113543813724 1101 5 0.2280784691827904 1101 6 0.14264423543993132 1101 7 0.12284354714119416 1101 8 0.11409638037130776 1101 9 0.1909034328752739 1101 10-11 0.09637976353872091 1101 12-13 0.1845135543064842 1101 14-15 0.27474844045995184 1101 16-17 0.19504649882130565 1101 18-19 0.16222455699248428 1101 20-21 0.143030536859996 1101 22-23 0.16423947013273477 1101 24-25 0.3487571582435507 1101 26-27 0.2568097919568544 1101 28-29 0.22382993355914493 1101 30-31 0.34043827929892245 1101 32-33 0.30782610960275747 1101 34-35 0.4580813264404284 1101 36-37 0.5637307565149641 1101 38-39 0.7244804057665846 1101 40-41 0.5178818114441697 1101 42-43 0.3933480826767166 1101 44-45 0.5198305220980828 1101 46-47 0.5462210646395249 1101 48-49 0.40124817172985416 1101 50-51 0.5802020393616516 1101 52-53 0.603514461671196 1101 54-55 0.6210670209643823 1101 56-57 0.47450212749290444 1101 58-59 0.42992359527931967 1101 60-61 0.407207949559961 1101 62-63 0.4321842888341436 1101 64-65 0.47351306683660255 1101 66-67 0.43948094743832655 1101 68-69 0.5298891814930897 1101 70-71 0.5537886795214746 1101 72-73 0.661893080089186 1101 74-75 0.6786725356459087 1101 76-77 0.7527349526025944 1101 78-79 0.6990832463181107 1101 80-81 0.7216008057998451 1101 82-83 0.5771004633723535 1101 84-85 0.5830254628327118 1101 86-87 0.5556395278274273 1101 88-89 0.45394099642446406 1101 90-91 0.35293940823260783 1101 92-93 0.4648854040209578 1101 94-95 0.4280802208108767 1101 96-97 0.5060941031669373 1101 98-99 0.4355719661354236 1101 100-101 0.3972671575840572 1101 102-103 0.4511904961652391 1101 104-105 0.5822884913476685 1101 106-107 0.5282657349915212 1101 108-109 0.5633774126252362 1101 110-111 0.614853436797123 1101 112-113 0.6065185075241395 1101 114-115 0.625305511323468 1101 116-117 0.43957327073355756 1101 118-119 0.28244244703386556 1101 120-121 0.4631226124710963 1101 122-123 0.5004184686703468 1101 124-125 0.4309578258039686 1102 1 0.1974747711335212 1102 2 0.2165346153735257 1102 3 0.24186444659601136 1102 4 0.2948663270033336 1102 5 0.23398428803825055 1102 6 0.30266543143235225 1102 7 0.30647947178431423 1102 8 0.25874834516715595 1102 9 0.17926166224069107 1102 10-11 0.27159337939981754 1102 12-13 0.3892060521644538 1102 14-15 0.41658317581986637 1102 16-17 0.34275713933771357 1102 18-19 0.3448348690991949 1102 20-21 0.35583926225865525 1102 22-23 0.44213773364251097 1102 24-25 0.4399341127298584 1102 26-27 0.464032668244478 1102 28-29 0.4462436271958623 1102 30-31 0.4704191772654269 1102 32-33 0.5020835340760144 1102 34-35 0.5607205693674473 1102 36-37 0.6540289960937784 1102 38-39 0.6524076214635812 1102 40-41 0.6034451398132603 1102 42-43 0.5710669708272675 1102 44-45 0.48626201011230563 1102 46-47 0.6073619944854869 1102 48-49 0.7363451668987935 1102 50-51 0.6438621300001586 1102 52-53 0.6366898221846853 1102 54-55 0.7436197064667098 1102 56-57 0.66690009507073 1102 58-59 0.6371496766061142 1102 60-61 0.5860640924448788 1102 62-63 0.6165952987847234 1102 64-65 0.7030531650817409 1102 66-67 0.7663308426335433 1102 68-69 0.7747525720399366 1102 70-71 0.7451572953505234 1102 72-73 0.6931219134762898 1102 74-75 0.608314447302206 1102 76-77 0.616777724918748 1102 78-79 0.5346812959449352 