FastQCFastQC Report
Thu 26 May 2016
SRR935936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410512
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7670.18683984877421367No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7290.17758311571890714No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7130.17368554390614646No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT6830.16637759675722025No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6050.14737693417001207No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4320.10523443894453754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG507.3960255E-659.5642171
GTACTTT150.004236430459.498922-23
TCCTAGG507.4550608E-659.484412
AGTTTCA300.004239011659.4844067
TAACAAC655.432139E-754.982351
GTCCAAG505.3003023E-447.6513751
TATAAGG802.2944078E-644.6133042
GTATATG1256.148184E-1042.8862341
TATATGG1455.820766E-1141.023732
AAGATGT300.001290871639.774967118-119
TAAGGGT758.1920916E-539.6562734
ATACGGG1508.54925E-1139.6562733
TATACGG1202.075285E-839.656272
TGTAAGG1252.9740477E-838.0700232
TAGGGTG801.19797485E-437.1777535
AGTTAAC801.19797485E-437.1777532
GTGTAAG1156.587725E-736.256481
GTATAAG1156.587725E-736.256481
TCTGAGG1156.657265E-736.20792
GTATACG1503.6652636E-935.738531