##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935932_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1116090 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96908761838203 31.0 31.0 34.0 28.0 34.0 2 31.05398668566155 33.0 31.0 34.0 28.0 34.0 3 31.31684183175192 34.0 31.0 34.0 28.0 34.0 4 34.31738211076168 37.0 35.0 37.0 32.0 37.0 5 34.57198971409116 37.0 35.0 37.0 32.0 37.0 6 34.798916754025214 37.0 35.0 37.0 32.0 37.0 7 34.781036475553044 37.0 35.0 37.0 32.0 37.0 8 34.728635683502226 37.0 35.0 37.0 32.0 37.0 9 36.351132077162234 39.0 38.0 39.0 32.0 39.0 10-11 36.10450456504404 39.0 37.0 39.0 31.5 39.0 12-13 35.90805759392164 39.0 37.0 39.0 31.0 39.0 14-15 37.42138358017723 40.0 38.0 41.0 31.5 41.0 16-17 37.607832701663845 40.5 38.0 41.0 32.0 41.0 18-19 37.65972681414581 41.0 38.0 41.0 32.0 41.0 20-21 37.79757815229954 41.0 38.0 41.0 32.0 41.0 22-23 37.71350742323648 40.5 38.0 41.0 32.0 41.0 24-25 37.6608002042846 40.0 38.0 41.0 32.0 41.0 26-27 37.635875243035954 40.0 38.0 41.0 31.5 41.0 28-29 37.527131324534764 40.0 38.0 41.0 31.0 41.0 30-31 37.442178050157246 40.0 37.0 41.0 31.0 41.0 32-33 37.36100717684057 40.0 37.0 41.0 30.5 41.0 34-35 37.09422268813447 40.0 37.0 41.0 30.0 41.0 36-37 37.090305441317454 40.0 36.5 41.0 30.0 41.0 38-39 37.223696565689146 40.0 37.0 41.0 30.5 41.0 40-41 37.195350733363796 40.0 36.0 41.0 30.0 41.0 42-43 37.00938096390077 40.0 36.0 41.0 30.0 41.0 44-45 36.798890770457575 40.0 35.0 41.0 30.0 41.0 46-47 36.62640423263357 40.0 35.0 41.0 30.0 41.0 48-49 36.45881828526373 40.0 35.0 41.0 30.0 41.0 50-51 36.21942764472399 39.0 35.0 41.0 30.0 41.0 52-53 35.9295737798923 39.0 35.0 41.0 30.0 41.0 54-55 35.61523757044682 38.5 35.0 41.0 29.5 41.0 56-57 35.29560564112214 37.5 35.0 40.0 29.0 41.0 58-59 34.88501375337114 37.0 35.0 39.5 28.5 41.0 60-61 34.54449820354989 36.5 34.5 39.0 28.0 41.0 62-63 34.061365122884354 36.0 34.0 39.0 27.5 41.0 64-65 33.55448754132731 35.0 34.0 37.5 26.5 40.0 66-67 33.20202895823813 35.0 34.0 37.0 26.5 39.0 68-69 32.990771353564675 35.0 34.0 36.0 27.0 39.0 70-71 32.86269700472184 35.0 34.0 36.0 28.0 38.0 72-73 32.64383696655288 35.0 34.0 35.5 27.5 37.0 74-75 32.37221684631167 35.0 34.0 35.0 27.0 37.0 76-77 32.242176706179606 35.0 34.0 35.0 27.0 36.0 78-79 32.1053467910294 35.0 33.5 35.0 26.5 36.0 80-81 31.96170290926359 35.0 33.0 35.0 26.0 35.5 82-83 31.894701144172963 35.0 33.0 35.0 26.5 35.0 84-85 31.687746507898108 35.0 33.0 35.0 25.0 35.0 86-87 31.52207931260024 35.0 33.0 35.0 25.0 35.0 88-89 31.36577919343422 35.0 33.0 35.0 24.5 35.0 90-91 31.287063767258914 35.0 33.0 35.0 24.0 35.0 92-93 31.21625585750253 35.0 33.0 35.0 23.0 36.0 94-95 31.06418657993531 35.0 33.0 35.0 21.5 35.0 96-97 30.960983881228216 35.0 33.0 35.0 20.5 35.0 98-99 30.796298237597327 35.0 33.0 35.0 19.5 35.0 100-101 30.62739250418873 35.0 32.0 35.0 18.5 35.0 102-103 29.94555143402414 34.0 30.5 35.0 17.0 35.0 104-105 30.469972403659202 35.0 32.0 35.0 18.0 35.0 106-107 30.47481520307502 35.0 32.0 35.0 16.5 35.0 108-109 30.348523864562893 35.0 32.0 35.0 14.0 35.0 110-111 30.257244935444277 35.0 32.0 35.0 9.0 35.0 112-113 30.174020464299474 35.0 32.0 35.0 4.5 35.0 114-115 29.965270721895187 35.0 31.0 35.0 2.0 35.0 116-117 29.74133716814952 35.0 31.0 35.0 2.0 35.0 118-119 29.456916108915948 35.0 31.0 35.0 2.0 35.0 120-121 29.135906602514133 35.0 31.0 35.0 2.0 35.0 122-123 28.574947360875914 35.0 30.0 35.0 2.0 35.0 124-125 25.664871112544688 32.0 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0919361827787526 1101 2 0.350812105118564 1101 3 0.2910678121838579 1101 4 0.12470345975276587 1101 5 0.16863448165062067 1101 6 0.2083920139740414 1101 7 0.1537231466271578 1101 8 0.28405159455819273 1101 9 0.24768596873452964 1101 10-11 0.20230081329565053 1101 12-13 0.17430524968733607 1101 14-15 0.16092826008434002 1101 16-17 0.2523973459369415 1101 18-19 0.252670299351081 1101 20-21 0.09324883815956753 1101 22-23 0.2010251551643023 1101 24-25 0.2233144262162412 1101 26-27 0.2126142989869777 1101 28-29 