##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935935_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 651805 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.550632474436373 31.0 26.0 34.0 2.0 34.0 2 26.749635243669502 31.0 26.0 34.0 2.0 34.0 3 26.70662237939261 31.0 26.0 34.0 2.0 34.0 4 29.097891240478365 35.0 28.0 37.0 2.0 37.0 5 29.60347496567225 37.0 31.0 37.0 2.0 37.0 6 29.487170242633916 37.0 31.0 37.0 2.0 37.0 7 29.20424666886569 37.0 30.0 37.0 2.0 37.0 8 28.880027001940764 37.0 27.0 37.0 2.0 37.0 9 29.875640720767713 39.0 23.0 39.0 2.0 39.0 10-11 30.132288798030086 39.0 27.0 39.0 2.0 39.0 12-13 30.147897760833377 38.5 26.5 39.0 2.0 39.0 14-15 31.452679865910817 39.5 27.0 41.0 2.0 41.0 16-17 31.88508372902939 40.0 28.5 41.0 2.0 41.0 18-19 32.154035332653166 40.0 29.5 41.0 2.0 41.0 20-21 32.418533150251996 40.0 30.0 41.0 2.0 41.0 22-23 32.36092619725225 40.0 30.0 41.0 2.0 41.0 24-25 32.27022959320655 39.5 30.0 41.0 2.0 41.0 26-27 32.269589831314576 39.5 30.0 41.0 2.0 41.0 28-29 32.05554575371468 39.0 30.0 41.0 2.0 41.0 30-31 31.917907963271222 39.0 30.0 41.0 2.0 41.0 32-33 31.877787835318845 39.0 30.0 41.0 2.0 41.0 34-35 31.547542593260253 39.0 30.0 41.0 2.0 41.0 36-37 31.346506240363297 39.0 30.0 41.0 2.0 41.0 38-39 31.256780785664425 39.0 30.0 41.0 2.0 41.0 40-41 30.88538213115886 39.0 30.0 41.0 2.0 41.0 42-43 30.14705088178213 38.5 28.0 41.0 2.0 41.0 44-45 29.692401868657036 38.0 26.5 41.0 2.0 41.0 46-47 29.458087157969025 38.0 25.5 41.0 2.0 41.0 48-49 29.24735235231396 37.0 24.5 41.0 2.0 41.0 50-51 29.009508211811813 36.5 23.0 41.0 2.0 41.0 52-53 28.72379009059458 36.0 21.0 40.0 2.0 41.0 54-55 28.40515798436649 35.0 19.0 40.0 2.0 41.0 56-57 28.11491396966884 35.0 16.5 39.0 2.0 41.0 58-59 27.751999447687574 35.0 9.5 39.0 2.0 41.0 60-61 27.424288705978014 35.0 4.5 38.0 2.0 40.0 62-63 26.956795360575633 35.0 2.0 37.0 2.0 39.0 64-65 26.49654497894309 35.0 2.0 36.5 2.0 39.0 66-67 26.234851681100942 35.0 2.0 36.0 2.0 38.5 68-69 26.04211228818435 35.0 2.0 35.0 2.0 37.0 70-71 25.91830762267856 35.0 2.0 35.0 2.0 37.0 72-73 25.790982732565723 35.0 2.0 35.0 2.0 36.0 74-75 25.60676122459938 35.0 2.0 35.0 2.0 36.0 76-77 25.484394872699657 35.0 2.0 35.0 2.0 35.5 78-79 25.37198318515507 35.0 2.0 35.0 2.0 35.0 80-81 25.2638687951151 35.0 2.0 35.0 2.0 35.0 82-83 25.144312332676183 35.0 2.0 35.0 2.0 35.0 84-85 24.981953191522003 34.5 2.0 35.0 2.0 35.0 86-87 24.85087641242396 34.0 2.0 35.0 2.0 35.0 88-89 24.592696435283557 34.0 2.0 35.0 2.0 35.0 90-91 24.377832327153058 34.0 2.0 35.0 2.0 35.0 92-93 24.258004311105317 34.0 2.0 35.0 2.0 35.0 94-95 24.120636540069498 34.0 2.0 35.0 2.0 35.0 96-97 24.002356533012176 34.0 2.0 35.0 2.0 35.0 98-99 23.77869224691434 34.0 2.0 35.0 2.0 35.0 100-101 23.525612721596183 34.0 2.0 35.0 2.0 35.0 102-103 22.960031757964423 32.5 2.0 35.0 2.0 35.0 104-105 23.31742238859782 33.0 2.0 35.0 2.0 35.0 106-107 23.253854296913953 33.0 2.0 35.0 2.0 35.0 108-109 23.113599926358344 33.0 2.0 35.0 2.0 35.0 110-111 23.021305451783892 33.0 2.0 35.0 2.0 35.0 112-113 22.829822569633556 33.0 2.0 35.0 2.0 35.0 114-115 22.5251785426623 33.0 2.0 35.0 2.0 35.0 116-117 22.215012925644942 32.5 2.0 35.0 2.0 35.0 118-119 21.78968633256879 32.0 2.0 35.0 2.0 35.0 120-121 21.28391236642861 31.0 2.0 35.0 2.0 35.0 122-123 20.62541097414104 31.0 2.0 35.0 2.0 35.0 124-125 18.11752057747332 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.16628119877719527 1101 2 -0.15570101831624328 1101 3 -0.08171569783806376 1101 4 -0.006243728782479252 1101 5 -0.2508212335237161 1101 6 -0.021415101971108186 1101 7 -0.20223434942048613 1101 8 -0.14332038469531128 1101 9 -0.3399851287972879 1101 10-11 -0.13273317661381867 1101 12-13 -0.28880288685875755 1101 14-15 -0.33477318277765633 1101 16-17 -0.29002352116559393 1101 18-19 -0.25536534260410804 1101 