##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935933_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1056326 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.031762921673803 31.0 31.0 34.0 28.0 34.0 2 30.994070959154655 33.0 31.0 34.0 27.0 34.0 3 31.18205364631752 34.0 31.0 34.0 27.0 34.0 4 34.40934427440014 37.0 35.0 37.0 31.0 37.0 5 34.52060538129327 37.0 35.0 37.0 31.0 37.0 6 34.625172531964566 37.0 35.0 37.0 31.0 37.0 7 34.610969530239714 37.0 35.0 37.0 32.0 37.0 8 34.528992943466314 37.0 35.0 37.0 31.0 37.0 9 36.12113211262432 39.0 37.0 39.0 31.0 39.0 10-11 35.91275183986761 39.0 37.0 39.0 30.5 39.0 12-13 35.746210923521716 39.0 37.0 39.0 30.0 39.0 14-15 37.21243489225864 40.0 38.0 41.0 30.5 41.0 16-17 37.374118406628256 40.0 38.0 41.0 31.0 41.0 18-19 37.4456668679934 40.0 38.0 41.0 31.0 41.0 20-21 37.576822874756466 40.0 38.0 41.0 31.0 41.0 22-23 37.5052554798424 40.0 38.0 41.0 31.0 41.0 24-25 37.47628904334458 40.0 38.0 41.0 31.0 41.0 26-27 37.45192582592874 40.0 37.5 41.0 30.5 41.0 28-29 37.35694094436755 40.0 37.0 41.0 30.0 41.0 30-31 37.276796178452486 40.0 37.0 41.0 30.0 41.0 32-33 37.19333094139499 40.0 37.0 41.0 30.0 41.0 34-35 36.97103119680856 40.0 36.0 41.0 30.0 41.0 36-37 36.97041538312983 40.0 36.0 41.0 30.0 41.0 38-39 37.080001344281975 40.0 36.0 41.0 30.0 41.0 40-41 37.04952022387028 40.0 36.0 41.0 30.0 41.0 42-43 36.896834878626485 40.0 35.5 41.0 30.0 41.0 44-45 36.708761783767514 40.0 35.0 41.0 30.0 41.0 46-47 36.5429403422807 40.0 35.0 41.0 30.0 41.0 48-49 36.3728233518819 39.5 35.0 41.0 30.0 41.0 50-51 36.135606337437494 39.0 35.0 41.0 30.0 41.0 52-53 35.87952204149098 39.0 35.0 41.0 30.0 41.0 54-55 35.605013982425874 38.5 35.0 41.0 29.5 41.0 56-57 35.30073765106606 37.5 35.0 40.0 29.0 41.0 58-59 34.912537417426066 37.0 34.0 40.0 28.0 41.0 60-61 34.58845328052135 37.0 34.0 39.5 28.0 41.0 62-63 34.160498274207015 36.0 34.0 39.0 27.0 41.0 64-65 33.73561902291527 35.5 34.0 38.5 26.5 41.0 66-67 33.43642729611881 35.0 33.5 37.0 26.5 40.0 68-69 33.23858969674134 35.0 33.5 37.0 27.0 39.5 70-71 33.06531459038213 35.0 34.0 36.5 27.0 39.0 72-73 32.81431631901515 35.0 34.0 36.0 27.0 39.0 74-75 32.525473196721464 35.0 33.0 35.5 26.5 37.5 76-77 32.33952965277764 35.0 33.0 35.0 27.0 37.0 78-79 32.15387863216469 35.0 33.0 35.0 26.0 37.0 80-81 31.978149737865017 35.0 33.0 35.0 26.0 36.0 82-83 31.86117117253575 35.0 33.0 35.0 26.0 36.0 84-85 31.645574377606913 35.0 33.0 35.0 25.0 35.5 86-87 31.472296431215362 35.0 33.0 35.0 25.0 35.0 88-89 31.363746608528047 35.0 33.0 35.0 25.0 35.0 90-91 31.304596781675354 35.0 33.0 35.0 24.0 35.0 92-93 31.20931748342841 35.0 33.0 35.0 23.5 36.0 94-95 31.06152172719407 35.0 33.0 35.0 23.0 35.0 96-97 30.956753407565465 35.0 32.5 35.0 22.0 35.0 98-99 30.809648252528103 35.0 32.0 35.0 20.0 35.0 100-101 30.69144373990605 35.0 32.0 35.0 19.5 35.0 102-103 29.99797363692648 34.0 30.5 35.0 18.5 35.0 104-105 30.52956331662763 35.0 32.0 35.0 19.0 35.0 106-107 30.564813798013112 35.0 32.0 35.0 18.5 35.0 108-109 30.469166715578332 35.0 32.0 35.0 17.5 35.0 110-111 30.362136783530843 35.0 32.0 35.0 16.0 35.0 112-113 30.28246724969375 35.0 32.0 35.0 10.5 35.0 114-115 30.127189428263623 35.0 32.0 35.0 4.5 35.0 116-117 29.950861760479246 35.0 31.0 35.0 2.0 35.0 118-119 29.720317875352873 35.0 31.0 35.0 2.0 35.0 120-121 29.490040006588874 35.0 31.0 35.0 2.0 35.0 122-123 29.084138798060447 35.0 31.0 35.0 2.0 35.0 124-125 26.35463767814103 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11765779716528613 1101 2 0.2557124600821403 1101 3 0.14001946384490083 1101 4 0.16333151003864543 1101 5 0.19577578222972392 1101 6 0.10443572122589728 1101 7 0.034339251439966745 1101 8 -0.08431437011073228 1101 9 -0.016124967820395852 1101 10-11 0.18206396933527458 1101 12-13 0.021457701611396374 