##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935931_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401397 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.357212435568776 31.0 31.0 34.0 16.0 34.0 2 29.231080451523056 31.0 31.0 34.0 16.0 34.0 3 29.267525666609366 33.0 31.0 34.0 16.0 34.0 4 32.41718298841297 37.0 35.0 37.0 17.0 37.0 5 32.56250046711859 37.0 35.0 37.0 17.0 37.0 6 32.734689098324104 37.0 35.0 37.0 18.0 37.0 7 32.70175910632118 37.0 35.0 37.0 18.0 37.0 8 32.48514313759196 37.0 35.0 37.0 15.0 37.0 9 33.77896197530126 39.0 35.0 39.0 10.0 39.0 10-11 33.807960697264804 39.0 35.0 39.0 13.5 39.0 12-13 33.80016542226275 39.0 35.0 39.0 14.0 39.0 14-15 34.863177851354145 40.0 36.0 41.0 16.0 41.0 16-17 34.94442783578353 40.0 36.0 41.0 18.0 41.0 18-19 35.09128120040758 40.0 36.0 41.0 20.5 41.0 20-21 35.236249897233904 40.0 36.0 41.0 23.0 41.0 22-23 35.01771687381819 40.0 35.0 41.0 22.0 41.0 24-25 34.753363876660764 40.0 35.0 41.0 20.5 41.0 26-27 34.58887211414137 40.0 35.0 41.0 17.0 41.0 28-29 34.40165970348558 40.0 35.0 41.0 10.5 41.0 30-31 34.209596733408574 40.0 35.0 41.0 2.0 41.0 32-33 33.90209069823641 40.0 34.0 41.0 2.0 41.0 34-35 33.65314264929733 40.0 34.0 41.0 2.0 41.0 36-37 33.514970216518805 39.5 34.0 41.0 2.0 41.0 38-39 33.38095825330035 39.0 34.0 41.0 2.0 41.0 40-41 33.16052312299295 39.0 34.0 41.0 2.0 41.0 42-43 32.90404387676041 39.0 34.0 41.0 2.0 41.0 44-45 32.569555826276726 38.5 33.0 41.0 2.0 41.0 46-47 32.21412591524102 37.5 33.0 40.5 2.0 41.0 48-49 31.856067683614974 37.0 33.0 40.0 2.0 41.0 50-51 31.474256160359943 36.5 33.0 39.5 2.0 41.0 52-53 31.126844495599123 36.0 32.5 39.0 2.0 41.0 54-55 30.738713792081157 35.0 32.0 39.0 2.0 41.0 56-57 30.38990326285448 35.0 32.0 37.0 2.0 40.5 58-59 30.0436388413466 35.0 31.5 37.0 2.0 39.5 60-61 29.74022351935864 35.0 31.0 36.0 2.0 39.0 62-63 29.42965941449488 35.0 31.0 36.0 2.0 39.0 64-65 29.207577535457414 35.0 31.0 35.0 2.0 37.0 66-67 29.052668555071413 35.0 31.0 35.0 2.0 37.0 68-69 28.94247714855866 35.0 31.0 35.0 2.0 36.0 70-71 28.782206892428192 35.0 31.0 35.0 2.0 36.0 72-73 28.63116316265443 35.0 31.0 35.0 2.0 35.0 74-75 28.44315478192413 35.0 31.0 35.0 2.0 35.0 76-77 28.237844328682076 35.0 30.0 35.0 2.0 35.0 78-79 28.02696333056799 35.0 29.5 35.0 2.0 35.0 80-81 27.771398391119014 35.0 29.0 35.0 2.0 35.0 82-83 27.5061522632207 35.0 29.0 35.0 2.0 35.0 84-85 27.238056089108788 35.0 27.0 35.0 2.0 35.0 86-87 26.97689693744597 34.0 25.5 35.0 2.0 35.0 88-89 26.644573825913 34.0 25.0 35.0 2.0 35.0 90-91 26.27675717556434 34.0 23.5 35.0 2.0 35.0 92-93 25.86576755680785 34.0 19.5 35.0 2.0 35.0 94-95 25.485125698497995 34.0 18.0 35.0 2.0 35.0 96-97 25.120205183396987 33.0 15.0 35.0 2.0 35.0 98-99 24.71414584563412 33.0 9.0 35.0 2.0 35.0 100-101 24.391959830292702 33.0 7.0 35.0 2.0 35.0 102-103 23.651297842285814 31.5 5.0 34.5 2.0 35.0 104-105 24.01000381168768 32.0 2.0 35.0 2.0 35.0 106-107 23.97113456254033 33.0 2.0 35.0 2.0 35.0 108-109 23.73221274698117 32.0 2.0 35.0 2.0 35.0 110-111 23.331402576501567 32.0 2.0 35.0 2.0 35.0 112-113 22.841378983898736 32.0 2.0 35.0 2.0 35.0 114-115 22.2348933848534 31.0 2.0 35.0 2.0 35.0 116-117 21.531630779502585 31.0 2.0 35.0 2.0 35.0 118-119 20.60599107616649 29.5 2.0 35.0 2.0 35.0 120-121 19.610586277426087 29.0 2.0 35.0 2.0 35.0 122-123 18.62664270036896 26.0 2.0 34.5 2.0 35.0 124-125 16.17870462410033 12.