20-21 -0.30428594585912094 1101 22-23 -0.24975759053369018 1101 24-25 -0.2487235758679205 1101 26-27 -0.34704633339303115 1101 28-29 -0.33488467963882584 1101 30-31 -0.5864594887379511 1101 32-33 -0.42364775578780467 1101 34-35 -0.5353290719192394 1101 36-37 -0.49338565935661904 1101 38-39 -0.3708233061077024 1101 40-41 -0.49303174934084737 1101 42-43 -0.5079755242480779 1101 44-45 -0.44210687124867576 1101 46-47 -0.3959841589099504 1101 48-49 -0.32998988511739924 1101 50-51 -0.1890845729846582 1101 52-53 -0.3307465473196949 1101 54-55 -0.49822846249455566 1101 56-57 -0.6027282338017699 1101 58-59 -0.4914419516479285 1101 60-61 -0.3511337419656577 1101 62-63 -0.4379714586764507 1101 64-65 -0.542743582335703 1101 66-67 -0.6212429286646532 1101 68-69 -0.43927926221690683 1101 70-71 -0.6937370798064464 1101 72-73 -0.8059182709707464 1101 74-75 -0.9688338450364746 1101 76-77 -0.7840314908699639 1101 78-79 -0.6449198329718762 1101 80-81 -0.7541515267691423 1101 82-83 -0.806663217376677 1101 84-85 -0.7279629244079544 1101 86-87 -0.7346440521904363 1101 88-89 -0.7480261822747565 1101 90-91 -0.8874422367775558 1101 92-93 -1.1734885960018673 1101 94-95 -1.022436487371138 1101 96-97 -1.1269287517524518 1101 98-99 -1.1321237768748809 1101 100-101 -1.14029439185083 1101 102-103 -1.0217816453501243 1101 104-105 -1.2251028760067584 1101 106-107 -1.1407288421448527 1101 108-109 -0.9261115153332788 1101 110-111 -1.0279456030998766 1101 112-113 -1.1458777806666802 1101 114-115 -1.1970202050599816 1101 116-117 -1.0405302367565206 1101 118-119 -1.0078862775574997 1101 120-121 -1.0854824557757041 1101 122-123 -0.9644154495107138 1101 124-125 -0.30602860346050775 1102 1 0.7074967621633448 1102 2 0.6864985375140549 1102 3 0.8594604360539151 1102 4 0.7702232904725079 1102 5 0.9988009932775768 1102 6 0.7025185473179896 1102 7 0.7581395315695083 1102 8 0.6747188579266634 1102 9 0.6958518105529414 1102 10-11 0.7004198558746779 1102 12-13 0.6526966437866655 1102 14-15 0.6241210865173983 1102 16-17 0.4410305805355321 1102 18-19 0.5028798436495237 1102 20-21 0.5693689526000227 1102 22-23 0.5464280449266212 1102 24-25 0.5585663020998908 1102 26-27 0.4805202348236506 1102 28-29 0.42655440729480176 1102 30-31 0.3411654626094389 1102 32-33 0.41273352039874567 1102 34-35 0.5641025511317928 1102 36-37 0.4949577479286802 1102 38-39 0.6141344519800818 1102 40-41 0.9061743545582281 1102 42-43 0.8830397698426324 1102 44-45 1.0351523269563287 1102 46-47 0.7506891474068773 1102 48-49 0.7586179634069943 1102 50-51 0.9231942007406424 1102 52-53 0.7820960242854973 1102 54-55 0.7026781931823471 1102 56-57 0.6945849974183211 1102 58-59 0.5659423696195596 1102 60-61 0.501325790511391 1102 62-63 0.7209422716621745 1102 64-65 0.5808941446144047 1102 66-67 0.6058474163681993 1102 68-69 0.8681851927593982 1102 70-71 0.7543820675708943 1102 72-73 0.7784453529045159 1102 74-75 0.7683705188163508 1102 76-77 0.8515318491030186 1102 78-79 0.9262503481303064 1102 80-81 0.9214414626760998 1102 82-83 0.9106307817532979 1102 84-85 0.9157540204371593 1102 86-87 0.8160433805544898 1102 88-89 0.7997335081801715 1102 90-91 0.7837319510100649 1102 92-93 0.8442753300919534 1102 94-95 0.8906384719896252 1102 96-97 0.9518369601783156 1102 98-99 1.0964862467073253 1102 100-101 1.09727343560985 1102 102-103 0.9036568005138328 1102 104-105 0.8040629320844559 1102 106-107 0.7827892566256658 1102 108-109 0.8785196406527085 1102 110-111 0.9046877050485769 1102 112-113 0.9933201953847863 1102 114-115 1.0725295579731942 1102 116-117 1.0308030736814686 1102 118-119 0.9779472274909224 1102 120-121 0.9205790869126673 1102 122-123 0.6422911695336353 1102 124-125 0.29057544443655914 1103 1 0.47670078008547634 1103 2 0.5240940386490145 1103 3 0.5304135333370184 1103 4 0.5838619578508819 1103 5 0.5317256329747764 1103 6 