0.16510013446085736 1101 30-31 0.07880730681682735 1101 32-33 0.14280974070374697 1101 34-35 -0.009484169225984829 1101 36-37 -0.18617651892874676 1101 38-39 -0.06460240434817166 1101 40-41 -0.07325787727799593 1101 42-43 -0.12104965710781102 1101 44-45 0.06316872667323281 1101 46-47 -0.03893945016889688 1101 48-49 -0.08852280835198911 1101 50-51 -0.03526242269536084 1101 52-53 0.03272873186851655 1101 54-55 -0.12674516273284553 1101 56-57 -0.13413241787768726 1101 58-59 -0.12324762934112954 1101 60-61 -0.09819545660006668 1101 62-63 -0.0850465896389565 1101 64-65 -0.15862555492694952 1101 66-67 -0.1930372365794284 1101 68-69 -0.09998222195711293 1101 70-71 -0.2574335498544542 1101 72-73 -0.07282016696237292 1101 74-75 0.015471260604513759 1101 76-77 0.015013491608925733 1101 78-79 0.11259525451566077 1101 80-81 0.0010326442760408838 1101 82-83 -0.06299779351394719 1101 84-85 0.022020298167415575 1101 86-87 -0.21135415592326012 1101 88-89 -0.1321219901068389 1101 90-91 -0.22499737878344206 1101 92-93 -0.4494573864793594 1101 94-95 -0.4527004101613734 1101 96-97 -0.4399061322258788 1101 98-99 -0.6177293063193154 1101 100-101 -0.49948591940733067 1101 102-103 -0.3789416031649644 1101 104-105 -0.4220163388092253 1101 106-107 -0.2916412834562081 1101 108-109 -0.276656852549948 1101 110-111 -0.3082263744359892 1101 112-113 -0.26309415522454316 1101 114-115 -0.2771981454783692 1101 116-117 -0.3411703051055923 1101 118-119 -0.37475854274537923 1101 120-121 -0.464912268952709 1101 122-123 -0.36293245927475226 1101 124-125 -0.050140716739697666 1102 1 0.26522771574095927 1102 2 0.32282464874795735 1102 3 0.33911830332649373 1102 4 0.3849846491899811 1102 5 0.37270504858098263 1102 6 0.30587383331921814 1102 7 0.43929520235492703 1102 8 0.28113776450757655 1102 9 0.44473053766930093 1102 10-11 0.5000596355911497 1102 12-13 0.540706625599114 1102 14-15 0.5592570954362373 1102 16-17 0.6468849036715483 1102 18-19 0.605245714382761 1102 20-21 0.48041905886921654 1102 22-23 0.4962426216097384 1102 24-25 0.49612209452335065 1102 26-27 0.37025949686223925 1102 28-29 0.3575059264126921 1102 30-31 0.3021966197536443 1102 32-33 0.3298353253277355 1102 34-35 0.41827386590444604 1102 36-37 0.30693028969735536 1102 38-39 0.3120475090416832 1102 40-41 0.35327895259275977 1102 42-43 0.26723600729689423 1102 44-45 0.23574191653437282 1102 46-47 0.20869982417923438 1102 48-49 0.22021103002762743 1102 50-51 0.12057383465572258 1102 52-53 0.26850204239898545 1102 54-55 0.3955170969234487 1102 56-57 0.3144766966143777 1102 58-59 0.25670661833793673 1102 60-61 0.1922306371745961 1102 62-63 0.24337539064850233 1102 64-65 0.15321428732341502 1102 66-67 0.22072399206651738 1102 68-69 0.27290842850069197 1102 70-71 0.1979405668385894 1102 72-73 0.16867943967496757 1102 74-75 0.1882801602312938 1102 76-77 0.12242194628954906 1102 78-79 0.17531977796196685 1102 80-81 0.17813753028442036 1102 82-83 0.1970811076915524 1102 84-85 0.11349503654496829 1102 86-87 0.1942990525659809 1102 88-89 0.15144213504273196 1102 90-91 0.12930496208717912 1102 92-93 0.20703059078344666 1102 94-95 0.18064975120746496 1102 96-97 -0.033231588045030236 1102 98-99 0.03453788301323968 1102 100-101 -0.00408182008029101 1102 102-103 0.0620885188720095 1102 104-105 -0.0845958303885368 1102 106-107 -0.18383023121879205 1102 108-109 -0.11875527632884086 1102 110-111 0.05128466852255542 1102 112-113 0.023042108766880176 1102 114-115 -0.08864352567690048 1102 116-117 -0.008203435960133731 1102 118-119 -0.17423087829054396 1102 120-121 -0.3713106457215716 1102 122-123 -0.3203173649666624 1102 124-125 -0.5757873464638408 1103 1 0.20266296573788978 1103 2 0.2795031656971645 1103 3 0.2892755661864186 1103 4 0.2484849745541169 1103 5 0.37784567489741505 1103 6 0.22741133021270343 1103 7 0.40992606160837397 1103 8 0.3339204216889371 1103 9 0.267138029046194 1103 10-11 0.4523403386825109 1103 12-13 0.3959634050064693 1103 14-15 0.35419026348374416 1103 16-17 0.44690895140460185 1103 18-19 0.4699935158992119 1103 20-21 