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33650430345585036 1101 2 0.46650316114089563 1101 3 0.46209611723396193 1101 4 0.34501242914290486 1101 5 0.32986519400671455 1101 6 0.3019347848479086 1101 7 0.3214342508653374 1101 8 -0.07550469686853489 1101 9 0.01775515146869111 1101 10-11 0.3249764548922087 1101 12-13 0.009465357267011143 1101 14-15 0.2531479593806907 1101 16-17 0.33953682041668287 1101 18-19 0.38104046237015154 1101 20-21 0.243785639940981 1101 22-23 0.40077079672272475 1101 24-25 0.3035071280839432 1101 26-27 0.11701176632170274 1101 28-29 0.1306638870788035 1101 30-31 0.07912234679650965 1101 32-33 -0.07209088525638663 1101 34-35 0.09317947576474239 1101 36-37 0.04474911229499412 1101 38-39 0.016996929354469614 1101 40-41 0.19021212212652472 1101 42-43 0.19770941216359006 1101 44-45 0.2580715773913127 1101 46-47 0.18161127458199644 1101 48-49 0.2464765212454374 1101 50-51 0.3561416470270622 1101 52-53 0.4179505110356061 1101 54-55 0.1619423984212638 1101 56-57 0.14173982648630457 1101 58-59 -0.030125635816062868 1101 60-61 -0.04292882287516164 1101 62-63 0.17922231200529737 1101 64-65 0.04920342407843137 1101 66-67 -0.05227401556684441 1101 68-69 -0.0020177680510791163 1101 70-71 -0.23073883364776293 1101 72-73 -0.16174579234987263 1101 74-75 0.054812644476520234 1101 76-77 0.1564965451969691 1101 78-79 0.11557583797347348 1101 80-81 0.09193038591688563 1101 82-83 0.1712910996583119 1101 84-85 -0.08907210988081715 1101 86-87 -0.04847734819907501 1101 88-89 0.23351396789370682 1101 90-91 -0.00999050122485201 1101 92-93 -0.3166182642208959 1101 94-95 -0.04168263350361201 1101 96-97 0.26315048595359514 1101 98-99 -0.23741495232971488 1101 100-101 0.04742117124087386 1101 102-103 0.22719701707300644 1101 104-105 -0.18994213194314824 1101 106-107 0.21714142915189427 1101 108-109 0.29058465207932116 1101 110-111 0.3525580717870511 1101 112-113 0.3463510644598884 1101 114-115 0.20315353046246187 1101 116-117 -0.3160691591601825 1101 118-119 -0.13066115090861175 1101 120-121 -0.45169489681914854 1101 122-123 -0.5350281695229349 1101 124-125 -0.2257023376129652 1102 1 0.07561909164042291 1102 2 -0.035663339524319326 1102 3 0.09649549491027898 1102 4 0.1979666490936154 1102 5 -0.18298477218714737 1102 6 -0.004683220372044161 1102 7 0.22988505748697463 1102 8 0.02656074889988247 1102 9 0.16128945560758012 1102 10-11 0.09486391317979326 1102 12-13 0.27749711322306325 1102 14-15 0.30355726806340755 1102 16-17 0.20260925278623887 1102 18-19 0.15379417195727285 1102 20-21 0.35578390964850115 1102 22-23 0.540755456734793 1102 24-25 0.3495289094127898 1102 26-27 0.22971160419765368 1102 28-29 0.12279214682624229 1102 30-31 -0.15272048739348776 1102 32-33 0.02294797362299761 1102 34-35 0.1658681176305521 1102 36-37 -0.027935894502576275 1102 38-39 -0.00517335178170697 1102 40-41 -0.11022444956792299 1102 42-43 -0.026241688898579696 1102 44-45 0.015783374641742398 1102 46-47 0.011201093482895885 1102 48-49 0.22704526402074166 1102 50-51 0.03596120224011656 1102 52-53 0.0680261204547925 1102 54-55 0.29657260293020116 1102 56-57 -0.06652232009418668 1102 58-59 0.032612006769490165 1102 60-61 0.08344432414880742 1102 62-63 0.07794931111106962 1102 64-65 0.14102888264228497 1102 66-67 0.1237559810161919 1102 68-69 0.2037579918599377 1102 70-71 0.32417068950398686 1102 72-73 0.21313334163147246 1102 74-75 0.19467734723698982 1102 76-77 0.36424978222522597 1102 78-79 0.2923728339530882 1102 80-81 0.26474698218440196 1102 82-83 -0.01778879187125426 1102 84-85 0.06699227444767786 1102 86-87 0.24374033422682473 1102 88-89 0.12309877040044981 1102 90-91 0.21292642292581476 1102 92-93 0.4769662038595719 1102 94-95 0.05190946822013842 1102 96-97 0.201252544129229 1102 98-99 0.3086104640673497 1102 100-101 -0.03178333042762205 1102 102-103 0.3898299696935261 1102 104-105 0.30851478159311796 1102 106-107 0.23623644256529985 1102 108-109 0.30029210178815546 1102 110-111 0.3424416769023182 1102 112-113 0.1108458990279324 1102 114-115 0.022227096973306715 1102 116-117 -0.3913859553564478 1102 118-119 -0.6151303218949522 1102 120-121 -0.8774340879437652 1102 122-123 -0.898756731708044 1102 124-125 -0.7627200549355209 1103 1 0.545360425491829 1103 2 0.5179085846520834 1103 3 0.45987702747945747 1103 4 0.5629856605004591 1103 5 0.5342587125898461 1103 6 0.4856167990556699 1103 7 0.569079084852298 1103 8 0.4318922689798086 1103 9 0.4578257715577365 1103 10-11 0.5366674989809184 1103 12-13 0.539646373582201 1103 14-15 0.5778939503647607 1103 16-17 0.646959256949323 1103 18-19 0.7163271296881248 1103 20-21 0.6292949140613686 1103 22-23 0.7540544215151215 1103 24-25 0.8492409629222593 1103 26-27 1.0394587663635733 1103 28-29 0.8119209525768483 1103 30-31 0.8466203579896501 1103 32-33 0.5977758995756517 1103 34-35 0.7229578459200781 1103 36-37 0.8359243924725206 1103 38-39 0.9400266149136343 1103 40-41 0.9756495200693323 1103 42-43 0.8437209821346983 1103 44-45 0.675291853451661 1103 46-47 0.8030261885399312 1103 48-49 0.885453995390943 1103 50-51 0.7491630284453272 1103 52-53 0.838567459856975 1103 54-55 0.8264114916649028 1103 56-57 0.6830128024391868 1103 58-59 0.8083780138189738 1103 60-61 0.7236812035027 1103 62-63 0.5574218486481897 1103 64-65 0.5239630226883598 1103 66-67 0.5841581347171712 1103 68-69 0.7707627932809302 1103 70-71 0.6880934227059541 1103 72-73 0.6421840202138007 1103 74-75 0.7228301878464656 1103 76-77 0.6643767562198413 1103 78-79 0.5066223690756217 1103 80-81 0.5241312623875594 1103 82-83 0.7191009697229305 1103 84-85 0.8326107696323817 1103 86-87 0.7064876906698849 1103 88-89 0.5172975159251365 1103 90-91 0.5042172558197073 1103 92-93 0.820021489585752 1103 94-95 0.782974384542193 1103 96-97 0.846509406275711 1103 98-99 0.8287250875859868 1103 100-101 0.8527501268730369 1103 102-103 0.711761084566831 1103 104-105 0.7584814769387904 1103 106-107 0.9748053138150539 1103 108-109 0.6744976470131014 1103 110-111 0.6816148888586895 1103 112-113 1.0532288470449167 1103 114-115 0.664417155533993 1103 116-117 0.5157946668855118 1103 118-119 0.307131064133749 1103 120-121 0.46955184083978097 1103 122-123 0.3551600344100194 1103 124-125 0.26834694292742967 1104 1 -0.2302792488078893 1104 2 -0.19253833094516537 1104 3 -0.29571600527502895 1104 4 -0.30344033227932243 1104 5 -0.2089624526582483 1104 6 -0.20872297274332396 1104 7 -0.1706242011367891 1104 8 -0.37798414410089975 1104 9 -0.21120371538526683 1104 10-11 -0.1399544146400018 1104 12-13 -0.3145263318909315 1104 14-15 -0.14036937673213146 1104 16-17 0.08307417433844932 1104 18-19 -0.10781514020041527 1104 20-21 -0.11978608939062951 1104 22-23 0.03770814098072606 1104 24-25 -0.056642591321590885 1104 26-27 0.12705000723948245 1104 28-29 0.16053356423986287 1104 30-31 0.2753141312116085 1104 32-33 0.12658759421846355 1104 34-35 0.1014592557068994 1104 36-37 0.04402605523043235 1104 38-39 -0.07687106354956086 1104 40-41 -0.22249469980470593 1104 42-43 -0.10117545782419768 1104 44-45 -0.14942026017452292 1104 46-47 -0.08596298113515388 1104 48-49 0.0185306245511363 1104 50-51 -0.05984050260950724 1104 52-53 -0.09219817296602528 1104 54-55 0.19039012087779383 1104 56-57 0.11380808031212197 1104 58-59 -0.15106426397490935 1104 60-61 0.10607270261993662 1104 62-63 0.031594422365547103 1104 64-65 -0.11547411663019957 1104 66-67 -0.061392472676963905 1104 68-69 -0.04721660011546902 