0.4228397330687095 1103 7 0.38318468736373745 1103 8 0.3164123484830377 1103 9 0.5611500544643242 1103 10-11 0.22674770581186365 1103 12-13 0.3087800054266161 1103 14-15 0.2198446306137143 1103 16-17 0.26533005915460706 1103 18-19 0.052541781763167705 1103 20-21 0.05600283750801793 1103 22-23 0.11720874143116333 1103 24-25 0.25916788511376154 1103 26-27 0.15702614686286154 1103 28-29 0.07096600707697576 1103 30-31 -0.1744011549924238 1103 32-33 0.034024005601125396 1103 34-35 -0.016384619230905173 1103 36-37 0.151434706342215 1103 38-39 0.1236677162991846 1103 40-41 0.25845627229110946 1103 42-43 0.5052043903452486 1103 44-45 0.6200479159765955 1103 46-47 0.7139707470272967 1103 48-49 0.4983332257958608 1103 50-51 0.4602987842559507 1103 52-53 0.42356912760734744 1103 54-55 0.49208909203875706 1103 56-57 0.544921846163934 1103 58-59 0.4852752500470139 1103 60-61 0.5564151855046227 1103 62-63 0.3280113775504887 1103 64-65 0.4125580614953961 1103 66-67 0.5649328969606451 1103 68-69 0.547779895917337 1103 70-71 0.5485013004346087 1103 72-73 0.5787765403266754 1103 74-75 0.6235100569601961 1103 76-77 0.6403988565274403 1103 78-79 0.5996371405322698 1103 80-81 0.5758203615756514 1103 82-83 0.5660758965703323 1103 84-85 0.7567961892092967 1103 86-87 0.6709510740234776 1103 88-89 0.5299721194844871 1103 90-91 0.3490851906350194 1103 92-93 0.27440179327448533 1103 94-95 0.40311090673613137 1103 96-97 0.5212077175555585 1103 98-99 0.33177624126167515 1103 100-101 0.32421567393539164 1103 102-103 0.19871554348435794 1103 104-105 0.21061335290949756 1103 106-107 0.1014435869989363 1103 108-109 0.20777951696626573 1103 110-111 0.1783803437105611 1103 112-113 -0.05510433917354973 1103 114-115 -0.12150825966748968 1103 116-117 -0.09286449340521585 1103 118-119 -0.42574624689024176 1103 120-121 -0.30662060683669523 1103 122-123 -0.3068088148303296 1103 124-125 -0.6488382850815846 1104 1 0.5836354167828794 1104 2 0.5664001619770538 1104 3 0.5401304874582991 1104 4 0.5580429845794725 1104 5 0.6081487803557586 1104 6 0.5877163011469335 1104 7 0.5450435520890409 1104 8 0.6766795077118637 1104 9 0.6864100811701093 1104 10-11 0.7666043205618678 1104 12-13 0.6285685128163117 1104 14-15 0.6200203465898042 1104 16-17 0.7114155328782843 1104 18-19 0.5156623302706578 1104 20-21 0.6318590569920914 1104 22-23 0.6051037223139701 1104 24-25 0.5894366406088452 1104 26-27 0.6582176456048501 1104 28-29 0.6480510982035099 1104 30-31 0.579540102409311 1104 32-33 0.61340721338685 1104 34-35 0.6266573068290278 1104 36-37 0.4551338633616062 1104 38-39 0.4529033328000196 1104 40-41 0.7132667346762354 1104 42-43 0.5228000586360579 1104 44-45 0.3943681725954029 1104 46-47 0.4567064686790019 1104 48-49 0.47025730939967403 1104 50-51 0.5448679570606387 1104 52-53 0.5106885257896288 1104 54-55 0.4603994061991621 1104 56-57 0.5812407041494438 1104 58-59 0.563907832628459 1104 60-61 0.6065715182475948 1104 62-63 0.5780101106725084 1104 64-65 0.6835590978015844 1104 66-67 0.5680316805044043 1104 68-69 0.5782597132842078 1104 70-71 0.5339132004728668 1104 72-73 0.6009722425698101 1104 74-75 0.7142837646813085 1104 76-77 0.6339168753329787 1104 78-79 0.6616457502350386 1104 80-81 0.6870395796078839 1104 82-83 0.7840751921861724 1104 84-85 0.8111376533655204 1104 86-87 0.8410528803381041 1104 88-89 0.8645602954589116 1104 90-91 0.7031737859460492 1104 92-93 0.6315289954251355 1104 94-95 0.7390799797108372 1104 96-97 0.7349503031521678 1104 98-99 0.7349614258837995 1104 100-101 0.673284898682077 1104 102-103 0.7031559508109666 1104 104-105 0.793557754702384 1104 106-107 0.6419444092027469 1104 108-109 0.652021049787276 1104 110-111 0.6668606222210194 1104 112-113 0.735651758943618 1104 114-115 0.8082693272340045 1104 116-117 0.7045541160188371 1104 118-119 0.6500371271098757 1104 120-121 0.6188685980960216 