0.39987693490400034 1103 22-23 0.4571802969136911 1103 24-25 0.4390874763721371 1103 26-27 0.3375543682174609 1103 28-29 0.30596989863247614 1103 30-31 0.33732197491283245 1103 32-33 0.43541778640152273 1103 34-35 0.3256945390800894 1103 36-37 0.26788384158562906 1103 38-39 0.33163392190315477 1103 40-41 0.3818648485126488 1103 42-43 0.11436397553017486 1103 44-45 0.21980987000878116 1103 46-47 0.2835392907429366 1103 48-49 0.20849622767417486 1103 50-51 0.3227411789350114 1103 52-53 0.33879276170986117 1103 54-55 0.30727207761963626 1103 56-57 0.26436617441869714 1103 58-59 0.3629671154062777 1103 60-61 0.3252838228853321 1103 62-63 0.09009564819633198 1103 64-65 0.06393707835072604 1103 66-67 0.18394112150387087 1103 68-69 0.30746646038003433 1103 70-71 0.23288069518066123 1103 72-73 0.2644946260724126 1103 74-75 0.2934546989318463 1103 76-77 0.30415358628989964 1103 78-79 0.28022102772434465 1103 80-81 0.2072251262689555 1103 82-83 0.21393441948354308 1103 84-85 0.3889829874875872 1103 86-87 0.4333258983882615 1103 88-89 0.3154864133545985 1103 90-91 0.29072537022970835 1103 92-93 0.32217435431294206 1103 94-95 0.4106745737178379 1103 96-97 0.3661548983991203 1103 98-99 0.4288838714660912 1103 100-101 0.3999512133761165 1103 102-103 0.38772065036907577 1103 104-105 0.3350407101418398 1103 106-107 0.2306902387789087 1103 108-109 0.2350154547857528 1103 110-111 0.2940336207738312 1103 112-113 0.3216480599880853 1103 114-115 0.29520486522069334 1103 116-117 0.25008149495979026 1103 118-119 0.23287819045675917 1103 120-121 0.23945626104138285 1103 122-123 -0.010348013125039301 1103 124-125 -0.42577564012900027 1104 1 -0.06492186586556059 1104 2 0.0888747326266639 1104 3 0.19548154270289686 1104 4 0.06617223497502067 1104 5 0.13013641846648483 1104 6 0.16253000230001646 1104 7 0.2154123975310469 1104 8 0.1292235549633176 1104 9 0.24003316353527282 1104 10-11 0.30865743199382933 1104 12-13 0.1738650067609484 1104 14-15 0.2800805984742851 1104 16-17 0.34009656218646 1104 18-19 0.15896412861272324 1104 20-21 0.21744077732845568 1104 22-23 0.16132949628003246 1104 24-25 0.14589787628704443 1104 26-27 0.2822787360453063 1104 28-29 0.2755968920970062 1104 30-31 0.3118341287436195 1104 32-33 0.30064716118382506 1104 34-35 0.4097089042853028 1104 36-37 0.4027078532064081 1104 38-39 0.3264239773357005 1104 40-41 0.3447144076586923 1104 42-43 0.2176719432173968 1104 44-45 0.20070078555108495 1104 46-47 0.3618545051776465 1104 48-49 0.34291019924094 1104 50-51 0.28769371578381 1104 52-53 0.15679648373680521 1104 54-55 0.3084305422672813 1104 56-57 0.1457988300086015 1104 58-59 0.15768787000988738 1104 60-61 0.1882506697260382 1104 62-63 0.061285056658753945 1104 64-65 0.09750474348522431 1104 66-67 0.09078387804481736 1104 68-69 0.2089458118184595 1104 70-71 0.21593386154244598 1104 72-73 0.002978996238717002 1104 74-75 0.06579579217170561 1104 76-77 0.10045055921995072 1104 78-79 0.07084808046199242 1104 80-81 0.10392466921639354 1104 82-83 0.044594372860771614 1104 84-85 -0.030772376932461043 1104 86-87 0.0312256001551674 1104 88-89 0.0462683231993779 1104 90-91 0.09685477887075322 1104 92-93 0.1878005747910727 1104 94-95 0.14528568929473096 1104 96-97 0.03041268124352925 1104 98-99 0.08644663398306918 1104 100-101 0.2080335900169885 1104 102-103 0.1488552677367494 1104 104-105 0.15634626955420927 1104 106-107 0.14627476508520942 1104 108-109 0.20624261941155453 1104 110-111 0.13788245159130952 1104 112-113 0.09242288141766863 1104 114-115 0.14266773223699047 1104 116-117 0.07483585660862246 1104 118-119 0.0802961966552651 1104 120-121 0.13611926576618316 1104 122-123 0.1392345985783976 1104 124-125 0.5235637082077389 1105 1 5.198218714781433E-4 1105 2 0.04216092099581559 1105 3 0.11711476014916755 1105 4 0.2348598139889262 1105 5 0.037178927948154694 1105 6 0.1379708998979936 1105 7 0.1979763987952694 1105 8 0.13820269529201568 1105 9 0.17194213951504622 1105 10-11 0.3363360034223959 1105 12-13 0.3359517640302272 1105 14-15 0.30171816168541454 1105 16-17 