1102 80-81 0.5610342189742639 1102 82-83 0.6024758268772175 1102 84-85 0.39500290790368453 1102 86-87 0.4812340091773102 1102 88-89 0.48211756416937135 1102 90-91 0.4765652476857838 1102 92-93 0.5388058934090729 1102 94-95 0.5797490847099596 1102 96-97 0.5791957050455139 1102 98-99 0.6898020836748948 1102 100-101 0.5839863299147332 1102 102-103 0.627483141882351 1102 104-105 0.4562102627514548 1102 106-107 0.6689851167180976 1102 108-109 0.6983122328118512 1102 110-111 0.6678151066652696 1102 112-113 0.5489944643885849 1102 114-115 0.45393099822233296 1102 116-117 0.49781737933738057 1102 118-119 0.4547656517311722 1102 120-121 0.35944280015819174 1102 122-123 0.3723766194054754 1102 124-125 0.35340068529719204 1103 1 0.11068364965969835 1103 2 0.03559655132583117 1103 3 0.08423225810373225 1103 4 0.17674265962592983 1103 5 0.1448666695530818 1103 6 0.14781174508505757 1103 7 0.07419076117497525 1103 8 0.1194417946719426 1103 9 0.14811012291777814 1103 10-11 0.19332797466603324 1103 12-13 0.11401418007577035 1103 14-15 0.2534660788790859 1103 16-17 0.3547086868417111 1103 18-19 0.2375961398267492 1103 20-21 0.28058858859715485 1103 22-23 0.3612078506832077 1103 24-25 0.4161251002383537 1103 26-27 0.3962487716943315 1103 28-29 0.5279517361884061 1103 30-31 0.5393320640135997 1103 32-33 0.48928575524150375 1103 34-35 0.4735786837288387 1103 36-37 0.4760770293089962 1103 38-39 0.4535212721208808 1103 40-41 0.5578984641473923 1103 42-43 0.5262816476998395 1103 44-45 0.4936909496898494 1103 46-47 0.6324784471655605 1103 48-49 0.47145379641308693 1103 50-51 0.5265136207478491 1103 52-53 0.7534487817547699 1103 54-55 0.6343172976474989 1103 56-57 0.5309687548550528 1103 58-59 0.5613692774712717 1103 60-61 0.5105695200857099 1103 62-63 0.5218787479315878 1103 64-65 0.5545377769657875 1103 66-67 0.5775246168252792 1103 68-69 0.5123712617363694 1103 70-71 0.32743603525837983 1103 72-73 0.32164732342550906 1103 74-75 0.2671201106606489 1103 76-77 0.22683586177812742 1103 78-79 0.2505157925267838 1103 80-81 0.309213159830108 1103 82-83 0.36712125339314383 1103 84-85 0.24845507154590152 1103 86-87 0.20321505973132403 1103 88-89 0.09811823910916218 1103 90-91 0.08056324331912634 1103 92-93 0.21701198525201804 1103 94-95 0.3055721788998511 1103 96-97 0.34702562021785255 1103 98-99 0.3549056511407507 1103 100-101 0.2799576551999259 1103 102-103 0.3500966928326328 1103 104-105 0.1706207386351437 1103 106-107 0.13690962572496446 1103 108-109 0.07744687818723861 1103 110-111 0.18557829483541255 1103 112-113 0.1955959891389334 1103 114-115 0.26441557431412477 1103 116-117 0.27954389577881145 1103 118-119 0.34647053942016726 1103 120-121 0.2900462065588698 1103 122-123 0.052644345523088276 1103 124-125 -0.020097190176418422 1104 1 0.08016526741584329 1104 2 0.127325777956095 1104 3 0.09720516691949399 1104 4 0.09094698590705264 1104 5 0.10281549401921808 1104 6 0.1215731567147813 1104 7 0.1046636452634786 1104 8 0.14688509506856917 1104 9 0.11902493256734914 1104 10-11 0.12315053186017622 1104 12-13 0.08775729353432382 1104 14-15 0.23749276453628454 1104 16-17 0.18648766471576295 1104 18-19 0.2583670368327802 1104 20-21 0.19894659973431317 1104 22-23 