1104 122-123 0.5806309767935822 1104 124-125 0.8924936090783611 1105 1 0.40058749760772727 1105 2 0.40284180308634276 1105 3 0.6190225480624605 1105 4 0.3416791858887507 1105 5 0.6001883916745214 1105 6 0.6660171840527269 1105 7 0.6053074827692342 1105 8 0.5482009095510278 1105 9 0.8290564338450075 1105 10-11 0.8646740650524549 1105 12-13 0.7985435627044701 1105 14-15 0.8710530978342916 1105 16-17 0.7914308311093379 1105 18-19 0.926284104431133 1105 20-21 0.9289988427685998 1105 22-23 0.9479783419695451 1105 24-25 1.0418034024269716 1105 26-27 0.8921050371116763 1105 28-29 0.8234770477676037 1105 30-31 0.8754492733851365 1105 32-33 0.7821045955554808 1105 34-35 0.8298474947270158 1105 36-37 0.80549532629718 1105 38-39 0.9230494622751735 1105 40-41 1.0080412064167312 1105 42-43 0.8731259302612173 1105 44-45 0.9019913486915527 1105 46-47 0.877395154503045 1105 48-49 0.7481572773071683 1105 50-51 0.7003054647519811 1105 52-53 0.7866164938032973 1105 54-55 0.8173657718075802 1105 56-57 0.8334564140332148 1105 58-59 0.6362378207807744 1105 60-61 0.7679551877478907 1105 62-63 0.6657800918754688 1105 64-65 0.5726933723541592 1105 66-67 0.6123434652095519 1105 68-69 0.7574975525176555 1105 70-71 0.7591470776092493 1105 72-73 0.7750185764235411 1105 74-75 0.6750953189478004 1105 76-77 0.7113279754784827 1105 78-79 0.6477687586297627 1105 80-81 0.7038350586669218 1105 82-83 0.8116759517891907 1105 84-85 0.7455422650341603 1105 86-87 0.7320825429807414 1105 88-89 0.8713787459942246 1105 90-91 1.012696331533089 1105 92-93 0.9392424166066675 1105 94-95 0.8772689731885812 1105 96-97 0.9773802124003623 1105 98-99 0.9944980873464964 1105 100-101 1.154341876147349 1105 102-103 1.1086459406041023 1105 104-105 1.0504262743693005 1105 106-107 1.04600471415392 1105 108-109 1.027695776578728 1105 110-111 0.9724632977223742 1105 112-113 0.6754855535564204 1105 114-115 0.8012192315597382 1105 116-117 0.7015475551528709 1105 118-119 0.4716052199223597 1105 120-121 0.5693803890748939 1105 122-123 0.639135248957313 1105 124-125 0.5076369274432366 1106 1 0.3104524773954722 1106 2 0.2110513581836777 1106 3 0.550674827329459 1106 4 -0.08537728028629843 1106 5 0.35317596142719765 1106 6 0.33127307007190154 1106 7 0.5463319588232416 1106 8 0.5526045023235433 1106 9 0.7344187982714345 1106 10-11 0.6097048785521473 1106 12-13 0.770391440708643 1106 14-15 0.651267802106247 1106 16-17 0.5275158274396716 1106 18-19 0.47936661937188774 1106 20-21 0.49182984033380706 1106 22-23 0.43424973376115616 1106 24-25 0.35275297080497836 1106 26-27 0.4826949787465935 1106 28-29 0.2968201668626591 1106 30-31 0.32848518946058647 1106 32-33 0.4479511221925563 1106 34-35 0.6322024848830061 1106 36-37 0.6373607953730023 1106 38-39 0.738117787861448 1106 40-41 0.911931629184938 1106 42-43 0.8868312390369475 1106 44-45 0.9850631458932888 1106 46-47 1.047887588012287 1106 48-49 1.0159155223939003 1106 50-51 0.984158375433605 1106 52-53 0.9714974919509949 1106 54-55 1.016264291128632 1106 56-57 1.0387436377893167 1106 58-59 1.1489546511259583 1106 60-61 1.0767920800695627 1106 62-63 0.9744993220622149 1106 64-65 0.8050201701966344 1106 66-67 0.7634851374098339 1106 68-69 0.7556132109013731 1106 70-71 0.7292253637029837 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-2.5139132754783233 2216 102-103 -2.5018301936832152 2216 104-105 -2.790876301175363 2216 106-107 -2.6215187689636217 2216 108-109 -2.7255267829469965 2216 110-111 -2.738407410150149 2216 112-113 -3.0397769531926286 2216 114-115 -2.881426145654043 2216 116-117 -2.9019761639579364 2216 118-119 -2.9158537047349995 2216 120-121 -2.7642921845746713 2216 122-123 -2.47889624541639 2216 124-125 -2.174995934976266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82990.0 3 5409.0 4 1870.0 5 3674.0 6 7230.0 7 11762.0 8 22720.0 9 10494.0 10 6297.0 