##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1056326 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59276208291758 33.0 31.0 34.0 31.0 34.0 2 32.678918250615816 34.0 31.0 34.0 31.0 34.0 3 32.85718897385845 34.0 31.0 34.0 31.0 34.0 4 36.10970950255887 37.0 35.0 37.0 35.0 37.0 5 36.13768098106077 37.0 35.0 37.0 35.0 37.0 6 36.238693357921704 37.0 37.0 37.0 35.0 37.0 7 36.26121102765624 37.0 37.0 37.0 35.0 37.0 8 36.26329182468291 37.0 37.0 37.0 35.0 37.0 9 38.09433451415567 39.0 39.0 39.0 35.0 39.0 10-11 37.856926744205865 39.0 38.0 39.0 35.0 39.0 12-13 37.75160130490019 39.0 38.0 39.0 35.0 39.0 14-15 39.288852115729426 41.0 39.0 41.0 36.0 41.0 16-17 39.34387821562662 41.0 39.0 41.0 36.0 41.0 18-19 39.33809070305948 41.0 39.0 41.0 35.0 41.0 20-21 39.32957297273758 41.0 39.0 41.0 35.0 41.0 22-23 39.18944625049464 41.0 39.0 41.0 35.0 41.0 24-25 38.986507953037226 41.0 38.0 41.0 35.0 41.0 26-27 38.870206735420695 41.0 38.0 41.0 35.0 41.0 28-29 38.76074999573995 40.5 38.0 41.0 35.0 41.0 30-31 38.64029901753814 40.0 38.0 41.0 35.0 41.0 32-33 38.4799129246085 40.0 38.0 41.0 35.0 41.0 34-35 38.1052501784487 40.0 37.0 41.0 34.0 41.0 36-37 37.876397059241185 40.0 37.0 41.0 33.5 41.0 38-39 37.45940173772112 40.0 37.0 41.0 33.0 41.0 40-41 37.184101782972306 40.0 37.0 41.0 33.0 41.0 42-43 37.005199152534345 40.0 36.5 41.0 32.0 41.0 44-45 36.828488080384275 40.0 36.0 41.0 32.0 41.0 46-47 36.63749732563622 40.0 35.5 41.0 31.0 41.0 48-49 36.41074204364941 40.0 35.0 41.0 31.0 41.0 50-51 36.13948866164423 39.5 35.0 41.0 30.5 41.0 52-53 35.8636656676064 39.0 35.0 41.0 30.0 41.0 54-55 35.61364436736386 39.0 35.0 41.0 29.5 41.0 56-57 35.363295516724946 38.5 35.0 41.0 29.0 41.0 58-59 35.04948330344988 37.0 35.0 40.0 29.0 41.0 60-61 34.750229095941975 37.0 35.0 40.0 29.0 41.0 62-63 34.358000749768536 36.5 35.0 39.0 27.5 41.0 64-65 33.95600269235065 36.0 34.5 39.0 27.0 41.0 66-67 33.54028301868931 35.0 34.0 37.5 27.0 40.5 68-69 33.20172655032632 35.0 34.0 37.0 26.5 39.5 70-71 33.00654296116919 35.0 34.0 36.5 26.0 39.0 72-73 32.7186327894987 35.0 34.0 36.0 26.0 39.0 74-75 32.4250752135231 35.0 34.0 35.5 26.0 37.5 76-77 32.26478520835424 35.0 34.0 35.0 26.0 37.0 78-79 32.26173501362268 35.0 34.0 35.0 26.5 37.0 80-81 32.181882297699765 35.0 34.0 35.0 27.0 36.0 82-83 32.078158636633006 35.0 34.0 35.0 27.0 36.0 84-85 31.900734716365967 35.0 34.0 35.0 26.0 36.0 86-87 31.797725796771072 35.0 34.0 35.0 25.5 35.0 88-89 31.737991869934092 35.0 34.0 35.0 25.0 35.0 90-91 31.728655736959993 35.0 34.0 35.0 25.0 35.0 92-93 31.721360167221107 35.0 34.0 35.0 25.0 36.0 94-95 31.63895142219353 35.0 34.0 35.0 25.0 35.0 96-97 31.60026024162995 35.0 34.0 35.0 25.0 35.0 98-99 31.525703239340885 35.0 34.0 35.0 25.0 35.0 100-101 31.488037783790233 35.0 33.5 35.0 25.0 35.0 102-103 30.81977296781486 34.5 32.0 35.0 23.5 35.0 104-105 31.36904894890403 35.0 33.0 35.0 24.0 35.0 106-107 31.46057751110926 35.0 34.0 35.0 25.0 35.0 108-109 31.40059934149117 35.0 33.5 35.0 24.0 35.0 110-111 31.321840511357287 35.0 33.0 35.0 24.0 35.0 112-113 31.291640554147108 35.0 33.0 35.0 24.0 35.0 114-115 31.195604387281957 35.0 33.0 35.0 23.0 35.0 116-117 31.078979879317558 35.0 33.0 35.0 20.0 35.0 118-119 30.931334644797154 35.0 33.0 35.0 18.5 35.0 120-121 30.695741182172927 35.0 33.0 35.0 4.5 35.0 122-123 30.408219621594093 35.0 33.0 35.0 2.0 35.0 124-125 28.205611241226666 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15704426216495904 1101 2 0.12665479612435604 1101 3 0.15423399796494408 1101 4 0.1639526956014734 1101 5 0.08664629042726801 1101 6 0.16613762957568667 1101 7 0.17938902110984145 1101 8 0.2223077804358553 1101 9 0.24598233200454445 1101 10-11 0.16161210340519006 1101 12-13 0.1849154897465084 1101 14-15 0.2618488299807282 