1101 14-15 0.03228803086729215 1101 16-17 0.14626662349105146 1101 18-19 0.29191379849249444 1101 20-21 0.1674466605008007 1101 22-23 0.16451670224892467 1101 24-25 0.24605862387925015 1101 26-27 0.252433738388369 1101 28-29 0.18017723569072785 1101 30-31 -0.00929084486808307 1101 32-33 0.029419944469744053 1101 34-35 -0.12962964437846125 1101 36-37 -0.0454707494644353 1101 38-39 0.031397698088333925 1101 40-41 -0.026433933570423562 1101 42-43 0.06965162855518514 1101 44-45 -0.011483989587496524 1101 46-47 0.07251057039979969 1101 48-49 0.020248525026914876 1101 50-51 -0.009297425867551112 1101 52-53 -0.05050381605905585 1101 54-55 -0.03592381171909409 1101 56-57 0.008847977134252005 1101 58-59 -0.10870873208531151 1101 60-61 -0.11153227402248689 1101 62-63 -0.06235168366448818 1101 64-65 -0.19547069868532674 1101 66-67 -0.2356940371184919 1101 68-69 -0.27342739489090206 1101 70-71 -0.24811581673662175 1101 72-73 -0.2117486155305528 1101 74-75 -0.0884770576661964 1101 76-77 -0.021322013433426434 1101 78-79 -0.11034345648435107 1101 80-81 -0.10480388170623556 1101 82-83 -0.08494760389784162 1101 84-85 -0.04606209225113744 1101 86-87 -0.22432524375934904 1101 88-89 -0.15226698441136932 1101 90-91 -0.1441559553794889 1101 92-93 -0.37513789522731145 1101 94-95 -0.3437936181524357 1101 96-97 -0.30760110534999896 1101 98-99 -0.445899555918718 1101 100-101 -0.20591778947764183 1101 102-103 -0.3337039683558345 1101 104-105 -0.3151749769587475 1101 106-107 -0.24516161159409222 1101 108-109 -0.30435808121671926 1101 110-111 -0.3275424757176921 1101 112-113 -0.37415658074271363 1101 114-115 -0.4171813797516535 1101 116-117 -0.3943337543242613 1101 118-119 -0.48901685915508253 1101 120-121 -0.547848648816192 1101 122-123 -0.5895745666296754 1101 124-125 -0.463898327370714 1102 1 0.19444300689721672 1102 2 0.26381190197182036 1102 3 0.2541559286605164 1102 4 0.43987916108235225 1102 5 0.31361197995179424 1102 6 0.41782958132322534 1102 7 0.3434461272527898 1102 8 0.41498811505046973 1102 9 0.4526433991679042 1102 10-11 0.5943572622649285 1102 12-13 0.5515583957789474 1102 14-15 0.5644283529350531 1102 16-17 0.5242733948489331 1102 18-19 0.45570918495116075 1102 20-21 0.38189530873968636 1102 22-23 0.40970153629749717 1102 24-25 0.4011830689931486 1102 26-27 0.3670506368544295 1102 28-29 0.3748094725917781 1102 30-31 0.2354270923388455 1102 32-33 0.38411756169604416 1102 34-35 0.5157630658284873 1102 36-37 0.4488021959187307 1102 38-39 0.3884032181528383 1102 40-41 0.3320928776792087 1102 42-43 0.19639676933307015 1102 44-45 0.22628464223000577 1102 46-47 0.178616754112511 1102 48-49 0.15918885378923164 1102 50-51 0.24483991514878767 1102 52-53 0.2212522969176689 1102 54-55 0.20841312585110927 1102 56-57 0.1470309829752594 1102 58-59 0.08670125601214806 1102 60-61 0.09277164357919787 1102 62-63 0.08429502728477445 1102 64-65 0.06473359391057443 1102 66-67 0.055698570700961625 1102 68-69 0.1557590970475644 1102 70-71 0.2309939338021394 1102 72-73 0.17180578619665 1102 74-75 0.23041003944145189 1102 76-77 0.32141940201468344 1102 78-79 0.1455736057032624 1102 80-81 0.16278437090341313 1102 82-83 0.0877190686757956 1102 84-85 -0.027110806057400794 1102 86-87 0.05799917890806228 1102 88-89 0.03397513839652788 1102 90-91 0.08690826422594711 1102 92-93 0.14858942323423818 1102 94-95 0.1390892101840926 1102 96-97 0.16881826597106198 1102 98-99 0.11717286650059933 1102 100-101 0.16743748825337335 1102 102-103 0.12760955496538173 1102 104-105 0.2053431333224367 1102 106-107 0.020048100647809264 1102 108-109 0.08304594280890143 1102 110-111 0.22594465545348896 1102 112-113 -0.06732339656040409 1102 114-115 -0.004470168615831938 1102 116-117 0.038754292033647886 1102 118-119 -0.11983018016253766 1102 120-121 -0.22122538854224416 1102 