0.22942837826398943 1104 24-25 0.23728668792615792 1104 26-27 0.3060259296421748 1104 28-29 0.3258518974101321 1104 30-31 0.312988632067956 1104 32-33 0.3302415132308809 1104 34-35 0.23688046508052452 1104 36-37 0.26943726589940553 1104 38-39 0.24534792414901574 1104 40-41 0.3141168750748591 1104 42-43 0.12016906943807015 1104 44-45 0.19463305823262544 1104 46-47 0.22262315108136477 1104 48-49 0.15691169578303743 1104 50-51 0.06605201228915547 1104 52-53 0.20005372918461717 1104 54-55 0.18863665513441674 1104 56-57 0.1706382008655538 1104 58-59 0.06016604908042211 1104 60-61 -0.029563314746098968 1104 62-63 0.010939408841750264 1104 64-65 0.0762666079599228 1104 66-67 0.09166761950571356 1104 68-69 0.018466036372132066 1104 70-71 0.01035592883226144 1104 72-73 0.047621330131548234 1104 74-75 -0.03289175842335368 1104 76-77 -0.020769550524178726 1104 78-79 0.020303533741184765 1104 80-81 -0.08697290784345313 1104 82-83 0.014238549615804175 1104 84-85 0.08970162209336863 1104 86-87 0.018277421876856437 1104 88-89 -6.681470514848797E-4 1104 90-91 0.03549583681289192 1104 92-93 0.0431145737014198 1104 94-95 -0.042799283865839755 1104 96-97 0.005608451587843177 1104 98-99 0.00810041291834196 1104 100-101 -0.0031195037370004286 1104 102-103 2.8445599610904537E-5 1104 104-105 0.10296794890585659 1104 106-107 0.06721189173092057 1104 108-109 0.10131610066332541 1104 110-111 0.0106057420921708 1104 112-113 0.023632249190562504 1104 114-115 -0.04367023916321955 1104 116-117 0.14751171077935155 1104 118-119 0.09425349343736045 1104 120-121 0.010317612946415977 1104 122-123 0.060220777478619425 1104 124-125 0.10009063807793339 1105 1 0.0688267786933423 1105 2 0.11097994118382815 1105 3 0.2174329850538328 1105 4 0.22487591473103663 1105 5 0.28146078431007027 1105 6 0.21008522905542293 1105 7 0.23710294999119697 1105 8 0.17802519597797328 1105 9 0.20384487199120827 1105 10-11 0.31294131734858865 1105 12-13 0.24177232129679282 1105 14-15 0.28162728626497824 1105 16-17 0.34439877904607386 1105 18-19 0.26494978387878376 1105 20-21 0.40905459986392145 1105 22-23 0.3514612815156184 1105 24-25 0.33545892011309775 1105 26-27 0.32334483500196143 1105 28-29 0.3430307037666225 1105 30-31 0.31147327550169734 1105 32-33 0.3560707182500522 1105 34-35 0.43810456820863664 1105 36-37 0.4368393729121749 1105 38-39 0.4040958202813272 1105 40-41 0.4569563864247925 1105 42-43 0.46696080226259795 1105 44-45 0.4894866595394518 1105 46-47 0.5299393688276268 1105 48-49 0.5085435156023763 1105 50-51 0.5538133636441103 1105 52-53 0.5049482355881878 1105 54-55 0.6515947645793787 1105 56-57 0.6017104442985328 1105 58-59 0.6677959170442591 1105 60-61 0.7030930004785603 1105 62-63 0.759952520964795 1105 64-65 0.5930322652148661 1105 66-67 0.6673220343398114 1105 68-69 0.5310587447431843 1105 70-71 0.5591346848674732 1105 72-73 0.574316861640483 1105 74-75 0.6665892081263323 1105 76-77 0.574909014709533 1105 78-79 0.6018540198942546 1105 80-81 0.6448785529030943 1105 82-83 0.5583964211783794 1105 84-85 0.6354858810649411 1105 86-87 0.622873717544838 1105 88-89 0.6221777116410365 1105 90-91 0.5970188368757974 1105 92-93 0.6486991868280363 1105 94-95 0.6775335608682482 1105 96-97 0.7543165862887449 