FastQCFastQC Report
Thu 26 May 2016
SRR935931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401397
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8640.21524824550258223No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT8130.20254261989999925No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7380.183857876366789No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6830.17015573110910148No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC6110.1522183773172196No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4450.11086281163038089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTATG152.6992543E-4119.012832
ACCTAAG250.002039791371.541441
TTCTAGG359.14029E-568.007332
GTTCTAG401.7533413E-459.617871
CTAGGCA300.004236916559.491594
TGTGCAA150.0042405559.48417710-11
CTTAATC350.00779693350.992794
TTAATCA350.00780077450.986445
TAACAAC1450.049.338931
TGGAAAC856.182745E-849.0052872
TAGAACA505.332144E-447.5932734
CTATGGG751.469838E-647.593273
GTACCGA255.3386315E-447.5873436-37
TATAAGG1056.361006E-945.3382262
TCTATGG802.2884415E-644.6298142
GGAAACA3750.044.514671
ACAACAC2700.044.0623555
TTAGGGC558.527702E-443.266614
GTTATAG1256.0936145E-1042.9248661
TTATAGG1256.202754E-1042.8446242
Produced by FastQC (version 0.11.5)