0.3778209616807757 1105 18-19 0.27492888841045016 1105 20-21 0.1834418835231375 1105 22-23 0.2535541328160633 1105 24-25 0.33229958298742446 1105 26-27 0.3875248643638969 1105 28-29 0.23052854506793352 1105 30-31 0.30226356932516296 1105 32-33 0.37619512262503463 1105 34-35 0.4249360692663444 1105 36-37 0.31673479694850215 1105 38-39 0.2697611296745137 1105 40-41 0.2809849512743696 1105 42-43 0.2870046707774492 1105 44-45 0.2643370055258103 1105 46-47 0.30432606017132713 1105 48-49 0.11858734841919727 1105 50-51 0.15025021072691658 1105 52-53 0.11113079157498618 1105 54-55 0.26651722052265825 1105 56-57 0.2782996201476635 1105 58-59 0.1550839667229127 1105 60-61 0.11538839096497355 1105 62-63 0.10102391569794378 1105 64-65 0.09403642806170609 1105 66-67 0.10145075541523596 1105 68-69 0.2777673194038002 1105 70-71 0.24548506255381852 1105 72-73 0.16391057019573196 1105 74-75 0.08157407915020798 1105 76-77 0.1410135333033864 1105 78-79 0.19047592496262666 1105 80-81 0.1006847039825054 1105 82-83 0.19153376919452825 1105 84-85 0.2384260269866303 1105 86-87 0.17779402619815343 1105 88-89 0.2315531683321197 1105 90-91 0.3038443490351028 1105 92-93 0.3330640861690739 1105 94-95 0.27811596742368394 1105 96-97 0.2000748967934669 1105 98-99 0.21070548354059326 1105 100-101 0.2866540072104158 1105 102-103 0.13763173739540946 1105 104-105 0.2785065097564825 1105 106-107 0.17981656344172237 1105 108-109 0.23520793230662562 1105 110-111 0.23122513490357122 1105 112-113 0.31284126005230917 1105 114-115 0.3632800900119584 1105 116-117 0.1842875000978772 1105 118-119 0.11436462269825043 1105 120-121 0.11466620002661188 1105 122-123 0.2664896554684084 1105 124-125 0.23748082261987236 1106 1 -0.13330811211385196 1106 2 -0.0353467195909829 1106 3 0.05663034694623548 1106 4 -0.7842554060599696 1106 5 0.06033449029533955 1106 6 0.09842016640165951 1106 7 0.08540457727937678 1106 8 0.006423966685659366 1106 9 0.03597636934389925 1106 10-11 0.22177304498719508 1106 12-13 0.21146673958035223 1106 14-15 0.19869936584188963 1106 16-17 0.3954615484778401 1106 18-19 0.32255266591656095 1106 20-21 0.35882544098035396 1106 22-23 0.41235532474760817 1106 24-25 0.4018442040143242 1106 26-27 0.3487099193761125 1106 28-29 0.15528633846646045 1106 30-31 0.25258065367724214 1106 32-33 0.3279272436519278 1106 34-35 0.2945441071636523 1106 36-37 0.29563531650840247 1106 38-39 0.2732566981341691 1106 40-41 0.26898006129881935 1106 42-43 0.19095867444492853 1106 44-45 0.2064794260637015 1106 46-47 0.25388596237181815 1106 48-49 0.19697518460500874 1106 50-51 0.14268832197386416 1106 52-53 0.2792321057559164 1106 54-55 0.3122912119651957 1106 56-57 0.33956642943861937 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48-49 -0.5985029504663189 2216 50-51 -0.68896299849294 2216 52-53 -0.5650745655678762 2216 54-55 -0.7103422652929439 2216 56-57 -0.5533879431755366 2216 58-59 -0.7206800277046455 2216 60-61 -0.7075114950532608 2216 62-63 -0.792896740330427 2216 64-65 -0.6210398763962459 2216 66-67 -0.6943319193567987 2216 68-69 -0.9111754997451413 2216 70-71 -0.7207003477805287 2216 72-73 -0.7708507013341581 2216 74-75 -0.7514635348315508 2216 76-77 -0.8089804509924576 2216 78-79 -1.0673489230616902 2216 80-81 -0.8401850401917557 2216 82-83 -1.0143556051722413 2216 84-85 -0.9423664628816582 2216 86-87 -1.063232442900123 2216 88-89 -0.9031672597327258 2216 90-91 -1.0595434422516234 2216 92-93 -0.8177360024834073 2216 94-95 -0.7652414287262665 2216 96-97 -0.7936855515348 2216 98-99 -0.8020440098737929 2216 100-101 -0.9009181812394331 2216 102-103 -0.8554619176197882 2216 104-105 -1.073027178186777 2216 106-107 -0.8930864887365964 2216 108-109 -1.1575127490510226 2216 110-111 -1.3117817673254066 2216 112-113 -1.4681589299172941 2216 114-115 -1.3598069160670683 2216 116-117 -1.3577299771057696 2216 118-119 -1.4199369223878726 2216 120-121 -1.1607219711993224 2216 122-123 -0.8859153597291609 2216 124-125 -0.7819970775827585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16882.0 3 2911.0 