122-123 -0.24345239471515967 1102 124-125 -0.5370322905840652 1103 1 0.1537959923551 1103 2 0.1226573040562613 1103 3 0.08969540330650716 1103 4 0.21073284474029919 1103 5 0.3314063512537331 1103 6 0.1900630525878526 1103 7 0.3869481202793281 1103 8 0.22614896876260104 1103 9 0.22723537219298606 1103 10-11 0.2185367229374222 1103 12-13 0.2661974818820667 1103 14-15 0.3479061201757503 1103 16-17 0.35857974661835357 1103 18-19 0.2322201401894617 1103 20-21 0.2056432238696928 1103 22-23 0.28759151971346597 1103 24-25 0.3586360579633947 1103 26-27 0.3862561222102556 1103 28-29 0.35680151187703757 1103 30-31 0.3715734931673822 1103 32-33 0.3700906703143261 1103 34-35 0.5044399790912522 1103 36-37 0.41398047662837456 1103 38-39 0.4005137378585957 1103 40-41 0.5050606693450419 1103 42-43 0.49380792325698764 1103 44-45 0.2979835229265504 1103 46-47 0.40337902909606527 1103 48-49 0.47917878186181184 1103 50-51 0.33666295299777715 1103 52-53 0.41789267784727 1103 54-55 0.4019189634870841 1103 56-57 0.33212804275087393 1103 58-59 0.31637323963126107 1103 60-61 0.29293363928273664 1103 62-63 -0.07300408237962586 1103 64-65 0.02445632637417816 1103 66-67 0.14063904570483032 1103 68-69 0.3036284714584099 1103 70-71 0.13331854604243176 1103 72-73 0.09823348902827433 1103 74-75 0.16729579188847765 1103 76-77 0.1100745766216562 1103 78-79 0.12772085329515903 1103 80-81 0.08322824767466486 1103 82-83 0.1253905453760389 1103 84-85 0.21118045965447152 1103 86-87 0.152172174940997 1103 88-89 0.0037804777521337485 1103 90-91 0.02338226374940433 1103 92-93 0.17181703455466746 1103 94-95 0.21549631350346132 1103 96-97 0.11927335381326643 1103 98-99 0.2636453661517386 1103 100-101 0.230568110994664 1103 102-103 0.3328658954857602 1103 104-105 0.2898763645125655 1103 106-107 0.22033374088919544 1103 108-109 0.2401357348487565 1103 110-111 0.2099790841682605 1103 112-113 0.21095370580018624 1103 114-115 0.20600724643965762 1103 116-117 0.11319233828857023 1103 118-119 0.20117714547967935 1103 120-121 0.003929416317721035 1103 122-123 -0.13923049145313016 1103 124-125 -0.24699115800959248 1104 1 0.27455748963429016 1104 2 0.36425722493205015 1104 3 0.30506711310380297 1104 4 0.45100120769331653 1104 5 0.5215041026242133 1104 6 0.42060664691508975 1104 7 0.4830400801970569 1104 8 0.2588866115570809 1104 9 0.45791725073365797 1104 10-11 0.2855047453999049 1104 12-13 0.3891106265749684 1104 14-15 0.6429721211245081 1104 16-17 0.5240491711545516 1104 18-19 0.3736835168518482 1104 20-21 0.49503270364631646 1104 22-23 0.4215467962177897 1104 24-25 0.25787311373679955 1104 26-27 0.49608034557498115 1104 28-29 0.4906244567996225 1104 30-31 0.32300551319753623 1104 32-33 0.4578938195312361 1104 34-35 0.3861918660754995 1104 36-37 0.3535383700927568 1104 38-39 0.2526856242201134 1104 40-41 0.32693228664760454 1104 42-43 0.31158962468397533 1104 44-45 0.2486893218887829 1104 46-47 0.34894038058156696 1104 48-49 0.30633450453877487 1104 50-51 0.2491098143124546 1104 52-53 0.2204535000064496 1104 54-55 0.33596929894077476 1104 56-57 0.34835583256619174 1104 58-59 0.37791063040046424 1104 60-61 0.4498134162696772 1104 62-63 0.40929050145815893 1104 64-65 0.3576301597041507 1104 66-67 0.3876137351148188 1104 68-69 0.400647210343017 1104 70-71 0.3703999615599258 1104 72-73 0.30714155658422726 1104 74-75 0.404216944244709 1104 76-77 0.4792549633412406 1104 78-79 0.4851516608200157 1104 80-81 0.4190088757963615 1104 82-83 0.47647264224092467 1104 84-85 0.5564047090276105 1104 86-87 0.6576875171623726 1104 88-89 0.6150855327269795 1104 90-91 0.6712703615996816 1104 92-93 0.6260815914865532 1104 94-95 0.7508847089853248 1104 96-97 0.5594933630810566 1104 98-99 0.6404703056638432 1104 100-101 0.6673671886114896 1104 102-103 0.6133571066539929 1104 104-105 0.47828585795276624 