11 4433.0 12 3191.0 13 2416.0 14 2201.0 15 2270.0 16 2154.0 17 2035.0 18 2077.0 19 2025.0 20 2103.0 21 2292.0 22 2875.0 23 3622.0 24 4763.0 25 6260.0 26 7125.0 27 6521.0 28 7433.0 29 9022.0 30 11188.0 31 14322.0 32 19597.0 33 26144.0 34 38705.0 35 57138.0 36 121936.0 37 120953.0 38 14512.0 39 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 77.65772078143583 8.789770160509649 5.220481805526105 8.33202725252842 2 10.784924490491111 40.25480814968713 39.55648712763117 9.40378023219059 3 44.495843574801846 21.86626406571724 15.383376140370258 18.254516219110656 4 13.827887164882647 24.021039968575074 43.157713836786805 18.993359029755474 5 17.08911933156282 34.01775728034686 24.05763214782698 24.835491240263334 6 28.39791041799311 42.67119767415101 11.471529061605848 17.45936284625003 7 44.61240589564638 28.61907662284463 11.671317867515185 15.097199613993798 8 42.557118397555094 27.49216021994342 12.386852489398652 17.563868893102836 9 56.37529629260285 18.851803837037153 7.184357284770752 17.58854258558925 10-11 39.81666295901382 31.639830931029987 11.659698836308404 16.883807273647793 12-13 27.906429070043952 33.110976442340885 17.996256549121288 20.986337938493875 14-15 25.46635880362992 29.744018533150253 17.87436426538612 26.915258397833707 16-17 25.75064628224699 33.97565222727656 19.13279278311765 21.140908707358797 18-19 24.979940319574105 27.245341781667832 23.136214051748606 24.638503847009456 20-21 26.587706445946257 26.347143700953506 19.512507575118327 27.55264227798191 22-23 33.55566465430611 20.202821395969654 21.008277015365024 25.233236934359205 24-25 30.092972591495926 23.481179187026797 26.795974256104206 19.62987396537308 26-27 24.53910077762001 26.987180814870896 24.062878521133513 24.41083988637558 28-29 23.10444637597995 29.227985034265153 21.453213666781483 26.214354922973413 30-31 21.027684660289502 32.27828875200405 22.550762881536656 24.14326370616979 32-33 28.277552335437743 33.34847078497403 18.14108514049447 20.23289173909375 34-35 26.342924647709054 28.886630203818626 19.968625585873077 24.801819562599245 36-37 21.570254907526024 37.60219697608947 20.725447027868764 20.102101088515738 38-39 18.622517470715934 43.11949125888878 18.09022637138408 20.167764899011207 40-41 19.15158674757021 44.40499842744379 16.687506232692293 19.755908592293707 42-43 18.543659530074176 39.539202675646855 14.908293124477412 27.00884466980155 44-45 19.32188307852809 37.85825515299821 17.259226302345027 25.560635466128673 46-47 22.32285729627726 36.14923174875921 18.93863962381387 22.589271331149654 48-49 16.350442233490078 36.46520048173917 21.771388682197895 25.412968602572857 50-51 20.137617845828125 38.281771388682195 20.669755525042 20.910855240447678 52-53 22.64948872745683 35.58242112288184 21.640751451737867 20.12733869792346 54-55 16.80563972353695 35.9930500686555 23.655234310875183 23.546075896932365 56-57 16.135995599880637 37.513395586532994 20.524761794444775 25.825847019141595 58-59 16.66341927416942 36.55295678922377 25.541611371499144 21.24201256510766 60-61 18.98021647578647 37.55670791110838 24.037787375058493 19.425288238046654 62-63 21.69191706108422 33.93047000253143 23.408688181281214 20.968924755103135 64-65 25.769333443797006 29.904893847480352 25.500154955983295 18.825617752739348 66-67 26.451873222722455 30.180752050653226 23.11536664751471 20.25200807910961 68-69 21.931131408195924 32.25290481355953 23.17207328430733 22.643890493937214 70-71 21.037468399105393 33.45962182504517 21.110433378217152 24.392476397632283 72-73 20.88141459594096 30.719794048675634 22.601715698495482 25.79707565688792 74-75 22.02154045144576 28.773203856383695 26.403738854055348 22.801516838115198 76-77 22.20792002497685 26.95405900704737 26.653278173559976 24.1847427944158 78-79 21.485288037195488 28.704762737621003 27.01237113560228 