4 2011.0 5 1613.0 6 2017.0 7 2520.0 8 3504.0 9 3619.0 10 4355.0 11 4644.0 12 3989.0 13 3374.0 14 3139.0 15 3041.0 16 3097.0 17 3021.0 18 2823.0 19 2705.0 20 2959.0 21 3167.0 22 4001.0 23 5580.0 24 8077.0 25 11680.0 26 13287.0 27 11977.0 28 13692.0 29 17123.0 30 19933.0 31 25991.0 32 35658.0 33 49057.0 34 79200.0 35 116045.0 36 247161.0 37 346889.0 38 35235.0 39 113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.63814349444982 8.444323589883037 5.227019609849168 10.69051330581798 2 10.005376777697126 36.2872453871728 42.96618902958124 10.741188805548834 3 43.15401626505808 19.03268065217937 16.28244140744006 21.530861675322488 4 14.528483814425968 15.507217285641062 50.63606026173638 19.328238638196595 5 16.990053339760745 32.38959081692357 26.82533893999658 23.79501690331911 6 30.150256699728516 39.7096112320691 14.072162639213683 16.0679694289887 7 47.464404720412084 21.366217210276243 15.438732932588708 15.730645136722968 8 44.90921390395893 21.686878687555158 15.244717024240986 18.159190384244926 9 62.55660385811181 9.362685804908207 8.365275201820642 19.71543513515935 10-11 43.48238045318926 25.355930077323514 16.681674416937703 14.480015052549525 12-13 29.17367775000224 30.73654454389879 22.943669417340896 17.146108288758075 14-15 25.072977985646318 31.114560653710722 20.998485785196536 22.813975575446424 16-17 23.0706305047084 35.750521911315396 20.39521902355545 20.783628560420755 18-19 23.109785053176715 26.612056375381915 25.40319329086364 24.874965280577733 20-21 23.182807838077572 28.791943302063455 21.72925122525961 26.29599763459936 22-23 25.22892419070147 23.743336110887114 22.00042111299268 29.027318585418737 24-25 22.478384359684256 22.46781173561272 34.04729905294376 21.006504851759267 26-27 17.51824130807886 22.134220725315714 32.96767837774997 27.379859588855453 28-29 21.47225108277475 23.997344082451725 23.057647478916863 31.47275735585666 30-31 21.611877178363752 28.703419975091617 20.729466261681406 28.955236584863226 32-33 30.16526444999955 25.536291876103185 18.273660726285517 26.02478294761175 34-35 29.06942988468672 18.46284797820964 24.924020464299474 27.543701672804165 36-37 24.88697148079456 25.7773566647851 28.667714969222914 20.66795688519743 38-39 22.370418156241882 30.54637170837477 24.0562141045973 23.026996030786048 40-41 20.43656873549624 30.87358546353789 25.16405487012696 23.525790930838912 42-43 21.176697219758264 22.867869078658533 22.904918062163446 33.05051563941976 44-45 23.311560895626695 23.717755736544543 22.061079303640387 30.909604064188372 46-47 25.926762178677347 21.639787113942425 22.912623533944394 29.520827173435833 48-49 17.424132462435825 22.365445438987898 26.21795733318998 33.9924647653863 50-51 22.913698716053364 24.71946706806799 24.56809038697596 27.79874382890269 52-53 26.028635683502227 21.09870171760342 28.129317528156335 24.743345070738023 54-55 21.041761865082563 23.388122821636248 29.219776182924317 26.350339130356872 56-57 20.05339208643085 25.981893819724878 26.421422062696053 27.54329203114822 58-59 22.46915571324893 23.195217231585268 31.862663405280937 22.472963649884868 60-61 24.460851929885553 29.449833548295185 28.008327289164537 18.08098723265473 62-63 26.249585606895504 25.789049270220143 25.931242104131385 22.030123018752967 64-65 31.419741969358526 18.9750364896794 28.701585654650387 20.90363588631169 66-67 31.46061828402574 23.66706418574146 23.599686046542885 21.27263148368992 68-69 23.218305113905178 28.786236039626672 24.888756075181718 23.106702771286432 70-71 24.203446638049236 29.153709661519713 21.77690949662595 24.865934203805104 72-73 22.802144633756477 28.990769545053794 22.41176323013956 25.79532259105017 74-75 20.802516151578416 29.840052330218015 25.713133630229663 23.644297887973906 76-77 22.75354352332326 28.73050496579339 22.622281178278904 25.893670332604447 78-79 23.535125056391742 30.99957753791967 18.916176444894234 26.549120960794355 80-81 21.887826735968936 23.434673414110314 24.877271012663105 29.80022883725764 82-83 24.409915154653817 