1101 16-17 0.15464558730415945 1101 18-19 0.1935260509628023 1101 20-21 0.22668741149107063 1101 22-23 0.2886181103810159 1101 24-25 0.37184681328916014 1101 26-27 0.35175075830296265 1101 28-29 0.3932557982584868 1101 30-31 0.3039969197136827 1101 32-33 0.329251965733512 1101 34-35 0.4233088565164209 1101 36-37 0.3789995622159452 1101 38-39 0.2435738179511162 1101 40-41 0.2832656585938125 1101 42-43 0.27024319156625154 1101 44-45 0.19207143345443 1101 46-47 0.25705713245889683 1101 48-49 0.302736359630849 1101 50-51 0.30439791781078895 1101 52-53 0.23091100572361256 1101 54-55 0.3461853044958758 1101 56-57 0.25244828800105523 1101 58-59 0.1545980567522065 1101 60-61 0.0820936840427251 1101 62-63 0.14161307060684436 1101 64-65 0.04653979313253842 1101 66-67 -0.18636244451581518 1101 68-69 -0.06742854922089236 1101 70-71 0.07887939286735701 1101 72-73 -0.0931692567460658 1101 74-75 -0.011201428239715483 1101 76-77 0.14055501009442395 1101 78-79 0.043922000889594415 1101 80-81 -0.076499779635256 1101 82-83 -0.021844003633333386 1101 84-85 0.029134306147795996 1101 86-87 0.0897036060160481 1101 88-89 -0.04666281971496389 1101 90-91 -0.1894624238773801 1101 92-93 -0.07558988706269432 1101 94-95 -0.11761023791513736 1101 96-97 -0.09260856697260778 1101 98-99 -0.11417655226092549 1101 100-101 -0.1455057324265745 1101 102-103 -0.20463629252708415 1101 104-105 -0.11860432773027796 1101 106-107 -0.10077838179770993 1101 108-109 -0.11347767732769043 1101 110-111 -0.10179455538847293 1101 112-113 0.021215581662424654 1101 114-115 -0.08570746758108427 1101 116-117 -0.16601394300163363 1101 118-119 -0.3211405914079357 1101 120-121 -0.11957309783607073 1101 122-123 -0.10128952070792252 1101 124-125 -0.14294063521117195 1102 1 0.03974215308092255 1102 2 0.09804782727673711 1102 3 0.13281285947502397 1102 4 0.12925613178501294 1102 5 0.19704708826216688 1102 6 0.09750847020659137 1102 7 0.11878300525356167 1102 8 0.14413984721613105 1102 9 0.17784689697089107 1102 10-11 0.21454964013916822 1102 12-13 0.07368500859417537 1102 14-15 0.09509897347089691 1102 16-17 0.11313164045311908 1102 18-19 0.025492412731303205 1102 20-21 0.13416135436475685 1102 22-23 0.10543653698049837 1102 24-25 0.21619960123185678 1102 26-27 0.30269459383713837 1102 28-29 0.2554968637185553 1102 30-31 0.27608918857144715 1102 32-33 0.2090985124689766 1102 34-35 0.3435445643536923 1102 36-37 0.41249307291592885 1102 38-39 0.5642662331048598 1102 40-41 0.5148009591454255 1102 42-43 0.4641459175349709 1102 44-45 0.4867362571461129 1102 46-47 0.45739230384474183 1102 48-49 0.45723441968308265 1102 50-51 0.3775695379649875 1102 52-53 0.3907686575647631 1102 54-55 0.4235978837846517 1102 56-57 0.5242053854729605 1102 58-59 0.5148959748402362 1102 60-61 0.39462168062456016 1102 62-63 0.5677306449288508 1102 64-65 0.5709220470161256 1102 66-67 0.5515619836647687 1102 68-69 0.5398459841530041 1102 70-71 0.5874403168751599 1102 72-73 0.5531131909846252 1102 74-75 0.6635043799865343 1102 76-77 0.5828389798546709 1102 78-79 0.5668900274394559 1102 80-81 0.5832190583847705 1102 82-83 0.672504944778126 1102 84-85 0.6021690814712315 1102 86-87 0.6295736920313892 1102 88-89 0.6554601949274286 1102 90-91 0.6428588484054387 1102 92-93 0.5339708902553184 1102 94-95 0.5514622888606695 1102 96-97 0.5260998934836678 1102 98-99 0.516085205318948 1102 100-101 0.4533206016382394 1102 102-103 0.44632669143844694 1102 104-105 0.49276619786216713 1102 106-107 0.5362692549708221 1102 108-109 0.4764254385011206 1102 110-111 0.48133474131769916 1102 112-113 0.40326764373681456 1102 114-115 0.35697473048162465 1102 116-117 0.36233779546918754 1102 118-119 0.3782723541130544 1102 120-121 0.5227036851093203 1102 122-123 0.5376397324835622 1102 124-125 