22.79757808958123 80-81 19.360475730940767 24.838024722421245 30.689079575389272 25.112419971248716 82-83 20.04794378686877 24.6790067581562 28.52379162479576 26.749257830179275 84-85 19.95737165076671 27.29932988480525 27.677276696042068 25.066021768385973 86-87 21.468790465611516 28.519089028948063 22.970812595506516 27.041307909933902 88-89 19.80808094758018 31.006504716264555 23.246803267242715 25.938611068912547 90-91 19.185646013761783 33.809114689209196 22.18569971080308 24.819539586225943 92-93 19.353638392566015 33.88986964283933 21.982033405988403 24.77445855860625 94-95 18.032897563370774 32.983413122199714 23.1472104584791 25.836478855950407 96-97 20.004955747324395 32.81433515120783 22.85573788852944 24.324971212938337 98-99 20.398165664041585 33.320215591241954 24.071670433223023 22.209948311493445 100-101 18.126251820917595 36.335657919869654 23.650334726653323 21.887755532559424 102-103 17.44148940250535 38.906114558802095 23.01294098695162 20.639455051740935 104-105 16.604761269160374 40.1471461381618 22.265384680293526 20.982707912384303 106-107 16.17142120718382 41.48524542879849 20.55667553444472 21.786657829572967 108-109 17.134495746427227 41.76724633901244 20.03781805908209 21.06043985547825 110-111 17.426530941002294 41.586747570208885 20.228979526085254 20.75774196270357 112-113 18.5377842999025 40.6499094428723 20.541240889630767 20.27106536759443 114-115 20.53796914843671 39.51241703310697 20.220140357122034 19.729473461334283 116-117 22.246454077523186 39.74777732604076 18.223011483495828 19.782757112940217 118-119 21.3408862525151 39.14723356038664 18.760186115638273 20.75169407145999 120-121 20.836874840823942 38.85859642770569 18.609407266713102 21.695121464757268 122-123 22.087171587610392 38.86489447230013 18.09996440473049 20.94796953535899 124-125 22.192317008931834 41.77267851169443 16.55914888054106 19.475855598832673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 78.0 1 117.5 2 177.5 3 198.5 4 180.0 5 169.0 6 169.0 7 155.5 8 154.5 9 156.0 10 172.0 11 202.5 12 221.0 13 265.0 14 333.5 15 430.5 16 557.0 17 717.5 18 899.0 19 1102.0 20 1422.5 21 1871.0 22 2419.0 23 3096.5 24 4011.0 25 5199.5 26 6481.5 27 7880.0 28 9216.5 29 10229.0 30 10856.0 31 11174.5 32 11392.0 33 11391.0 34 11113.0 35 10519.5 36 9886.0 37 9360.0 38 8565.0 39 7741.5 40 7024.5 41 6353.5 42 5777.5 43 5288.0 44 5069.5 45 5445.5 46 7431.5 47 12900.0 48 22716.5 49 39991.5 50 59833.0 51 66552.5 52 58350.5 53 43739.0 54 30714.5 55 24067.5 56 20171.0 57 16106.0 58 12462.5 59 9257.0 60 6835.0 61 5207.0 62 3926.5 63 2810.5 64 1954.0 65 1606.5 66 1508.0 67 1434.0 68 1234.5 69 917.5 70 756.5 71 694.5 72 676.5 73 702.0 74 580.5 75 416.5 76 278.0 77 193.0 78 156.0 79 117.0 80 87.5 81 73.5 82 64.0 83 31.0 84 14.0 85 11.5 86 6.5 87 4.0 88 4.0 89 3.0 90 2.5 91 1.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15388037833401094 2 0.015188591680027001 3 0.006597065073143042 4 0.014268070972146578 5 0.0024547218876811317 6 0.0 7 3.0684023596014146E-4 8 1.5342011798007073E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0013040710028306012 28-29 0.007594295840013501 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.903905309103182E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.001073940825860495 66-67 7.671005899003537E-5 68-69 0.0064436449551629705 70-71 0.018793964452558664 72-73 7.671005899003537E-5 74-75 0.008361396429913855 76-77 2.301301769701061E-4 78-79 8.43810648890389E-4 80-81 0.0012273609438405659 82-83 0.0 84-85 0.019330934865488914 86-87 0.0026081420056612023 88-89 0.01902409462952877 90-91 0.0 92-93 0.014651621267096755 94-95 0.013040710028306013 96-97 0.005599834306272582 98-99 0.0024547218876811317 100-101 6.903905309103182E-4 102-103 0.0 104-105 6.136804719202829E-4 106-107 0.001073940825860495 108-109 0.0 110-111 0.0 