18.670411288700414 28.508690387285252 28.41098316936052 84-85 25.603463842802547 19.97238926918491 32.55857198483157 21.86557490318097 86-87 28.53529380140853 22.41469992652725 24.37359998566385 24.676406286400372 88-89 23.53382926346221 25.726711492154642 26.397301509208404 24.342157735174744 90-91 19.843041191159486 28.645744201403296 25.687915569457275 25.823299037979947 92-93 23.42989075961964 23.536527938268723 26.487241399241086 26.546339902870557 94-95 22.153550853403804 22.691705579944497 27.965720471386085 27.18902309526562 96-97 22.434951999154137 21.283809817369193 27.8154910924695 28.465747091007167 98-99 22.246135732967524 23.145595349349808 29.702103562625275 24.906165355057393 100-101 20.42013117339259 27.802039280338324 26.829304350942586 24.9485251953265 102-103 20.768486412386096 27.265856696144574 26.517261152774417 25.448395738694906 104-105 20.735948519980806 24.412913549927044 26.198532636671 28.652605293421146 106-107 20.33709083317579 26.25875946057582 23.13639984266347 30.267749863584925 108-109 22.790948758612657 26.905849886657883 24.8113951383849 25.49180621634456 110-111 23.960254101371753 28.07049610694478 22.64373840819289 25.32551138349058 112-113 21.09772842835735 29.324128476741084 23.981891938507854 25.596251156393706 114-115 23.07590297157721 25.540636017073115 25.923154843961623 25.46030616738805 116-117 27.07590785689326 24.62265587900617 23.78056429141019 24.520871972690376 118-119 23.49107929441355 25.875224446701058 25.470917760038738 25.16277849884666 120-121 20.324289404527132 26.6108518185671 26.592215011410563 26.472643765495206 122-123 21.63626205891142 27.36463979735339 27.704101541936765 23.294996601798427 124-125 22.28552566281861 29.344737533486175 22.861499147828702 25.508237655866512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 35.0 2 55.5 3 59.5 4 52.5 5 50.5 6 50.0 7 51.0 8 56.0 9 55.5 10 69.0 11 90.5 12 109.5 13 144.0 14 187.5 15 237.5 16 293.5 17 343.5 18 433.0 19 515.5 20 580.5 21 724.0 22 905.5 23 1106.0 24 1303.0 25 1570.5 26 1868.0 27 2116.0 28 2408.0 29 2781.0 30 3274.0 31 3661.5 32 4172.0 33 4789.0 34 5443.0 35 5901.5 36 6369.5 37 7279.0 38 7851.0 39 7998.5 40 8114.0 41 8466.5 42 8915.5 43 9547.5 44 10642.5 45 14604.5 46 30804.5 47 63968.0 48 95419.0 49 122757.5 50 133092.0 51 111716.0 52 82060.0 53 59152.5 54 45302.0 55 39758.5 56 36650.5 57 31153.5 58 25226.5 59 19497.0 60 16154.5 61 13873.0 62 10811.5 63 7929.0 64 5737.0 65 4813.0 66 4337.0 67 3752.0 68 3323.0 69 2801.5 70 2173.5 71 1639.5 72 1352.5 73 1224.0 74 1014.5 75 821.0 76 568.5 77 413.0 78 353.0 79 269.0 80 260.5 81 252.0 82 179.0 83 81.5 84 29.5 85 23.0 86 16.0 87 11.0 88 6.5 89 6.0 90 7.0 91 3.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16154611187269843 2 0.016127731634545603 3 0.006988683708303094 4 0.014156564434767805 5 0.003315144835989929 6 0.0 7 8.959850908080889E-5 8 4.4799254540404444E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.407843453484934E-4 28-29 0.008198263580894014 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 6.271895635656622E-4 58-59 0.0 60-61 4.479925454040444E-5 62-63 0.0 64-65 0.0018815686906969867 66-67 8.959850908080889E-5 68-69 0.006630289671979858 70-71 0.019711671997777958 72-73 1.7919701816161777E-4 74-75 0.00895985090808089 76-77 4.0319329086364E-4 78-79 0.00120957987259092 80-81 0.0011647806180505155 82-83 4.479925454040444E-5 84-85 0.019666872743237553 86-87 0.002687955272424267 88-89 0.01854689137972744 90-91 8.959850908080889E-5 92-93 0.014022166671146592 94-95 0.013170980834878906 96-97 0.006092698617495005 98-99 0.002777553781505076 100-101 8.959850908080889E-4 102-103 0.0 104-105 5.823903090252578E-4 106-107 9.855835998888978E-4 108-109 0.0 110-111 0.0 112-113 0.0011199813635101113 114-115 0.005958300853873792 116-117 0.0 118-119 0.07593473644598554 120-121 0.001702371672535369 122-123 0.00524151278122732 124-125 0.2470678887903305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1116090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.571431015854685 