1105 98-99 0.7133230559670487 1105 100-101 0.6428784300409625 1105 102-103 0.5213249236822222 1105 104-105 0.7336784128718179 1105 106-107 0.7141819410419501 1105 108-109 0.6066632126224434 1105 110-111 0.626277483456068 1105 112-113 0.5318089797944765 1105 114-115 0.6196365929028715 1105 116-117 0.5822031784641375 1105 118-119 0.5710599604835949 1105 120-121 0.6716716364563595 1105 122-123 0.6774112110993293 1105 124-125 0.9607659915181763 1106 1 0.03803726619241843 1106 2 0.1257754644525022 1106 3 0.17920929599122104 1106 4 0.17110259073959355 1106 5 0.17311485171916985 1106 6 0.20948415227984896 1106 7 0.16260446190210587 1106 8 0.08509430136923157 1106 9 0.11052401499585329 1106 10-11 0.1967435889025353 1106 12-13 0.24429785784079172 1106 14-15 0.26508108079259785 1106 16-17 0.2277258127506272 1106 18-19 0.18888775865568164 1106 20-21 0.1790362541157151 1106 22-23 0.25980859695186354 1106 24-25 0.24554769878408678 1106 26-27 0.29553120567826596 1106 28-29 0.4371485406277529 1106 30-31 0.30803183439247306 1106 32-33 0.3821244464011002 1106 34-35 0.423439954299063 1106 36-37 0.2142635007977134 1106 38-39 0.23065244318862455 1106 40-41 0.22948524179760454 1106 42-43 0.2255856086642467 1106 44-45 0.24289445141201327 1106 46-47 0.2747813556998153 1106 48-49 0.34690825488993227 1106 50-51 0.2992508959105038 1106 52-53 0.21014128079801253 1106 54-55 0.14786016025752247 1106 56-57 0.26535451834529766 1106 58-59 0.1619792085849312 1106 60-61 0.1422261408281429 1106 62-63 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-0.7004217074450594 2216 34-35 -0.8548292764701699 2216 36-37 -0.8391249607407474 2216 38-39 -0.768466787580607 2216 40-41 -0.7315801428177764 2216 42-43 -0.6257720836434473 2216 44-45 -0.5401478562802993 2216 46-47 -0.5919494135309549 2216 48-49 -0.7570994374177573 2216 50-51 -0.8206644887048782 2216 52-53 -0.7535741038173711 2216 54-55 -0.8856090705117055 2216 56-57 -0.6331685585777791 2216 58-59 -0.44787848372276073 2216 60-61 -0.5450623207103149 2216 62-63 -0.5854963764465211 2216 64-65 -0.531058242934705 2216 66-67 -0.80141468665731 2216 68-69 -0.7278854546815481 2216 70-71 -0.591551029418234 2216 72-73 -0.5137019303058281 2216 74-75 -0.4008201878468185 2216 76-77 -0.6630089143412761 2216 78-79 -0.6306165457816775 2216 80-81 -0.7663709197718802 2216 82-83 -0.7308787466068551 2216 84-85 -0.8539150777078284 2216 86-87 -0.9224486218607204 2216 88-89 -0.8205862385027771 2216 90-91 -0.7232477019545058 2216 92-93 -1.0849840346484783 2216 94-95 -1.011719363388707 2216 96-97 -0.9526460215731909 2216 98-99 -1.0179640006005464 2216 100-101 -0.766864235347338 2216 102-103 -0.9901600235852754 2216 104-105 -0.9872857290265493 2216 106-107 -1.1642107722849886 2216 108-109 -1.0881153108674901 2216 110-111 -0.873209764866079 2216 112-113 -0.8427263989207674 2216 114-115 -0.9527127838948317 2216 116-117 -1.0520342136739238 2216 118-119 -0.8762777192862998 2216 120-121 -1.0425189675704871 2216 122-123 -1.155865107213227 2216 124-125 -1.5811419662958208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 41.0 5 78.0 6 244.0 7 453.0 8 671.0 9 1137.0 10 2724.0 11 8919.0 12 13124.0 13 4071.0 14 3295.0 15 4176.0 16 2469.0 17 1592.0 18 1370.0 19 1300.0 