FastQCFastQC Report
Thu 26 May 2016
SRR935935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651805
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC83261.2773759023020688No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG52910.8117458442325541No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG42310.6491205191736792No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA39280.6026342234257178No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT36230.5558410874417963No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT34890.5352827916324667No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT33560.5148779159411173No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC27420.4206779635013539No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC24850.3812489931804758No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT18200.2792246147237287No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG18200.2792246147237287No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG16950.2600470999762199No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC15520.23810802310506976No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC15180.23289173909374736No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG14340.22000444918342144No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA14270.21893050835756095No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC14010.2149415852900791No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT13800.2117197628124976No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG13720.21049240186865703No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA12940.19852563266621154No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA12390.19008752617730762No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA12050.18487124216598522No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT10800.16569372741847638No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG10780.16538688718251623No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG10090.15480089904189137No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC9860.15127223632834974No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG9770.1498914552665291No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT8750.13424260323256187No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG8740.13408918311458182No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA8290.12718527780547864No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCCTGTC8130.1247305559177975No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA7990.12258267426607651No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA7390.11337746718727228No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT6970.10693382223210929No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA6540.10033675715896626No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT6520.10002991692300613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG208.485432E-489.235453
CGAAACA1200.084.2779166
AGTAGTA13550.080.7962652
GAGTAGT16150.078.575411
AGTTGTA2300.077.607952
GAGTCAG37400.077.259111
GTTAGCG701.8189894E-1276.4875263
GAGACGC551.9699655E-975.825441
AGTTCGC551.9917934E-975.726542
AGTCCGT1500.075.3659442
AGTATGG2300.075.021012
GTCCGTG1550.072.9235843
GAGTCGA2700.072.816491
AGTCAGT29450.072.732742
GAGTTAG9200.071.88111
GAGTAGG6150.071.68631
GTGTTCG753.6379788E-1271.492551
TATTCGG753.6379788E-1271.3993152
AGTCGGT1500.071.3993152
CAACGAG250.002057777771.388365
Produced by FastQC (version 0.11.5)