1104 70-71 0.028800687719524376 1104 72-73 -0.20519996882411462 1104 74-75 -0.08199009727303519 1104 76-77 -0.09102193676284642 1104 78-79 -0.2052112760797158 1104 80-81 -0.2119520336149705 1104 82-83 -0.03847828137723042 1104 84-85 -0.13914079167358295 1104 86-87 -0.026575104133666372 1104 88-89 0.18181107359464121 1104 90-91 -0.12678751373943342 1104 92-93 -0.12306330843264135 1104 94-95 0.09887862444311324 1104 96-97 -0.18980248600076166 1104 98-99 -0.3192498261139818 1104 100-101 -0.06449736317940236 1104 102-103 0.18591718881200237 1104 104-105 -0.04894427868059381 1104 106-107 0.13192078457844758 1104 108-109 0.30068674290495423 1104 110-111 0.3032452348493635 1104 112-113 0.14259220312808552 1104 114-115 0.36369118120001787 1104 116-117 -0.27341914639020004 1104 118-119 0.10083445552745829 1104 120-121 -0.45085449397800303 1104 122-123 -0.5121388403956395 1104 124-125 -0.2576583536548256 1105 1 0.4643488083436864 1105 2 0.4909861008441254 1105 3 0.36774944446311153 1105 4 0.746084252049755 1105 5 0.4981992903630612 1105 6 0.5638783390988493 1105 7 0.44410478650748786 1105 8 0.618679048048385 1105 9 0.5010045522712119 1105 10-11 0.7051032962462571 1105 12-13 0.8928642208115676 1105 14-15 0.7579533211882463 1105 16-17 0.7244690235782869 1105 18-19 0.9400664635012106 1105 20-21 0.7660855483782214 1105 22-23 0.5970365845664247 1105 24-25 0.7728941961904852 1105 26-27 0.8127894438592023 1105 28-29 0.61286805857047 1105 30-31 0.6781717098967164 1105 32-33 0.5984312917829016 1105 34-35 0.682991772104927 1105 36-37 0.6240013946314633 1105 38-39 0.5532231297691936 1105 40-41 0.7360843920750924 1105 42-43 0.4451140118458099 1105 44-45 0.6565681976482196 1105 46-47 0.4720402730469715 1105 48-49 0.4395881066277063 1105 50-51 0.44377596151821663 1105 52-53 0.5369585362422953 1105 54-55 0.723090837814798 1105 56-57 0.5630631776833503 1105 58-59 0.7489236599585851 1105 60-61 0.7327474623697725 1105 62-63 0.5752716481752778 1105 64-65 0.3916380509601787 1105 66-67 0.4501497045742724 1105 68-69 0.5350786152198665 1105 70-71 0.44573323971016876 1105 72-73 0.43208583989099125 1105 74-75 0.28814451908939986 1105 76-77 0.2641955595507781 1105 78-79 0.002773546211429334 1105 80-81 0.14566796435116558 1105 82-83 -0.0326531682435558 1105 84-85 0.10784336961181751 1105 86-87 0.21577883901984052 1105 88-89 0.23232398862997883 1105 90-91 -0.004155058357323327 1105 92-93 0.177891336403885 1105 94-95 -0.07787685427758717 1105 96-97 0.0556244982876386 1105 98-99 0.04452260994642643 1105 100-101 0.05547835757109354 1105 102-103 0.32873574273481765 1105 104-105 0.2798202761574622 1105 106-107 0.42869011899650644 1105 108-109 0.03370397990093821 1105 110-111 0.035096178053713345 1105 112-113 0.16037262210403114 1105 114-115 0.33870007935428603 1105 116-117 0.2178225274797363 1105 118-119 0.09739913239734221 1105 120-121 0.07897021238290591 1105 122-123 0.24698176772964686 1105 124-125 0.018893103100143804 1106 1 -0.3683324644784314 1106 2 -0.544669753473702 1106 3 -0.2863443099827556 1106 4 -1.2164756189608212 1106 5 -0.7682800571022064 1106 6 -0.5055601775227672 1106 7 -0.4254771834265938 1106 8 -0.19854839065792618 1106 9 -0.4371089773916168 1106 10-11 -0.4220959260233883 1106 12-13 -0.29024622638338826 1106 14-15 -0.3696993795278374 1106 16-17 -0.1726387916633243 1106 18-19 -0.15220306274717643 1106 20-21 -0.059420094909114596 1106 22-23 -0.23201982459562487 1106 24-25 -0.11892303717604591 1106 26-27 -0.12463349444401928 1106 28-29 -0.1560802271031676 1106 30-31 -0.3307555912841025 1106 32-33 -0.2932910964183577 1106 34-35 -0.15955339790503587 