0.24100731846636236 1103 1 0.0010625343522647768 1103 2 0.012801919412567031 1103 3 0.026895768240073892 1103 4 0.17585944308541457 1103 5 0.14083984767599844 1103 6 0.1176423575433958 1103 7 0.08789465366070459 1103 8 0.07530614983151196 1103 9 0.11427129628626176 1103 10-11 0.1829433907977318 1103 12-13 0.09964822564942466 1103 14-15 0.10228507060377012 1103 16-17 0.20160346041528499 1103 18-19 0.09810332022050972 1103 20-21 0.09501611071618754 1103 22-23 0.176947221672215 1103 24-25 0.16558958986889394 1103 26-27 0.1321396505712329 1103 28-29 0.13639489803146176 1103 30-31 0.22922458622272046 1103 32-33 0.2675539002856411 1103 34-35 0.22434078771316024 1103 36-37 0.1171418702176723 1103 38-39 0.14229827721250388 1103 40-41 0.1843707805751862 1103 42-43 0.1967801489880543 1103 44-45 0.24229100580832608 1103 46-47 0.24567773144600125 1103 48-49 0.13385696352349186 1103 50-51 0.16100529928783658 1103 52-53 0.14413577599677296 1103 54-55 0.11204592412552472 1103 56-57 0.015052604491344823 1103 58-59 0.01514017873554252 1103 60-61 0.05998094276332466 1103 62-63 0.12245984677843325 1103 64-65 0.1456304025390196 1103 66-67 0.08443315697311249 1103 68-69 0.12992755632296848 1103 70-71 0.09688618999545184 1103 72-73 0.06301163633848716 1103 74-75 -0.029943076543275993 1103 76-77 0.1275199864398715 1103 78-79 0.009176981493055791 1103 80-81 0.09379339556935662 1103 82-83 0.11434383570798445 1103 84-85 0.07579245024141912 1103 86-87 0.08763493618497264 1103 88-89 0.03319852986031435 1103 90-91 0.05643653751987898 1103 92-93 -0.006335805155728735 1103 94-95 -0.005479699305524832 1103 96-97 0.07016244005802363 1103 98-99 0.08380206739619567 1103 100-101 0.06228300325658154 1103 102-103 0.11025087004475864 1103 104-105 0.1759615998991464 1103 106-107 0.11758132049435588 1103 108-109 0.05816942928165503 1103 110-111 0.13967616731656207 1103 112-113 0.07466840107741035 1103 114-115 0.0824964344884691 1103 116-117 0.07550975962793416 1103 118-119 0.0489590108501865 1103 120-121 0.031057167043929468 1103 122-123 0.03482130152088203 1103 124-125 0.21033587878828186 1104 1 0.027527934909429064 1104 2 -0.00837296524374409 1104 3 0.05888704259778166 1104 4 0.07856963051089139 1104 5 0.18353944857494042 1104 6 0.08000615675105394 1104 7 0.0566900424774488 1104 8 0.054863896769568044 1104 9 0.15171334970609962 1104 10-11 0.2056760333815788 1104 12-13 0.1625368638155038 1104 14-15 0.23042187856454177 1104 16-17 0.2645717722672458 1104 18-19 0.24627027597139062 1104 20-21 0.19300146541062446 1104 22-23 0.28942540298754693 1104 24-25 0.34101868303582705 1104 26-27 0.31903213871633795 1104 28-29 0.28988625389443 1104 30-31 0.35918106790601456 1104 32-33 0.2942102429520901 1104 34-35 0.24459896708701478 1104 36-37 0.23321818845086284 1104 38-39 0.3685678819760696 1104 40-41 0.3896198112120004 1104 42-43 0.340745004650465 1104 44-45 0.3675032400233249 1104 46-47 0.32687986999762586 1104 48-49 0.32413008858187453 1104 50-51 0.2735251490250903 1104 52-53 0.36266970691328027 1104 54-55 0.3244211707018394 1104 56-57 0.34167121663067945 1104 58-59 0.27319408943140644 1104 60-61 0.3366876538518895 1104 62-63 0.2908387345337786 1104 64-65 0.398708371024874 1104 66-67 0.48752980756462705 1104 68-69 0.5179906065979765 1104 70-71 0.43243603916492646 1104 72-73 0.4201297017514989 1104 74-75 0.43554860167759557 1104 76-77 0.468818704151424 1104 78-79 0.5063064393273606 1104 80-81 0.5043747936868641 1104 82-83 0.46691088523570556 1104 84-85 0.4993547065269439 1104 86-87 0.5124119872329125 1104 88-89 0.5424470879497534 1104 90-91 0.49667028363006693 1104 92-93 0.392344450605961 1104 94-95 0.4762873897843036 1104 96-97 0.5433499552830199 1104 98-99 0.6120102153584703 1104 100-101 0.4909851280713653 1104 102-103 0.45748848261818154 