1104 106-107 0.5463346530155313 1104 108-109 0.6073673215273168 1104 110-111 0.40619247777275547 1104 112-113 0.48834771690322043 1104 114-115 0.43798130395490276 1104 116-117 0.33001191127424434 1104 118-119 0.3697126332383185 1104 120-121 0.3770275504882079 1104 122-123 0.2741971804085104 1104 124-125 0.37104357605429783 1105 1 0.10252952001864912 1105 2 0.16048487994126148 1105 3 0.22346520752713062 1105 4 0.2530940993817694 1105 5 0.24274883331144537 1105 6 0.3256805380309018 1105 7 0.28307440403469997 1105 8 0.27388043671593465 1105 9 0.2543777558977567 1105 10-11 0.4642288817845497 1105 12-13 0.44185592990110223 1105 14-15 0.42702990590886714 1105 16-17 0.42067706970777863 1105 18-19 0.3102374552966225 1105 20-21 0.29210250004903315 1105 22-23 0.3122848794643076 1105 24-25 0.49127132889724123 1105 26-27 0.4703995607147249 1105 28-29 0.3757887972644909 1105 30-31 0.377059520634937 1105 32-33 0.4300975478934248 1105 34-35 0.5460016706193542 1105 36-37 0.46208923146584624 1105 38-39 0.45580297034927497 1105 40-41 0.5192095956587295 1105 42-43 0.4734567034666739 1105 44-45 0.4269020503406509 1105 46-47 0.4560018633713554 1105 48-49 0.30040760568888203 1105 50-51 0.3301399433976755 1105 52-53 0.3937828432655195 1105 54-55 0.45730708344917304 1105 56-57 0.4332948258256124 1105 58-59 0.36611720125935676 1105 60-61 0.32669346566840574 1105 62-63 0.2850429524216338 1105 64-65 0.29064046375471264 1105 66-67 0.4229690127067016 1105 68-69 0.4074037456706918 1105 70-71 0.2475870281999164 1105 72-73 0.3949221841412722 1105 74-75 0.32426094429990115 1105 76-77 0.39971464404331414 1105 78-79 0.40256833496088973 1105 80-81 0.5239777256200888 1105 82-83 0.48941091797690106 1105 84-85 0.5140384857536375 1105 86-87 0.539620414922183 1105 88-89 0.6624351805195587 1105 90-91 0.6746872220206726 1105 92-93 0.6752642874428929 1105 94-95 0.8112541300680967 1105 96-97 0.6767666187746748 1105 98-99 0.8214793919943304 1105 100-101 0.8620920114728889 1105 102-103 0.7739253990172017 1105 104-105 0.8354046908732933 1105 106-107 0.8079957025315458 1105 108-109 0.757438815148948 1105 110-111 0.6689078210456287 1105 112-113 0.7666683325935253 1105 114-115 0.8226989170855816 1105 116-117 0.8450531688720595 1105 118-119 0.8822089248840008 1105 120-121 0.8870172533369143 1105 122-123 0.9529428769926831 1105 124-125 0.8435870931610197 1106 1 -0.2201171444470127 1106 2 -0.13283984027273021 1106 3 -0.18006757510558913 1106 4 -0.8015913477031305 1106 5 -0.38174583374151894 1106 6 -0.36180051198358143 1106 7 -0.16469185589509294 1106 8 -0.17433809620145269 1106 9 -0.029040754111207434 1106 10-11 -0.09646623737295101 1106 12-13 -0.13094022889472257 1106 14-15 -0.1589495472534992 1106 16-17 -0.2554083012402941 1106 18-19 -0.20050971315443888 1106 20-21 -0.10699640708423885 1106 22-23 -0.08799271676793552 1106 24-25 -0.001071613622812606 1106 26-27 -0.11379396159067312 1106 28-29 -0.24129431989862837 1106 30-31 -0.17436989782790846 1106 32-33 -0.16726916211958098 1106 34-35 -0.19188002419996764 1106 36-37 -0.1522562444567228 1106 38-39 -0.21241941994293967 1106 40-41 -0.25873026298280166 1106 42-43 -0.4050612560662685 1106 44-45 -0.31178228164288413 1106 46-47 -0.28418078080013487 1106 48-49 -0.255100270767322 1106 50-51 -0.31134317511300225 1106 52-53 -0.28616706139499826 1106 54-55 -0.19746250157141532 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-0.9585210434080018 2216 94-95 -1.0086428422302305 2216 96-97 -1.0867711835983194 2216 98-99 -1.0524757680309769 2216 100-101 -1.115005820945786 2216 102-103 -1.0400596394596704 2216 104-105 -1.3636573570827366 2216 106-107 -1.3564143443765069 2216 108-109 -1.3174372603498838 2216 110-111 -1.4932824199608774 2216 112-113 -1.6779084636672081 2216 114-115 -1.6576163535345927 2216 116-117 -1.6419345843074034 