1106 36-37 -0.2582779718028334 1106 38-39 -0.2851755281717203 1106 40-41 -0.2405422025092534 1106 42-43 -0.35794593029864075 1106 44-45 -0.3241367390426291 1106 46-47 -0.41498727947353586 1106 48-49 -0.33387125221897307 1106 50-51 -0.2964727699276999 1106 52-53 -0.5886579573319466 1106 54-55 -0.5906391854222726 1106 56-57 -0.5966205136904783 1106 58-59 -0.6151424708328967 1106 60-61 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-2.526254691927072 2216 76-77 -2.5323442663287175 2216 78-79 -2.9947347203268784 2216 80-81 -2.9733348713853935 2216 82-83 -3.1478785098922586 2216 84-85 -2.9798695722294255 2216 86-87 -3.300594662016092 2216 88-89 -3.250021981694193 2216 90-91 -3.374455426689156 2216 92-93 -3.4781761980749017 2216 94-95 -3.246032893036144 2216 96-97 -3.2456398111948133 2216 98-99 -3.2344900619243226 2216 100-101 -3.1280296369039284 2216 102-103 -3.1364780885440915 2216 104-105 -3.652918616335686 2216 106-107 -3.665178254692883 2216 108-109 -4.156954831810342 2216 110-111 -4.140846364273052 2216 112-113 -4.29671855048873 2216 114-115 -4.2067501853842195 2216 116-117 -4.032015280945856 2216 118-119 -3.8283419784579813 2216 120-121 -3.3773126183309436 2216 122-123 -2.894703036489428 2216 124-125 -2.07795488677073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25622.0 3 2810.0 4 1486.0 5 2942.0 6 7276.0 7 6251.0 8 3784.0 9 3722.0 10 2841.0 11 1881.0 12 1501.0 13 1220.0 14 1368.0 15 1331.0 16 1293.0 17 1310.0 18 1484.0 19 1845.0 20 2409.0 21 2941.0 22 3656.0 23 4402.0 24 4856.0 25 5492.0 26 5894.0 27 6285.0 28 7203.0 29 8538.0 30 11272.0 31 15033.0 32 20394.0 33 27397.0 34 37607.0 35 55294.0 36 84312.0 37 26690.0 38 1752.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.9539563140102 18.377555730199717 10.636078989059792 14.032408966730287 2 19.8634608197334 23.514388937336488 34.52323408496325 22.098916157966862 3 31.505095049455615 23.361237760669706 24.42384831950569 20.709818870368988 4 16.489014466320832 22.142739680973524 28.62492712024039 32.743318732465255 5 16.275234249070103 34.842758521035705 25.221418623828757 23.66058860606544 6 32.68036382932608 35.87097063505706 8.9626977780103 22.48596775760656 7 29.58417726091625 40.82466984058177 11.435561302152234 18.155591596349748 8 35.61187552472988 29.189306347581073 10.591260024364905 24.60755810332414 9 36.887669813177475 17.63366442698874 14.613960742108187 30.86470501772559 10-11 30.259568457163354 27.832420272199343 14.639122863399576 27.268888407237725 12-13 32.235542368278786 29.118553452068653 17.127058747324966 21.518845432327595 14-15 35.84518568898123 22.786792128491246 20.585231080451525 20.782791102076 16-17 29.969954932398597 24.28206987097562 21.862520148381776 23.88545504824401 18-19 28.970570283285625 24.728510676462456 21.7547714606736 24.546147579578324 20-21 37.56841730257077 22.771221508880235 17.807557106804488 21.852804081744505 22-23 33.5894388846952 25.670720010363805 17.05132325353702 23.688517851403972 24-25 27.050401472856052 27.511790073169458 18.26234376440282 27.175464689571672 26-27 24.361939996387576 27.126939342414218 21.055326145854742 27.455794515343463 28-29 26.29188456565123 25.566994127627556 22.92916030306126 25.211961003659955 30-31 27.538446973943504 26.60333784258477 20.429524884341436 25.428690299130285 32-33 24.393181812519774 26.449001861000454 22.17492906025705 26.982887266222715 34-35 23.02645510554389 27.126635226471546 21.91247069609389 27.934438971890675 36-37 24.090613532238656 28.487507380473698 19.843446762183074 27.578432325104572 38-39 24.892936419554708 25.347598512196157 21.636933011457486 