1104 104-105 0.4799038905084565 1104 106-107 0.5338818239954222 1104 108-109 0.5190826179324581 1104 110-111 0.6567817413608914 1104 112-113 0.6520672658647513 1104 114-115 0.5347581597992388 1104 116-117 0.6471524295572557 1104 118-119 0.6099574595027839 1104 120-121 0.6132144740367131 1104 122-123 0.6363489299740976 1104 124-125 0.659302593503373 1105 1 0.07370384543977337 1105 2 0.14273471156713669 1105 3 0.14637143301332145 1105 4 0.13149905794098515 1105 5 0.15573800248923675 1105 6 0.13327192329376913 1105 7 0.215077305056532 1105 8 0.1554094560583934 1105 9 0.18086532669015298 1105 10-11 0.154216580355218 1105 12-13 0.21795951271886338 1105 14-15 0.21201809224854173 1105 16-17 0.2272006160196156 1105 18-19 0.1164567458250616 1105 20-21 0.2002711334870284 1105 22-23 0.284875769683417 1105 24-25 0.30904145750579204 1105 26-27 0.2442419625781369 1105 28-29 0.2620960191124837 1105 30-31 0.35887318825677283 1105 32-33 0.36673357971205434 1105 34-35 0.31367180951473017 1105 36-37 0.6162674733421696 1105 38-39 0.524896576704343 1105 40-41 0.4312749791055879 1105 42-43 0.39339026865079774 1105 44-45 0.4510856655285238 1105 46-47 0.5041366192024768 1105 48-49 0.5351971114805352 1105 50-51 0.45217856795529343 1105 52-53 0.4361184074369362 1105 54-55 0.362111849106455 1105 56-57 0.47086683145858643 1105 58-59 0.40883792873375313 1105 60-61 0.38536972197521635 1105 62-63 0.34684264641638407 1105 64-65 0.49054188488894823 1105 66-67 0.4956028086754074 1105 68-69 0.35791173076403027 1105 70-71 0.28073804409100234 1105 72-73 0.30761736931737005 1105 74-75 0.36869904702508194 1105 76-77 0.3097544513071995 1105 78-79 0.40084041130254633 1105 80-81 0.42915600078206495 1105 82-83 0.3381404375424779 1105 84-85 0.36662451258862916 1105 86-87 0.4400658753130564 1105 88-89 0.3341514130982617 1105 90-91 0.3073648325216958 1105 92-93 0.38694189580855465 1105 94-95 0.2926673632911303 1105 96-97 0.3054210797940371 1105 98-99 0.31255284246016046 1105 100-101 0.29800668137162845 1105 102-103 0.3145710944035045 1105 104-105 0.3531652760186219 1105 106-107 0.31109252061409265 1105 108-109 0.37231889012221586 1105 110-111 0.33161986738284455 1105 112-113 0.40301979547984246 1105 114-115 0.48894532302892557 1105 116-117 0.5009962681696756 1105 118-119 0.5799495425975216 1105 120-121 0.6394609985325488 1105 122-123 0.6284027028365315 1105 124-125 0.7916360797703881 1106 1 0.08091578881553829 1106 2 0.07836876966021578 1106 3 0.06970400273242916 1106 4 0.11101791359768498 1106 5 0.06849648029164257 1106 6 0.0957458986469959 1106 7 0.13892110327371654 1106 8 0.027690764803345758 1106 9 0.17809862880513805 1106 10-11 0.12507781929684825 1106 12-13 0.10935419796381751 1106 14-15 0.0277939130776943 1106 16-17 0.08612050053419296 1106 18-19 0.14334509082596725 1106 20-21 0.08993126339128565 1106 22-23 0.15282426951834083 1106 24-25 0.14900335540158238 1106 26-27 0.12872411421844276 1106 28-29 0.22812248508336097 1106 30-31 0.21132186327378122 1106 32-33 0.18510344300265302 1106 34-35 0.1791513367926001 1106 36-37 0.1276143035570314 1106 38-39 -0.037643428820629765 1106 40-41 0.003858476952871115 1106 42-43 -0.012802670837267272 1106 44-45 0.0053731629176354545 1106 46-47 0.012056028704087396 1106 48-49 -0.02819901227886845 1106 50-51 -0.05672329301750523 1106 52-53 -0.0556909877132199 1106 54-55 0.004912202165741064 1106 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2216 82-83 -0.3341829047190856 2216 84-85 -0.44884382238448595 2216 86-87 -0.5628813816945843 2216 88-89 -0.424697848441312 2216 90-91 -0.3498572797621904 2216 92-93 -0.6872161352795274 2216 94-95 -0.6825771759480261 2216 96-97 -0.700909626167995 2216 98-99 -0.7758749712566093 2216 100-101 -0.5803699738583568 2216 102-103 -0.6540756932448772 