20 1222.0 21 1298.0 22 1426.0 23 1672.0 24 2178.0 25 2800.0 26 3820.0 27 4782.0 28 6166.0 29 8182.0 30 10555.0 31 13788.0 32 18265.0 33 25158.0 34 44300.0 35 71176.0 36 141699.0 37 253908.0 38 54433.0 39 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 65.34914064609016 11.429463241248989 7.355968235399982 15.865427877260865 2 12.550000070150924 29.702461876481063 42.46193260179951 15.285605451568504 3 34.715709960463435 20.275044264673284 21.421576467749222 23.587669307114055 4 14.395188819990953 16.012261147838988 45.52344480938186 24.069105222788203 5 16.52732965541632 33.20538747945732 29.566579326663305 20.70070353846305 6 30.794906403194446 37.62799233378885 16.128507249508235 15.44859401350847 7 41.05965839738032 24.28075167167538 18.74507195990785 15.914517971036451 8 39.67447576877425 24.46384326798156 17.79538076037028 18.066300202873904 9 48.113665507788056 10.42261188229385 16.14138319036756 25.32233941955053 10-11 35.00486841256078 23.66132684585792 24.595164137056337 16.738640604524957 12-13 28.314799693584643 25.703254119911666 27.428874877588182 18.55307130891551 14-15 24.397130582890686 25.80307762241883 26.422783048383884 23.3770087463066 16-17 22.950215921813573 30.179738424542414 25.655832694967994 21.214212958676015 18-19 23.254596931917987 24.42953989993799 28.577230981623497 23.73863218652053 20-21 23.641263935304828 25.337281377410005 26.8309861130987 24.19046857418647 22-23 24.20990013440747 23.020295808675595 26.8826165402563 25.88718751666063 24-25 21.322173865463455 22.601640388462844 35.03910163056746 21.037084115506243 26-27 19.337909921740618 22.17484756396982 33.347854390971406 25.139388123318152 28-29 21.748545789735086 22.91479015317962 27.743006742821546 27.593657314263748 30-31 22.060292553713882 25.798728320131097 26.895734597156395 25.245244528998622 32-33 27.685273262453734 22.913597602552336 25.203224656896168 24.197904478097758 34-35 26.204826883738942 19.15804522713179 30.012851486759885 24.62427640236939 36-37 24.126982790792947 24.37356017049265 30.37735107848667 21.122105960227735 38-39 22.892412291408863 27.524278927770716 26.79247374284119 22.790835037979228 40-41 21.569242295421446 26.764834628303014 28.166993565838617 23.498929510436923 42-43 22.426265296215544 22.459516413705632 25.674703054893804 29.439515235185016 44-45 23.07978303996588 23.818883828732893 25.157276382727378 27.944056748573846 46-47 23.909587826443197 22.66035596934724 25.61865317541157 27.811403028797994 48-49 19.504095359154157 22.979959537458715 27.265916341872 30.25002876151513 50-51 23.288830149924657 23.977913962382743 26.886334492211944 25.846921395480653 52-53 23.84182289079884 22.35267790750858 29.337797681681582 24.467701520010998 54-55 21.673274800142543 23.9027131228271 28.74306566885254 25.680946408177814 56-57 21.538727029370417 25.404765712906762 27.392607308511447 25.663899949211373 58-59 23.15505402955842 22.8706657799702 30.321862399299622 23.65241779117176 60-61 24.7857617873107 27.210918712943243 27.66044014939152 20.34287935035454 62-63 25.593118561980365 24.214670336916598 26.5058406920722 23.686370409030836 64-65 28.394700585612508 20.85441341942146 27.888006869091615 22.862879125874418 66-67 