28.122532056791655 40-41 22.91110795546554 25.66125307364031 22.99469104153743 28.43294792935672 42-43 22.82515813521277 25.70983340682666 24.817450055680535 26.647558402280037 44-45 23.446488140170455 25.08227515402457 25.42582530512186 26.045411400683115 46-47 20.983589812579567 25.54329005946731 24.95858215183472 28.514537976118408 48-49 20.01609379242994 25.98001479831688 26.12749970727235 27.87639170198083 50-51 21.216303580125885 26.13164290171937 24.823086399607373 27.82896711854737 52-53 20.84133663181339 25.82642620647389 24.158376868785766 29.17386029292695 54-55 19.737192853957552 25.235988310824446 24.06383206650772 30.962986768710277 56-57 20.37275096289431 24.670274094779693 24.86297752333119 30.093997418994807 58-59 22.149642374008774 23.429547306033676 24.41410872527697 30.006701594680578 60-61 22.31758373578245 23.54255704770719 23.787327492890444 30.35253172361991 62-63 22.130833065518676 23.292401288499914 23.946865571989825 30.629900073991585 64-65 22.560437082776414 23.257143640139166 23.669088675933665 30.513330601150756 66-67 23.707576289807847 24.757658876374265 22.200589441375996 29.334175392441896 68-69 23.833506903785498 25.055434582210108 21.917588084928273 29.193470429076125 70-71 23.591667299970474 25.1243040406444 22.684444023626178 28.59958463575895 72-73 24.89558329482195 24.713469101001127 23.09150677696492 27.299440827211995 74-75 25.72947667530798 24.31406208540075 23.30201947596523 26.654441763326037 76-77 25.492345445259655 23.97488757956626 23.83923566561616 26.693531309557915 78-79 25.39335136232671 24.431943456559136 24.915637016820277 25.259068164293875 80-81 26.028545661099372 25.239790528959222 24.331202415593733 24.40046139434767 82-83 25.78830932966614 24.71904373973896 24.58127489742076 24.91137203317414 84-85 25.930152591869078 24.514865497892018 24.89921062860674 24.655771281632166 86-87 26.29688130627376 24.272271578978838 24.845161515349314 24.585685599398087 88-89 26.48216370298295 23.628032257702817 24.925715837694867 24.964088201619365 90-91 27.460073667990798 23.315159238207702 24.418559749673328 24.806207344128172 92-93 27.974745203370134 23.724833343507342 23.95842027958176 24.342001173540766 94-95 27.2407211768458 24.32576542816138 23.698747209376496 24.73476618561633 96-97 26.90048134897302 24.605602782456177 22.96223951845171 25.531676350119092 98-99 26.3808075259133 25.47170868975414 22.884800560058697 25.262683224273864 100-101 25.99344533481484 26.758031707040598 22.82672738846045 24.421795569684114 102-103 25.504924052745785 28.427840766124312 22.9741378236509 23.093097357479003 104-105 24.524905703622874 30.08316018674918 22.930088641085817 22.46184546854213 106-107 23.683397567969546 31.480494179021555 22.524184527383387 22.31192372562551 108-109 23.294145197896345 33.31763814876544 22.359783456278944 21.02843319705927 110-111 22.75814218840699 35.63541830158173 21.284787878334914 20.32165163167637 112-113 22.481763463148244 37.27528201857535 20.562318132897516 19.68063638537888 114-115 22.05536482183699 38.208254645293415 20.58205065599665 19.154329876872943 116-117 21.707561342013022 39.26524114530004 20.179273885953307 18.847923626733632 118-119 21.705979066349723 39.90344643620622 20.028943632213938 18.361630865230122 120-121 22.0507211804963 39.90814431333079 19.568350521499635 18.47278398467328 122-123 22.374905325679663 40.29750159451487 19.47114426373276 17.85644881607271 124-125 22.536538956158246 42.306530191303565 18.292701198362895 16.864229654175293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 0.5 3 1.0 4 4.0 5 4.5 6 5.0 7 