2216 104-105 -0.6223379473423165 2216 106-107 -0.7328674054701843 2216 108-109 -0.6504317251453386 2216 110-111 -0.6600146190952039 2216 112-113 -0.3811489801446939 2216 114-115 -0.4394202532043252 2216 116-117 -0.6163782116749132 2216 118-119 -0.5632936978208889 2216 120-121 -0.7269095778818695 2216 122-123 -0.7391412717221897 2216 124-125 -1.1583441661694032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 46.0 5 112.0 6 325.0 7 690.0 8 926.0 9 1674.0 10 4018.0 11 12919.0 12 19108.0 13 6126.0 14 5198.0 15 6408.0 16 3933.0 17 2405.0 18 2054.0 19 1876.0 20 1856.0 21 1969.0 22 2170.0 23 2516.0 24 3184.0 25 4075.0 26 5391.0 27 7042.0 28 9214.0 29 11864.0 30 14940.0 31 20135.0 32 26537.0 33 37214.0 34 66669.0 35 107261.0 36 211809.0 37 380584.0 38 73749.0 39 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 68.01212008522765 10.738171627844322 7.242832396373082 14.006875890554948 2 11.6641121935934 29.986452930322997 44.17461803606304 14.174816840020563 3 36.374282181826445 19.91336008012678 20.151354790093208 23.561002947953565 4 15.108778275053503 15.617174778832357 46.081631569868506 23.192415376245638 5 16.879234226470626 32.87170755451345 28.942932523215447 21.306125695800482 6 31.45931474272558 37.475385873763614 15.479539980914586 15.585759402596223 7 43.5454585042875 22.902967455122756 17.919562710754068 15.632011329835677 8 42.07299640451906 23.351976567839852 16.920155330835367 17.65487169680572 9 51.04901327809785 9.93007840382609 15.392975274678461 23.627933043397586 10-11 35.890056668111924 24.27697510048981 23.76752063283494 16.065447598563324 12-13 28.32960657978692 26.84526367806908 26.814259991707107 18.010869750436893 14-15 24.42494078532574 26.568502526682103 25.93366063128239 23.072896056709766 16-17 22.502522895394037 31.23832036700791 25.415922735973556 20.8432340016245 18-19 22.74704967973902 24.760348604502777 28.68390061401499 23.808701101743214 20-21 23.118431241870407 25.64738537156143 26.852269091170715 24.381914295397443 22-23 23.92694111476949 22.785579451798025 26.936002711284207 26.351476722148277 24-25 20.804704229565495 22.121721892673285 36.21893241291041 20.85464146485081 26-27 18.28010481612684 21.994441110036107 34.50994295321709 25.21551112061996 28-29 21.61288276535842 22.82836927236478 27.58102138923022 27.97772657304658 30-31 21.721750671667646 25.84798632240426 26.739425139587592 25.690837866340505 32-33 28.245494288694967 22.50015620177862 24.82656869186217 24.427780817664242 34-35 26.246158856262173 18.45282611618004 30.522868887067062 24.77814614049072 36-37 23.890541366964367 24.582515246240273 31.07478183818253 20.452161548612832 38-39 22.296194546001896 28.11267544299771 27.03256381079326 22.558566200207135 40-41 20.727739353192103 26.91342445419312 28.59235690497062 23.766479287644156 42-43 21.76293114057592 22.142454128744347 25.57382853399424 30.520786196685496 44-45 23.18233196948669 23.520106482279147 24.854637678141028 28.442923870093136 46-47 24.22940455881991 21.993352430973015 25.516033875905737 28.26120913430134 48-49 18.70369563941435 22.629803678031212 27.32821117723127 31.338289505323168 50-51 23.430077457148645 23.782241467122837 26.85809115746465 25.92958991826387 52-53 24.287009881419184 21.637259709597227 29.6674984805827 24.40823192840089 54-55 21.544674655362076 23.661161421758056 28.774686981102427 26.01947694177744 56-57 21.110765047911347 25.46907867457584 27.659264280155938 25.76089199735688 58-59 22.934397146335506 22.569973663433448 31.364796473815847 23.1308327164152 60-61 25.148439023559018 27.574536648723974 27.832411585059912 19.444612742657096 62-63 26.19442293382914 24.242800044683175 26.513358565442864 23.04941845604482 64-65 29.405316161866697 20.180796458669008 28.301963598358842 22.111923781105457 