2216 118-119 -1.6994098349930304 2216 120-121 -1.39402954462399 2216 122-123 -1.3501392789940176 2216 124-125 -1.2839262364746702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15009.0 3 3124.0 4 2107.0 5 1656.0 6 1856.0 7 2101.0 8 2411.0 9 3050.0 10 3809.0 11 4550.0 12 4183.0 13 3637.0 14 3626.0 15 3605.0 16 3486.0 17 3322.0 18 3192.0 19 3043.0 20 2875.0 21 3059.0 22 3721.0 23 5006.0 24 7835.0 25 11996.0 26 14397.0 27 11875.0 28 13183.0 29 17335.0 30 19212.0 31 23839.0 32 32411.0 33 44095.0 34 71820.0 35 103489.0 36 217195.0 37 329566.0 38 55527.0 39 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.82709443021221 10.75217639462761 7.170487880264638 14.250241294895547 2 11.520530072205105 31.541966258836275 42.45311781002464 14.484385858933985 3 36.973071435536845 19.97881161365941 19.525978495397347 23.5221384554064 4 14.571273293983754 15.491860242725158 46.63739118743692 23.299475275854167 5 16.6592981303483 33.54410187324918 27.990623813657763 21.80597618274475 6 31.395326821454738 38.16530124223014 14.714870219988905 15.724501716326209 7 43.365252171443444 23.928999124322534 16.42240787636381 16.28334082787021 8 40.84974250227204 25.243108149045746 15.551916086034534 18.355233262647683 9 53.61119578614936 11.504402996802124 11.0665646779498 23.817836539098725 10-11 39.13370493578687 25.34965531474185 19.238095057775727 16.278544691695558 12-13 29.014196374982724 28.81676679358456 23.405795180654458 18.763241650778266 14-15 25.20576034292444 28.987358069383884 21.816513084028983 23.990368503662694 16-17 23.61548423498049 32.794610754634455 21.439120120114435 22.150784890270618 18-19 23.65642803452722 26.28785999776584 25.196388236207383 24.859323731499554 20-21 24.310108811105664 27.681842537247025 22.447994274494807 25.560054377152507 22-23 25.314296912127503 24.178567979960732 22.572671694155027 27.93446341375674 24-25 22.584741831593654 23.776230065339675 31.674170663223283 21.96485743984338 26-27 19.542788115861217 23.097549927081445 30.7847418692616 26.57492008779574 28-29 22.58645696843923 24.430673478198305 23.846909059236086 29.13596049412638 30-31 22.971743571586803 27.437552422263582 22.302963289741992 27.287740716407626 32-33 28.87787482273465 24.738527689368624 20.64523641375863 25.7383610741381 34-35 27.695048687621053 20.064402466662752 26.010199502805005 26.230349342911186 36-37 24.778477477596876 25.721652217213247 27.83340559637839 21.666464708811482 38-39 22.93539115765398 29.05073812440478 24.73947436681479 23.27439635112645 40-41 21.23435378850847 28.62804664469113 26.086785708199933 24.05081385860047 42-43 21.99434644229149 22.939130533566342 23.821291911777234 31.245231112364934 44-45 23.55172550898113 24.161101781078948 23.034176949161527 29.252995760778393 46-47 25.05646930966387 22.427262038423745 24.014887449518422 28.50138120239396 48-49 19.071858498228767 23.022059477850586 26.277351878113386 31.62873014580726 50-51 23.356389673448195 24.85152540575798 25.110252474261014 26.681832446532816 52-53 24.802144413751055 22.33822702461172 27.96982181637108 24.88980674526614 54-55 21.689942309476432 24.23948667362159 27.776368280246817 26.29420273665516 56-57 21.16791983508824 25.960821624077873 25.966123035341955 26.905135505491927 58-59 22.89179666125798 23.42875210872401 30.14632793285406 23.533123297163943 60-61 25.17825936311328 28.081719090508045 27.176837453589137 19.563184092789538 62-63 26.394077207225802 25.898586231901895 25.224267886997005 22.4830686738753 64-65 29.8397624932903 20.991577259262694 27.167575335579524 22.001084911867476 66-67 29.869022131951056 24.478864665985373 23.73647845570036 21.91563474636321 68-69 24.03965679002294 28.543127989904026 24.937870587154904 22.479344632918128 70-71 24.69907010698362 28.34904932381316 22.886768174385477 24.06511239481775 