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20804773897157 27.729069893242357 2 9.835376413042212 6.800460743569063 3 3.1289198962310145 3.2451371504005717 4 1.5097731143766058 2.0878006829305167 5 0.9354014827723639 1.6169083916896483 6 0.6290909761395365 1.3049145170582805 7 0.4760772427086556 1.1521070088164416 8 0.3446921756779921 0.9533201418525256 9 0.25847505594901765 0.804226730945458 >10 2.1748928663371503 15.13583189606714 >50 0.2803601139600105 6.766330249443681 >100 0.1880300589810629 12.711419020852535 >500 0.016857867356557373 3.971472262332281 >1k 0.01193018305233291 8.090348245242389 >5k 0.001037407221941992 2.705270716103526 >10k+ 0.001037407221941992 4.925382349453632 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 16172 1.4489870888548413 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14039 1.257873468985476 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12672 1.1353923070720104 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 12050 1.079662034423747 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9918 0.8886380130634626 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 9167 0.8213495327437752 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5714 0.511965880887742 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 5373 0.4814127892911862 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 4756 0.42613050918832707 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4405 0.3946814325009632 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 4072 0.36484512897705385 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4040 0.3619779766864679 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3628 0.32506339094517467 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 3155 0.2826832961499521 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3133 0.2807121289501743 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2922 0.2618068435341236 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2910 0.26073166142515386 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2787 0.24971104480821438 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2739 0.24541031637233557 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2430 0.21772437706636563 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2307 0.20670376044942615 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2031 0.18197457194312286 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1997 0.17892822263437538 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1942 0.17400030463493088 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1871 0.16763881049019344 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1870 0.16754921198111264 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1814 0.16253169547258733 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1765 0.1581413685276277 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1757 0.15742458045498123 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1701 0.15240706394645595 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1616 0.14479119067458718 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1575 0.141117651802274 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1564 0.1401320682023851 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1531 0.13717531740271843 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1454 0.13027623220349613 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1431 0.12821546649463753 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1411 0.12642349631302136 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1399 0.12534831420405165 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1389 0.12445232911324357 No Hit GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1309 0.11728444838677884 No Hit GAGTCCGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1218 0.10913098406042523 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1202 0.1076974079151323 