66-67 29.229612827858066 24.171183895880628 24.777720135639942 21.82148314062136 68-69 23.106976444771785 28.3108150324805 25.714836139600845 22.86737238314687 70-71 24.30565942710868 27.280924638795224 23.810878459869397 24.602537474226708 72-73 22.740517605360466 27.946296881833828 24.655646078956686 24.65753943384902 74-75 21.315010706921917 27.273114549864342 26.22727264121114 25.1846021020026 76-77 22.678746901998057 28.086925816461967 23.46226449031833 25.77206279122165 78-79 23.043880392984743 28.241281574059524 21.4961101023737 27.218727930582038 80-81 22.195752068963557 22.649021230188406 25.854707732272047 29.30051896857599 82-83 24.27673843112827 19.357187080503557 28.301632261252678 28.064442227115492 84-85 25.51806923241499 21.14910548448112 30.43776258465663 22.895062698447262 86-87 26.829690834079628 23.443472943011912 24.764703320755146 24.96213290215331 88-89 22.26665820968148 24.99271058366451 27.547225004402048 25.193406202251957 90-91 20.03079541732382 26.347311341385137 27.083637059013977 26.538256182277063 92-93 23.851112251331504 22.609260777449386 27.145265760759465 26.39436121045965 94-95 22.25984213206908 22.299223913829632 28.541898997089916 26.899034957011374 96-97 21.762315800235914 20.776445907797402 28.791301170282658 28.669937121684026 98-99 20.064639136024294 23.614253554300472 29.417575634794563 26.90353167488067 100-101 19.661638547190925 27.118758792266785 27.07838299918775 26.14121966135454 102-103 21.298065180635522 25.87099058434612 26.715852871178026 26.11509136384033 104-105 20.866427599055594 23.236813256513614 25.995620670134027 29.90113847429676 106-107 20.38764548065654 25.445033067443195 24.260644914543427 29.90667653735684 108-109 22.58545183967828 24.785151553592357 26.30750355477381 26.321893051955552 110-111 24.143542613059477 26.494501869000064 23.6044980964458 25.75745742149466 112-113 20.987283245749644 26.68421496076484 25.343621221917918 26.984880571567597 114-115 21.583557224244974 23.801071268311247 26.60406241302335 28.01130909442043 116-117 24.880285157063604 23.057107151648268 24.72364758676841 27.33896010451972 118-119 21.630455363059735 24.69232225693458 25.67580232888384 28.00142005112184 120-121 19.444061827708868 24.443197981677 27.693152422673943 28.41958776794019 122-123 20.099753716150172 25.791121190304096 28.77757912868627 25.331545964859465 124-125 21.10884527992432 26.438541078277865 24.54374961234401 27.908864029453806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 5.5 2 36.5 3 110.0 4 212.0 5 268.5 6 322.0 7 419.0 8 565.0 9 760.0 10 911.5 11 1048.5 12 1176.5 13 1304.5 14 1474.5 15 1610.0 16 1705.0 17 1832.0 18 1983.0 19 2029.0 20 2132.0 21 2340.5 22 2434.5 23 2517.0 24 2657.0 25 2794.5 26 3096.5 27 3395.0 28 3546.0 29 3884.5 30 4405.5 31 4835.5 32 5306.5 33 5990.5 34 6919.5 35 7619.5 36 8383.0 37 9500.5 38 10192.5 39 10670.5 40 11180.5 41 12016.0 42 12869.5 43 13944.0 44 15302.0 45 18414.0 46 33000.5 47 61544.5 48 84507.5 49 97783.0 50 98009.5 51 81691.5 52 63252.5 53 49240.5 54 40762.5 55 35746.5 56 31567.5 57 27609.5 58 24592.5 59 20600.5 60 17599.0 61 15514.5 62 12337.5 63 9463.0 64 7461.5 65 6510.0 66 5738.5 67 5038.5 68 4580.0 69 4089.0 70 3350.5 71 2419.0 72 1808.5 73 1495.5 74 1197.0 75 941.0 76 658.0 77 485.5 78 382.0 79 238.0 80 262.5 81 325.5 82 252.5 83 102.0 84 21.0 85 11.5 86 8.0 87 3.