28.279373924950686 24.25472600798588 24.933848515204318 22.532051551859116 68-69 23.051372275021816 27.475454502089068 25.95944205466652 23.513731168222595 70-71 24.112251283044177 26.737335813838637 24.39825298348107 24.752159919636117 72-73 23.049478224025545 27.389941607109286 24.732167159119925 24.828413009745244 74-75 21.65412384006914 26.93333501693422 26.23162419783433 25.180916945162313 76-77 22.8447804163545 27.40018351249653 23.91653268009619 25.838503391052786 78-79 22.99532239553958 27.852721400531454 22.067447857477575 27.08450834645139 80-81 22.502798986472268 23.230886219446152 25.558885343973692 28.70742945010789 82-83 24.50866914155997 20.42537579374767 27.364617443788774 27.701337620903587 84-85 25.36218464050912 21.734796382502896 29.317454170980895 23.585564806007085 86-87 26.123031940714803 23.936034390354088 24.788918538971153 25.15201512995996 88-89 22.646466262041255 25.080812842507555 26.87798663782097 25.39473425763022 90-91 20.90632444672666 26.283885975324022 26.3311671001939 26.478622477755426 92-93 23.87823075994938 23.244986376862833 26.550596415613715 26.326186447574074 94-95 22.62338689990151 22.871998630671282 27.7590009512345 26.745613518192712 96-97 22.44941480839219 21.66064779350074 27.879378414553045 28.010558983554024 98-99 20.87468678008525 23.75224129367892 28.684420238005043 26.688651688230784 100-101 20.656730615440303 26.71053849974325 26.685284486459636 25.947446398356806 102-103 21.931146335782973 25.912792280128738 26.175995218573483 25.980066165514803 104-105 21.679027103168256 23.330709721953312 25.44846918589479 29.54179398898364 106-107 20.820390651525482 25.220341265899503 24.289380126213945 29.669887956361062 108-109 22.804373995100722 24.687341285541518 26.022296487727953 26.485988231629808 110-111 24.026042810272052 26.42821917097101 23.77974309347113 25.765994925285813 112-113 21.38413134902123 26.86727252957703 24.87655338596928 26.872042735432455 114-115 22.262184612211655 24.104616813526697 25.97595101251695 27.657247561744697 116-117 24.385468295643083 23.903510156548702 24.737992262011247 26.97302928579697 118-119 22.2158540058731 25.174221772004135 25.4100016696107 27.199922552512064 120-121 20.43909806504746 25.154593469158122 26.799685446572568 27.606623019221853 122-123 20.783765165088482 26.28023722568851 27.884681489296923 25.05131611992609 124-125 21.433623797781728 26.68373712522999 24.648614738603047 27.234024338385233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 3.0 2 23.0 3 64.0 4 121.0 5 179.0 6 243.5 7 324.0 8 410.5 9 511.0 10 626.5 11 763.5 12 864.5 13 964.5 14 1073.5 15 1140.0 16 1215.0 17 1304.0 18 1383.5 19 1489.5 20 1549.0 21 1596.0 22 1665.5 23 1782.5 24 1987.5 25 2090.5 26 2245.0 27 2459.0 28 2569.5 29 2748.5 30 3043.5 31 3319.0 32 3701.5 33 4225.0 34 4860.0 35 5274.5 36 5726.0 37 6551.0 38 7116.5 39 7477.0 40 7809.0 41 8416.5 42 9145.0 43 10088.0 44 11076.5 45 13133.0 46 21861.0 47 38013.0 48 51552.5 49 60429.5 50 61832.5 51 53428.5 52 42976.0 53 34264.5 54 28776.0 55 25383.5 56 22214.0 57 19381.0 58 17681.5 59 15789.0 60 13937.0 61 11849.5 62 9095.5 63 6846.0 64 5500.0 65 4942.5 66 4364.0 67 3829.0 68 3232.0 69 2579.0 70 2092.5 71 1618.0 72 1221.0 73 948.0 74 723.5 75 544.0 76 350.5 77 258.0 78 224.0 79 166.0 80 160.5 81 148.0 82 116.5 83 54.0 84 10.0 85 3.