72-73 22.935329058447476 28.5727065877957 23.276985433926793 25.214978919830035 74-75 21.611704965417278 28.863354810551243 25.072580878033357 24.452359345998122 76-77 22.980460057927328 28.433098383213096 22.866858495527175 25.719583063332397 78-79 23.72965033581413 29.740555242359395 20.356220004761845 26.173574417064632 80-81 22.74688634840332 23.852900079142984 24.80782265912852 28.59239091332518 82-83 24.79575434098943 20.37330331734711 27.496435759415185 27.334506582248284 84-85 26.08394515131488 21.830681859068193 30.305725934580767 21.77964705503616 86-87 27.80312572629801 23.827071609019608 24.624336776015493 23.745465888666885 88-89 23.505732597455104 25.892697364870433 26.701860431206466 23.899709606468004 90-91 20.75225829904783 27.992352739589858 26.2547736210223 25.00061534034001 92-93 23.91184351300429 23.636987663204536 26.74026453573694 25.71090428805423 94-95 23.079701585946 23.382300518801895 27.4343899521248 26.103607943127304 96-97 22.675159476874196 22.137132123915755 27.731603592276944 27.4561048069331 98-99 21.796210531896364 24.907412316244688 28.56419980762968 24.73217734422927 100-101 20.677929357547598 28.998470633174904 25.581417364727244 24.74218264455025 102-103 21.99852223580705 27.55121409073245 25.52963078142107 24.920632892039432 104-105 22.46676079845085 24.60616829835263 25.39626940876372 27.5308014944328 106-107 21.589393315440944 26.527314295451642 23.258781708107904 28.62451068099951 108-109 23.675456251195182 26.578016635016084 25.030057008915808 24.71647010487293 110-111 25.125340093872534 27.49619909005364 22.95740140827737 24.421059407796456 112-113 22.333842020459826 28.511361205096215 24.276588211459853 24.878208562984113 114-115 23.618568574183552 25.55278321752471 25.57891293738287 25.249735270908864 116-117 26.732940399081347 24.656782091892087 23.80136435153542 24.80891315749115 118-119 23.660205272160646 26.113248803558037 25.199296464178268 25.027249460103047 120-121 20.98848063254278 26.534968967384515 26.596598719312926 25.879951680759778 122-123 22.23685437531094 27.284600058602248 27.6407578150924 22.837787750994416 124-125 23.481946399837906 28.475258123799772 23.223051514672687 24.819743961689632 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 5.5 2 12.5 3 18.5 4 19.5 5 28.5 6 40.5 7 47.5 8 50.0 9 51.0 10 72.0 11 94.0 12 108.0 13 133.5 14 190.5 15 250.5 16 280.0 17 323.5 18 409.0 19 492.0 20 606.0 21 755.5 22 897.0 23 1112.5 24 1355.5 25 1591.5 26 2019.5 27 2479.5 28 2744.0 29 3177.5 30 3961.0 31 4573.0 32 5265.0 33 6150.0 34 7235.0 35 8201.5 36 9033.5 37 10070.5 38 10818.5 39 11241.5 40 11611.5 41 12589.0 42 13540.5 43 14720.0 44 16303.0 45 20116.0 46 34974.5 47 62543.0 48 85457.0 49 100727.0 50 101230.0 51 81743.5 52 61392.0 53 48368.0 54 41039.5 55 36600.0 56 32936.5 57 28884.5 58 25423.0 59 21795.5 60 18941.0 61 16391.0 62 13213.5 63 10284.5 64 7926.0 65 6842.5 66 6055.0 67 5223.0 68 4701.5 69 4220.5 70 3455.0 71 2536.0 72 1888.0 73 1479.5 74 1161.0 75 943.0 76 710.5 77 527.5 78 429.5 79 304.0 80 290.0 81 322.5 82 270.5 83 130.0 84 36.5 85 30.0 86 27.0 87 19.0 88 10.5 89 9.5 90 9.5 91 10.0 92 8.0 93 3.5 94 2.5 95 1.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15146839138674992 2 0.015904181095608743 3 0.008236093781654527 4 0.014389497181741241 5 0.003124035572351717 6 0.0 7 9.46677446167187E-5 8 5.680064677003122E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.940113184755464E-4 28-29 0.00738408408010406 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.733387230835935E-5 52-53 0.0 54-55 0.0 56-57 3.313371061585155E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0017986871477176552 66-67 2.3666936154179675E-4 68-69 0.005964067910853278 70-71 0.017324197264859523 72-73 1.4200161692507805E-4 74-75 0.008567430887813043 76-77 