FastQCFastQC Report
Thu 26 May 2016
SRR935935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651805
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC69841.071486103972814No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG56520.8671305068233598No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT39050.5991055607121762No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG36400.5584492294474575No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT36020.5526192649642148No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA34550.5300665076211444No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT33270.5104287325196953No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC22650.34749656722486016No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT20940.3212617270502681No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC19730.3026978927746795No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG19010.2916516442801144No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG17980.2758493721281672No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG15640.23994906452083062No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC14730.22598783378464415No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA14680.22522073319474384No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC14100.21632236635189972No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT13950.21402106458219866No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG13360.20496927762137448No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC12170.18671228358174607No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG11710.1796549581546628No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA11460.17581945520516107No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA11140.17091001142979878No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA10560.1620116445869547No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT10500.16109112387907426No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG9750.14958461503056897No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC9640.1478969937327882No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG9560.14666963278894762No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT9460.1451354316091469No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG9140.14022598783378465No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCCTGTC8200.12580449674365798No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA7990.12258267426607651No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT7490.11491166836707298No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT7400.11353088730525233No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT7070.10846802341190999No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT6740.10340515951856766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT454.0017767E-1092.697193
AGTACGT1350.092.697182
GAGTCTA2350.088.957681
GTACGTT700.085.130073
GAGTCGA3450.084.832111
GAGTTAG8850.084.362611
GAGTTAT4250.084.32291
AGTTAGG2700.083.868882
AGTTTCG1000.083.427472
AGTCGAT1150.082.909292
AGTCGTG1400.080.8735662
AGTTCGT900.079.4547352
GTACGGT454.2544343E-879.4547353
GTCGATT750.079.454733
GAGTATG6400.078.393961
AGTAGTA11450.078.066882
AGTTATT1150.077.7274552
GAGTTGT5550.077.485911
GTTAACG850.077.2961
GAGTGAT3800.077.018621
Produced by FastQC (version 0.11.5)