112-113 9.972307668704597E-4 114-115 0.005983384601222758 116-117 0.0 118-119 0.07509914775124463 120-121 0.001687621297780778 122-123 0.005062863893342334 124-125 0.24585573906306335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 651805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.083567813238844 #Duplication Level Percentage of deduplicated Percentage of total 1 82.96294207885333 38.23228367276643 2 8.347807781594058 7.693935319899453 3 2.784322326962133 3.849345203054232 4 1.441836099907132 2.657798067425845 5 0.8664100090593964 1.9963632203278787 6 0.5948220744228233 1.6446914042085345 7 0.41791892742992565 1.3481436662857116 8 0.31119123454966113 1.1472641888203858 9 0.25637077862134794 1.0633032145736756 >10 1.7510591946110783 15.595788792594542 >50 0.15465860044299365 4.948963440943383 >100 0.09366178038091581 8.016740063362466 >500 0.008666214554817827 2.620635784350309 >1k 0.007666266721569618 7.093119937267955 >5k 6.666318888321407E-4 2.0916240241192288 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 8326 1.2773759023020688 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 5291 0.8117458442325541 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4231 0.6491205191736792 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3928 0.6026342234257178 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3623 0.5558410874417963 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3489 0.5352827916324667 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3356 0.5148779159411173 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 2742 0.4206779635013539 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2485 0.3812489931804758 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1820 0.2792246147237287 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1820 0.2792246147237287 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1695 0.2600470999762199 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1552 0.23810802310506976 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1518 0.23289173909374736 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1434 0.22000444918342144 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1427 0.21893050835756095 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1401 0.2149415852900791 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1380 0.2117197628124976 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1372 0.21049240186865703 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1294 0.19852563266621154 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1239 0.19008752617730762 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1205 0.18487124216598522 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1080 0.16569372741847638 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1078 0.16538688718251623 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1009 0.15480089904189137 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 986 0.15127223632834974 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 977 0.1498914552665291 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 875 0.13424260323256187 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 874 0.13408918311458182 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 829 0.12718527780547864 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCCTGTC 813 0.1247305559177975 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 799 0.12258267426607651 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 739 0.11337746718727228 No Hit GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT 697 0.10693382223210929 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 654 0.10033675715896626 No Hit CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT 652 0.10002991692300613 