2.3666936154179675E-4 78-79 0.0011833468077089837 80-81 9.46677446167187E-4 82-83 0.0 84-85 0.01741886500947624 86-87 0.002603362976959764 88-89 0.016377519818692333 90-91 0.0 92-93 0.012874813267873745 94-95 0.013490153607882414 96-97 0.005680064677003122 98-99 0.002840032338501561 100-101 6.153403400086715E-4 102-103 4.733387230835935E-5 104-105 5.206725953919529E-4 106-107 0.0010413451907839058 108-109 0.0 110-111 0.0 112-113 0.0011360129354006244 114-115 0.005443395315461326 116-117 0.0 118-119 0.07578152956568332 120-121 0.0017040194031009365 122-123 0.0049700565923777315 124-125 0.2476981537896445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1056326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.61618031902566 #Duplication Level Percentage of deduplicated Percentage of total 1 81.99694025621301 37.403872123357864 2 9.739727649120038 8.88578345400919 3 2.8544997767027906 3.906341296040789 4 1.3528810422323696 2.4685306229065254 5 0.7601857809667512 1.7338385830269327 6 0.5441735244408478 1.4893870569440057 7 0.36789776991036105 1.1747463707838908 8 0.2905717056929028 1.0603817057995448 9 0.23832741987165218 0.9784427903850081 >10 1.5743787775782052 13.886423230364741 >50 0.15691930459505854 4.981632745459212 >100 0.10813770285842282 9.459954018649716 >500 0.007887202879308152 2.468024031642983 >1k 0.006226739115243279 5.159966306077068 >5k 8.30231882032437E-4 2.9257072391069103 >10k+ 4.151159410162185E-4 2.016968425445601 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 10694 1.0123768609311898 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10609 1.0043301026387685 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9192 0.8701859085168783 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8402 0.7953983902696705 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7045 0.6669342608247832 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6262 0.5928094167898925 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4560 0.43168491545223725 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3581 0.33900519347246966 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3106 0.2940380147795283 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2780 0.263176330034478 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2764 0.2616616461206105 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2654 0.25124819421277145 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2068 0.19577289586737429 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2050 0.19406887646427334 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1992 0.18857814727650365 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1984 0.1878208053195699 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1944 0.18403409553490116 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1851 0.17522999528554634 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1806 0.17096994677779398 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1743 0.16500587886694068 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1651 0.15629644636220258 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1573 0.14891236228209853 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1441 0.13641621999269166 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1322 0.1251507583833021 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1281 0.12126938085401666 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1237 0.11710400009088102 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1220 0.1154946484323968 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1185 0.11218127737081167 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1173 0.11104526443541105 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1172 0.11095059669079432 