9.0 8 11.5 9 12.0 10 12.5 11 16.0 12 26.0 13 37.5 14 42.5 15 53.5 16 83.0 17 102.5 18 134.5 19 183.0 20 238.5 21 330.5 22 481.0 23 651.0 24 835.0 25 1031.5 26 1250.5 27 1490.5 28 1730.5 29 1939.5 30 2101.5 31 2273.0 32 2340.5 33 2350.5 34 2332.5 35 2287.5 36 2257.0 37 2104.0 38 1941.5 39 1820.0 40 1653.0 41 1521.0 42 1482.0 43 1509.0 44 1638.5 45 1997.5 46 2598.0 47 3462.0 48 5297.0 49 9192.5 50 15859.0 51 25239.0 52 35493.0 53 42726.0 54 43808.0 55 39783.0 56 33424.5 57 26106.5 58 19301.5 59 13848.5 60 9854.5 61 6831.5 62 4738.5 63 3900.5 64 3363.5 65 2769.5 66 2362.0 67 2091.0 68 1663.0 69 1333.5 70 1081.5 71 745.5 72 516.5 73 356.5 74 272.0 75 211.5 76 175.0 77 155.0 78 118.0 79 92.0 80 74.5 81 61.0 82 46.5 83 28.0 84 17.0 85 12.0 86 14.0 87 13.0 88 7.5 89 5.0 90 2.0 91 4.0 92 4.0 93 1.5 94 2.5 95 1.5 96 0.5 97 0.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14499360981771164 2 0.011709105947478432 3 0.006726507671955695 4 0.012705625602582978 5 0.001993039310209095 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011210846119926157 28-29 0.0064773777581795585 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.2456495688806842E-4 52-53 0.0 54-55 0.0 56-57 7.473897413284105E-4 58-59 0.0 60-61 1.2456495688806842E-4 62-63 0.0 64-65 0.002117604267097163 66-67 0.0 68-69 0.005729988016851147 70-71 0.015570619611008554 72-73 1.2456495688806842E-4 74-75 0.007598462370172174 76-77 4.982598275522737E-4 78-79 0.0016193444395448896 80-81 0.0012456495688806842 82-83 0.0 84-85 0.016193444395448894 86-87 0.002740429051537505 88-89 0.016567139266113102 90-91 3.736948706642053E-4 92-93 0.0118336709043665 94-95 0.013203885430135252 96-97 0.0062282478444034214 98-99 0.0026158640946494366 100-101 8.71954698216479E-4 102-103 0.0 104-105 7.473897413284105E-4 106-107 9.965196551045474E-4 108-109 0.0 110-111 0.0 112-113 0.0012456495688806842 114-115 0.005729988016851147 116-117 0.0 118-119 0.06788790150399729 120-121 0.0016193444395448896 122-123 0.005231728189298873 124-125 0.23268733946691184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 401397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.6308619098632 #Duplication Level Percentage of deduplicated Percentage of total 1 85.2827042533421 57.677427946574824 2 7.621336275313641 10.30875082408736 3 2.578680359912637 5.231951259927836 4 1.3310367210045506 3.6007664270086353 5 0.8107724519861516 2.74166198702983 6 0.5325419950082061 2.160976447536182 7 0.3788067810468784 1.7933320369650851 8 0.27113910754285314 1.4669897232475397 9 0.21557630343156356 1.3121650087576957 >10 0.9417306578920832 10.242083497635607 >50 0.022434852629451326 1.0680715533403584 >100 0.01140131854411413 1.4732621817033629 >500 0.0018389223458248594 0.9225611061856785 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 864 0.21524824550258223 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 813 0.20254261989999925 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 738 0.183857876366789 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 683 0.17015573110910148 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 611 0.1522183773172196 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 445 0.11086281163038089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.4912991377613685E-4 0.0 0.0 0.0 0.0 7 2.4912991377613685E-4 0.0 0.0 0.0 0.0 8 2.4912991377613685E-4 0.0 0.0 0.0 0.0 9 2.4912991377613685E-4 0.0 0.0 0.0 0.0 10-11 0.002242169223985232 0.0 0.0 6.228247844403421E-4 0.0 12-13 0.0047334683617466 0.0 0.0 0.001494779482656821 0.0 14-15 0.0064773777581795585 0.0 0.0 0.001993039310209095 