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1182 0.10590543773351611 No Hit GAGTCGGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1166 0.10447186158822318 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1117 0.10008153464326354 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.95985090808089E-5 0.0 0.0 0.0 0.0 7 8.95985090808089E-5 0.0 0.0 0.0 0.0 8 8.95985090808089E-5 0.0 0.0 0.0 0.0 9 8.95985090808089E-5 0.0 0.0 0.0 0.0 10-11 8.95985090808089E-5 0.0 0.0 0.0 0.0 12-13 8.95985090808089E-5 0.0 0.0 0.0 0.0 14-15 1.791970181616178E-4 0.0 0.0 0.0 0.0 16-17 1.791970181616178E-4 0.0 0.0 0.0 0.0 18-19 1.791970181616178E-4 0.0 0.0 4.479925454040445E-5 0.0 20-21 1.791970181616178E-4 0.0 0.0 5.375910544848534E-4 0.0 22-23 1.791970181616178E-4 0.0 0.0 0.002105564963399009 0.0 24-25 1.791970181616178E-4 0.0 0.0 0.004345527690419232 0.0 26-27 1.791970181616178E-4 0.0 0.0 0.006495891908358645 0.0 28-29 2.687955272424267E-4 0.0 0.0 0.013350177853040525 0.0 30-31 2.687955272424267E-4 0.0 0.0 0.048786388194500444 0.0 32-33 2.687955272424267E-4 0.0 0.0 0.11974840738650108 0.0 34-35 2.687955272424267E-4 0.0 0.0 0.20213423648630485 0.0 36-37 2.687955272424267E-4 0.0 0.0 0.3023053696386492 0.0 38-39 2.687955272424267E-4 0.0 0.0 0.45587721420315563 0.0 40-41 2.687955272424267E-4 0.0 0.0 0.7029003037389459 0.0 42-43 2.687955272424267E-4 0.0 0.0 1.3131109498337947 0.0 44-45 2.687955272424267E-4 0.0 0.0 2.2028689442607674 0.0 46-47 3.135947817828311E-4 0.0 0.0 3.105215529213594 0.0 48-49 3.583940363232356E-4 0.0 0.0 3.398426650180541 0.0 50-51 3.583940363232356E-4 0.0 0.0 3.7880457669184384 0.0 52-53 3.583940363232356E-4 0.0 0.0 4.085557616321264 0.0 54-55 3.583940363232356E-4 0.0 0.0 4.3793511275972365 0.0 56-57 3.583940363232356E-4 0.0 0.0 4.852924047343852 0.0 58-59 3.583940363232356E-4 0.0 0.0 5.3960702093917154 0.0 60-61 3.583940363232356E-4 0.0 0.0 6.223512440752986 0.0 62-63 3.583940363232356E-4 0.0 0.0 7.2939458287414105 0.0 64-65 3.583940363232356E-4 0.0 0.0 8.25650261179654 0.0 66-67 3.583940363232356E-4 0.0 0.0 9.006173337275667 0.0 68-69 3.583940363232356E-4 0.0 0.0 9.577811825211228 0.0 70-71 3.583940363232356E-4 0.0 0.0 10.250069438844537 0.0 72-73 3.583940363232356E-4 0.0 0.0 10.907453699970432 0.0 74-75 3.583940363232356E-4 0.0 0.0 11.65027013950488 0.0 76-77 3.583940363232356E-4 0.0 0.0 12.566683690383393 0.0 78-79 3.583940363232356E-4 0.0 0.0 13.72071248734421 0.0 80-81 3.583940363232356E-4 0.0 0.0 16.109050345402252 0.0 82-83 3.583940363232356E-4 0.0 0.0 17.798161438593663 0.0 84-85 3.583940363232356E-4 0.0 0.0 18.567319839797864 0.0 86-87 3.583940363232356E-4 0.0 0.0 19.214041878343146 0.0 88-89 3.583940363232356E-4 0.0 0.0 19.94883925131486 0.0 90-91 3.583940363232356E-4 0.0 0.0 20.88415808760942 0.0 92-93 3.583940363232356E-4 0.0 0.0 22.24542823607415 0.0 94-95 3.583940363232356E-4 0.0 0.0 24.228825632341476 0.0 96-97 3.583940363232356E-4 0.0 0.0 26.562553199114767 0.0 98-99 3.583940363232356E-4 0.0 0.0 28.764122964993863 0.0 100-101 3.583940363232356E-4 0.0 0.0 30.511473089087797 0.0 102-103 4.0319329086364007E-4 0.0 0.0 31.879911118278994 0.0 104-105 5.375910544848534E-4 0.0 0.0 33.38471807829117 0.0 106-107 5.375910544848534E-4 0.0 0.0 34.822370955747296 0.0 108-109 5.375910544848534E-4 0.0 0.0 35.94710104023869 0.0 110-111 5.375910544848534E-4 0.0 0.0 37.04571315933303 0.0 112-113 5.375910544848534E-4 0.0 0.0 40.17449309643487 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 2585 0.0 94.282814 1 GAGTCCG 905 0.0 93.499565 1 AGTAGTA 2185 0.0 93.40074 2 GAGTAAT 535 0.0 92.44727 1 GAGTAAG 1200 0.0 91.867165 1 GAGTTAC 765 0.0 90.35794 1 GAGTCGA 370 0.0 88.578995 1 GAGTAGC 810 0.0 88.280754 1 GAGTTAG 1215 0.0 88.280754 1 GAGTCGG 865 0.0 88.17869 1 GAGTCAG 6155 0.0 88.10146 1 GAGTACG 755 0.0 86.819145 1 GAGTAGG 1280 0.0 86.591 1 AGTCCGT 420 0.0 84.99818 2 AGTTTCG 140 0.0 84.99818 2 GAGTCGT 900 0.0 84.08742 1 GAGTTAA 360 0.0 82.76321 1 AGTCGTA 580 0.0 82.06721 2 AGTCGGT 410 0.0 79.81536 2 AGTCAGA 1300 0.0 79.63675 2 >>END_MODULE