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1152 0.10905724179845994 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1061 0.10044247703833854 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1059 0.10025314154910511 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.46677446167187E-5 0.0 10-11 0.0 0.0 0.0 9.46677446167187E-5 0.0 12-13 0.0 0.0 0.0 9.46677446167187E-5 0.0 14-15 0.0 0.0 0.0 9.46677446167187E-5 0.0 16-17 0.0 0.0 0.0 1.893354892334374E-4 0.0 18-19 0.0 0.0 0.0 1.893354892334374E-4 0.0 20-21 0.0 0.0 0.0 8.046758292421089E-4 0.0 22-23 0.0 0.0 0.0 0.0027453645938848425 0.0 24-25 0.0 0.0 0.0 0.004496717869294138 0.0 26-27 0.0 0.0 0.0 0.006674075995478668 0.0 28-29 0.0 0.0 0.0 0.011596798715548041 0.0 30-31 0.0 2.840032338501561E-4 0.0 0.035405736486652795 0.0 32-33 0.0 2.840032338501561E-4 0.0 0.08794633474893167 0.0 34-35 0.0 2.840032338501561E-4 0.0 0.14403697343433752 0.0 36-37 0.0 2.840032338501561E-4 0.0 0.21002039143219042 0.0 38-39 0.0 2.840032338501561E-4 0.0 0.32537303824766217 0.0 40-41 0.0 2.840032338501561E-4 0.0 0.4974789979608568 0.0 42-43 0.0 2.840032338501561E-4 0.0 0.8548024000166614 0.0 44-45 0.0 2.840032338501561E-4 0.0 1.3514293882759678 0.0 46-47 0.0 2.840032338501561E-4 0.0 1.861215193036998 0.0 48-49 0.0 2.840032338501561E-4 0.0 2.0783356653154423 0.0 50-51 0.0 2.840032338501561E-4 0.0 2.3526827892146933 0.0 52-53 0.0 2.840032338501561E-4 0.0 2.5768560084670833 0.0 54-55 0.0 2.840032338501561E-4 0.0 2.8024965777610324 0.0 56-57 0.0 3.786709784668748E-4 0.0 3.1348750291103316 0.0 58-59 0.0 3.786709784668748E-4 0.0 3.516717377021866 0.0 60-61 0.0 3.786709784668748E-4 0.0 4.034691941692243 0.0 62-63 0.0 3.786709784668748E-4 0.0 4.672799874281235 0.0 64-65 0.0 3.786709784668748E-4 0.0 5.269774671834263 0.0 66-67 0.0 3.786709784668748E-4 0.0 5.772933734472123 0.0 68-69 0.0 3.786709784668748E-4 0.0 6.199506591715057 0.0 70-71 0.0 4.260048507752341E-4 0.0 6.702050314012909 0.0 72-73 0.0 4.733387230835935E-4 0.0 7.1735903499487845 0.0 74-75 0.0 4.733387230835935E-4 0.0 7.659803886300252 0.0 76-77 0.0 4.733387230835935E-4 0.0 8.23713512684531 0.0 78-79 0.0 4.733387230835935E-4 0.0 8.983022286680438 0.0 80-81 0.0 4.733387230835935E-4 0.0 10.559429570038038 0.0 82-83 0.0 4.733387230835935E-4 0.0 11.710589344577336 0.0 84-85 0.0 4.733387230835935E-4 0.0 12.27599244930069 0.0 86-87 0.0 5.206725953919529E-4 0.0 12.766655369649143 0.0 88-89 0.0 5.680064677003122E-4 0.0 13.349714008743513 0.0 90-91 0.0 5.680064677003122E-4 0.0 14.03354646198238 0.0 92-93 0.0 5.680064677003122E-4 0.0 14.898998983268424 0.0 94-95 0.0 5.680064677003122E-4 0.0 16.047555394830763 0.0 96-97 0.0 5.680064677003122E-4 0.0 17.375365180824858 0.0 98-99 0.0 5.680064677003122E-4 0.0 18.693376855251124 0.0 100-101 0.0 5.680064677003122E-4 0.0 19.837578550561098 0.0 102-103 0.0 5.680064677003122E-4 0.0 20.8596588553155 0.0 104-105 0.0 5.680064677003122E-4 0.0 21.955532666998636 0.0 106-107 0.0 5.680064677003122E-4 0.0 22.99545784161329 0.0 108-109 0.0 5.680064677003122E-4 0.0 23.867489771150197 0.0 110-111 0.0 5.680064677003122E-4 0.0 24.823965328885212 0.0 112-113 0.0 5.680064677003122E-4 0.0 27.339050633989885 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTTAG 1120 0.0 89.39814 1 GAGTAGT 1820 0.0 89.39813 1 AGTAGTA 1530 0.0 88.675064 2 AGTCCGT 235 0.0 88.62541 2 GAGTAAG 1145 0.0 82.24108 1 GAGTCGG 730 0.0 81.64213 1 GAGTACG 525 0.0 80.60023 1 GAGTCAG 4300 0.0 80.38902 1 GAGTCCG 680 0.0 79.757164 1 GAGTCGA 410 0.0 78.495926 1 GAGTAGG 1105 0.0 73.89168 1 GAGTCGT 690 0.0 73.41876 1 AGTCGTA 430 0.0 73.344154 2 AGTTAGT 875 0.0 72.76689 2 GAGTGGT 785 0.0 71.36667 1 GTGGGGT 5575 0.0 70.74624 7 TGGGGTA 5825 0.0 70.36521 8 GAGTTAC 705 0.0 69.32054 1 TAAGCGG 240 0.0 66.94067 4 GAGTAAC 825 0.0 66.46164 1 >>END_MODULE