0.0 16-17 0.007224767499507969 0.0 0.0 0.0026158640946494366 0.0 18-19 0.007723027327060242 0.0 0.0 0.0037369487066420525 0.0 20-21 0.008096722197724449 0.0 0.0 0.007224767499507968 0.0 22-23 0.008968676895940926 0.0 0.0 0.025535816162054026 0.0 24-25 0.009716066637269337 0.0 0.0 0.05804726990983988 0.0 26-27 0.010214326464821611 0.0 0.0 0.13403189361156162 0.0 28-29 0.011459976033702295 0.0 0.0 0.42887714656561954 0.0 30-31 0.012331930731918773 0.0 0.0 1.0515773660490737 0.0 32-33 0.012456495688806843 0.0 0.0 1.9912954008126618 0.0 34-35 0.013328450387023322 0.0 0.0 3.3606379718831976 0.0 36-37 0.01569518456789662 0.0 0.0 5.204822158611051 0.0 38-39 0.016691704223001168 0.0 0.0 7.617147113705384 0.0 40-41 0.017065399093665373 0.0 0.0 10.416744519764721 0.0 42-43 0.017937353791881855 0.0 0.0 12.68370217017068 0.0 44-45 0.018435613619434126 0.0 0.0 14.67051323253537 0.0 46-47 0.0189338734469864 0.0 0.0 16.973719285395752 0.0 48-49 0.0189338734469864 0.0 0.0 19.431385884797344 0.0 50-51 0.0189338734469864 0.0 0.0 21.783172270844076 0.0 52-53 0.01918300336076254 0.0 0.0 23.832141246695915 0.0 54-55 0.01918300336076254 0.0 0.0 25.86441851832475 0.0 56-57 0.01918300336076254 0.0 0.0 27.91126988990949 0.0 58-59 0.019556698231426743 0.0 0.0 29.90343724542037 0.0 60-61 0.01980582814520288 0.0 0.0 31.967354016098774 0.0 62-63 0.020179523015867083 0.0 0.0 33.94918248018794 0.0 64-65 0.020179523015867083 0.0 0.0 35.80295816859618 0.0 66-67 0.020179523015867083 0.0 0.0 37.779554904496045 0.0 68-69 0.020179523015867083 0.0 0.0 39.84172776577802 0.0 70-71 0.020179523015867083 0.0 0.0 42.08800763334055 0.0 72-73 0.020179523015867083 0.0 0.0 44.25718179258938 0.0 74-75 0.020179523015867083 0.0 0.0 46.14782870823649 0.0 76-77 0.020179523015867083 0.0 0.0 48.04171431276268 0.0 78-79 0.020179523015867083 0.0 0.0 49.960388343709596 0.0 80-81 0.020179523015867083 0.0 0.0 51.81242012272139 0.0 82-83 0.020179523015867083 0.0 0.0 53.70854789647157 0.0 84-85 0.020179523015867083 0.0 0.0 55.69361504943983 0.0 86-87 0.020179523015867083 0.0 0.0 57.3277827188544 0.0 88-89 0.020179523015867083 0.0 0.0 58.787310318711896 0.0 90-91 0.020179523015867083 0.0 0.0 60.47877786829498 0.0 92-93 0.020179523015867083 0.0 0.0 61.98526645689928 0.0 94-95 0.020179523015867083 0.0 0.0 63.3463877408152 0.0 96-97 0.020179523015867083 0.0 0.0 64.62193289934902 0.0 98-99 0.020179523015867083 0.0 0.0 65.80355608038924 0.0 100-101 0.020179523015867083 0.0 0.0 67.00610617418666 0.0 102-103 0.020179523015867083 0.0 0.0 68.12233275286064 0.0 104-105 0.020304087972755153 0.0 0.0 69.1770242428319 0.0 106-107 0.020428652929643222 0.0 0.0 70.17267194323824 0.0 108-109 0.020428652929643222 0.0 0.0 71.08436286270201 0.0 110-111 0.020428652929643222 0.0 0.0 71.97886381811523 0.0 112-113 0.020428652929643222 0.0 0.0 72.84845178215085 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTATG 15 2.6992543E-4 119.01283 2 ACCTAAG 25 0.0020397913 71.54144 1 TTCTAGG 35 9.14029E-5 68.00733 2 GTTCTAG 40 1.7533413E-4 59.61787 1 CTAGGCA 30 0.0042369165 59.49159 4 TGTGCAA 15 0.00424055 59.484177 10-11 CTTAATC 35 0.007796933 50.99279 4 TTAATCA 35 0.007800774 50.98644 5 TAACAAC 145 0.0 49.33893 1 TGGAAAC 85 6.182745E-8 49.005287 2 TAGAACA 50 5.332144E-4 47.593273 4 CTATGGG 75 1.469838E-6 47.59327 3 GTACCGA 25 5.3386315E-4 47.58734 36-37 TATAAGG 105 6.361006E-9 45.338226 2 TCTATGG 80 2.2884415E-6 44.629814 2 GGAAACA 375 0.0 44.51467 1 ACAACAC 270 0.0 44.062355 5 TTAGGGC 55 8.527702E-4 43.26661 4 GTTATAG 125 6.0936145E-10 42.924866 1 TTATAGG 125 6.202754E-10 42.844624 2 >>END_MODULE