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2090263801137149 2 8.520097015504683E-4 3 0.0 4 0.11776667430319808 5 0.03294437512661811 6 0.0020826903815678115 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0011833468077089837 112-113 2.840032338501561E-4 114-115 7.573419569337496E-4 116-117 0.006248071144703434 118-119 0.002461361360034686 120-121 3.313371061585155E-4 122-123 0.002319359743109608 124-125 0.028352989512707254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1056326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.44025790415892 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42991582793887 36.18766460507556 2 10.009968392205582 8.8969115392419 3 2.8591626312008978 3.8118577416150448 4 1.431385719555624 2.544446021495281 5 0.8430849326416137 1.8733455920851876 6 0.5595647090104406 1.4920319989493773 7 0.40688653044915785 1.265749964562229 8 0.3062830001166575 1.0889036413475037 9 0.23221671439539862 0.9287793609649135 >10 1.6324929400064976 14.078642611614558 >50 0.15762071917363543 4.9043053027835155 >100 0.11544326218008874 10.155683727244872 >500 0.007880813088606092 2.341019933966048 >1k 0.00660284339856186 4.941585260902131 >5k 0.0012779696900442309 4.410567881412016 >10k+ 2.1299494834070516E-4 1.0785048167398168 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11394 1.0786442821628928 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9868 0.9341813038777801 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 9409 0.8907288090987062 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 9166 0.8677245471568436 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 7398 0.700351974674485 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5381 0.5094071337825633 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5374 0.5087444595702463 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3090 0.2925233308656608 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3060 0.28968329852715924 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3034 0.2872219371671246 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2818 0.26677370432991326 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2751 0.26043096544059313 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2264 0.21432777381225113 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2151 0.20363031867056194 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1891 0.17901670507021505 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1854 0.17551399851939647 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1798 0.17021260482086023 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1763 0.16689923375927507 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1645 0.15572843989450225 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1587 0.15023771070673259 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1572 0.1488176945374818 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1518 0.143705636328179 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1517 0.1436109685835623 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1490 0.14105493947891087 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1469 0.13906691684195976 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1320 0.1249614228940687 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1319 0.12486675514945197 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1203 0.1138852967739126 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1161 0.10990925150001041 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1152 0.10905724179845994 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1132 0.10716388690612556 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1130 0.10697455141689213 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1122 0.1062172094599584 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1119 0.10593320622610822 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1115 0.10555453524764134 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1082 0.10243049967528964 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1072 0.10148382222912244 