FastQCFastQC Report
Thu 26 May 2016
SRR935936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410512
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT66481.61944108820205No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT35120.8555170129009627TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT20970.5108255057099427RNA PCR Primer, Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT8860.21582803913162102TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG7870.19171181354016448TruSeq Adapter, Index 10 (96% over 28bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA7860.19146821530186695No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7010.17076236504657596No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6820.16613399851892272No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6790.1654032038040301No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT6750.16442881085083993No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG5890.14347936235725142TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC5680.13836379935300308No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4560.11108079666367852No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT4530.11035000194878591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA701.8189894E-1276.792661
CTAGGGT508.8311936E-871.498294
TCAGATG851.4551915E-1163.0867274
GTCCAAG701.2991222E-859.7276271
TTCAAGG805.657057E-1059.581912
AACTAGT150.004235537359.50207112-113
TGACAGT150.00423758159.49482314-15
CGACTAT150.00423758159.49482344-45
ATGTGTA1103.6379788E-1254.0862058
TCCAAGG803.7973223E-852.134172
GTCGTAG350.007675939751.1951071
GCTATAT350.007750431551.070212
TGTGTAT1207.2759576E-1249.579029
TAGTGTT505.33086E-447.595869
CGTCAGA1152.7102942E-1046.6293222
GTTCAAG909.4163624E-846.4548151
GTAGTGT653.518021E-545.765258
GTCAGAT1204.110916E-1044.686433
GACTGAC558.525648E-443.2689637
AGTCGTT558.525648E-443.2689637
Produced by FastQC (version 0.11.5)