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5342011798007073E-4 0.0 12-13 0.0 0.0 0.0 3.0684023596014146E-4 0.0 14-15 1.5342011798007073E-4 0.0 0.0 4.602603539402122E-4 0.0 16-17 1.5342011798007073E-4 0.0 0.0 4.602603539402122E-4 0.0 18-19 1.5342011798007073E-4 0.0 0.0 4.602603539402122E-4 0.0 20-21 1.5342011798007073E-4 0.0 0.0 0.0017643313567708135 0.0 22-23 1.5342011798007073E-4 0.0 0.0 0.008591526606883962 0.0 24-25 1.5342011798007073E-4 0.0 0.0 0.016185822446897462 0.0 26-27 1.5342011798007073E-4 0.0 0.0 0.029379952593183545 0.0 28-29 1.5342011798007073E-4 0.0 0.0 0.05523124247282546 0.0 30-31 1.5342011798007073E-4 0.0 0.0 0.1486640943226885 0.0 32-33 1.5342011798007073E-4 0.0 0.0 0.3374475494971656 0.0 34-35 1.5342011798007073E-4 0.0 0.0 0.5714899394757635 0.0 36-37 1.5342011798007073E-4 0.0 0.0 0.8978145304193739 0.0 38-39 1.5342011798007073E-4 0.0 0.0 1.3606830263652472 0.0 40-41 1.5342011798007073E-4 0.0 0.0 1.9675363030354172 0.0 42-43 1.5342011798007073E-4 0.0 0.0 3.013171117128589 0.0 44-45 1.5342011798007073E-4 0.0 0.0 4.435835871157785 0.0 46-47 1.5342011798007073E-4 0.0 0.0 5.90000076710059 0.0 48-49 1.5342011798007073E-4 0.0 0.0 6.455688434424406 0.0 50-51 1.5342011798007073E-4 0.0 0.0 7.199392456332799 0.0 52-53 1.5342011798007073E-4 0.0 0.0 7.753008952063884 0.0 54-55 1.5342011798007073E-4 0.0 0.0 8.299031151954956 0.0 56-57 1.5342011798007073E-4 0.0 0.0 9.332622486786693 0.0 58-59 1.5342011798007073E-4 0.0 0.0 10.405029111467385 0.0 60-61 1.5342011798007073E-4 0.0 0.0 11.838893534109129 0.0 62-63 1.5342011798007073E-4 0.0 0.0 13.499973151479352 0.0 64-65 1.5342011798007073E-4 0.0 0.0 14.782105077438803 0.0 66-67 1.5342011798007073E-4 0.0 0.0 15.78877118156504 0.0 68-69 1.5342011798007073E-4 0.0 0.0 16.66242204340255 0.0 70-71 1.5342011798007073E-4 0.0 0.0 17.694862727349438 0.0 72-73 1.5342011798007073E-4 0.0 0.0 18.710657328495486 0.0 74-75 1.5342011798007073E-4 0.0 0.0 19.780839361465468 0.0 76-77 1.5342011798007073E-4 0.0 0.0 21.0415691809667 0.0 78-79 1.5342011798007073E-4 0.0 0.0 22.597325887343608 0.0 80-81 1.5342011798007073E-4 0.0 0.0 24.279347350818114 0.0 82-83 1.5342011798007073E-4 0.0 0.0 25.51591350173748 0.0 84-85 1.5342011798007073E-4 0.0 0.0 26.435667109028007 0.0 86-87 1.5342011798007073E-4 0.0 0.0 27.323202491542716 0.0 88-89 1.5342011798007073E-4 0.0 0.0 28.71794478409954 0.0 90-91 1.5342011798007073E-4 0.0 0.0 30.769248471552075 0.0 92-93 1.5342011798007073E-4 0.0 0.0 33.62401331686624 0.0 94-95 1.5342011798007073E-4 0.0 0.0 37.3779734736616 0.0 96-97 1.5342011798007073E-4 0.0 0.0 41.25367249407415 0.0 98-99 1.5342011798007073E-4 0.0 0.0 44.03149715022131 0.0 100-101 1.5342011798007073E-4 0.0 0.0 45.68674680310829 0.0 102-103 1.5342011798007073E-4 0.0 0.0 47.00784743903468 0.0 104-105 1.5342011798007073E-4 1.5342011798007073E-4 0.0 48.33915051280674 0.0 106-107 1.5342011798007073E-4 1.5342011798007073E-4 0.0 49.63286565767369 0.0 108-109 1.5342011798007073E-4 1.5342011798007073E-4 0.0 50.76840466090319 0.0 110-111 1.5342011798007073E-4 1.5342011798007073E-4 0.0 51.67143547533388 0.0 112-113 1.5342011798007073E-4 1.5342011798007073E-4 0.0 52.77406586325665 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGCG 20 8.485432E-4 89.23545 3 CGAAACA 120 0.0 84.277916 6 AGTAGTA 1355 0.0 80.796265 2 GAGTAGT 1615 0.0 78.57541 1 AGTTGTA 230 0.0 77.60795 2 GAGTCAG 3740 0.0 77.25911 1 GTTAGCG 70 1.8189894E-12 76.487526 3 GAGACGC 55 1.9699655E-9 75.82544 1 AGTTCGC 55 1.9917934E-9 75.72654 2 AGTCCGT 150 0.0 75.365944 2 AGTATGG 230 0.0 75.02101 2 GTCCGTG 155 0.0 72.923584 3 GAGTCGA 270 0.0 72.81649 1 AGTCAGT 2945 0.0 72.73274 2 GAGTTAG 920 0.0 71.8811 1 GAGTAGG 615 0.0 71.6863 1 GTGTTCG 75 3.6379788E-12 71.49255 1 TATTCGG 75 3.6379788E-12 71.399315 2 AGTCGGT 150 0.0 71.399315 2 CAACGAG 25 0.0020577777 71.38836 5 >>END_MODULE