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 9.46677446167187E-5 0.0 0.0 0.0 6 0.0 9.46677446167187E-5 0.0 0.0 0.0 7 0.0 9.46677446167187E-5 0.0 0.0 0.0 8 0.0 9.46677446167187E-5 0.0 0.0 0.0 9 0.0 9.46677446167187E-5 0.0 0.0 0.0 10-11 0.0 9.46677446167187E-5 0.0 0.0 0.0 12-13 0.0 9.46677446167187E-5 0.0 0.0 0.0 14-15 0.0 9.46677446167187E-5 0.0 0.0 0.0 16-17 0.0 9.46677446167187E-5 0.0 0.0 0.0 18-19 0.0 9.46677446167187E-5 0.0 0.0 0.0 20-21 0.0 9.46677446167187E-5 0.0 5.206725953919529E-4 0.0 22-23 0.0 9.46677446167187E-5 0.0 0.0023666936154179675 0.0 24-25 0.0 9.46677446167187E-5 0.0 0.004118046890827264 0.0 26-27 0.0 9.46677446167187E-5 0.0 0.006390072761628512 0.0 28-29 0.0 9.46677446167187E-5 0.0 0.011407463226314604 0.0 30-31 0.0 9.46677446167187E-5 0.0 0.03502706550818592 0.0 32-33 0.0 9.46677446167187E-5 0.0 0.08728366053661464 0.0 34-35 0.0 9.46677446167187E-5 0.0 0.14309029598817033 0.0 36-37 0.0 9.46677446167187E-5 0.0 0.2094997188367985 0.0 38-39 0.0 9.46677446167187E-5 0.0 0.3247576979076535 0.0 40-41 0.0 9.46677446167187E-5 0.0 0.49738433021624007 0.0 42-43 0.0 9.46677446167187E-5 0.0 0.8545657306551198 0.0 44-45 0.0 9.46677446167187E-5 0.0 1.3502460414682589 0.0 46-47 0.0 9.46677446167187E-5 0.0 1.8601265139739058 0.0 48-49 0.0 9.46677446167187E-5 0.0 2.0775309894862 0.0 50-51 0.0 9.46677446167187E-5 0.0 2.3514521085346756 0.0 52-53 0.0 9.46677446167187E-5 0.0 2.5760986665101493 0.0 54-55 0.0 9.46677446167187E-5 0.0 2.802733247122574 0.0 56-57 0.0 9.46677446167187E-5 0.0 3.1365790485134326 0.0 58-59 0.0 9.46677446167187E-5 0.0 3.519462741615751 0.0 60-61 0.0 9.46677446167187E-5 0.0 4.038194648243062 0.0 62-63 0.0 9.46677446167187E-5 0.0 4.677201924405912 0.0 64-65 0.0 9.46677446167187E-5 0.0 5.274934063915875 0.0 66-67 0.0 9.46677446167187E-5 0.0 5.778945136255285 0.0 68-69 0.0 1.893354892334374E-4 0.0 6.206133333838228 0.0 70-71 0.0 1.893354892334374E-4 0.0 6.708771723880696 0.0 72-73 0.0 1.893354892334374E-4 0.0 7.181258437262739 0.0 74-75 0.0 1.893354892334374E-4 0.0 7.669081325272691 0.0 76-77 0.0 1.893354892334374E-4 0.0 8.248495256199316 0.0 78-79 0.0 1.893354892334374E-4 0.0 8.995329093480612 0.0 80-81 0.0 3.786709784668748E-4 0.0 10.57178371071052 0.0 82-83 0.0 3.786709784668748E-4 0.0 11.725641515971395 0.0 84-85 0.0 3.786709784668748E-4 0.0 12.29497333209634 0.0 86-87 0.0 3.786709784668748E-4 0.0 12.788476284783297 0.0 88-89 0.0 3.786709784668748E-4 0.0 13.373617614259235 0.0 90-91 0.0 3.786709784668748E-4 0.0 14.060479435325837 0.0 92-93 0.0 3.786709784668748E-4 0.0 14.931612021288881 0.0 94-95 0.0 3.786709784668748E-4 0.0 16.084996487826675 0.0 96-97 0.0 3.786709784668748E-4 0.0 17.419906354667024 0.0 98-99 0.0 3.786709784668748E-4 0.0 18.74658012772572 0.0 100-101 0.0 3.786709784668748E-4 0.0 19.8970772280527 0.0 102-103 0.0 3.786709784668748E-4 0.0 20.924884931356416 0.0 104-105 0.0 3.786709784668748E-4 0.0 22.026912146439642 0.0 106-107 0.0 3.786709784668748E-4 0.0 23.07384273415593 0.0 108-109 0.0 3.786709784668748E-4 0.0 23.95666678657914 0.0 110-111 0.0 3.786709784668748E-4 0.0 24.921189102606583 0.0 112-113 0.0 3.786709784668748E-4 0.0 27.44720853221449 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 495 0.0 88.0455 1 AGTAGTA 1435 0.0 88.01286 2 GAGTAGT 1880 0.0 86.695045 1 AGTACGT 140 0.0 80.85144 2 GAGTCAG 4425 0.0 80.27738 1 GAGTTAG 1130 0.0 79.77889 1 GAGTAAG 940 0.0 74.310036 1 GAGTCGT 605 0.0 74.01085 1 GAGTCGA 355 0.0 73.99695 1 GTATACG 105 0.0 73.91687 1 TCGTAGG 940 0.0 72.25022 2 GAGTCCG 610 0.0 71.44676 1 AGTACGG 320 0.0 70.74501 2 AGTCCGT 265 0.0 69.69121 2 GAGTAAT 585 0.0 69.39729 1 GATTGTG 5965 0.0 68.422646 9 TACGCGG 150 0.0 67.51804 2 AGTTAGT 785 0.0 66.78443 2 AGTAAGG 420 0.0 66.66698 2 GAGTTAT 360 0.0 66.33565 1 >>END_MODULE