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21409791261550204 2 0.0012627006641805494 3 0.0 4 0.12851486759882036 5 0.030445116014131024 6 0.0028060014759567764 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.00119255062728163 112-113 7.015003689891941E-5 114-115 6.313503320902747E-4 116-117 0.005682152988812472 118-119 0.00238510125456326 120-121 2.1045011069675823E-4 122-123 0.0026657014021589376 124-125 0.03100631630932238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 712758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.18778846639881 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69425948226191 40.18359954332594 2 10.256052709848433 10.08945102384522 3 2.846140749551834 4.199861074036601 4 1.2794270163284245 2.5172874174943325 5 0.7757285386716841 1.9078185633765732 6 0.5315813404559054 1.5688386316217884 7 0.37596991807517943 1.294519016000785 8 0.2903303634640039 1.1424566802752083 9 0.23615489101368736 1.0454343142038045 >10 1.4739489725905244 13.58358073262769 >50 0.13688600701040923 4.6603006077746345 >100 0.09097213063977773 8.320871228603668 >500 0.00712947732286441 2.546521288019777 >1k 0.004562865486633223 4.519184550850124 >5k 8.555372787437292E-4 2.420275327943882 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6173 0.8660723555540589 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5653 0.7931163171791829 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 5428 0.7615488005746691 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4942 0.6933629647089194 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 4652 0.6526759433075462 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 3617 0.507465366926783 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2848 0.39957461017624496 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 1758 0.24664752973660065 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1706 0.23935192589911303 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 1603 0.2249010182979356 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1571 0.22041141593640476 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1487 0.2086262097373863 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1476 0.2070829089256101 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1178 0.16527348693385413 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1158 0.16246748545789735 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1105 0.1550315815466119 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1048 0.14703447734013508 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1048 0.14703447734013508 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1020 0.1431060752737956 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 979 0.1373537722480842 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 978 0.13721347217428637 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 951 0.13342537018174472 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 945 0.13258356973895769 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 943 0.132302969591362 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 943 0.132302969591362 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 884 0.12402526523728952 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 802 0.11252065918586672 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 760 0.10662805608635749 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 752 0.10550565549597478 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 744 0.10438325490559207 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 737 0.10340115438900721 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.418004427870329E-4 0.0 22-23 0.0 0.0 0.0 0.0013328507010794688 0.0 24-25 0.0 0.0 0.0 0.003086601623552454 0.0 26-27 0.0 0.0 0.0 0.005331402804317875 0.0 28-29 0.0 0.0 0.0 0.013398657047693607 0.0 30-31 0.0 0.0 0.0 0.04258107239764408 0.0 32-33 0.0 0.0 0.0 0.10761015660294238 0.0 34-35 0.0 0.0 0.0 0.17390194147242122 0.0 36-37 0.0 0.0 0.0 0.2606073870794856 0.0 38-39 0.0 0.0 0.0 0.40357316227948337 0.0 40-41 0.0 0.0 0.0 0.6160576240463103 0.0 42-43 0.0 0.0 0.0 0.9963409740753524 0.0 44-45 0.0 0.0 0.0 1.5225364008541469 0.0 46-47 0.0 0.0 0.0 2.0722320899940794 0.0 48-49 0.0 0.0 0.0 2.3370484792875 0.0 50-51 0.0 0.0 0.0 2.664649151605454 0.0 52-53 0.0 0.0 0.0 2.9348670937400914 0.0 54-55 0.0 0.0 0.0 3.212731389896711 0.0 56-57 0.0 0.0 0.0 3.6022745447964106 0.0 58-59 0.0 0.0 0.0 4.055093032978935 0.0 60-61 0.0 0.0 0.0 4.659014700641732 0.0 62-63 0.0 0.0 0.0 5.392082586235441 0.0 64-65 0.0 0.0 0.0 6.086918701719237 0.0 66-67 0.0 0.0 0.0 6.66579680620912 0.0 68-69 0.0 0.0 0.0 7.149130560442675 0.0 70-71 0.0 0.0 0.0 7.680096189730596 0.0 72-73 0.0 0.0 0.0 8.21281556994099 0.0 74-75 0.0 0.0 0.0 8.748551401738037 0.0 76-77 0.0 0.0 0.0 9.346790916412022 0.0 78-79 0.0 0.0 0.0 10.08378720407207 0.0 80-81 0.0 0.0 0.0 11.626035765294812 0.0 82-83 0.0 0.0 0.0 12.795086130215305 0.0 84-85 0.0 0.0 0.0 13.430645464519515 0.0 86-87 0.0 0.0 0.0 13.974167950412342 0.0 88-89 0.0 0.0 0.0 14.588191223388584 0.0 90-91 0.0 0.0 0.0 15.310806753484353 0.0 92-93 0.0 0.0 0.0 16.191540466750286 0.0 94-95 0.0 0.0 0.0 17.294088596690603 0.0 96-97 0.0 0.0 0.0 18.514839538805596 0.0 98-99 0.0 0.0 0.0 19.754811591030894 0.0 100-101 0.0 0.0 0.0 20.90429009565659 0.0 102-103 0.0 0.0 0.0 21.934022487295827 0.0 104-105 0.0 0.0 0.0 22.979608787274223 7.015003689891941E-5 106-107 0.0 0.0 0.0 23.985069266146432 1.4030007379783882E-4 108-109 0.0 0.0 0.0 24.86552237926477 1.4030007379783882E-4 110-111 0.0 0.0 0.0 25.798938770241794 1.4030007379783882E-4 112-113 0.0 0.0 0.0 28.085829973146566 1.4030007379783882E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 980 0.0 84.67751 1 AGTAGTA 855 0.0 82.91708 2 GAGTTAG 730 0.0 80.96394 1 GAGTCCG 385 0.0 77.53336 1 GAGTACG 325 0.0 77.15166 1 GAGTAAC 455 0.0 73.477776 1 AGTACGT 65 8.913048E-11 73.32293 2 GAGTCGC 240 0.0 72.138336 1 AGTTAGT 450 0.0 71.48986 2 TGTACGG 75 3.6379788E-12 71.48986 2 GAGTCAG 2915 0.0 70.86257 1 GAGTAAA 390 0.0 70.4162 1 GAGTTAT 350 0.0 69.9351 1 GAGTCGG 370 0.0 69.38189 1 GAGTAAG 620 0.0 69.329834 1 AGTCGTA 200 0.0 68.511116 2 AGTACGG 175 0.0 68.08558 2 GAGTCGA 205 0.0 66.98126 1 TGGGGTA 3470 0.0 65.15354 8 GAGTCGT 395 0.0 64.99062 1 >>END_MODULE